LOCUS       DAZPFP010000001        72983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPFP010000001 DAZPFP010000000
VERSION     DAZPFP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.001"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUR4_000001"
     CDS             complement(<1..209)
                     /locus_tag="ACHUR4_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1453772.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUR4_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUR4_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1453773.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUR4_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUR4_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1453774.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUR4_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUR4_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1453775.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUR4_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUR4_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1453776.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUR4_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUR4_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1453777.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUR4_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUR4_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1453778.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHUR4_000008"
     CDS             complement(4598..5320)
                     /locus_tag="ACHUR4_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1453779.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUR4_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUR4_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1453780.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHUR4_000010"
     CDS             6520..6738
                     /locus_tag="ACHUR4_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1453781.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUR4_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUR4_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1453782.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHUR4_000012"
     CDS             complement(7472..7672)
                     /locus_tag="ACHUR4_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1453783.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUR4_000013"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUR4_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUR4_000014"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUR4_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1453784.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHUR4_000015"
     CDS             10101..12014
                     /locus_tag="ACHUR4_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1453785.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHUR4_000016"
     CDS             12014..13036
                     /locus_tag="ACHUR4_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1453786.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHUR4_000017"
     CDS             13030..13248
                     /locus_tag="ACHUR4_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1453787.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHUR4_000018"
     CDS             13302..14171
                     /locus_tag="ACHUR4_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1453788.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHUR4_000019"
     CDS             complement(14226..14630)
                     /locus_tag="ACHUR4_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1453789.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHUR4_000020"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHUR4_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1453790.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHUR4_000021"
     CDS             15615..17705
                     /locus_tag="ACHUR4_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1453791.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUR4_000022"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUR4_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1453792.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUR4_000023"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUR4_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1453793.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHUR4_000024"
     CDS             complement(19673..20275)
                     /locus_tag="ACHUR4_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1453794.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHUR4_000025"
     CDS             complement(20265..20432)
                     /locus_tag="ACHUR4_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1453795.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUR4_000026"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUR4_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1453796.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUR4_000027"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUR4_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUR4_000028"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUR4_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1453797.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUR4_000029"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUR4_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1453798.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUR4_000030"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUR4_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1453799.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUR4_000031"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUR4_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1453800.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28268..28435
                     /locus_tag="ACHUR4_000032"
     CDS             28268..28435
                     /locus_tag="ACHUR4_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1453801.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28730..30067
                     /gene="frlA"
                     /locus_tag="ACHUR4_000033"
                     /pseudo
     CDS             28730..30067
                     /gene="frlA"
                     /locus_tag="ACHUR4_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30088..31110
                     /gene="frlB"
                     /locus_tag="ACHUR4_000034"
     CDS             30088..31110
                     /gene="frlB"
                     /locus_tag="ACHUR4_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1453802.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31160..31990
                     /gene="frlC"
                     /locus_tag="ACHUR4_000035"
     CDS             31160..31990
                     /gene="frlC"
                     /locus_tag="ACHUR4_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR1453803.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31987..32772
                     /gene="frlD"
                     /locus_tag="ACHUR4_000036"
     CDS             31987..32772
                     /gene="frlD"
                     /locus_tag="ACHUR4_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1453804.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32872..33603
                     /gene="frlR"
                     /locus_tag="ACHUR4_000037"
                     /gene_synonym="yhfR"
     CDS             32872..33603
                     /gene="frlR"
                     /locus_tag="ACHUR4_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1453805.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33763..34848)
                     /locus_tag="ACHUR4_000038"
     CDS             complement(33763..34848)
                     /locus_tag="ACHUR4_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1453806.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34860..36164)
                     /locus_tag="ACHUR4_000039"
     CDS             complement(34860..36164)
                     /locus_tag="ACHUR4_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1453807.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36176..36529)
                     /locus_tag="ACHUR4_000040"
     CDS             complement(36176..36529)
                     /locus_tag="ACHUR4_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1453808.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36540..37418)
                     /locus_tag="ACHUR4_000041"
     CDS             complement(36540..37418)
                     /locus_tag="ACHUR4_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1453809.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37415..38641)
                     /locus_tag="ACHUR4_000042"
     CDS             complement(37415..38641)
                     /locus_tag="ACHUR4_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1453810.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38642..39805)
                     /locus_tag="ACHUR4_000043"
     CDS             complement(38642..39805)
                     /locus_tag="ACHUR4_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR1453811.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39889..40251)
                     /locus_tag="ACHUR4_000044"
     CDS             complement(39889..40251)
                     /locus_tag="ACHUR4_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1453812.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40268..41173)
                     /gene="yhfZ"
                     /locus_tag="ACHUR4_000045"
     CDS             complement(40268..41173)
                     /gene="yhfZ"
                     /locus_tag="ACHUR4_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1453813.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="ACHUR4_000046"
     CDS             complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="ACHUR4_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1453814.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42440..43198)
                     /gene="gph"
                     /locus_tag="ACHUR4_000047"
     CDS             complement(42440..43198)
                     /gene="gph"
                     /locus_tag="ACHUR4_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1453815.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="ACHUR4_000048"
     CDS             complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="ACHUR4_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1453816.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43886..44722)
                     /gene="dam"
                     /locus_tag="ACHUR4_000049"
     CDS             complement(43886..44722)
                     /gene="dam"
                     /locus_tag="ACHUR4_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1453817.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44829..46121)
                     /gene="damX"
                     /locus_tag="ACHUR4_000050"
     CDS             complement(44829..46121)
                     /gene="damX"
                     /locus_tag="ACHUR4_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1453818.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="ACHUR4_000051"
     CDS             complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="ACHUR4_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1453819.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="ACHUR4_000052"
     CDS             complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="ACHUR4_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1453820.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="ACHUR4_000053"
     CDS             complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="ACHUR4_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1453821.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="ACHUR4_000054"
                     /pseudo
     CDS             complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="ACHUR4_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="ACHUR4_000055"
     CDS             complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="ACHUR4_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1453822.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="ACHUR4_000056"
     CDS             complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="ACHUR4_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1453823.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="ACHUR4_000057"
     CDS             complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="ACHUR4_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1453824.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51688..54240
                     /gene="mrcA"
                     /locus_tag="ACHUR4_000058"
     CDS             51688..54240
                     /gene="mrcA"
                     /locus_tag="ACHUR4_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1453825.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="ACHUR4_000059"
     CDS             complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="ACHUR4_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1453826.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55285..57420
                     /gene="igaA"
                     /locus_tag="ACHUR4_000060"
     CDS             55285..57420
                     /gene="igaA"
                     /locus_tag="ACHUR4_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1453827.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57485..58153
                     /gene="yrfG"
                     /locus_tag="ACHUR4_000061"
     CDS             57485..58153
                     /gene="yrfG"
                     /locus_tag="ACHUR4_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1453828.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58164..58565
                     /gene="hslR"
                     /locus_tag="ACHUR4_000062"
     CDS             58164..58565
                     /gene="hslR"
                     /locus_tag="ACHUR4_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1453829.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58590..59459
                     /gene="hslO"
                     /locus_tag="ACHUR4_000063"
     CDS             58590..59459
                     /gene="hslO"
                     /locus_tag="ACHUR4_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1453830.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59603..61361)
                     /locus_tag="ACHUR4_000064"
                     /pseudo
     CDS             complement(59603..61361)
                     /locus_tag="ACHUR4_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            61740..63362
                     /gene="pckA"
                     /locus_tag="ACHUR4_000065"
     CDS             61740..63362
                     /gene="pckA"
                     /locus_tag="ACHUR4_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1453831.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63438..64790)
                     /gene="envZ"
                     /locus_tag="ACHUR4_000066"
     CDS             complement(63438..64790)
                     /gene="envZ"
                     /locus_tag="ACHUR4_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1453832.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64787..65506)
                     /gene="ompR"
                     /locus_tag="ACHUR4_000067"
     CDS             complement(64787..65506)
                     /gene="ompR"
                     /locus_tag="ACHUR4_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1453833.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65734..66210
                     /gene="greB"
                     /locus_tag="ACHUR4_000068"
     CDS             65734..66210
                     /gene="greB"
                     /locus_tag="ACHUR4_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1453834.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66307..68628
                     /locus_tag="ACHUR4_000069"
     CDS             66307..68628
                     /locus_tag="ACHUR4_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1453835.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69066..69293
                     /gene="feoA"
                     /locus_tag="ACHUR4_000070"
     CDS             69066..69293
                     /gene="feoA"
                     /locus_tag="ACHUR4_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1453836.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69310..71631
                     /gene="feoB"
                     /locus_tag="ACHUR4_000071"
     CDS             69310..71631
                     /gene="feoB"
                     /locus_tag="ACHUR4_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1453837.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71631..71867
                     /gene="feoC"
                     /locus_tag="ACHUR4_000072"
     CDS             71631..71867
                     /gene="feoC"
                     /locus_tag="ACHUR4_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1453838.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72070..72957
                     /gene="rpnA"
                     /locus_tag="ACHUR4_000073"
     CDS             72070..72957
                     /gene="rpnA"
                     /locus_tag="ACHUR4_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR1453839.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17592 a        19781 c        18455 g        17155 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28261 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28321 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28381 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28441 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28501 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28561 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28621 acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28681 ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28741 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28801 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28861 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28921 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    28981 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29041 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29101 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29161 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29221 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29281 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29341 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29401 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29461 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29521 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggctctttgt
    29581 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29641 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgcgca
    29701 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29761 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29821 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29881 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    29941 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg ccatcgttat
    30001 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30061 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30121 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30181 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30241 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30301 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30361 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30421 ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg cgcggacagc
    30481 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30541 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30601 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30661 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30721 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30781 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30841 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30901 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    30961 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31021 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31081 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31141 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31201 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31261 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31321 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31381 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31441 ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31501 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31561 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31621 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31681 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31741 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31801 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31861 tattattgat cggggctatg agggttactc tacggtggag ctggtgacga tgtatatgaa
    31921 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    31981 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32041 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32101 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32161 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32221 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32281 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32341 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32401 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32461 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32521 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32581 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32641 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32701 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32761 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32821 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32881 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    32941 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33001 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33061 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33121 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc gacgaaagag
    33181 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33241 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33301 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33361 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33421 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33481 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33541 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33601 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33661 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33721 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33781 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33841 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33901 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    33961 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34021 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34081 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34141 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34201 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34261 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34321 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34381 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34441 atcaggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga aatcagcagt
    34501 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34561 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34621 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34681 ctccacccgt tgggtaatac gcggttgatt cagacttgga gttaacccta aatcaccgcc
    34741 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34801 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gtgccttcct
    34861 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34921 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    34981 taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35041 gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat gttatccgac
    35101 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35161 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35221 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35281 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35341 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35401 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35461 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35521 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35581 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35641 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35701 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35761 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35821 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35881 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    35941 agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36001 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36061 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gctccagaag cgatgtcata
    36121 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36181 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36241 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta atgcctggtt
    36301 tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac aatgccgcgc
    36361 ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc accttcattg
    36421 ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg
    36481 tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct
    36541 tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat
    36601 cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc ataaccacca
    36661 ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg gttcagcaac
    36721 ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta gtaactgttc
    36781 ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa aatgttgtcg
    36841 aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac tccgtgggct
    36901 tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt ggagatcggg
    36961 cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc cagcggcgta
    37021 atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc cgtgccatcg
    37081 ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcacc
    37141 acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac gttgatcccg
    37201 gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc
    37261 tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca aatgaacgca
    37321 tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat atgcagatgc
    37381 tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga
    37441 gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg
    37501 tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc agcccttgct
    37561 gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg gtcggatcgt
    37621 taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg
    37681 caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac
    37741 cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg gtgttaaatt
    37801 cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag ctcacaccat
    37861 aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat
    37921 acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc acgacctgga
    37981 agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc ccttctttgc
    38041 tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat gtaatgaccc
    38101 gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga
    38161 tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg agattatcgc
    38221 caatcgcaac cgcctgattg accgacagaa attgcagatc atcgccacgg cgctccacct
    38281 gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga aaaggcatcc
    38341 gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag gtatcgccac
    38401 cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc atatcgcctg
    38461 gcgcataacc caatgtgttg attagcccca gcttctccag cgttggtagc tgcaaatgcg
    38521 gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcggg cgcaccagcg
    38581 tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca
    38641 tttatgcctc cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccgc
    38701 gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc gtgcgaaaac
    38761 agagcaccac tgcactgctc accggatact cgcctgccag cggcagggtg tagtcgatac
    38821 tgctgtcatc cacagttttg agattggttt cagtaatctt ttgattttct ggcgtaaaca
    38881 ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc
    38941 cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc
    39001 cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca tgagtcgcac
    39061 cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg ttcagttgct
    39121 caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg
    39181 gtgttggcaa aactttccca gcagcctcat cccaaagcag gcaagggaaa tgggttagtc
    39241 cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc agcgaatgct
    39301 gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca
    39361 gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgc gctttgtcga
    39421 gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc tgatgacaag
    39481 ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc atgactcgcg
    39541 cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat ttttccgcca
    39601 gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg taaagccgcg
    39661 ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc acccagctgt
    39721 ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct
    39781 ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat
    39841 tcattttttt gaatatttag gttgtgcgac gtggatttgt cgggagcttc aaacctcttc
    39901 gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt cacacgggtt
    39961 aacttccaga gcgaattcct tcaacaactc ctgatgcaat tgcacaacgg cttgccagtg
    40021 gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt cttcaccacg
    40081 gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg
    40141 caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc ctttgcagac
    40201 gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca tatcagatgc
    40261 ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga tgggcaagca
    40321 gggcgtgttt atctacaaca gcacgcagca attgttgcat tgggtaatca tcgactcgcg
    40381 tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc gtctgttagc ggcgtcgcct
    40441 ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc gcatcgacat
    40501 cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac
    40561 tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta tcgagaccta
    40621 ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca ccaacatacg
    40681 tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa ctttccgccg
    40741 ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc acgcgaatat
    40801 ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc
    40861 tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc acgttgttga
    40921 tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg ccattgcgcc
    40981 cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca
    41041 ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc
    41101 atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct tttttgataa
    41161 acgttcgacg cataatatat tttcaataaa atgaatattt atatcttcat taaaatgaag
    41221 ataagtggcg acgacgagat gtggaaacgg cgaggcgctt cacatttttt cgggttgttg
    41281 cgaaaagagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat
    41341 tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc
    41401 ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa
    41461 accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc
    41521 gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc
    41581 ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata
    41641 catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag
    41701 gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg
    41761 ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag
    41821 caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag
    41881 cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc
    41941 gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg
    42001 gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac
    42061 cggatagcca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa
    42121 ctgcgtcatg cgactcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg
    42181 cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca
    42241 agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg catcctggcg
    42301 cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt
    42361 tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc
    42421 gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc
    42481 ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga
    42541 tagcctcgcc gtagttgtat ccgtaggtta agccaactga tgggcaacct gccgcttttg
    42601 ccgcctgaat atcattccgt gagtcgccga caaacagcat ctgttgtggg gcaattccca
    42661 tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc
    42721 cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga
    42781 acggcgtcgg tttgttggtg accaagccta gcggcaggcc tttagcctgc aacgcgccca
    42841 acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc
    42901 gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg
    42961 gcggtttccc cattgtttta cgcagagtcg cacgttcctg acgcgcccag gtcaatgcgc
    43021 gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga
    43081 cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagacca ggagcactgt
    43141 cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga
    43201 cttacctttg ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg
    43261 aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg
    43321 ttcaccttca cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacgg
    43381 cgacgtactt cgcgcagttt atccagtgtt tgaggaatga aagactgacc gccgaaacca
    43441 gggttgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc
    43501 agaggtgtcg ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc
    43561 agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg
    43621 gcagcgaaat caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg
    43681 gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga
    43741 acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt
    43801 gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac
    43861 tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt
    43921 acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc
    43981 gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt
    44041 gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat
    44101 gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag
    44161 acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgctccatgc
    44221 tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca
    44281 actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc
    44341 gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg
    44401 cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg
    44461 cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca
    44521 tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag
    44581 aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc
    44641 ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca
    44701 aaaaagcgcg atttttcttc atgccgatta actaattaca ccttctccgg ctgtggagaa
    44761 agctccgaca gcatccagcg cttcaggcag ctcccgcaac gataaaccat tgcgggagat
    44821 aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg
    44881 gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac
    44941 acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag
    45001 tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc
    45061 agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc
    45121 ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc
    45181 gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc
    45241 acaggggcag ctggttcagg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc
    45301 gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg aactgggcgc
    45361 gtagtggacg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg
    45421 cgaacaggcg caaccgttgc gggttcagtc ggcaacgtgg aattgaccgc cacattgttc
    45481 agctgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca
    45541 cgttgttgac catccgttgc caccggggtt tgcccttgag tcggcgtaga agagatcggt
    45601 ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg
    45661 ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt
    45721 tgatcgctgg aagtggtcga gggggctttt agcgcagaac cgataccgat gatcaacagc
    45781 agtagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct
    45841 gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt ttcatcttcg
    45901 gattcgtcaa tttcttcttc gatttccggt tcctcattgc gctctttttg cgcacgagtc
    45961 ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca
    46021 cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg
    46081 ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg
    46141 ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga
    46201 cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga
    46261 aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc
    46321 cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg
    46381 cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc
    46441 agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac
    46501 cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt
    46561 gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc
    46621 tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa
    46681 caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta
    46741 tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc
    46801 gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg
    46861 atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg
    46921 gacttgaatg aaacgaacac cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc
    46981 cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa
    47041 cgccgtaaag acggtatcga gtacagctag gcttttatac tgctcgccgt cagggaggat
    47101 aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag
    47161 aggagccagg gtttcgctgg tgaccaacat gacctgctcg cccgatttca gcggtaagaa
    47221 tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag
    47281 agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag
    47341 tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcaaccact
    47401 ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg
    47461 cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg
    47521 cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg
    47581 acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga gccgccgcca
    47641 gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg
    47701 cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc
    47761 tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca
    47821 atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt
    47881 ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc
    47941 aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata
    48001 ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa
    48061 gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa
    48121 agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc
    48181 accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa
    48241 acacgttcag cataaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg
    48301 tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa
    48361 ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt
    48421 acgggtaaaa atgccgccca gcgccaacgt ttctccgctt tttacctcga cctgcgtttc
    48481 gatctcctgc ttatcaatcg ccagcacttc gccatcggcc tgctgtagca cctgccccgg
    48541 aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcat
    48601 gacctccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct
    48661 ggaaacctga tatggaattt cgctctcctg tttaatgctg gcaggctgaa gatgtgaggc
    48721 cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc
    48781 aagatccagc aagcgtccgt tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt
    48841 cgctacggag aggtcgctac caagcgtggt gacttgccca acgccaccag cgtgttgcgc
    48901 atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat
    48961 atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag
    49021 cgcgcttaac gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt
    49081 catactccct ttggcactca gtagcttctc ccccgctttc gccagttctc ccgcgtcggc
    49141 gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc gcgcctgctc
    49201 cgcctcctgg cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt
    49261 gccttcctgc cgcgttatca gtccggcgct tttcactaca gtttgtagtg cctgcttcca
    49321 gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca cgaccaggtt
    49381 caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat
    49441 cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc
    49501 ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccattgt ggcggttcgc
    49561 agtttgtccc ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt gtccagccgt
    49621 tttccagcac atcgttttgc tgcacccgtc gccatttctt ttgcccgtct tttattacac
    49681 cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa
    49741 tccggcatat agatcttccg gcgatttaaa agggtcacgc ataccggtta aaaggcacaa
    49801 tgcaatacct gccaacaacc agcgtttaac cctcatgcgg catctccagt tgtagcgtga
    49861 acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg
    49921 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcacctc
    49981 cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg
    50041 aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg
    50101 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50161 tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50221 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50281 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50341 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50401 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50461 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50521 tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt
    50581 ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct
    50641 atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50701 agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc
    50761 cagggcaaaa aattaattgg cggattcatc agtacgcctt cccaagcgcc agccccagcg
    50821 cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50881 aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg
    50941 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg
    51001 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51061 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcactaaca tgaacacgca
    51121 acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51181 aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca
    51241 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg
    51301 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51361 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa
    51421 caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa
    51481 agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt tgtaaatgca
    51541 aaccaatttg ccagatctta aatgtcattg tgatgatctc cttatcaccc gtcactctga
    51601 cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc
    51661 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51721 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51781 caactgccgg atgtggtgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51841 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51901 gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51961 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    52021 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc
    52081 agtccagaac gcacgatgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52141 cagttgttga cgaaagacga aatcctcgag ctttatctga acaagattta ccttggttac
    52201 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52261 acgctgaacg aaatggcggt gatagccggg ttgccgaaag cgccttccac ctttaatccg
    52321 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52381 gaagggtata tcacccagca acagttcgat cagacacgca ctgaggcgat taacgctaat
    52441 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52501 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52561 acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct ggactacgac
    52621 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52681 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52741 gccgcagtca ccagcgccaa tcctcaggga gcgacggcga tgctggcgga cgggtcgacc
    52801 gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52861 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52921 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52981 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    53041 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53101 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53161 tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat
    53221 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53281 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53341 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53401 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctagtgga cccgtggttt
    53461 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53521 tgcccggaat gcgatattcc agtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53581 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53641 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53701 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53761 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53821 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53881 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53941 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    54001 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54061 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54121 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54181 gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga
    54241 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54301 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54361 ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54421 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54481 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54541 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54601 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54661 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54721 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54781 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54841 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54901 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54961 ttcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    55021 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    55081 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    55141 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taagctccac
    55201 gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga
    55261 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55321 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55381 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55441 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacggaagct
    55501 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55561 cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55621 gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc
    55681 gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg
    55741 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55801 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55861 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55921 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55981 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    56041 tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56101 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56161 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56221 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56281 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct agtgctgttt
    56341 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56401 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56461 ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56521 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    56581 gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56641 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    56701 cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56761 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56821 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56881 gatggcgtga atgtgttatt acgcccggtg agtgctgaat cgctggataa cctggtggca
    56941 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    57001 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57061 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57121 ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc
    57181 actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg
    57241 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57301 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc
    57361 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57421 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57481 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57541 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57601 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57661 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57721 ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat
    57781 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca
    57841 tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt
    57901 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57961 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    58021 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58081 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58141 ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt cgactggata
    58201 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58261 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58321 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58381 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58441 agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58501 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58561 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58621 tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58681 tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58741 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg
    58801 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58861 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58921 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc
    58981 tggaaggtga taccctggcg gcctgcctag aagattactt tatgcgttct gaacagctgc
    59041 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59101 tgttgcaggt aatgcctgcg caaaataccc aacaggacga ctttgaccac ctggcgacgc
    59161 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59221 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59281 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata
    59341 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59401 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgtaag
    59461 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgact taatggcctg
    59521 atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc
    59581 gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59641 ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga
    59701 gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt
    59761 ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc
    59821 gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa
    59881 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    59941 taacagaacc aggggcaatt aacacatttt cggcgaatac tttttccgat acctgttcct
    60001 gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca
    60061 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    60121 cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat
    60181 gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca
    60241 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    60301 cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac accagctccg
    60361 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacc
    60421 cccaggtggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    60481 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    60541 tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg
    60601 cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg
    60661 gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat agagtcgatt aaacgtgact
    60721 gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc aaatgtaaga tacataaacc
    60781 agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac
    60841 cccacagtaa aagcaccagc cacgtcaggc cattggagag aaaaatcacc agtaatataa
    60901 gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac
    60961 gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa
    61021 aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc
    61081 aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccctgt cgtggcaata
    61141 aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt
    61201 aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa caatccaact gtgatttttt
    61261 ccggcaagcc aggcgataag caggtagcaa accagcccct gaagtaatcc ggtagcaatc
    61321 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    61381 aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa
    61441 aaaaagttag cgtggtgaat cgatacttta ccggttgaat ttgcatcaat ttcattcagg
    61501 aatgcgattc cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca
    61561 ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac
    61621 ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt
    61681 taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa
    61741 tgcgcgttaa caatggtttg cccccgcaag aactcgaggc ttatggtatc agtgacgtac
    61801 atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc
    61861 tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga
    61921 tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata
    61981 ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc tctccggaaa
    62041 cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg
    62101 tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc atcaccgaag
    62161 tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg
    62221 caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac ccgcagtgga
    62281 aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc
    62341 tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact
    62401 acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggcgagaaag
    62461 gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt tccaccgacc
    62521 cgaaacgccg cctgattggc gatgacgaac acggctggga cgatgacggc gtgtttaatt
    62581 tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct
    62641 acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta
    62701 tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatcca atctatcaca
    62761 tcgataacat cgttaagccg gtttccaaag cgggccacgc gactaaggtt atcttcctga
    62821 ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat caaacccagt
    62881 atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac
    62941 cgacgccaac cttctccgct tgcttcggcg cggcgttcct gtcgctgcac ccgactcagt
    63001 acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca
    63061 ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgcc attatcgacg
    63121 ccatcctcaa cggttcgctg gataatgcag aaaccttcac tctgccgatg tttaacctgg
    63181 cgatcccaac cgaactgccg ggcgtagaca cgaagattct cgatccgcgt aacacctacg
    63241 cttctccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact
    63301 tcgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt
    63361 aatgatttga agctggagaa tatctatcca gtatcttata gaaagcaaaa cgggaggcac
    63421 cttcgcctcc cgtttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcact
    63481 ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc cagcatcccg
    63541 ttatggttat ccacgatacg ctgcacaatt gccagcccta atcccgtgcc gctaatggtg
    63601 cgcgcactgt cgccgcggac aaacggctgg aacaggtgct tacgttgttc cggcgcaatt
    63661 cccggaccgt catcttccac ctggaaccag gcgcgattcg gctccgttcc gctgctgact
    63721 ttgatccagc cattgccata acgggcggcg ttgaccacca tattcgccac cgcgcgtttg
    63781 atcgacagcg ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc
    63841 cgctcatagc cactttcggc agcaatcacc tcaccgagca ctgcattaag atccgccatt
    63901 tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg
    63961 cactcttcga tatctttatt gatcgattct gccagatagc catcctgctc gctcatcatc
    64021 tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt cgtgacttac ccccgccatc
    64081 agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatggtt aaaggcacgg
    64141 gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat aatcccttta
    64201 ccaacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat acgaataaac
    64261 agccacgccc cgcctatcgc caatagcata atcgccagcg tatagcggaa cagcggagag
    64321 aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc agatattggg cgacagccag
    64381 gttttcagcc agacgacagg cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg
    64441 cccagttgct gtgccatctg atggcttaag aattcatagt gttgcgccca acgcagacct
    64501 gcctcttcgg cagcctcgtt ggagtagaga gagatcccca gctcacggta gatctcccga
    64561 cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc
    64621 aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg
    64681 aagttcagca ccaccagata agtcgtcacc aggctggcga acagcaaggt gacgatgagc
    64741 aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagagc
    64801 cgtccggtac aaagacgtag cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg
    64861 gatcttcttc taccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg
    64921 cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg
    64981 gatggctgac cagtgccttc agtaccgcaa actcaccgct ggtgagcggc atcggctcat
    65041 cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg
    65101 cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc accgcacgaa
    65161 tacgggccag cagttcacgc gggttaaacg gttttggaat gtagtcgtca gcgccaatct
    65221 ccaggcctac gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg
    65281 ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta
    65341 aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat
    65401 tagcgacgct tcgaacctgg aagccttgtt cggtgagata acgttccagc agcgcacgca
    65461 ggcgcatgtc gtcatcgacc accagaatct tgtagttctc ttgcattgtt tgtactccca
    65521 aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta
    65581 aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat
    65641 acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg
    65701 cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa
    65761 gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc
    65821 acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag
    65881 tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcctg
    65941 gaaaatctca aaatcgtcga ttactcccct cagcaggaag gcaaagtctt ttttggcgcg
    66001 tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt cggctacgat
    66061 gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg
    66121 aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc gaactggtat
    66181 gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg
    66241 gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact
    66301 caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg
    66361 gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca
    66421 cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg
    66481 cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa atccatttcc
    66541 gagcaaggca aactcaccga tgatctggcg aaggccatca acgccaccct aagcaaaacc
    66601 gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc
    66661 attgaagcag ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc acacacgcca
    66721 gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg tggcagatac caaagccgcg
    66781 ctggatggcg cgcgctatat cctgatggaa cggtttgccg aagatgccac gctgctggcg
    66841 aaagtgcgtg attatctgtg gaagaacgcg catttggttt ctacggtggt gagcggtaaa
    66901 gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg
    66961 ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact ccagctttcg
    67021 ctgaatgccg atccgcagtt cgatgagccg cccaaagaga gctattgcga gcaaatcatc
    67081 atggatcacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg caaaagcgta
    67141 gtgagctgga cctggcgcat caaggtgctg atgcatctgg aaaccgaact gatgggtacc
    67201 gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg
    67261 ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtacc
    67321 ggggtaaaag tggcggtggt cgatgccact ggcaaactgg tggcgaccga taccatttac
    67381 ccgcacaccg gacaagccgc aaaagcagcg atgaccgttg ctgccttgtg tgaaaaacat
    67441 aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaactga acgtttctat
    67501 ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga aagtgatcgt cagcgaagct
    67561 ggcgcgtcgg tttactcagc ttccgagctg gcagcgcagg agttcccgga tctcgacgtt
    67621 tcgctgcgtg gtgcggtatc tatcgcccgc cgtttgcagg atccgctggc ggagctggtg
    67681 aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa
    67741 ctggcccgca aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc
    67801 aacaccgctt ctgttccgct attaacccgc gtggcgggcc tgacgcgcat gatggcgcaa
    67861 aacatcgtgg cctggcgcga tgagaacggc cagttccaga accgtcagca actgctgaaa
    67921 gtcagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcctgcg cattaaccac
    67981 ggtgataacc cgctggacgc ctctaccgtt catccggaag cctatccggt ggtggaacgc
    68041 attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt
    68101 aacctgaaag cgtctgactt tactgatgaa aaattcggtg tgccgacagt aactgacatc
    68161 atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt
    68221 gccgatggcg tcgagacaat gaacgacctg caaccgggta tgatcctcga aggcgcagtg
    68281 accaacgtca ccaactttgg cgcgtttgtc gatattggcg tgcatcagga cggcctggtt
    68341 cacatctctt cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc
    68401 gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgact
    68461 atgcgtctgg acgagcagcc tggcgaaacc aactctcgtc gcggcggcgg taatgaacgc
    68521 ccgcaaaaca accgcccggc agccaaacca cgcggtcgtg aagcgcagcc tgccggtaat
    68581 agcgcgatga tggatgcgct ggcggcagcg atgggtaaaa aacgttaaac gcccggactg
    68641 gcccctaaac ctgcgcgata aggcgttcac gccgcatctg gcaacgaacg ctttgcctga
    68701 cataaaagtg ccggagaata tctccggcac ttttattcca cagccaaact cacaatatac
    68761 tccgttaata tttatcactt cattaacaac tgaaacctta attaaacatt agccagtccg
    68821 ggtaactcac tattcgaatt atattttcgc tgcgatataa ccttgagcca catcaacatt
    68881 gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct gatagaaacc
    68941 attctcatta tcattgtgtt gttgattatt taatctctcc tttgttggca aatcatctgg
    69001 tctcatgtcg cggtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg
    69061 cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca
    69121 gcccggcata tcgccaaaaa ctgctttctc taggcatgtt acctggctcc tcttttgatg
    69181 tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg
    69241 tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg
    69301 ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc tggcaagaca
    69361 accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc
    69421 gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg
    69481 cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc
    69541 tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga tgcgtctaac
    69601 cttgagcgta acctgtacct gacgctgcaa ctgctggaac tcggcattcc ctgcatcgtg
    69661 gcgctgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctcta
    69721 tcggcacgtc ttggctgccc ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa
    69781 gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact ggtgcattac
    69841 gcacagccgc tactcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg
    69901 ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc
    69961 tacgccggtg aagcgtcgca gcatctggat gccaccctcg cccgtctgcg taatgaaatg
    70021 gatgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat
    70081 gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc
    70141 gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcctg
    70201 ctggctatca acatcggcgg ggcgttacag ccgctgttta acgtcggctc cgtggcgcta
    70261 tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg gctgactatc
    70321 ttcctcgccc agggcctggg tggcggtatt aacaccgtgc tgccactggt gccgcagatc
    70381 ggcatgatgt acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcagcg
    70441 tttgtgatgg accgcctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg
    70501 atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg
    70561 cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct
    70621 atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgccctggc ggttttctcg
    70681 ctgtatatgc tcggtatcgt gatggcggtg ctgactggcc tgatgctcaa gtacaccatc
    70741 atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accacacgtt
    70801 aaaagcctga ttatccagac ctggcagcgt ctgaagggct tcgttctgcg tgctggtaaa
    70861 gtgattatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa
    70921 atcgtcgata acatcaacga ctcggcgctg gcttccgtca gccgggtgat aacccctgtc
    70981 ttcaagccga ttggcgtaca tgaagataac tggcaggcaa cggttggcct gtttacgggc
    71041 gcaatggcaa aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt
    71101 caggacgaag agttcaatcc ggcggaattc aacctcggtg aagagctgtt cagtgcggta
    71161 gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt
    71221 gaagctagca aaggcaacgg tgaaatgggt accggggcga tgggcgtgat ggatcagaaa
    71281 ttcggtagcg cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccatgt
    71341 atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatc
    71401 ctgtgggggc tgaatatcgc ttactcactg gcaacattgt tctatcaggt cgccagctac
    71461 agtcagcacc caacttacag cctggtgtgc attctggcgg taatcctgtt taacatcgta
    71521 gctatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc
    71581 aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta atggcttcac
    71641 ttattcaggt acgtgatttg ctggcgttac ggggccgtat ggaagcgacc cagataagcc
    71701 agacattgaa cactccgcag ccaatgatta acgccatgct gcaacagctg gaaagcatgg
    71761 gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt tgtaaaagct
    71821 gcccggaagg caaagcctgt ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg
    71881 cctgttttga aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat
    71941 aaaaatgcaa ttcttgcagc gcaacgtaag ttcctctgaa agcgtctcgc agggatgaaa
    72001 actcgctaat gcacagatgt ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga
    72061 aggaacgcca tgagtaaaaa gcagagttcc accccacatg atgcgctgtt caaactcttt
    72121 ttacgccaac cggagacggc acgcgatttt cttgcgtttc atttaccggc acccattcac
    72181 gcgctctgtg atatgaaaac cctcaagctg gagtcgagga gctttattga tgacgatctg
    72241 cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc aggatacatc
    72301 tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg catgatgcgt
    72361 tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt gccgatggtg
    72421 gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct gtgttggctg
    72481 gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt tccgctgatt
    72541 gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc gctgctggag
    72601 ttaattcaaa aacatattcg tcaacgcgat ctgatggggc tggtagagca aatggcctgc
    72661 ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa ttacatactg
    72721 caaactggcg acgctgtacg ttttaacgat tttatcgacg gcgttgccga acgttcaccg
    72781 aaacacaagg agagtttaat gactattgcg gaaagattgc ggcaggaggg ggaacaatcc
    72841 aaagccctgc atatagccaa aataatgctt gaatccggag tccctcttgc agacatcatg
    72901 cgatttaccg ggctgtcaga agaagagttg gctgcggcga gtagactggc cccctgaatc
    72961 tccagacaac cagtatcact taa
//
LOCUS       DAZPFP010000002        70712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPFP010000002 DAZPFP010000000
VERSION     DAZPFP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.002"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHUR4_000074"
     CDS             <1..795
                     /locus_tag="ACHUR4_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1453840.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHUR4_000075"
     CDS             806..1897
                     /locus_tag="ACHUR4_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1453841.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUR4_000076"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUR4_000076"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1453842.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUR4_000077"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUR4_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1453843.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHUR4_000078"
     CDS             4859..5254
                     /locus_tag="ACHUR4_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1453844.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUR4_000079"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUR4_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1453845.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUR4_000080"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUR4_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1453846.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHUR4_000081"
     CDS             complement(6563..7603)
                     /locus_tag="ACHUR4_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1453847.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHUR4_000082"
     CDS             complement(7676..8311)
                     /locus_tag="ACHUR4_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1453848.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUR4_000083"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUR4_000083"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1453849.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHUR4_000084"
     CDS             complement(8937..9380)
                     /locus_tag="ACHUR4_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1453850.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUR4_000085"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUR4_000085"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1453851.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHUR4_000086"
     CDS             complement(9720..10223)
                     /locus_tag="ACHUR4_000086"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1453852.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHUR4_000087"
     CDS             complement(10217..10741)
                     /locus_tag="ACHUR4_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1453853.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHUR4_000088"
     CDS             10950..11945
                     /locus_tag="ACHUR4_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1453854.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHUR4_000089"
     CDS             11954..12832
                     /locus_tag="ACHUR4_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1453855.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHUR4_000090"
     CDS             12913..13920
                     /locus_tag="ACHUR4_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1453856.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUR4_000091"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUR4_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1453857.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUR4_000092"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUR4_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1453858.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUR4_000093"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUR4_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1453859.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUR4_000094"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUR4_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1453860.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUR4_000095"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUR4_000095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1453861.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUR4_000096"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUR4_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1453862.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUR4_000097"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUR4_000097"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1453863.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUR4_000098"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUR4_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1453864.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUR4_000099"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUR4_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1453865.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUR4_000100"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUR4_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1453866.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUR4_000101"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUR4_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1453867.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHUR4_000102"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHUR4_000102"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHUR4_000103"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHUR4_000103"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1453868.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHUR4_000104"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHUR4_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHUR4_000105"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHUR4_000105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUR4_000106"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUR4_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1453869.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUR4_000107"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUR4_000107"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1453870.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUR4_000108"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUR4_000108"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1453871.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUR4_000109"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUR4_000109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1453872.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUR4_000110"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUR4_000110"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1453873.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUR4_000111"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUR4_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1453874.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUR4_000112"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUR4_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1453875.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUR4_000113"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUR4_000113"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1453876.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHUR4_000114"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHUR4_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUR4_000115"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUR4_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1453877.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHUR4_000116"
     CDS             complement(41212..42177)
                     /locus_tag="ACHUR4_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1453878.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUR4_000117"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUR4_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1453879.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUR4_000118"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUR4_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1453880.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUR4_000119"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUR4_000119"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1453881.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUR4_000120"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUR4_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1453882.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUR4_000121"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUR4_000121"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1453883.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUR4_000122"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUR4_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1453884.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUR4_000123"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUR4_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1453885.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUR4_000124"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUR4_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1453886.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUR4_000125"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUR4_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1453887.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUR4_000126"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUR4_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1453888.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUR4_000127"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUR4_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1453889.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHUR4_000128"
     CDS             49715..50680
                     /locus_tag="ACHUR4_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1453890.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUR4_000129"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUR4_000129"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1453891.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUR4_000130"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUR4_000130"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1453892.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUR4_000131"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUR4_000131"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1453893.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUR4_000132"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUR4_000132"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1453894.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUR4_000133"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUR4_000133"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1453895.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUR4_000134"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUR4_000134"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1453896.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUR4_000135"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUR4_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1453897.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUR4_000136"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUR4_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1453898.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUR4_000137"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUR4_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1453899.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHUR4_000138"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHUR4_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1453900.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUR4_000139"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUR4_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1453901.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUR4_000140"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUR4_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUR4_000141"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUR4_000141"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1453902.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUR4_000142"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUR4_000142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1453903.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHUR4_000143"
     CDS             complement(62547..63476)
                     /locus_tag="ACHUR4_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1453904.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUR4_000144"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUR4_000144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1453905.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUR4_000145"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUR4_000145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1453906.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHUR4_000146"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHUR4_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17316 a        19176 c        17559 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZPFP010000003        65586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPFP010000003 DAZPFP010000000
VERSION     DAZPFP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.003"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(17..496)
                     /gene="folK"
                     /locus_tag="ACHUR4_000147"
     CDS             complement(17..496)
                     /gene="folK"
                     /locus_tag="ACHUR4_000147"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1453907.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(493..1890)
                     /gene="pcnB"
                     /locus_tag="ACHUR4_000148"
     CDS             complement(493..1890)
                     /gene="pcnB"
                     /locus_tag="ACHUR4_000148"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1453908.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1950..2846)
                     /gene="gluQRS"
                     /locus_tag="ACHUR4_000149"
     CDS             complement(1950..2846)
                     /gene="gluQRS"
                     /locus_tag="ACHUR4_000149"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1453909.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2913..3368)
                     /gene="dksA"
                     /locus_tag="ACHUR4_000150"
     CDS             complement(2913..3368)
                     /gene="dksA"
                     /locus_tag="ACHUR4_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1453910.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3546..4250)
                     /gene="sfsA"
                     /locus_tag="ACHUR4_000151"
     CDS             complement(3546..4250)
                     /gene="sfsA"
                     /locus_tag="ACHUR4_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1453911.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4265..4795)
                     /gene="thpR"
                     /locus_tag="ACHUR4_000152"
     CDS             complement(4265..4795)
                     /gene="thpR"
                     /locus_tag="ACHUR4_000152"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1453912.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4869..7298
                     /gene="hrpB"
                     /locus_tag="ACHUR4_000153"
     CDS             4869..7298
                     /gene="hrpB"
                     /locus_tag="ACHUR4_000153"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1453913.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7494..10028
                     /gene="mrcB"
                     /locus_tag="ACHUR4_000154"
     CDS             7494..10028
                     /gene="mrcB"
                     /locus_tag="ACHUR4_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1453914.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10248..12491
                     /gene="fhuA"
                     /locus_tag="ACHUR4_000155"
     CDS             10248..12491
                     /gene="fhuA"
                     /locus_tag="ACHUR4_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1453915.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12542..13339
                     /gene="fhuC"
                     /locus_tag="ACHUR4_000156"
     CDS             12542..13339
                     /gene="fhuC"
                     /locus_tag="ACHUR4_000156"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1453916.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13339..14229
                     /gene="fhuD"
                     /locus_tag="ACHUR4_000157"
     CDS             13339..14229
                     /gene="fhuD"
                     /locus_tag="ACHUR4_000157"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1453917.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14226..16208
                     /gene="fhuB"
                     /locus_tag="ACHUR4_000158"
     CDS             14226..16208
                     /gene="fhuB"
                     /locus_tag="ACHUR4_000158"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1453918.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16243..17523)
                     /gene="hemL"
                     /locus_tag="ACHUR4_000159"
     CDS             complement(16243..17523)
                     /gene="hemL"
                     /locus_tag="ACHUR4_000159"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1453919.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17689..17736
                     /gene="yadX"
                     /locus_tag="ACHUR4_000160"
     CDS             17689..17736
                     /gene="yadX"
                     /locus_tag="ACHUR4_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1453920.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17748..19169
                     /gene="clcA"
                     /locus_tag="ACHUR4_000161"
     CDS             17748..19169
                     /gene="clcA"
                     /locus_tag="ACHUR4_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1453921.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19193..19258
                     /gene="yadW"
                     /locus_tag="ACHUR4_000162"
     CDS             19193..19258
                     /gene="yadW"
                     /locus_tag="ACHUR4_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1453922.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19251..19595
                     /gene="erpA"
                     /locus_tag="ACHUR4_000163"
     CDS             19251..19595
                     /gene="erpA"
                     /locus_tag="ACHUR4_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1453923.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19642..20265)
                     /locus_tag="ACHUR4_000164"
     CDS             complement(19642..20265)
                     /locus_tag="ACHUR4_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1453924.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20303..21103)
                     /gene="btuF"
                     /locus_tag="ACHUR4_000165"
     CDS             complement(20303..21103)
                     /gene="btuF"
                     /locus_tag="ACHUR4_000165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1453925.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21096..21794)
                     /gene="mtnN"
                     /locus_tag="ACHUR4_000166"
     CDS             complement(21096..21794)
                     /gene="mtnN"
                     /locus_tag="ACHUR4_000166"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1453926.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21878..23395
                     /gene="dgt"
                     /locus_tag="ACHUR4_000167"
     CDS             21878..23395
                     /gene="dgt"
                     /locus_tag="ACHUR4_000167"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1453927.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23525..24949
                     /gene="degP"
                     /locus_tag="ACHUR4_000168"
     CDS             23525..24949
                     /gene="degP"
                     /locus_tag="ACHUR4_000168"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1453928.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25104..26260
                     /gene="cdaR"
                     /locus_tag="ACHUR4_000169"
                     /pseudo
     CDS             25104..26260
                     /gene="cdaR"
                     /locus_tag="ACHUR4_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26349..26735)
                     /locus_tag="ACHUR4_000170"
     CDS             complement(26349..26735)
                     /locus_tag="ACHUR4_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1453929.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27049..27873)
                     /gene="dapD"
                     /locus_tag="ACHUR4_000171"
     CDS             complement(27049..27873)
                     /gene="dapD"
                     /locus_tag="ACHUR4_000171"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1453930.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27904..30576)
                     /gene="glnD"
                     /locus_tag="ACHUR4_000172"
     CDS             complement(27904..30576)
                     /gene="glnD"
                     /locus_tag="ACHUR4_000172"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1453931.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30638..31432)
                     /gene="map"
                     /locus_tag="ACHUR4_000173"
     CDS             complement(30638..31432)
                     /gene="map"
                     /locus_tag="ACHUR4_000173"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1453932.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31800..32525
                     /gene="rpsB"
                     /locus_tag="ACHUR4_000174"
     CDS             31800..32525
                     /gene="rpsB"
                     /locus_tag="ACHUR4_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1453933.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32783..33634
                     /gene="tsf"
                     /locus_tag="ACHUR4_000175"
     CDS             32783..33634
                     /gene="tsf"
                     /locus_tag="ACHUR4_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1453934.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33781..34506
                     /gene="pyrH"
                     /locus_tag="ACHUR4_000176"
     CDS             33781..34506
                     /gene="pyrH"
                     /locus_tag="ACHUR4_000176"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1453935.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34798..35355
                     /gene="frr"
                     /locus_tag="ACHUR4_000177"
     CDS             34798..35355
                     /gene="frr"
                     /locus_tag="ACHUR4_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1453936.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35447..36643
                     /gene="ispC"
                     /locus_tag="ACHUR4_000178"
     CDS             35447..36643
                     /gene="ispC"
                     /locus_tag="ACHUR4_000178"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1453937.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36832..37590
                     /gene="ispU"
                     /locus_tag="ACHUR4_000179"
                     /gene_synonym="uppS"
     CDS             36832..37590
                     /gene="ispU"
                     /locus_tag="ACHUR4_000179"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1453938.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37603..38460
                     /gene="cdsA"
                     /locus_tag="ACHUR4_000180"
     CDS             37603..38460
                     /gene="cdsA"
                     /locus_tag="ACHUR4_000180"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1453939.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38472..39824
                     /gene="rseP"
                     /locus_tag="ACHUR4_000181"
     CDS             38472..39824
                     /gene="rseP"
                     /locus_tag="ACHUR4_000181"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1453940.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39854..42286
                     /gene="bamA"
                     /locus_tag="ACHUR4_000182"
     CDS             39854..42286
                     /gene="bamA"
                     /locus_tag="ACHUR4_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1453941.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42408..42893
                     /gene="skp"
                     /locus_tag="ACHUR4_000183"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42408..42893
                     /gene="skp"
                     /locus_tag="ACHUR4_000183"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1453942.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42897..43922
                     /gene="lpxD"
                     /locus_tag="ACHUR4_000184"
     CDS             42897..43922
                     /gene="lpxD"
                     /locus_tag="ACHUR4_000184"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1453943.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44027..44482
                     /gene="fabZ"
                     /locus_tag="ACHUR4_000185"
     CDS             44027..44482
                     /gene="fabZ"
                     /locus_tag="ACHUR4_000185"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1453944.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44486..45274
                     /gene="lpxA"
                     /locus_tag="ACHUR4_000186"
     CDS             44486..45274
                     /gene="lpxA"
                     /locus_tag="ACHUR4_000186"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1453945.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45274..46422
                     /gene="lpxB"
                     /locus_tag="ACHUR4_000187"
     CDS             45274..46422
                     /gene="lpxB"
                     /locus_tag="ACHUR4_000187"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1453946.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46419..47015
                     /gene="rnhB"
                     /locus_tag="ACHUR4_000188"
     CDS             46419..47015
                     /gene="rnhB"
                     /locus_tag="ACHUR4_000188"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1453947.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47052..50534
                     /gene="dnaE"
                     /locus_tag="ACHUR4_000189"
     CDS             47052..50534
                     /gene="dnaE"
                     /locus_tag="ACHUR4_000189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1453948.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50547..51506
                     /gene="accA"
                     /locus_tag="ACHUR4_000190"
     CDS             50547..51506
                     /gene="accA"
                     /locus_tag="ACHUR4_000190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1453949.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51605..53746
                     /gene="ldcC"
                     /locus_tag="ACHUR4_000191"
     CDS             51605..53746
                     /gene="ldcC"
                     /locus_tag="ACHUR4_000191"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1453950.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53803..54192
                     /locus_tag="ACHUR4_000192"
     CDS             53803..54192
                     /locus_tag="ACHUR4_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1453951.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54257..55555
                     /gene="tilS"
                     /locus_tag="ACHUR4_000193"
     CDS             54257..55555
                     /gene="tilS"
                     /locus_tag="ACHUR4_000193"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1453952.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55604..55864)
                     /gene="rof"
                     /locus_tag="ACHUR4_000194"
     CDS             complement(55604..55864)
                     /gene="rof"
                     /locus_tag="ACHUR4_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1453953.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55851..56051)
                     /locus_tag="ACHUR4_000195"
     CDS             complement(55851..56051)
                     /locus_tag="ACHUR4_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1453954.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56217..56762
                     /locus_tag="ACHUR4_000196"
     CDS             56217..56762
                     /locus_tag="ACHUR4_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1453955.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56759..57181
                     /gene="arfB"
                     /locus_tag="ACHUR4_000197"
     CDS             56759..57181
                     /gene="arfB"
                     /locus_tag="ACHUR4_000197"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR1453956.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57195..57905
                     /gene="nlpE"
                     /locus_tag="ACHUR4_000198"
                     /gene_synonym="cutF"
     CDS             57195..57905
                     /gene="nlpE"
                     /locus_tag="ACHUR4_000198"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1453957.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58060..58884)
                     /locus_tag="ACHUR4_000199"
     CDS             complement(58060..58884)
                     /locus_tag="ACHUR4_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1453958.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58937..60655)
                     /gene="proS"
                     /locus_tag="ACHUR4_000200"
     CDS             complement(58937..60655)
                     /gene="proS"
                     /locus_tag="ACHUR4_000200"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1453959.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60766..61473)
                     /gene="tsaA"
                     /locus_tag="ACHUR4_000201"
     CDS             complement(60766..61473)
                     /gene="tsaA"
                     /locus_tag="ACHUR4_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1453960.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61470..61874)
                     /gene="rcsF"
                     /locus_tag="ACHUR4_000202"
     CDS             complement(61470..61874)
                     /gene="rcsF"
                     /locus_tag="ACHUR4_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1453961.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61992..62807)
                     /gene="metQ"
                     /locus_tag="ACHUR4_000203"
     CDS             complement(61992..62807)
                     /gene="metQ"
                     /locus_tag="ACHUR4_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1453962.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62847..63500)
                     /locus_tag="ACHUR4_000204"
     CDS             complement(62847..63500)
                     /locus_tag="ACHUR4_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1453963.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63493..64524)
                     /gene="metN"
                     /locus_tag="ACHUR4_000205"
     CDS             complement(63493..64524)
                     /gene="metN"
                     /locus_tag="ACHUR4_000205"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1453964.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64712..65287
                     /gene="gmhB"
                     /locus_tag="ACHUR4_000206"
     CDS             64712..65287
                     /gene="gmhB"
                     /locus_tag="ACHUR4_000206"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1453965.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15497 a        16271 c        18078 g        15740 t
ORIGIN      
        1 tcgtaatata aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt
       61 cacgcaacgt ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca
      121 gcataaatcc acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca
      181 cttcattacc aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt
      241 tacgaacacg accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg
      301 cgggcgaagt ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca
      361 gcggtggggt gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta
      421 atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa
      481 tatacgccac tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt
      541 acgacgacgc ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg
      601 tgcggaaacc tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc
      661 aacttcagct cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag
      721 tttccatgcg cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg
      781 ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc
      841 gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg
      901 tgccgtctcc agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg
      961 catatcgtta tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat
     1021 cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag
     1081 atgatattca cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag
     1141 cgattcttca aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat
     1201 cggttctgcg gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg
     1261 cagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac
     1321 gccgtccttc agatccttca taccaccaac gtaatcacgg acggtaaaat ccgctacgct
     1381 gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa
     1441 aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac
     1501 gttaccttcg tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac
     1561 atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg
     1621 ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc
     1681 gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac
     1741 cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac
     1801 cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt
     1861 gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc
     1921 aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt
     1981 acaattgccg actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt
     2041 tgctcgacgc tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc
     2101 gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa
     2161 agtttagcgc cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag
     2221 ccaaaaagct ggtacagcgg gatttgcctt actgttggtt caatcagatc agccccacgc
     2281 actatttctg taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac
     2341 aacccatcac ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata
     2401 atgcctcgca gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg
     2461 gcgttgtctg gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt
     2521 tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg
     2581 agtgcttcac gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc
     2641 agaccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc
     2701 gggtcgatat cttctatgcg taccagccag cgaccttgcc gggcgcgagc ctgcaaatag
     2761 ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag
     2821 cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc
     2881 cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt
     2941 tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga
     3001 ataccaattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc
     3061 agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg
     3121 ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc
     3181 tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga
     3241 atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga
     3301 tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct
     3361 tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga
     3421 ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca
     3481 actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta
     3541 ctttactaca atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc
     3601 gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat
     3661 ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa
     3721 aaaataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga
     3781 cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat
     3841 ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg
     3901 cgttcggagc cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt
     3961 gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata
     4021 aatgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg
     4081 tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta
     4141 ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt
     4201 ttgtaacgct gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc
     4261 cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa
     4321 acgaggaggc gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg
     4381 ggaatgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat
     4441 tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta
     4501 agccgcgtgg aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg
     4561 cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg
     4621 ccttctcttt ctctgcgctc acttcgccta aaaatgccag agtcaggtgc aaattatcgg
     4681 cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt
     4741 cgcggatttc tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag
     4801 gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg
     4861 agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg
     4921 attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc
     4981 cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc
     5041 gtctggcggc gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg
     5101 ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gctgaatacc cgcctggaag
     5161 tggttaccga aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg
     5221 gattggtgat ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc
     5281 tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta
     5341 cgctggacaa tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag
     5401 ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg
     5461 aagccgttgc ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat
     5521 ttttacctgg cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca
     5581 gtgatgtggt gctctgcccg ctgtatggcg tgttgtcact gaacgatcag cgaaaagcga
     5641 tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca
     5701 gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct
     5761 ttgatccgcg cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga
     5821 cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca
     5881 aagaacaagc agaacgcgcc gcggcgcaaa gtgagccgaa gatcttacaa agcgatcttt
     5941 ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc
     6001 tggatcaacc gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg
     6061 cgctggaggg tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc
     6121 cgcgtttagc ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa
     6181 aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt
     6241 tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg
     6301 tacgcggcgg tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg
     6361 atcgcattgc tcatcgccgt gggcaagatg gacgctatca actggcaaac agcatgggag
     6421 cgatgctcga tgccgacgac gcgctaagcc gccacgaatg gttgatcgca ccgttattat
     6481 tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg
     6541 aattagtaca acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg
     6601 cgcaaggtac gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc
     6661 agccgctggc gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg
     6721 ataaaggttt aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt
     6781 tatgcgccgc aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt
     6841 tggcgacgct ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc
     6901 tgaaatcact cgacatttat caggcactac gcggattact tgattgggga atgcagcaac
     6961 gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc
     7021 gttatcatga agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg
     7081 ccactaatcc gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg
     7141 cccaaaggcc gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg
     7201 aggtgcaaaa ggagatgaaa gggcgttatc ccaaacacgt ctggccggac gacccggcaa
     7261 acacggcacc gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct
     7321 gtgggagagg gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc
     7381 cctcaaggga gaggggaccg atcgagcgca aattttgaga gatatcttct tctgtcttgt
     7441 aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg
     7501 ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg
     7561 taagccgtcg tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg
     7621 aagaaccgat gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac
     7681 gcggctggct atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt
     7741 acggcgttta tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc
     7801 ccgcggcagt ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg
     7861 aaatggtgaa gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg
     7921 gcgaatttac cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg
     7981 acagtaaaga agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca
     8041 tcgtcaatat ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca
     8101 ccatgatctc ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg
     8161 atttgctggt ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa
     8221 tcagtctcta ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc
     8281 agggtgcgag tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt
     8341 cttactggcg taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca
     8401 aagaccgtat tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg
     8461 aaatccgcgg cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa
     8521 gcctcgacca gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt
     8581 ggcgtaaccc gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc
     8641 aacagattat tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc
     8701 cgcgtggtgg ggtgatctct cctcagccag cctttatgca gctggtgcgt caggagttgc
     8761 aggcaaaact gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg
     8821 actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga
     8881 aacagcgtaa gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg
     8941 aagttcgtgc gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc
     9001 aggcgcgtcg ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc
     9061 agccgaaaat ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc
     9121 cgaatggcca ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag
     9181 tgatgctggt ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg
     9241 cgctggggct gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt
     9301 tgcatccggt tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc
     9361 aggcattcca gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg
     9421 taatcgcgga agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc
     9481 cggcgcaggc ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg
     9541 gtcgtcagct tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca
     9601 acaataacgt agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg
     9661 taggccgtga taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt
     9721 atcagcgtta tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata
     9781 ttgcagatat gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct
     9841 tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc
     9901 cgtcaggcaa tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg
     9961 ctcagcaaga gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta
    10021 gtaattaaca tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc
    10081 ccatctaaga tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct
    10141 cagattgcgt accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata
    10201 atcattctcg tttacgttat tattcacttt acatcagaga tataccaatg gcgcgttcca
    10261 aaactgctca gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta
    10321 gcggcatgtc tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta
    10381 ccgctgcacc tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc
    10441 agtcagctac cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg
    10501 ttgtgaccgc cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct
    10561 acacgccggg tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac cacctgatca
    10621 ttcgcggttt tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc
    10681 agggcaactt ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta
    10741 tgcgtggccc ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg
    10801 tcagcaagcg tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca
    10861 gcctgttcca gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt
    10921 atcgcctgac cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc
    10981 gttatgctat tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc
    11041 tttcttactt ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa
    11101 ccgttgagcc gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga
    11161 acaacaccta ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg
    11221 acacctttac tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg
    11281 tttatggtta cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat
    11341 tagcgccagc ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc
    11401 tgcaaaactt ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc
    11461 acaccctgct gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg
    11521 gttacgacga ctctgtgcca ctgttcaatc tgtacaatcc ggtgaatccc gatttcgact
    11581 tcaatgccaa agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa
    11641 cgggcgttta tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc
    11701 gttatgactg ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg
    11761 atgacaaaca gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac
    11821 cttacttcag ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata
    11881 ttttcgcacc gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc
    11941 gtccgattgt agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg
    12001 accctgaggg ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa
    12061 tcgaagcgaa agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg
    12121 atgcggaata caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac
    12181 acatggcttc gctgtgggct gactatacct tctttgacgg tccgctttca ggtctgacgc
    12241 tgggcaccgg tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag
    12301 tgggaagtta tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg
    12361 cgggctccaa cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct
    12421 gctttaacac ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct
    12481 ttcgtttcta atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt
    12541 tatgcaggaa tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg
    12601 tgtgcccggg cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac
    12661 cggtctgatt ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca
    12721 tcagcctccg tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag
    12781 caaagcgttt gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat
    12841 gaccgtgcgt gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt
    12901 tggtgcggca gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct
    12961 ggcgcatcgg ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat
    13021 gctggtggcg caggatagcc gttgtctgtt gctcgacgaa ccgacttcag cgctggatat
    13081 cgcccaccag gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac
    13141 ggtcattgcc gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc
    13201 cctgcgcagt ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac
    13261 cctcgaaatg atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc
    13321 tgtgagtttt gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg
    13381 gcgatggcgc tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac
    13441 cccaatcgta ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc
    13501 gtgccttacg gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg
    13561 ccggactcag tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa
    13621 atgaaaccgt cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca
    13681 cgtattgcgc cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg
    13741 cgtaaatcgc tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat
    13801 ttagcgcaat acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca
    13861 cgcccgttac tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac
    13921 agcttgttcc aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc
    13981 aacttctggg gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat
    14041 gtgctctgtt ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg
    14101 tggcaggcca tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt
    14161 tatggtgcaa cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt
    14221 aaagcgtgag taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg
    14281 tcgctacggc gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc
    14341 aggccgcctg gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt
    14401 tgccgcgtct ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt
    14461 ttcagcaagt actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg
    14521 cgcaactggg gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt
    14581 ttgctgcgct ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga
    14641 aacggctgtc gccggtaacg ctgattttcg cggggttggt agtgagcctt tattgcggcg
    14701 caatcaatca gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga
    14761 gcactggaac gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc
    14821 tgggcggtgt gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg
    14881 atggcgtggc gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc
    14941 tggcgattgt catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt
    15001 tgttcgcgcc gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc
    15061 tggcgtcgtt gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga
    15121 ctcgcgtgtg gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc
    15181 tactgtggct gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc
    15241 gtgtcgcgac tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt
    15301 tgatggctgt ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga
    15361 gcggggcgtt gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt
    15421 ttgcgggcgt catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga
    15481 tggcaagccc ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc
    15541 tgtttctggt accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg
    15601 cggtgacgct gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca
    15661 tgttactggc ggggatggcg ttaagtaccg cgttcaccat gcttttgatg atgttgcagg
    15721 caagtggtga cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg
    15781 cgaccgatgc tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc
    15841 cgctgtgccg ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag
    15901 ggatggcgct gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga
    15961 ccgcgacgat gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc
    16021 ggatgatggg ctttcgacgg acgatgccac acatcgtaat ttcggcgctg gtgggtggtt
    16081 tactgctggt gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg
    16141 cggggctgct gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga
    16201 gccgttaata gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac
    16261 ccgacgtgca gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa
    16321 gcctgcttca aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa
    16381 acgcttaaaa cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc
    16441 gtcggtaaag aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc
    16501 ttcttctgcc gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc
    16561 gtgaatgccc ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg
    16621 gttaccggaa agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac
    16681 atcacgacga ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca
    16741 ggttaaatct ggcactacgg cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc
    16801 ggtcatcact tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc
    16861 tggcaggaac tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat
    16921 aatacaggca atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt
    16981 ataagtacag gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg
    17041 gcctaacgtg agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta
    17101 acaaccttca aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc
    17161 gctcatcgtc gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag
    17221 ttcggtcacc agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc
    17281 acgctcggcg gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat
    17341 cggcccccag gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc
    17401 gccgtccgct ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg
    17461 ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact
    17521 catggagggt tcctgattcg tagaaaaagt gaatggctgc tattccatgt tattcataac
    17581 aagttaaata ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag
    17641 tataaaagtt ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg
    17701 agtaaaatgc cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata
    17761 ctccctcttt agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac
    17821 ttcttgagcg cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc
    17881 ttgtcgggct ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta
    17941 tgggggcgct ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct
    18001 gttcggcggt gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag
    18061 ggggatcggg gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt
    18121 ggcgtgtatt gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggggc
    18181 tagggcgcga aggaccaacc gtgcagatcg gcggcaatat cagtcgcatg gtgcttgata
    18241 ttttccgcct gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg
    18301 ggctggctgc ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc
    18361 gtccgcagtt tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt
    18421 cgaccattat gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt
    18481 ctgatgcgcc gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt
    18541 tcggccctat ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg
    18601 gcggcaatat taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc
    18661 tggggtttgt ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg
    18721 cggggaattt cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct
    18781 tactctgctt ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta
    18841 ctgtgctggg aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg
    18901 aggcggggac gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc
    18961 cgttaacggg gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa
    19021 tgattattac cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat
    19081 actcggcgat tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca
    19141 aggccgcatc agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat
    19201 tattattggg ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg
    19261 acgtagcact gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg
    19321 ctgacgaaga taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg
    19381 gcttccagta tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa
    19441 aacagggcgt tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg
    19501 ttgactacac cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa
    19561 gcacctgcgg ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac
    19621 tatccggcac atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt
    19681 aagatgccaa cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact
    19741 ggctgtttcc agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat
    19801 aatacaggca gttgcgtaga tttctgtacg taaaatcatg gggatttcgc ggaccagtac
    19861 atcacgaatg atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg
    19921 accggcttcc gcattaaagg ctttattcac gctaatgccg acaaacaccg ccagaccaac
    19981 ggcgtccagc accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat
    20041 ggtcagcatg ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac
    20101 cgggccgtga tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc
    20161 cagtaccaga acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccgtagat
    20221 ggcaaatacc gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact
    20281 gagcacctga aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg
    20341 ctgcgaggat aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg
    20401 gaattttgag ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg
    20461 taatgacaat agcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg
    20521 gaacccggct gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa
    20581 tcgactcttt tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt
    20641 taggtttatc agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg
    20701 cttgttcggc cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa
    20761 tttgttcaat gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt
    20821 caacctgccg ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga
    20881 caatgcgttc cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg
    20941 gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt
    21001 cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga
    21061 gaaaagacag ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc
    21121 agtgactcaa ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta
    21181 agatgagact gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg
    21241 aaattgtggc agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg
    21301 aagttgtggc ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca
    21361 atcaggccac gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc
    21421 agtttatcgt cagctttaaa gcctgccgga cagcctggta actgaccgta ttcataacca
    21481 aatgccgtga catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact
    21541 ttcagcgttg gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag
    21601 tgttccaaca gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga
    21661 agcgcaacct cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata
    21721 gtttgacggt tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg
    21781 atgccgattt tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc
    21841 gcttacgggg aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa
    21901 aaaaataaac tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat
    21961 cctgcggatc ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct
    22021 gcaacaaaag acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac
    22081 ccactcgatg gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct
    22141 gaaagagctt aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag
    22201 cattgttgag atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg
    22261 cgaagcagcg ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca
    22321 gcctctgact gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc
    22381 gcttaacgag ctgcggcgca agattcgtca ggacttatgt cattttgagg ggaatgcaca
    22441 aggcattcgt ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg
    22501 tattttaaaa tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta
    22561 tttaatgaaa aagccgggtt attatctttc tgaagaagcc tatattgccc gcttgcgtaa
    22621 agaacttaat ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga
    22681 cgacatctcc tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt
    22741 tgagcagctt tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt
    22801 ttcgctggtg gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac
    22861 ggaagatcag ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc
    22921 ggcacaacga tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt
    22981 attggaagat gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa
    23041 acatgtgttt agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag
    23101 cggattatta gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact
    23161 ggtagaaaaa gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc
    23221 gacgcgccat cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga
    23281 gtttccgcta tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat
    23341 gaccgacctc tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagt
    23401 cttttgtaaa gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg
    23461 ggctataaaa cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa
    23521 atacatgaaa aaaaacacat tagcactgag tgcactggct ctgagtttag gtttggcgtt
    23581 atctccgctc tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc
    23641 aagccttgca ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg
    23701 tagcacaacc gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga
    23761 ttctccgttc tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca
    23821 gggcggtaat ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat
    23881 tgatgccgat aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat
    23941 taaagtccag ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg
    24001 ctctgatatc gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc
    24061 ggattctgat gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct
    24121 gggcgagacg gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga
    24181 aaactacgaa aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc
    24241 gctggttaac ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg
    24301 cggcaacatc ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca
    24361 gatggtggaa tacggccagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa
    24421 ttccgaactg gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt
    24481 tctgcctaat tcctccgctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact
    24541 gaacggtaag ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt
    24601 gggcagcaaa ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga
    24661 actgcagcag agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg
    24721 cgctgagatg agcaacaaag gcaaagatca gggcgtggta gtgaacaacg tgaagacggg
    24781 cactccggct gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca
    24841 ggcagtgaaa aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc
    24901 actgaacatt cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac
    24961 cccttcctga atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat
    25021 acatcttcat catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct
    25081 aagccgtgca taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg
    25141 atatcgtggc acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg
    25201 ggcgaattat cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc
    25261 tggtactttc acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg
    25321 gtgtgcggca ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg
    25381 gcctgacagg tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg
    25441 aaatgatgct ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg
    25501 aagaactggt gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc
    25561 gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag
    25621 cggtcagctt ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac
    25681 tacgcccgag cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa
    25741 accggcgttg aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca
    25801 actgattacc cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta
    25861 ttttaccggt cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt
    25921 gggtaaacag cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt
    25981 gttactcgac agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg
    26041 gctgaaagcg atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca
    26101 caatgtgcaa ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta
    26161 tcggcttaat cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt
    26221 gctgctgtat gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg
    26281 cggcgctggc tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca
    26341 attcgggatt atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg
    26401 gttacgactg actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg
    26461 ctgcggtcac gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg
    26521 atttcctgga cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga
    26581 cgcggatatt taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag
    26641 attttcagaa tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga
    26701 ttggtaatac ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta
    26761 aattgtgctt attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc
    26821 ctttttcttc cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg
    26881 cgatattcca tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc
    26941 gaaacaagat gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc
    27001 tgagccagaa agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt
    27061 acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat
    27121 aaccgcacag tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac
    27181 agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt
    27241 gctctgacca atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc
    27301 aaccacttca gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg
    27361 cagcggctcc agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat
    27421 ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc
    27481 gccgatgtta acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg
    27541 taccgccgct ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc
    27601 ggcgaatttc atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt
    27661 atcattaata cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg
    27721 accgtcaatt ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt
    27781 taccgcttcg cgggtaacgg tgtctgcatt ggctggcgtg atctcggcac ggcgttcaaa
    27841 agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac
    27901 acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg
    27961 caactcgtta ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg
    28021 ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt
    28081 cccgactcgc gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg
    28141 gtcggtatgg gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc
    28201 gggttggcga cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc
    28261 aaaccgaata acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat
    28321 aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa
    28381 attgcggcgg tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca
    28441 gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct
    28501 taaatcatgc tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac
    28561 aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc
    28621 catgcgcagt agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg
    28681 cgtgttttgc attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag
    28741 actttgcttc cagctattcc acagtgtttc gttggtggcg caaatgtcag ccactgtcag
    28801 gcataccaga tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac
    28861 ttcagggtcc tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac
    28921 cagccaggca accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac
    28981 tacatcctga gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa
    29041 cagcgcggcg atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa
    29101 cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat
    29161 agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg
    29221 cgaccattgc ggcatatacg cgccgagcac gctatggcga tgcattggca atagtccgcg
    29281 ccgcaccgct ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt
    29341 acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta
    29401 aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg
    29461 ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc
    29521 atcgtcgagt ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag
    29581 ttgcagcagc atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat
    29641 catccgctcg accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag
    29701 ttggcgatcg aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg
    29761 aatacgccac agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa
    29821 gccaaacccg accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg
    29881 cagagtgtga atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta
    29941 gctggtgcca tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa
    30001 cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc
    30061 aacatcgcca attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa
    30121 cccttccaac atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc
    30181 ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg
    30241 gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc
    30301 gacggcaacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg
    30361 caggagctgg tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt
    30421 gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac
    30481 ggttaattca tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag
    30541 agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat
    30601 tatcgcatta aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg
    30661 ccgggatggt gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa
    30721 tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt
    30781 tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc
    30841 ccggtttcag cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat
    30901 ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt
    30961 cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc
    31021 gtagcgccag gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg
    31081 gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga
    31141 catcaatgtt aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc
    31201 acaccacttc attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg
    31261 cagaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg
    31321 tgctgacgcc cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc
    31381 cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg
    31441 tccatcagcg tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc
    31501 gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata
    31561 gaaaattcga cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt
    31621 tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg
    31681 acaatctcac tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg
    31741 ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag gttttaatca
    31801 tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc
    31861 gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca
    31921 tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg
    31981 tttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga
    32041 aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc
    32101 tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt
    32161 ctcaggacgg tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc
    32221 tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc
    32281 tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta
    32341 ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc
    32401 cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg
    32461 tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg
    32521 agtaataagg cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg
    32581 agaattcccg ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc
    32641 tgcggcgcga aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag
    32701 ggggcctgca tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga
    32761 tcacatctcc gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg
    32821 tgagcgtact ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga
    32881 catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc
    32941 aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat
    33001 tctggaagtt aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc
    33061 agacaaagtt ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc
    33121 acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg
    33181 ccgcgttgct gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg
    33241 cgttctggtt gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt
    33301 tgctgcaagc aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa
    33361 agaataccag gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga
    33421 gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc
    33481 gttcgttatg gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt
    33541 gactggcttc atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc
    33601 agcagaagtt gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc
    33661 ggcttctttt tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag
    33721 cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac
    33781 atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct
    33841 ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa
    33901 atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg
    33961 ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg
    34021 atgctggcga ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg
    34081 aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca
    34141 gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac
    34201 ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg
    34261 gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc
    34321 gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct gaaagtcatg
    34381 gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg
    34441 aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg
    34501 gaataattcc cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta
    34561 agtctggtta taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc
    34621 ggagcgtaca aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa
    34681 atagcctaat aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca
    34741 cttccactgt gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg
    34801 attagcgata tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa
    34861 acccaaatca gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc
    34921 gtggaatatt acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat
    34981 tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg
    35041 attatggcct ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg
    35101 ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca
    35161 gaacaagcgc gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca
    35221 ctgttgaaag ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag
    35281 aaactgactg atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa
    35341 ctgatgcagt tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg
    35401 gctggcggcg ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac
    35461 cattctgggc tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc
    35521 cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca
    35581 gtgcctggaa ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct
    35641 taaaacgatg ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc
    35701 ttgcgatatg gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc
    35761 tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa
    35821 agaatcactg gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca
    35881 attgttaccg gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca
    35941 gcataatctg ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg
    36001 gtctggtggc cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca
    36061 agcttgccgt catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat
    36121 gatgaacaaa ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca
    36181 gatggaagtg ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg
    36241 cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc
    36301 atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt
    36361 gacatttgcc gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt
    36421 cgaacaaggc caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc
    36481 ttttcttgcg caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga
    36541 aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc
    36601 gagtgaagtc gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag
    36661 agagtcgcgc tatttgttag cgtagggctt cagtgatata gtctgcgcca tctgatcgta
    36721 agtagttggc tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg
    36781 cttcagtatt cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct
    36841 gctactcaac cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt
    36901 atggacggca atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa
    36961 gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg
    37021 ttaacgctgt atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg
    37081 ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac
    37141 gtgcgtctgc gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt
    37201 cgtaaatctg aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac
    37261 tacggtggac gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa
    37321 ggaaacctgc aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat
    37381 gaactggccc ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt
    37441 ttgctttggc aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc
    37501 gatgaacaag actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc
    37561 ggcaccgagc ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg
    37621 atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg
    37681 gggttcgcca ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt
    37741 agcggtttta ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg
    37801 gcgctgatgc tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa
    37861 atctcacttt gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac
    37921 ccaggttccg cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta
    37981 accattgttc ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat
    38041 cattacagtg gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc
    38101 ggcgcatata tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg
    38161 ggtaaaacct ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt
    38221 tatggcatgt gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc
    38281 gcagcgttag cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga
    38341 attaaggaca gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc
    38401 ctgacggctg cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa
    38461 cggaaggtaa tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg
    38521 tacttatcac cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg
    38581 ttgagcgttt ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca
    38641 ccgaatatgt tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg
    38701 cagaaccggt cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac
    38761 gagcggcgat tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact
    38821 ggctggtttt tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca
    38881 attcgatagc tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta
    38941 tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa
    39001 gcaccaccat tacagtagcg ccatttgaca gcgaccaacg gcgggatgta aagctcgatt
    39061 tacgtcactg ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc
    39121 ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca
    39181 aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt
    39241 gggtgacctt tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg
    39301 aaaggcaggg gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta
    39361 aagcgattgg ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag
    39421 ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc
    39481 tgatgaagct gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca
    39541 acctcagtgg gccgatctct atcgccaagg gggctgggat gacagcggaa ctcggggtag
    39601 tttattacct gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc
    39661 cgttgcccgt acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg
    39721 gaccggtatc cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc
    39781 tgttaatggg gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga
    39841 acgcataata acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc
    39901 caccgtatac ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg
    39961 tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga
    40021 tgaagatatc agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg
    40081 cgtccttcgt gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag
    40141 cattactttc tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc
    40201 ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg
    40261 tctggaagac ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac
    40321 cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg tgtcagctga
    40381 aatccagcaa attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca
    40441 tttccaactg cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa
    40501 acagaaactg gcgggcgatc ttgaaaccct gcgcagctac tatctggacc gcggttatgc
    40561 ccgtttcaac atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt
    40621 cacggtgaac atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa
    40681 ccttgccggg cactccgctg aaattgagca actgactaag atcgagccgg gcgagctgta
    40741 taacggcact aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg
    40801 ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa
    40861 attacgcgtg aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg
    40921 taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg
    40981 gttggggagc gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga
    41041 aactgtcgat accgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta
    41101 caaggtgaaa gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag
    41161 tggcgtgagc ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt
    41221 tggtatcaac gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc
    41281 gtacttcacc gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc
    41341 agatgacgcc gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg
    41401 cttcccgatt aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct
    41461 gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc
    41521 gagcacctct gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg
    41581 gacctataac aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac
    41641 cggtaaagtg accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc
    41701 gacttatgtg ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg
    41761 ttatggtgat ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg
    41821 ttccagcacc gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc
    41881 gcatcaggcc agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc
    41941 gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct
    42001 cgagttcatc accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc
    42061 attcttctgg gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg
    42121 atatccggac tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg
    42181 gatgtcccca ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg
    42241 agacaaggca gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa
    42301 ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg
    42361 caaagaactg caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt
    42421 tattagctgc aggtcttggt ttagcactgg caacttctgc tcaggcggct gacaaaattg
    42481 caatcgtcaa tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca
    42541 cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc
    42601 aggctaaaat gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa
    42661 aagacgtgat ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc
    42721 gcgcacgtcg ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga
    42781 aatccgttgc caacagccag gatatcgatc tggttgttga tgcaaacgcc gttgcttaca
    42841 acagcagcga tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc
    42901 cttcaattcg actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg
    42961 atatcgtcat caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca
    43021 tggttaaccc aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga
    43081 cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga
    43141 cttacgcgcg catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac
    43201 ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg
    43261 cggtgattga gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg
    43321 taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc
    43381 atgagatcca gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg
    43441 gctttggtta tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa
    43501 ttattggcga tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg
    43561 acactgttat tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg
    43621 tgattggcga caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg
    43681 gtcgttactg catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca
    43741 aagtgacggt tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt
    43801 cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga
    43861 acattgatga catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact
    43921 aacgttccat cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag
    43981 gccgtgttat tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac
    44041 tcatactctg cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact
    44101 ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc
    44161 tgtcaatgag ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct
    44221 gattctggaa gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact
    44281 ggaaccgggt gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt
    44341 cgtgcctggc gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac
    44401 ccgttttaaa ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg
    44461 tgctcgtagc cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc
    44521 cattgtggaa gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt
    44581 tggaccccat gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg
    44641 tcatactaaa attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa
    44701 ccaggatttg aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat
    44761 tcgcgaaagc gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg
    44821 cagcgacaac ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg
    44881 ctgtattctc gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat
    44941 catcggcggc atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg
    45001 cggctgctcc ggtgtggcgc aggacgtccc tccttatgtt attgcgcagg gtaaccacgc
    45061 aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat
    45121 taccgctatc cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt
    45181 gaaaccggaa attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt
    45241 ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt
    45301 gccctggtcg ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg
    45361 aaagaacgtg tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa
    45421 ggctgcgaag cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc
    45481 ggtcgtctgc gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg
    45541 aagccagatg tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac
    45601 ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga
    45661 cagaaacgcg ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc
    45721 gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct
    45781 gatgccatgc cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac
    45841 gatgcccact gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt
    45901 gccgatttct tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg
    45961 gtgccgctgg tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg
    46021 ccagacctgt cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat
    46081 gcggcactac tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg
    46141 gtggtgggat atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact
    46201 gattatgtct cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag
    46261 gaagagtgtg agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa
    46321 accagccacg cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc
    46381 gatgagcagg cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc
    46441 acacgcagct ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg
    46501 tcaccgctgc ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa
    46561 agctgagcga aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct
    46621 ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca
    46681 tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg
    46741 atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc
    46801 gcgtaccaga aatcagtgcc gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg
    46861 cggcgctgga tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg
    46921 cttttcatct ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg
    46981 ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc
    47041 ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga
    47101 tgatcgatgg cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc
    47161 cagcactggc gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg
    47221 gacatggcgc agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc
    47281 tgggtgatga gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc
    47341 tggcgttgct gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg
    47401 atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg
    47461 gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt
    47521 tttatgaaga acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg
    47581 atgaagaaag ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg
    47641 tggcgactaa cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg
    47701 tcgcgatcca cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc
    47761 agcaatatat gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc
    47821 ttgccaacac cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact
    47881 tcctgccgca gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa
    47941 aagaaggcct ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac
    48001 gccgcccgga atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggct
    48061 tcccgggcta cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac
    48121 cggtagggcc aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca
    48181 ccgacctcga tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg
    48241 tctccatgcc tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac
    48301 acgtggcgga catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg
    48361 cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg
    48421 accgtatctc gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag
    48481 ccgagccgca gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca
    48541 tggcgcgcaa actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta
    48601 tcgcgccgac caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc
    48661 cggtcaccca gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc
    48721 ttggtttgcg tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg
    48781 cgaagaatgg cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct
    48841 tcgacatgct gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga
    48901 aggacctgat caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac
    48961 tgttccgccc tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg
    49021 gtcgtgaaga gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac
    49081 tggagccaac ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc
    49141 tttctggtta taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc
    49201 cggaagagat ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca
    49261 acgccgaact ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta
    49321 acaaatcgca ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc
    49381 actatccggc ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg
    49441 tggtgggcct ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa
    49501 actccggtct ttaccatttc cacgtcaacg acgatggcga aatcgtgtat ggtattggcg
    49561 ccatcaaagg ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg
    49621 gctacttccg cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc
    49681 gggtgctgga aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg
    49741 cgctgatgaa ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag
    49801 ctatcggtca ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat
    49861 cctacgccag ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt
    49921 taggcctgta cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg
    49981 tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacggctg
    50041 cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct
    50101 gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata
    50161 aataccagca attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg
    50221 atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc
    50281 gggaaaaata tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc
    50341 ttttaaaccg actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc
    50401 tctactatca gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct
    50461 ctccgagcga tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac
    50521 tggagtttga ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga
    50581 ttgcagagct ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac
    50641 tggatattaa catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc
    50701 gtaaaatctt cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc
    50761 gtccttatac cctggattac gttcgcctgg catttgatga atttgacgaa ctggctggcg
    50821 accgtgcgta tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc
    50881 cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact
    50941 ttggtatgcc agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac
    51001 gctttaagat gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg
    51061 cagaagagcg tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg
    51121 gcgtaccggt agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg
    51181 gcgtgggcga taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag
    51241 gttgtgcgtc cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg
    51301 gtatcatcgc tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc
    51361 tgggcggcgc tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg
    51421 cggatctggc cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc
    51481 gcctgatgag ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg
    51541 gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga
    51601 acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat
    51661 caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag
    51721 cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg
    51781 ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct
    51841 ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc
    51901 gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca
    51961 gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta
    52021 cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca
    52081 aaaaagcccg gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga
    52141 tgtctctatt tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga
    52201 agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg
    52261 aatatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt
    52321 gatcgaccgc aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc
    52381 agtctggctg aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga
    52441 atttactcgc gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt
    52501 tcatgcggtg atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa
    52561 acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt
    52621 tcatccaatc taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt
    52681 cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca
    52741 cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac
    52801 ctcgcccagt tatcccattg ttgcttcggt taagacggcg gcggcgatgc tgcgtggtaa
    52861 tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt
    52921 ccagcggctg cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt
    52981 ggatgaagcc gaatgcaggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc
    53041 ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga
    53101 cgaacagggc aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga
    53161 cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat
    53221 tggcatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc
    53281 ttacgatctc aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga
    53341 tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa
    53401 acacgatctt tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac
    53461 gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca
    53521 actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt
    53581 gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct
    53641 ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga
    53701 cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc
    53761 ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag
    53821 gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat
    53881 attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg
    53941 gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag
    54001 cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc
    54061 gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat
    54121 ccatacacgc aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg
    54181 tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact
    54241 gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct cacgccagat
    54301 tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg
    54361 gcggacggaa aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc
    54421 caatgccgat gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct
    54481 ggtggtcgaa cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca
    54541 ggcacgttat caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca
    54601 acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc
    54661 cgggctttcg gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt
    54721 gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat
    54781 tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt
    54841 gccgttattg cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact
    54901 ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca
    54961 aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc
    55021 ggcgattatt cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct
    55081 ggtgcgcatc tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt
    55141 gggcgccttt gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg
    55201 gcaaagcgaa accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg
    55261 gctgggaacc gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc
    55321 ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg
    55381 taagctgaag aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc
    55441 actgctgttt tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg
    55501 tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa
    55561 agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca
    55621 ccaccgtacc gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg
    55681 tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg
    55741 cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc
    55801 aggcgagctc aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg
    55861 acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca
    55921 gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc
    55981 ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa
    56041 tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc
    56101 tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc
    56161 gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg
    56221 cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt
    56281 ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc
    56341 tgcgcttgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt
    56401 gtgccgatga tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga
    56461 ttgagctggg gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag
    56521 tggcgctgtt tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca
    56581 aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag
    56641 taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct
    56701 ggttatcgga tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat
    56761 gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg
    56821 tgcgcagggc gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt
    56881 tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca
    56941 tcatttgatc agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga
    57001 actgaaccgc gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga
    57061 acaaaaagcc cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc
    57121 gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata
    57181 aaaagaagga atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct
    57241 ttactctgat gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg
    57301 ccgaactgaa accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag
    57361 gaatcgaaat ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc
    57421 tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg
    57481 acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg
    57541 cgctggagat gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg
    57601 aaccggcaca atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata
    57661 tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata
    57721 acgcagagct ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac
    57781 tgtcagtaga aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag
    57841 tattagcgcc cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc
    57901 agtaaacctg cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg
    57961 cctacagcat ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt
    58021 tacgccgcat ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa
    58081 gcgacccgcc ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc
    58141 tggcttaaac gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca
    58201 gcccgattgc gccagtgtaa gtggatgccc ggatttggca aatgcatcag tgacaaattc
    58261 cgaacaaaac cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag
    58321 gtcgctgacg cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg
    58381 agtcaccata aagggaataa attcgacaat gccgcgatag ttataaccgc tatctttgat
    58441 tttattggca aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa
    58501 gacgaaaagc ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc
    58561 gcctgtcgct tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga
    58621 agtgctgaag acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc
    58681 gccgggtttg agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg
    58741 atgctggaat ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc
    58801 tggctggctg atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg
    58861 gcagtacgcc tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc
    58921 aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag
    58981 tcttaattaa ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga
    59041 ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca
    59101 tcacgcccgg acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt
    59161 cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca
    59221 gaatcgccac ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt
    59281 tctgctcaat cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca
    59341 ggatttggtt acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg
    59401 tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc
    59461 catccgggct tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga
    59521 catcgcgatc ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga
    59581 aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac
    59641 ccagtgaacc cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca
    59701 gcgggcttgc aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc
    59761 gcaccagcag cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct
    59821 taaccgtttt ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt
    59881 tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga
    59941 tagcttctgc cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt
    60001 cttcaccgct ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg
    60061 tgtcggcctg tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg
    60121 catacattgc atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat
    60181 ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga
    60241 acttggtctg gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac
    60301 gaatcaggtc agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac
    60361 ggtcaacaaa acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca
    60421 aatcggctgg ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt
    60481 cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata
    60541 acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct
    60601 cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt
    60661 tacggttcca gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt
    60721 tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag
    60781 cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca
    60841 gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc
    60901 cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg
    60961 cttttcgacc tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata
    61021 gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac
    61081 cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgc ctttatggca
    61141 aacaacctct ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg
    61201 tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg
    61261 ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc
    61321 caggccgcga acggcgtcgg cctggttgta ggaagctatg agatgcagtt cgccgttggc
    61381 gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat
    61441 aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg
    61501 atacatacag cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt
    61561 aatacagcat tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga
    61621 atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga
    61681 tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg
    61741 cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt
    61801 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
    61861 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
    61921 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
    61981 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
    62041 taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
    62101 tcttcacgcg ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg
    62161 tctttcgccg gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg
    62221 atttgcgcgt cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga
    62281 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
    62341 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt
    62401 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
    62461 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
    62521 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
    62581 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
    62641 ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg
    62701 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
    62761 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
    62821 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
    62881 cctgcaaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
    62941 gtcgcgttgt agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
    63001 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
    63061 accagacccg gcaacgcttc cggtaacagc actttacaga cgatctgcat tggcgtggca
    63121 cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
    63181 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca
    63241 atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
    63301 gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
    63361 ataatttgcc ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac
    63421 ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc
    63481 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
    63541 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
    63601 tttaacgcca ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
    63661 acgcgcggtt tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag
    63721 catcggcacg cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat
    63781 atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa
    63841 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc
    63901 acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
    63961 gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct
    64021 ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg caatcgccac
    64081 acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc
    64141 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag
    64201 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
    64261 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa
    64321 ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaagt ttacacaacg
    64381 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
    64441 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
    64501 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca
    64561 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
    64621 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
    64681 cgtcccatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct
    64741 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
    64801 atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt
    64861 ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
    64921 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
    64981 ttattgcccg catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg
    65041 caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
    65101 ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg
    65161 aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga
    65221 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
    65281 gcaatgatta aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag
    65341 ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag
    65401 ccggttcggt tgaagagaaa aaatcctgaa attcagggtt gactctgaaa taggaaagtg
    65461 tgatatacgc cacctcgcga cagtgagctg aaagctgcgt cgcaactgct ctttaacaat
    65521 ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgataa
    65581 atgaat
//
LOCUS       DAZPFP010000004        60419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPFP010000004 DAZPFP010000000
VERSION     DAZPFP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.004"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHUR4_000207"
     CDS             <1..322
                     /locus_tag="ACHUR4_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1453966.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHUR4_000208"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHUR4_000208"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1453967.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHUR4_000209"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHUR4_000209"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1453968.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUR4_000210"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUR4_000210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1453969.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHUR4_000211"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHUR4_000211"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1453970.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHUR4_000212"
     CDS             complement(3726..4955)
                     /locus_tag="ACHUR4_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1453971.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHUR4_000213"
     CDS             complement(5019..5459)
                     /locus_tag="ACHUR4_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1453972.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHUR4_000214"
     CDS             complement(5564..6334)
                     /locus_tag="ACHUR4_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1453973.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHUR4_000215"
     CDS             complement(6331..7257)
                     /locus_tag="ACHUR4_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1453974.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHUR4_000216"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHUR4_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1453975.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUR4_000217"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUR4_000217"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1453976.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUR4_000218"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUR4_000218"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1453977.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUR4_000219"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUR4_000219"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1453978.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHUR4_000220"
     CDS             12964..13311
                     /locus_tag="ACHUR4_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1453979.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHUR4_000221"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHUR4_000221"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1453980.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHUR4_000222"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHUR4_000222"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1453981.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUR4_000223"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUR4_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1453982.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUR4_000224"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUR4_000224"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1453983.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHUR4_000225"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHUR4_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUR4_000226"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUR4_000226"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1453984.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUR4_000227"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUR4_000227"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1453985.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUR4_000228"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUR4_000228"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1453986.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUR4_000229"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUR4_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1453987.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUR4_000230"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUR4_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1453988.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUR4_000231"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUR4_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1453989.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUR4_000232"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUR4_000232"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1453990.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUR4_000233"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUR4_000233"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1453991.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUR4_000234"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUR4_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1453992.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHUR4_000235"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHUR4_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUR4_000236"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUR4_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1453993.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUR4_000237"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUR4_000237"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1453994.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHUR4_000238"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHUR4_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUR4_000239"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUR4_000239"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1453995.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUR4_000240"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUR4_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1453996.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUR4_000241"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUR4_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1453997.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUR4_000242"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUR4_000242"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1453998.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUR4_000243"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUR4_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1453999.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUR4_000244"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUR4_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1454000.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUR4_000245"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUR4_000245"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1454001.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUR4_000246"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUR4_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1454002.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUR4_000247"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUR4_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1454003.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUR4_000248"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUR4_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1454004.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUR4_000249"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUR4_000249"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1454005.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUR4_000250"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUR4_000250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1454006.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUR4_000251"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUR4_000251"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1454007.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUR4_000252"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUR4_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1454008.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUR4_000253"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUR4_000253"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1454009.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUR4_000254"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUR4_000254"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1454010.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUR4_000255"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUR4_000255"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1454011.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUR4_000256"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUR4_000256"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1454012.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUR4_000257"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUR4_000257"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1454013.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUR4_000258"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUR4_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1454014.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUR4_000259"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUR4_000259"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1454015.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUR4_000260"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUR4_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1454016.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14848 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZPFP010000005        59768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPFP010000005 DAZPFP010000000
VERSION     DAZPFP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.005"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUR4_000261"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUR4_000261"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1454017.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUR4_000262"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUR4_000262"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1454018.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUR4_000263"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUR4_000263"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1454019.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUR4_000264"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUR4_000264"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1454020.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUR4_000265"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUR4_000265"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1454021.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUR4_000266"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUR4_000266"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1454022.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUR4_000267"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUR4_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1454023.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUR4_000268"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUR4_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1454024.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUR4_000269"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUR4_000269"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1454025.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUR4_000270"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUR4_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1454026.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUR4_000271"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUR4_000271"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1454027.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="ACHUR4_000272"
     CDS             complement(15666..16703)
                     /locus_tag="ACHUR4_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1454028.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUR4_000273"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUR4_000273"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1454029.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUR4_000274"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUR4_000274"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1454030.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="ACHUR4_000275"
     CDS             19445..19885
                     /locus_tag="ACHUR4_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1454031.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUR4_000276"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUR4_000276"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1454032.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21545)
                     /locus_tag="ACHUR4_000277"
                     /pseudo
     CDS             complement(20612..21545)
                     /locus_tag="ACHUR4_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUR4_000278"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUR4_000278"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1454033.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUR4_000279"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUR4_000279"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1454034.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUR4_000280"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUR4_000280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR1454035.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUR4_000281"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUR4_000281"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1454036.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUR4_000282"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUR4_000282"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1454037.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUR4_000283"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUR4_000283"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1454038.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUR4_000284"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUR4_000284"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1454039.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUR4_000285"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUR4_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1454040.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="ACHUR4_000286"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="ACHUR4_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1454041.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHUR4_000287"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHUR4_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1454042.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHUR4_000288"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHUR4_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1454043.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHUR4_000289"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHUR4_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1454044.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHUR4_000290"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHUR4_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1454045.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHUR4_000291"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHUR4_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1454046.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHUR4_000292"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHUR4_000292"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1454047.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="ACHUR4_000293"
     CDS             37413..37682
                     /locus_tag="ACHUR4_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1454048.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="ACHUR4_000294"
     CDS             complement(37685..38044)
                     /locus_tag="ACHUR4_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1454049.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="ACHUR4_000295"
     CDS             38185..38811
                     /locus_tag="ACHUR4_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1454050.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="ACHUR4_000296"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="ACHUR4_000296"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1454051.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHUR4_000297"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHUR4_000297"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1454052.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="ACHUR4_000298"
     CDS             41651..42316
                     /locus_tag="ACHUR4_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1454053.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHUR4_000299"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHUR4_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1454054.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="ACHUR4_000300"
     CDS             complement(42950..44167)
                     /locus_tag="ACHUR4_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454055.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="ACHUR4_000301"
     CDS             44301..45350
                     /locus_tag="ACHUR4_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1454056.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="ACHUR4_000302"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="ACHUR4_000302"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1454057.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="ACHUR4_000303"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="ACHUR4_000303"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1454058.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="ACHUR4_000304"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="ACHUR4_000304"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1454059.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="ACHUR4_000305"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="ACHUR4_000305"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1454060.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="ACHUR4_000306"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="ACHUR4_000306"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1454061.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="ACHUR4_000307"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="ACHUR4_000307"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1454062.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="ACHUR4_000308"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="ACHUR4_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1454063.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="ACHUR4_000309"
     CDS             complement(51545..51904)
                     /locus_tag="ACHUR4_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1454064.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="ACHUR4_000310"
     CDS             complement(51901..52176)
                     /locus_tag="ACHUR4_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1454065.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="ACHUR4_000311"
     CDS             complement(52235..53359)
                     /locus_tag="ACHUR4_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454066.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHUR4_000312"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHUR4_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1454067.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="ACHUR4_000313"
     CDS             complement(56091..57158)
                     /locus_tag="ACHUR4_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1454068.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHUR4_000314"
     CDS             complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHUR4_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1454069.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57524..59146)
                     /locus_tag="ACHUR4_000315"
     CDS             complement(57524..59146)
                     /locus_tag="ACHUR4_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1454070.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..>59768)
                     /locus_tag="ACHUR4_000316"
     CDS             complement(59408..>59768)
                     /locus_tag="ACHUR4_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1454071.1"
                     /translation="NQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPTPVYE
                     NYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWKIAPN
                     ERGDKKGVPGLSCFQPQ"
BASE COUNT        14121 a        16464 c        15129 g        14054 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcaacgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctgacgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actaaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cacgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcgct tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggcctga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaatat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgatta
//
LOCUS       DAZPFP010000006        59303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPFP010000006 DAZPFP010000000
VERSION     DAZPFP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.006"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            291..1580
                     /gene="sdaC"
                     /locus_tag="ACHUR4_000317"
     CDS             291..1580
                     /gene="sdaC"
                     /locus_tag="ACHUR4_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1454072.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1638..3005
                     /gene="sdaB"
                     /locus_tag="ACHUR4_000318"
     CDS             1638..3005
                     /gene="sdaB"
                     /locus_tag="ACHUR4_000318"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1454073.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3116..3871
                     /gene="xni"
                     /locus_tag="ACHUR4_000319"
     CDS             3116..3871
                     /gene="xni"
                     /locus_tag="ACHUR4_000319"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1454074.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3924..5072)
                     /gene="fucO"
                     /locus_tag="ACHUR4_000320"
     CDS             complement(3924..5072)
                     /gene="fucO"
                     /locus_tag="ACHUR4_000320"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1454075.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5100..5747)
                     /gene="fucA"
                     /locus_tag="ACHUR4_000321"
     CDS             complement(5100..5747)
                     /gene="fucA"
                     /locus_tag="ACHUR4_000321"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1454076.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6294..7610
                     /gene="fucP"
                     /locus_tag="ACHUR4_000322"
     CDS             6294..7610
                     /gene="fucP"
                     /locus_tag="ACHUR4_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1454077.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7643..9418
                     /gene="fucI"
                     /locus_tag="ACHUR4_000323"
     CDS             7643..9418
                     /gene="fucI"
                     /locus_tag="ACHUR4_000323"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1454078.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9527..10946
                     /gene="fucK"
                     /locus_tag="ACHUR4_000324"
                     /pseudo
     CDS             9527..10946
                     /gene="fucK"
                     /locus_tag="ACHUR4_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10948..11370
                     /gene="fucU"
                     /locus_tag="ACHUR4_000325"
     CDS             10948..11370
                     /gene="fucU"
                     /locus_tag="ACHUR4_000325"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1454079.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11428..12159
                     /gene="fucR"
                     /locus_tag="ACHUR4_000326"
     CDS             11428..12159
                     /gene="fucR"
                     /locus_tag="ACHUR4_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1454080.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12203..13303)
                     /gene="rlmM"
                     /locus_tag="ACHUR4_000327"
     CDS             complement(12203..13303)
                     /gene="rlmM"
                     /locus_tag="ACHUR4_000327"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1454081.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13296..13691)
                     /locus_tag="ACHUR4_000328"
     CDS             complement(13296..13691)
                     /locus_tag="ACHUR4_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1454082.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13710..14627)
                     /gene="gcvA"
                     /locus_tag="ACHUR4_000329"
     CDS             complement(13710..14627)
                     /gene="gcvA"
                     /locus_tag="ACHUR4_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1454083.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14978..15205)
                     /locus_tag="ACHUR4_000330"
     CDS             complement(14978..15205)
                     /locus_tag="ACHUR4_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1454084.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15397..16602
                     /gene="csdA"
                     /locus_tag="ACHUR4_000331"
     CDS             15397..16602
                     /gene="csdA"
                     /locus_tag="ACHUR4_000331"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1454085.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16602..17045
                     /gene="csdE"
                     /locus_tag="ACHUR4_000332"
     CDS             16602..17045
                     /gene="csdE"
                     /locus_tag="ACHUR4_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1454086.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17096..17902)
                     /gene="tcdA"
                     /locus_tag="ACHUR4_000333"
     CDS             complement(17096..17902)
                     /gene="tcdA"
                     /locus_tag="ACHUR4_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1454087.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18140..19237)
                     /gene="mltA"
                     /locus_tag="ACHUR4_000334"
     CDS             complement(18140..19237)
                     /gene="mltA"
                     /locus_tag="ACHUR4_000334"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1454088.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19446..19522
                     /locus_tag="ACHUR4_000335"
     tRNA            19446..19522
                     /locus_tag="ACHUR4_000335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19480..19482,aa:Met,seq:cat)
     gene            19556..19632
                     /locus_tag="ACHUR4_000336"
     tRNA            19556..19632
                     /locus_tag="ACHUR4_000336"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19590..19592,aa:Met,seq:cat)
     gene            complement(19707..20960)
                     /gene="amiC"
                     /locus_tag="ACHUR4_000337"
     CDS             complement(19707..20960)
                     /gene="amiC"
                     /locus_tag="ACHUR4_000337"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1454089.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21192..22523
                     /gene="argA"
                     /locus_tag="ACHUR4_000338"
     CDS             21192..22523
                     /gene="argA"
                     /locus_tag="ACHUR4_000338"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1454090.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22585..24411)
                     /gene="recD"
                     /locus_tag="ACHUR4_000339"
     CDS             complement(22585..24411)
                     /gene="recD"
                     /locus_tag="ACHUR4_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1454091.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24411..27953)
                     /gene="recB"
                     /locus_tag="ACHUR4_000340"
     CDS             complement(24411..27953)
                     /gene="recB"
                     /locus_tag="ACHUR4_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1454092.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27946..30834)
                     /gene="ptrA"
                     /locus_tag="ACHUR4_000341"
     CDS             complement(27946..30834)
                     /gene="ptrA"
                     /locus_tag="ACHUR4_000341"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1454093.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31010..34378)
                     /gene="recC"
                     /locus_tag="ACHUR4_000342"
     CDS             complement(31010..34378)
                     /gene="recC"
                     /locus_tag="ACHUR4_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1454094.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34391..34741)
                     /locus_tag="ACHUR4_000343"
     CDS             complement(34391..34741)
                     /locus_tag="ACHUR4_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1454095.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34726..35133)
                     /locus_tag="ACHUR4_000344"
     CDS             complement(34726..35133)
                     /locus_tag="ACHUR4_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1454096.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35130..35693)
                     /locus_tag="ACHUR4_000345"
     CDS             complement(35130..35693)
                     /locus_tag="ACHUR4_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1454097.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35684..36154)
                     /locus_tag="ACHUR4_000346"
                     /pseudo
     CDS             complement(35684..36154)
                     /locus_tag="ACHUR4_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36338..37132)
                     /gene="thyA"
                     /locus_tag="ACHUR4_000347"
     CDS             complement(36338..37132)
                     /gene="thyA"
                     /locus_tag="ACHUR4_000347"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1454098.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37139..38014)
                     /gene="lgt"
                     /locus_tag="ACHUR4_000348"
     CDS             complement(37139..38014)
                     /gene="lgt"
                     /locus_tag="ACHUR4_000348"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1454099.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38165..40411)
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000349"
     CDS             complement(38165..40411)
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000349"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1454100.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40424..40954)
                     /gene="rppH"
                     /locus_tag="ACHUR4_000350"
     CDS             complement(40424..40954)
                     /gene="rppH"
                     /locus_tag="ACHUR4_000350"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1454101.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41307..41453)
                     /gene="ygdT"
                     /locus_tag="ACHUR4_000351"
     CDS             complement(41307..41453)
                     /gene="ygdT"
                     /locus_tag="ACHUR4_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1454102.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41639..42328
                     /gene="mutH"
                     /locus_tag="ACHUR4_000352"
     CDS             41639..42328
                     /gene="mutH"
                     /locus_tag="ACHUR4_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1454103.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42397..43110
                     /locus_tag="ACHUR4_000353"
     CDS             42397..43110
                     /locus_tag="ACHUR4_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1454104.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43248..43466
                     /gene="ygdR"
                     /locus_tag="ACHUR4_000354"
     CDS             43248..43466
                     /gene="ygdR"
                     /locus_tag="ACHUR4_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1454105.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43574..44614
                     /locus_tag="ACHUR4_000355"
     CDS             43574..44614
                     /locus_tag="ACHUR4_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1454106.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44647..45837)
                     /gene="lplT"
                     /locus_tag="ACHUR4_000356"
     CDS             complement(44647..45837)
                     /gene="lplT"
                     /locus_tag="ACHUR4_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1454107.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45830..47989)
                     /gene="aas"
                     /locus_tag="ACHUR4_000357"
     CDS             complement(45830..47989)
                     /gene="aas"
                     /locus_tag="ACHUR4_000357"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1454108.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48575..49606
                     /gene="galR"
                     /locus_tag="ACHUR4_000358"
     CDS             48575..49606
                     /gene="galR"
                     /locus_tag="ACHUR4_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1454109.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49613..50875)
                     /gene="lysA"
                     /locus_tag="ACHUR4_000359"
     CDS             complement(49613..50875)
                     /gene="lysA"
                     /locus_tag="ACHUR4_000359"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1454110.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50997..51932
                     /gene="lysR"
                     /locus_tag="ACHUR4_000360"
     CDS             50997..51932
                     /gene="lysR"
                     /locus_tag="ACHUR4_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1454111.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51919..52611)
                     /gene="ygeA"
                     /locus_tag="ACHUR4_000361"
     CDS             complement(51919..52611)
                     /gene="ygeA"
                     /locus_tag="ACHUR4_000361"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1454112.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52740..54158)
                     /gene="araE"
                     /locus_tag="ACHUR4_000362"
     CDS             complement(52740..54158)
                     /gene="araE"
                     /locus_tag="ACHUR4_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1454113.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54473..55234)
                     /gene="kduD"
                     /locus_tag="ACHUR4_000363"
     CDS             complement(54473..55234)
                     /gene="kduD"
                     /locus_tag="ACHUR4_000363"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1454114.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55264..56100)
                     /gene="kduI"
                     /locus_tag="ACHUR4_000364"
     CDS             complement(55264..56100)
                     /gene="kduI"
                     /locus_tag="ACHUR4_000364"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1454115.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56387..57568)
                     /locus_tag="ACHUR4_000365"
     CDS             complement(56387..57568)
                     /locus_tag="ACHUR4_000365"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1454116.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57823..59052
                     /locus_tag="ACHUR4_000366"
                     /pseudo
     CDS             57823..59052
                     /locus_tag="ACHUR4_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT        14596 a        15953 c        14494 g        14260 t
ORIGIN      
        1 cggaagaagt cagatgtcgt ttaatgggca aatattgccc ttaaattctc ttttactttt
       61 gatttacaga gtaaagcgtt gggataatct atcttccaag cagattattg tatttgggat
      121 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggcg
      181 gtaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttcccg
      241 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
      301 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
      361 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
      421 acgccggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
      481 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
      541 acatcaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attaccctgc
      601 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaataccg
      661 ttgaaagctt catgtctcac cagctgggta tgacgccacc gccgcgtgcg attctgtcgc
      721 tgatcctgat cgtgggtatg atgaccatcg ttcgctttgg tgagcagatg atcgttaaag
      781 cgatgagtat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
      841 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
      901 gaaacggtct gtggatgacc ctgtggctgg caattccggt aatggtgttc tcgttcaacc
      961 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
     1021 aacagaaatg ctcgaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
     1081 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
     1141 agaacatctc gattctgtct tacctggcta accactttaa cgcaccggtt atcgcgtgga
     1201 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
     1261 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
     1321 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
     1381 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
     1441 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaagtaca
     1501 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
     1561 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
     1621 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
     1681 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
     1741 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
     1801 tgactggtaa aggccaccac acagatatcg ccattattat gggcctggcg ggtaacctgc
     1861 cggataccgt ggatatcgat tccatccccg gttttattca ggatgtgaat actcatggtc
     1921 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
     1981 tccatgcaga caacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggcg
     2041 ataaagtcgt ttacagccag acttactact ctattggcgg tggctttatc gttgatgaag
     2101 agcattttgg ccagcaggat agcgcaccgg ttgaagttcc ttatccgtac agttcagcag
     2161 ccgatctgca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
     2221 acgagctggc gctgcacagt aaagaagagc tggaacagca cctggcgaac gtctgggaag
     2281 tcatgcgtgg cggtattgag cgcggtattt ccaccgaagg cgtgttgcct ggcaaactgc
     2341 gcgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagccaggat aaaaccacca
     2401 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
     2461 acgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attatcccgg
     2521 ctgtgctggc gtactacgat aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
     2581 acctgctggt agccagcgcc attggttctc tttataagat gaacgcgtcg atttctggtg
     2641 ccgaagtggg ttgccagggt gaagttggcg tggcgtgctc tatggcggca gctggtctgg
     2701 cagaactgtt aggcgcaagc ccggcgcagg tttgcatcgc agcggaaatc gcgatggagc
     2761 acaacctcgg tctgacttgt gacccggtcg cggggcaggt tcaggtaccg tgcattgagc
     2821 gtaacgccat tgcagcagtg aaagcggtga acgccgcgcg tatggcactg cgccgtacca
     2881 gcgaaccgcg cgtctgcctc gataaagtta tcgaaaccat gtacgaaaca ggtaaagaca
     2941 tgaacgccaa gtaccgcgaa acctctcgcg gcggcctggc gatgaagatc gttgcctgcg
     3001 attaatctct cccagaggcc tcgttttgcg aagcctcttc ctgttttctc aaccagccat
     3061 agcctgttcc ggcatcgaat gttaccctta tcgcctgatc tttaaggggg ttatcgtggc
     3121 tgttcatttg cttattgtcg atgcactgaa tcttatccgt cgcattcatg ccgttcaggg
     3181 ttcgccctgt gtcgaaacct gccagcatgc gctcgatcag ctcattatgc acagccagcc
     3241 aacccacgcg gtcgccgttt ttgatgatga aaaccgcagt agcggctggc gtcatcagcg
     3301 tttaccagat tacaaagcgg atcgaccgcc aatgccggaa gagttgcacg acgagatgcc
     3361 tgcattacgc gccgcctttg agcaacgcgg cgtcccgtgc tggtcagcca gcggcaacga
     3421 agccgatgac ttagccgcca cactggcggt caaagtgaca caggccgggc atcaggcaac
     3481 gattgtttcg acagataaag gctactgtca gttactttca ccgacattac gtattcgtga
     3541 ttacttccag aaacgttggc tggatgcgcc atttatcgat aaagaatttg gcgttcaacc
     3601 gcagcagttg cccgattact ggggacttgc ggggatcagc agttcaaagg taccgggtgt
     3661 tgcgggaatc ggaccaaaaa gcgccacgca gctgctggtc gagtttcaga gtctggaagg
     3721 gatatatgag aatctggatg cggttgccga aaagtggcgc aaaaaattag aaacccataa
     3781 agagatggcg tttctgtgcc gcgatattgc ccgcttacaa accgatttgc atatcgacgg
     3841 caatttacag caattgcggt tggtacggta acggcgagcc ggatacgccg caaatcgtat
     3901 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
     3961 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgagccagt
     4021 gccgaaatgt cttccttgcg tacaccaaca tcacgcaaat gtggcggaat accgacatca
     4081 cggttgaggg caaacaccgc ttcaacagcg gcattacgcg ccccttccag gctcatacct
     4141 tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt aaagtcagcg
     4201 ttatagcgca tgacatgcgg taacaggatg gcgttcgcaa caccgtgtgg agtgttataa
     4261 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaagccc
     4321 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
     4381 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc atcggttagt
     4441 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc aacacccgtc
     4501 gcggctttca gcgccggagg cataccatcc atcatgtcag catcaataaa cgccacctgc
     4561 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc agtgatcacg
     4621 tagttaatgg tcacttctgc cgcagtacct gctgtggtgg ggattgccag aatcggtaca
     4681 ctgggtttat tggtcgggga aagcccttcc aggctacgca catcggcaaa ctccgggttg
     4741 ttgctgataa tgccaattgc tttacaagta tcctgtggag aaccaccacc aatagcgatc
     4801 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
     4861 gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
     4921 gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
     4981 tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg accaaaccat
     5041 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
     5101 tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc aatctcttca
     5161 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgcgccagc
     5221 acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca agcgataagc
     5281 ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac atgttcagaa
     5341 agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc gccagccgcc
     5401 gcaatcatgt agtgaatagc ggggatcgat cggttaagaa tggaaactgc cgtgcaatga
     5461 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc tgccatatgg
     5521 aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc atcaataaag
     5581 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
     5641 tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag gcgggtcatt
     5701 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
     5761 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
     5821 acccaccatc ttcttcctga ttaacaagaa agaaattcac aagcttatat tttgtgacct
     5881 agctcaacta atcacagtaa ataactgcaa gttctctttt tataaccccg ttaaaaatga
     5941 ccgctcttaa aaatatttat caaaacggtc atttttccat ttcccctcgc cagaaatgcc
     6001 cgttttcggc acaaacaatt aatacggtca tctggtttgt gttttttatg atttattttc
     6061 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcaccttcca
     6121 gctaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
     6181 agttcatttc agacaggcaa atattcaccg atatgaagcc cgaactcgct ggttttgcac
     6241 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
     6301 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
     6361 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
     6421 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
     6481 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
     6541 tattgatgaa aaaactcagt tataaagcag ggattattac cggattattt ttatatgcct
     6601 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
     6661 gcctatttat tattgcagcc ggattaggtt gtctggaaac tgccgcaacc ccttttgtta
     6721 cggtattagg gccggaaagc agtagtcact tccgcttaaa tcttgcgcaa acatttaact
     6781 cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct tattttgtct aacgtgccac
     6841 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
     6901 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
     6961 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
     7021 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
     7081 cggtattagc gcaattctgc tatgtcggcg cacaaacggc ctgctggagc tatttgattc
     7141 gctacgctgt agaagaaatt ctaggtatga ctgcaggctt tgccgctaac tatttaaccg
     7201 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
     7261 caccacacaa agtcctggcc gcctacgcat taatcgctat ggcactgtgc ctgatctcag
     7321 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
     7381 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
     7441 gttcgtcctt catcgttatg accattattg gcggcggtat tgtcactccg gtcatgggtt
     7501 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
     7561 cggtcatctt tatctttgcc cgtttccgtt ctcaaacggc aactaactga acatattttc
     7621 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
     7681 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
     7741 ggcgaaagct acggccgcac tgctgaccga gaaactgcgc catgcctgcg gagctgccgt
     7801 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
     7861 aaaattcagc agccagaatg taggcctcac cattacggta acgccttgct ggtgctatgg
     7921 cagtgaaacc atcgacatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
     7981 tgaacgcccc ggcgctgttt acctggctgc ggctctggca gctcacagcc agaaaggcat
     8041 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
     8101 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttggccgtcg ccagcatgaa
     8161 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ctattgttga
     8221 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
     8281 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cactggcctg
     8341 ggctgataaa aacttccgct atggcgaaga tgaaaataac aaacagtatc aacgcaatgc
     8401 cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
     8461 gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg gaagaatcac ttggctacaa
     8521 cgccatcgct gcgggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggtga
     8581 caccgccgaa gcgatcctca acagttcatt tgactggaat ggcgtgcgcg aaccctttgt
     8641 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ctaatgggtc accagctcac
     8701 cggcaccgct caggtatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
     8761 tgtaacgggg cataaactgg acggactggc agaacacggc atcatccatt tgatcaactc
     8821 aggttctgct gcgctggacg gttcctgtaa acaacgcgac agcgaaggta acccgacgat
     8881 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccgaatg
     8941 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
     9001 aggcggcgtc ccgttcacca tgactcgtgt caacatcatc aaaggcctgg gaccggtact
     9061 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
     9121 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcactg gtaaagggcc
     9181 gtttacggat gtgtattcgg taatggcgaa ctggggtgcc aaccacggag ttctgaccat
     9241 cggccacgtt ggcgcagact ttatcaccct tgcctccatg ctgcgtatcc cggtatgtat
     9301 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggctgcgc acggcatgga
     9361 tattgaaggc caggattacc gcgcttgcca gaactacggt ccgttgtaca agcgttaata
     9421 cattacaccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
     9481 gttagccgga taaacaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
     9541 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
     9601 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
     9661 ctggcaccag tggtctttag actgccattt tgcaacgctt tgctgattgc tgtcggcaaa
     9721 tcaatagtga actgactgaa tgccacatcc gcggtatcgc cgtcaccacc tttagtgtgg
     9781 atggcgctct ggtagataag caaggcaatc tgctctatcc gatcattagc tggaaatgtc
     9841 cgcgaacagc agcggttatg gacaatattg aacggttaat ctccgcacag cggttgcagg
     9901 ctatttctgg cgtcggagcc tttagtttca atacgttata taagttggtg tggttgaaag
     9961 aaaatcatcc acaactgctg gaacgcgcgc acgcctggct cttcatttcg tcgctgatta
    10021 accaccgttt aaccagcgaa ttcactactg atatcacgat ggccggaacc agccagatgc
    10081 tggatatcca gcaacgcgat ttcagtccgc aaattttaca agccaccggt attccacgcc
    10141 gactcttccc tcgtctggtg gaagcgggtg aacagattgg tacgctacag aacaacgccg
    10201 cagcaatgct cggcttaccc gttggcatac cggtgatttc cgcaggtcac gatacccagt
    10261 tcgccctttt tggcgctggt gccgaacaaa atgaacccgt gctctcctcc ggtacatggg
    10321 aaattttaat ggttcgcagc gcctaggttg atacttcgct gttaagtcag tacgccggtt
    10381 ccacctgcga actagatagc caggcatggt tgtataaccc aggtatgcaa tggctggcat
    10441 ccggcgtgct ggaatgggtg agaaaactgt tctggacggc tgaaacaccc tggcaaatgt
    10501 tgattgaaga agctcgtctg atcgcacctg gcgcggatgg tgtgaaaatg cagtgtgatt
    10561 tattgtcgtg tcagaacgct ggctggcaag gagtgacgct taataccacg cgggggcatt
    10621 tctatcgcgc ggcgctggaa gggttaactg cgcaattaca gcgcaatcta cagatgctgg
    10681 aaaaaatcgg gcactttaaa gcctatgaat tattgttagt cggtggagga agtcgcaaca
    10741 cattgtggaa tcagattaaa gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg
    10801 ccgaaacgac cgtcgcagga gctgcgctgt tcggttggta tggcgtaggg gaatttaaca
    10861 gcccggaaga agcccgtgca cagattcatt atcagtaccg ttatttctac ccgcaaactg
    10921 aacctgaatt tatagaggaa gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc
    10981 gaactattga aagtgctggc agagatggga catggagatg aaattatttt ttccgatgct
    11041 cactttcccg cccattcgat ggaaccgcag gtgatccgcg ctgatggcct gttggtgagc
    11101 gacttgctcc aggcgattat cccgttattt gagctggaca gttatgcccc gccgctggtg
    11161 atgatggcgg cggtagaggg tgacactctc gatcctgaag tagaacgacg ttaccgtaat
    11221 gcgctttcac tacaagcccc gtgtcctgac atcatccgca tcaatcgttt tgcgttttat
    11281 gaacgggcgc aaaaagcctt tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat
    11341 attcttttaa aaaaaggggt aacaccgtaa tctcataccg gtacgcccgc atgacgcggg
    11401 cggttatcga atgatggggt gaaaaatatg aaagcggcac gccagcaagc gatagtcgac
    11461 ctgctgctga accataccag cctgaccacg gaagctctct ctgaacagct aaaggtcagt
    11521 aaagaaacca ttcgtcgcga tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat
    11581 catggacgcg ctaaatatat ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc
    11641 aggctgaaaa gccattatgc gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt
    11701 gaagaaggga tggtgatagc cttagacgcc agttcaactt gctggtatct ggcacgccag
    11761 ttgcctgaca tcaacattca ggtcttcacc aatagccatc cgatttgcca tgaactcggt
    11821 aaacgcgaac gcattcaact gatcagttcc ggcggcacac ttgagcgcaa atatggctgt
    11881 tacgtcaatc cctcgctgat ttcccaactt aaatcgctgg aaatcgatct gtttattttt
    11941 tcttgtgaag ggatcgatag cagcggcgca ctgtgggact ccaatgcgat caacgctgat
    12001 tacaaatcga tgctattaaa acgtgccgcg caatcgttgt tattgattga taaaagtaaa
    12061 tttaatcgtt caggggaagc ccgcatcggg catctggatg aggtaacgca cattatttct
    12121 gatgagcgcc aggttgcaac ttctttggta acagcctgac ggaaagggta gcaggccgga
    12181 gacgacgccc cggccttgcc tgttatcgct cgtcgcgacg accacccacc gcagcccaga
    12241 tgcggcggac gtgcaccgtc acttcttcgc gatcgtgata caactgccgc gcctgaatct
    12301 gagcatttat gccatgttca tcaagctgtg cctgaatata cgccagattg tgtgatactt
    12361 cttcgtagcg ttttttcatc ggcagtttga gattgaaaat ggtttcacgg caccagccat
    12421 taaccagcca ctgcgccatc aatgccgcaa ctttagccgg tttttcaacc atatcgcata
    12481 ccatccagga gatattgctg cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg
    12541 tcacctgccc ggtatccatc agactttgcg ccatcgggcc gttgtcgacg gaataaaccc
    12601 acatgttgcg cttcaccagt tggtaggtcc agccgccagg gcaagcgcct aaatccaccg
    12661 cccacatccc gttcgccagg cgttcatccc actcatccgc aggaataaac acatgaaatg
    12721 cctcttccag tttgagcgtg gaacgactcg gcgcctctgc cggaaatttc aggcgcggaa
    12781 tgcccatata gaacggcgaa ttattgttgc tgtatgagta accggtatag cagcatcctg
    12841 gtgcaataaa gaatacatgc acaaccggac gcttcggcgt ttcatagttc gccagcaccc
    12901 cagcatcgcg caaggcagcg cgtagcggaa cagtgaactt acggcagaat ttcagcaact
    12961 ctttgctttc gttggtatcg gcaacttcaa cacgcagttc accgcccttc tctactacac
    13021 cctgtaacat gccgacaatg ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt
    13081 cccccaccac aaaccactgg cgggcaaaaa ttaatgaact gaacggcagc tcacggatta
    13141 acttatcgcc atcatcaggt tgataacatt cataaatgac ataacccgca ttctctttca
    13201 cgcgggcaaa accgaaaatt tcccgctggc cggctttatc ggtaatttct gcggcgcact
    13261 ctttttcaaa gcccggacgg cacagcaata caaccttatt catgacttac gcccttgcgc
    13321 tttaaacgga tagcaccaac taacattaac gcccagcccg caaggaagct cacgccgcca
    13381 accggtgtga caaacgccca caaacgcaga tgggacagcg ccaggcaata aaggctgccg
    13441 ctgaacagca ccgtgcctaa cgcgaggaaa acgctactcc agtaaaacca gatactgatg
    13501 cgacgctgca ttgccaccgc cagacctaag atcgccagcg tatgaaacgc ctggtattcg
    13561 agaccggttt ggatccagcc catctcaacg gcccccatgg ttttacttaa cacatgcgca
    13621 ccaaaagcgc ccagtgccac aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa
    13681 cggctggtca tggtcgtacc ctacgtaaat tattgttcat aacgaaagcg gaatttttct
    13741 tgttcagcag cggctttcgc caggatccat tggcgaaagg cggctatttt acccagttct
    13801 gcctggctgt catgacaaac cagataaaaa gcatttttac tgaccagaac atcattaaac
    13861 gggcaaacaa gacgtccggc ctcgatttca gattgcgcca tcacgttatt tgccagcgcc
    13921 actccctgcc cgtgaatagc cgcttgcagc accatggcgc tatgactaaa aattggccct
    13981 tgctgaacgt tgatatgatt aaaccccaac tgtcgggtat atgtctgcca gtcacggcgt
    14041 gacgcatcat gtaataacgt atgtttagcc agatcttccg gggtcttcaa gggtttttcg
    14101 cctgtcagca gtagcggcga acacaccggc aataaatatt cggcgtacag tttttccacc
    14161 cgtagccccg gccagttgcc ccgaccataa aatatcgcca catcaacatc atccgccagc
    14221 ttatcttcct gacgatcaac cgcctggatt cgaacgtcaa ttcccggata agctgaatta
    14281 aagctggaaa gtcgcggaac caaccaatga atggcgaaac tggggagtaa actgaccgtc
    14341 aacgccccct tggcgctacg ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat
    14401 atctctttga tatcgaggaa atagctttgc ccttcctcgg tcagcaggag tgaacgatta
    14461 cggcggcgga acagttttag ccccaaaaaa tcctcaagag acttgatttg atgacttact
    14521 gcggcctggg tcacaaaaag ctcttctgct gcgcgagtaa aacttaaatg gcgtgctgcg
    14581 gcatcaaaaa ctcgtaaggc atttagcggt ggtaatcgtt tagacatggc ttttaaactt
    14641 tgatgttaaa tgaatttaac aattagatca cactgtgtaa cctattagtt tttttaatct
    14701 gagccattat aaattgtccg ttgagcttct accagcaaat acctatagtg gcggcacttc
    14761 ctgagccgga acgaaaagtt ttatcggaat gcgtattctg gtgaactttt ggcttacggt
    14821 tgtgatgttg tgttgttgtg tttgcaattg gtctgcgatt cagaccatgg tagcaaagct
    14881 accttttttc acttcctgta catttaccct gtctgtccat agtgattaat gtagcaccgc
    14941 ctaattgcgg tgcttttttt taccttgcga tcgcgaatta ctgttccagt tcgaccatct
    15001 ctttcacgtc agtacggttg atctgctgtt tgttgccatt agcgtcttta tacgaaatca
    15061 taccgatatc gttatcagtc tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg
    15121 tgtgcatcac atagttcgaa ccggaacagg cgctcagggc aaaagtcagc atacaggcag
    15181 aaataattgc ggcagtcttt ttcatcatct tctccttaaa acgattaata aatcttaata
    15241 gtattgatta acaggctaaa attaacgcct aacactattc agcatatgtt acttacgcgg
    15301 cttcgccagg atatccagat aattctgatg gttagcactc tccttgtatc aaagtgaatt
    15361 ttgcgtcacg atcggtgcat caagccgagg agtaccatga acgtttttaa tcccgcgcag
    15421 tttcgcgccc agtttcccgc actacaggat gcgggcgtct atctcgacag cgccgcgacc
    15481 gcgcttaaac ctgaagccgt ggttgaagcc actcgacagt tttacagcct gagcgccgga
    15541 aacgtccatc gcagccagtt tgccgaagcc caacgcctga ccgcgcgtta tgaagctgca
    15601 cgagagaaag tggcgcaatt actgaatgca ccggatgata aaactatcgt ctggacgcgc
    15661 ggcaccactg aatccatcaa catggtggca caatgctatg cgcgtccgcg tctgcaaccg
    15721 ggcgatgaga ttattgtcag cgtggcagaa caccacgcta acctcgtccc ctggctgatg
    15781 gtcgcccaac aaactagagc caaagtggtg aaattgccgc ttaatgcgca gcgactgccg
    15841 gatgtcgatt tgttgccaga gctgattact ccccgtagtc ggattctggc gttgggtcag
    15901 atgtcgaacg tgactggcgg ttgcccggat ctggcgcgag cgattacctt tgctcattca
    15961 gccgggatgg tggtgatggt tgatggtgct caggggacgg tgcatttccc cgcggatgtt
    16021 cagcaactgg atatcgattt ctatgctttc tctggtcaca aactgtatgg cccgacgggt
    16081 atcggcgtgc tgtatggcaa atcagaactg ctggaagcga tgtcgccctg gctgggtggc
    16141 ggcaaaatga ttcacgaagt gagttttgac ggtttcacca ctcaatctgc gccgtggaaa
    16201 ctggaagcag gaacgccaaa tgtcgctggc gtcataggat taagcgcggc gctggaatgg
    16261 ctggcagatt acgatatcaa ccaggccgaa agctggagcc gtagcttagc aacgcttgca
    16321 gaagaagcgc tggcgaaacg tccaggcttt cgttcattcc gctgccagga ttccagcctg
    16381 ctggcctttg attttgctgg cgttcatcac agcgatatgg tgacgctact ggcggagtac
    16441 ggtattgcct tgcgggccgg gcaacattgc gctcagccgc tactggcaga attaggcgta
    16501 accggcacac tgcgcgcctc atttgcgcca tataatacaa agagtgatgt ggatgcgctg
    16561 gtgaatgccg ttgaccgcgc gctggaatta ttggtggatt aatgacaaac ccgcaattcg
    16621 ccggacatcc gttcggcaca accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt
    16681 tgtcgcaatg ggaagataaa tatcgccagt tgatcatgct ggggaaacag cttccggctt
    16741 tgccagacga gttaaaagcg caggctaaag agattgccgg atgcgaaaac cgcgtctggc
    16801 tgggatatac cgtggctgaa aacggcaaaa tgcacttctt tggcaacagc gaagggcgca
    16861 ttgtgcgcgg cctgttggcg gtgttgttga ctgccgttga ggggaaaacc gccgccgaat
    16921 tacaggcaca gtcgccgctg gcattgtttg atgagctggg attacgtgcg cagcttagcg
    16981 cctcacgcag ccaggggtta aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg
    17041 tttaagcaca gaaccgggtc ggataagacg ttcgcgtctc atccgacctg attgtttaac
    17101 cctgacgcgc cgctttcgcc atcatcttct tcagcgcatg agaaaccgca acaaacccaa
    17161 aggtggcggt caccatcgtt gccgcgccaa atccagatgc acaatccatc cgcttcggcc
    17221 cttctgccgt ggctttcatc gcacacaccg taccgtctga ctgcgggtac accagcgctt
    17281 cagtagaaaa cacgcaatcc acgccgagct tacctttact gtttttcact acgccaaaat
    17341 cgcttttcag gcgctcgcgc aacttcgccg ccagcggatc ctgaatcgtt ttcgccagat
    17401 cggtaacctg aatctgcgtc gggtcaatct gcccacccgc gccaccggtc gtgaccagcg
    17461 ggattttatt gcgccgacaa taagcaatca gcgccgcttt gggccgtaca ctatcaatgg
    17521 catcaatcac gtacgaataa ccggcactca tatattgcgc tacgttatcc ggcgtcacga
    17581 aatcatccac caccgttaca cggcactccg ggttaatctg gcgaatgcgc tccgccatga
    17641 cttccgcttt tgccagcccg acgttatcgc gcagagcgtg aatttgccga ttggtattgg
    17701 tgacgcacac atcatccata tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg
    17761 cttccgccgc ccaggaaccg acaccaccga taccaaccac acaaatatgc gcgtcagcaa
    17821 acagttgcaa cgctttttca ccatacagac gcgctgtgcc accaaaacgc tgacgccatg
    17881 catcactaat taccacagac atagcacctc agaattaaaa agggcgaagt tatccctcac
    17941 cctaaacgca taaatgcctg atgcgctacg cttatcaggc ctacataact cctgcaatat
    18001 tttggatttg catgatttgt aggccggata aggcgttcac gccgcatccg gtatgaacaa
    18061 cgcgcactta gtcaataatc aggatgtgag gttacccctc ccctgtcata tccgtaaaaa
    18121 cggcatacag aatatcacat cagccgctaa agacgttacc tgcgcccgga gccgttttca
    18181 gcacccaaac ccgtccatag tggttgtacc aacctgcgcg gtgtccggct tccggcccga
    18241 tcccttgata gatatcgaag tgttggcctt tgattgcgcc cccgacatcc aacgccacca
    18301 tcagacgcaa ttcgtactga ccgttaaatt tgccgttatt atccagcagc ggcacttctg
    18361 ccagcaaggt agtacctggc ggaataattg aacgatcgga ggcaacagaa gcacggccaa
    18421 ccagcggcac cgcacttgcc cctttcaccg gtgcaaaaga ttgcggttta aagaagacga
    18481 aagacgggtt ctgttccagc agctcgcgaa cctcggcttc actgtgtgtt tcgccccaat
    18541 gacgaatcgc ctgcatcgac atatcttctt ttttcacttc gccacggtcg atcagcacct
    18601 taccaatgct gcgataggca tgaccgtttt tccctgcata gctgaaaaag ttaagcggac
    18661 tgccatcacc aaagtcgata tacccactac cctgcacatc cataatgaag ttatccatca
    18721 gagagttgct gtaagcgagg atgtatttat cgcccaatgc cccggcgtag atctccgcac
    18781 gagacggcag acgaccacgt tttggcggca tacggtaaat aggatactgg aactcgccct
    18841 ggcgggtatg gcgcgcctga attaccggcg tgtaatagcc tgtaaactgc acgttaccat
    18901 agttgtcggc accttccatc tgccaggcat caatgccgaa ctggcgcata ttgcgggtat
    18961 caccgcctgc gcgcagccac tcttgcaccg cgttataaac attactctgg ttgccataca
    19021 gacgcggtga cgaattacgg atatggttaa tttgctcggc aaaatcaccg gcgttaatcg
    19081 gcgcgccaac ggcatctggc tggttcacca gagagaaagg ctgggtaaat ttcccgtctt
    19141 tatattgctg tccgcgatcg gttggtttgg aagagcaggc ggcaagcatt gccacaaccg
    19201 tgcccataag aaggtacttt acccaacgtc ctttcattgt tctcttcttc aggttaaaaa
    19261 ataaggcgca acgaagataa caaaccgccg cgtttaatga aatgagcagc ctctccctga
    19321 cgcaaatttt gcacaaaaaa caggctttag tggtttgttt ttgttcaaaa tcatgctaaa
    19381 tccgtgatcg gggtaaaaaa aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc
    19441 acggacgcgg ggtggagcag cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt
    19501 tcaaatccgg cccccgcaac caatcaaatt tgatgaagta aaagcagtac ggtgacgcgg
    19561 ggtggagcag cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg
    19621 cccccgcaac caattattga acaccctaac gggtgttttt ttgtttctga tctcccataa
    19681 aaaaagcgcc attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca
    19741 aaatacgctt taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt
    19801 ttcagtttac gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga
    19861 atatctggtg cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg
    19921 ttgatttttc cgagcttatt cagtaccgct ttaccaaact taaggctgtc agcaatagtc
    19981 agcgactgca ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg
    20041 ccaccaatca agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt
    20101 gcgcctttgg ttgagagcgc aaacaccgag gaaccgctcg gctgacgact ggtaaaggcg
    20161 tcggcatgga tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact
    20221 tgcaacggaa tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctcttcc
    20281 tcgatcagag agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg
    20341 tatttcccca ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc
    20401 ggacgatcgc gccctgcttt acccggttgt ggaccacttt gcgctggcgg cacctgcttt
    20461 tcgaggtcgc ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt
    20521 gcattggccg gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg
    20581 gcaaacagct gcggttttac gttttgcttt aattcaaaaa ccatgcgtac ggtttgcggg
    20641 tcaaattgcc cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc
    20701 atccccttga gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg
    20761 ttactcaacg cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg
    20821 tagctggacg caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc
    20881 tgacttacgc tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt
    20941 gcagtgttgg atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt
    21001 aaacgactaa tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg
    21061 gtaaacattc tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt
    21121 tacacttgca ccctgcgaaa aaacagaata aaaatacaat aatttcaaat aatcatgcaa
    21181 aggggtgtgc cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg
    21241 ttccctatat caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca
    21301 ttgagcatga gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca
    21361 tccgtctggt ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc
    21421 accacgaacc gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg
    21481 tgaagcaggc tgcgggaaca ttgcaactgg atattactgc tcgcctgtca atgagtctca
    21541 ataacacgcc gctgcagggc gcgcatatca acgtcgttag tggcaatttt attattgccc
    21601 agccgctggg cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg
    21661 atgaagacgc gctccatcgt caactggaaa gcggtgcaat agtgctaatg gggccggtcg
    21721 ctgtttcagt cactggcgag agctttaatc tgacctcgga agagattgcc actcaactag
    21781 ccatcaaact gaaagctgaa aagatgattg gtttttgctc ttcccagggc gttactaatg
    21841 acgacggtga tattgtctcc gaacttttcc ctaacgaagc gcaagcacgg gtagaagccc
    21901 aggaagagaa aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag
    21961 cctgccgcag cggcgtgcgc cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt
    22021 tgcaagagtt gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc
    22081 agattcgtcg cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg
    22141 agcagcaagg tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat
    22201 tcaccattat tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg
    22261 aagagaagat tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac
    22321 ggggcgaggt tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat
    22381 tgtttgtgct gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg
    22441 atattgattt actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag
    22501 tgttgatggc ggatttaggg taagcgatga aaatcaccgg atgcggcagg cgtaacactc
    22561 gtgcgtcgca tccggcaatt acgtttattc ccgcgaacta aacaacgccg ccagaccact
    22621 gcgccgctca gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga
    22681 cagacgacga cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctaccggcgt
    22741 gcgttggctc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac
    22801 cgtcatcgcc cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt
    22861 gccgtccggc atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat
    22921 atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcggccttc
    22981 gtaccaacga gagtgcggat gacgatgaat tttgcgcttc tgttgcataa actgttcaat
    23041 tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta
    23101 ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata
    23161 acgtccgtaa cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa
    23221 aagtcgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc
    23281 accacggttg atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct
    23341 tttttgcagc aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg
    23401 aacgtgagtc cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa agcccgcgtt
    23461 ggcataagcg cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg
    23521 atcgccgaga aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc tcgacatcat
    23581 aggcagatcg atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc
    23641 ggcatgatga cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct
    23701 ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt
    23761 gccgagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg
    23821 gcagcgttcg ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt
    23881 tttaccggtg cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc
    23941 tgcaactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc
    24001 cagtagagct tcatcaacct caatggtatg attcacctca ttgaagaagc gcgctaccgt
    24061 ccgctcgtta caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt
    24121 gggttcatct cccctgctga ccgcttgaga agccagcaag cattcttccc aattttgtag
    24181 ctcaccgatt tcactgacac aggtcgccaa cagcggattc gacgcctcgt tattttccag
    24241 tcgtgaaagc ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc
    24301 gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg
    24361 gcgtagctgt ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc
    24421 tccagggtca taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg
    24481 gttgtgtaaa tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata
    24541 acgccgccaa agtgaagatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc
    24601 agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc
    24661 atagcctgtt gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc
    24721 aggtaataac gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt
    24781 acctgcatga actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt
    24841 gcatcaagct ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc
    24901 tgtttatcgc ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg
    24961 aggacagccg tgatccactc ggtcaaaacc ggttcccact gcgattcaaa gccgccgagt
    25021 tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac
    25081 aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc
    25141 ggttcttcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc
    25201 tgagcgatac cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg
    25261 ccgggcaatc gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc
    25321 cagggctggt tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct
    25381 tcaatacagg tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg
    25441 agcgcacttt ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt
    25501 gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga
    25561 tcttccgcca gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc
    25621 aaaactgcct caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg
    25681 gtaataaatg gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca
    25741 atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct
    25801 ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt
    25861 ccggcttctt gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct
    25921 gccgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc
    25981 ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc
    26041 cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac
    26101 gttgccagcg cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacaac
    26161 caaagcattt cctgcgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg
    26221 gaagggattt ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc
    26281 accagcacgc tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac
    26341 gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc
    26401 atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc
    26461 gcaggctgcg tttcaccttt aaatacaaaa cgtaacgcct gatttttccc ggctgatttc
    26521 actggaataa acggtatttc gcgaaacatg aacgtgtcat cagtctggct gaaaagcttg
    26581 ttcacactgt tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggca
    26641 tgaacttcac tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg
    26701 gcctgcttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg ccagatacgg
    26761 cgaaaaattc ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg
    26821 aatcgcgtac ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg
    26881 agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact
    26941 gtttcgcgga tctcagccaa tgcgcgggtg atcaacagat cgcggatcga caatggttct
    27001 gcaagcagtt gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggccttc
    27061 gtgcgatctt ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg
    27121 tttgtctctt cttctgccca ggcgctgatc ttttcgatcc atttagcctg attgctacgg
    27181 ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg
    27241 tcgcgccact gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg gcgggaagcc
    27301 agcgtttcat catcgggcgg cggtgctttg ataaccggcg gttcgccttg cagataacga
    27361 ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt
    27421 tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc
    27481 agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg
    27541 ttgagcatgc gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt
    27601 tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggcgt
    27661 tcgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg
    27721 ctacggatac gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt
    27781 tcttcaacga tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc
    27841 aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc
    27901 tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc
    27961 actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc
    28021 aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat
    28081 gccttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag
    28141 ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc
    28201 tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat
    28261 taccgcctgc tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc
    28321 ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg
    28381 caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata
    28441 gcccaattgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa
    28501 cagagagcta taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc
    28561 cagtgcggag tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac
    28621 cactacatct ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc
    28681 gcgtgccagc gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg
    28741 agcccctgat tttaaggcgt cgcgataggc cagcacctct ttcaacgtaa tggagggcaa
    28801 aattttacgc cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat
    28861 aatcgcctgc tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga
    28921 cttcgcctgc tcaagctgct cttccgtagc ggtatagcta aagtacccct cgagcaatgc
    28981 ctggaacagc tgcggcagac gctgggtgta accattagca ttaatcataa ggccgttgtt
    29041 agcgttggtg gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc
    29101 cagccctgcg agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc
    29161 tttcggatta cgcaaaatca ggctgacatc agctttgggc tcgctggcaa aataacggct
    29221 tggcgcatac accacgcgca gattcgactc atcaacaatc agctctggat ggtcgtattt
    29281 cttctctgac ttaatcagcg agaaatcatc aggaatataa gggttaagct ctggcaaaga
    29341 gagcgcaatg tcggcggctt ttttctgcca gtcggcgaaa gtttgtgcgc tgattttatc
    29401 gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt tcgggctgat
    29461 ataccagata cgcgcattct gcggcgtcat catcgccaga cgttccttta ctgctttagc
    29521 atcgtaccga tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa cgcgaatcat
    29581 ggtatctgcc agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc
    29641 gatatccagc acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa
    29701 cagattgaga tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc
    29761 agttaaagac gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt
    29821 ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctgggct
    29881 gcgattgcca atcagatagg taatcaattc atcggtttta ctacggaact tcgctgagtt
    29941 gttatcgatg cgaaactcaa cgcgcaacac tttacgcggc agcgcaggga cgtaatgaat
    30001 gataatgccc ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc
    30061 tttgttcggc acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt
    30121 attactgtaa ataaccgcct tcatcaaatt ggcggaatag tacttctcgt ggaaatcttt
    30181 cagcgcctgc tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt taccaccaga
    30241 aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat
    30301 cccgtcacgc gtacgcgcca tggttaattc agcgttcact gcattacgct cacgttcggc
    30361 gtatttcttg tcgagcaagg gttctgcaat agcatcggcc aggcggtcta ccgcaccggg
    30421 caaggcgtcg ttctcaactt ccagatagaa agccgtgcga tacggcgcag tgctggcatt
    30481 gtgactaccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt
    30541 cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc
    30601 ttccagcgac ccaacgggca ctaccagcgc cgagagcgat ttaactgcct gcggatcaga
    30661 aaccagcaag accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt
    30721 atcactttta cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaatgg
    30781 tgcccagagg gcaacaaaca acaataatgc tttgaaccag gtgctgcggg gcattcacgg
    30841 acctcataat caactttatt ttctggacag attcaacagg ttaacgcaaa tcatctgcca
    30901 gaatttaatc ttgtgctgca cgagtcagcc tatgtttata taaccatcag tccgtgactg
    30961 gtgcgcatca taaagtaagc agatagattg cgcaattttt atacaacact catgactgat
    31021 taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct
    31081 ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg ccacgcacca
    31141 tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt
    31201 catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt
    31261 caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc agttgtgaga
    31321 ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcaccg tctttgcgta
    31381 gaaaaaggcg actttcgcca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa
    31441 gttgcattcc ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct
    31501 gcacctgcgg caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac
    31561 tctgccccgg ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact
    31621 gtgtttccca gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc
    31681 ggcggaacaa gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga
    31741 tttgataacg gctaagtcct tccagaataa atggctcggt gtctgggatt tcgctgtctt
    31801 cagtatggaa gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga
    31861 atcgttgtag cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa
    31921 attcagaatg tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt
    31981 gtcgttcgcc tggctggtag ttttgcggat caaacggcat ccgggtatgg aggcaagtaa
    32041 gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat
    32101 aatgactttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac
    32161 tgttatcctg aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta
    32221 acgcttccag gaacaggtag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt
    32281 tctggctcat caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca
    32341 ttcccagcag gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt
    32401 taaccggtcc ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt
    32461 catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga
    32521 taatcgcctg ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca
    32581 ggaagaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct
    32641 gtgccagccc gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca
    32701 gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg
    32761 cgctctccat cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc
    32821 cggtggcggg gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg
    32881 attcgttgac ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg
    32941 ccagcaccgg cacatccagc agcgccagca catcctctga cacaaagcga ctgtcaggca
    33001 gtgataacag gctgataaac gcttccagta ccggatgtga ctgccgcgca cgacggtcgg
    33061 aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc tgaataaacg
    33121 gactgtagct gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgggg
    33181 cttcctccag catcgccagc aagcgatcgt gtaaaacttc aacttcacgc tgcgggctat
    33241 ggcaaacgtg gaatgtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac
    33301 gggaaaactc ttcgatgttc acacccgcaa cggcgcggtt ttccagttcc agaatgtcag
    33361 actgaatgtt atgcagcagg ttatctggcg tcacatcgac aaaagcgtcc agctcctggc
    33421 tgctctccag gtcagaaagg agataaatgt agtcgcgccc aagcttcccc catgaggcca
    33481 gcagcgagtt gccgacatcc tgttcaccat cgctgttaaa gagctgtccg gcattttcgc
    33541 tatcgcgaaa tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgagtcagca
    33601 gtttcgccag ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa
    33661 acaggagatg gatttcaata tgtttaccca gcgcctggag cgcctggaga taaacaggcg
    33721 gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg
    33781 cggactccag cgtttcgata aagcgctgat agagattcgc gcggtgccag cgcggttgtc
    33841 cgagttcatg ggtatattcc accagcgcct tccacaacgg ggcttgccag gcctgtgctt
    33901 ctcccagccc ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga
    33961 ccagatactg gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt
    34021 cgctatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg
    34081 gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga
    34141 tttccggtaa cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa
    34201 tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg
    34261 tactttgcac cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa
    34321 taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag
    34381 cggctcctga ctactgacaa ttcgggcaat gcaggcgggt catctcgcct tctctgcccc
    34441 cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt
    34501 tgacctgcca gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc
    34561 gccagagctt ttggtactgg tttctgctgg cgagactgtt cattgatgtt cgctggatac
    34621 ccgataacgc agtgacaatc atcaccatca gcaccatcag caccatcagc accatcagca
    34681 ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg gaagctgaca
    34741 taatgccccc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa aaacaatttt
    34801 tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg caatcaataa
    34861 ggcttcatta tctgccagta aacgcaaaca aacctgggca tcggttccag cgtactgcga
    34921 gcactgaact gctggctgcg tctgccagga gtgcattttt ccccacgcca gcgccgactg
    34981 aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg caaaactgcg
    35041 atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca tcaggaccag
    35101 agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac gctatagctg
    35161 gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac cgtcaacacc
    35221 ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat gatggcatcc
    35281 ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc
    35341 agcacatgct ccttcagacg aaatccaatc tggtcggact cttttaccgg ttcgcgatcc
    35401 cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg tccgacaatt
    35461 tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag atgcttcgcg
    35521 acggtaaata cccgcagcca gatttcatct tccagtgcca gcttacgagt gctcgttaaa
    35581 ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac gctactgatc
    35641 gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg catccttctg
    35701 tttctccttg ctgataaagc cggagtcttc cccacggcga aaccaccagc caccactcgc
    35761 ccgttgagtt tttgaagcga atatgcacgg cccatgcggt attgcgcagg ccaaagaaag
    35821 caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca aagaccaatg
    35881 gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac cataacgtcc
    35941 cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat
    36001 agagcaaata gtcccgcgcc tagctggcgg tttgccaaag ccgttgcgac tgctgccagt
    36061 attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc gcgaccagcg
    36121 tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc gaggagaaaa
    36181 aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta cgccgttgca
    36241 ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta aaaaaaccga
    36301 cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc ggcgctttaa
    36361 cgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag tcgaagatgg
    36421 attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg cttaattgca
    36481 gatgagtttg atccatatgg ttgctgtaca ggtgcgtatc gccaccggtc cagacaaaat
    36541 cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg tagctggcaa
    36601 tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg caagagagtt
    36661 tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc attttatcca
    36721 gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg tttttcagct
    36781 ggttcagtac cgtagtaatc tggtcaatat gacgaccatc tggcgttggc caggcgcgcc
    36841 actgtttacc atacactggc ccgaggtcgc cattttcatc ggcccattcg tcccagatgg
    36901 tgacattgtt ttcgtgtaga taagcaatgt tagtatcgcc ctgcaggaac cacagcagtt
    36961 cgtggatgat ggaacgcagg tggcaacgtt tggttgtcac cagcgggaat ccatcctgca
    37021 ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta cggtcgtttt
    37081 tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc atggttcctc
    37141 aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc acacccgcga
    37201 caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag gcaccggtaa
    37261 actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg tagccaatca
    37321 ggaacaagcc tgagacagct cccattgggc gcggtttacg aatatacagg ttgaggataa
    37381 taaacagcac cacaccttcc agcagcagtt cgtaaagctg tgatgggtgg cgcggcagca
    37441 caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc aaaatatctt
    37501 ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg cggccccaca
    37561 attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga atgagtggtg
    37621 cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag ataatcatca
    37681 ccacgataac gccaatcagg ccgccgtgga aagacatgcc gccgtcccag acacggaaca
    37741 gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa ccaatacgtc
    37801 ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca tttttggtcc
    37861 agccactgcc cggacgattc gcccgacgtg ttgccagcca cattgcaaaa atgaacccca
    37921 ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa atgaccggat
    37981 caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc ttgttatttc
    38041 gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc aaaggtgcga
    38101 ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat gtatatgatc
    38161 cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata aaggctgcaa
    38221 cctgatgacg aacttcggtc gccagttgcg cttccagact acgctgcgca agattttctg
    38281 cttcggcaaa atcaatgcgc cgcagcaggt attttacccg cgctacagaa cgtccgttca
    38341 tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga tcgcccgcca
    38401 tttcaccgca caaacgaaga tcgataccat gtatttccgc ttcccgggcg atcatcgcca
    38461 gagctcgtaa cattgcagga tgaagactgt cgtaaatgtt cgccacccgg gtattgttgc
    38521 gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg aaatcgaccc
    38581 tctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg atgccgatac
    38641 gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca cgttcaatca
    38701 ggcggcgtgc ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga atattcaggt
    38761 tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag atctccggct
    38821 gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg ctgatcggca
    38881 tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg ggtttatcat
    38941 tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac gggaaaccac
    39001 tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata cagctgccca
    39061 gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata cgctcaccgc
    39121 tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc tcaatctctt
    39181 cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc agcaattcac
    39241 cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga atatccgcgc
    39301 ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag ttggctgcgc
    39361 catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga gttgtcgctg
    39421 acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct tgattagcgt
    39481 catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcaccagcc cgctctttga
    39541 gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc gtttttaccg
    39601 cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc agccgggtgt
    39661 ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt gctccggcgg
    39721 caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc ccggtcagtc
    39781 gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc attaaaggta
    39841 acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt aatgcgcgga
    39901 ttcgcgtctg gcgatattgc ccaaacaggg cagtcaactg cgactgagaa agaatagctg
    39961 ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac tggcgcaact
    40021 ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt ggtacgccta
    40081 aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg gggtgctttt
    40141 gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg atcccttcat
    40201 caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc gccatcaggt
    40261 agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta tccatcgcaa
    40321 gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg ctggctacct
    40381 tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct cttttacgtc
    40441 gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc accacactcg
    40501 cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc tgtctgaccg
    40561 gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg gtttgcatat
    40621 tgatttctgc atcgccgctt accagctgca agagaaacca tttttgtttt tggccgatac
    40681 aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc aaccagttac
    40741 gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca aacaattcac
    40801 ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga aattgccagg
    40861 agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa atcacgatac
    40921 ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat aaagctttat
    40981 atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca gggcattaca
    41041 cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga ggaaggggtg
    41101 gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac ggaattacta
    41161 cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat attcattatt
    41221 agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac ccaggtcaca
    41281 cctctcattt acgggttggc gaaagatcaa ccaatgccgt gtttatccac agaatgtgcc
    41341 actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa ggctgtaaat
    41401 gaaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa catggtgtaa
    41461 gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg cgcaactcga
    41521 ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacatg tggaaaaagt
    41581 tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt atcatgacat
    41641 gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt tagcgcaagc
    41701 acagcaactt tctggttata cattgggaga actggcggca cttgacgggc tggttacgcc
    41761 agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct ggctaggtgc
    41821 cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac ttaaaactat
    41881 ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg cccggttaac
    41941 gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca aacgcgtact
    42001 gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg taggatcacc
    42061 gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact gggaagaatt
    42121 aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg gggaatattt
    42181 acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg gtgcccgggg
    42241 cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca ccagtgcact
    42301 actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt tccgggcata
    42361 taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg gataaccgat
    42421 cctaacgctt ggcttgcgct cggtacgctg acgctgctgg agatcgttct tgggatcgac
    42481 aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg tgctcatgcg
    42541 cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc atcaatctct
    42601 tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat ttctgcccgt
    42661 gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa ggaaatccac
    42721 gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt cctcggcgct
    42781 atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat taccgctgtg
    42841 ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt aggcgtgatg
    42901 atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt aaaaatgctg
    42961 gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt cgacatccac
    43021 gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga aagcctcaac
    43081 ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc gggagggcgt
    43141 actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat ataacgtcgg
    43201 tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg aaaaaatggg
    43261 cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc agtgattacg
    43321 tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa attgatgatg
    43381 ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt aaccgtgatg
    43441 atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg ccttaagatt
    43501 tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc gtgattataa
    43561 aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag tcagcacgct
    43621 ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc acgcacaact
    43681 cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt acccagtacc
    43741 tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc tggcgaaaca
    43801 tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc gcaataatga
    43861 caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag cgctgcatga
    43921 cagcctcaag cgcctacaga ctgattacct cgatctttat caggtgcact ggccgcagcg
    43981 cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc ctgcggtttc
    44041 gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa ttcgttatat
    44101 cggcgtgtcg aacgaaactg catttggcgt aatgcgctac ctgcatctgg cggacaaaca
    44161 cgatctgccg cgtattgtca ccattcagaa cccttacagt ctgttaaacc gcagttttga
    44221 agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct attcgtgcct
    44281 gggtttcggc acgctgaccg ggaaatatct caatggtgca aaacccgctg gcgcacgtaa
    44341 tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag ccgtcgcggc
    44401 gtatgttgat atcgccagac gtcatggcct tgatcctgct cagatggcgc tcgcgtttgt
    44461 acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg atcagctgaa
    44521 aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg aaattgaagc
    44581 ggtacatcag gtttatactt atcctgcacc ataaagatta acgccggtta aaaccggcgt
    44641 taaatattaa tgacggcgct gccagatcca cagcgccgtt attgccagcg caaatagcgc
    44701 accgaagcca atgccaatgg gcacgaccgg gatgcctacc attaccgcca gcgagtaaat
    44761 gcccagcatc aacaacatgg cgctgttttc gccaaggttt tgtactgcaa tcgcattccc
    44821 cgccccgacg ctttttttac cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa
    44881 gccccccagc acgccaatca gcatcaacaa ggcataggct ggcagcagct cgtgttgcag
    44941 ggagaaaatc ggtaccacca cgccaatcaa aatcccggct ggcatacagc gtgacacggt
    45001 ttccagcgta accagcttcg ctgccgcacc tgcgccaacc acgataccaa tcgctaccat
    45061 cgcgttgaga taggtcggcg tagcgttatc ggtaatgccc agcgccaccg gtacccacag
    45121 caccaacagg aaacgcagcg tgacacccgc tccccagaat aaactggtgc ccaccagcga
    45181 aaaacgcgtt tcaccattgc accatagcga ggtgcaggca ttcaggaaac tgcgggtcat
    45241 gttgatgaga ttccaggact gccccggacg cgccgccagt ttgggaatgt agatattggc
    45301 aacgaccgca ccaccgtagg ccagtgcgca tgcggccaga gcgaccagga catgccagtc
    45361 agccagcaca ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat
    45421 taaaccgtta gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt
    45481 cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat
    45541 accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg
    45601 gcctttggcg aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc
    45661 tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa
    45721 cgccagagtg gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat
    45781 aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt
    45841 gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa
    45901 gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt
    45961 gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca
    46021 ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg
    46081 aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg
    46141 caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat
    46201 cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt
    46261 ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt
    46321 gcaggcgtcc gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta
    46381 gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg
    46441 cgcattcggt cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct
    46501 gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt
    46561 catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg
    46621 tgcaactgcg gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa
    46681 ggaacacttc tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt
    46741 gaaacagcgg taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa
    46801 tctgctcgac gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt
    46861 cagaaccgga ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca
    46921 ggcgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt
    46981 ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt
    47041 tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag
    47101 cactggtcag cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg
    47161 cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc
    47221 gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca
    47281 atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga
    47341 agcggtacat tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac
    47401 gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac
    47461 gcggcgcatc cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga
    47521 acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa
    47581 tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga
    47641 ttttcatcag cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc
    47701 cctgttccac cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa
    47761 caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa
    47821 cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga
    47881 cgtgattagg cgtaattagt acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc
    47941 gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct
    48001 ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga
    48061 cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg
    48121 tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct
    48181 gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg
    48241 tgtaaattgt cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc
    48301 gcaggctggt gtaattcatg tgctcaacac gaagttgact tcacctatca atacctctgg
    48361 gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc
    48421 gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc
    48481 acattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga
    48541 aacaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg
    48601 actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ccaaagccag
    48661 cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc
    48721 caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt
    48781 ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg
    48841 taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga
    48901 gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccagatgc
    48961 cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc
    49021 tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg
    49081 tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc
    49141 tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattccggc
    49201 caatgatcgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac
    49261 cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc
    49321 ggcgggcgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt
    49381 aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg
    49441 ttacccaatc gtaacgatgg cgacccaggc tgccgaactg gctttggcac tggcggataa
    49501 tcgccctctc ccggaaatta ctaatgtatt tagtccgacg ctggtacgtc gtcattcagt
    49561 gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca
    49621 attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat
    49681 tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac attgatgcgc
    49741 catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc ggcaaggcgc
    49801 gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca cataacggcc
    49861 cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg
    49921 cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat
    49981 caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaata
    50041 cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca gggtggccca
    50101 aatggcgagc gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa
    50161 ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta
    50221 aatcctgacc gaattcgata acctgacgca ccatagcacc acacacctgc tccagatggg
    50281 cataatcaac gccagaacca atgtgcatgt gaatgccgac tagctgcaga tgatgacgtt
    50341 gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc
    50401 caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg cgcagccata
    50461 cccgatgccc tggcgaaacc tggcccagtt ggtcgagcgt atcaacagaa cccgcattca
    50521 ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa
    50581 aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt
    50641 cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa
    50701 tattggaaca ggctttctgt gcaaagcgca ccacttcaaa ctgtttcagc gctgcaatct
    50761 gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac
    50821 gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa
    50881 actccagatg agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga
    50941 tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg
    51001 ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga
    51061 ctgaggcagc acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct
    51121 ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tggacgatta catcctaccg
    51181 tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg
    51241 tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc
    51301 cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg
    51361 atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc
    51421 agcgtcatga tttaggactc actgagacac tccatacgcc tacgggaaca gaacgtaccg
    51481 aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa
    51541 aggtattaac gccggatgat tttcagggtg agaattacat cagcctttcc cgtactgaca
    51601 gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg
    51661 tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtatttcgg
    51721 tggttaaccc gctcaccgca ctagattatg cggcaagcgg tttagtggtg cggcggttca
    51781 gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc
    51841 tggttcaggc gtttagtggg catttacaag cggggttacc gaaactggtc acttctcttg
    51901 acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc
    51961 cgcggtatca aacacaggca aaacactgcg ctcttctggc accagtaaac caatttctgt
    52021 gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata
    52081 ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt
    52141 aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccgtcag
    52201 ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt
    52261 cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt
    52321 gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc
    52381 ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc
    52441 ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcat
    52501 ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta
    52561 aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc
    52621 ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag
    52681 gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat
    52741 cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc
    52801 gtgacatttt tggtttccgg aatgagccag aaagtaatac ccacaaacgc aatgttcagc
    52861 gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggcc
    52921 gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg
    52981 cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc
    53041 gcataaccgg caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa
    53101 gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc
    53161 acgctgaaac caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca
    53221 atgaaggtcg cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct
    53281 gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt agtacatgat gatgttcata
    53341 ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta
    53401 cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc acgaatttcg
    53461 ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc
    53521 tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga
    53581 ataatcagca gaactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta
    53641 taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg
    53701 tacatactga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga
    53761 gcggtgtaag acgcgatccc gacagcaatg cccagcacca cacgagcggc gattaacatc
    53821 tctacgctgg tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc
    53881 atcaggctgt atttacgccc caggcggaac gacagccaac cattaaacag cgcaccaatt
    53941 gctgcgccga gcatcatgct actaaccacc cattcctgca aacgactggt cagcacaaag
    54001 tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat
    54061 cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgcgtatc acgcaaagaa
    54121 cgtggcgtta aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta
    54181 agacattgtg aaaaaatacg tgaacaattc acacaggtgt caggtcggaa acagcataaa
    54241 tatggattaa attgctgcga catgccgtta tgtgatggat attccaattt taaaattaag
    54301 ttgaattatt gagattatta ttaacctcct aattttaaag cagataaaat tcataaagtt
    54361 cattaattga taattgatat ggattatttc ataaccatga tatggattat gatgatctag
    54421 agatataaaa aaccctgcca tgcggcaggg tcataaaagt aagaggaatg aattaacgcg
    54481 ccagccaacc gccatccaca gcaatggtat aaccatttac ataatctgaa gcgctggagg
    54541 caagaaacac taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc
    54601 gatcgagaat ttccgcgcta cgttgttcat ctgcccgcag ttgttgggta ttgttggtcg
    54661 ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat tcgtttgcca
    54721 tcaatcgcgt cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc
    54781 cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga
    54841 taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga
    54901 catcgtccca gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt
    54961 tattcaccag gatatcaata tgaccaaatt cagctaccgc gcgatccagc agtccaggaa
    55021 tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga
    55081 cctgctcgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt
    55141 gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa
    55201 ctttaccttg gagagaaaat gcacttaaaa tcataacaac accttattcg tttatgccca
    55261 caactagcgc aaatctttaa cgcccacatg gtccatatca ttaaagacct ggttttcacc
    55321 gaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct
    55381 cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggctgccc
    55441 catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg
    55501 gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag
    55561 ccccatactc aattgacagg tttccagtac atccgggaca aaatatttgt taatcgtgcg
    55621 acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt
    55681 tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc
    55741 ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcgtcgcg
    55801 gtgaccgatt tcatagcatt gaccatcgac agtaatcgta ccggcaccgc caatattgat
    55861 aacacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc
    55921 gccaacggaa accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg
    55981 gctgtaaacc atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg
    56041 cagcccttgg gtatccagcg tttttgcgtg cgcaccgtgg atgctctgtc ttacgtccac
    56101 atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg
    56161 ttttctctat aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa
    56221 tgttttattt ttaatttaag gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga
    56281 gaatggaaaa atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa
    56341 cagattaatg acggcagata tttatcggta aggaggaaat gcagagctac tcgtcacgtt
    56401 caatggtcaa tgccacgccc tggcccccgc cgatacaaag cgttgccagc cctttgcggg
    56461 cattacgttt caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta
    56521 tcgggtgacc gagtgcgatc gcgccaccat tgacattgac ccgacgctca tcccactcaa
    56581 gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga
    56641 catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa
    56701 ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg
    56761 gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt
    56821 ttatggatga tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac
    56881 gggctaaacc ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg
    56941 tctgaccgtt actttggatc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa
    57001 tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga
    57061 tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat
    57121 cccacaaccc atcatgcacc agactgtcaa ccaactggct attgccaagc tgttcaccgg
    57181 tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca cctgcgatga
    57241 caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagaccgg
    57301 aaccgcaaac gtcattaatc gtgattgcag aaacgctgtt aggaagacca cctttaatag
    57361 ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt
    57421 catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg accagactac
    57481 caagttccac ggcggaatga cccgctaacg caccacgaaa gcagccgata ggtgtccgta
    57541 acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt
    57601 aaatgattgt taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg
    57661 aaatatgcct gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc
    57721 caccaataga tgggtagata gggcgttaat ctcccatact taacttggtt tatggtaaat
    57781 tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct
    57841 aaagaagaaa ctctgtgaag tttcgcgctc tatggcacag ccgttgatgc aggcacgcta
    57901 ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc
    57961 gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct ctcatcaaaa
    58021 acatcagcag gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt
    58081 aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg
    58141 gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc
    58201 cgtatgttgg tgagtctggg tgttgtatta attctgaatc tcatttttct gatgggacgt
    58261 catgccacta ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt
    58321 ctttccattt accttgtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc
    58381 aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc
    58441 catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct
    58501 atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg
    58561 ttctttgtct ttagctgcct gctttctatt ccatcttcgt atattgaagc ggctaaagaa
    58621 gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca
    58681 atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt
    58741 ggcgttattg aaggtgccac agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag
    58801 aaaagtcgtg catttaacca cgcactatca attatgttgg tatcgctgat taccttcatt
    58861 gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt
    58921 gccatgatac ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc
    58981 tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg
    59041 ttctttagct aatatccgtt gtggatggtt caaaattgct acaattttga accattccat
    59101 ttttcataat attcatttgt tattattccg ccctatcact tctctcgtca tatcctagaa
    59161 ttatcagtta gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg
    59221 cataacttag attcgcaaga tatcttctgg tcattcaaaa acaatttgcg atatagatat
    59281 tctcatacca tgagatagtt atc
//
LOCUS       DAZPFP010000007        55529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPFP010000007 DAZPFP010000000
VERSION     DAZPFP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.007"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            106..1592
                     /locus_tag="ACHUR4_000367"
                     /pseudo
     CDS             106..1592
                     /locus_tag="ACHUR4_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1607..2341
                     /gene="ypdB"
                     /locus_tag="ACHUR4_000368"
     CDS             1607..2341
                     /gene="ypdB"
                     /locus_tag="ACHUR4_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1454117.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2354..3211
                     /locus_tag="ACHUR4_000369"
                     /pseudo
     CDS             2354..3211
                     /locus_tag="ACHUR4_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3214..5716)
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000370"
                     /pseudo
     CDS             complement(3214..5716)
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5741..6785)
                     /gene="ypdE"
                     /locus_tag="ACHUR4_000371"
                     /pseudo
     CDS             complement(5741..6785)
                     /gene="ypdE"
                     /locus_tag="ACHUR4_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6785..7201)
                     /locus_tag="ACHUR4_000372"
     CDS             complement(6785..7201)
                     /locus_tag="ACHUR4_000372"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR1454118.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7198..7305)
                     /locus_tag="ACHUR4_000373"
     CDS             complement(7198..7305)
                     /locus_tag="ACHUR4_000373"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454119.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7266..7868)
                     /gene="ypdF"
                     /locus_tag="ACHUR4_000374"
                     /pseudo
     CDS             complement(7266..7868)
                     /gene="ypdF"
                     /locus_tag="ACHUR4_000374"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7883..9130)
                     /locus_tag="ACHUR4_000375"
     CDS             complement(7883..9130)
                     /locus_tag="ACHUR4_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1454120.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9152..9478)
                     /locus_tag="ACHUR4_000376"
     CDS             complement(9152..9478)
                     /locus_tag="ACHUR4_000376"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1454121.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9697..10662)
                     /gene="glk"
                     /locus_tag="ACHUR4_000377"
     CDS             complement(9697..10662)
                     /gene="glk"
                     /locus_tag="ACHUR4_000377"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1454122.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10866..12122
                     /locus_tag="ACHUR4_000378"
     CDS             10866..12122
                     /locus_tag="ACHUR4_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1454123.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12237..12563
                     /gene="ypeC"
                     /locus_tag="ACHUR4_000379"
     CDS             12237..12563
                     /gene="ypeC"
                     /locus_tag="ACHUR4_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1454124.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12704..13942)
                     /locus_tag="ACHUR4_000380"
     CDS             complement(12704..13942)
                     /locus_tag="ACHUR4_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1454125.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14278..15480
                     /gene="nupC"
                     /locus_tag="ACHUR4_000381"
     CDS             14278..15480
                     /gene="nupC"
                     /locus_tag="ACHUR4_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1454126.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15530..17642)
                     /locus_tag="ACHUR4_000382"
                     /pseudo
     CDS             complement(15530..17642)
                     /locus_tag="ACHUR4_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17850..17925)
                     /locus_tag="ACHUR4_000383"
     tRNA            complement(17850..17925)
                     /locus_tag="ACHUR4_000383"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17890..17892),aa:Ala,seq:ggc)
     gene            complement(17965..18040)
                     /locus_tag="ACHUR4_000384"
     tRNA            complement(17965..18040)
                     /locus_tag="ACHUR4_000384"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18005..18007),aa:Ala,seq:ggc)
     gene            18261..18620
                     /locus_tag="ACHUR4_000385"
     CDS             18261..18620
                     /locus_tag="ACHUR4_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1454127.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18622..19014
                     /locus_tag="ACHUR4_000386"
     CDS             18622..19014
                     /locus_tag="ACHUR4_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1454128.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19066..20481)
                     /gene="gltX"
                     /locus_tag="ACHUR4_000387"
     CDS             complement(19066..20481)
                     /gene="gltX"
                     /locus_tag="ACHUR4_000387"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1454129.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20740..20815
                     /locus_tag="ACHUR4_000388"
     tRNA            20740..20815
                     /locus_tag="ACHUR4_000388"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20773..20775,aa:Val,seq:tac)
     gene            20860..20935
                     /locus_tag="ACHUR4_000389"
     tRNA            20860..20935
                     /locus_tag="ACHUR4_000389"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20893..20895,aa:Val,seq:tac)
     gene            20940..21015
                     /locus_tag="ACHUR4_000390"
     tRNA            20940..21015
                     /locus_tag="ACHUR4_000390"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20973..20975,aa:Lys,seq:ttt)
     gene            21046..21171
                     /locus_tag="ACHUR4_000391"
     ncRNA           21046..21171
                     /ncRNA_class="other"
                     /locus_tag="ACHUR4_000391"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21291..21632
                     /locus_tag="ACHUR4_000392"
     CDS             21291..21632
                     /locus_tag="ACHUR4_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1454130.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21623..22549)
                     /locus_tag="ACHUR4_000393"
     CDS             complement(21623..22549)
                     /locus_tag="ACHUR4_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1454131.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22639..23637
                     /locus_tag="ACHUR4_000394"
     CDS             22639..23637
                     /locus_tag="ACHUR4_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1454132.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23634..23852)
                     /locus_tag="ACHUR4_000395"
     CDS             complement(23634..23852)
                     /locus_tag="ACHUR4_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1454133.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23854..25869)
                     /gene="ligA"
                     /locus_tag="ACHUR4_000396"
     CDS             complement(23854..25869)
                     /gene="ligA"
                     /locus_tag="ACHUR4_000396"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1454134.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25940..26926)
                     /gene="zipA"
                     /locus_tag="ACHUR4_000397"
     CDS             complement(25940..26926)
                     /gene="zipA"
                     /locus_tag="ACHUR4_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1454135.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27156..27917
                     /gene="cysZ"
                     /locus_tag="ACHUR4_000398"
     CDS             27156..27917
                     /gene="cysZ"
                     /locus_tag="ACHUR4_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1454136.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28102..29073
                     /gene="cysK"
                     /locus_tag="ACHUR4_000399"
     CDS             28102..29073
                     /gene="cysK"
                     /locus_tag="ACHUR4_000399"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1454137.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29457..29714
                     /gene="ptsH"
                     /locus_tag="ACHUR4_000400"
     CDS             29457..29714
                     /gene="ptsH"
                     /locus_tag="ACHUR4_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1454138.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29759..31486
                     /gene="ptsI"
                     /locus_tag="ACHUR4_000401"
     CDS             29759..31486
                     /gene="ptsI"
                     /locus_tag="ACHUR4_000401"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1454139.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31527..32036
                     /gene="crr"
                     /locus_tag="ACHUR4_000402"
     CDS             31527..32036
                     /gene="crr"
                     /locus_tag="ACHUR4_000402"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1454140.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32078..32929)
                     /gene="pdxK"
                     /locus_tag="ACHUR4_000403"
                     /gene_synonym="thiJ"
     CDS             complement(32078..32929)
                     /gene="pdxK"
                     /locus_tag="ACHUR4_000403"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1454141.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33034..33408
                     /locus_tag="ACHUR4_000404"
     CDS             33034..33408
                     /locus_tag="ACHUR4_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1454142.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33441..34164
                     /locus_tag="ACHUR4_000405"
                     /pseudo
     CDS             33441..34164
                     /locus_tag="ACHUR4_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34368..35279)
                     /gene="cysM"
                     /locus_tag="ACHUR4_000406"
     CDS             complement(34368..35279)
                     /gene="cysM"
                     /locus_tag="ACHUR4_000406"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1454143.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35413..36510)
                     /gene="cysA"
                     /locus_tag="ACHUR4_000407"
     CDS             complement(35413..36510)
                     /gene="cysA"
                     /locus_tag="ACHUR4_000407"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1454144.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36500..37375)
                     /gene="cysW"
                     /locus_tag="ACHUR4_000408"
     CDS             complement(36500..37375)
                     /gene="cysW"
                     /locus_tag="ACHUR4_000408"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1454145.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37375..38208)
                     /gene="cysT"
                     /locus_tag="ACHUR4_000409"
     CDS             complement(37375..38208)
                     /gene="cysT"
                     /locus_tag="ACHUR4_000409"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1454146.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38208..39224)
                     /gene="cysP"
                     /locus_tag="ACHUR4_000410"
     CDS             complement(38208..39224)
                     /gene="cysP"
                     /locus_tag="ACHUR4_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1454147.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39528..40319)
                     /gene="ucpA"
                     /locus_tag="ACHUR4_000411"
     CDS             complement(39528..40319)
                     /gene="ucpA"
                     /locus_tag="ACHUR4_000411"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1454148.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40448..41306)
                     /gene="murR"
                     /locus_tag="ACHUR4_000412"
                     /pseudo
     CDS             complement(40448..41306)
                     /gene="murR"
                     /locus_tag="ACHUR4_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41470..42366
                     /gene="murQ"
                     /locus_tag="ACHUR4_000413"
     CDS             41470..42366
                     /gene="murQ"
                     /locus_tag="ACHUR4_000413"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1454149.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42370..43794
                     /gene="murP"
                     /locus_tag="ACHUR4_000414"
     CDS             42370..43794
                     /gene="murP"
                     /locus_tag="ACHUR4_000414"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR1454150.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43799..43885
                     /locus_tag="ACHUR4_000415"
                     /pseudo
     CDS             43799..43885
                     /locus_tag="ACHUR4_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43974..44873)
                     /gene="yfeX"
                     /locus_tag="ACHUR4_000416"
     CDS             complement(43974..44873)
                     /gene="yfeX"
                     /locus_tag="ACHUR4_000416"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1454151.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44969..45544)
                     /locus_tag="ACHUR4_000417"
     CDS             complement(44969..45544)
                     /locus_tag="ACHUR4_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1454152.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45605..46054)
                     /locus_tag="ACHUR4_000418"
     CDS             complement(45605..46054)
                     /locus_tag="ACHUR4_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1454153.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46041..46466)
                     /locus_tag="ACHUR4_000419"
     CDS             complement(46041..46466)
                     /locus_tag="ACHUR4_000419"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1454154.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46680..47549
                     /gene="amiA"
                     /locus_tag="ACHUR4_000420"
     CDS             46680..47549
                     /gene="amiA"
                     /locus_tag="ACHUR4_000420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1454155.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47553..48452
                     /gene="hemF"
                     /locus_tag="ACHUR4_000421"
     CDS             47553..48452
                     /gene="hemF"
                     /locus_tag="ACHUR4_000421"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1454156.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48458..49510)
                     /gene="eutR"
                     /locus_tag="ACHUR4_000422"
                     /pseudo
     CDS             complement(48458..49510)
                     /gene="eutR"
                     /locus_tag="ACHUR4_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49556..50056)
                     /gene="eutK"
                     /locus_tag="ACHUR4_000423"
     CDS             complement(49556..50056)
                     /gene="eutK"
                     /locus_tag="ACHUR4_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1454157.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50069..50728)
                     /gene="eutL"
                     /locus_tag="ACHUR4_000424"
     CDS             complement(50069..50728)
                     /gene="eutL"
                     /locus_tag="ACHUR4_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1454158.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50738..50806)
                     /locus_tag="ACHUR4_000425"
                     /pseudo
     CDS             complement(50738..50806)
                     /locus_tag="ACHUR4_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51910..52701
                     /locus_tag="ACHUR4_000426"
     CDS             51910..52701
                     /locus_tag="ACHUR4_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454159.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53421..53816
                     /locus_tag="ACHUR4_000427"
     CDS             53421..53816
                     /locus_tag="ACHUR4_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1454160.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53834..54097
                     /locus_tag="ACHUR4_000428"
     CDS             53834..54097
                     /locus_tag="ACHUR4_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454161.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54120..54662
                     /locus_tag="ACHUR4_000429"
     CDS             54120..54662
                     /locus_tag="ACHUR4_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454162.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54801..55088
                     /locus_tag="ACHUR4_000430"
     CDS             54801..55088
                     /locus_tag="ACHUR4_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454163.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55127..55333
                     /locus_tag="ACHUR4_000431"
     CDS             55127..55333
                     /locus_tag="ACHUR4_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454164.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13854 a        14732 c        13728 g        13215 t
ORIGIN      
        1 ttttaatccc cttttaaatt tatgtttttt cctcaaaaac ggcctgctgg tcatcagcct
       61 tcaggttacc tatcatagag gtttaatcct tattcagagt cacccgtgca cgaaatattc
      121 aacatgctgc tggcggtctt cgatcgggca gcgttaatgc ttatctgcct gttctttctc
      181 atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg cgcactcccc aaaagaattg
      241 ctcgccgtta ccgccatttt ctcgctgttc gccctgttca gcacctggtc cggcgttccc
      301 gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga tgtccggcgg gattctgttt
      361 ggcccgtggg taggcatcat taccggcgtg attgcgggta ttcaccggta tttaattgat
      421 atcggcggcg tgacggcgat cccctgcttt atcaccagca ttctggcggg ttgtatatca
      481 ggctggataa acctgaaaat ccccaaagca cagcgctggc gctggatatc gccaacaaaa
      541 cgctaccgct gtttcgccat gtcaatagcg agtcattacg caaggtctgc gaaattatcc
      601 gcgatgacat tcacgccgat gcggtggcga ttactaatac cgatcatgtg ctggcctatg
      661 ttggcgtggg tgaacataac tatcagaatg gcgatgactt cattagcccg actacccgtc
      721 aggcgatgaa ttacggaaaa atcatcatta aaaacaatga tgaagcccac cgcacaccag
      781 agattcattc catgctggtg atcccattgt gggagaaagg ggtcgtgacc ggaacgctga
      841 aaatttacta ctgccacgcg catcagatca cctcgtcatt acaggaaatg gcggtcggtc
      901 tgtcgcaaat catctccacg caactggagg tttcacgcgc cgagcagcta cgtgaaatgg
      961 caaataaggc agagattcgc gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg
     1021 ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga taccgctcgc cagttgatct
     1081 ttaatctgtc gcgttatctg cgctataaca ttgaattaaa agacgatgag caaatcgata
     1141 tcaaaaaaga gctgtatcaa attaaagact atattgccat tgagcaggcc cgctttggcg
     1201 acaagctgac ggttatctat gatattgatg aagaggtgaa ttgctgcatt cccagcctgc
     1261 tgatccagcc gttggtggag aacgccattg tccacggtat tcagccttgc aaaggtaaag
     1321 gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt acgcattgcg gtgcgagata
     1381 ccgggcacgg catcgttcca aaggtgattg agcgggtcga agcgaatgaa atgccgggca
     1441 ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt attgtatggc gaggggctgc
     1501 atatccgccg cctggagccg gggacggaaa ttgcgtttta cattcctaac caacgcaccc
     1561 cagtcgcctc acaggctacg ttattgcttt gagccggagt gatattgtga aagtcatcat
     1621 tgttgaagac gaattcctgg cacaacagga actgagctgg ctaattaaag agcacagcca
     1681 gatggagatt gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa
     1741 ccgcgtcgac gccatttttc tggatatcaa tattccgtcg ctggatggcg tgttgctggc
     1801 gcaaaacatc agccagttcg cccataaacc gtttattgtg ttcatcaccg cgtggaaaga
     1861 acatgcggta gaagcgtttg aactggaggc gtttgactac attctcaaac cgtatcagga
     1921 gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc tggcaacaac agcagaccag
     1981 cagtacgcct gccgcgacgg taacgcgtga gaatgacacc attaatctgg tgaaagatga
     2041 gcgaataatc gtcacgccaa ttaacgatat ctattacgcc gaagcgcacg agaaaatgac
     2101 ctttgtctat acgcggcgtg aatcctacgt aatgccgatg aacattaccg aattttgcag
     2161 caaactgccg ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca atctgaacaa
     2221 aatacgcgaa atcgaaccgt ggtttaataa cacctacatt ctgcgactga aagatctgga
     2281 ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt cgccagttaa tgcatcttta
     2341 atcgggaact ttcatgaaag caccaggctt gcccgccgat caacaatttt tcgccgatct
     2401 gttcagcggc ctagtgctta acccgcaact actggggcgc gtctggtttg ccagccagcc
     2461 tgcctcgtta ccggtgggca gtttatgtat tgattttccc cgtctggata tcgtgctgcg
     2521 cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga atggtggaag gagaaatgct
     2581 gtttattccg gcacgcgcgg ctaatttacc gatcaacaac aaaccggtga tgctgttaag
     2641 cctggtgttc gcaccgacct ggcttgggtt atcgttttac gatagccgca ccacgtcgtt
     2701 gttgcatcct gctcgccaga cccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat
     2761 gcttaccgcc ctcacccatc ttagccgttc gccgctggag cagaatatca ttcagccact
     2821 ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat atgccgccgg gtaattcaca
     2881 accgcgcggc gattttctct atcacagcat ttgtaactgg gtttaggata attatgccca
     2941 gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa
     3001 actgtttgct cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc
     3061 gaaggcgagg atgattttgc agaaatatca tctgtcaatt catgaagtgg cacagcgttg
     3121 cggttttccg gatagcgact atttttgtcg cgttttccgg cgtcagttta gtctgacgcc
     3181 gggagagtac agcgcccgtt ttcagggcta acgtcagaag gttaattctg tttccagcag
     3241 cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt ttttattcac
     3301 cagtttacgc gccagctgcg agaagacttt cacatgattc atcccttcgt tagcacccag
     3361 cgtcagcatg atcaccagtt cgacttcgcc catttctgac tgccagtcaa ccggtttcgc
     3421 cagccgggca atgctgatac tggaatgacg gatccactga gatttggtgt gcgggatcgc
     3481 tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc tgccagacat cttcttccag
     3541 ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg ccgcacagga actggatcgc
     3601 ctgctcttta ttgctaaaat cctgatcaac aaagatattc tccagcgcca gcagtgggcg
     3661 aacgtcttct tccggcgtaa aggcggtgag taacgcttca atttcctgcg cactgcggca
     3721 ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta tccagttgac gaagctggct
     3781 tttcaccgcc ggaatacgcg ggctactcat actcagctca tccaggccca gcccaagcag
     3841 tagcggcaga taacggcttt caccgcccag ttcaccgcaa atgcctaccc atttgccccg
     3901 ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg aacgatggcg taatcgggtt
     3961 atatagcggc gatacgcgcg ggttattacg atcgaccgca tacagatact gggtcatatc
     4021 gttggagccg atactgaaga aatcgacctc atcgcagaag tggtcgatga tgtagcacac
     4081 cgacggcact tccaccatga tcccaagcgt aatcgtctct gcatgacgca ggccatcgcg
     4141 cttaagctca acgatcgctt tttggatctc gcctttcacc cataagatct gatcgaggct
     4201 gtgaaccatc gggatcatca actgggcgtt gccgaaactg gcggcgcgca aaatggcccg
     4261 cagttgagtg cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc gatagccgag
     4321 gaacgggttc tcttcctggg gaatattcag ataaggaatg cttttatcgc cgccgatgtc
     4381 catcgtgcgg aagataatcg gcttgtcgcc cgccgcccgc cgccagtagc acctgctggt
     4441 aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag agcatttcgg
     4501 tacggaacag cccgacacct tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg
     4561 tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc agctgcgcgg
     4621 cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc tgataataac
     4681 cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca agcactgcag
     4741 gttgcccggc ataacgggca atcgcatcca gcggcaagcc actcagtacc gggatcgccg
     4801 aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt tttctccaga atcatacccg
     4861 caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca gccaccagaa
     4921 tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag gtgatatgca
     4981 gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag
     5041 aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc cccagtccct
     5101 gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg
     5161 acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat tgctgattca
     5221 gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc gcactggcgg
     5281 ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta cccacgccga
     5341 cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt aatgaacgcg
     5401 gcagcggatg cgccgtcagt tctgcctgcg tcggctgaac gctgtcgctg tcgataaagc
     5461 gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt
     5521 tcaggctgca actgtcatta aataaggtgc cagttccaat caacgccagc gagcttttag
     5581 catcagcctt tgcgttctgg cgatggttga taaaggtgat ttcgctttgc cactggctgc
     5641 actgttcttt aagttcccac gccggacgag catgtagacc gttaggcaaa ggacagagaa
     5701 attgaatcgt taacataacc gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt
     5761 gaacaaccgt ctcacgcgta agacgttgaa taagggcaga taaaagttgc tccatctgca
     5821 aaatgtcgcg gcaatcggca atcggcaatc gatgccgcgc aatgtccgtg gcgggtggca
     5881 ggccccatta ccaatgtggg tacgccagta ccggttaagt gcaccgctcc accgtccgtg
     5941 ccgccgttac tgaacatatc cgcctgcaac ggaacgccaa tttctgccgc cacggtttca
     6001 atccacgcgg taagttttgg cggcgcaatc agcgatttat cgcttaacac cagcatcggc
     6061 ccgttaccaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg
     6121 gtatcaagca caatggcgac gtccggcgac accgcgcggg tagcagtttg cccgccgcgt
     6181 aatcccacct cttcgctgga acttgccacc agccacactt ccgcaggcag ttcggcgtcg
     6241 tgcagctcac gtagtaacgt caccagcaga tagcaactga ggcgatcatc aaaggctttg
     6301 cccatcaccc gctggtgagg gaggacctga aaagtggtat cgaaggtgac gcgatcgcct
     6361 ggacgaattc ccgcctgcat cacttcgtca taggtgcgcg cgccaatgtc cacgcgcatg
     6421 gcgctaacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
     6481 cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc
     6541 agcacgtcaa tagcgccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
     6601 tgggcgcaga tcatcacctt cggacctgtc gattcattga ggcggatcag caccgatccc
     6661 agaccatcaa atcgcacttc tttttgcagg cgagccgctt cttccagcag gatctgccgc
     6721 acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa
     6781 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
     6841 cttgcggggt aaccagcaca acatcttcga tgcgcacgcc cccttgccct agcaaataaa
     6901 tccccggctc cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa
     6961 aacgcggatc ttcatggact tcaatgccga tagcgtgacc agtgttatga ccgaaatagt
     7021 cgccaaatcc agcctcggta atgacccggc gcgcggcgtc gtcaatctgc tggcagcgca
     7081 cgccggggcg aattgcagag attgccgcga gctgtgcctg caggacaatc tgatagacgt
     7141 caaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
     7201 tatcagcagt agccctgata cagcgcacca aaatcgagag tgacaaactc gcccgctgca
     7261 acaatcttgt cgctggcttt gccgtgcggc agcgccccgc gccagccgct ggcgacaatg
     7321 gtgtcaaaag aggctttttc tgcgccctgc tggcgcataa accactccag ttcagcggct
     7381 atctcgcgct cgctcatccc cgcctgaata aagcggcgaa tatgctccgc tccgcgatcg
     7441 gcaatcccac aggcgaggcg gattatctcc acctcctctg gcgttttgat ttgccgcagc
     7501 acatccggcg tggcgctaac cagtttcgca ttgagttcag acttccagcg gtgcgcggtt
     7561 tcccaactca cctgctggcc ttcaaaaccg agcgtttgca actgctcatc ggcaatgatt
     7621 tgattgacga tagtggtaag cgtgttcgtt gcgtcaagca aatgcagctg gtagccttgc
     7681 gcacgggctt ccacctcaac gaaatagcgc gaatctacca gaatgtgcgc actttcacgg
     7741 ctaataacca catagcctga tccggtggag atccccagat gcggctgttt gttctgccgt
     7801 gaggagagaa gcaccgcatc cagttgttgc accttaagcc agttgcgcag cgaagcgagt
     7861 aatgtcatct ggattttcct tcttacaggc tatcgattaa caatttgcct ttgcggaaca
     7921 tcatcagacg caggaacacc accatcagcg cagtaatgat tgcccccagc gcgatccccg
     7981 ccatataaac gcccaagtta gtgaccagcg gccatgccca gatagcggat tccgggaacc
     8041 attgcactgc gcccagccag acagctgcgg ttgagccgac aatcgcgccg accatatatg
     8101 acggaatagc ggtgatgggg ctttccagcg caaacggaat cgcaccctca ctaatcccca
     8161 taaaggccag gaacatcgcg gtcttaccct gcggataaag ttgagcgttg aacaggcgtt
     8221 tgccggttaa acgacggtca ataatggtcg ccagacccag accaatcggc ggaataacga
     8281 tagcgataga acgagcggtg accggtaata cgtggtcagt ggtaaagctg aaggcaacga
     8341 aacctgcggc tttgttgatc gggccaccaa ggtcgattgc tgtcgccgcg gcgatcccca
     8401 ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag cacagtacgg ataccgccgt
     8461 tgatccagcc accaaaggga gtgatgacgt agtacatcgc cagcatgaca aaaatggcgg
     8521 aaagaatcgg tagcaaaaat gtggttttga acgctaacag gaaatccggt aactggattt
     8581 tctggttcat ccatttcaca aggtaaccgg caacgataga aataatcagc gcaccaatga
     8641 aggtggacgg caccggcgaa gaggtcgccc attgcatagt gctgggatcg aagttcagca
     8701 gttgcgtcgg ctgggtagac atcaacccgc cgataaaacc agccgggaac gccagtttgc
     8761 caccgataga gttcgccaca aatgcagcaa acatcggaat ggcaaaacca aacaacacgc
     8821 cgccgaagga ctgcgacagc caggcaaatt taagtaagga gaggtcgaaa ccggagaatt
     8881 tcccgctatt aagagcatcc atgatgccga tctcagcagg aattttaagc cagctataag
     8941 caatcagctg gctgaaagcg aggatcacac cgcccataat taaggtcggc accatgcgtg
     9001 aaatccctga catcacatgc tgcgggagtt caccccagaa gctgctttta gaggcctgcg
     9061 gattcttaac tgccgctgcc gcaccggatg cgccaggcac aacggttgca ctgcgttttt
     9121 taatggccat aacaattatc cctgtcgaat attactgttg ttcagaggca atcatctctt
     9181 cgatttcttt gatgatgccc gcagcgtttt taattgcgtc ctgcaaagtg atttcataaa
     9241 cgtcgcgtga ttcgaaacgt tcgttatctt ccggggtaac tgccacggaa tggatgatga
     9301 tggtcgcttc ggcgatatcc tgcgccgtca ggcgattctg gatgccgtcc gcgccctggg
     9361 tttcaatttt cacttcataa ccggcttcta ccgccgcttc ttccagcacc tgagcggcca
     9421 taaaagtgtg agccaggccc attgggcagg cacataaggc aatcagtttc ttactcatcg
     9481 ttaattcctc gttcagtgat agttgactag tgaaattatg aacgtgcaaa acaaatcgcc
     9541 gttaccagac aaataatgca tttactggag aattaatccc gctgtacgga agtgtgacgc
     9601 aggtcggcct tgtgagggga cacaaagaaa aattgaatta actgtatgat ttaaaagatt
     9661 atcgggagag ttacctcccg atataacagg aaggatttac agaatgtgac ctaaggtctg
     9721 gcgtaaatgt gcaccggaac cgagaagacc cggatagtca tggacgatga gatacaccgg
     9781 aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac ggaaaccgga
     9841 ggctttgaag aactcaagga agcgcggcac gataccgccc gcaataaaca cgccgccaaa
     9901 tgtcccgaga ttgagcgcca gattgccgcc aaaacggccc ataatgacgc aaaacagcga
     9961 caatgcgcgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa tatcttttgg
    10021 cttgagattt tctggcaggc ggttgtcagc tttcacaatt gcgcgataca aattcaccag
    10081 cccagggcca gaaagcacgc gctccgccga aacatgacca atttccgcac gcaatatttc
    10141 gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc cttcgcccgg
    10201 caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc ccgttccggc
    10261 accgtaaacc gcaataggct taccttcgac cggttctgcg ccaccaaact gaatcagatg
    10321 ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa taatttccag
    10381 atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg tatggttggt
    10441 cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt ctttcacctc
    10501 gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt aatcaagccc
    10561 tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag caagacgtgc
    10621 gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac tgctccgcta
    10681 aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac aacgccggaa
    10741 aggcaggctc cctgtaaata tcgatctggg tcacacaatt actttatcgt ttcagcacca
    10801 attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg cataaggaat
    10861 agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc gccgtggcaa
    10921 ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg gtattacaga
    10981 atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca cccctctgga
    11041 tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt ttcagccagg
    11101 gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccggtt ccgccctctg
    11161 cgctacctag acttatcgta gcattaattc tcggtcttgc tggcggcgtc agcctggggc
    11221 cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc gctcgtctgt
    11281 taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc atcggcgcgc
    11341 tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat ggcagtagtg
    11401 aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct ggtgcactta
    11461 ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac ggacagatgg
    11521 aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca gcagggatgg
    11581 tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa aatccggtgc
    11641 tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt ggaccagttt
    11701 cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct ttcagcacca
    11761 gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct gccgccagtg
    11821 gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta gggttgatgc
    11881 tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct attctcggca
    11941 tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca gtcgttgtac
    12001 ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg ctgttattag
    12061 caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac gataatgttt
    12121 agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc
    12181 cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt
    12241 tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaatgcag
    12301 gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat cattacggta
    12361 attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat tatgaatggc
    12421 gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat aagcgtaaag
    12481 catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc
    12541 gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc tgatgcgacg
    12601 ctagcgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg ataaggcgtt
    12661 tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat cccagcgccg
    12721 tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc cagcctgtct
    12781 gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg gtgaaaatca
    12841 gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc ataaccagaa
    12901 tccgtgtcgg atctaatccc atcagaatga caataaatga cggcaacatg gtgactgtac
    12961 gacgcaccca cagcgggata tggaagcgaa tgaatccctg catcaccacc tgccccgcca
    13021 gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca aagaccgttg
    13081 ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga tcggcaacac
    13141 cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcacc agattgacaa
    13201 aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa cgttgttgac
    13261 gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca tgcggcataa
    13321 tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt aaactcggga
    13381 tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac tcgacaatgt
    13441 aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagcggt ttttgcccgc
    13501 gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg ccctgcaaca
    13561 acgaaacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc gccaggtcgg
    13621 ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga cgcggatagt
    13681 gatcacgaat ctgctccgcc agatttttac cggtggcaat ccctagtttg gcagagagga
    13741 tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc tgatagccga
    13801 agctagcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa ccaatcgccg
    13861 caatgaacgc aggtcccatt aatgcgagcc tcatcttacg cgccgcccgt ccgctgctac
    13921 tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct atgtttcatg
    13981 ctatgctaaa cgagaatgat tatcaaattc atttaaatgg attgtggtga tttctctgat
    14041 agaccaagat tatgattacg aaaagattga tgacttgaat gttatggtta tgtttaatgt
    14101 tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt atgacagatc
    14161 actatttttg aagcctgtca caggacgtca ttatagtgtg tgtcagatct cgttttcctt
    14221 aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg agcaaatatg
    14281 gaccgcgtcc ttcattttgt actggcactt gccgttgttg tgattctcgc actgctggta
    14341 agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt tatcgaagtg
    14401 ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa aggcttctcc
    14461 gaaatgttcg aaaaactgct cggatttgcc aacgaaggga ctaacttcgt ctttggtagc
    14521 atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat cgtctttatc
    14581 tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat ccgcgcaatt
    14641 ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa cgccgtcagc
    14701 tccctgattc tgggtcagtc agaaaacttt attgcctata aagatatcct cggcaaaatc
    14761 tcccgtaatc gtatgtacac catggctgcc acggcaatgt ccaccgtgtc gatgtccatc
    14821 gttggtgcat acatgaccat gctggaaccg aaatacgtcg ttgctgcgct ggtactgaac
    14881 atgttcagca cctttatcgt gctgtcgctg atcaatcctt accgtgttga tgccagtgaa
    14941 gaaaacatcc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat gctgggtgaa
    15001 tacattctgg caggtttcaa agttgccatt atcgttgccg cgatgctgat cggctttatc
    15061 gccctgatcg ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt tggctacagc
    15121 atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt gatgggtgtt
    15181 ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt ttccaacgag
    15241 ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg tgctgaaggc
    15301 atcatctctg tgttcctagt ttccttcgct aacttctctt caatcgggat tatcgcaggt
    15361 gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg tctgaaactg
    15421 gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact ggtgctgtaa
    15481 aaccatacat aaaaaagccg gggatcattc cccggctttt ttacgcctgt taatcagcta
    15541 atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc ttatgcaata
    15601 gcgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac ttcgctttcg
    15661 ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca atatctttca
    15721 caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg
    15781 catatccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc agctgctcga
    15841 tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga
    15901 ccgcctgcgg ggagatgtga taacgcttaa acagacgaat aatccgcccg gcaatattct
    15961 tttgcagcag cgtgagcggc attaaattga ctgaaaagcg cggtcctttt ttgtcgcaag
    16021 ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa cgcgcgctaa
    16081 ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg ccgtcatatt
    16141 tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt ggctgggcgt
    16201 agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat agcacctgtt
    16261 tagtggtctg cccggaaacc atctcctctc tgctatccag cgccagcaca tgatgatgtg
    16321 cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg gtttcctgat
    16381 ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta ttgttccagt
    16441 gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc gtttccggcc
    16501 cgctcagcac taacagcagt tcactacccg gcaattgata caacttttcg ttttcctgca
    16561 tcaacggcag cagcgtacgg caaattgagc ggatacagtg aacgcgcatc attaagccgt
    16621 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct
    16681 cttgctccgg cgcttgttcc aacgcacgaa agttgggtag gagcgttaac ggatctgtta
    16741 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    16801 agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg
    16861 tttccacccc ttgcaaaaag ttctgattgt aattcagaag gcaaagcgtt gaaacagccc
    16921 aggtgagatt taaaaacgga tagcgaagat taccgacccc gagggtaaag atgatgaaga
    16981 aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac aacagaagta
    17041 gcagcacgct taacgctgcc agccagctta aggtaaatgc gagtgagata gtagaaaagc
    17101 atgttgtaaa tcagcactgc ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca
    17161 tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga
    17221 tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag
    17281 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    17341 caggcacaca acacgacaaa cgtctggctg ccgagcatga ccgcagtctg taaaaaagag
    17401 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc
    17461 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    17521 taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    17581 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    17641 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    17701 atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat gctttcacca
    17761 ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    17821 cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac
    17881 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    17941 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    18001 gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag
    18061 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcaacgtgta
    18121 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    18181 gttattcatg tcacggtttc ctgtaaagtg gtgttataaa atgaactact aatagaccca
    18241 catacattca gggaattgtt atgttcaagg agaggatgac accagatgaa cttgccagac
    18301 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt
    18361 caccaaaacc tggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc
    18421 gtgaatatat tcgcaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg
    18481 gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac
    18541 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    18601 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    18661 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggaccagaga aaaaggctgg
    18721 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    18781 caagtttgcg agtttattca gaacacgcca gccttccata acacaccaat gttgatggaa
    18841 gctgaagaga gaattgctga atacgctcct ggagcacgcg ccccggcgta tcgacaaatc
    18901 atcaacgcga tagataatat gacagacatt gagcaggaaa aagtcgcgca atttttgtca
    18961 cgtgaaggaa ttcgcaattt cctcgcccgt ctcgatattg acgaatcagc ataaataaaa
    19021 aacggcagga tattatctcc tgccgtttat ctttttacac gctaattact gctgattttc
    19081 gcgttcagca ataaaatcca gcgctttgtt gatacgctcg atactgcggg tcttaccaat
    19141 tgcgtgaacg gtaacatcca gtgctggaga ctgccccgca ccggttacgg cgacacgcag
    19201 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    19261 atgaacgttt tcagcggtcc agtcagtaat cgcggccagt ttgtcacgaa ccacttccag
    19321 cggctgacgc gctaccggac gcagatgttt tttcgccgca tcggcatcga actcagcaaa
    19381 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    19441 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    19501 aatgtgccac tgtaagtgag tagcaacata ctccggcggc agcgcgttaa tgtagtgatg
    19561 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcaa
    19621 agtgaagtat ttgatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    19681 cagacgcacc agatagttca gcagtgcttc tggcaaataa ccgtcatcac gatactgcat
    19741 tacgctgact gccccgtgac gtttggacag ttttttaccg tcatcgccat tgatcataga
    19801 aacgtgcgcg taaaccggca ccggcgcttt cagggcctta agaatgttga tctggcgcgg
    19861 cgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    19921 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa taagatcgtc
    19981 cagttcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    20041 ttcctgcggg ttagcaaaac gtacaacaca cggttcatca tcagcatgat gctcatggct
    20101 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    20161 cgcttccagg cgctctttag agcaatagca tttataagca gtgccctctt ccagcatctg
    20221 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    20281 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    20341 ctcaagatcg gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag
    20401 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    20461 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    20521 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    20581 tcgggtttct accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    20641 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    20701 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    20761 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccaactac
    20821 tttatgtagt ctccgccgtg tagcaagaaa ttgagaagtg ggtgattagc tcagctggga
    20881 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccacttcg
    20941 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    21001 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac
    21061 tcaaagattc gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg
    21121 cggcgacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgcaaaaa
    21181 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    21241 ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta
    21301 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    21361 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa
    21421 ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga
    21481 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaaa cagcaggcag
    21541 aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    21601 aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc cgaccagcca
    21661 ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    21721 aacggttctt cctgcgcacc agcatcaact ggcgttcaac caccggcgta atgcgcttaa
    21781 ccaccagtgg gctaccttca ggcaatggta gcgccagtgc aggaagaata ctgatgccga
    21841 tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg
    21901 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat
    21961 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca
    22021 aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca
    22081 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    22141 tttccatcac ccactgctgc ggacgatcat gcaggacaaa ctgaatatct ggatagcggc
    22201 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    22261 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    22321 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    22381 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    22441 tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct
    22501 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    22561 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    22621 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    22681 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    22741 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    22801 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    22861 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    22921 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    22981 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    23041 gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    23101 cacggtgcaa ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    23161 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    23221 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    23281 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    23341 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    23401 agcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    23461 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    23521 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    23581 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    23641 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    23701 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    23761 tactcctccg gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    23821 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    23881 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    23941 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    24001 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    24061 cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    24121 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    24181 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    24241 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    24301 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    24361 cgctttttcc agcgcgttaa ccacgttttg tgccaatttt ggccccatac gctccagtcc
    24421 ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtatggacat attctttttc
    24481 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    24541 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    24601 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa
    24661 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    24721 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    24781 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc
    24841 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    24901 aaaggtcatc tgctcctgcg ccgggaattt aaacgcaacc gcccagcgcg gggcacgcgc
    24961 gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat
    25021 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    25081 cgccgattca caaagcgtta cccgatcgct gaccggcacc ccccactttt taaattgcag
    25141 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    25201 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    25261 tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat
    25321 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    25381 attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc
    25441 ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag
    25501 aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt
    25561 tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt
    25621 atccagtgac agcattggta cttcatggcg tatctggctg aaagctgcca gcggcgcagc
    25681 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    25741 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    25801 atgataaaga tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    25861 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    25921 ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt
    25981 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    26041 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    26101 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    26161 gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat
    26221 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    26281 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    26341 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    26401 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    26461 ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    26521 gttcaggctg cggctgatag gcaggctgct gcaccggctg cgctggacgc ggagcatgtt
    26581 gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat
    26641 aaggcggtag gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag
    26701 ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat
    26761 cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga
    26821 acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg
    26881 cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg
    26941 ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc
    27001 aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct
    27061 gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt
    27121 ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc
    27181 cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc
    27241 ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt
    27301 ggctctttac acagctcgat gtctggatcc cgactttcat gagttacgtt ccggactggc
    27361 tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg
    27421 gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg
    27481 aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca
    27541 tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgtctgg tatctgccgc
    27601 gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg
    27661 tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg
    27721 ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca
    27781 atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca
    27841 tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac
    27901 acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg
    27961 tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata
    28021 tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt
    28081 tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt
    28141 cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa
    28201 tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc
    28261 gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc
    28321 gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca
    28381 gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg
    28441 acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc
    28501 aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac
    28561 gaaaaaacca ccggtccgga gatctgggaa gataccgacg gtcaggttga tgtatttatt
    28621 gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa
    28681 ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag
    28741 gtgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt
    28801 tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa
    28861 gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct
    28921 tctggagcag ctgttgccgc agcgttgaaa ctacaagaag atgaaagctt taccaacaag
    28981 aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc
    29041 gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa
    29101 agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga
    29161 ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg
    29221 ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg
    29281 cataatgttt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat
    29341 tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga
    29401 accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt
    29461 tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt
    29521 ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg
    29581 ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga
    29641 ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg
    29701 cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat
    29761 gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga
    29821 agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga
    29881 acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc
    29941 tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga
    30001 agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga
    30061 cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga
    30121 atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat
    30181 cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc
    30241 tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac
    30301 cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc
    30361 tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga
    30421 tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc
    30481 tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc
    30541 tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt
    30601 tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt
    30661 catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc
    30721 tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga
    30781 gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg
    30841 tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc
    30901 ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc
    30961 actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga
    31021 cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca
    31081 tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct
    31141 ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt
    31201 gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat
    31261 gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga
    31321 cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa
    31381 cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt
    31441 aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg
    31501 cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt
    31561 ccgacgacaa aaaggatacc ggaactattg agatcattgc tccgctctct ggcgagatcg
    31621 tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg
    31681 ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct
    31741 ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact
    31801 tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc
    31861 agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag
    31921 ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca
    31981 aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct
    32041 tgccgcagtg aaaaatggcg cccatcggcg ccatttttta tgcttccgcc agcggcggca
    32101 aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt acgcgcaacc
    32161 ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg ataagctgag
    32221 cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg gaaatgacat
    32281 tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca ttaccggagg
    32341 cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg
    32401 cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag atattggggg
    32461 taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga
    32521 cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat aggtcaggat
    32581 ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat gccgttccca
    32641 tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga agcgcacgca
    32701 aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca taatgcggcg
    32761 tattgctcag caataccgtc ggcacggcaa agacattcaa gccgttctgt tttatagcag
    32821 gcaccgcaat gctgttgcct acgcttccgt acactacctg cgactgcacg gcgacgatat
    32881 ccgcctgcag cgccctactc ttatcgttaa acaacaacaa actactcatt taattttttc
    32941 tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct ggcagtcaaa
    33001 tcggctatta caaaacaaag ataaggtaat tcagtgaaga aaatcatttg tctggccatt
    33061 acgctgctaa tgacactccc cgcttacgcg aagttaactg ttcatgaaga agcccgcatc
    33121 aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg caacggtgat
    33181 gaacatacct gcgatgaagc ggtttcccac ctgcgtctga agctcggcaa tacccgtaac
    33241 cgcattgaca ctgccgagca gtttattgat aaggtagctt cgtcgtcatc gattaccggg
    33301 aagccgtata tcgtgaagat ccccggtaag agcgatgaga acgcacagcc ttttttacat
    33361 gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc cgctttggta
    33421 aggcatttac aggaggtaac atgaaaaaat gctttattta tcacgatgaa aaatcgaata
    33481 aattttggtg gatagattac gaaggggata gtttagctgt caactatggc aaggtaggta
    33541 gtattggtaa attccataca aaagaattcg ataatgaaga acagtgtctg aaagaagcca
    33601 gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga aactccagac tttaacttca
    33661 tggatctcta ctattttgat gatgaagaga ttgggttaca tcttaaaacg tcacacccaa
    33721 acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa gaatctcctt
    33781 ttggtagcga tgaaggtgct gatgctctaa acgaacttga aaatagcctc cgttctggac
    33841 tgtgctgatt tccctcaaat gttaattgaa actatgtggg gtatgaaata catcgctatg
    33901 gacagtattc ttgaagagga tgtgcgtgcg caattactag ccgatgaaat gagctctatc
    33961 cagagcaata tgattaccta cgcgactgca ttcggtcaga tcaaagtcat gggtaaaatc
    34021 tcccataaac ttaaaaagat gggactcaat gcactagcgc gtcatcagct taccgcaaaa
    34081 attcttcaat ggggtgacgg tcaggactca ccaatacttc aaaaaatgat tgatgacctt
    34141 actgcttttc cacacgaaaa ttaaaaactg catttgtcgg tagcaacaac tgccgacaaa
    34201 tcagattatt gacaaagtgc actttgttta tgccggatgc ggcgtaaacg cctgatccgg
    34261 cataaaaata ttgctaattc aatatattgc agaaacattg taggcctgat aagcggagcg
    34321 catcaggcag ttttgcgttt gtcatcagtc tccaatgcta ttaatcctta aatccctgcc
    34381 ccctgactaa aatgctcttc cccaaacact ccggtggaga ggtagcgatc gccacgatcg
    34441 cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag tgctccagca
    34501 accgcgccgc ctgagctgac gccacagaat attccttccc gcaccgccag ttcgcgcatg
    34561 gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagcgc agcgttgaaa
    34621 atccccggca gatattccgc aggccagcgg cgaatgcccg gaatactgct gccttcttcc
    34681 ggttgcaggc caacaatggt caccggtttg gattgttcgc gcataaagca tgaaacgccg
    34741 gtgatagtgc cggtcgtccc catgctggag acaaaatgag tgatgcgccc gccggtttgc
    34801 tgccagattt ccggcccggt ggtggtgtaa tgcgcataag ggttatcggg attattgaac
    34861 tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc gcgcgcacct
    34921 tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg catcgccgca
    34981 cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc tttcagcgcg
    35041 gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa gacatcaccc
    35101 ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc acgatctttc
    35161 accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc gttatccggc
    35221 cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa tgtactcaca
    35281 atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag
    35341 gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg cgtttaacat
    35401 tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatcgcggg tttcaatacg
    35461 ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct cgccatgctg
    35521 cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg
    35581 caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta ccggcagcgg
    35641 cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga agagatccac
    35701 cggcccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa catggaactg
    35761 cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga gtacaaaacg
    35821 ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat tcccctgact
    35881 catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg tcacaaacac
    35941 gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct ctttacgcac
    36001 ctgcgcatcc agcgcgccga acggttcatc aagcagcaga atttgcggtt ctacagccag
    36061 cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa agctgcgccg gataacgatc
    36121 cgccagatgc gcaagctgga ccatttccag caattttgtc actttcgctt tgatggctgc
    36181 ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagtgatat tgtcgaatac
    36241 cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac gatcgcgcgc
    36301 gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt gatgctccag
    36361 cccggcgata atccgcagca gcgtggtttt cccggaaccg gacggcccca gcaacgcgac
    36421 catctgacca gaaggaatat ccagtgagat atcgttcagc acctgggtgc gaccaaaaga
    36481 cttcttgata ttggcaatct caatgctcat gatgctcctc ctgctgcgcg cgtttttcct
    36541 gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc gccatcaacg
    36601 tcaacagcgc ggcggcggta aaggagccga cggtgttgta gtcctgctcc aacaattcaa
    36661 tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc ggaaaccacc gacactgcac
    36721 caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc gtaaagcagc gcccagcgaa
    36781 tattcggcaa tgtgacgcga cggaacatct gccagccgga cgcgccaagc aaaatcgccg
    36841 cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc accacaaacg
    36901 gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata atttgcagat
    36961 tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc agatacacca
    37021 gaccggcaac caccggcgat acagcaaacg gaatgtccag tagcgtcagc agtaactggc
    37081 gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac accaggttta
    37141 ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc atgtccggat
    37201 cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg aagatgtaaa
    37261 tcatcggcac cagcaggatg aacgccgaaa ccagcatccc gatgccaatc agaaaccatt
    37321 tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc gccattaatg
    37381 acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca gcagcagcag
    37441 agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc gggtaatcaa actcctgtaa
    37501 gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac gcgatatttc cggcgataaa
    37561 aatcaccgcg ccaaattcac caagactacg ggtaaacgac agcgccacgc ccgccaccag
    37621 cgccggagaa agctccggca gcaccacttt gcagaaactc tgccagcgcg ttgcaccaag
    37681 cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct gcacggtacg
    37741 caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc aggtataggt
    37801 gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg aaaagagcga
    37861 ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc
    37921 aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca gcagaccgaa
    37981 aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt aggccgcgac
    38041 cacctgcggg ttggtgatca cctcccagta ctgcgcccag ctcatctcag aaagctgcat
    38101 caccagcgca gagagcggca gcagcaatat caggcacaca aacagcagac tggtgccaag
    38161 gcttaaagta aagcccggca gcacgcgtct tgaagaaact gcaaacatta cttacgcccc
    38221 gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat cacttccggc
    38281 catgagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt atctttcagt
    38341 ttgtccatga cttccgggtt attcacacgg tagtaatagt cggtgatgat ggtctgcgcc
    38401 tgtgggctgt agagccagtt caggtaggct tttgcggctt tttccgtacc gttggcctgc
    38461 acatttttat cgacccacgc caccgggaat tccgccagaa tattggtttt cgggatcacc
    38521 acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga ttcaaagcta
    38581 atcagcacat cgcccaagcc acgctcggca aaagtggtgg ttgcgccacg accgccggta
    38641 tcgaacactt caacgttttt caggaactgg gtcataaact gttcggtttt ggctttatcg
    38701 ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg cgcgttaccc
    38761 gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc gttccagtcg
    38821 tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta gaacggcgag
    38881 ctattattcg gcaggcgcga ctgccagtca gccgggatca gcttgccttt gtcgtgcagg
    38941 atttgtacgt cggtcacctg gttataagtg acaacgtctg cttttaagcc ctgcaaaatc
    39001 gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt gtcgccgccg
    39061 ttatcttttg cccattgctg ttcaaacggc ggattcaggg cggcaaacag ctcgcgggag
    39121 acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag cagcagagaa
    39181 gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca cccttataaa
    39241 tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt aacggtttta
    39301 tataccgttt ggtgatttgg aagttaaaaa gggaataaga acttggttca acaggcgtaa
    39361 ttgttgcagg cagtttggac acggacagcg cggagaaacc ggagcgtaca catagtacgt
    39421 gaggatttcg agcactgccc ggggccaaaa tgacaaatga aatagcctgg tgaacttagt
    39481 tcaagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg
    39541 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    39601 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    39661 cgacacagcg ggattgcttt tgccatttca gtcagcaccg attctggatc ttccgggttc
    39721 gactggcggg caatgctttc cgccattggc gtgcggacgt atcccgggca gatggcgtta
    39781 acgcgaatac cggattgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    39841 gctttcgtta aggcatacgc cgtttcgcca ggatcagcca ccatatcacc agtgactgaa
    39901 gacatcatca caatgcgacc atctttgcgc gcaatcatct ccggcagcac cgccttcgtg
    39961 acgttccata cgcctttaat attgatatca atatggaaat cgcgatcttc atcgctcata
    40021 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    40081 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    40141 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgctagctt ttcgatctca
    40201 ggggagatat ccagcaagat taggttcgcg ccatgacgtg caaaagttct ggcaattcct
    40261 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    40321 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    40381 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    40441 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgacg
    40501 ccacatcatt gatgttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc
    40561 gtgcgggtag acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg
    40621 aaatgcgcca gccgccgcag cggagagtca gccagagaag taatggcaat caccgttgcc
    40681 ccctgctttc gtgccgcttc tgcgcacaag acaatctctt ttttactgcc gctgtaagag
    40741 atagcgattt gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg
    40801 tcggcttcgc aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc
    40861 accagcgccg atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca
    40921 atgatttttt gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct
    40981 ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg
    41041 tgcaggtgca gcgccgtggc attcgttttc tcccggctgg cactgtattc gccaattaac
    41101 gccatacgca attcagtaaa tccttgcgcg ccgagcttct gggcaaattt cacaatgctc
    41161 gactggctga taccgagctg tttcgccatc tgccgtgaag agacggattt cagctcgctc
    41221 actctggccc gcagaaagtc agcaattttt tgctcgttcc cggtaaattc actttccgca
    41281 ttgcgtattt tcgtcaggta caacatcttc cactctccta ggctggcagc aaaaattccg
    41341 cttaagttct tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt
    41401 ctgtcaattc gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg
    41461 tcaccaccga tgcaactaga aaagatgatt actgaaggct cgaacaccgc ctcggctgaa
    41521 attgaccgcg tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta
    41581 ccgcctgccg ttgatcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc
    41641 caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg
    41701 attctggatg ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt
    41761 ttgattgctg gaggcgaata tgccattcag cacgcggtgg aaggagctga agatagccgg
    41821 gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc
    41881 attgctgcca gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc
    41941 ggctgccgca cagtgggaat ttcctgtaat ccggggagcg ccgtttcaac caccgctgag
    42001 tttgccatta caccgattgt aggtgccgaa gttgttaccg gttcttcgcg gatgaaagca
    42061 ggtacagcgc agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatccggc
    42121 aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc
    42181 caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta
    42241 attgcctgcg agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc
    42301 gcagaagcta aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag
    42361 gaataaccca tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc
    42421 ggcggaccgg gaaatatcgc cagttgtggt aactgtatga cgcgcctgcg tctgggtgta
    42481 catgacagtt cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt
    42541 ttgaccagcg accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa
    42601 gcgatgagcg agctgctggg cgatgcaccg gtacaggatg ccgcagaaat cgccgcccag
    42661 aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc
    42721 gccaccatct tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata
    42781 gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac
    42841 gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca ccttcctagt gattctggtg
    42901 ggctataacg ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca
    42961 ctctttttgc tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat
    43021 ttctttggtc tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg
    43081 gcctgcgccc gcattgaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg
    43141 ctgacctcgt taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg
    43201 ctgggtggct ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg
    43261 ttcggttgtg cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag
    43321 ggctttattc ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc
    43381 atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg
    43441 caaccgcaca gtgcgctacg cagtcaggta cgcggggcga ttattcccgg cctgctgggc
    43501 gttggtgaac cgctgattta cggtgtcacc ctgccccgca tgaagccgtt tgttaccgcc
    43561 tgtttaggcg gcgcggcggg cggtttgttt atcggattga tagcctggtg gggtctgccg
    43621 atgggcttaa acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc
    43681 gcacaaggca tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt
    43741 tgcgggttta ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatattat
    43801 gaaacggaca atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa
    43861 ataccctgtt ctgacagaag gtagttgatc aagtatatgc ggcgttaaag cagtaataaa
    43921 aaggccagtc agtaatgtta ctgcctggcc tttaatcaat gaatcagaaa cgcttacagc
    43981 gccatcaact tgtccagcga cggtgcgaaa taatagccgc cggttaccgg tttggtgaaa
    44041 cgcaacatcg catcacgttt gccatcggta tcgccaaaca tgctcagcag ttgctgctca
    44101 atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc actggcggtg
    44161 ccgtacggca ggctctggcg aacaatcttc agccctttgc catcttcttt cagatcaacg
    44221 cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg
    44281 gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc ggttgagctg cttcaggttg
    44341 tgctcccaac gctgcacaaa cacatagctg ccgccggaat ccacgccgtc tttgataacc
    44401 gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac aaagccgctc
    44461 aggtcacgct cttccaccca acggaagccg tggatctctt ctttcacttc aatgcagtca
    44521 ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta cgtcgtgacg cagagaaaga
    44581 atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc ctttaccgta gcccggaaaa
    44641 tctttcagct cttctgcccc aacgccgccg ctcagagcgc gccaggtgtt gttaccaaag
    44701 gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg
    44761 gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc
    44821 caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga cattgttcct
    44881 cctgaaaata agaatgccac gcaatccgtg gcattgttga gcgctatttt acgtgttttt
    44941 tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca gataatttta ctgactttcc
    45001 agtttttcag ggtatcgtcc gaaggcatta acccttcagg accacgccat tccccgctga
    45061 actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga ttatcatcac
    45121 catcagcttt ttggcaattg ccaaatgctt tgctgtaaag gtcgctaaac ggtgtaccga
    45181 ttttaacacc ggtgtcagca ggaatatcgc tatccagcac gtcaatgcga ctgatcgtgc
    45241 cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt catcacttca aaaaagcgca
    45301 ccacgttgcc gttcgtggtt ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat
    45361 cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc
    45421 tcactttggt ggacgatcca aaccagttcc acgggttagc ggcagaccag ttaactgacg
    45481 acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa cgcagcgatt
    45541 tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc accaaaaagt
    45601 gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga
    45661 ttaacgccac aatatccgcc actaccagcg ccagaccgat accgctaacg gattcaccgt
    45721 tcagccacag ccacggttgc cagcaaagta aaaccacctg cgccaacagc aacagaaaat
    45781 agagcacatg ccaggtgcga ggaaacgaag cccgccgacc gctcaacaaa aacgccagca
    45841 ccgccggaat accgggaatc agccccagcc agaaattatc gtgatcggga taaaacagat
    45901 ttagcagcgc agtaccctgc tcacgcgacg caccggcaat gacaaacagc acccaggttc
    45961 gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcgt ccgtgcgcat
    46021 cataatggac aggatggaac tcagtactct tcatcttcaa tcaaacgctt acccagactc
    46081 agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat gtcgttatct
    46141 tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa tcagcttttt ctccagccgg
    46201 ttaagcaacg cattggcaat tccacgccca cgaaactctg gatgcacgcc aagataataa
    46261 gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc accgtttacc
    46321 tcagcgacca aaaacaaact gacgtcatgg ttcatcttac gctcgatgtc catttccgga
    46381 tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct
    46441 tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt taaaaggctg
    46501 attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga aaaagtggca
    46561 taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc
    46621 gtaacggatt acgcgatacg atataacatc tgaaacttta ttattacaac tcaggccgta
    46681 tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg aaagccggtt
    46741 tagctgccct gacgttgtca ggaatgtcgc aagccattgc caaagacgaa cctttaaaaa
    46801 ccagcaacgg acacagcaag ccgaaagcca aaaaatctgg cggcaaacgt gtcgttgttc
    46861 tcgatccagg ccacggcgga attgataccg gagcgatcgg acgcaacggt tcgaaagaaa
    46921 aacatgtggt gctggcgatt gctaaaaacg tccgttccat tttgcgtaat catgggattg
    46981 atgcgcgttt aacgcgttct ggcgatacgt ttatcccact ttacgatcgc gttgaaatcg
    47041 cccataaaca tggcgcagat ctgtttatgt caattcatgc cgatggcttt accaacccga
    47101 aagctgccgg tgcttcggta tttgccctct ctaaccgtgg ggcaagtagc gcaatggcga
    47161 aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaag gcgactgaca
    47221 aggatcacct attgcagcaa gtactgtttg atctggtgca aaccgatact attaaaaaca
    47281 gcctgacgct tggctcgcat attctgaaaa agattaagcc ggtgcataaa ctgcacagcc
    47341 gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttcct tcggtgctgg
    47401 tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg gcggcgtttc
    47461 gtcagaaaat cgccacagcg attgctgaag gcgtgatcag ttatttccac tggttcgaca
    47521 accagaaagc acattcgaaa aagcgataag ttatgaaacc cgacgcacac caggttaaac
    47581 agtttctgct caaccttcag gatacgattt gtcagcagct gaccgccgtc gatggcgcag
    47641 aatttgtcga agatagttgg cagcgcgaag ctggcggcgg cgggcgcagt cgggtgttgc
    47701 gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc tcacgtccac ggtgaggcga
    47761 tgcctgcttc cgccaccgct catcgcccgg aacttgccgg gcgcagtttc gaggcgatgg
    47821 gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc caccagccac gcgaatgtgc
    47881 gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg gtggtttggc ggcggcttcg
    47941 atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc accgcccgtg
    48001 acctgtgcca gccatttggt gaagacgttt atccccgtta caaaaagtgg tgcgacgatt
    48061 acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc tttgatgatc
    48121 tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc aaaggctaca
    48181 ccgacgctta tttaccaatt gtagagcgac gtaaagcgat ggcctacggc gagcgcgagc
    48241 gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg
    48301 gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg tcaatgccgc
    48361 cactggtacg ctgggagtat gattatcagc caaaagatgg cagcccagaa gcggcgttaa
    48421 gtgagtttat taaggtcagg gattgggtgt aactccctca cccccattcc cgcatccgct
    48481 gatgcaacgt caacgacggc ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc
    48541 ccagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg
    48601 ggcttatcag ttcgcggcgt acgacgttca ggcgaatgcg tttcagccac gcgtttgggc
    48661 caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg gcgactgaca tgcagttgat
    48721 tacacaaatc cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc
    48781 gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc attggttgcg
    48841 cttcttccag catcgccccc atcgccatta gcaaattatc ccccagcact tttcgcactg
    48901 caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc
    48961 cccacagcgc ggctttatgc tgctctttca cttccagcgc cgactggcta cgcagcatat
    49021 gtaatacccg atccggattg tgcaaaaagt tagcctgaca ggtgatgaca tcttcagaca
    49081 gcactacgcc caggatcgta taatcgtccg gcgtacttaa ttcaaactcg gttccaccgg
    49141 ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc gataaatccc tgctcaccgc
    49201 gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac tgacgcagcg
    49261 ccagaccggt gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac
    49321 catgaaactt gcccggatgc agctgatcgt aaatctgctg ctaggcggta atcgtcaaag
    49381 catgttcata gacatccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg
    49441 tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg
    49501 tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat
    49561 ggggcttgag acgataacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt
    49621 cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcggccact tctcctgccg
    49681 tcattccctg acatactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga
    49741 taactggcgc gtcgggttcc tttacgggtt gcttcggctg gcggttaaag ccagtaacca
    49801 gccactgggt gtcatcgtct ggccgaccga tctccttacg gctgatgaca cgcccggtac
    49861 gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca
    49921 ccaccagcgt taagcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg
    49981 ctttgagcat ggcatccgct gcatctatcg cggcgaccat gccgtccact tccagcaatc
    50041 ccagtgcatt gatcatcggc aacctccgtt acgcacgctg gattggatta cgcgcgattt
    50101 ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg ctaccggtta
    50161 aaaatgctgc cgagtagttg gtttcagacg gtggcgggac gtaggtcacc agctgtacgt
    50221 cggcagattt caacgcggca tcaatgccgt aggtcgcttc cagcggcggt gccaccagat
    50281 acgccatcgg atcgccaagc gtgatcccgg cggttgatga aagataagaa ccggtacgcg
    50341 aaaccacatg cgccaggaag gcggtgtttt ctgcgtcatt ggcccactgg aaagctgcgc
    50401 cattttcaat atgtgcaacc atcgcatcca gaccggcacg cacttccgcc gggtttggcc
    50461 caccaagcat aatcagcact tcaccggcag tcggtgacgg gccgtgtgcc gctcccgcat
    50521 acagcgagcg accatatacc acttcgacca tcgcttgttt ggtcgcttcg tcagccgcaa
    50581 tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag gctacgaata tgcggcggca
    50641 attttagttc acgcgcaaat tcagcgttaa cggaggcaat cacccgcatg gcggttaccg
    50701 acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgccgg
    50761 atgctttctg ctccagcata cgtttcgcca aatccacaat gacggcacag cagcacgaaa
    50821 gagaagcaaa agtcagctac ctggtacact aggcagcggg tcagcaaagg ggggcaaatg
    50881 cccctttttt catgtcagat aatcagatca ttacctcaat aaactaataa tcacatcgcg
    50941 gttacaacca gtttgatagt gatagctcaa acccagacgt ggcgctggct gcaagcaaaa
    51001 taagccgcaa aaatgcgacc ccatgtttac tagccgcaaa aatgcggtcc tatttgccag
    51061 aaccttttat tattcaattg gttagtgcaa gaaaaagcgt gattactgtt caagaagtat
    51121 ccagaaaggt catgattttg acgctgaaaa aaaataggtg ggcatttttg cccactcact
    51181 agccgcaaat ttgcccactc actagccgca tttttgcggc ccaaagtaat aaatacagag
    51241 aaataataaa tacagatcag cgccaagatt tttcgtcttt tcggcggaat catgagacta
    51301 gtgcttacgt cctgataacc tccagagcag accttaactt ctgtgcagca ggactacgct
    51361 cattgccgct cggctaggtc aaatcctcac gataaagctg ttcggattgt gaccataatg
    51421 ttaaaggact ctttaaatca ttccttgacg aattaactag ctggcttaag cggaatagca
    51481 tgcaggataa aaacaaaacc agcttagcag tggggagagc ttcgcttcgc tcgtctcctg
    51541 gggccaatgc tcaatgaaac gacgtaaggc acggagcgct caccataggt agacacataa
    51601 acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat aaaaaccaac gacaccgcca
    51661 cactgcaaga ccaatggttt ttatcgcgta tacagaccag acaggaaaca gcagcaaagc
    51721 agcgtatagc caggtgcaga cagcacactc acacgacgca cagcaggctt aagtgatcac
    51781 tcaggaaact ttatccccgc cgccgatcac cgcgaactat catgaccgcg ccaaattcga
    51841 aaggaatggc ccatagaacg cgatgaacac acataagcgc tacacttact taacgtcgcc
    51901 acagagcgat tgagagcgct taaaatcgat gcgatttcat taaatttttc tcactcgcct
    51961 gaatggtggc gatcaggact gatcgcaaca ttgccccgcc tcgatattga gcgcataata
    52021 gatacataca ttaagcggaa aagagataag tttattgtga aaatcagggg gttacatatc
    52081 atgcgcatat cagaacaaca acggcagatc ctcgactatc tggcaaaaaa cgaatcaatc
    52141 accgcacgac aagccgcaga tctcgtttat ggtggcaatg ttacccgcgt ccaggttgac
    52201 gccgcccgcc gctcactcat gacgatggtt agtaatgggc taatcaggaa acaaggccgc
    52261 gctttcgtag ctgaaagaca tatcaatcac gcttatagcc aggacgttga agaattgcgc
    52321 cgccagatcc tttgggtatt cagcaaacag ggtcaggagt atttcctttc tgaacaccca
    52381 aatttcgccg ccaaagagtt aacagtaagc ggattgtcgt gcgcactgga ttatcttgtc
    52441 ggtgaaggtt gcgttactta caagcagcta tggcacgccc tgaatacgct tgtagaagat
    52501 ggtttactca ttgccacgcg tgagaacatc gaatactgga taatgccgcg ttcaggtcgc
    52561 cgcaatcatg cacggtatat ttcaggcaac agatacagaa cgccggactt taatccgaca
    52621 caggaagatc gggaccgtgc cgaaatgatg tgcagggaac aggagaaaag aattatggat
    52681 aagttatttt cgatgcagtg aacacaagcg ggcgcaatgc ccgtttttat tgccttattc
    52741 cacgcctgac gaatcaattt gatcacacct gagaaaatgc atagccagaa gttaaggccg
    52801 ttatattgcg attgggagcg ttttaaacgg tatcaattta attaattctg atgtggtgat
    52861 ttgcaggcaa aaaaaacccg ccggagaagc gggaaataga gacatatcgt tgtagttatt
    52921 aacagatcac tttgaggtcg agggcggcta accctcgcta atattatccc gctattacct
    52981 tgcgggtgca aataatcttt tattgctttg atgatttaat aaatagcact ttttgctaac
    53041 aaaaaccgct gtcaatatat ggccaaaaat gtcaacagaa aaaataaggt ttataatatt
    53101 aagtatcaca tgggtaatgg gttcagggaa tcggaatcca ccgcctcacg gaaattcaaa
    53161 atttgcttct tctttcctcc gtgggcggct tctttttcgt atcgaggtat ttatgaaagc
    53221 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    53281 gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta ctcgccaatt
    53341 attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat aatcacgcat
    53401 cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt gccgatatta
    53461 tgcgacgcag atggatttat gaccctgaag cgggtttatt gtttagccgt gaaacaaaag
    53521 acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta catgaaggta
    53581 aattcaaagt gacactcacc tatcagaaag cctgctatgt atacacatac ggcgcttatg
    53641 acgagacatt atatgaagtg caccacgtta atttgaacaa acaggataac cgcatcaaga
    53701 atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc cgcaaactta
    53761 ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca ctataaacac
    53821 aggagtaaat accatgcatt cattagacag ttattttcag cgcacgacag cccctaaatc
    53881 agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg ctgattatat
    53941 tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg ataaacttca
    54001 gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt gcgaattaag
    54061 tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg ggaaagataa
    54121 tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag cgtgataaaa
    54181 ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc cgtatggccc
    54241 agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc ccacaaatcg
    54301 ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta aaaacgaacg
    54361 cagagaaaat ggagatgtac aaacagagcg gtgctccgaa tcccgatgag tacttctcac
    54421 cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc accgaggaac
    54481 agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac tcgtatttcg
    54541 gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg
    54601 ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg attcggtact
    54661 gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga ccaatgaaca
    54721 aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac tatattagca
    54781 caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc gctaagaccc
    54841 tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc atgaagcgcc
    54901 aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc atcgacatga
    54961 aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt ttaattctca
    55021 ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat gatgggacgg
    55081 gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga agcaagatat
    55141 tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca
    55201 ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca tcagtttcac
    55261 caattactgc atctacgagt gttacaagga agatgcagag gactggctgg attcattgcc
    55321 agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc atatcacctc
    55381 tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc acaataacga
    55441 tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat tgaaacacat
    55501 ctaccatgac cataataaag taatttttt
//
LOCUS       DAZPFP010000008        55420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPFP010000008 DAZPFP010000000
VERSION     DAZPFP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.008"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUR4_000432"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUR4_000432"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1454165.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUR4_000433"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUR4_000433"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1454166.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUR4_000434"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUR4_000434"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1454167.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHUR4_000435"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHUR4_000435"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1454168.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUR4_000436"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUR4_000436"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1454169.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHUR4_000437"
     CDS             7826..9559
                     /locus_tag="ACHUR4_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1454170.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHUR4_000438"
     CDS             9708..10559
                     /locus_tag="ACHUR4_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1454171.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHUR4_000439"
     CDS             complement(10546..10887)
                     /locus_tag="ACHUR4_000439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1454172.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHUR4_000440"
     CDS             complement(10889..11767)
                     /locus_tag="ACHUR4_000440"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1454173.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHUR4_000441"
     CDS             complement(11733..14030)
                     /locus_tag="ACHUR4_000441"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1454174.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHUR4_000442"
     CDS             complement(14081..14401)
                     /locus_tag="ACHUR4_000442"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1454175.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHUR4_000443"
     CDS             complement(14416..15495)
                     /locus_tag="ACHUR4_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1454176.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000444"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUR4_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUR4_000445"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUR4_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUR4_000446"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUR4_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHUR4_000447"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHUR4_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUR4_000448"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUR4_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1454177.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUR4_000449"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUR4_000449"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1454178.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUR4_000450"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUR4_000450"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1454179.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHUR4_000451"
     CDS             25474..25608
                     /locus_tag="ACHUR4_000451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454180.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUR4_000452"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUR4_000452"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1454181.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUR4_000453"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUR4_000453"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1454182.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUR4_000454"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUR4_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1454183.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHUR4_000455"
     CDS             30120..30728
                     /locus_tag="ACHUR4_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1454184.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUR4_000456"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUR4_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1454185.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHUR4_000457"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHUR4_000457"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1454186.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUR4_000458"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUR4_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1454187.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUR4_000459"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUR4_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1454188.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUR4_000460"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUR4_000460"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1454189.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUR4_000461"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUR4_000461"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1454190.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHUR4_000462"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHUR4_000462"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1454191.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUR4_000463"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUR4_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1454192.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUR4_000464"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUR4_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1454193.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUR4_000465"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUR4_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUR4_000466"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUR4_000466"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1454194.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUR4_000467"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUR4_000467"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1454195.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUR4_000468"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUR4_000468"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1454196.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUR4_000469"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUR4_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1454197.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHUR4_000470"
     CDS             complement(44843..45142)
                     /locus_tag="ACHUR4_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1454198.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHUR4_000471"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHUR4_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHUR4_000472"
     CDS             45895..46494
                     /locus_tag="ACHUR4_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1454199.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUR4_000473"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUR4_000473"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1454200.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUR4_000474"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUR4_000474"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1454201.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUR4_000475"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUR4_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1454202.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUR4_000476"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUR4_000476"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1454203.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUR4_000477"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUR4_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1454204.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUR4_000478"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUR4_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1454205.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUR4_000479"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUR4_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1454206.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUR4_000480"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUR4_000480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1454207.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUR4_000481"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUR4_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1454208.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15017 c        14165 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAZPFP010000009        54463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPFP010000009 DAZPFP010000000
VERSION     DAZPFP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.009"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(67..1011)
                     /gene="leuO"
                     /locus_tag="ACHUR4_000482"
     CDS             complement(67..1011)
                     /gene="leuO"
                     /locus_tag="ACHUR4_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1454209.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            1670..1756
                     /gene="leuL"
                     /locus_tag="ACHUR4_000483"
     CDS             1670..1756
                     /gene="leuL"
                     /locus_tag="ACHUR4_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1454210.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            1849..3420
                     /gene="leuA"
                     /locus_tag="ACHUR4_000484"
     CDS             1849..3420
                     /gene="leuA"
                     /locus_tag="ACHUR4_000484"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1454211.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            3420..4511
                     /gene="leuB"
                     /locus_tag="ACHUR4_000485"
     CDS             3420..4511
                     /gene="leuB"
                     /locus_tag="ACHUR4_000485"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1454212.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            4514..5914
                     /gene="leuC"
                     /locus_tag="ACHUR4_000486"
     CDS             4514..5914
                     /gene="leuC"
                     /locus_tag="ACHUR4_000486"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1454213.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            5925..6530
                     /gene="leuD"
                     /locus_tag="ACHUR4_000487"
     CDS             5925..6530
                     /gene="leuD"
                     /locus_tag="ACHUR4_000487"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1454214.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(6579..7757)
                     /gene="setA"
                     /locus_tag="ACHUR4_000488"
     CDS             complement(6579..7757)
                     /gene="setA"
                     /locus_tag="ACHUR4_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1454215.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(7859..7990)
                     /gene="sgrT"
                     /locus_tag="ACHUR4_000489"
     CDS             complement(7859..7990)
                     /gene="sgrT"
                     /locus_tag="ACHUR4_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1454216.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            8079..9734
                     /gene="sgrR"
                     /locus_tag="ACHUR4_000490"
     CDS             8079..9734
                     /gene="sgrR"
                     /locus_tag="ACHUR4_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1454217.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      9768..9867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9898..10881
                     /gene="thiB"
                     /locus_tag="ACHUR4_000491"
     CDS             9898..10881
                     /gene="thiB"
                     /locus_tag="ACHUR4_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1454218.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            10857..12467
                     /gene="thiP"
                     /locus_tag="ACHUR4_000492"
     CDS             10857..12467
                     /gene="thiP"
                     /locus_tag="ACHUR4_000492"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1454219.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            12451..13149
                     /gene="thiQ"
                     /locus_tag="ACHUR4_000493"
     CDS             12451..13149
                     /gene="thiQ"
                     /locus_tag="ACHUR4_000493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1454220.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(13263..14027)
                     /locus_tag="ACHUR4_000494"
     CDS             complement(13263..14027)
                     /locus_tag="ACHUR4_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1454221.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(14113..14991)
                     /gene="araC"
                     /locus_tag="ACHUR4_000495"
     CDS             complement(14113..14991)
                     /gene="araC"
                     /locus_tag="ACHUR4_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1454222.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            15330..17030
                     /gene="araB"
                     /locus_tag="ACHUR4_000496"
     CDS             15330..17030
                     /gene="araB"
                     /locus_tag="ACHUR4_000496"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1454223.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            17041..18543
                     /gene="araA"
                     /locus_tag="ACHUR4_000497"
     CDS             17041..18543
                     /gene="araA"
                     /locus_tag="ACHUR4_000497"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1454224.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            18743..19438
                     /gene="araD"
                     /locus_tag="ACHUR4_000498"
     CDS             18743..19438
                     /gene="araD"
                     /locus_tag="ACHUR4_000498"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1454225.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            19513..21864
                     /gene="polB"
                     /locus_tag="ACHUR4_000499"
     CDS             19513..21864
                     /gene="polB"
                     /locus_tag="ACHUR4_000499"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1454226.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            22029..24935
                     /gene="rapA"
                     /locus_tag="ACHUR4_000500"
     CDS             22029..24935
                     /gene="rapA"
                     /locus_tag="ACHUR4_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1454227.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            24947..25606
                     /gene="rluA"
                     /locus_tag="ACHUR4_000501"
     CDS             24947..25606
                     /gene="rluA"
                     /locus_tag="ACHUR4_000501"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1454228.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(25723..26538)
                     /gene="djlA"
                     /locus_tag="ACHUR4_000502"
     CDS             complement(25723..26538)
                     /gene="djlA"
                     /locus_tag="ACHUR4_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1454229.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26793..29147
                     /gene="lptD"
                     /locus_tag="ACHUR4_000503"
     CDS             26793..29147
                     /gene="lptD"
                     /locus_tag="ACHUR4_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1454230.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29200..30486
                     /gene="surA"
                     /locus_tag="ACHUR4_000504"
     CDS             29200..30486
                     /gene="surA"
                     /locus_tag="ACHUR4_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1454231.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30486..31475
                     /gene="pdxA"
                     /locus_tag="ACHUR4_000505"
     CDS             30486..31475
                     /gene="pdxA"
                     /locus_tag="ACHUR4_000505"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1454232.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31472..32293
                     /gene="rsmA"
                     /locus_tag="ACHUR4_000506"
     CDS             31472..32293
                     /gene="rsmA"
                     /locus_tag="ACHUR4_000506"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1454233.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32296..32673
                     /gene="apaG"
                     /locus_tag="ACHUR4_000507"
     CDS             32296..32673
                     /gene="apaG"
                     /locus_tag="ACHUR4_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1454234.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32680..33522
                     /gene="apaH"
                     /locus_tag="ACHUR4_000508"
     CDS             32680..33522
                     /gene="apaH"
                     /locus_tag="ACHUR4_000508"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1454235.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(33600..34079)
                     /gene="folA"
                     /locus_tag="ACHUR4_000509"
     CDS             complement(33600..34079)
                     /gene="folA"
                     /locus_tag="ACHUR4_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1454236.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34270..36132)
                     /gene="kefC"
                     /locus_tag="ACHUR4_000510"
     CDS             complement(34270..36132)
                     /gene="kefC"
                     /locus_tag="ACHUR4_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1454237.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36125..36655)
                     /gene="kefF"
                     /locus_tag="ACHUR4_000511"
     CDS             complement(36125..36655)
                     /gene="kefF"
                     /locus_tag="ACHUR4_000511"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1454238.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36703..38097)
                     /locus_tag="ACHUR4_000512"
     CDS             complement(36703..38097)
                     /locus_tag="ACHUR4_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454239.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(38156..38443)
                     /gene="fixX"
                     /locus_tag="ACHUR4_000513"
     CDS             complement(38156..38443)
                     /gene="fixX"
                     /locus_tag="ACHUR4_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1454240.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38440..39726)
                     /locus_tag="ACHUR4_000514"
     CDS             complement(38440..39726)
                     /locus_tag="ACHUR4_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1454241.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39777..40718)
                     /locus_tag="ACHUR4_000515"
                     /pseudo
     CDS             complement(39777..40718)
                     /locus_tag="ACHUR4_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(40733..41503)
                     /locus_tag="ACHUR4_000516"
     CDS             complement(40733..41503)
                     /locus_tag="ACHUR4_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1454242.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41859..43373
                     /gene="caiT"
                     /locus_tag="ACHUR4_000517"
     CDS             41859..43373
                     /gene="caiT"
                     /locus_tag="ACHUR4_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1454243.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43404..44546
                     /gene="caiA"
                     /locus_tag="ACHUR4_000518"
     CDS             43404..44546
                     /gene="caiA"
                     /locus_tag="ACHUR4_000518"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1454244.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44675..45892
                     /gene="caiB"
                     /locus_tag="ACHUR4_000519"
     CDS             44675..45892
                     /gene="caiB"
                     /locus_tag="ACHUR4_000519"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR1454245.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            45966..47519
                     /gene="caiC"
                     /locus_tag="ACHUR4_000520"
     CDS             45966..47519
                     /gene="caiC"
                     /locus_tag="ACHUR4_000520"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1454246.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47628..48413
                     /gene="caiD"
                     /locus_tag="ACHUR4_000521"
     CDS             47628..48413
                     /gene="caiD"
                     /locus_tag="ACHUR4_000521"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1454247.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48419..49009
                     /gene="caiE"
                     /locus_tag="ACHUR4_000522"
     CDS             48419..49009
                     /gene="caiE"
                     /locus_tag="ACHUR4_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1454248.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49095..49490)
                     /gene="caiF"
                     /locus_tag="ACHUR4_000523"
     CDS             complement(49095..49490)
                     /gene="caiF"
                     /locus_tag="ACHUR4_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1454249.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49752..52973)
                     /gene="carB"
                     /locus_tag="ACHUR4_000524"
     CDS             complement(49752..52973)
                     /gene="carB"
                     /locus_tag="ACHUR4_000524"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1454250.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(52991..54139)
                     /gene="carA"
                     /locus_tag="ACHUR4_000525"
     CDS             complement(52991..54139)
                     /gene="carA"
                     /locus_tag="ACHUR4_000525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1454251.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        13179 a        14739 c        14187 g        12358 t
ORIGIN      
        1 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc
       61 attcagttag cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc
      121 gcgcccggca gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag
      181 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
      241 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
      301 cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg
      361 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt
      421 aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
      481 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg
      541 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
      601 aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac
      661 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
      721 ttgtactagt tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg
      781 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
      841 aacagcgtta ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt
      901 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
      961 tggcttgctt aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc
     1021 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata
     1081 tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa
     1141 gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat
     1201 aataatccat aatgttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat
     1261 catctacaaa atggattaaa tgtgcttatt taataattaa tttatatatt taatgcatta
     1321 attcttaaca ttaattgttc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa
     1381 catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt
     1441 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
     1501 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
     1561 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
     1621 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
     1681 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
     1741 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
     1801 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
     1861 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
     1921 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
     1981 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
     2041 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
     2101 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
     2161 atgcacatcg ccaccaagct gcgcagcacc ctagacgagg tgatcgaacg cgctatctat
     2221 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
     2281 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
     2341 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     2401 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     2461 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     2521 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     2581 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccaa
     2641 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     2701 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     2761 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     2821 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     2881 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     2941 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     3001 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     3061 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     3121 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     3181 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     3241 gatattgtca ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     3301 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     3361 gtcgaaaaag agttgcaacg caaaactcaa cacaacgaaa acaacaagga aaccgtgtga
     3421 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     3481 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     3541 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     3601 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     3661 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     3721 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     3781 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat ccagtgcgtg cgcgaactga
     3841 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     3901 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     3961 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     4021 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     4081 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     4141 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     4201 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     4261 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     4321 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     4381 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     4441 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     4501 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     4561 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     4621 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     4681 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     4741 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     4801 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     4861 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     4921 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     4981 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     5041 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     5101 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     5161 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccgctctgg ttgctcctga
     5221 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     5281 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     5341 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     5401 ggtgatttct gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     5461 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     5521 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tctcgcattg aagatttacg
     5581 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     5641 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     5701 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     5761 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     5821 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     5881 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     5941 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     6001 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     6061 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     6121 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     6181 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     6241 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
     6301 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
     6361 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
     6421 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
     6481 acgacgctat tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc
     6541 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
     6601 gttaaaaata atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc
     6661 caactttgtg caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg
     6721 ttggtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
     6781 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
     6841 gccatacggc tatgaaagaa aatcaatcca gtgtaaaaca gcactcctgc caccactgca
     6901 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
     6961 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
     7021 aaatcgctac tgatccacaa tggcatatca ataatgtaca tagtgttgca ggtccacatt
     7081 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagtcacc ttgcattgat
     7141 aaagcatttt ccgacggcag ttctacccgc gccacagaca gaagcataaa tgcaatcaat
     7201 accagactga gtgtgaatat cccggcggca atcgaaaaca tcactgtaaa gccgtaattc
     7261 aacgccagca taaaggccag cggtggaccg attacccatg ccagagaaag ctgcgcacgc
     7321 atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcgtattc ccgcgccaga
     7381 gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata
     7441 agcgtcagat aatgacgatt aaatgcaaac agtagcgcat tgccaatagc catcaaacag
     7501 caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg
     7561 cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc
     7621 gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc
     7681 cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc
     7741 gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg
     7801 tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc
     7861 aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg
     7921 ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa
     7981 actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa
     8041 aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac
     8101 aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg
     8161 aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc
     8221 aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga
     8281 cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg
     8341 atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg
     8401 tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc
     8461 gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc
     8521 ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca
     8581 tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag
     8641 tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa
     8701 tcaatacgct gccgctctat tcgcatatta cggacattgt gtcgccgacg ccctggacgc
     8761 tggatatcca tctcacgcag ccggaccgct ggttgccgtt actgctgggg caagttccgg
     8821 cgatgatcct gccgtgcgaa tgggaaaccc tcagtaactt tgccagccat cctattggca
     8881 ccggtccgta tgcggtgatt cgcaacagca ccaatcaact gaaaattcag gcattcgatg
     8941 acttcttcgg ttaccgggca ttaatcgacg aagttaacgt ctgggttctg ccggaaattg
     9001 ccgacgagcc agccggtggt ctgatgctaa aaggtccaca gggcgagaaa aaagagattg
     9061 aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg
     9121 cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt
     9181 tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc
     9241 accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct
     9301 tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa
     9361 gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga
     9421 tcgagagcga tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt
     9481 tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg
     9541 acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg
     9601 ccagtaaagc aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta
     9661 tgcgcggcct gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc
     9721 caccggatcc atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg
     9781 ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg
     9841 gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg
     9901 ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt
     9961 ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa
    10021 aaagcttttg aagccaactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt
    10081 tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg
    10141 ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg
    10201 gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat
    10261 tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg
    10321 aaagaattgg ttgagagcga tcagaactgg cgggtgattt atcaggatcc gcgtaccagc
    10381 acgccggggc tgggtctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa
    10441 gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac
    10501 ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat
    10561 cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat
    10621 ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa
    10681 ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg
    10741 tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc
    10801 acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg
    10861 caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc
    10921 tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta
    10981 actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc
    11041 aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct
    11101 atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc
    11161 tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg
    11221 catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggca
    11281 ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac
    11341 tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc
    11401 attttttccg cttcgtcgaa tggccgtggc tacggcgaca aatcccgccg gttgccgcgc
    11461 ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc
    11521 aggcaaccac tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc
    11581 gcgcggcgat gctggcgctg ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc
    11641 agcgactaag taaggccatt gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg
    11701 acgatcgtct gcatagccgc atttgcgaca cggtgttaat agtgctggcg ctgctgctgt
    11761 tgctgccacc gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc
    11821 tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag
    11881 gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc
    11941 ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc
    12001 cggggattgt gctggctacc ggcttctttt tactgctcaa caacactatc ggcctgccac
    12061 aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga
    12121 aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc
    12181 tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac
    12241 tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt
    12301 tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct
    12361 atcgcagcca ggacggcgcg gtcaccgcgt taattctgct actactctgt tttctgctgt
    12421 ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt
    12481 tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc
    12541 ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgattgccgg ttttctgacg
    12601 ccagccagcg gttcgctgac tatcgatggt gttgatcaca ccacgacgcc gccgtcacgc
    12661 cgtccggtgt cgatgctgtt tcaggagaac aacctgttca gccatctgac ggtcgcgcag
    12721 aacatcgggc tggggctaaa tccggggttg aaactgaacg cggcacagca ggagaaaatg
    12781 cacgcgattg cccgtcagat ggggattgat aatttaatgg cgcggttacc gggcgagctt
    12841 tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt
    12901 ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga gatgctgacg
    12961 ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg
    13021 gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg
    13081 cagggtaaga ctgaagaatt gttgagcggt aaggcgagtg cttcggcaat attggggatt
    13141 acgggttagt atgcactgtc agatgcggcg tgaacacctt atccgaccta cggacagtgc
    13201 gttttgtagg cctgataaga cgcggtcagc gtcgcatgag gcaacccgca caagacaatc
    13261 ccctaaaccc caaccacttt acgcaaaata tcgatataca ccggcatcaa cgggtggcga
    13321 attaacacca ccagcgccac cacgccgatg gcagaaatca acggcgtcag ccacaacaaa
    13381 cggccgcgcg acaaataatg acttaaacga tcggttgctt taccgctgcg ccataaccgc
    13441 cagcacagcc agccaccaac ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac
    13501 tcgccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat cccaggcagg
    13561 aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa ttttgccacc
    13621 ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaagcga
    13681 ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat
    13741 gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg ccagcccagc
    13801 cagaaggaga tccagtcgcc cagcagacaa ccgacaatcc ctgccagcca agcgtgccag
    13861 aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt
    13921 agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat
    13981 gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca
    14041 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
    14101 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
    14161 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
    14221 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
    14281 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
    14341 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
    14401 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg
    14461 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
    14521 ctcaagcaga ttcatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
    14581 gatttgccca aacaggtcgc tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc
    14641 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
    14701 gtaaacccac tggtgatacc attcgcgagc ctccggatga caaccgtagt gatgaatctc
    14761 tcctggcggg aacagcaaaa tatcacccgg tcggcagaca aattctcgtc cctgattttt
    14821 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
    14881 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
    14941 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
    15001 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
    15061 cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta aaagcattct
    15121 gtaacaaagc aggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
    15181 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
    15241 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
    15301 atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc gattttggca
    15361 gtgattctgt gcgagctttg gcggtggact gcgccagcgg tgaagagatc gccaccagcg
    15421 tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat aaccagttcc
    15481 gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc gtgcttgcag
    15541 agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacagt accggctcga
    15601 cgcccgcacc gattgatgcc gacggtaacg tgctggcgct gcgcccggag tttgccgaaa
    15661 acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa gcggaagaga
    15721 ttacccgttt gtgccacgcg ccgggcaacg ttgactactc ccgctatatt ggcggtattt
    15781 attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg
    15841 cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta
    15901 ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc
    15961 acgaaagctg gggcggcttg ccgccagcca gtttctttga tgagctggac ccgatcctca
    16021 atcgccattt gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca
    16081 ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg
    16141 gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa
    16201 aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc
    16261 gggcagttaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc
    16321 tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct
    16381 ggccgctgga acagcttgcc gcccagcatc cggaactgaa agcgcaaatc aacgccagcc
    16441 agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc
    16501 tgccggtggt gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac caacgcctga
    16561 aaggggtgat taccgatctt aacctcgcta ccgacactcc gctgctgttc ggcggtttga
    16621 ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg
    16681 ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg
    16741 cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc
    16801 tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc
    16861 agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac
    16921 gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc
    16981 ttccaacttc cgccccggca caggctgccc aggccgttgc gactctataa ggacacgata
    17041 atggcgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc
    17101 ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc gctgaatacg
    17161 gaagcgaaac tgccctacaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc
    17221 accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg
    17281 cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg
    17341 ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagtatcga tatggacttt
    17401 atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg cgcgcgtatg
    17461 cgtcagcaac atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc
    17521 ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga
    17581 tttggcgata acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc
    17641 aagttcggtt tctccgtcaa tacctgggcg gttggctatc tggtgcaggt ggtgaactcc
    17701 atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg
    17761 cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag
    17821 ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa
    17881 gatttgcacg gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat gcagcagggt
    17941 tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat catgaaggtg
    18001 atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag
    18061 aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc gattgccgta
    18121 gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc
    18181 cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat tgatctcggc
    18241 gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca ctccctgccg
    18301 aaactgccgg tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac tgcttccgaa
    18361 gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac
    18421 gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc
    18481 cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttcgtcgc
    18541 taagtagcca catcaggtat gtaacgcctg atgcgacgct gacgcgtctt atctggccta
    18601 cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg
    18661 acgctggcgc gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcgaagcg
    18721 catccggcac gaaggagtca acatgttaaa agatctcaaa cgccaggtat tagaagccaa
    18781 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    18841 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    18901 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    18961 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    19021 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc
    19081 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    19141 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    19201 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    19261 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    19321 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    19381 gctgctgcat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    19441 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    19501 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    19561 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    19621 gcaccgcaag agtccgtggc gtttattcac gccgatcagg ttccccgcgc tcagcatatt
    19681 ttgcagggtg aacaaggctt tcgtctgaca ccgctggcgt taaaggattt tcaccgccag
    19741 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgtctg
    19801 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    19861 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    19921 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    19981 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    20041 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    20101 tacgtcgcca gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat
    20161 cccgatgtga tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    20221 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    20281 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    20341 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    20401 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    20461 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    20521 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    20581 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    20641 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    20701 cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    20761 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    20821 gggctggttg aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat
    20881 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    20941 cgcgataaag ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg
    21001 aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca
    21061 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg
    21121 cagggctacg acattatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    21181 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    21241 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    21301 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    21361 tacgccgggc tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc
    21421 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc
    21481 ttccgcaacg agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt
    21541 gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag
    21601 cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc
    21661 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    21721 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    21781 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    21841 accgggcaac ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc
    21901 gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac
    21961 accactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc
    22021 gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg
    22081 gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg
    22141 gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg
    22201 gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa
    22261 atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc
    22321 gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg
    22381 ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg
    22441 aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc
    22501 agctcaacat cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg
    22561 aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg
    22621 gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag
    22681 aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag
    22741 ctcagcacga tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt
    22801 ttgcccgtcg tagcaaacag cgcttggaac atctctgtga agccgaatgg gacctgctgg
    22861 tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg
    22921 ccattgaaca actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac
    22981 agctggggat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg
    23041 attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca
    23101 tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg
    23161 gcgagcagga tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga
    23221 gcgcccgtca ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt
    23281 tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc
    23341 tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta
    23401 aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg
    23461 aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc
    23521 tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc
    23581 aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta
    23641 tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac
    23701 aggtactgct gtgctcagaa atcggttctg aaggacgtaa ctttcagttc gccagtcaca
    23761 tggtgatgtt tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg
    23821 atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc
    23881 agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga
    23941 ccggacgcac tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg
    24001 atcaaaccga aggctttgac gatctgatca aaagctgccg cgagcaacat gaagcgctga
    24061 aagcacagct ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa
    24121 aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct
    24181 tcgccatgaa cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga
    24241 tcgtgctgac gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg
    24301 gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta
    24361 cctgggagca tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta
    24421 gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac
    24481 tgatttatgt ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac
    24541 cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt
    24601 ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta
    24661 acgccgtgca gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat
    24721 ctgcccgtgc attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc
    24781 tgtctcgtct ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg
    24841 ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg
    24901 atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa
    24961 ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt
    25021 ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag
    25081 cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat
    25141 ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg
    25201 ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc
    25261 ccccgcagaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca
    25321 gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc
    25381 ggcggataac acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg
    25441 tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga
    25501 agcgagagcg atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc
    25561 ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg
    25621 cagttcggta ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg
    25681 atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg
    25741 ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat
    25801 catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt
    25861 acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca
    25921 ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg
    25981 ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
    26041 ggcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg
    26101 cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc
    26161 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc
    26221 tgattttccc acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat
    26281 tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt
    26341 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
    26401 gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
    26461 gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat
    26521 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
    26581 caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa
    26641 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca
    26701 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
    26761 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
    26821 ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
    26881 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
    26941 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
    27001 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
    27061 atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg
    27121 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
    27181 ccaaagatac caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta
    27241 aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct
    27301 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
    27361 accgcgaaga acaagttgct gagatctgga acgcccgctt taaggtgggt ccggtaccga
    27421 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
    27481 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
    27541 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca
    27601 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
    27661 actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc
    27721 gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg
    27781 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
    27841 gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
    27901 ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg
    27961 cagagccgca gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta
    28021 tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg
    28081 ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg
    28141 aagcgaagtt tctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa
    28201 acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca
    28261 aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac
    28321 cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact
    28381 cgtctctgct gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg
    28441 accgtattgc ctccgctaac caagtgacga ccggtgtcac atctcgcata tatgatgatg
    28501 ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
    28561 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag
    28621 gcgacaccta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata
    28681 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    28741 gtctggtaca gctgaattac cattacgcca gcccggaata tattcaggct acgctgccta
    28801 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca
    28861 gcaggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    28921 acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg
    28981 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    29041 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc
    29101 aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac
    29161 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    29221 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    29281 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    29341 tcggtaaaac tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc
    29401 caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    29461 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    29521 aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacact
    29581 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    29641 cgtcgcatca ccatcctgcc acaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    29701 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg
    29761 acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt
    29821 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    29881 ggcggccaga tggggtgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta
    29941 agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt
    30001 ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct
    30061 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg
    30121 gagcaaattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    30181 ttctcgcagg atccaggttc tgctaaccag ggcggtgatc tcggctgggc tacaccagat
    30241 attttcgatc cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    30301 ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat
    30361 aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg
    30421 gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc
    30481 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    30541 cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg
    30601 atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt
    30661 attcccccaa ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg
    30721 cgctacgtga atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg
    30781 agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    30841 gccccgtgca taaaggcgtc attaacgacg caagcattcc gtttaccggt cataccgagt
    30901 ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    30961 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg
    31021 cacttttgca cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg
    31081 ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg
    31141 gtacggaaga gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga
    31201 aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg
    31261 acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    31321 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    31381 ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta
    31441 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    31501 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    31561 ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc
    31621 attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga
    31681 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    31741 ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt
    31801 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    31861 tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc
    31921 aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt
    31981 gatcccggta ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc
    32041 cgtcgtgcgc ctggtccctc atgcaacgat gcctcacccg gttaaagata ttcgcgtgtt
    32101 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    32161 caacctgttt agcgtcgagg tgttaaccgg aatggggatc gacccggcga tgcgagcgga
    32221 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    32281 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    32341 attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    32401 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    32461 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    32521 cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg
    32581 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    32641 ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    32701 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttaccccc
    32761 gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat
    32821 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat
    32881 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    32941 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg
    33001 ctgcaaatcg acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg
    33061 gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc
    33121 tatcccttct ttcttgacgc tatgtacggc gatatgccaa ataactggtc accggaattg
    33181 cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc
    33241 ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg
    33301 aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac
    33361 tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc
    33421 tgctggggtg ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    33481 tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatacagga
    33541 aggccggata agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaact
    33601 taccgccgct ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg
    33661 aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct
    33721 tccacttctg cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca
    33781 taaacgcgac cgccgccaat caccatgatt tctggtacgt caccacacgc cgcaatggct
    33841 tcatccaccg atttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata
    33901 atatttttgc gtcctggcaa cggacgaccg attgattccc aagtatggcg gcccataatc
    33961 acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc
    34021 atggcgtttt ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt
    34081 gagatttccc gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    34141 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctccccct
    34201 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    34261 cactctttat taagatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    34321 accttcttct gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    34381 ggtgatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    34441 gtcattctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    34501 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagcgcca gacgcccggt
    34561 tttcagcgca ccttcaaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    34621 aatgtagtga tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt
    34681 caccatctcc gtcagttgca ggttggtttg cggatcgtca atagcgttaa tcagcacttc
    34741 cgctttcgcc gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    34801 tttcatacca aatttacgca gcgtttcgat atggtccgga tcgtgatcga gcaccaccat
    34861 tttcaccccg ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac cgaatccggc
    34921 gataatcacg cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct cctccgtaga
    34981 agattgctca aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc
    35041 caccgccagg gtcagcgatt tcacccactc cggctccagc acattcgcca tctgcgccgc
    35101 accaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accagcgacg
    35161 ctgtttattt ggcacctgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    35221 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg tgccaaagtc
    35281 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa acggttcgat
    35341 atcgctttcc aacgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    35401 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacaaggaa
    35461 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg
    35521 acgcgtaaca tagcgcccca gcaataccac cagtaccagc gcacccgcca cttttaacgc
    35581 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gccgccagta gcgggatcat
    35641 cgccaccagc gggattgccg cgatatcctg gaacagcagc actgcaaagg cactgcggcc
    35701 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgttgatga
    35761 gagcgccagc gtcataccga tcaattccgc gacctgccag cgcagcccaa gtaacataca
    35821 gaacagcccc agcaggccac cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    35881 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    35941 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    36001 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    36061 taccgcaatg ggtacaatca gcgccgccga tccgaggtag atcagcgcct gaaccagcgt
    36121 atgactatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tggcgcgcct
    36181 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    36241 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    36301 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    36361 gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    36421 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga
    36481 tcagatcggc gcgagagagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    36541 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    36601 gtttattcgc atgggaatga tgcggatacg gatgcgcata aatgatgagg atcatagtta
    36661 ttagcccgcg tgtttacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct
    36721 tatagtcaat aatattgatt cagaaaagag agaaatctga acaaataatc ccattctctg
    36781 cccgcggatc gtcatattgc tggtctgcgc cagtgacatt cctcgagtct ccggggcaaa
    36841 cgctacggaa atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact
    36901 gataccgtaa ttattgataa atatcggcag tgcccaggtc gaaacaatgg tgccaatacg
    36961 acttaaggac ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt
    37021 aggatagagc cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc
    37081 catcactacc agccagatcc ccatatccgg gattagcccc aaaaccgcca acgccagcgt
    37141 catcatggca aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat
    37201 cggcggaata cagccgagca taaagaacag gctaatcacc acattcccta gtgccgcgtt
    37261 tttgccaacc cccaatccca acgaaccaac gatttgcggg ccaaaggtgt aaatggcgaa
    37321 cattgggatc acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg
    37381 gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg
    37441 ttcgccaaac agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa
    37501 ccagcgggga gattctggca gttcgaatcg accaatcaaa atcaacaaac aagggatcgc
    37561 cgcgctaccc agcatccagc gccaaccacc ttccacatca taaagccagt agccgaccag
    37621 atcggcgcag gtcgcgccga cataccacat cgcggcaata aagctgatgg aaaacgcccg
    37681 ctgacgggta ctggagaact cggtgatcat cgaagtggcg atgggataat ctgcaccgat
    37741 gacaatgccg atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa cacacatcgt
    37801 cgccaccgat atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac
    37861 tttatcggag atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc
    37921 gcccagcaag ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc
    37981 cacgccaatc attaccagta cataaccatc cagaaacgga ccaccgcttc cccacagcaa
    38041 aatgcggcgg tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg
    38101 ttattcctgt ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc
    38161 cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc
    38221 ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac
    38281 tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagca
    38341 cctgtttatc agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt
    38401 tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt
    38461 catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt
    38521 gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc
    38581 cagttccggg ttgccgctaa acatgcgtgg gttatcaagg aacgccggta gtttctggta
    38641 catgcgcata tcgcgcagcg ggccactctc cagatgctga cgatattccg cgagtttttg
    38701 cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc
    38761 ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc
    38821 atcaccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc
    38881 cggcactacg tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgcaacggc
    38941 cggatgctgt ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg
    39001 atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc
    39061 gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg
    39121 caactgaaaa cggtcttcga ttaccgactt cggtaactcg atcagttcct tcacgccaac
    39181 cgccacatcc gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt
    39241 caccccatca gcaaggagca ccgttttcgc ttcaatcaca tcaccatcgg cttctacacc
    39301 gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc
    39361 gcccgcttct tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta
    39421 agaacgctgg gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt
    39481 ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cggaatcggc
    39541 gaaacctgga ataatgtgtt ccagactgtg ggcatagaga cgcccgccag taacgttctt
    39601 ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac
    39661 cagtgcggca accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc
    39721 ggacataaga gctccctgtc agcggcagag ccgtaatgca gccctgccag agtggatcaa
    39781 cgcgctaaag ctgcggtcag tgccggaagg atcttcacgg catcgccaac aatgccgtaa
    39841 tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac
    39901 gcgttagcgc caaccatgtg ctggatctac ccggagatcc ccaccgccag gtacagttca
    39961 ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt
    40021 tctgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc
    40081 agcgcaatgt tctctttgct gccaatgccg cgaccgacgc tgaccaccag acgggctttg
    40141 tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc
    40201 ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca
    40261 tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca
    40321 atcgccagac cgccgtaaac catgtgtttc actgttgctt taccgtcctg tacgctgacg
    40381 gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat
    40441 ttgccgcgac gggtgtttgg caacagcacc agaccgtcag cgccgtgctg gcgaatagtg
    40501 tcagccatga caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc
    40561 cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag
    40621 gcattgattt gattagctaa agcctgcgca ccgttcatca gttccggcag acgagagggg
    40681 gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga
    40741 ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga
    40801 tgcgctgacg ctcgcgctgt ttcggcgcgg caacctgttg ttcagaccag gcgtccacgg
    40861 cgttaaaacc aatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa
    40921 tggctttcat cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag
    40981 gcagcggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat
    41041 ctgccgtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc
    41101 ccaccagcag accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca
    41161 gatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg
    41221 cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat
    41281 ctttacgccc tttggcgttg gtcagggctt taccgcccac acttaaggct gtcacctgcg
    41341 cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc
    41401 ttattttggc atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat
    41461 cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca
    41521 tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcacgccc tttaatttgg
    41581 aaataaaact ttaatcacca atattgaaag tcagatgcta tttttttacc cacaaaagaa
    41641 tataacaaac tgattaataa ccaaaattaa cgaaaaacgc cctctgttca ctcgaaacaa
    41701 aaatgtgata ccaatcacag aatacagctt attgaatacc cattatgagt tagccattaa
    41761 cgcgtccacg atgttaataa taattatatt aaatgttaac aaaaataaaa caaacgggaa
    41821 cgcaaaataa atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa
    41881 cgggaataga accgaaggtt ttctttccgc cattaataat cgtcggcata ctttgttggc
    41941 ttacagtcag agatctggat gcggcgaatg tcgttattaa tgctgtattc agttacgtca
    42001 ccaatgtatg gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct
    42061 ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa cgaaccgcca gaatttagca
    42121 ccgccagttg gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg
    42181 gatcgattga gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga
    42241 caggagcgaa agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg
    42301 ccacttacag cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag
    42361 tgattcgccc cagctcgaca ctggtgccgc tggcaggtga aaaacacgcc aaagggttgt
    42421 tcggtaccat cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc
    42481 tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata
    42541 ccctgcaact ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg
    42601 cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc
    42661 tgatgctggg ttgggtgttc atcgtcagcg gtgccagctt catcatgaac tacttcaccg
    42721 attcggtggg gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg
    42781 ctaaaggcgg cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttacg
    42841 ccatccagat gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt
    42901 gcttcggcat ggtgatgggg ctgacagcat ccacctggat cctgtggacc gtgctcggta
    42961 gtaacactct gctgttgatg gataaaaaca tcatcaacat tccaaatctg atcgaacagt
    43021 acggtgtggc gcgcgccatc atcgaaacct gggccgctct gccactcagc accgccacca
    43081 tgtggggctt cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt
    43141 ataccctggc gatgtctact tgccgcgaag tacgcgatgg tgaagaacca ccgctgctgg
    43201 tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg
    43261 gcctgaaacc gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca
    43321 ttatggtgac gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc
    43381 ccaaaatatc aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg
    43441 tcgccggtat ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg
    43501 accgtgacag cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca
    43561 gtctgctgat ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg
    43621 tgtggatgga gctgggacgt ctgggggcac caacctatgt actgtaccag ctgccgggcg
    43681 ggttcaatac cttcctgcgc gaaggcacac aagagcagat cgacaagatt atggctttcc
    43741 gcggcaccgg taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg
    43801 tgggtagcct gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta
    43861 agtgttttat taccagtagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg
    43921 cttctccgga caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca
    43981 aagtgaccaa acttgagaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg
    44041 acgatgtgga actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg
    44101 tcaaagaaga gttcgaccat gaacgtttcc tggtagccct gaccaactac ggtacggcga
    44161 tgtgcgcctt tgaagatgct gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta
    44221 ttggtcgttt ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca
    44281 tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg
    44341 atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa
    44401 tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg
    44461 acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat gcagatcctg acgctgggtc
    44521 gtgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc
    44581 ttatcaggcc tacgggagca cgaatgtaag ccggataagg cgtttacgcc gcatccggca
    44641 gtcatgcgct acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt
    44701 cggaccgctg gccggattgc gcgttgtctt ctccggtatc gaaatcgccg gaccgtttgc
    44761 cggacaaatg ttcgcagaat ggggcgcgga agttatttgg atcgagaacg tcgcctgggc
    44821 cgacaccatt cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct
    44881 gtcgttaaat attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac
    44941 cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga
    45001 agtactgtgg cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta
    45061 cggcaccgag gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg
    45121 ttacctgatt cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga
    45181 ttacttttct ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga
    45241 aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca
    45301 gtacttcatg atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa
    45361 agatccctac tacgccggtt gcggtctgta taaatgtgcc gacggctaca tcgtgatgga
    45421 actggtgggc attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact
    45481 cggtacgccg gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg
    45541 cccactggta gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa
    45601 agaacgcttt gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga
    45661 aagcaatcca cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg
    45721 cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg
    45781 cggaatgccc tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga
    45841 aaacgacatt caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg
    45901 cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag
    45961 gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg
    46021 tttacggtca taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata
    46081 gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga
    46141 ttcgcaaagg caacaaggtt gcactacatc tcgacaactg cccggaattt atcttttgct
    46201 ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg
    46261 aggaaagcga gtggatcttg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat
    46321 tttatcctat gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc
    46381 tgacagatgt ggcacttccc gctgatgatg gagtgagttc atttactcaa ctgaaaaatc
    46441 aacaacctgc caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc
    46501 tcttcacctc cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc
    46561 gcttcgctgg atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacatga
    46621 cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg
    46681 ccggggccac ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggcgc
    46741 agaagtaccg cgccaccatt accgaatgta ttccgatgat gatccgtacg ttgatggtgc
    46801 agccgccttc agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact
    46861 tgtcggagca ggaaaaagac gcattttgtg aacgcttcgg cgttcgcttg ctgacgtctt
    46921 atgggatgac ggaaaccatt gtgggcatta tcggcgatcg ccctagcgat aaacgacgct
    46981 ggccgtcgat tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc
    47041 gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca
    47101 tcttcaaaga gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct
    47161 ggctacatac cggcgatacc ggataccgcg acgaagaagg ctttttttat ttcgtcgatc
    47221 gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata
    47281 tcatcgccac ccatccgaaa attcaggaca tcgtggttgt gggtattaaa gattcgattc
    47341 gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg
    47401 aattttttcg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga
    47461 tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa
    47521 tgaaacgtca gggaactacc ctgccagcta ataatcacgc cctaaaacaa tatgcatttt
    47581 ttgcagggat gttgtcgtcc ctaaaaaagc aaaaatggag aaaaggaatg agtgaatcat
    47641 tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgccca aaagcgaatg
    47701 ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc
    47761 cgcaattacg tgtcgccatt attaccggtg ccggagagaa attcttttcc gcgggctggg
    47821 atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggttttg
    47881 cgggattaac cgaaattttc aatctcgaca aaccagttat cgcagctgtg aacggctacg
    47941 cctttggcgg cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg
    48001 ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc tgacagcggc ggtgtgctgc
    48061 gtctgccgaa gatcctgccg cctaccatcg tcaatgaaat ggtgatgacc ggcagacgaa
    48121 tgggcgcaga agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac
    48181 tgatggataa cgcccgcgaa ctggctcagc agctggttaa cagcgccccg ctggcgattg
    48241 cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct
    48301 atattcgcag cggcgtgttg aaacactatc catcggttct gcattcggaa gatgccattg
    48361 aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt
    48421 gagctattac gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc
    48481 cagtgccgtc ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc
    48541 ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg
    48601 ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg
    48661 gcatggagcg atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag
    48721 cgtaattatg gatggcgcgg tcattggcga agagagcatt gttgccgcaa tgagctttgt
    48781 caaagcaggc tttagtggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt
    48841 acgcagtgtt agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga
    48901 tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag ccactgaggc aaatggagga
    48961 aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat
    49021 gcgacctggt tgctggataa gatgctacaa catcgtatct ggcaagcagg acgcccggta
    49081 tcaacgagat aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg
    49141 cggttaagtt ccgtgagcgg aacggcagga atacgcggtg tctttcttac gccgactaat
    49201 ttttcccgac tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccacttta
    49261 accagtcgct gacactggca tccccgccct tccagcttat tagggatcat cttaacttcg
    49321 cagcttattt ctgtgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt
    49381 tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc
    49441 cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca
    49501 gtttcacaaa ttaaaagtct agctatgact cgttttgaag caattaaaca aggccatatt
    49561 aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct
    49621 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaaacg
    49681 gtcctcatca gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc
    49741 acgacacgct attatttgat ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc
    49801 gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc
    49861 actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct
    49921 gaggtggtgt tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga
    49981 cggccttcat gcaccttgtt taccagacgc ggattgatac ccgcttcgcc cagcacaatc
    50041 gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc
    50101 accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg
    50161 gtggagttgc tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc
    50221 atgacttccc cggtagagcg catttctggc cctaacagcg ggtcaacgcc tgggaattta
    50281 ttgaacggca gcaccacttc tttcaccgag tagtacggcg gaataacttc tttggttacg
    50341 ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg
    50401 ccggtggctt tggagacgaa cggaacagta cgcgccgcac gcgggtttac ttcaatcagg
    50461 taaacttcgt tgtttttcac cgcaaactgc acgttcatca gaccgcgcac ctgcaattcg
    50521 aaggccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtac
    50581 gctggcagcg aacatgcgga gtcaccggag tgcacacccg cctgctcgat gtgctccatg
    50641 atgccgccaa tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg
    50701 tcatcaagga aatgatccag caacactggc gcatcgttag acacgctaac cgccgtctgg
    50761 aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg
    50821 taagacggac gcaccaccag cgggtagcca atctcttttg ccttctcaac tgccatttca
    50881 atagcagtaa cggtggcgtt cgccggttgt ttcagtttca gacggtcaac cgcatgctgg
    50941 aagcgttcac ggtcttccgc acggtcgata gcatccgggc tggtgccgat aaccggtacg
    51001 ccagcggctt ccagtgcacg cgccagtttc agcggggtct gaccgccgta ctgaacgata
    51061 acgcctttcg gcttctcaat acgcacgatt tccagcacat cttccagagt taccggctcg
    51121 aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg tttccgggtt acagttaacc
    51181 ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg
    51241 aattcgatac cctgaccgat acggtttgga ccgccgccaa gcaccatgat tttttcacgg
    51301 tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta agcggtgtcg
    51361 gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg
    51421 tcacgcagct tacggatttc cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg
    51481 gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt gatgcccact
    51541 tccgccactt tctcttccag acgcaccagt tcttcaatct gtaccaggaa ccagcggtca
    51601 atgttagtca ggttgaagac gccgtccaca gacaggcccg cgcggaaagc atcggcgatg
    51661 taccagatac gctctgcgcc tgcgtctttc agttcgcgac ggattttggt taacgcttcc
    51721 gggtcatcca ggctcacttt cgggtcgaag ccagttgcgc ccacttccag gccgcgcagc
    51781 gctttttgca gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc
    51841 atctgagtgg tcagacggtc gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt
    51901 ttagtaacca catagtcgat ggacggctcg aaggaggccg gagtacgtcc gccagtgatg
    51961 tcgttcatca gttcgtctag ggtgtaaccc accgccagtt tcgccgccac tttagcaatc
    52021 gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg
    52081 ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacattgga accaccggtt
    52141 tcaacgccga tttcacgcag caccgccatc gaggcgttac gcatgatttg atattctttg
    52201 tcggtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat gcccatcgca
    52261 tcaaagtttt cgatagagca gacgatgatg cagttgtcgt ttttatcacg caccacttcc
    52321 atctcgtact ctttccagcc gatcagcgac tcatcaatca gcaactcttt ggtcggagag
    52381 agatccagac cgcgggcgca aatttcttca aactcttcac ggttataagc gataccgccg
    52441 ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagcccac gtcagcggca
    52501 acagccagcg cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt
    52561 ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc atcggcagtg
    52621 gcaccaatca tggtgacacc gaactcttcc aacacgccct gacgttccag ctccagcgcg
    52681 cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg cgtccgggcg ctctttttca
    52741 ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc
    52801 gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta accctcttcg
    52861 cgcagggctt tacatgcttg cgcgccagag tagtcaaact cacacgcctg accgataaca
    52921 atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg catggctctt
    52981 ttactcctga ttacttagcg gttttacggt actgctcaat taacttgata aagtggtcga
    53041 acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg gtgaccctgg aagctgaacg
    53101 ccggcttatc gatgcgatga atgccctgta acgtaccgtc gaacagggat ttatgcgtga
    53161 cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc tgggcggtga
    53221 tcatcaccac gtttttctcc acatctttaa ccggatggtt gccgccgtgg tgaccaaatt
    53281 tcattttgac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg agacagatgc
    53341 cgaataccgg aatatcggtt tcgaggaact tctggatggc ggtaatggcg taatcgcacg
    53401 gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt ttcagcacat
    53461 cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc accagcatcc
    53521 gcaggatgtt gcgcttggca ccaaaatcat aagccacgac gtggaacggc agctcgtctt
    53581 cttttttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc cagctatagg
    53641 cttctgcggt ggtcacttct tttgccagat ccatgccgtt cagacctggg aacgcgcggg
    53701 ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc gataatgcag ccattctgtg
    53761 cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca atcgccacga
    53821 tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg ctggcaatca
    53881 gcggcaggtc gcgaatcacc agaccttgtg catgtacctg agaagattct tcatcggcgt
    53941 cattagtgcc gacattgcca atatggggat aagtaagagt aacgatttga cgagaatagg
    54001 aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg acttccccaa
    54061 ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct tccagaacca
    54121 atagcgctga cttaatcaaa acaccctcca gagaatactc actcacttta tttgcatatt
    54181 aattcacagt gatgacgtaa atcaatgcaa atctgcttac cgacgaattt ctggcaaacg
    54241 gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcaggattt tttatattat
    54301 tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aacatcatct aacctgttct
    54361 ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa acgagcagga aaaagtggac
    54421 caaatggtca aaatttacat taaaacaaca aaaatagaag acg
//
LOCUS       DAZPFP010000010        53210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPFP010000010 DAZPFP010000000
VERSION     DAZPFP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.010"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHUR4_000526"
     CDS             complement(11..322)
                     /locus_tag="ACHUR4_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1454252.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHUR4_000527"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUR4_000527"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHUR4_000528"
     CDS             701..1054
                     /locus_tag="ACHUR4_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1454253.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUR4_000529"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUR4_000529"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR1454254.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHUR4_000530"
     CDS             complement(2810..3280)
                     /locus_tag="ACHUR4_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1454255.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUR4_000531"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUR4_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHUR4_000532"
     CDS             complement(4119..4337)
                     /locus_tag="ACHUR4_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1454256.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUR4_000533"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUR4_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1454257.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /gene="acrB"
                     /locus_tag="ACHUR4_000534"
     CDS             complement(5282..8431)
                     /gene="acrB"
                     /locus_tag="ACHUR4_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1454258.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="ACHUR4_000535"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="ACHUR4_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1454259.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="ACHUR4_000536"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="ACHUR4_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1454260.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="ACHUR4_000537"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="ACHUR4_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1454261.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="ACHUR4_000538"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="ACHUR4_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1454262.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="ACHUR4_000539"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="ACHUR4_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1454263.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="ACHUR4_000540"
     CDS             14910..15287
                     /locus_tag="ACHUR4_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1454264.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="ACHUR4_000541"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="ACHUR4_000541"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1454265.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="ACHUR4_000542"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="ACHUR4_000542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1454266.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="ACHUR4_000542"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="ACHUR4_000543"
     CDS             18098..18427
                     /locus_tag="ACHUR4_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1454267.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="ACHUR4_000544"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="ACHUR4_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1454268.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="ACHUR4_000545"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="ACHUR4_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1454269.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="ACHUR4_000546"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="ACHUR4_000546"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1454270.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="ACHUR4_000547"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="ACHUR4_000547"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1454271.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="ACHUR4_000548"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="ACHUR4_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="ACHUR4_000549"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="ACHUR4_000549"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1454272.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /gene="ybaL"
                     /locus_tag="ACHUR4_000550"
     CDS             complement(25480..27156)
                     /gene="ybaL"
                     /locus_tag="ACHUR4_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1454273.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="ACHUR4_000551"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="ACHUR4_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1454274.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="ACHUR4_000552"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="ACHUR4_000552"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1454275.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="ACHUR4_000553"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="ACHUR4_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1454276.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="ACHUR4_000554"
     CDS             complement(31204..31998)
                     /locus_tag="ACHUR4_000554"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1454277.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="ACHUR4_000555"
     CDS             32136..32477
                     /locus_tag="ACHUR4_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1454278.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="ACHUR4_000556"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="ACHUR4_000556"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1454279.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="ACHUR4_000557"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="ACHUR4_000557"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1454280.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="ACHUR4_000558"
     CDS             36393..37685
                     /locus_tag="ACHUR4_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1454281.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37716..37813
                     /locus_tag="ACHUR4_000559"
                     /pseudo
     CDS             37716..37813
                     /locus_tag="ACHUR4_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="ACHUR4_000560"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="ACHUR4_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1454282.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="ACHUR4_000561"
     CDS             complement(38218..38676)
                     /locus_tag="ACHUR4_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1454283.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="ACHUR4_000562"
     CDS             complement(38673..39590)
                     /locus_tag="ACHUR4_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1454284.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="ACHUR4_000563"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="ACHUR4_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1454285.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="ACHUR4_000564"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="ACHUR4_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1454286.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="ACHUR4_000565"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="ACHUR4_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1454287.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="ACHUR4_000566"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="ACHUR4_000566"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1454288.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="ACHUR4_000567"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="ACHUR4_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /gene="ybbA"
                     /locus_tag="ACHUR4_000568"
     CDS             43549..44235
                     /gene="ybbA"
                     /locus_tag="ACHUR4_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1454289.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /gene="ybbP"
                     /locus_tag="ACHUR4_000569"
                     /pseudo
     CDS             44232..46647
                     /gene="ybbP"
                     /locus_tag="ACHUR4_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46865..47020)
                     /gene="dinQ"
                     /locus_tag="ACHUR4_000570"
     CDS             complement(46865..47020)
                     /gene="dinQ"
                     /locus_tag="ACHUR4_000570"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1454290.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACHUR4_000571"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACHUR4_000571"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1454291.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACHUR4_000572"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACHUR4_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR1454292.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="ACHUR4_000573"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="ACHUR4_000573"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR1454293.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="ACHUR4_000574"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="ACHUR4_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR1454294.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="ACHUR4_000575"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="ACHUR4_000575"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR1454295.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="ACHUR4_000576"
     CDS             52938..>53210
                     /locus_tag="ACHUR4_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1454296.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13688 c        14016 g        12676 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgaaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagttattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DAZPFP010000011        51543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPFP010000011 DAZPFP010000000
VERSION     DAZPFP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.011"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(331..873)
                     /gene="ycfP"
                     /locus_tag="ACHUR4_000577"
     CDS             complement(331..873)
                     /gene="ycfP"
                     /locus_tag="ACHUR4_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1454297.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(896..1921)
                     /gene="nagZ"
                     /locus_tag="ACHUR4_000578"
     CDS             complement(896..1921)
                     /gene="nagZ"
                     /locus_tag="ACHUR4_000578"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1454298.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(1932..2756)
                     /gene="thiK"
                     /locus_tag="ACHUR4_000579"
     CDS             complement(1932..2756)
                     /gene="thiK"
                     /locus_tag="ACHUR4_000579"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1454299.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2737..3378)
                     /gene="lpoB"
                     /locus_tag="ACHUR4_000580"
     CDS             complement(2737..3378)
                     /gene="lpoB"
                     /locus_tag="ACHUR4_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1454300.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3393..3770)
                     /locus_tag="ACHUR4_000581"
     CDS             complement(3393..3770)
                     /locus_tag="ACHUR4_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1454301.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHUR4_000582"
     CDS             complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHUR4_000582"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1454302.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4465..6650
                     /gene="fhuE"
                     /locus_tag="ACHUR4_000583"
                     /pseudo
     CDS             4465..6650
                     /gene="fhuE"
                     /locus_tag="ACHUR4_000583"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(6710..8143)
                     /gene="ptsG"
                     /locus_tag="ACHUR4_000584"
     CDS             complement(6710..8143)
                     /gene="ptsG"
                     /locus_tag="ACHUR4_000584"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1454303.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8438..9235)
                     /locus_tag="ACHUR4_000585"
     CDS             complement(8438..9235)
                     /locus_tag="ACHUR4_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1454304.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9246..10250)
                     /gene="holB"
                     /locus_tag="ACHUR4_000586"
     CDS             complement(9246..10250)
                     /gene="holB"
                     /locus_tag="ACHUR4_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1454305.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10247..10888)
                     /gene="tmk"
                     /locus_tag="ACHUR4_000587"
     CDS             complement(10247..10888)
                     /gene="tmk"
                     /locus_tag="ACHUR4_000587"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1454306.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10878..11900)
                     /gene="yceG"
                     /locus_tag="ACHUR4_000588"
     CDS             complement(10878..11900)
                     /gene="yceG"
                     /locus_tag="ACHUR4_000588"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1454307.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11903..12712)
                     /gene="pabC"
                     /locus_tag="ACHUR4_000589"
     CDS             complement(11903..12712)
                     /gene="pabC"
                     /locus_tag="ACHUR4_000589"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1454308.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(12832..14073)
                     /gene="fabF"
                     /locus_tag="ACHUR4_000590"
     CDS             complement(12832..14073)
                     /gene="fabF"
                     /locus_tag="ACHUR4_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1454309.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14160..14396)
                     /gene="acpP"
                     /locus_tag="ACHUR4_000591"
     CDS             complement(14160..14396)
                     /gene="acpP"
                     /locus_tag="ACHUR4_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1454310.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14607..15341)
                     /gene="fabG"
                     /locus_tag="ACHUR4_000592"
     CDS             complement(14607..15341)
                     /gene="fabG"
                     /locus_tag="ACHUR4_000592"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1454311.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15354..16283)
                     /gene="fabD"
                     /locus_tag="ACHUR4_000593"
     CDS             complement(15354..16283)
                     /gene="fabD"
                     /locus_tag="ACHUR4_000593"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1454312.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16299..17252)
                     /gene="fabH"
                     /locus_tag="ACHUR4_000594"
     CDS             complement(16299..17252)
                     /gene="fabH"
                     /locus_tag="ACHUR4_000594"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1454313.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17320..18390)
                     /gene="plsX"
                     /locus_tag="ACHUR4_000595"
     CDS             complement(17320..18390)
                     /gene="plsX"
                     /locus_tag="ACHUR4_000595"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1454314.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18471..18644)
                     /gene="rpmF"
                     /locus_tag="ACHUR4_000596"
     CDS             complement(18471..18644)
                     /gene="rpmF"
                     /locus_tag="ACHUR4_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1454315.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18696..19217)
                     /gene="yceD"
                     /locus_tag="ACHUR4_000597"
     CDS             complement(18696..19217)
                     /gene="yceD"
                     /locus_tag="ACHUR4_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1454316.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19416..20000
                     /gene="yceF"
                     /locus_tag="ACHUR4_000598"
     CDS             19416..20000
                     /gene="yceF"
                     /locus_tag="ACHUR4_000598"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1454317.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20112..21071)
                     /gene="rluC"
                     /locus_tag="ACHUR4_000599"
     CDS             complement(20112..21071)
                     /gene="rluC"
                     /locus_tag="ACHUR4_000599"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1454318.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21644..24835
                     /gene="rne"
                     /locus_tag="ACHUR4_000600"
     CDS             21644..24835
                     /gene="rne"
                     /locus_tag="ACHUR4_000600"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1454319.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25032..25974)
                     /gene="flgL"
                     /locus_tag="ACHUR4_000601"
                     /pseudo
     CDS             complement(25032..25974)
                     /gene="flgL"
                     /locus_tag="ACHUR4_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(25986..27628)
                     /gene="flgK"
                     /locus_tag="ACHUR4_000602"
                     /pseudo
     CDS             complement(25986..27628)
                     /gene="flgK"
                     /locus_tag="ACHUR4_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(27694..28635)
                     /gene="flgJ"
                     /locus_tag="ACHUR4_000603"
     CDS             complement(27694..28635)
                     /gene="flgJ"
                     /locus_tag="ACHUR4_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1454320.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28635..29732)
                     /gene="flgI"
                     /locus_tag="ACHUR4_000604"
     CDS             complement(28635..29732)
                     /gene="flgI"
                     /locus_tag="ACHUR4_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1454321.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29744..30442)
                     /gene="flgH"
                     /locus_tag="ACHUR4_000605"
     CDS             complement(29744..30442)
                     /gene="flgH"
                     /locus_tag="ACHUR4_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1454322.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30495..31277)
                     /gene="flgG"
                     /locus_tag="ACHUR4_000606"
     CDS             complement(30495..31277)
                     /gene="flgG"
                     /locus_tag="ACHUR4_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR1454323.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31415..32002)
                     /gene="flgF"
                     /locus_tag="ACHUR4_000607"
                     /pseudo
     CDS             complement(31415..32002)
                     /gene="flgF"
                     /locus_tag="ACHUR4_000607"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(32104..32166)
                     /locus_tag="ACHUR4_000608"
                     /pseudo
     CDS             complement(32104..32166)
                     /locus_tag="ACHUR4_000608"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32186..33325)
                     /locus_tag="ACHUR4_000609"
     CDS             complement(32186..33325)
                     /locus_tag="ACHUR4_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1454324.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(33350..34045)
                     /gene="flgD"
                     /locus_tag="ACHUR4_000610"
     CDS             complement(33350..34045)
                     /gene="flgD"
                     /locus_tag="ACHUR4_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1454325.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34057..34460)
                     /gene="flgC"
                     /locus_tag="ACHUR4_000611"
                     /pseudo
     CDS             complement(34057..34460)
                     /gene="flgC"
                     /locus_tag="ACHUR4_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(34464..34880)
                     /gene="flgB"
                     /locus_tag="ACHUR4_000612"
     CDS             complement(34464..34880)
                     /gene="flgB"
                     /locus_tag="ACHUR4_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1454326.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35035..35694
                     /gene="flgA"
                     /locus_tag="ACHUR4_000613"
     CDS             35035..35694
                     /gene="flgA"
                     /locus_tag="ACHUR4_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1454327.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            35770..36063
                     /gene="flgM"
                     /locus_tag="ACHUR4_000614"
     CDS             35770..36063
                     /gene="flgM"
                     /locus_tag="ACHUR4_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1454328.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36068..36484
                     /gene="flgN"
                     /locus_tag="ACHUR4_000615"
     CDS             36068..36484
                     /gene="flgN"
                     /locus_tag="ACHUR4_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1454329.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(36524..38059)
                     /gene="murJ"
                     /locus_tag="ACHUR4_000616"
     CDS             complement(36524..38059)
                     /gene="murJ"
                     /locus_tag="ACHUR4_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1454330.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38226..39092)
                     /locus_tag="ACHUR4_000617"
                     /pseudo
     CDS             complement(38226..39092)
                     /locus_tag="ACHUR4_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(39094..39741)
                     /locus_tag="ACHUR4_000618"
     CDS             complement(39094..39741)
                     /locus_tag="ACHUR4_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR1454331.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39752..40336)
                     /gene="rimJ"
                     /locus_tag="ACHUR4_000619"
     CDS             complement(39752..40336)
                     /gene="rimJ"
                     /locus_tag="ACHUR4_000619"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1454332.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40572..41780
                     /gene="mdtH"
                     /locus_tag="ACHUR4_000620"
     CDS             40572..41780
                     /gene="mdtH"
                     /locus_tag="ACHUR4_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1454333.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41844..42491
                     /gene="grxB"
                     /locus_tag="ACHUR4_000621"
     CDS             41844..42491
                     /gene="grxB"
                     /locus_tag="ACHUR4_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1454334.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            42625..43185
                     /locus_tag="ACHUR4_000622"
     CDS             42625..43185
                     /locus_tag="ACHUR4_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1454335.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43291..44337
                     /gene="pyrC"
                     /locus_tag="ACHUR4_000623"
     CDS             43291..44337
                     /gene="pyrC"
                     /locus_tag="ACHUR4_000623"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1454336.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44411..44656
                     /gene="dinI"
                     /locus_tag="ACHUR4_000624"
     CDS             44411..44656
                     /gene="dinI"
                     /locus_tag="ACHUR4_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1454337.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44946..45200
                     /gene="bssS"
                     /locus_tag="ACHUR4_000625"
     CDS             44946..45200
                     /gene="bssS"
                     /locus_tag="ACHUR4_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1454338.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45315..46433
                     /gene="solA"
                     /locus_tag="ACHUR4_000626"
     CDS             45315..46433
                     /gene="solA"
                     /locus_tag="ACHUR4_000626"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1454339.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            46481..46594
                     /locus_tag="ACHUR4_000627"
     CDS             46481..46594
                     /locus_tag="ACHUR4_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR1454340.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            46855..47421
                     /locus_tag="ACHUR4_000628"
     CDS             46855..47421
                     /locus_tag="ACHUR4_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1454341.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            47425..48000
                     /locus_tag="ACHUR4_000629"
     CDS             47425..48000
                     /locus_tag="ACHUR4_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1454342.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(48042..49094)
                     /locus_tag="ACHUR4_000630"
     CDS             complement(48042..49094)
                     /locus_tag="ACHUR4_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1454343.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            49319..50239
                     /gene="lpxL"
                     /locus_tag="ACHUR4_000631"
     CDS             49319..50239
                     /gene="lpxL"
                     /locus_tag="ACHUR4_000631"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1454344.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            50411..50596
                     /locus_tag="ACHUR4_000632"
                     /pseudo
     CDS             50411..50596
                     /locus_tag="ACHUR4_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            50632..>51543
                     /locus_tag="ACHUR4_000633"
     CDS             50632..>51543
                     /locus_tag="ACHUR4_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1454345.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT        12594 a        13707 c        12728 g        12514 t
ORIGIN      
        1 caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat
       61 tgatgttaat caagttttgt tgcttaaaag ataggcaact ttaccggggg agatcaaaaa
      121 atagagttta ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat
      181 atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg
      241 actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac
      301 cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
      361 ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
      421 gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga
      481 caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc
      541 aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa
      601 attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta
      661 tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt
      721 gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
      781 caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact
      841 gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt
      901 gacctacttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt
      961 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
     1021 taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
     1081 tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat
     1141 aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa
     1201 accccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg
     1261 gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
     1321 ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc
     1381 acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg
     1441 tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt
     1501 tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca
     1561 gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac
     1621 ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg
     1681 ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta
     1741 ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact
     1801 gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca
     1861 gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca
     1921 cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata
     1981 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc
     2041 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
     2101 gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc
     2161 agttccagcg cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat
     2221 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt
     2281 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc
     2341 gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca
     2401 aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
     2461 tacgttttta ccgcaccggg cagatagtcg actaccatcc agtcacggag atataaatgc
     2521 ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg
     2581 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca
     2641 atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
     2701 aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt
     2761 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
     2821 gttaacgttg ccagaggcgc aggagtacag cacgtaatga gcgccgacat tgcgggcaat
     2881 gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt
     2941 agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt
     3001 tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct
     3061 atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat
     3121 cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg
     3181 atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg
     3241 gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc
     3301 aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta
     3361 gcgactcatt tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg
     3421 tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta
     3481 acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa
     3541 cgataatgaa cggtaacggg ttcttgcctt tcgttataaa tcgttgagga tgctgaaggt
     3601 tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat
     3661 gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc
     3721 accagcagta acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac
     3781 ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt
     3841 aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa
     3901 taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat
     3961 gctcagctga cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg
     4021 ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat
     4081 cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt
     4141 cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag
     4201 tatggtcgag ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg
     4261 ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac
     4321 attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcag
     4381 caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg
     4441 acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
     4501 caagtcatca ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct
     4561 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
     4621 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
     4681 atgactcaac atgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
     4741 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
     4801 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
     4861 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg
     4921 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
     4981 aatccatctg cggcaattaa tatggttcga aaacacgcga cctgtcgtga atttaaaggc
     5041 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
     5101 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
     5161 tcatggctgg accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat
     5221 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
     5281 ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc
     5341 gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
     5401 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
     5461 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
     5521 ctggttgggc catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg
     5581 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg
     5641 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttca
     5701 gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt
     5761 tcccacaagc cgactggtca tcacagagcc tggcgcagga cgataccaca catatgaaat
     5821 cgttatatgc tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac
     5881 gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc
     5941 cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct
     6001 ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg
     6061 gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt
     6121 tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg
     6181 gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat
     6241 ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctatc
     6301 ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa
     6361 tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact
     6421 ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag
     6481 gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac
     6541 aggggaacgt caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg
     6601 tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg
     6661 taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac
     6721 ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa atagcctgaa
     6781 caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca
     6841 ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa
     6901 tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg
     6961 cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca
     7021 gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc ggtaacagcc
     7081 acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg
     7141 taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt
     7201 ggatgatgta caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc tcggtgatac
     7261 cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt
     7321 ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca
     7381 ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc
     7441 cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt
     7501 tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa
     7561 acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa
     7621 tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga
     7681 tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga
     7741 aacggttaaa catgtacgct gcaatcgcac cggagataat ccctccgagt acgccagtat
     7801 ccgccaagtg tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca
     7861 cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat
     7921 cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga
     7981 cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg
     8041 cggaaccgac gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg
     8101 atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg
     8161 agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac
     8221 agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt
     8281 tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat
     8341 atgtcacaat atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc
     8401 gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg
     8461 aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa
     8521 cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat
     8581 tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc
     8641 gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa
     8701 tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct
     8761 ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca
     8821 gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa
     8881 tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat
     8941 aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat
     9001 cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt
     9061 tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg
     9121 ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat
     9181 gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt
     9241 ctctcttaaa gatgaggaac cggtaacaca acgcccggtt gcaggtaatg ctcaatacgc
     9301 agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca
     9361 cgaatgtggc aaacatcccc cagtatagcc tgcaggcacg agggagaaag atggtttgcc
     9421 agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc atgatggcgt
     9481 tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga
     9541 ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga
     9601 cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc
     9661 gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt
     9721 gaaagccaag tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa ccgacaacga
     9781 ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct
     9841 gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca
     9901 gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg
     9961 gtgacctcac gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg
    10021 tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt
    10081 ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca
    10141 tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag
    10201 ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact
    10261 ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg
    10321 tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg
    10381 tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg
    10441 cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtct gggcgaaaat
    10501 ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc
    10561 cctgatacgc ctgagtggaa agatcgtggc gatcgccaat cacccaggtg ccgttagcca
    10621 gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt
    10681 cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta
    10741 acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca
    10801 gttgctcgag cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc
    10861 cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc
    10921 acagacttgt tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc
    10981 acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc
    11041 ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc
    11101 gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg
    11161 gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc
    11221 tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc
    11281 tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct
    11341 ttcaccattt tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac
    11401 atcaaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc
    11461 tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc
    11521 aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct
    11581 tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg
    11641 taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc
    11701 cgtgggcgat tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc
    11761 cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg
    11821 cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata
    11881 atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat
    11941 ataacgttgc tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta
    12001 acgcattaca aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt
    12061 cgacaagctg ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt
    12121 taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat
    12181 tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt
    12241 ttgtctgctc aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac
    12301 ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt
    12361 tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc
    12421 cgctgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca
    12481 caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt
    12541 cacaggaaat catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca
    12601 aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg
    12661 cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc
    12721 cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc
    12781 taagaaaaaa ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt
    12841 tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc
    12901 cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg
    12961 gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat
    13021 agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac
    13081 caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc
    13141 gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc
    13201 tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga
    13261 cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc
    13321 accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag
    13381 tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg
    13441 ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc
    13501 tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc
    13561 aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc
    13621 atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg
    13681 gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag
    13741 gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc
    13801 aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc
    13861 catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc
    13921 agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg
    13981 accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc
    14041 cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga
    14101 ttctagtggg acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact
    14161 tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct
    14221 tcttcgtccg gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg
    14281 tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc
    14341 ttaacgccca gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc
    14401 ttaaatttcc tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa
    14461 aatttgcaac taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc
    14521 ccccaataac gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca
    14581 acatgagcat tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa
    14641 gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt
    14701 tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca
    14761 tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta
    14821 ataccgcgtg acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc
    14881 gccgcagcgt agttggcctg accgccattt cccatggtac caaccacaga accgatagtg
    14941 ataatacgac catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa
    15001 acagatgaaa ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac
    15061 aggttatcac gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg
    15121 cgaatttttt ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct
    15181 ttgccgttgg cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg
    15241 ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg
    15301 cttgcaccgg ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc
    15361 tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca
    15421 atgcgtttcg tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg
    15481 ccttgcgctg ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt
    15541 accagtgcgt cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca
    15601 acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct
    15661 gctggtttca tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt
    15721 gcgccagcag ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata
    15781 accacctgtc ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct
    15841 tcacacgctt tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg
    15901 ccttccggta cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca
    15961 gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc
    16021 atcattgccg gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca
    16081 gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt cttcagctga cccctgctgg
    16141 gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca
    16201 attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg
    16261 aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag
    16321 ccccaagtga atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc
    16381 ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg
    16441 cgatccagcg tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg
    16501 ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta
    16561 ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact
    16621 tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc
    16681 agcgtcagca attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc
    16741 tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca
    16801 cgatctgttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc
    16861 accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag
    16921 gctgctgcaa cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa
    16981 gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg
    17041 tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa
    17101 acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac
    17161 tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga
    17221 tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata
    17281 ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt
    17341 gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg
    17401 ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg
    17461 ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc
    17521 gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg
    17581 ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga
    17641 aaggaacatc ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc
    17701 acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata
    17761 attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt
    17821 tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc
    17881 ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac
    17941 gttggcccct aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac
    18001 cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag
    18061 tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag
    18121 ggccatacgc attgaactcc cacggctggc gcggatagct tgcgaaggcc gggcatcact
    18181 ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt
    18241 agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt
    18301 agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat
    18361 gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc
    18421 ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg
    18481 atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca
    18541 ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc
    18601 atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa
    18661 ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat
    18721 acggcaaatg ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct
    18781 tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt
    18841 tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca
    18901 tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt
    18961 gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc
    19021 ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat
    19081 tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta
    19141 tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt
    19201 aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa
    19261 aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat
    19321 tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg
    19381 ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca
    19441 cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac
    19501 ctgaggtcga cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg
    19561 cacaagaaaa atcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag
    19621 accaggtatg tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc
    19681 gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt
    19741 ttaattcggc gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc
    19801 acctaagcga ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg
    19861 gtagctttaa gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc
    19921 ctaacacgct ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga
    19981 aaaacccgct gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg
    20041 gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt
    20101 atatgcttgt attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc
    20161 atcggcgctt cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg
    20221 gcgtgcagga acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca
    20281 cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg
    20341 atctgatgag tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt
    20401 tcttccactt taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt
    20461 tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc
    20521 cactgaccgc gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca
    20581 tgcagagaac gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg
    20641 tcgagacgat gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg
    20701 ataacgccga agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc
    20761 accaggatat gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga
    20821 tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca
    20881 tcacccgctt cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct
    20941 ttacgcaaaa tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag
    21001 ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc
    21061 tctgttttca tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta
    21121 tgcttatttt tagttttgca ccttattact tcactacgtg atcactttat tgatggttat
    21181 taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa
    21241 gtcatcttgc tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt
    21301 gttaaaacaa gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa
    21361 tggcgtaaga cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg
    21421 gtagatggaa atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt
    21481 tttccgcatg aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg
    21541 aggttggcga cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg
    21601 cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat
    21661 caacgcaact cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga
    21721 cctggatatc gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat
    21781 cacccgcatt gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg
    21841 tttcctccca ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg
    21901 tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa
    21961 agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta
    22021 tctggttctg atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga
    22081 tgaccgtacc gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct
    22141 tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt
    22201 ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct
    22261 gattcatcag gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat
    22321 cggcgaaatc cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc
    22381 attaggtcgc ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt
    22441 cagccactac cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc
    22501 atccggtggt tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc
    22561 cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc
    22621 cgccgatgag attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga
    22681 cttcatcgac atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc
    22741 ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga
    22801 aatgtcccgc cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg
    22861 ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt
    22921 gatcgaagaa gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc
    22981 aatcgcttct tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca
    23041 ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt
    23101 gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca
    23161 tgaagaagcg atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca
    23221 acctgcactg gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc
    23281 tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc
    23341 acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga
    23401 agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca
    23461 ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg
    23521 tagtgaacgt actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc
    23581 acagcagcaa actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc
    23641 acgtaccacc gacgagcagc aagtgccgcg tcgtgaacgt agccgccgcc gtaacgatga
    23701 taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga
    23761 aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa
    23821 tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga
    23881 agaaactgcc gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt
    23941 gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc
    24001 tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg
    24061 cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat
    24121 gccgttgacc gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta
    24181 tccaattgta cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca
    24241 agtgcagccg atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc
    24301 gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca
    24361 accggaagtg gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc
    24421 gcaggtgatt accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc
    24481 gatggttgaa ccgcaggaag agacggcaga tgcagatatt gaagaagttg ccgaaactgc
    24541 tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga
    24601 agtcgcagca gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa
    24661 ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg
    24721 tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca
    24781 tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    24841 agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa
    24901 catattaaaa ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa
    24961 atgcccgccc cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt
    25021 ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct
    25081 ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag
    25141 tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag
    25201 gaatccagcg actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca
    25261 ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct
    25321 ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg
    25381 gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata
    25441 ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt
    25501 tgcttaatac tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc
    25561 tctgttttat atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc
    25621 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    25681 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    25741 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    25801 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    25861 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    25921 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    25981 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    26041 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg
    26101 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    26161 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    26221 tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    26281 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct
    26341 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    26401 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    26461 ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    26521 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    26581 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg
    26641 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg
    26701 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    26761 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    26821 atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg
    26881 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    26941 gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt
    27001 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    27061 ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt
    27121 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat
    27181 attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    27241 gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    27301 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    27361 catagcgggc ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa
    27421 acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag
    27481 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    27541 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    27601 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    27661 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    27721 gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt
    27781 acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc
    27841 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    27901 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    27961 ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga
    28021 ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg
    28081 cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg
    28141 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg
    28201 cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt
    28261 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    28321 tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt
    28381 gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg
    28441 ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg
    28501 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    28561 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    28621 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    28681 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc
    28741 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa
    28801 tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg
    28861 ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg
    28921 tcgagggtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    28981 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    29041 gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg
    29101 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    29161 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca
    29221 ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg
    29281 cctgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    29341 ctgtcaacgc ccttaagcgg agtcatcaac aacgtacctc cacgcaagct tttggcatta
    29401 cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac
    29461 gctgtcacca ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta
    29521 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    29581 tggtcaccag tgccatccag ccccactacc aagccatagc caatcagtga gttttgcctt
    29641 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    29701 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    29761 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    29821 cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    29881 tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct
    29941 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    30001 acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac
    30061 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    30121 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    30181 cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    30241 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    30301 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    30361 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    30421 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg
    30481 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    30541 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    30601 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    30661 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    30721 ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    30781 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    30841 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    30901 ccgttctgat ccacctggaa agagccgtca cggatatagg ctgatgagcc atctggcaac
    30961 attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac
    31021 agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    31081 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    31141 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    31201 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    31261 cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac
    31321 gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc
    31381 gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    31441 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    31501 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    31561 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt
    31621 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    31681 ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg
    31741 agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg
    31801 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    31861 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg
    31921 caacgcaacg tccagcgggc gagaggtgta atccatcttg ccgggcgtca tgtctgcacc
    31981 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    32041 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    32101 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    32161 atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt
    32221 cctgggtttt gatggtctgg gcgttagact tatagttacg ctgggcaacg atcatattga
    32281 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agagtgccaa
    32341 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    32401 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    32461 ccagcagttg ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca tcattgattt
    32521 gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag
    32581 cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt
    32641 ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct
    32701 gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca ttaccctgac
    32761 tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg
    32821 gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg
    32881 tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct
    32941 gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct tgcgtattca
    33001 acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt
    33061 tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg
    33121 tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc
    33181 ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg
    33241 tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc
    33301 cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct
    33361 gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt
    33421 tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac
    33481 caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag
    33541 tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg
    33601 tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt
    33661 gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg
    33721 caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg
    33781 actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc
    33841 cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg
    33901 tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg
    33961 cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat
    34021 cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt
    34081 cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc
    34141 cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta tgtagccctt
    34201 tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct gactttctta
    34261 acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc
    34321 acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc atttgccaga
    34381 ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca
    34441 aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg
    34501 atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg
    34561 gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg aaggctggtc cggaatacgg
    34621 tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgctg cgttgaggtc
    34681 atcgtcagtg caaccacact ggttgcatcc cgtccacgtt gcatgacttt tttaagttca
    34741 ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc gatgtttgct
    34801 gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa acgtaaggcg
    34861 gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca tcagcttaaa
    34921 tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat ttatgccgtt
    34981 gatggtcatt gcggacaggt acaattcacg ttgtagaaat ggctgggggc gaaaatgctg
    35041 acaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag tacggcgagc
    35101 aatctcacat cgcaattgca caactttttt agcgcccaac tcgcgggggt aagtgatgag
    35161 gttcgtgttt ctattcgtac agcgcccaat ctactaccgc catgcgagca gccattgctt
    35221 tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac
    35281 gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcgatg
    35341 cctattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg tggacgcctg
    35401 gataccctgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg
    35461 cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta
    35521 aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa
    35581 ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat ggtatcggaa
    35641 caggtagtca gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt
    35701 aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag
    35761 gaataaacca tgagtattga tcgcacttcg cctctgaagc ctgtaagcac cgttcaaccg
    35821 cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac cgcctccacc
    35881 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    35941 atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg
    36001 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    36061 tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    36121 caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca
    36181 gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca
    36241 gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga
    36301 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    36361 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    36421 gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat
    36481 ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg
    36541 caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg
    36601 ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg
    36661 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    36721 gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc
    36781 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    36841 gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    36901 caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca
    36961 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    37021 catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    37081 tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca
    37141 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    37201 aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt
    37261 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    37321 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    37381 ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    37441 gcggtagctg atacaccagc tgcagaatgc cgccgaccgt aacagcccac gccagcgcca
    37501 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa
    37561 gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    37621 ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt agctggctgg
    37681 tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    37741 gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa
    37801 cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa
    37861 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa
    37921 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    37981 ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    38041 gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg
    38101 caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt
    38161 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc
    38221 actgcctatt cgccggcggt ttgcggaact gtctgatttt acacgcattc aatgaagtgc
    38281 cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta
    38341 ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    38401 gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa
    38461 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    38521 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    38581 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    38641 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    38701 tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    38761 gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    38821 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    38881 gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga
    38941 atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt
    39001 aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    39061 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga
    39121 atcaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa
    39181 atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact
    39241 gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa
    39301 aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    39361 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa
    39421 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    39481 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    39541 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    39601 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    39661 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag
    39721 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    39781 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    39841 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    39901 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    39961 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    40021 agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt
    40081 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    40141 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    40201 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    40261 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    40321 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    40381 attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat
    40441 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tactgtcccc
    40501 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    40561 cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca
    40621 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    40681 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt
    40741 ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac
    40801 cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc
    40861 acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg
    40921 atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt
    40981 tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct
    41041 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat
    41101 gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    41161 ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc
    41221 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    41281 acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    41341 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt
    41401 tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc
    41461 tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg
    41521 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    41581 ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt
    41641 ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg ggaattattg
    41701 gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt
    41761 tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac
    41821 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    41881 tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca
    41941 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    42001 aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg
    42061 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    42121 tcaatggcta cgtcaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    42181 tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt
    42241 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg
    42301 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    42361 ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    42421 gcgttgctaa ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    42481 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    42541 ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    42601 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    42661 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    42721 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    42781 gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag
    42841 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg
    42901 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc
    42961 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    43021 caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct
    43081 tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc
    43141 attgaagtga agccaggcga aattgtcatt ccgtttacgg attaatgacg agggcgcatt
    43201 cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa
    43261 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    43321 cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg
    43381 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    43441 accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg
    43501 cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg
    43561 gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc
    43621 actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg
    43681 gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    43741 tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact
    43801 gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac
    43861 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    43921 atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt
    43981 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg
    44041 gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc
    44101 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    44161 cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac
    44221 acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat
    44281 gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    44341 tcgtagcttc ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca
    44401 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc
    44461 tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga
    44521 aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa
    44581 agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg
    44641 gtttgtcagc gaataatatg cagtgatttt tgtttttgcc ggatcgcccc ggctttttta
    44701 ttttacttac gaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat
    44761 gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat
    44821 ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt
    44881 gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg
    44941 ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca
    45001 gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt
    45061 ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac
    45121 aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa
    45181 acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc
    45241 ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga
    45301 gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg
    45361 ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc
    45421 caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg
    45481 aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat gaactttccc
    45541 gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg
    45601 actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa
    45661 agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca
    45721 tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga
    45781 tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc
    45841 gtcatgacga tgatggcgta actattgaaa cggttgacgg tgagtatcag gcgaaaaaag
    45901 cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg
    45961 tacgtaaagt atttgcctgg tatcaggccg atggccgcta tagcgtgaag aataaattcc
    46021 cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg
    46081 acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg
    46141 ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt cccgttcttg cgcaatgtat
    46201 tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg
    46261 aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga
    46321 gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag
    46381 accaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat
    46441 tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta
    46501 tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc
    46561 aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa
    46621 atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca
    46681 gcactcacca gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt
    46741 aattcaaaat tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat
    46801 tcattctccg acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca
    46861 ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca
    46921 attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat
    46981 ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg
    47041 ctggttattc gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat
    47101 tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg cattgtgcct attgctgttc
    47161 gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt
    47221 ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt
    47281 gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ctgttatgca cggatttatg
    47341 gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg
    47401 tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc
    47461 ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag
    47521 cacgcctttg ttaacttccg catacagcat cttggctata gctggttata tggcaccttt
    47581 aaagatttcg acggtacttt tacctttgat gaaaaaaatc cggctgccga taaagtgaat
    47641 gtgacaatta acaccaccag cgtcgatact aaccacgccg aacgcgataa acatcttcgc
    47701 agtgcggatt ttctcaatac cacaaaatat ccacaggcaa cattcacctc caccagcgtg
    47761 aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa
    47821 cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg cggtaaacgt
    47881 gcaggctttg aggccgaagg caaaattaag ctcaaagact tcaatatcaa gacagattta
    47941 ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa
    48001 gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga
    48061 atacccagtg ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca
    48121 cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc
    48181 tcactacaac aacctttgta tttttccgcg catactggac actgaataaa cagcaggtgg
    48241 cagccatcat ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc
    48301 gcgataatct catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca
    48361 ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc
    48421 tcgatatgcc agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa
    48481 cgaatgccgc cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg
    48541 actgcttttg gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag
    48601 tgccccactt catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca
    48661 agcatggcgt tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga
    48721 ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaag
    48781 gatttcccgt catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag
    48841 gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta
    48901 atgccctcat gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat
    48961 aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg
    49021 gttcgcggtt cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg
    49081 tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga
    49141 tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat
    49201 tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg
    49261 ggcgaaaaaa tgcgacaata catacaattg cccgtatagg ttgaaaaaca ggattgatat
    49321 gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt
    49381 gggtattggc gtactttggt tggtcgtgca attgccctac ccgattatct accgcctcgg
    49441 ttgtggatta ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg
    49501 caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa
    49561 gaatttcgaa tccgttggca tgggcctgat ggaaaccggt atggcgtggt tctggccgga
    49621 ccgccgaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc
    49681 gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc tgggcgcgcg
    49741 gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat
    49801 tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga
    49861 tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccagatca
    49921 tgattacggc ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac
    49981 cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc
    50041 acgccgtaag ccagatggca aagggtatca attgattatg ctgccaccag agtgttctcc
    50101 gccactggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat
    50161 catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg
    50221 cgttccttca cgctattaaa tctcccatgc cggatgcttc agaatggcat ccggcattac
    50281 cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg
    50341 ggcgcataat tagtgtgttt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca
    50401 gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat
    50461 cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa tgcccttctt
    50521 acccctctac gttgagcagc ttggtgttac cggccactcc gccctgaata tgtggtccgg
    50581 tattgtcttc agtataatgg gttcctaaac acatcatttt aggtattgaa tatgagacgg
    50641 gcaatcagct acatcagatt ttcaagtgaa cgacaactga aaggggactc tgtacgccga
    50701 caatctaagc tggttactga ctggcttgat aagaaccctg agttttactt ggattcatcc
    50761 ttgagcttta aagatttggg gaaaagtgct tttagtggta agcacctgaa aggtggtcta
    50821 ggtgactttt taaccgctat cgagaaaggg cttgttaaag ctggtgatac gctactaatc
    50881 gagagtttag accgtctgtc acgtcaggat attgatattg cttctgagct attacgccgt
    50941 atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta cacacgagaa
    51001 agtttaaaag acccgttagc cctgattaaa agcattctga taatgcagcg ggcgcatgag
    51061 gaaagtttga gaaagtctga gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc
    51121 tccgaaggta tcaaggtatc taggcgttgt cctgcatggt taaggctgaa tgatgaccgt
    51181 cgaacattca caatcattcc tgataaagtg gaagttgtta aacgagcatt tgacctgaga
    51241 ttacagggtt taagcttttg ggctatcacc agaactttaa acgatgaagg tcacttgtca
    51301 ttgaatcaat acacacccaa acaaaagggg tggtctgata ctgctgtaaa gaaactgctc
    51361 agaaatcgtg ctgtgattgg atgtttcaca cctgctggac gtgaagaagt tcagggttac
    51421 taccccgcaa taatcagtga gtcactattc tatcgagtac agcaactgaa cacagggcaa
    51481 tatggtaggg cttctgtatc aagtaatccg ctatcagtca acttgttcag gggtatcatc
    51541 aaa
//
LOCUS       DAZPFP010000012        49532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPFP010000012 DAZPFP010000000
VERSION     DAZPFP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.012"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUR4_000634"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUR4_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1454346.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUR4_000635"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUR4_000635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1454347.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUR4_000636"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUR4_000636"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1454348.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUR4_000637"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUR4_000637"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1454349.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHUR4_000638"
     CDS             5718..6659
                     /locus_tag="ACHUR4_000638"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1454350.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUR4_000639"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUR4_000639"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1454351.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHUR4_000640"
     CDS             7679..8929
                     /locus_tag="ACHUR4_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1454352.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHUR4_000641"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHUR4_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUR4_000642"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUR4_000642"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1454353.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHUR4_000643"
     CDS             10866..12116
                     /locus_tag="ACHUR4_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1454354.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHUR4_000644"
     CDS             complement(12172..13260)
                     /locus_tag="ACHUR4_000644"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1454355.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUR4_000645"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUR4_000645"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1454356.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHUR4_000646"
     CDS             complement(14194..15243)
                     /locus_tag="ACHUR4_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1454357.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUR4_000647"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUR4_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1454358.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUR4_000648"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUR4_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1454359.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUR4_000649"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUR4_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUR4_000650"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUR4_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHUR4_000651"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHUR4_000651"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1454360.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUR4_000652"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUR4_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1454361.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHUR4_000653"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHUR4_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1454362.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUR4_000654"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUR4_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1454363.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUR4_000655"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUR4_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1454364.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUR4_000656"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUR4_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1454365.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUR4_000657"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUR4_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUR4_000658"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUR4_000658"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR1454366.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="ACHUR4_000659"
     CDS             complement(30809..31048)
                     /locus_tag="ACHUR4_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1454367.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHUR4_000660"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHUR4_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1454368.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHUR4_000661"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHUR4_000661"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1454369.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="ACHUR4_000662"
     CDS             complement(32934..33629)
                     /locus_tag="ACHUR4_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1454370.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="ACHUR4_000663"
     CDS             complement(33626..34024)
                     /locus_tag="ACHUR4_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1454371.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="ACHUR4_000664"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="ACHUR4_000664"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1454372.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHUR4_000665"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHUR4_000665"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1454373.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHUR4_000666"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHUR4_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37416..38138
                     /locus_tag="ACHUR4_000667"
                     /pseudo
     CDS             37416..38138
                     /locus_tag="ACHUR4_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="ACHUR4_000668"
     CDS             complement(38255..38833)
                     /locus_tag="ACHUR4_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1454374.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="ACHUR4_000669"
     CDS             39003..39590
                     /locus_tag="ACHUR4_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1454375.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHUR4_000670"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHUR4_000670"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1454376.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHUR4_000671"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHUR4_000671"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1454377.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="ACHUR4_000672"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="ACHUR4_000672"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1454378.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="ACHUR4_000673"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="ACHUR4_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1454379.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="ACHUR4_000674"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="ACHUR4_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1454380.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="ACHUR4_000675"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="ACHUR4_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1454381.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="ACHUR4_000676"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="ACHUR4_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1454382.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="ACHUR4_000677"
     CDS             complement(48911..49018)
                     /locus_tag="ACHUR4_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1454383.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49532)
                     /locus_tag="ACHUR4_000678"
                     /pseudo
     CDS             complement(49078..49532)
                     /locus_tag="ACHUR4_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12203 c        13311 g        12063 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttattg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgctgcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc cg
//
LOCUS       DAZPFP010000013        48548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPFP010000013 DAZPFP010000000
VERSION     DAZPFP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.013"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            144..1361
                     /gene="alaA"
                     /locus_tag="ACHUR4_000679"
     CDS             144..1361
                     /gene="alaA"
                     /locus_tag="ACHUR4_000679"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1454384.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1445..2044
                     /gene="yfbR"
                     /locus_tag="ACHUR4_000680"
     CDS             1445..2044
                     /gene="yfbR"
                     /locus_tag="ACHUR4_000680"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1454385.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2103..3935)
                     /locus_tag="ACHUR4_000681"
     CDS             complement(2103..3935)
                     /locus_tag="ACHUR4_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1454386.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4022..4672)
                     /gene="hxpA"
                     /locus_tag="ACHUR4_000682"
     CDS             complement(4022..4672)
                     /gene="hxpA"
                     /locus_tag="ACHUR4_000682"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR1454387.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4683..5177)
                     /locus_tag="ACHUR4_000683"
     CDS             complement(4683..5177)
                     /locus_tag="ACHUR4_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1454388.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5260..5715)
                     /gene="yfbV"
                     /locus_tag="ACHUR4_000684"
     CDS             complement(5260..5715)
                     /gene="yfbV"
                     /locus_tag="ACHUR4_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1454389.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6053..7255
                     /gene="ackA"
                     /locus_tag="ACHUR4_000685"
     CDS             6053..7255
                     /gene="ackA"
                     /locus_tag="ACHUR4_000685"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1454390.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7330..9474
                     /gene="pta"
                     /locus_tag="ACHUR4_000686"
     CDS             7330..9474
                     /gene="pta"
                     /locus_tag="ACHUR4_000686"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1454391.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9664..11184
                     /gene="yfcC"
                     /locus_tag="ACHUR4_000687"
     CDS             9664..11184
                     /gene="yfcC"
                     /locus_tag="ACHUR4_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1454392.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11217..11759)
                     /gene="yfcD"
                     /locus_tag="ACHUR4_000688"
     CDS             complement(11217..11759)
                     /gene="yfcD"
                     /locus_tag="ACHUR4_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1454393.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11817..12368)
                     /gene="yfcE"
                     /locus_tag="ACHUR4_000689"
     CDS             complement(11817..12368)
                     /gene="yfcE"
                     /locus_tag="ACHUR4_000689"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1454394.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12424..13068)
                     /gene="yfcF"
                     /locus_tag="ACHUR4_000690"
     CDS             complement(12424..13068)
                     /gene="yfcF"
                     /locus_tag="ACHUR4_000690"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1454395.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13204..13851
                     /gene="yfcG"
                     /locus_tag="ACHUR4_000691"
     CDS             13204..13851
                     /gene="yfcG"
                     /locus_tag="ACHUR4_000691"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1454396.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13908..14270
                     /gene="folX"
                     /locus_tag="ACHUR4_000692"
     CDS             13908..14270
                     /gene="folX"
                     /locus_tag="ACHUR4_000692"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1454397.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14291..15184
                     /locus_tag="ACHUR4_000693"
     CDS             14291..15184
                     /locus_tag="ACHUR4_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1454398.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15232..16122)
                     /gene="rpnB"
                     /locus_tag="ACHUR4_000694"
     CDS             complement(15232..16122)
                     /gene="rpnB"
                     /locus_tag="ACHUR4_000694"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1454399.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16319..17092)
                     /gene="hisP"
                     /locus_tag="ACHUR4_000695"
     CDS             complement(16319..17092)
                     /gene="hisP"
                     /locus_tag="ACHUR4_000695"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1454400.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17100..17816)
                     /locus_tag="ACHUR4_000696"
     CDS             complement(17100..17816)
                     /locus_tag="ACHUR4_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454401.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17813..18499)
                     /gene="hisQ"
                     /locus_tag="ACHUR4_000697"
     CDS             complement(17813..18499)
                     /gene="hisQ"
                     /locus_tag="ACHUR4_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1454402.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18589..19371)
                     /gene="hisJ"
                     /locus_tag="ACHUR4_000698"
     CDS             complement(18589..19371)
                     /gene="hisJ"
                     /locus_tag="ACHUR4_000698"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1454403.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19592..20374)
                     /gene="argT"
                     /locus_tag="ACHUR4_000699"
     CDS             complement(19592..20374)
                     /gene="argT"
                     /locus_tag="ACHUR4_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1454404.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20640..21209)
                     /gene="ubiX"
                     /locus_tag="ACHUR4_000700"
     CDS             complement(20640..21209)
                     /gene="ubiX"
                     /locus_tag="ACHUR4_000700"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1454405.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21304..22821)
                     /gene="purF"
                     /locus_tag="ACHUR4_000701"
     CDS             complement(21304..22821)
                     /gene="purF"
                     /locus_tag="ACHUR4_000701"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1454406.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22858..23346)
                     /gene="cvpA"
                     /locus_tag="ACHUR4_000702"
     CDS             complement(22858..23346)
                     /gene="cvpA"
                     /locus_tag="ACHUR4_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1454407.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23605..24267)
                     /gene="dedD"
                     /locus_tag="ACHUR4_000703"
     CDS             complement(23605..24267)
                     /gene="dedD"
                     /locus_tag="ACHUR4_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1454408.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24257..25525)
                     /gene="folC"
                     /locus_tag="ACHUR4_000704"
     CDS             complement(24257..25525)
                     /gene="folC"
                     /locus_tag="ACHUR4_000704"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1454409.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25595..26509)
                     /gene="accD"
                     /locus_tag="ACHUR4_000705"
     CDS             complement(25595..26509)
                     /gene="accD"
                     /locus_tag="ACHUR4_000705"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1454410.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26665..27324)
                     /locus_tag="ACHUR4_000706"
     CDS             complement(26665..27324)
                     /locus_tag="ACHUR4_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1454411.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27407..28219)
                     /gene="truA"
                     /locus_tag="ACHUR4_000707"
     CDS             complement(27407..28219)
                     /gene="truA"
                     /locus_tag="ACHUR4_000707"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1454412.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28219..29232)
                     /locus_tag="ACHUR4_000708"
     CDS             complement(28219..29232)
                     /locus_tag="ACHUR4_000708"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1454413.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29298..30434)
                     /gene="pdxB"
                     /locus_tag="ACHUR4_000709"
     CDS             complement(29298..30434)
                     /gene="pdxB"
                     /locus_tag="ACHUR4_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1454414.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30533..31528
                     /gene="flk"
                     /locus_tag="ACHUR4_000710"
     CDS             30533..31528
                     /gene="flk"
                     /locus_tag="ACHUR4_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1454415.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31525..32703)
                     /locus_tag="ACHUR4_000711"
     CDS             complement(31525..32703)
                     /locus_tag="ACHUR4_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454416.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32958..34199)
                     /gene="fabB"
                     /locus_tag="ACHUR4_000712"
     CDS             complement(32958..34199)
                     /gene="fabB"
                     /locus_tag="ACHUR4_000712"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1454417.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34358..36364
                     /gene="mnmC"
                     /locus_tag="ACHUR4_000713"
     CDS             34358..36364
                     /gene="mnmC"
                     /locus_tag="ACHUR4_000713"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1454418.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36485..36763)
                     /locus_tag="ACHUR4_000714"
     CDS             complement(36485..36763)
                     /locus_tag="ACHUR4_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1454419.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(36797..37345)
                     /gene="epmC"
                     /locus_tag="ACHUR4_000715"
     CDS             complement(36797..37345)
                     /gene="epmC"
                     /locus_tag="ACHUR4_000715"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1454420.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37345..38154)
                     /locus_tag="ACHUR4_000716"
     CDS             complement(37345..38154)
                     /locus_tag="ACHUR4_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR1454421.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38154..38978)
                     /gene="mepA"
                     /locus_tag="ACHUR4_000717"
     CDS             complement(38154..38978)
                     /gene="mepA"
                     /locus_tag="ACHUR4_000717"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1454422.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38982..40067)
                     /gene="aroC"
                     /locus_tag="ACHUR4_000718"
     CDS             complement(38982..40067)
                     /gene="aroC"
                     /locus_tag="ACHUR4_000718"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1454423.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40102..41034)
                     /gene="prmB"
                     /locus_tag="ACHUR4_000719"
     CDS             complement(40102..41034)
                     /gene="prmB"
                     /locus_tag="ACHUR4_000719"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1454424.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41200..41751
                     /gene="smrB"
                     /locus_tag="ACHUR4_000720"
     CDS             41200..41751
                     /gene="smrB"
                     /locus_tag="ACHUR4_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1454425.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41855..42745)
                     /locus_tag="ACHUR4_000721"
     CDS             complement(41855..42745)
                     /locus_tag="ACHUR4_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1454426.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(42732..43256)
                     /locus_tag="ACHUR4_000722"
     CDS             complement(42732..43256)
                     /locus_tag="ACHUR4_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1454427.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43253..43723)
                     /locus_tag="ACHUR4_000723"
     CDS             complement(43253..43723)
                     /locus_tag="ACHUR4_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1454428.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(43720..44268)
                     /locus_tag="ACHUR4_000724"
     CDS             complement(43720..44268)
                     /locus_tag="ACHUR4_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1454429.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44273..44995)
                     /locus_tag="ACHUR4_000725"
                     /pseudo
     CDS             complement(44273..44995)
                     /locus_tag="ACHUR4_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45015..47655)
                     /gene="yfcU"
                     /locus_tag="ACHUR4_000726"
                     /pseudo
     CDS             complement(45015..47655)
                     /gene="yfcU"
                     /locus_tag="ACHUR4_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47737..48300)
                     /locus_tag="ACHUR4_000727"
     CDS             complement(47737..48300)
                     /locus_tag="ACHUR4_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1454430.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12048 a        13037 c        11983 g        11480 t
ORIGIN      
        1 agttattaac agcacaatcg aaccttcccc tctggcaaaa tcttattctg cagaccttca
       61 aaacaccgtc ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat
      121 aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg
      181 tctgttatga catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta
      241 acaaggtact gaaactgaac atcggcaacc cagccccatt cggttttgac gcgccagatg
      301 aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca agggtattgc gattccaaag
      361 ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg
      421 ttaccgtgga agatatttac atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc
      481 aggcattgct gaacagcggg gacgaaatgt tggttcctgc accagattac ccactctgga
      541 ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg
      601 actggttccc ggacctcgat gatattcgcg ctaaaattac gcctcgtacg cgtgggattg
      661 ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga
      721 ttgtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa
      781 ttctctacga cgatgctgag catcactcaa ttgcgccgct ggcacctgac ctgctgacca
      841 ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg
      901 tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt
      961 caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt
     1021 atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg
     1081 cgtgggaact gatcaacgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt
     1141 atatgttccc gaaaatcgac gccaaacgct ttaacattca tgacgatcag aaaatggtgc
     1201 tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc aactggccgt
     1261 ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga
     1321 gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga
     1381 gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt
     1441 cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg
     1501 gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat
     1561 ggtcgcccat gcgctggcag ctatcaaaaa tcggaaattt ggcggtaatg tcaacgccga
     1621 acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc
     1681 tactccggtg aaatacttca atgcgcaaat cgctcaggag tacaaggcta ttgaaaaaat
     1741 cgctcagcag aaactggtcg atatggttcc ggaagaactg cgggatatct ttgcgccgtt
     1801 aattgacgag catgcatata gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact
     1861 gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga aataatgaat tcttgctggc
     1921 aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc caggagatgg actacttcat
     1981 ggaagtattt gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct
     2041 gtaagcagcc ggagtctgcg tcgcatcagt caataagcgc cggatgcgac atcaggctct
     2101 tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg
     2161 aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagg
     2221 gtgttaacag gtgaagacac cggtgtcata aaggcggcgg atgctgccat cgccacgacc
     2281 atcgcaaatg gataaggcga cactcccatc gttttggcag cagccagcgc aatcggagcc
     2341 atcaacaccg ccgtcgcggt attagagata aacagcccaa taaccgccga caagacaaac
     2401 aaacacccca gcatcatatg tggcccataa ccgccgccaa tatccatcag ccctttcacc
     2461 gccagcgcga cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc
     2521 aaaataatgc tcggccagtg aatggattta taggcgcttt cagcatctat acagcggaat
     2581 ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc atttgtcagc
     2641 attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctggct gtgcgcgggc
     2701 gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc
     2761 agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc
     2821 tcatccgcca gcgaaccttc cagcgccacg ccattgcgct tgagccccac cacattcagt
     2881 ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caatcagttc tgactcagga
     2941 attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc
     3001 agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca
     3061 gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg
     3121 ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcgcc ataacgctca
     3181 cgtaatttga gatcatccag ccgttgacct atcattggcg atccggggcg aatagccaga
     3241 cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt tcgacgcgtc
     3301 cagccttcgc gctgcggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata
     3361 tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag
     3421 ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc
     3481 agcgtcatca tgccgctaat cagcccggca aaacttaacg gcatcatcag acgtgacggc
     3541 gaagtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgctg
     3601 gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg
     3661 atttcactgt tgcccgccac tttgaccagc catgttccca ttacggtggc aacaccggta
     3721 cggaccaaac catcgccaat aataaacaag gcggcaatca ggacaacgtt aggatcagaa
     3781 aagccggaaa atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca
     3841 aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc
     3901 agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg
     3961 atgacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt
     4021 ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt
     4081 ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga
     4141 catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc accaccacac
     4201 actcctgcgg cgcaagcccc agcagctgcg cacctaacag atacgcatca ggttctggtt
     4261 ttccgcgctt cactcgctca gcggttacaa acacctctgg tgcgggaagc cccgctattt
     4321 tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg
     4381 ctttattcaa atgattgagt aaggcgattg ccccaggaag cgcggtaata ccttcggttt
     4441 ccgtggcctc gatttgctcc agacgcgtaa actcggcggc aatatcagcc tcggatttgc
     4501 ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct
     4561 cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca
     4621 gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct
     4681 cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag
     4741 gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa
     4801 ccatgagtcc cagcgtcaaa atgggtatag cgcccttcca cattaaccat aaagcggaca
     4861 taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca
     4921 tcgatggatt gctggtcctg caaattagac caagaaacat acaacgcatg atacatatcc
     4981 ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccaat
     5041 tcacgcatct gtaatccgta accacgctca ataattgttt gcaggcgacg gtaacgttca
     5101 gcatttgccg gatcgagcat agtcatcatc ttgtactgat tagacaaaat aagacgttgc
     5161 gcgttggtca tttccattgt tgactcctgt atcactctac tacggtgaaa aaaaagaagg
     5221 ctgagtatgc cttcttttat atgcgtaatc aggggtcaat tacaaatcat caaggaaagt
     5281 tttatccagt tgtttgaagg cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt
     5341 gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa
     5401 ccagttaagg attgcagggg gtaatggcgt tacagaacgc ttgcccagcc accacaatcc
     5461 ctgcatgggt aaacttaagg cgaacagtgc agtggcaact gccggcccaa gcagaccgcc
     5521 cagggcaatc tgccagcaga gagtaaatac ggcgatcggc ggcataaaac ggatcgcata
     5581 acgcgtcatc ttgataacgc gattttcgac aaagaccgga gcaaggcgtt tttccagtgg
     5641 ccacgtcttt gagtaatgct gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt
     5701 atccggcgtt gacatgcttc acctcaactt cacatataaa gattcaaaaa tttgtgcaaa
     5761 ttcacaactc agcgggacaa cgttcaaaac attttgtctt ccatacccac tatcaggtat
     5821 cctttagcag cctgaaggcc taagtagtac atattcattg agtcgtcaaa ttcatataca
     5881 ttatgccatt ggctgaaaat tacgcaaaat ggcatagact caagatattt cttccatcat
     5941 gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc atgcgctacg
     6001 ctctatggct ccctgacgtt tttttagcca cgtatcaatt ataggtactt ccatgtcgag
     6061 taagttagta ctggttctga actgcggtag ttcttcactg aaatttgcca tcatcgatgc
     6121 agtaaatggt gaagagtacc tttctggttt agccgaatgt ttccacctgc ccgaagcacg
     6181 tatcaaatgg aaaatggacg gcaataaaca ggaagcggct ttaggtgcag gcgccgctca
     6241 cagcgaagcg ctcaacttta tcgttaatac tattctggca caaaaaccag aactgtctgc
     6301 gcagctgact gctatcggtc accgtatcgt acacggcggc gaaaagtata ccagctccgt
     6361 agtgatcgat gagtctgtta ttcagggtat caaagatgca gcttcttttg caccgctgca
     6421 caacccggct cacctgatcg gtatcgaaga agctctgaaa tctttcccac agctgaaaga
     6481 caaaaacgtt gctgtatttg acaccgcgtt ccaccagact atgccggaag agtcttacct
     6541 ctacgccctg ccgtacaacc tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg
     6601 caccagccac ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga
     6661 actgaacatc atcacctgcc acctgggcaa cggtggttcc gtttctgcta tccgcaacgg
     6721 taaatgcgtt gacacctcta tgggtctgac cccgctggaa ggtctggtca tgggtacccg
     6781 ttctggtgat atcgatccgg cgatcatctt ccacctgcac gacaccctgg gcatgagcgt
     6841 tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac
     6901 cagcgactgc cgctatgttg aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat
     6961 ggacgtttac tgccaccgcc tggcgaaata catcggtgcc tacactgcgc tgatggatgg
     7021 tcgtctggac gctgttgcat tcactggtgg tatcggtgaa aatgccgcga tggttcgtga
     7081 actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc
     7141 tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg cggtggttat
     7201 cccaaccaac gaagaactgg ttatcgcgca agacgcgagc cgcctgactg cctgatttca
     7261 caccgccagc tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga
     7321 ggataaaccg tgtcccgtat tattatgctg atccctaccg gaaccagcgt cggtctgacc
     7381 agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc
     7441 aaacctatcg ctcagccgcg taccggtggc gatgcgcccg atcagactac gactatcgtg
     7501 cgtgcgaact cttccaccac gacggccgct gaaccgctga aaatgagcta cgttgaaggt
     7561 ctgctttcca gcaatcagaa agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct
     7621 aacaccaaag acgctgaagt cgttctggtt gaaggtctgg tcccgacacg taagcaccag
     7681 tttgcccagt ctctgaacta cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt
     7741 atgtctcagg gcactgacac cccggaacag ctgaaagagc gtatcgaact gacccgcaac
     7801 agcttcggtg gtgccaaaaa caccaacatc accggcgtta tcgttaacaa actgaacgca
     7861 ccggttgatg aacagggtcg tactcgcccg gatctgtccg agattttcga cgactcttcc
     7921 aaagctaaag taaacaatgt tgatccggcg aagctgcaag aatctagccc gctgccggtt
     7981 ctcggcgctg tgccgtggag ctttgacctg atcgcgactc gtgcgatcga tatggctcgc
     8041 cacctgaatg cgaccatcat caacgaaggc gacatcaata ctcgccgcgt taaatccgtc
     8101 actttctgcg cacgcagcat tccgcacatg ctggagcact tccgtgccgg ttctctgctg
     8161 gtgacttccg cagaccgtcc tgacgtgctg gtggccgctt gcctggcagc catgaacggc
     8221 gtagaaatcg gtgccctgct gctgactggc ggctacgaaa tggacgcgcg catttctaaa
     8281 ctgtgcgaac gtgctttcgc taccggcctg ccggtattta tggtgaacac caacacctgg
     8341 cagacctctc tgagcctgca gagcttcaac ctggaagttc cggttgacga tcacgaacgt
     8401 atcgagaaag ttcaggaata cgttgctaac tacatcaacg ctgactggat cgaatctctg
     8461 actgccactt ctgagcgcag ccgtcgtctg tctccgccag cgttccgtta tcagctgact
     8521 gaactggcgc gcaaagcggg caaacgtatc gttctgccgg aaggtgacga accgcgtacc
     8581 gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat
     8641 ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag aactaggtgc aggcattgaa
     8701 atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc gtctggtcga gctgcgtaag
     8761 aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt
     8821 acgctgatgc tggaacagga tgaagttgat ggtctggttt ccggtgctgt tcacactacc
     8881 gcaaacacca tccgtccgcc gctgcagctg atcaaaactg caccgggcag ctccctggta
     8941 tcttccgtgt tcttcatgct gctgccggaa caggtttacg tttacggtga ctgtgcgatc
     9001 aacccggatc cgaccgctga acagctggca gaaatcgcga ttcagtccgc tgattccgct
     9061 gcggccttcg gtatcgaacc gcgcgttgct atgctctcct actccaccgg tacttctggt
     9121 gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct
     9181 gacctgatga tcgacggtcc gctgcagtac gacgctgcgg taatggctga cgttgcgaaa
     9241 tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg tgttcatctt cccggatctg
     9301 aacaccggta acaccaccta caaagcggta cagcgttctg ccgacctgat ctccatcggg
     9361 ccgatgctgc agggtatgcg caaaccggtt aacgacctgt cccgtggcgc actggttgac
     9421 gatatcgtct acaccatcgc gctgactgcg attcagtctg cacagcagca gtaatctcgt
     9481 catcatccac agctttgcgc tgcggatatc tgaaccggaa ataatcacta tttccggttt
     9541 tttattctct taatttgcat taatcctttc tgattatctt gcttaactgc gctgcatcaa
     9601 tgaattgcgc catctcactt tgcatactta ccactttgtt ttgtgcaagg gaatatttgc
     9661 gctatgtccg caatcactga atccaaacca acaagaagat gggcaatgcc cgatacgttg
     9721 gtgattatct tttttgttgc tattttaacc agtcttgcca cctgggtagt tccggtgggg
     9781 atgtttgaca gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc
     9841 gtagatccac actcatttcg cattctgact aacgaagcag gcgaacctga gtatcaccgc
     9901 gtacagctgt tcacgacggg cgatgaacgc ccgggcctga tgaacttccc gtttgaaggg
     9961 ttaacctcag gatcgaaata cgggacagcc gttggcatca tcatgtttat gctggtgatt
    10021 ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg ataacggtat cctggcgctt
    10081 attcgccata cccgcgggaa tgaaattctc tttattcctg cgctgtttat tctgttttca
    10141 cttggcggcg cggtatttgg tatgggagaa gaggccgtcg cctttgccat tatcatcgca
    10201 ccgctaatgg tccggctggg ctatgacagt attaccaccg tcctggtgac ttatattgcc
    10261 acgcaaatcg gttttgccag ttcgtggatg aacccgtttt gtgtggttgt tgctcagggg
    10321 attgccggcg ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc
    10381 actctgattg gcctgatctt taccatggtg tacgcctcac gagtgaaaaa gaatcctctt
    10441 ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa
    10501 cgtccgttta cctttggtga ctggctggta ttgattgtcc tgacagccgt aatggtctgg
    10561 gtgatttggg gcgtgatcgt taatgcctgg tttattccag aaattgccag ccagttcttc
    10621 accatgggtc tggtgattgg catcatcggc gttgttttcc gccttaacgg catgacggtt
    10681 aataccatgg cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg
    10741 gtgggtttcg ccaaagggat tttgctgctg gttggtaatg gtgaagcagg taatgccagc
    10801 gtgttaaata ctatcctcaa cagcattgcc aatgccatta gcggtctgga caacgcggtc
    10861 gcggcctggt ttatgttgct cttccaggct gtatttaatt tcttcgtgac gtccggttct
    10921 ggtcaggcgg cattaactat gccgttactg gcaccgcttg gcgatctggt cggtgttaac
    10981 cgtcaggtta ccgtgctggc gttccagttt ggtgatggct tcagccacat catttaccca
    11041 acctcagctt cgttaatggc aacgctcggt gtgtgccggg tggacttccg taactggctg
    11101 aaggtgggcg cgaccctgct tggactgctg tttattatgt ccagcgtcgt agtgatcggc
    11161 gctcagttga tgggctacca ctaaaaatgt taagagccgc aattgcggct cttttttcat
    11221 tctgccgttt cagtctctac ggcttcattt ttggcattgc gcttcatcca caacgccagc
    11281 gctttcagcg agtctggagt gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc
    11341 cagcaaactc cactgacttc atcttcctgt agggcgaagg gaccgtgaga gacgcaactg
    11401 aacaacgcgc cccagacacg gcaattttta tcttcgaaat agaactgccc gtgctcggca
    11461 aaggggacac cggcaatgcc caactcttct tccgcttcgc gacgcgcgga ttccagcagt
    11521 tgctcatcgg cctgaactac accgcctgcg gtcgcatcta acatgccggg taaaaagtct
    11581 tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc catcatgcac gacgatgtaa
    11641 gttgcacgat gacgcagaca ctgtgcccgc atttgttccc ggctggcttg tgcaatgact
    11701 tcgttctctt cattgacaat atccacccat tcagtacttg ccaaacgacg ctgttccatt
    11761 atcaggaaac cttctttttc tggcgctctt acggcgcatt ttgagttgtg ggtaaattac
    11821 ggattaatcg cgacctgcgc aatgatactt tgatcattga gtgcgattac actaagtacg
    11881 tcattatcca gcatgccata acccgccgga ttaccgcctt tcggaatact caccgagccg
    11941 gggttgaagt ggaaaatctc cccccgctgt tctgccactg gcagatgggt atgaccgtac
    12001 accagcacat cgttctggtt taaagcaggt agattttccg ggccaaaaag atgaccatgc
    12061 gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc acggcgcggt tatcgggaaa
    12121 tgcagcatca tttgatccac ttcgctgtcg cagttgccac gcacagcgat aaccttatgt
    12181 gccacttcat taagccgttc agcgactttg gctggcgcgt aaccctccgg taaagcatta
    12241 cgcgggccgt gattcaacac gtcgccaagg atcaccagcc actgggcacc gctttggaca
    12301 aacaactcca caacacgttc cgtcgccggt aacgacccat gaatgtccga tgcaaacatc
    12361 agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg
    12421 ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca
    12481 ctggaatgtc gcataatcca ccaggcgttc tggcacctca tcgccatgta gtaccaggcg
    12541 gttaatcatt agcgccagat cagtatcagc aatgcaccat tcaccaaata aattcggctg
    12601 acccagtgct aacaaatgtt ctgccatcgc gaacagtttc tctgcactgg cttttccctc
    12661 ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg
    12721 gatgggcatc agatcgctgc gcagccaggc ctgaatctgt cgtgcacgcg cacgattttc
    12781 taaatcaagc ggataaatac gttcccaggt cggtggcgca aatcgatctt ccagatactc
    12841 cgcaatggca gaagattcac tcaactcaaa atcatcgatt tgtaataacg gcacacggcg
    12901 tgtctgaccg taaccttgcc acgtcggctg caaatgttca ccgctgtcga ggtcgatggt
    12961 cttgatatga aacgacaggc ctttttcctg caacgccacc caggcggata acacataagg
    13021 ggagaaaaag tgggcatctg accaaagcgt gatagcgggt ttactcatta tgtcctcggc
    13081 aataaagggg gctggctatc aaactataga gcctttttgt cactgtcacg tgacgaatac
    13141 tcacgtaagt cgccgcgctt tctatacttg tggtgttcag atgattaaac aaccggagct
    13201 gcaatgatcg atctctattt cgccccgaca cccaatggtc acaaaattac gctgtttctc
    13261 gaagaagcag agcttgatta tcgcttgatt aaggtagact tggggaaagg cggtcagttt
    13321 cgtccggaat ttttgcgcat ttcgcccaac aacaaaattc cggcaattgt tgatcattct
    13381 cctgccgatg gcggcgaacc gttaagcctc tttgaggctg ctgccatttt gttgtatctg
    13441 gctgagaaaa caggactctt tttgagtcat gaaacgcgtg agcgcgccgc cacattacag
    13501 tggttattct ggcaggtagg cggactgggg ccgatgcttg ggcaaaatca tcattttaat
    13561 cacgcagccc cccaaaccat tccttacgct attgaacgtt atcaggttga aactcagcgt
    13621 ctttaccatg tactaaacaa gcggctggaa aactcgccct ggctgggagg cgagaactac
    13681 agcattgcgg atattgcctg ctggccgtgg gttaatgcct ggactcgcct gcgaattgac
    13741 ctcgcaatgt atccggcagt caagaactgg catgagcgga tccgttcgcg ccctgccacc
    13801 gggcaggcac tgctaaaagc acaactcggt gatgagcgtt cggatagtta acagaaacag
    13861 gttctcgtgt attatttcat cctaagtaaa acaacggaga acctgcaatg gcacaacctg
    13921 ccgctattat tcgtataaag aaccttcgtt tgcgtacgtt tatcggaatt aaggaagaag
    13981 aaattaacaa ccgtcaggat attgttatca atgtgacgat ccactacccc gccgataaag
    14041 cgcgcactag cgaagatatc aacgatgcgc tgaattatcg caccgtaacg aaaaacatta
    14101 ttcagcatgt agagaataac cgtttctctt tgctggaaaa attaactcag gatgtgctcg
    14161 atatcgcacg tgaacatcac tgggtgacgt atgctgaagt ggagatcgat aaactgcacg
    14221 cgctgcgcta cgccgattcg gtatccatga ccttaagctg gcagcgttaa tcgccatatc
    14281 gggaggctgc atgaacatag tgatcaccgg agggacggga ttaattggtc gccatttgat
    14341 tccgcgtttg ctggagctgg gccatcaaat tacggtagtg acgcgtaacc cgcagaaagc
    14401 cagttccgtt ctcggccctc gggtgacact atggcaaggg cttgccgatc aaagcaacct
    14461 caacggcgtt gatgcggtaa tcaacctggc cggagaaccg attgctgata aacgctggac
    14521 tcacaagcaa aaagagcgtc tctgccaaag ccgctggaat atcacgcaaa aactggtcga
    14581 tttgattaat gctagcgaca cgccgccgtc ggtactcatt tccggctcgg caacgggcta
    14641 ttatggcgac ttaggtgaag tggtggttac cgaagaggaa ccgccgcata acgaatttac
    14701 tcataaactc tgcgcccgct gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg
    14761 agtgtgcctg ctgcgaaccg gcgtggtact ggcaccggac ggcggtattc tcggtaaaat
    14821 gctgccaccg tttcgtcttg gcctgggcgg gccgattggt tccggtcggc agtatctggc
    14881 ctggattcat attgatgata tggtcaacgg cattctctgg ctgctggata acgagctgcg
    14941 cgggccattt aatatggttt cgccctaccc ggtacgcaat gaacaatttg cccatgcgct
    15001 cggtcatgcg ctgcatcgcc cggctatttt gcgcgtcccc acaaccgcca ttcggctgtt
    15061 aatgggcgaa tcttcagtac tggtattagg tggacaacgc gcgctgccta aacggctgga
    15121 agaagcgggt tttgcgtttc gctggtacga tttagaagag gcgctggcgg atgtcgttcg
    15181 ctgatgtggt ttacagcaaa catccgccag ttaactccct gtgttacagg attagtggct
    15241 ttgcgcgata agatcgtctg gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa
    15301 acctctatcc agcatctcct gagcaatacg cagggcttct tcgtgtttgc cctgcattgc
    15361 gccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttctt gtggtgcgcg
    15421 ttctgttatc tcaccaataa atgcacggaa acgctgggca tcccctgttt gtaatacgta
    15481 attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactaaca gcgaaacaat
    15541 ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag
    15601 cgccattttg cggtgttgca taatctcgtc atccggcacc acggtaatat ccaccaacgg
    15661 aaacgctgat gaatatattt tgcgggctat agcaggctcg gcaaattcat caagccagca
    15721 gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga tcaccaatgg
    15781 aagctcttta tagcccgcat caagatggtt ttgcattgcc gcaatggaat aacgcatcat
    15841 gcgaaaagcc attaattctt ccggcttact ttggtgctct atcactacat aaatataacc
    15901 cactccctcc tgcgttttca cagaccacaa gaggtcggaa taatattgcc gcaggtcttc
    15961 atcaataaaa ctgtttggtt ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg
    16021 cgcgggaaga tgaatattaa taaaattccg cgcggtgtct ggatggcgta aaaaagattt
    16081 aaataccgca tcatgcggcg tggaagttgt cgatattgtc atggcgttcc gtcacccctc
    16141 aaatttaaga tgacgcgaca ataaccacga ttaacaccca atgcagcagg cagtttttat
    16201 ctttggacag cgaattcaga agtattttta gcggactgca acttttacat ttcagcggaa
    16261 gctgactcgc aaattcccct tgaaaatact ccggcagcgt tagccaccgg agaggaaatt
    16321 atttcagcga tcccttaagg aactgttgca gacgagggct ttgcgggttg ccaaataact
    16381 gctccggcgc gccctcttct tctattttcc cctgatggag gaaaatgacg tgagtagaaa
    16441 catggcgagc aaagcccatt tcgtgagtca ctaccaccat ggttttcccc tcttctgcca
    16501 gttgctgcat aatacgtaac acttcgccta ccagttcagg atcgagcgcc gaggtaggtt
    16561 catcaaacag taaaacttct ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt
    16621 gctgaccgcc ggaaagatgc accggatatt tcccctgcgc acgttcgtct atcccgactt
    16681 tcgccagata cttcaccgcc cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa
    16741 tcggcgcttc catgacgttt tccaacaccg tcatatggct ccagagattg aagtgctgga
    16801 ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt tttatcggcg actttgagtt
    16861 gaccgtcttt gtcgcgcacc agattgatcg tctggccatt gaccacgatc gatccttcac
    16921 tcggtttttc gaggaagtta atgcagcgca gaaaggtact tttccccgat cccgacgatc
    16981 cgatgatgct tattacatct ccggcattcg cttgcagtga gacccctttc agcacttcgt
    17041 gttcgccata gcgtttgtgc aaatcgataa cgtttaattt attctcggac atcgtattct
    17101 cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt tttccgctct gcgaaagagg
    17161 ctgatcagga cataagagat gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa
    17221 ggttgatacg tggcggcgtt aatatcgcgg gctattttca gcagatccgg cacagtggcc
    17281 gtaaacgcca gcgcagtaga gtgcagcatc aggatcactt cgttgctgta tgccggtaac
    17341 gcaatacgca gcgcagaagg caaaataatg cagcgataca ttttaaaggt cgagaagcca
    17401 taggctctgg cggcttcaat ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc
    17461 tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca cggtacagtt caggccactg
    17521 cggaagaaag cgttaaggaa ttcggttccc ttaacaatct caagcgtgta catgccggaa
    17581 tagaacacca gcaactgaac atacagcggc gtaccgcgaa aaatataggt aaataaccag
    17641 attggaaact ggatgtattt attactggag acacgaccaa tcgccagaaa cagcgccagg
    17701 actccgccta tcactaccga caaaataagc agccacagag tgatcgccac accagtaaag
    17761 cgataaccgt cggtccacag cagcggtttc cagtattcat gtaagatttc gatcacaggt
    17821 cagccctctt cacacccacg gagtagcggc gctcaaggaa cagcagcaca ccattggaaa
    17881 cggtggtgaa aaccaggtaa atcacgccac agacgatggc gaaatagaac ggttcccagg
    17941 tacttttgcc tgccagctgc gtggctttga ccacatcttc caggccgagt aacgaaacca
    18001 gtgcggtaga tttgaggatc acctgccagt tgttgccaat gcctggcagg gcgtaacgca
    18061 tcatcgccgg aaacatgatc cgccgaaaca cttgcccgcg agtaaaaccg aacgccgtcg
    18121 ccgcctctat atgccctttc ggcactgcca taaacgcgcc acggaacgtt tcggtaaaat
    18181 aagcaccgta gataaaaccg agagtgataa taccagcgac catcggatcg atatcaatct
    18241 gcccgacgcc catcgcctcc gtcaccatgt ttagcgcaat ctgcaaacca tagaaaatca
    18301 gcagcatcaa caccaagtcc ggcacgcctc gaatcagcgt ggtgtaccct tcgaaaatca
    18361 gccccgaaag ccgattttgc gagagcttac cgccagcgcc aattaaaccg atgattacag
    18421 cgagcactac agagctgata gccagctcca gcgtgacgag cgcaccctgt aaaataacac
    18481 ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt
    18541 ctccctccgg ttacccgaag ggagcgggct ttctttcacc gatggcgatt aaccaccata
    18601 aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc
    18661 ggcaaaggct ttgttcagtg cctcgcgcag ttcgttatct tctttacgca ggcccatgcc
    18721 ggtccctacg ccaaacagtt tttcatcttt aacagacgga ccaccgaatt tgtaatcttt
    18781 accgacgggt tgtttgagga aaccttcgct ggcagcgacc tcatcctgga acgcggcatc
    18841 aatacgtccg gcagtcaggt cagaataaat gttgtcctgc ccctgatacg agacgatttc
    18901 aatgcctttt ggtgcccagt gttcattacc gaacgtctcc tgggtggtgc cctgcaatac
    18961 gcctacccgt ttgcctttca gcgactcgac tgtcggctga atgtcagaat ttttcgccac
    19021 caccagacga gaatcggcag cgtacagttt gtcggtgaag gcaatttctt gctgacgttt
    19081 ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc ttcgctttta aggacgggat
    19141 taacgcatcc agcgggtttt cgacaaacgt acattgcgta ttgatgcgct tgcacaactc
    19201 cttcgccagc tcgatatcga aaccaaccag ttcgccttgt gaattttttg attcaaatgg
    19261 cgcataggtc gggtcggtac cgatgcggat gttttgcgga attgcagcaa gtgccgcagt
    19321 tgcgctggag aaggccagaa ccagagagag cgatagcacc agttttttca taatgtcctc
    19381 aactgacagt ctttttatag ggaatattta caggtttgtt acacttatcg tgccataaaa
    19441 ttgaccatca aggcaaaata tcccgccccg ttagacgtat tatttttcat taaaaatgct
    19501 taagtatgca tttcaatgca ccacgatagt gcaacaaacc tggcgtgcac catgatgatc
    19561 attccatcag gtacagcttc ccagcgacgt atcagtcacc gtagacatta aagtcgaaat
    19621 actttttcgc catcttgtcg taggtgccgt cctgacgcag ctcgccaagc gccttattga
    19681 aggcagccgt cagttcagca taatctttac gtagccctac accggtgcca tcaccgaagt
    19741 attttttgtc ttttactgat gagccagcaa aagcgaaatc tttaccagca ggttgcttga
    19801 ggaatccttc gctggcagca acttcatctt gtaacgcagc atccagacgt cctgcagcca
    19861 gatcggaata gaccaaatcc tggttggcat aggccaccac atccacgcct ttactacgcc
    19921 aggtctcgtt agcgtaagct tcctgggttg atccctgcag cacaccaaca tgtttacctt
    19981 tcagtgaatc cagcgttggc tgaatcggtg aacctttggc agcaatcaaa cgagaatctg
    20041 cggcgtacag cttgtcggag aaggcaatct cctgctgacg tttatcggta atggaaagcg
    20101 acgaaataat agcgtcgatt tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac
    20161 tggcaaccca ggtacatttc acctgcatcc gtttgcacat ctcgttaccg agatcgatat
    20221 caaagccaac aaaatcacct ttagcatctt tcgacgagaa cagcgcatag gtggtatcgg
    20281 ttccgatacg taccgtctcc ggtagcgccg catagctgga agccgctgcg gagagaccga
    20341 ctaataaaga cagagcgaga atcgacttct tcatacataa ccctcaagtg aaaatggctt
    20401 ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt catgcaggtc ttatgccatc
    20461 ttgacgacaa cagtaacatt caacgttaaa tatgttaata agacattgca ttattgtcct
    20521 gaagttgaag atagcaggta tagcggttga atcgcagcgt ttcgattgaa tggcagaaac
    20581 aaaatgtcga ggatttgatc gcggttgcaa aattgccctg aaacagggca acagcggagt
    20641 tatgcgccct gccagcgggc aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga
    20701 acacgattaa ccgtctgatt tatcacatca tcaagggatt gcgggcgatg ataaaacgcc
    20761 ggaacgggag gcataatcac cgcaccgatt tctgccgcct gagtcattaa acgcagatgg
    20821 cctaagtgca atggtgtttc acgcacgcag agcaccaacg ggcgacgctc tttcagcacc
    20881 acatctgccg cacgggtcag taagccatca gtatagctat ggacaatgcc ggacagggtt
    20941 ttgattgaac agggtaaaat caccatcccc agcgtctgga aagaaccgga agagatgctg
    21001 gcggaaatat cgcgcgcatc gagcgtgaca tcagctaatg cctgcacttc gcgcagggaa
    21061 aaatccgttt cgagggataa ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg
    21121 atatctgtga catcgcgcag aacctgtaat aagcgcacgc cataaatcgc gccgctggca
    21181 ccgctgatgc ctacaatgag tcgtttcata aatagttgcc ctgtcagact tgcaggcaga
    21241 ctttgcagta tttcggcggg agttgcaagt cagggtgcca gaccggcacc ctcagcgaag
    21301 gcatcatcct tcgttatgca tttcgagatt ttccacttcg ttctgacgtt gcactgcttt
    21361 ggcgtcatca ttacgtaacg tatcgaggaa atcgaggtag ccctgatcaa catctttggt
    21421 gacgtagacg ccgttgaaca ccgagcattc aaactgctgg atatccggat tttcagcgcg
    21481 aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg
    21541 gcgaatttca tcaacttcgc gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat
    21601 accataaacg ttcgggaagc gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc
    21661 tccggcttcg cgtgccatct cgataatctg ctcagaagtg gtgccacgga cgatggagtc
    21721 gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg
    21781 cacggactta cgacgcagct gctggcccgg catgataaag gtgcggccaa catagcggtt
    21841 tttaacgaag ccctggcggt acggtttgcc cagaatacga gcaatttcca gcgcgatatc
    21901 acacgaggtt tctgggatcg ggatcaccac gtcgatatcc agatcttccc attcgcgggc
    21961 aattttctcg cccagtttcg tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc
    22021 gataaacgag tccgggcggg caaagtatac atactcaaac aggcacggat tgctgaccgg
    22081 attgtcagca cattgacggg taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc
    22141 cggcgcgacg tcacgcagga aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc
    22201 gaccatatat tctgtacggt tctcgtcaat atcacgtttt cccagtacca gcggacgaat
    22261 cccgtttgga tcgcggaaag caaccatacc gtggccgata atcatcgcca cacaggcata
    22321 cgcgccgcgg attaagcggt ttgtggcagc aatggcagcg aaaatattgt cggcttccag
    22381 cgggtagtgg cggaagttgt ccagctcgct ggcgaagata ttaagcagaa tttccgagtc
    22441 ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt tttttacgca actcgtgagc
    22501 gttggtcaga ttgccgttgt gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg
    22561 ctgcgcttca gaggcgctgg agctgccagc cgtagggtaa cgcacatgac caatgcccat
    22621 attgccctgc aaacgctgca tatggcgagc ttcaaataca tcgctcacca gcccgttcgc
    22681 tttacgcaaa cggaagcagt tattggcatc tatggtgatg atgccggcgg catcctgacc
    22741 gcgatgctga agcaccgtta aggcatcata aatcgactgg ttaaccggca taacaccggc
    22801 gataccgaca ataccgcaca tacgtctttt tcctcgttaa gccacatctc agagcactta
    22861 cgctctgggc aagaaacttg acgagctttg cagataatca aaaaaccatc tgatgataaa
    22921 actgaattgc gggatcagct gtgatttgct ccagtcttca cttttcgaca ccccggtaaa
    22981 ggagtcgaga aagaagagaa tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca
    23041 gacgcccagc acccgatcgg tgcctgacaa ccccgttttc tccaccaact ggcctatcac
    23101 gaagttcacg atagcaccaa cgatcagggt agcgataaac agtaccgcga tggcaatccc
    23161 atttcgaacc agttcgtctt caaagcccgt aaaccagact gacaggtaag tgtagtaatg
    23221 actggcaaca aagaaagcac aaccccatgt caccagcgat aacgcttcac gaacaaagcc
    23281 gtggatcagg ctaaccagag aggaaaaagc aatcaccgcg attatggcgt aatcaatcca
    23341 gaccatatgt gtcccacgat tttacgccct gtcatcctgt tcggggcgca ttctaacaga
    23401 aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc aataaaaatg gcgctgaaaa
    23461 aatattcaac gccatcgact ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc
    23521 ggcgtaaacg ccttatccga cctacggttc taacactgcc caagcctgat aagacgcgcc
    23581 agcgtcgcat caggcaagac tgtattaatt cggcgtatag cccattacca cgccacttaa
    23641 gccagaaagt tgcttcaact cacccagcga acctttcagc ttatctttcg aggcatccgg
    23701 cccaaccaga atacgggtaa ttttaccctg cactggcgtg gatggcgacg tataaacccg
    23761 ataaccggca ccgcgcagct taccgacaat ctcattcact ttatcggcat ttttcagcgc
    23821 acccagttgc acaacatagg ctttacccgt tggcgctgct ttttcttcca cgaccggctt
    23881 cggttctggc ttcggcgcag gtggtacttc aacctttggt ttaggcggct ccaccggttt
    23941 cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac tcggtgttat tggctgcaat
    24001 agtggccgga tcgagcgacg gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc
    24061 ttccggcggc tgcgtcggca acgcctgggt ggcggctggc atcatatcag gctcatcacg
    24121 atcgcccgct ttcggcacca gcgggatagc cgcgaactca tcctgataat gttttttctg
    24181 cccgtccagc agccctggaa gtacaatcac ccccagcgcc accagcacga tcgtgcccac
    24241 taaccgattc tgaaacttac ttgccaccgc ttctcctcgc gtcaatcact tccatgacat
    24301 gtgcgaccgt gtggaaagaa ccacacacca gcacggtgtc ttccgcttta gcgtccgcca
    24361 ttgcggcatc ccatgcctgc gcaacgctat caaatgattt gccgttaccc aaatgctcaa
    24421 gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa
    24481 ccacgctttt caaccaggcc agagttccgg caatatcttt atcatgtagc ataccgataa
    24541 ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg cccggtgaga tattccgccg
    24601 catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac
    24661 gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc attttcactg acttccagcc
    24721 cgctggcacg cagtgccgcc agcgccgttg cggcattcgg ttgcgggaca agcggcaacg
    24781 gcagattttc cagcgtgccg tgagcatcgc taaacgccca gtcatgatcg gtgacggaat
    24841 agttccactc aacgccccga cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa
    24901 tggtagaagg catttccggc tcaccgacaa ttgccggttt ttcgctgcgg aagatgcctg
    24961 ctttctcgag accaatactt tcgcgatctg gacccagcca gtcggtatga tccagcgcaa
    25021 tactggttac taccgcgaca tcggcatcga caatattggt tgcgtccaga cgaccgccca
    25081 gccctacttc cagaatcacc acgtcaagtt gtgcctgctt gaacagccac aacgccgaca
    25141 gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg tgccgattca atctccgcaa
    25201 aagaggcggt gtgggccgat tccggcaatt cctggccctg cacacgtacg cgctcggtat
    25261 aacgcaccag atgaggcgaa ctgtagacgc ccactttgta ccctgccgcc atcagaatcg
    25321 actccagcgt acggcaggtg gtgcctttgc cattcgtacc cgcaacggta aacacaaatg
    25381 gcgctggttt caggacgcca agacgcgccg cgacctggct cacgcgctca aggccgagat
    25441 cgatagtttt actgtgcagg ttttccagat aagaaagcca cgaagccaga ggcgacgcgg
    25501 cttgaggagt gcgtttgata atcatggtat ccgctgattc gttacggtga gaatagcaaa
    25561 agggcagagc cagtggccct gcccttatca gttatcaggc ctcaggttcc tgatccggta
    25621 ccgggggtac cactacgcct tcacgcggcg cttcaggatt cggcgctggc agattcatca
    25681 acttcgccag aatgctcgcc agtttcaggc gcatttccgg acgacggacg atcatgtcga
    25741 tcgcgccttt ctcgatcagg aattcactgc gctggaatct aggcggcagt ttttcgcgaa
    25801 cggtctgttc gataacacgc ggaccggcaa agccgattaa cgctttaggt tcagcgatgt
    25861 tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc catcgtcggg tcggtcagca
    25921 cggagatgta cggcaagccg cgctcctgca tttttgccag tgccgcagag gttttcgcca
    25981 tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc accagaggcg gagaagcaga
    26041 tcagcgggca gttatcttcc tgcgcctgct caacggcacg cacgaaacgt gcacccacaa
    26101 cagaccccat tgaaccgccc ataaaggcga actcgaatgc cgcagcgaca accggcattc
    26161 catacagagt gcctttcatc accaccagcg catctttttc gccggtttct ttctgcgcag
    26221 atgccagacg gtctttatac ttcttggagt cacgaaactt cagctcatct ttcggctcaa
    26281 gctcgctacc cagctccaca aggcttcctt catctaacag gctatgcagg cgattacgcg
    26341 ctgtcatacg catgtgatgg tcacacttcg gacagacctc aagattacgt tccagctcag
    26401 cgcggtataa aacctgaccg cagctatcac acttagtcca caccccttca ggaatgctcg
    26461 ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat ccagctcatt agggaccttt
    26521 ctgtctgaac ctggttcgat gccagtttta tctttgggga cgcataatgc catttttgcc
    26581 cccaacagac catgaatgtt gcacattaaa acataacagc ccgaaacttt ggataaaaaa
    26641 gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg
    26701 gattatttcg atgacgcccg gcaaaataga aaccacaata atcccgacga tcagcagctt
    26761 aaggttatcc tgaaccatcg ggattgtacc gaagaaatag cctgcgtagg taaaaagcag
    26821 tacccacaac agtgcgccga tcacgttata agcagcgaaa tgacggtacg acatgtggcc
    26881 cattcccgca acaaacgggg cgaacgttct gacgatcggc acaaaacggg cgagaataat
    26941 cgttttgccg ccatgtttct cataaaactg atgggttttg tcgagataac tgcgacggaa
    27001 aattttcgaa ttgggattac tgaataactt ttcaccgaac aaccgcccga tagtgtaatt
    27061 gaccgcgtca cccacaatcg cggcgatcag catcagcact accatcatat ggacgttgag
    27121 atcgttggtt tccagtgatg ccaacgctcc ggcaacaaac agcaatgaat cacccggtaa
    27181 aaacggcatt accaccagac cggtttcaca gaacaaaatt aagaacaaaa tggcataaac
    27241 ccagacgccg tactccgcga ctagctctgc cagatgcacg tcaatgtgca ggataaaatc
    27301 gatgaggaaa taaatcaggt ccatatttgc ctatgccttg tactcgtcat ctttcaggct
    27361 gtaactacgt tggctacagc ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa
    27421 atagcgggcc cattggcggt tttggaagat cataccggtc agggtaatcc accgcgacca
    27481 gatacagccc ttccgctttt gccgttgctg ccgccagcgt tctgtccttt gccgccagca
    27541 actctgctat ccagctctcc ggctggttgt gggcaccgac ttccatcagg ctgccgacaa
    27601 tattcctgac catatgatgt acaaaggcat tcgctttgat atcaaccacc acataaggac
    27661 cgtgacgcgt gacattaata tgcataacgt tgcgccacgg ggttcgggac tggcactgca
    27721 ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc
    27781 gttcagcgtc cagcggttcg taaaaatggg ttaccccttt actcagtacc gccgggcgca
    27841 gccgatgatt gtagatgatg tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa
    27901 aatcatcagg tacagcttta acccaacgca cggcgatgtc accaggtaaa ttcgcattta
    27961 cgcccaaggt ccacgccgcg tctttgcgct gcgcggttgt ttcgaaatgc accacttgcc
    28021 cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg
    28081 ccacctggga gagcgccttt tccagcttct cctgcacact gcggacttca ttctgccgtt
    28141 gccagccgta atacttactg ccgtcgtact caatgcccag cgcaatttta taaactggca
    28201 gttgttgctg gtcggacatt agtacagata ctcctgcacc agtttctcgg cgatttttac
    28261 tgccatcagc gcgccgccaa agcgaacgtt atcggccacc gaccagaact ggacttgctc
    28321 cggcataccg tagtcattac gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc
    28381 ccctacctga gtcgggaatt cgttctcttc agagagcaca atatcttcgc cctgagcaaa
    28441 cgcatcacgc gcttcttctg ccgccagcgg acgcagtgct tcaaagttga ccatctgggc
    28501 atgaccgtag aataccggtg cctggacgac gcttgccgaa atcatcagcc cttcgtcctg
    28561 caggattttg cgtacttcgt caacgatacg acgttcttca cgcacgctac cttcgctatc
    28621 cggcaacaac ggcagcatat tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat
    28681 cggaatgccg ttgagcaatt tcgcactctg ccccgctaac gcatcgaccg cttttttgcc
    28741 ctgggcggag gctgaaatca ggctggtaac actgatacgc gacaaaccgc cctgatcgat
    28801 taacggtttc agtgccgcca gcagctggct ggtcagactg tctggtacgg cgatgacatt
    28861 ccggttgcgg taatcggtca gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc
    28921 gggttcgaga gcaaacagtc cgctgctgtc gatcaccagg caacctgagt tggtcgcttc
    28981 ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt
    29041 ccagtcgaat tcagcggcat cctgcacggt gattgtttta ccaccaaagc gcagttgttc
    29101 gcctgcgctt tcgttacgtg ccagtgcata aatttcccca accgggaact gacgttcagc
    29161 cagcgtttca agcagggctt cgcccatagc gccagttgcg cccaggacgg caatgttcca
    29221 gccttcagac atggtggttt actccagaaa tagcaaagct ccctgccaat gttacagcag
    29281 agagcatgaa gaagagatta acgtgccgga tgatgaacgg cgttaaaacc cagtttacac
    29341 agcaatgatg ccgcactggc gtcatcacaa attacataca gagatgacca ttcacggcgc
    29401 tcaagatagt ttttgcgcag tttatcgaac tcacccggta tcccggcgac tttacgcagc
    29461 ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca gccttttcag cgtcggttga
    29521 tcgagcggac catgcagcgt aatgcggcca aactcaggcg cgggcagcaa cgtatccagc
    29581 gcaacgtgtt gttcatgccc aataaacttg ctataggctt caaacacttg cgtcgtgcca
    29641 cgcgctttac cctccagggt atagcctgcg atatgcggcg tgccgatatc cacttttgtc
    29701 agcaattcca cattaagttc cggctcgcct tcccagacat ccagcaccac gcttaacttc
    29761 tggccttcat tcaggcaggt cagcagcgcg gtattatcga cgactgcgcc acggcaggcg
    29821 ttaatcagaa tcgctccggg cttcaggcta cggatcagtt tttcatccgc cagatgtagc
    29881 gttttgtacg gaccatcttt aaagagtggc gtatggaaag tcagaatatc cgcgcgctgg
    29941 actaactcat ccagcgagcg gaaatcaccc tcatccccac ggtcggcgcg aggcggatcg
    30001 caaagtaagg ttttaatccc taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta
    30061 ccaacgccca cgatccccac cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc
    30121 atcagcaggg aggagaaaac atattccacc accgcaatcg cattacagcc aggtgcagcg
    30181 gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga catggtcggt ccccgcagtg
    30241 gcagttccaa caaatttaat gggttttcct gccagcaaag attcattcac tttcgtgacc
    30301 gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga cggggattgg acgcccggga
    30361 accgcgatca cctcacccaa acggctaaat aagtcgcggg cataaggcat attttcatca
    30421 acaaggattt tcacgtttgt gttacctgta tgagacgaga gttaaccgga caagtatgcc
    30481 ataatctcgc ggccaggcat acttgcgaag atttcaggta taaggatacg taatgataca
    30541 acctatttcc ggccctcctc ctgggcagcc accaggtcag ggagataacc tgccgtctgg
    30601 cgcgggcaat cagcctttat ccagtcagca acgtacttcg ctggaaagct taatgacgaa
    30661 agtgacctca ctgacgcaac aacaaagagc agaactgtgg gcgggtatca ggcacgatat
    30721 tggtctgtcg ggagattcac cgctgctttc gcgtcacttc cctgccgctg agcataatct
    30781 ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt
    30841 aggggagtat ttacgtctgg ggaatcatcg tcaggcggtc acggattata tccgtcataa
    30901 ctttggtcag acgccgctga atcagctctc accggagcaa ttaaaaacca ttctcaccct
    30961 gttgcaggaa gggaagatgg ttattccgca accacagcag cgcgaggcga ccgaccgtcc
    31021 tttattaccg gcggagcaca atgcgctaaa acagctggtg accaaacttg cggcggcaac
    31081 gggggaaccc agcaaacaga tctggcaatc gatgctggaa ctttccgggg tgaaagatgg
    31141 cgagttaatt ccagcgaaac tgtttaacca tctggtgacc tggctacagg cgcgtcagac
    31201 gctaagccag caaaatacgc cgacgctgga atcactacag atggcgctaa aacaaccttt
    31261 agatgccagt gaactggcgg cgttatcggc atatatccag caaaaatatg gcctttctgc
    31321 gcaatcatcg ctttcttctg cccaggccga ggatattctt aatcagcttt atcaacggcg
    31381 ggttaaaggg attgatccgc gtgatatgca accgctgctt aatccttttc caccgatgat
    31441 ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg atactgttag tcgcgattat
    31501 cctgatgctg gtctggctgg ttcgttaacc ccgacgaaat gacagtatcg tgacaataat
    31561 acccagcacc gcagagatcg ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc
    31621 cagcattccc gccagcggcc cggagacgcc gagggcgata tcctgaaacg cggcgtaacc
    31681 gcccagtgcg gtgccgcgaa cttgtgaggg gacgcgttta accacctcca cgcccagcgc
    31741 aggaaagata agcgaacatc cggctccggt taacgccgcg cccgctaatg cgacccatgc
    31801 acctggggct tgccagagca gcaacaagcc caccgtttct acaagcagag agacaatcgc
    31861 cactttcacg ccgccaaaac ggtccggcat ccagccaaac atgacgcgca tcacgacaaa
    31921 tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc catcctttgc tggcaaagta
    31981 gagcgaaacg aaagtcccga taaccgcaaa accaacgcct tgtagtgcca gccctaaccc
    32041 tggtttccag ataagcccga caacgctcca cagcgatgga cgttctcccg ccagggccgg
    32101 tactttgcgc actgtgccgt tacaggccca cgccagtaag ggtaatacca ttgtggtgat
    32161 cgccagtgcg gcaaaaccgt aatggctatg aatcaacagg ccaagcggcg caccaacagc
    32221 gagggcaccg taaatcgcca ttccgttcca tgacatcact ttgccagagt gttttggccc
    32281 tacgatgcct aacccccagg tcagagcgcc tgtcagtaac tggctttcac caaacccaag
    32341 aatcaaacgc ccgatgacca acagggcaaa tttgaacggt gcggagacag gcaaaatcgc
    32401 cgccagcagc aacgcgccgc cagccagacc acaagctaac atcccctgaa gcgccgaacg
    32461 ttttgcgcca tattgatcgg ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag
    32521 aaactgaatc ccgacggcaa tgccgaccat ggtattgcca tagcccagat catgatgaac
    32581 aaacagcggg ataaccggca acggcaaccc tacggtcatg taggtgagaa aaaccgcaaa
    32641 agagatgcgg aagagcgaaa aactggcaga agatcgtgtt tcggtttggc ttacagcagt
    32701 catgcattac tccagaatgc agcgcaaggc gaggagtatc cccgtctcat ctctctggtt
    32761 tcagggttac ggtgcgttgg caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca
    32821 aagcgggaag tttgcctaga actggtggcg attgtcaatg atgtgaaaaa gggaaccatc
    32881 aggttccctt ttgcgttagt gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct
    32941 ggcgcgtctt atccgaccta ctccggccta ctgcgaatta atcttttcag cttgcgcatt
    33001 accagcgtgg cgttggtgcc gccgaagccg aagctgttag acataacagt ggtcagttcg
    33061 cgatcggtcg tttcggtcac gatgttcaga cccgcagcct gctcgtccag ctcttcaatg
    33121 ttgatgctcg gggcgataaa gccgtgttcc agcatcagca gagagtagat agcttcctgc
    33181 acacccgcag cgcccagtga gtgaccggtc atggctttgg ttgcagaaat cgccgggctc
    33241 ttatcgccga acacttcacg gatagctgcc agctctttca cgtcgccaac cggagtcgaa
    33301 gtaccgtgag agttcaggta atcgattggg gtatcaacgc catgcatcgc catctgcata
    33361 caacgtactg cgccttcgcc agacggagca accatgtctg caccatcaga ggttgcgccg
    33421 tagccaacga tttcagcata gatgtaagca ccacgcgcca gcgcgtgttc cagctcttca
    33481 accactacca taccgccgcc gccagcgata acgaaaccgt cacggtgagc gtcgtaagta
    33541 cgggaggctt tttccggggt gtcgttgtat ttagtagata gcgcacccat cgcgtcgaat
    33601 tcgcaggcca tttcccagca cagctcttcg ccgccgccag caaacacgat gtcctgtttg
    33661 cccagttgga tctgctctac tgcgttaccg atacagtgtg cggaagtcgc acacgcggag
    33721 ctgatggagt agttaacgcc atgaatttta aacggcgtgg cgagacaggc agaaacgccg
    33781 gatgccatcg ctttagtgac cacatacggg ccaaccgctt tcaggccgcg cgggccgcgc
    33841 attgcgtcag cgccgaacac ctggaaacgc ggggaaccgc cgccggaacc tgcaatcagg
    33901 ccaacgcgcg ggttattctg gtaagcttcc ggagagaggc ccgcatcagc gattgcctgc
    33961 tccatagaaa ggaatgcata aatggatgcg tcgctcataa agcgcacaac tttgcggtca
    34021 atgaggccag tggtatccag ttttatgttg ccccagacgt ggctacgcat gccggaatcc
    34081 ttcagctcct gagagaaagt gatccctgaa cgtccttcac gcagagatgc caggacttcc
    34141 tgctggttat taccgatgct ggaaacaatg cccaggccag taatcactgc acgtttcatt
    34201 caatacctct gtaagtcgca catagagtaa gtttcgaatg cacaatagcg tacacttgta
    34261 cgccgaacaa gtccgatcag ccatttaata gagatatttg cgcagcctta cacacatcgc
    34321 taagatcgag ccaccgcctg taagacgagt aacttacgtg aaacactact ccatacaacc
    34381 tgccaacctc gaatttaatg ctgagggtac acctgtttcc cgagattttg acgatgtcta
    34441 tttttccaac gataacgggc tggaagagac gcgttatgtt tttctgggag gtaaccaatt
    34501 agaggtacgc tttcctgagc atccacatcc tctgtttgtg atagcagaga gcggcttcgg
    34561 caccggatta aacttcctga cgctatggaa ggcatttgat cagtttcgcg aagcgcatcc
    34621 gcaagcgcaa ttacaacgct tacatttcat tagttttgag aaatttcccc tcacccgtgc
    34681 ggatttagcc ttagcgcatc aacactggcc ggaactggct ccgtgggcag aacaacttca
    34741 ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta ttgctcgatg aaagccgcgt
    34801 gacgctggat ttatggtttg gcgatattaa cgaactgacc agccaactgg acgattcgct
    34861 aaatcaaaaa gtagatgcct ggtttctgga cggctttgcg ccagctaaaa acccggatat
    34921 gtggacgcaa aatctgttta acgccatggc aaggttggcg cgtccaggcg gcacgctggc
    34981 gacatttacg tctgccggtt ttgtccgccg cggtttgcag gaagccggat tcacgatgca
    35041 aaaacgtaag ggctttgggc gcaaacggga aatgctttgt ggggtgatgg aacagacatt
    35101 accactcccc tgctccacgc cgtggtttaa tcgtacgggc agcagcaaac gggaagtagc
    35161 gattatcggc ggtggtattg ccagcgcgtt gttgtcgctg gcgttattac ggcgcggctg
    35221 gcaggtagcg ctttattgcg cggatgaagc ccccgcgctg ggtgcttccg gcaatcgcca
    35281 gggggcgctg tatccgttat taagcaaaca cgatgaggcg ctaaaccgct ttttctctaa
    35341 tgcgtttact tttgctcgtc ggttttacga ccaattaccc gttaaatttg atcatgactg
    35401 gtgcggcgtc acgcagttag gctgggatga gaaaagccag cataaaatcg cacagatgtt
    35461 gtcaatggat ttacccgcag aactggctgt agccgttgag gcaaatgcgg ttgaacaaat
    35521 tacgggcgtt gcgacaaatt gcagcggcat tacttatccg caaggtggtt ggctgtgccc
    35581 agcagaactg acccgtaatg tgctggaact ggcgcaacag cagggtttgc agatttatta
    35641 tcaatatcag ttacagaatt tatcccgtaa ggatgactgt tggttgttga attttgcagg
    35701 agatcagcaa gcaacacaca gcgtagtggt actggcgaac gggcatcaaa tcagccgttt
    35761 cagccaaacg tcgactctcc cggtgtattc ggttgccggg caggtcagcc atattccgac
    35821 aacgccggaa ctggcagagc tgaagcaggt gctgtgctat gacggttatc tcacgccaca
    35881 aaatccggcg aatcaacatc attgtattgg tgccagttat catcgcggca gcgaagatac
    35941 ggcgtacagt gaggacgatc agcagcagaa tcgccagcgg ttgattgatt gtttcccgca
    36001 ggcacagtgg gcaaaagagg ttgatgtcag tgataaagag gcgcgctgcg gtgtgcgttg
    36061 tgccacccgc gatcatctgc caatggtagg caatgttccc gattatgagg caacactcgt
    36121 ggaatatgcg tcgttggcgg agcaaaaaga tgaggcggta agcgcgccgg tttttgacga
    36181 tctctttatg tttgcggctt taggttctcg cggtttgtgt tctgccccgc tgtgtgccga
    36241 gattctggcg gcgcagatga gcgacgaacc gattccgatg gatgccagta cgctggcggc
    36301 gttaaacccg aatcggttat gggtgcggaa attgttgaag ggtaaagcgg ttaaggcggg
    36361 gtaatctgct ctcgcagtgt ttgccggatg cggcgtgaac gccttatcca acctacgtgt
    36421 gacgtgtagg cctgataaga cgcgataagc gtcgcatcag gcatggtgct ccagacgccg
    36481 caacttacag ctgtgacgcc tgctgaaaca ggttttccca catatcggtc accagcgcct
    36541 ggtcacgcgg cgacaattca ccggcaccaa tggctttttc cagactctgg ctaacggtcg
    36601 tatgcaccgc ctgagcggag tggtcatcac cactttccag ttctgcgatg gctaatgtca
    36661 ggtggccacg caaataccca ctggcaaaca actcatcatc actggcatgg tcaaccatgc
    36721 cgtcgattaa tgccagaatg cgtgattcaa actccgcgat catcttcttt cctcattgta
    36781 aaacgtggcg ctggcttcag ttgagcgctt ccggccacgg gaactgttcc gccgtaagtt
    36841 ccggcgtgtg ataataattt tgtagtgctt taatgaagcg tgccgggcgt tccggaatgc
    36901 catttgtcag ataatccatc acctgcgcat gtacccgccg ttgaaacacc acacgatccg
    36961 gttcgaaatc cccttccaga ttgtcgcaac tgacgttaaa cggatatcca gccgccacgc
    37021 agaataacca gtcgaacgcc tgcggcttca cttcaacatc ttcaaactgg ctttgcgtct
    37081 gggcatcgcg tccgtccggg caataccagt agccgaaatc aaccagctca cggcgcgctt
    37141 ttccggcaat acaccagtgc gaaatctcat gaattgcgct ggcataaaag ccatgagcaa
    37201 agacgattcg gttatacggt acttccgcat cagcaggaag atagatcggt tcgtcgtcgc
    37261 ctttaatcag acgggtatta aattcatcgg caaagcagct attaaaaatt tcaatcaact
    37321 gctcgtagtg gtgtgtactg ttcattagtt catccccaac cagtggagga tctcctgtcc
    37381 gtggctatcg taaagtaatt tggcactcat caccgccgag acgataacaa tcatcgggcg
    37441 gatcagcttt tgtcctttgc tcaacaccaa ccgtgaaccc atgcgcgcgc cgaggaactg
    37501 ccccaccagc atgacaaagc ccgtcgccca aatcactttg ccgccgagaa taaacagcag
    37561 cagaccgcca atgtttgacg ttgcgttgag taatttggca tgagccgtgg ctttggcgag
    37621 gttgaatccg cacagcataa cgaaggccag cgcgtaaaac gatccggcag ccgggccaaa
    37681 gaatccgtca taaaaaccga cgcagccacc ggcaattaac gcgaagggta aaccgtacat
    37741 ccggcgctgg cgatcttctt cacccagctt tggcatcagc aagaaataga ggccgataca
    37801 aatcaccaga atgggcaaaa tctgccgtaa gacatcagcc tgaacgtact gtaccaacag
    37861 tgcgccgctc attgagccga caaaggtcat ggcaatattg agtttctgat cgcttaaact
    37921 aaccactttg cggcgaataa agtagatagt ggcggaaata gagccgccgc aggcttgcag
    37981 tttattggtt gccagcgcgt tagcgggaga cattcccgca gccatcaatg ccggaatggt
    38041 gagtaaccca ccgccaccgg caatcgagtc gataaatccc gccagcatgg cgacaaaaaa
    38101 gagaactccc agcaacagcg gagaaaccat aaacaggcta ttaaacgttt ccattagatc
    38161 acgtgctcat ccagtagcgc ctggcaggaa ggcggcaacg gaggcggtgt cttcttctca
    38221 ggctttgttg ttcccggttt tggaggttca aaccagcttt gcagttctgc cccgcaacca
    38281 tcgcctgatg gcggtaaagg ttgatcttca cactccagac tatcggcagg gcaacgtaat
    38341 cgtacatgca tatgcgcgcg atgctggaac cagggtcgca ctttgcgcaa ccagtcgcga
    38401 tcggtgcccg catcaaggca aagttgttgt ttaatcgccg gattaacaaa aatgcgcgtg
    38461 acgtctttgt cctgggcggc gagtttgatc aagctgaaaa tttctggctt ccacagcgtg
    38521 gagacaacgt gtttaccgtc gcgggaaact aagtccagtg cttgcgggcg caagagctgc
    38581 gcggaggtcc agcgagtttt cggcagttgc agaaagatat cgacatccag tccggtctgg
    38641 tggctggcat gaccgccgtt gaaacgccca ccagcgggca tccccatatc gccaatcagc
    38701 accgtaccca tgcccagatt gctcacctgg ctactcagac gctggataaa catcaccaga
    38761 tccgggtgac cgaaatagcg acgctgatcg gtacgcatga cctgataatg ttcggactgt
    38821 atcggcaacg tgtcagcgcc gacaatacag ccattagaaa aactgcctat cgattgtgcg
    38881 ctacccggca caggttgggt tattttttgc cacggcgtcg ctgccaggct agcactactg
    38941 gcaagcagag ccagcagcgc aatcgcggtt ttgttcattt tttaccagcg tggaatatca
    39001 gtcttcacat cggcattttg cgcccgctgc cgtaacaggt gatccattaa aacgatcgcc
    39061 agcatcgctt cagcgatcgg cactgcacgg atcccgacac agggatcgtg acggcctttg
    39121 gtgatcatct caacttcttc gccaaagcgg ttaatagtgc gccccggcac ggtaatgctg
    39181 gaggtcggtt tcagcgccat atgagcaatg atttgctgcc cgctgctgat accgccgaaa
    39241 atgccgcccg catggttgct ctggaaaccg tctttggtga tttcgtcgcg gttctggctg
    39301 ccgcgcagcg ccactacgtc aaaaccgtcg ccaatttcca cgcctttcac cgcgttgatg
    39361 ctcatcagcg catgggcgat gtcggcatcc aggcggtcaa agaccggctc gccaagtccg
    39421 gcggggacac cactggcaac aacggtgact ttcgcgccga tggagtcgcc ctcttttttc
    39481 agcgcgcgca tcagttcatc taacgcgtcg attttgtcgg ggtccgggca gaagaacgga
    39541 ttttgttcaa ccagcgacca gtctttgatt tccagcggaa tgtcgcccat ctgggtcagg
    39601 cagccgcgga tttcaatacc aaatttctcg gcgagatatt ttttggcaat cgcccctgcc
    39661 gccacgcgca tggcggtttc gcgggcggaa gaacgtccac cgccgcgata atcgcgcaga
    39721 ccgtattttt gttcgtaggt gtaatcagca tggcctggac ggaagacgtc tttaattgcg
    39781 ctgtaatcct gagaacgctg gtcggtattt tcgatcaaca agccaatgct ggtgccagta
    39841 gtaacgcctt caaaaacacc ggagagtatt ttgacctgat ccggctcgcg gcgctgggtg
    39901 gtatagcgcg atgtcccagg gcgacgacga tcaaggtcat gttgcaggtc cgcttccgtc
    39961 agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc gtgcgattcg
    40021 ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac ggctccgtta
    40081 ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatgttctc gtgcggcaat
    40141 aagctgctct ttagtgagca taaacacacc atcgccgccg ttatcaaact ccagccaggt
    40201 gaacggaaca tcgggatatt gttccataag atgtaccatg ctgttgccca cttcacaaat
    40261 caacacgcca tcatcagcaa ggtaatctgc cgcgttaccg agaatgcgac gcgtcagttt
    40321 caggccgtcg gtgccagatg ccaggcccag ttccggctcg tgacggtatt cgtttggcag
    40381 gtcggacata tcttccgcgt cgacatacgg cgggttagtg acaatcagat cgtattgcac
    40441 tttcggcaaa tcacggaaca gatcggaacg aatcggaatg acgttgtgga tcagaccgtg
    40501 ttcttcgatg ttctgttcgg caaccgccag cgcgtcggga gagatatcta ccgcgtcgac
    40561 ttccgcttcc gggaaggcat aagcacaggc aatggcgatg cagccgctac cggtacacat
    40621 atctaaaata tgctgcggtt gcttgctgat gagtccggca aatttattgt tgatcagttc
    40681 accaatcggc gagcgcggca ccagcacgcg ttcatcgacg taaaattcat gaccgcagaa
    40741 ccaggctttg ttggtcaggt aagccaccgg aatgcgctcg ttgacgcggc ggatcacgcg
    40801 ttcaacaata cggtgttttt cgctggaggt cagacgcgcg gtgcgcatat cttccgggat
    40861 atccagcggc aggtagagcg atggtaacac cagctgtacg gcttcatccc acgggttatc
    40921 ggtgccgtga ccgtaccaga tatttgccgc gctgaagcgg cttaccgacc agcgcaatat
    40981 gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat ccacgtattc
    41041 ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg acgataaatc
    41101 agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg tgctacgagt
    41161 cgggtaaact gtaggaaaat tagaaataga gacagataaa tgaaaaagaa aacaacactt
    41221 agcgaggagg accaggctct gtttcgccag ttgatggcgg ggactcgcaa gattaagcag
    41281 gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg aagtgccggt gaaacgcttg
    41341 atccaggagc aggctgatgc cagccattat ttctccgatg agtttcagcc gttattaaat
    41401 accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc attttgaggc gaagaaactg
    41461 cgccgcggcg attattcacc ggaattgttt ttggatttac acggtctgac gcagctgcag
    41521 gccaagcagg aactgggcgc gttgattgcc gcctgtcgcc gcgaacatgt gttttgtgcc
    41581 tgcgtgatgc atgggcacgg gaagcatatt ttgaagcaac aaacaccgct gtggctggca
    41641 caacatccgc atgtgatggc atttcaccag gcaccgaaag agtacggcgg tgatgcggct
    41701 ttgttggtgt tgattgaagt ggaagagtgg ttgccgccgg agttgccctg aatgctggca
    41761 gcaaatgccg gagcagcgta aacgccttat ccggcctaca aatatgtgat aattcaataa
    41821 attgcaattc acttcgtagg cctgataaga cgcatcagag caatactacg aacttcagtt
    41881 cattcaatac atactgaaaa cgacacgtcg aatcaatcgg ttgcccacta ctggtattca
    41941 cattcaccac aatatcgtgt agtactttat ccgttaccgg atgacgagtt ggcgtaaccg
    42001 ttaccgttga tttatcacaa ccgtaagagg taaaggttaa caattctgat accgcgcggc
    42061 agtatgctcc tctgccatca ttatcagaaa aattactgcc atcacagagt ctgccgcctt
    42121 ccatcaggta ggtcgaaagc gtggtggtca ctgttgaccc cgtggactga attcgtaacg
    42181 ccttattatg gctgacataa ttttcactcc aggagatatc gtatgaagcg accggtatcg
    42241 ttgggcatat tgtgttatgc actgtagggg ccgtatagct tccaaatccg ggtaaatctg
    42301 tataggaata aaaggatgcg atccaagggc aaccataatc atcagatgca acggtaattt
    42361 taaacgtcat atatgcacaa cgaaaaattt tcgtatctat gccagggcaa tttgagttat
    42421 taattgtatc aaagtcagtc agtaccaaag ttggagctga acccgaccca gaccaggaaa
    42481 taacccgcaa cgtatcgcct ttatacgcaa aagaatcata tttcccatat gcaacctcct
    42541 ttggcgttat caccgtaaca ttgaaaacac tgctcgctac agcaccggtt accttgtcca
    42601 tggcataata aactctcaca ctgtcaaaac tcgcactcat aagtttatct ccagcataac
    42661 tcttagatgc gccgacaaca ctcagaacga gcagcattag gctcgcgaaa attatggtcc
    42721 attttttcat ttcagaaata ctcaaaggtt gccgtcactg tggcactaaa gtctccggac
    42781 gttacatctc gcccgctctt tgcctgtaac caggtattaa aatttaggct gttatttccc
    42841 tgattcagcg ggagcgtgac gcctgttgta ttgttgattg gcaccgatgt gcctgccgca
    42901 gtttcaatac caatcccagc gccggatgct gatgacgagg agtcaaaggc cagaaagccg
    42961 ggtagagcac tatcttctgt tcctgtcaac gttactttga cattaaacaa tgtagaacta
    43021 tgacaatctt tcaattgaaa caccaatggc actcgctctg actgcccaag gttcattaaa
    43081 tcactggcgg caatggcagg aaaatgtact tctgccaact ggctaccatt aatcactggc
    43141 gtacacgatt tactgattaa attgccggta aagtgcaaat tatctcctgc tattgtggtt
    43201 ccactgccca gcaacatcag gctactaata aaaaacaacg tacgcttttt cttcattggt
    43261 aatctacctg taacgtggcc caggcctcaa agtccgcctc tggcagaatg acaccactct
    43321 tttttactgg cacagccgtt aacaccggta aatcggtttc attcaccaca agtggcgtat
    43381 tgatcgtaaa actctgccct gcctgtttga gctgaatgcc taaaccagtg atattgctgc
    43441 ttactgcagc attatcaaaa tcgcttggtg tgccactcca gctcaaggtc atttccatta
    43501 atgggtcgcg aaccgccgta tcgcaggtaa tggaatacgg cacggtttgc tcataattgc
    43561 tgccattaat gtcatcaata agcacatcct gaaaagttat ttcaatggtt tgagcgttgc
    43621 taatcgtgca gttaggcggc tgcactaaat aaccgtgaaa agagacgttt gagagtgcca
    43681 tcgagcaaaa gccccacagc aaaataagcg agatccgttt cattggtaat ccaccacaat
    43741 tgtggcactg gcgttaaatt cagctgccgc taacgttgta ccactctgtt tcaccggcac
    43801 agcttccaga acgggaactc ccgaggagaa attaaatggc aaccatgagc cagaggttaa
    43861 gtcgagtatt gtgtgatcac tacttttctg aacccgaata ccaaacccag gaatacccgt
    43921 gctgataact gtttcaccgt taatcacgac cgttgtcgcc tgcatctgta gacgcaggtc
    43981 atccattgcc agattgttac aaacaagcga gtagtcaatg ggacgtttat aatcaaccct
    44041 attgatctta ttaatgaata cattaccaaa ctcaactgcc gcaccattta ccgtacaagg
    44101 aggaggggca tcgacaagca cccgtaaatt catacttact gacttactcg ctgcaactgc
    44161 aaagccgcta ccaacacaac atatcagtaa cccagttacc gcccagacag aacaagatcc
    44221 tttcatactg gctctcccgg cctcagcctt tgcggctctg ctcttcatcg acctagcaac
    44281 tgttaccgtt gcaagtgaaa aagagcggca tccgagcgcc atagtcgttg acataggtca
    44341 gtaccggcac gctacccatc ttcacattca gcggtgcaga ggttttcggc gacatcacta
    44401 ttggcgagaa accggctgcc ggattgccat ttttctgtgt gctggcatta ctgaaaatga
    44461 cgtaatacga cgtcgggtta ttgacggtaa attcctgacc gttgcgggtt aaagtaatct
    44521 tatattgcta ggggtttttc atgctgacgt tctccagcgc cttcggtcgc cagaacaact
    44581 taatacgcgt ttgtagtgct atttgcagcg tgttggcttt gtttgatttt ggcgggatct
    44641 cacgcacgtt gaagtagaac aggctttcac gatcggcagg caatttgttg atatccggca
    44701 ttccctgcac ttttacctgc ccgttcatca ttgaatcgat gcgttgcacc ggtggcagaa
    44761 cagttaacgg tgaactgatc ttgttaccct tttcatcttc gatccagctt tgggccagat
    44821 aaggcagttt cggatcgtta ttgcgcaacg tcacgctgat tgatttgtcg ctttcattaa
    44881 atatcaaacg ggtacgatct ggcgttacag aagcctgggc agacaaactg gcagcgctaa
    44941 gcagtaacgc tagcgtcgta gcacccaatt tggcagagca aagtaaatca gacatggatc
    45001 attccttaat cacgttattg gttagctgaa acagaaacag ggggattggt gggtgtcacc
    45061 tgttgcgtct gctcctgaat gacaggcttt atgtcatcag gaacaaccgg cgcgacgttg
    45121 cctttatgct ggcatggcag taacaagccg ttaaacagat cggcaggcag cgggtccggc
    45181 aggttgatat cgcaatgcgc aacaccactc cagaacacac tcatgtgttc gccaggtttc
    45241 actccagcca gataaacatt gccgtcatca tcgacaaggc ccactgtctg ctcgttatca
    45301 ttttttactt ctgcgccaaa cggaggatgg ctgccgtctt gtaaacgcag caccgccatc
    45361 gctttttgac cactgatgac ggcaaatttg cggtagccaa tcgccccttc agttagcgtg
    45421 gcttgtacca ccgactgggt tgcttcggcg ttttccggca gtttgttgag gtcgatatac
    45481 gcctgattgc ggtaatagtt attgacgtca gaaacgacgg ctttaccaaa catattggta
    45541 taaacagcag caccgttacc ttctaccgga acatcggcaa cgccatcggc atcaatcaac
    45601 aagcgtgtcc cgcccatatt ctgggtacgg tgaagtgcgc caccgtgggc agtaagcgtt
    45661 gcgccgccct gtaacgacaa gcccgcagag gtgtattgcc cttcatggta gttcgcactg
    45721 aggtcaacct gcgccagcga accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg
    45781 tcactggtgc cgacgttcaa ctgatagtga gtcgcgtcat cgacacggct gaaataaccg
    45841 acctgactgc tgtccgtccc actgccatag taccgttata gctaacggta ctgttgtcgc
    45901 cccacggcat actgagcgaa atgtacatgc ctttgtcggc ctggttgtca tactcgtaac
    45961 ggtagccagt catcgagatg ctgacattac gaatactgcc catattgaaa tagtgcgaga
    46021 gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta ggtatggcgg gtatagttga
    46081 gataaaccga aacacccgca tcgcggaagt tctggttata agtggcggtg tacatctctt
    46141 tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc cagatactcg ctcatggtca
    46201 taaagttctc ttccgagaag cgatatccag caaaagtaac gcggctgttg agctggtcaa
    46261 agtctttgga ataactcaca cggaaggagt taccgtccag cgaacctttg ccataagcgg
    46321 tgtctttatc cagtttggtg tgcgagtgag taacatcaaa cgcgaccgcg ccgaatgtag
    46381 acaaatcgcg accgacacca agcgccgcag actgatagtt ttcatctccc agtgcgccgc
    46441 catataacga ccagccgtta gcgatccccc aggaagcttc agcaccagaa aaaaattcac
    46501 cctcgacatg gtgtccccac tcttgcggac ggcccatcat gatcttatag cgaacctgac
    46561 ctggacgagt gaggtatggc atcgaggcgg tgctgatgtc atattcctgc acctggccgt
    46621 tctgttcttc aatgcgaata tgcaacgtac cggagacaga atcaccaaga tcctgaatac
    46681 gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg ccccatctgg ctgacggtca
    46741 cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc gtagccgcgc agattgggag
    46801 gcaacatttg atcgtcagta ctgacactgc caccaacata gttaaaacca tcaaaaatat
    46861 cggatctgag gtaatcctcg cccagcgcca gtttggcttt taatgatggt aacgcccgcc
    46921 tggcatagta gcgactccac tcccattttt ttgagtttca tcgccgctga attcatcgtc
    46981 atcattactg cgagtatgtt gatagttagt ctgccagtca gcacgcatac gccacggccc
    47041 taggttaacc ccaaccgtcc cgttgccgct gatctcgtta ctatcatcac cgccattttc
    47101 ttcgtgccgg gtttgtgcgt tgatgctgta gtccgcgacg atcccggaga tgccgtcatc
    47161 ccaacgtgaa ggcggatccc tgtcgggata agtatattcg aggtaagcct gtggcagtga
    47221 aatgactaat gcggactggc ttaaatcagc cttaatttcc atgccttcca gttgaccgga
    47281 tttcaggcat ttagcatcgt ggctccattg cagatttttc gccacgtctt cttttaaacc
    47341 aaactgcgct accagttccg gtgtcagaca agcatagctt ttgctcgcgt catcttcacc
    47401 agcataccag taaatatcgt actcttccgc caatggctgt ttatttagtt gaacctgtaa
    47461 attgtatttt ccgggctcaa catatccctg gctggaaaaa cgcttcatat caatttttgt
    47521 gtcgcctttt aattccagaa aacgggaatc aaactgaatg tcgtcttcag cccagacact
    47581 gctataactc cctgacattg ccagcgcaat gcaccacgga agaatccgta atcgaaaaag
    47641 agaatggtca ggcatacacg tttctatccg tttttattct tgttcggtca aaatacattt
    47701 aaatccccca tagcagtgtc gctatggggg tatgccttac aggtaagtaa tctggaaggt
    47761 ggtatttgct tcaaaattac ccagatctgg cgcatcagct gcgccgacca gccaggcgtt
    47821 aaagttcagt gtctgcttcg ctttgccttt gttggtagaa gtgtctttca ctattttctg
    47881 gtcaataccc atgccgcttt tgtaagaggt acccagagag tcaccgatcg ccaggctaac
    47941 attgttaaat gccgcaccgg tatcggtatt gaaaatggtg taataattgc cgctatttgc
    48001 agaagaaacg gtaccagtaa aggtagtggt cattgtttcc tgagtatcaa atacgcaatc
    48061 ttgcagacga atctggaaac ttttcggcgt ggtggtgccg ttatttttca gtttttcggc
    48121 accgatatca cccatatcaa cttccagttt gtgatcgtcc ggaacgatag aacacactga
    48181 gtcttcaatg gtgccgtaaa aacgtgcagt accgttgtta cccgcagcga ttgttgccgt
    48241 agaggtgaac acgcccatca ccattgccgc agcaatagct gttttaacaa acttactcat
    48301 tatttgtcct taaactataa ataattaaaa atataaacat tgcaatacat tgattcagtc
    48361 aatagccaat gtttataccc agccattgaa tacggcatgc gacccaaaaa gaacaggtcg
    48421 tagaaaattg tattcattgg tgtggggccg catagtgatg tttccacacc cctgatccaa
    48481 tttaatttta ctaaagagta aatgtagatt taattaatat attgacagaa gaaaggttat
    48541 cttaagga
//
LOCUS       DAZPFP010000014        48321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPFP010000014 DAZPFP010000000
VERSION     DAZPFP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.014"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACHUR4_000728"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACHUR4_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1454431.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHUR4_000729"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHUR4_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR1454432.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="ACHUR4_000730"
     CDS             complement(1922..2374)
                     /locus_tag="ACHUR4_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1454433.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHUR4_000731"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHUR4_000731"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1454434.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="ACHUR4_000732"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="ACHUR4_000732"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1454435.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHUR4_000733"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHUR4_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1454436.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHUR4_000734"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHUR4_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1454437.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHUR4_000735"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHUR4_000735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1454438.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHUR4_000736"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHUR4_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1454439.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHUR4_000737"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHUR4_000737"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1454440.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHUR4_000738"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHUR4_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1454441.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHUR4_000739"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHUR4_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1454442.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="ACHUR4_000740"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="ACHUR4_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1454443.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHUR4_000741"
     CDS             complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHUR4_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1454444.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="ACHUR4_000742"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="ACHUR4_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1454445.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="ACHUR4_000743"
     CDS             complement(15085..15582)
                     /locus_tag="ACHUR4_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1454446.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="ACHUR4_000744"
     CDS             complement(15579..15941)
                     /locus_tag="ACHUR4_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1454447.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="ACHUR4_000745"
     CDS             complement(15931..16278)
                     /locus_tag="ACHUR4_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1454448.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /gene="yidI"
                     /locus_tag="ACHUR4_000746"
     CDS             16387..16800
                     /gene="yidI"
                     /locus_tag="ACHUR4_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1454449.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="ACHUR4_000747"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="ACHUR4_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="ACHUR4_000748"
     CDS             complement(18324..20039)
                     /locus_tag="ACHUR4_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR1454450.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="ACHUR4_000749"
     CDS             20206..21099
                     /locus_tag="ACHUR4_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1454451.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="ACHUR4_000750"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="ACHUR4_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="ACHUR4_000751"
     CDS             complement(21910..23526)
                     /locus_tag="ACHUR4_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1454452.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="ACHUR4_000752"
     CDS             23821..24495
                     /locus_tag="ACHUR4_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1454453.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="ACHUR4_000753"
     CDS             complement(24534..26195)
                     /locus_tag="ACHUR4_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1454454.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHUR4_000754"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHUR4_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1454455.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHUR4_000755"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHUR4_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1454456.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="ACHUR4_000756"
     CDS             27649..27981
                     /locus_tag="ACHUR4_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1454457.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="ACHUR4_000757"
     CDS             complement(27983..29215)
                     /locus_tag="ACHUR4_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1454458.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHUR4_000758"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHUR4_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1454459.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="ACHUR4_000759"
     CDS             complement(30377..31714)
                     /locus_tag="ACHUR4_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454460.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHUR4_000760"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHUR4_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHUR4_000761"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHUR4_000761"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1454461.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHUR4_000762"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHUR4_000762"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1454462.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHUR4_000763"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHUR4_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1454463.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /gene="yidX"
                     /locus_tag="ACHUR4_000764"
     CDS             35377..36033
                     /gene="yidX"
                     /locus_tag="ACHUR4_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR1454464.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHUR4_000765"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHUR4_000765"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1454465.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="ACHUR4_000766"
     CDS             complement(37005..37403)
                     /locus_tag="ACHUR4_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1454466.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHUR4_000767"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHUR4_000767"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1454467.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHUR4_000768"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHUR4_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1454468.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHUR4_000769"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHUR4_000769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1454469.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHUR4_000770"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHUR4_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1454470.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="ACHUR4_000771"
     CDS             43964..44044
                     /locus_tag="ACHUR4_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454471.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHUR4_000772"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHUR4_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1454472.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHUR4_000773"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHUR4_000773"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1454473.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="ACHUR4_000774"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="ACHUR4_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1454474.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="ACHUR4_000775"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="ACHUR4_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1454475.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHUR4_000776"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHUR4_000776"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1454476.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAZPFP010000015        46524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPFP010000015 DAZPFP010000000
VERSION     DAZPFP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.015"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            362..487
                     /gene="timP"
                     /locus_tag="ACHUR4_000777"
     CDS             362..487
                     /gene="timP"
                     /locus_tag="ACHUR4_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1454477.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(519..1295)
                     /gene="sseB"
                     /locus_tag="ACHUR4_000778"
     CDS             complement(519..1295)
                     /gene="sseB"
                     /locus_tag="ACHUR4_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1454478.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1437..2720)
                     /gene="pepB"
                     /locus_tag="ACHUR4_000779"
     CDS             complement(1437..2720)
                     /gene="pepB"
                     /locus_tag="ACHUR4_000779"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1454479.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2779..2979)
                     /gene="iscX"
                     /locus_tag="ACHUR4_000780"
     CDS             complement(2779..2979)
                     /gene="iscX"
                     /locus_tag="ACHUR4_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1454480.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2991..3326)
                     /gene="fdx"
                     /locus_tag="ACHUR4_000781"
     CDS             complement(2991..3326)
                     /gene="fdx"
                     /locus_tag="ACHUR4_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1454481.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3328..5178)
                     /gene="hscA"
                     /locus_tag="ACHUR4_000782"
     CDS             complement(3328..5178)
                     /gene="hscA"
                     /locus_tag="ACHUR4_000782"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1454482.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5195..5710)
                     /gene="hscB"
                     /locus_tag="ACHUR4_000783"
     CDS             complement(5195..5710)
                     /gene="hscB"
                     /locus_tag="ACHUR4_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1454483.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5806..6129)
                     /gene="iscA"
                     /locus_tag="ACHUR4_000784"
     CDS             complement(5806..6129)
                     /gene="iscA"
                     /locus_tag="ACHUR4_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1454484.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6146..6532)
                     /gene="iscU"
                     /locus_tag="ACHUR4_000785"
     CDS             complement(6146..6532)
                     /gene="iscU"
                     /locus_tag="ACHUR4_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1454485.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6560..7774)
                     /gene="iscS"
                     /locus_tag="ACHUR4_000786"
     CDS             complement(6560..7774)
                     /gene="iscS"
                     /locus_tag="ACHUR4_000786"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1454486.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7886..8374)
                     /gene="iscR"
                     /locus_tag="ACHUR4_000787"
     CDS             complement(7886..8374)
                     /gene="iscR"
                     /locus_tag="ACHUR4_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1454487.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8644..9384)
                     /gene="trmJ"
                     /locus_tag="ACHUR4_000788"
     CDS             complement(8644..9384)
                     /gene="trmJ"
                     /locus_tag="ACHUR4_000788"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1454488.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9503..10306
                     /gene="suhB"
                     /locus_tag="ACHUR4_000789"
     CDS             9503..10306
                     /gene="suhB"
                     /locus_tag="ACHUR4_000789"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1454489.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10451..11305
                     /locus_tag="ACHUR4_000790"
     CDS             10451..11305
                     /locus_tag="ACHUR4_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1454490.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11496..12776
                     /gene="csiE"
                     /locus_tag="ACHUR4_000791"
     CDS             11496..12776
                     /gene="csiE"
                     /locus_tag="ACHUR4_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1454491.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12768..13907)
                     /locus_tag="ACHUR4_000792"
     CDS             complement(12768..13907)
                     /locus_tag="ACHUR4_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1454492.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14067..14957)
                     /gene="hcaR"
                     /locus_tag="ACHUR4_000793"
     CDS             complement(14067..14957)
                     /gene="hcaR"
                     /locus_tag="ACHUR4_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1454493.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15093..16454
                     /gene="hcaE"
                     /locus_tag="ACHUR4_000794"
     CDS             15093..16454
                     /gene="hcaE"
                     /locus_tag="ACHUR4_000794"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1454494.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16451..16969
                     /gene="hcaF"
                     /locus_tag="ACHUR4_000795"
     CDS             16451..16969
                     /gene="hcaF"
                     /locus_tag="ACHUR4_000795"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1454495.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16969..17289
                     /locus_tag="ACHUR4_000796"
     CDS             16969..17289
                     /locus_tag="ACHUR4_000796"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1454496.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17286..18098
                     /gene="hcaB"
                     /locus_tag="ACHUR4_000797"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17286..18098
                     /gene="hcaB"
                     /locus_tag="ACHUR4_000797"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1454497.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18108..19325
                     /gene="hcaD"
                     /locus_tag="ACHUR4_000798"
     CDS             18108..19325
                     /gene="hcaD"
                     /locus_tag="ACHUR4_000798"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1454498.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            19422..19844
                     /locus_tag="ACHUR4_000799"
     CDS             19422..19844
                     /locus_tag="ACHUR4_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1454499.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19892..20764)
                     /locus_tag="ACHUR4_000800"
     CDS             complement(19892..20764)
                     /locus_tag="ACHUR4_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1454500.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20776..21870)
                     /locus_tag="ACHUR4_000801"
     CDS             complement(20776..21870)
                     /locus_tag="ACHUR4_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1454501.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21903..22901)
                     /locus_tag="ACHUR4_000802"
     CDS             complement(21903..22901)
                     /locus_tag="ACHUR4_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454502.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22926..24437)
                     /locus_tag="ACHUR4_000803"
     CDS             complement(22926..24437)
                     /locus_tag="ACHUR4_000803"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1454503.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24460..25442)
                     /locus_tag="ACHUR4_000804"
                     /pseudo
     CDS             complement(24460..25442)
                     /locus_tag="ACHUR4_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25539..28820)
                     /locus_tag="ACHUR4_000805"
                     /pseudo
     CDS             complement(25539..28820)
                     /locus_tag="ACHUR4_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28938..30131
                     /locus_tag="ACHUR4_000806"
     CDS             28938..30131
                     /locus_tag="ACHUR4_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR1454504.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30329..31582)
                     /gene="glyA"
                     /locus_tag="ACHUR4_000807"
     CDS             complement(30329..31582)
                     /gene="glyA"
                     /locus_tag="ACHUR4_000807"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1454505.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31909..33099
                     /gene="hmpA"
                     /locus_tag="ACHUR4_000808"
     CDS             31909..33099
                     /gene="hmpA"
                     /locus_tag="ACHUR4_000808"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1454506.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33144..33482)
                     /gene="glnB"
                     /locus_tag="ACHUR4_000809"
     CDS             complement(33144..33482)
                     /gene="glnB"
                     /locus_tag="ACHUR4_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1454507.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33543..34877)
                     /gene="glrR"
                     /locus_tag="ACHUR4_000810"
     CDS             complement(33543..34877)
                     /gene="glrR"
                     /locus_tag="ACHUR4_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1454508.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34867..35580)
                     /gene="qseG"
                     /locus_tag="ACHUR4_000811"
     CDS             complement(34867..35580)
                     /gene="qseG"
                     /locus_tag="ACHUR4_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1454509.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35745..37172)
                     /gene="qseE"
                     /locus_tag="ACHUR4_000812"
     CDS             complement(35745..37172)
                     /gene="qseE"
                     /locus_tag="ACHUR4_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1454510.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37730..41617)
                     /gene="purL"
                     /locus_tag="ACHUR4_000813"
                     /gene_synonym="purI"
     CDS             complement(37730..41617)
                     /gene="purL"
                     /locus_tag="ACHUR4_000813"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1454511.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41876..43432
                     /gene="mltF"
                     /locus_tag="ACHUR4_000814"
     CDS             41876..43432
                     /gene="mltF"
                     /locus_tag="ACHUR4_000814"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1454512.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43429..43932)
                     /gene="tadA"
                     /locus_tag="ACHUR4_000815"
     CDS             complement(43429..43932)
                     /gene="tadA"
                     /locus_tag="ACHUR4_000815"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1454513.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43990..44625)
                     /gene="yfhb"
                     /locus_tag="ACHUR4_000816"
     CDS             complement(43990..44625)
                     /gene="yfhb"
                     /locus_tag="ACHUR4_000816"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1454514.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44834..45682
                     /locus_tag="ACHUR4_000817"
     CDS             44834..45682
                     /locus_tag="ACHUR4_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1454515.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46021..46524)
                     /locus_tag="ACHUR4_000818"
                     /pseudo
     CDS             complement(46021..46524)
                     /locus_tag="ACHUR4_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11066 a        12793 c        11696 g        10969 t
ORIGIN      
        1 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
       61 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      121 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      181 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
      241 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
      301 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
      361 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
      421 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
      481 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
      541 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
      601 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
      661 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
      721 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
      781 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
      841 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
      901 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
      961 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1021 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1081 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1141 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1201 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     1261 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     1321 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     1381 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     1441 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     1501 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     1561 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     1621 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     1681 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     1741 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     1801 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     1861 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     1921 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     1981 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2041 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2101 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2161 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     2221 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     2281 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     2341 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     2401 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     2461 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     2521 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     2581 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     2641 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     2701 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     2761 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     2821 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     2881 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     2941 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3001 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3061 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3121 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3181 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     3241 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     3301 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     3361 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     3421 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     3481 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     3541 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     3601 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     3661 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     3721 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     3781 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     3841 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     3901 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     3961 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4021 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4081 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4141 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4201 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     4261 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     4321 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     4381 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     4441 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     4501 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     4561 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     4621 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     4681 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     4741 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     4801 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     4861 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     4921 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     4981 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5041 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5101 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5161 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     5221 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     5281 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     5341 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     5401 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     5461 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     5521 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     5581 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     5641 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     5701 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     5761 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     5821 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     5881 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     5941 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6001 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6061 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6121 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6181 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     6241 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     6301 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     6361 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     6421 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     6481 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     6541 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     6601 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     6661 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     6721 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     6781 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     6841 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     6901 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     6961 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7021 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7081 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7141 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7201 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     7261 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     7321 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     7381 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     7441 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     7501 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     7561 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     7621 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     7681 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     7741 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     7801 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     7861 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     7921 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     7981 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8041 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8101 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8161 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     8221 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     8281 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     8341 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     8401 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     8461 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     8521 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     8581 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     8641 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     8701 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     8761 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     8821 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     8881 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     8941 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9001 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9061 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9121 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9181 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
     9241 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
     9301 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
     9361 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
     9421 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
     9481 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
     9541 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
     9601 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
     9661 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
     9721 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
     9781 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
     9841 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
     9901 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
     9961 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10021 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10081 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10141 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10201 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    10261 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    10321 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    10381 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    10441 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    10501 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    10561 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    10621 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    10681 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    10741 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    10801 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    10861 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    10921 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    10981 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11041 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11101 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11161 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    11221 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    11281 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    11341 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    11401 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    11461 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    11521 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    11581 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    11641 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    11701 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    11761 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    11821 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    11881 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    11941 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12001 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12061 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12121 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12181 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    12241 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    12301 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    12361 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    12421 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    12481 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    12541 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    12601 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    12661 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    12721 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    12781 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    12841 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    12901 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    12961 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13021 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13081 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13141 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13201 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    13261 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    13321 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    13381 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    13441 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    13501 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    13561 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    13621 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    13681 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    13741 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    13801 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    13861 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    13921 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    13981 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14041 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14101 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14161 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    14221 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    14281 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    14341 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    14401 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    14461 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    14521 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    14581 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    14641 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    14701 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    14761 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    14821 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    14881 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    14941 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15001 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15061 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15121 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15181 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    15241 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    15301 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    15361 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    15421 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    15481 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    15541 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    15601 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    15661 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    15721 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    15781 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    15841 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    15901 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    15961 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16021 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16081 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16141 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16201 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    16261 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    16321 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    16381 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    16441 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    16501 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    16561 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    16621 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    16681 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    16741 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    16801 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    16861 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    16921 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    16981 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17041 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17101 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17161 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    17221 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    17281 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    17341 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    17401 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    17461 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    17521 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    17581 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    17641 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    17701 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    17761 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    17821 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    17881 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    17941 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18001 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18061 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18121 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18181 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    18241 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    18301 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    18361 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    18421 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    18481 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    18541 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    18601 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    18661 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    18721 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    18781 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    18841 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    18901 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    18961 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19021 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19081 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19141 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19201 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    19261 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    19321 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    19381 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    19441 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    19501 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    19561 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    19621 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    19681 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    19741 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    19801 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    19861 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    19921 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    19981 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20041 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20101 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20161 cattgttcac ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta
    20221 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    20281 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    20341 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    20401 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    20461 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    20521 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    20581 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    20641 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    20701 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    20761 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    20821 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    20881 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    20941 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21001 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21061 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21121 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21181 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    21241 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    21301 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    21361 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    21421 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    21481 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    21541 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    21601 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    21661 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    21721 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    21781 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    21841 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    21901 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    21961 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22021 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22081 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22141 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22201 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    22261 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    22321 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    22381 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    22441 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    22501 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    22561 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    22621 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    22681 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    22741 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    22801 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    22861 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    22921 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    22981 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23041 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23101 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23161 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    23221 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    23281 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    23341 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    23401 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    23461 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    23521 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    23581 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    23641 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    23701 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    23761 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    23821 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    23881 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    23941 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24001 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24061 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24121 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24181 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    24241 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    24301 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    24361 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    24421 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    24481 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    24541 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    24601 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    24661 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    24721 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    24781 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    24841 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    24901 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    24961 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25021 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25081 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25141 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25201 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    25261 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    25321 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    25381 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    25441 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    25501 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    25561 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    25621 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    25681 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    25741 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    25801 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    25861 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    25921 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    25981 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26041 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26101 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26161 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    26221 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    26281 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    26341 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    26401 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    26461 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    26521 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    26581 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    26641 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    26701 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    26761 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    26821 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    26881 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    26941 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27001 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27061 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27121 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27181 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    27241 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    27301 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    27361 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    27421 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    27481 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    27541 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    27601 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    27661 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    27721 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    27781 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    27841 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    27901 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    27961 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28021 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28081 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28141 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28201 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    28261 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    28321 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    28381 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    28441 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    28501 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    28561 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    28621 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    28681 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    28741 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    28801 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    28861 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    28921 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    28981 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29041 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29101 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29161 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    29221 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    29281 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    29341 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    29401 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    29461 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    29521 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    29581 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    29641 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    29701 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    29761 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    29821 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    29881 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    29941 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30001 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30061 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30121 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30181 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    30241 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    30301 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    30361 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    30421 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    30481 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    30541 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    30601 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    30661 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    30721 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    30781 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    30841 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    30901 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    30961 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31021 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31081 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31141 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31201 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    31261 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    31321 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    31381 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    31441 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    31501 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    31561 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    31621 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    31681 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    31741 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    31801 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    31861 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    31921 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    31981 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32041 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32101 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32161 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    32221 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    32281 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    32341 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    32401 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    32461 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    32521 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    32581 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    32641 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    32701 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    32761 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    32821 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    32881 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    32941 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33001 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33061 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33121 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33181 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    33241 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    33301 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    33361 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    33421 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    33481 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    33541 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    33601 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    33661 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    33721 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    33781 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    33841 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    33901 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    33961 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34021 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34081 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34141 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34201 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    34261 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    34321 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    34381 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    34441 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    34501 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    34561 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    34621 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    34681 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    34741 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    34801 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    34861 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    34921 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    34981 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35041 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35101 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35161 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    35221 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    35281 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    35341 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    35401 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    35461 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    35521 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    35581 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    35641 acagcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    35701 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    35761 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    35821 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    35881 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    35941 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36001 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36061 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36121 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36181 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    36241 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    36301 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    36361 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    36421 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    36481 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    36541 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    36601 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    36661 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    36721 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    36781 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    36841 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    36901 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    36961 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37021 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37081 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37141 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37201 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    37261 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    37321 tcagaagctg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    37381 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    37441 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    37501 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    37561 cttattagat tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    37621 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    37681 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    37741 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    37801 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    37861 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    37921 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    37981 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38041 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38101 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38161 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    38221 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    38281 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    38341 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    38401 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    38461 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    38521 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    38581 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    38641 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    38701 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    38761 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    38821 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    38881 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    38941 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39001 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39061 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39121 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39181 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    39241 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    39301 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    39361 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    39421 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    39481 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    39541 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    39601 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    39661 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    39721 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    39781 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    39841 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    39901 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    39961 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40021 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40081 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40141 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40201 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    40261 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    40321 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    40381 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    40441 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    40501 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    40561 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    40621 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    40681 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    40741 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    40801 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    40861 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    40921 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    40981 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41041 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41101 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41161 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    41221 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    41281 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    41341 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    41401 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    41461 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    41521 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    41581 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    41641 cggtgtaccc aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    41701 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    41761 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    41821 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    41881 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    41941 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42001 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42061 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42121 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42181 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    42241 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    42301 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    42361 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    42421 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    42481 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    42541 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    42601 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    42661 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    42721 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    42781 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    42841 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    42901 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    42961 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43021 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43081 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43141 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43201 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    43261 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    43321 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    43381 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    43441 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    43501 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    43561 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    43621 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    43681 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    43741 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    43801 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    43861 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    43921 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    43981 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44041 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44101 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44161 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    44221 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    44281 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    44341 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    44401 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    44461 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    44521 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    44581 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    44641 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    44701 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    44761 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    44821 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    44881 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    44941 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45001 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45061 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45121 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45181 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    45241 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    45301 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    45361 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    45421 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    45481 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    45541 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    45601 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    45661 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    45721 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    45781 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    45841 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    45901 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    45961 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46021 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46081 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46141 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46201 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    46261 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    46321 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    46381 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    46441 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    46501 tgcacagttc aacgcattac gttg
//
LOCUS       DAZPFP010000016        42558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPFP010000016 DAZPFP010000000
VERSION     DAZPFP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.016"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..803
                     /locus_tag="ACHUR4_000819"
     CDS             <1..803
                     /locus_tag="ACHUR4_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454516.1"
                     /translation="TRSELSLINLLNSLNEVELMRKRLAFLMTAPEYACYNSQYCYED
                     KVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDIIISNTGEKFSEVGLTKYAQH
                     LASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMI
                     QYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAI
                     TKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(863..1354)
                     /gene="yajQ"
                     /locus_tag="ACHUR4_000820"
     CDS             complement(863..1354)
                     /gene="yajQ"
                     /locus_tag="ACHUR4_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1454517.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1522..2433
                     /gene="panE"
                     /locus_tag="ACHUR4_000821"
     CDS             1522..2433
                     /gene="panE"
                     /locus_tag="ACHUR4_000821"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1454518.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2396..2986
                     /gene="yajL"
                     /locus_tag="ACHUR4_000822"
     CDS             2396..2986
                     /gene="yajL"
                     /locus_tag="ACHUR4_000822"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1454519.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3040..4488)
                     /gene="thiI"
                     /locus_tag="ACHUR4_000823"
     CDS             complement(3040..4488)
                     /gene="thiI"
                     /locus_tag="ACHUR4_000823"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1454520.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4694..4936
                     /gene="xseB"
                     /locus_tag="ACHUR4_000824"
     CDS             4694..4936
                     /gene="xseB"
                     /locus_tag="ACHUR4_000824"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1454521.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4936..5835
                     /gene="ispA"
                     /locus_tag="ACHUR4_000825"
     CDS             4936..5835
                     /gene="ispA"
                     /locus_tag="ACHUR4_000825"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1454522.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5860..7722
                     /gene="dxs"
                     /locus_tag="ACHUR4_000826"
     CDS             5860..7722
                     /gene="dxs"
                     /locus_tag="ACHUR4_000826"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1454523.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7777..8694
                     /gene="yajO"
                     /locus_tag="ACHUR4_000827"
     CDS             7777..8694
                     /gene="yajO"
                     /locus_tag="ACHUR4_000827"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1454524.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8805..9323)
                     /gene="pgpA"
                     /locus_tag="ACHUR4_000828"
     CDS             complement(8805..9323)
                     /gene="pgpA"
                     /locus_tag="ACHUR4_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1454525.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9301..10278)
                     /gene="thiL"
                     /locus_tag="ACHUR4_000829"
     CDS             complement(9301..10278)
                     /gene="thiL"
                     /locus_tag="ACHUR4_000829"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1454526.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10356..10775)
                     /gene="nusB"
                     /locus_tag="ACHUR4_000830"
     CDS             complement(10356..10775)
                     /gene="nusB"
                     /locus_tag="ACHUR4_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1454527.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10795..11265)
                     /gene="ribE"
                     /locus_tag="ACHUR4_000831"
     CDS             complement(10795..11265)
                     /gene="ribE"
                     /locus_tag="ACHUR4_000831"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1454528.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11354..12457)
                     /gene="ribD"
                     /locus_tag="ACHUR4_000832"
     CDS             complement(11354..12457)
                     /gene="ribD"
                     /locus_tag="ACHUR4_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1454529.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12461..12910)
                     /gene="nrdR"
                     /locus_tag="ACHUR4_000833"
     CDS             complement(12461..12910)
                     /gene="nrdR"
                     /locus_tag="ACHUR4_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1454530.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13061..13600
                     /locus_tag="ACHUR4_000834"
     CDS             13061..13600
                     /locus_tag="ACHUR4_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1454531.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13898..14782
                     /gene="tsx"
                     /locus_tag="ACHUR4_000835"
     CDS             13898..14782
                     /gene="tsx"
                     /locus_tag="ACHUR4_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1454532.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14959..15306)
                     /gene="yajD"
                     /locus_tag="ACHUR4_000836"
     CDS             complement(14959..15306)
                     /gene="yajD"
                     /locus_tag="ACHUR4_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1454533.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15435..16406)
                     /gene="secF"
                     /locus_tag="ACHUR4_000837"
     CDS             complement(15435..16406)
                     /gene="secF"
                     /locus_tag="ACHUR4_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1454534.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16417..18264)
                     /gene="secD"
                     /locus_tag="ACHUR4_000838"
     CDS             complement(16417..18264)
                     /gene="secD"
                     /locus_tag="ACHUR4_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1454535.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18292..18624)
                     /gene="yajC"
                     /locus_tag="ACHUR4_000839"
     CDS             complement(18292..18624)
                     /gene="yajC"
                     /locus_tag="ACHUR4_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1454536.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18647..19774)
                     /gene="tgt"
                     /locus_tag="ACHUR4_000840"
     CDS             complement(18647..19774)
                     /gene="tgt"
                     /locus_tag="ACHUR4_000840"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1454537.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19829..20899)
                     /gene="queA"
                     /locus_tag="ACHUR4_000841"
     CDS             complement(19829..20899)
                     /gene="queA"
                     /locus_tag="ACHUR4_000841"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1454538.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20992..21565
                     /gene="acpH"
                     /locus_tag="ACHUR4_000842"
                     /pseudo
     CDS             20992..21565
                     /gene="acpH"
                     /locus_tag="ACHUR4_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21570..23387)
                     /gene="malZ"
                     /locus_tag="ACHUR4_000843"
     CDS             complement(21570..23387)
                     /gene="malZ"
                     /locus_tag="ACHUR4_000843"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1454539.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23543..24916)
                     /gene="proY"
                     /locus_tag="ACHUR4_000844"
     CDS             complement(23543..24916)
                     /gene="proY"
                     /locus_tag="ACHUR4_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1454540.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24992..26311)
                     /gene="brnQ"
                     /locus_tag="ACHUR4_000845"
     CDS             complement(24992..26311)
                     /gene="brnQ"
                     /locus_tag="ACHUR4_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1454541.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26718..28013)
                     /gene="phoR"
                     /locus_tag="ACHUR4_000846"
     CDS             complement(26718..28013)
                     /gene="phoR"
                     /locus_tag="ACHUR4_000846"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1454542.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28071..28760)
                     /gene="phoB"
                     /locus_tag="ACHUR4_000847"
     CDS             complement(28071..28760)
                     /gene="phoB"
                     /locus_tag="ACHUR4_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1454543.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28950..30152
                     /gene="sbcD"
                     /locus_tag="ACHUR4_000848"
     CDS             28950..30152
                     /gene="sbcD"
                     /locus_tag="ACHUR4_000848"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1454544.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30149..33292
                     /gene="sbcC"
                     /locus_tag="ACHUR4_000849"
     CDS             30149..33292
                     /gene="sbcC"
                     /locus_tag="ACHUR4_000849"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1454545.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33418..34599
                     /gene="araJ"
                     /locus_tag="ACHUR4_000850"
     CDS             33418..34599
                     /gene="araJ"
                     /locus_tag="ACHUR4_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1454546.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34742..35650)
                     /gene="mak"
                     /locus_tag="ACHUR4_000851"
     CDS             complement(34742..35650)
                     /gene="mak"
                     /locus_tag="ACHUR4_000851"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1454547.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35775..36686
                     /gene="rdgC"
                     /locus_tag="ACHUR4_000852"
     CDS             35775..36686
                     /gene="rdgC"
                     /locus_tag="ACHUR4_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1454548.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36764..37219)
                     /locus_tag="ACHUR4_000853"
     CDS             complement(36764..37219)
                     /locus_tag="ACHUR4_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454549.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37216..37551)
                     /locus_tag="ACHUR4_000854"
                     /pseudo
     CDS             complement(37216..37551)
                     /locus_tag="ACHUR4_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37706..37990)
                     /gene="ppnP"
                     /locus_tag="ACHUR4_000855"
     CDS             complement(37706..37990)
                     /gene="ppnP"
                     /locus_tag="ACHUR4_000855"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1454550.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38062..38739)
                     /locus_tag="ACHUR4_000856"
     CDS             complement(38062..38739)
                     /locus_tag="ACHUR4_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1454551.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38997..39188)
                     /gene="yaiA"
                     /locus_tag="ACHUR4_000857"
     CDS             complement(38997..39188)
                     /gene="yaiA"
                     /locus_tag="ACHUR4_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1454552.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39238..39762)
                     /gene="aroL"
                     /locus_tag="ACHUR4_000858"
     CDS             complement(39238..39762)
                     /gene="aroL"
                     /locus_tag="ACHUR4_000858"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1454553.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39945..40403)
                     /locus_tag="ACHUR4_000859"
     CDS             complement(39945..40403)
                     /locus_tag="ACHUR4_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1454554.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40523..41332
                     /gene="proC"
                     /locus_tag="ACHUR4_000860"
     CDS             40523..41332
                     /gene="proC"
                     /locus_tag="ACHUR4_000860"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1454555.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41349..42464)
                     /gene="adrA"
                     /locus_tag="ACHUR4_000861"
                     /gene_synonym="dgcC"
     CDS             complement(41349..42464)
                     /gene="adrA"
                     /locus_tag="ACHUR4_000861"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1454556.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10730 a        11362 c        10614 g         9852 t
ORIGIN      
        1 caacaagaag tgaattaagc ctcattaatt tattaaattc attaaatgag gtggaattga
       61 tgagaaaacg tctggcgttt ctgatgacag caccagaata cgcttgttat aatagtcagt
      121 attgttatga agacaaagtc agcgcattaa aaatcctgta tcgtcatcat gacaatgagc
      181 taatggacaa atatttcacg actcttctta aagagatcag taatcaacca ttagaagact
      241 gggatataat aataagtaat actggtgaaa aatttagtga agtgggtcta accaaatatg
      301 ctcaacatct cgcatctttc gctgaagaat tttatataaa agagcaaaaa tcacattctg
      361 aaagtgaaat gagaggacta ttttcatcac ttgccgaatt atatagtttt ggtaatgata
      421 ctgccagcgc aaaccgagtg ttccatcagc atgtccctgc cttcaataca gatcatatga
      481 ttcaatattt tataaatgcc aaagaatgga acaatgccag ggaattattg ataaaagagg
      541 aaatgttggg tatgcataat ctgatattgt tggagaatat ttgtatgcaa aaaaatgcag
      601 aatgcatggc acacattacc tttacgctta ataaattaac aacacaacca gcaataacaa
      661 aaatagatag cgtgggtaat gagcaacttt atcagattgg caaaatctac cataagttgg
      721 aaattaaacc tgagccagag caacaggttt taattcagtc gctttatgac cgggcatcag
      781 gaagcgcctc tgcaactcaa taaatgcatg gtcaaaaggg ccaccacggt ggccctgaca
      841 atcagtagct atatccagaa agttaatcgc ggaagttttt gaactggaac ggctgaccga
      901 gatcgccacc gcgcaccatc gccatgacag cctgcaaatc atcacgagat ttgccagtca
      961 cgcggatctc atcgccctga atttgcgcct gcactttcag tttgctgtct ttgatcatct
     1021 tgacgatttt tttctgagtg gcgctctcaa tgccctgttt cagtttcgct tccacaaacc
     1081 aggttttacc gctatgaacg atattttccg gtacatccag tgaactgcct tcaatgccgc
     1141 gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaag tcggactcgc
     1201 tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca acgttacgga
     1261 agtcaaaacg ggactccact tcgcgactcg cgttatcgac cgcgttacgt gcttcctgaa
     1321 gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc atttttgatg
     1381 cgaagcataa tacctgcaaa gttaaataac caacagtcct gcaccaggac gtatccctta
     1441 attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa cacccggagt
     1501 ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc cttagggcaa
     1561 ttatggctta cagcactttg taaacagggt catgaagttc agggctggct gcgtgtaccg
     1621 caaccttatt gtagtgtgaa tctggttgag acagatggtt cgatatttaa cgaatcgctg
     1681 accgccaacg atcccgattt tctcgccacc agcgatctgc tcctggtgac gctgaaagca
     1741 tggcaggttt ccgatgccgt caaaagcctc gcatccacac tgcctgtaac tacgccaata
     1801 ctgttaattc acaacggcat gggcaccatc gaagaattgc aaaacattca gcagccatta
     1861 ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat tcatgtggca
     1921 aacggaatca cgcatatcgg tccggcacgg caacaggatg gcgattacag ttatctggcg
     1981 gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg cgccgagctg
     2041 tggcgcaagc tggccgtcaa ctgcgtgatt aatccactga ctgccatctg gaattgcccg
     2101 aacggtgaat tacgtcatca tccgcaagaa attatgcaaa tatgcgaaga agtcgcggca
     2161 gtgatcgaac gcgaagggca tcatacttca gcggaagatt tgcgtgatta cgtgatgcag
     2221 gtgattgatg ccacagcaga aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc
     2281 cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc gcatgggatt
     2341 gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag
     2401 cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg tcaccacgat
     2461 caacctcttg gttcgcggcg gtatcaaagt caccactgcc agcgtcgaca gcgatggcaa
     2521 cctggcaatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaagt
     2581 ggctgatggc gaatatgacg tgatcgtgct gcctggtggc attaaaggca cggagtgttt
     2641 tcgcgatagc actctgctgg ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt
     2701 cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct ttccgattgg
     2761 caatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat ggcaggacaa
     2821 gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg gtacagctat
     2881 cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc atgaagtggc
     2941 atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc ggatgcggca
     3001 aacgtcgcat ctgaccagat gcgacgtaaa aactacaaat tacgggcgat ataccttcac
     3061 attgttaaag ccttgctcgc gcagatagag cgcctgcaga cggctcatca ccccgcgctc
     3121 acaccacagc agccaggttt tgttctggtc gagatcgcca aatttggtgc tcagtttata
     3181 gaacggcaga gaaaccacat caatcccttc gactttcagt ggcttatctt cctgttcatc
     3241 gacagaacgg atatcgagaa tcacgtcgtt cgggccgaag ccgttgacgg tttccacttc
     3301 caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat tcgcttcctc
     3361 aaccacttta tcgagaatac tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt
     3421 aaccgctttc accgtcgggc ttttggagat cacgccacaa tattccggca tcgtgcgagc
     3481 aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt cgtaagaaat
     3541 cagcggacgc aggatcagcg tgtcagagac gttatcaatc aggcgcaggt tggtcagcgt
     3601 ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc
     3661 agccacttta gatgcggcac gcaccatcat acgtttgagg ataacgccca tctgaccgtc
     3721 gtcgattttc tcgagaattt ccccgacaac cggttcgaaa ttaatggcga caaaacgcac
     3781 gcggtgggag ctgccaaagc ggttccacag ataatgcgcc acctgacgca cgccaatttc
     3841 atgcgctgcg ccgccgaggt taaagaagca gtaatgcacg cggcagccgc gacgcatcaa
     3901 catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca catcttcctg
     3961 ggtgccaatc gggaaaccac caataccttc gtagcgacct ttaatcagca ggaggcgatc
     4021 gtcttccact tccagatgaa cagttacatc cgggttagtc agcttcacgc gcgcggattc
     4081 aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc taaaatcatg
     4141 tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat cgcgatactg
     4201 aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag
     4261 aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagtcagac gctggttttc
     4321 atctttcgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg tctcatcata
     4381 gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca agcgcacaga
     4441 ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg cggcaatggt
     4501 tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac acctgcggct
     4561 gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca ccttaccatt
     4621 gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa atattgagag
     4681 tcagacattc attatgccga agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag
     4741 cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg aagaggcgct
     4801 gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat tacaacaagc
     4861 cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa ccccttttac
     4921 accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa gcaggccaac
     4981 caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt ggtcgaaacc
     5041 atgcagtatg gcgcattatt aggtggtaag cgcctgcgac ctttcctggt ttatgccacc
     5101 ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgctgc cgtagagtgt
     5161 atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga cgatctgcgt
     5221 cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac
     5281 gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga agtgtcggat
     5341 cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc cggaatgtgc
     5401 ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga cgcgcttgag
     5461 cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct tggtgcatta
     5521 agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtacgc agagagcatc
     5581 ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac tgcaacgttg
     5641 ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc acttctgggt
     5701 cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca ggcgctgaaa
     5761 caactggctg aacagtcact cgatacctcg gcactggaag cgctagcgga ctacatcatc
     5821 cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga tattgccaaa
     5881 tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc gaaagagagt
     5941 ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag ccgttccagc
     6001 gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca ctatgtctac
     6061 aacaccccat ttgaccaatt gatttgggat gtgggacatc aggcttatcc gcataaaatt
     6121 ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggtct gcacccgttc
     6181 ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc aacctccatc
     6241 agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg ccgcaccgtc
     6301 tgtgtgattg gcgacggcgc gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg
     6361 ggcgatattc gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa
     6421 aatgttggcg cgcttaacaa ccatctggct cagctgcttt ccggtaagct ttactcttca
     6481 ctgcgcgaag gcgggaaaaa agttttctct ggcgtgccgc ccattaaaga gctgctcaaa
     6541 cgtaccgaag aacatattaa aggcatggtg gtgcctggca cgttgtttga agagctggga
     6601 tttaactaca tcggcccggt ggacggtcac gatgtgctgg ggcttatcac cacgctaaag
     6661 aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt
     6721 tatgaaccgg cagaaaaaga cccgatcact ttccacgccg tgcctaaatt tgatccctcc
     6781 agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat ctttggcgac
     6841 tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc ggcgatgcgt
     6901 gaaggttccg gcatggtcga gttttcacgt aaattcccgg atcgttactt cgacgtggct
     6961 attgccgagc aacacgcagt gacctttgcc gccggtctgg cgattggtgg atacaaaccc
     7021 attgtcgcga tctactccac cttcctgcaa cgcgcctatg atcaggtgct gcatgacgtg
     7081 gcaattcaaa agctaccggt cctgttcgcc attgaccgcg cgggcattgt tggtgctgac
     7141 ggtcaaactc accagggcgc ttttgatctc tcttacctgc gctgcatacc agaaatggtc
     7201 attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg ctatcactat
     7261 aacgatggcc cgtccgcggt gcgctacccg cgcggcaacg cggttggcgt ggaactgacg
     7321 ccgctggaaa aactaccaat tggcaaaggc attgtgaagc gtcgtggcga gaaactggtg
     7381 atccttaact ttggtacgct gatgccagaa acggccaaag tcgccgaatc gctgaacgct
     7441 acgctggtcg atatgcgttt tgtgaaacca cttgatgaag cgttaattct ggaaatggcc
     7501 gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg cgcaggtagc
     7561 ggagtgaacg aagtgctgat ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg
     7621 ccggacttct ttattccgca aggaactcag gaagaaatgc gcgccgaact cggcctcgat
     7681 gccgccggta tggaagccaa aatcaaggcc tggttggcat aatccctact ccactcctgc
     7741 tatgcttaag aaattattca tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa
     7801 accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc
     7861 ggtaatcacg catggacact gccggaagag agcagccgtc ccataattaa acgcgcgctg
     7921 gaaggtggca taaatttctt tgataccgcc aatagctatt ccgatggtag cagcgaagag
     7981 atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtagtcgt tgccaccaaa
     8041 gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct
     8101 atcaacgaca gcctgcgacg tctcggcatg gattatgtcg atatcctgca aattcatcgc
     8161 tgggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc
     8221 gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggcgctg
     8281 gaactacaaa aacagcactg ctgggcgcag tttgtcagta tgcaggatca ctataatctg
     8341 atttatcgcg aagaagagcg cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg
     8401 attccgtgga gcccgctggc gcgggggcga ctgacgcgtc catggggaga aactaccgca
     8461 cgactggtgt ctgatgaggt ggggaaaaat ctctataaag aaagcgatga aaatgacgcg
     8521 cagatcgcag agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt
     8581 gcgctggcct ggttgttaag taaaccgggc attgccgcac cgattatcgg aacttcgcgg
     8641 gaagaacagc ttgatgagct gctgaacgcg gtggatatca ctttgaagcc ataacagatt
     8701 gccgagctgg aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg
     8761 cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc acaactacga cagaataccc
     8821 agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaatcacccc ggcgacaata
     8881 tcgtcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac
     8941 ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca
     9001 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
     9061 ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag
     9121 atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag
     9181 aaagggatcg ccgccagcga tcccatcgag ccaggaacga ttgggcttaa tccacttccg
     9241 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
     9301 ttatggcgtg acaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg
     9361 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
     9421 aaggtgtccg agagcaacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa
     9481 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
     9541 agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc
     9601 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
     9661 cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat ggacggagat gacgtttgat
     9721 caagtagtca gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
     9781 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccaga
     9841 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg
     9901 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
     9961 actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt
    10021 cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt
    10081 ataagccaga tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca ccagcgtatc
    10141 agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat cgtcgccgat
    10201 gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga
    10261 gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca
    10321 tggaacagtc ttccgtgaat ctaccggcct ggatattact ttttgttagg gcgaatcaca
    10381 ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat
    10441 ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcact acgtttagac
    10501 agttcgtaca gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg
    10561 cgggacaggt agggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc
    10621 agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc caggaactgg
    10681 tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca
    10741 cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc
    10801 cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt
    10861 gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac
    10921 cccaaaagca accggaattt cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc
    10981 accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca gcgcaatcac
    11041 cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc
    11101 aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc
    11161 aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc
    11221 gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt
    11281 tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt
    11341 tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct acatgacgta
    11401 tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc agcgagcata
    11461 atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc tcatcgacta
    11521 aacccgcctg tagcaatacg ccagcgagcg ttggccccgc ttccacccag atgctgttaa
    11581 tctgctgttt acccagttgc atcatcagta caaccagatc cagatgacct ttatgctctg
    11641 gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga
    11701 accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat
    11761 caatcacaat acgtactggc tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt
    11821 catccagttc agaccaacgc actgttaagg ctggatcatc cgccagcacc gtggcgctgc
    11881 tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg
    11941 aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg gccatcaagc gatgcgccaa
    12001 gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt
    12061 gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt tgcagacggt
    12121 aaagtccacg cccagcgacc tgcgggttag gatcttgcat cgctgcgacc acgcgcgcta
    12181 cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg gccatgatgg ctacagggtt
    12241 cgagtgtcac atacgccgtt gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta
    12301 cttcggcatg tggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt
    12361 tgacaatgac gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca
    12421 gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga
    12481 tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat
    12541 aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg
    12601 gtacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt gccatttcaa
    12661 cgtaatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct
    12721 cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg
    12781 ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg cggcgtacgg
    12841 atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg aaacagaatg
    12901 ggcaatgcat acggcgtcct gaccaggtgg ttaacggaaa tctattttac cctgaactga
    12961 cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc tgcttttacc
    13021 ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta acgtttttcg
    13081 actgctcctg ctgggaagcc tgttcagcct tagcgcctgt gtgcagcaaa gtgaagtgcg
    13141 acagatgaaa cacagcgtca gcacgctgaa ccaggagatg acgcaactca atcaagaaac
    13201 agtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc agcagtgggg tttaccttct
    13261 gcctggagcg aaaacaccgg caagactgga aagccagatc ggtactttac gtatgtcgct
    13321 ggtgaatatt acgcctgata cagatggcac cacccttaca ctacgtattc agggtgagtc
    13381 gaatgatccc ttgcccgcat tcagcggaac tgttgaatat gggcaaattc agggaacaat
    13441 agacaacttt caggaaatca atgtgcagaa tcaattgatt aatgcccctg ccagcgtcct
    13501 cgcccccagc gatgttgata ttccgttaca attaaagggg atttctgtgg atcagttagg
    13561 ctttgtacgt atccacgaca ttcaacctgt tatgcagtaa acgtatttcg ggacgatttt
    13621 gtgcgtcccg caacatcttc cccgtcattt tgttactctg cttacatcac ctggattgat
    13681 agtaaaagtt tgcaacaagg gcgaaagtca gtacaatccc cgcccgaatg tgtgtaaacg
    13741 tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa
    13801 tgatttttat aataggctcc tctgtatacg aaatatttag aaacgcaatt tgcgcctttt
    13861 tcactcccgc aagggatttt caaacagtgg catacatatg aaaaaaacat tactggcagc
    13921 cggtgcggta ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc
    13981 gcagtatctt tccgactggt ggcaccagag cgttaacgtt gtcggaagct atcacacccg
    14041 tttcggaccg cagatccgca acgataccta ccttgagtac gaagcattcg ctaaaaaaga
    14101 ctggttcgac ttctatggtt atgcggatgc gccggtattc ttcggcggta actccgatgc
    14161 taaaggtatc tggaaccacg gttctccgct gtttatggaa atcgaaccac gtttctccat
    14221 cgacaagctg accaatactg accttagctt cggtccgttc aaagagtggt acttcgcgaa
    14281 caactacatt tacgacatgg gtcgtaataa agatggtcgc cagagcacct ggtacatggg
    14341 tctgggtacc gatatcgaca ctggcctgcc gatgagcctg tccatgaacg tctatgcgaa
    14401 ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa
    14461 aattaaatac tttgtgccga ttaccgatct gtggggcggt cagctgagct acatcggctt
    14521 caccaacttc gactggggtt ccgatttagg ggatgacagc ggtaacgcaa tcaacggtat
    14581 taagacccgt actaataact ctatcgcttc cagccatatt ctggctctga actacgatca
    14641 ctggcactac tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga acgacgatgc
    14701 agaactgaac ttcggcaacg gcaacttcaa cgttcgctct accggctggg gtggttacct
    14761 ggtagtaggt tacaacttct gattatgaaa atgccgggat ttattcccgg catttctgat
    14821 tgttaacaac gtgtgctttg ttcatgccgg atgcggcgta aacgctttgt ccggcccaca
    14881 aaagcacgta aattcaatat attgctgaaa taacgtaggc ctgataagcg tagcgcatca
    14941 ggccatttta cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg
    15001 gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc
    15061 atactgatcc gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacacaacaa
    15121 ttcccagtta ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacggt
    15181 aagttcacgc aggttagaat aaacaaactc gcgggaacag cgaccgcaga cccacggata
    15241 gatttttaat gctttttcgc gatagccgct ttctaaccgt gcatagtttt ttgggatgat
    15301 agccatgaaa agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg
    15361 attttcgcaa tctccataca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat
    15421 caacgggaac ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc
    15481 tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccaacgcaga tgctacgtag
    15541 atggaagatg cagtgccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt
    15601 accggaccac cgaagagata cagcatcagg ataaccatca aggtagtacc ggatgtgatc
    15661 aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga
    15721 cggatcttgc ggaagttttc acgaattcgg tccgatacca cgatactgtc gttaagcgag
    15781 taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac
    15841 gacaaaatac ccagcgtaat gataacgtcg tgcgtcagcg caataaccac ccctgccgcc
    15901 agtcgccact caaagcggaa acctacgtac acgaggatag acagcagcgc tgccatcaac
    15961 gccatcgcac cggtttgcgc aaggtcagcc cccacgctcg gaccgacgaa ctcaatacgc
    16021 ttcactgctg cattctgatt ggtggattcg ttaatcacct tcagaacttg gctgcccagc
    16081 acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta
    16141 ccaaagtttt gcagcattgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg
    16201 tcaatttcag ccggtttttc gagcgtaatt tcgataaccg taccaccggt gaaatccagc
    16261 ccccagttaa agccgcgcac gcccataata acgatagcag cgattaacag cagaccagag
    16321 atgccgaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt
    16381 tgttcaacag tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg
    16441 cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga
    16501 cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc
    16561 gtacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc
    16621 gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt
    16681 aatacgttcg ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc
    16741 cgcgatacct ggcatactca gtgttgcgcc tggcaacagc gacataatac cgacgatcaa
    16801 gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat
    16861 catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt
    16921 ctgcataccc agggtcgggc caattgtacg ttcttcaaca atctgaatcg gcgcgatcaa
    16981 cgcaccggca cgcagcagca gtgacagctg acgggcttcg ttcgggttgt tgatgccggt
    17041 gatacggaag ctgttaccca gacgagactg gatgttggcg atgttaatca cctcttcctg
    17101 tttcaccaga accgcacgac catttgcatc tttcttaccg ctgtctttgt actccacaaa
    17161 cagggttgcc atcggtttgc cgatgttgtc cttggtgaag ttagacataa tgttgccacc
    17221 agcgctatcg agcgagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga
    17281 gtcggtgata tggtcaccag tcagaattac gcgtttgtac agcacaaccg gctgaccttc
    17341 gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc
    17401 aacattggtg tttaccagac ggaattccag cgttgccgtc gcgcccagaa tctctttcgc
    17461 acgcgcagtg tcctgaatac cgggcagttc aacaacgata cggtcagcac cctgacgctg
    17521 aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat taatgttctg
    17581 ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg
    17641 gttgctgccc tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc
    17701 ttcatcacga gctttagcat cgcggaaagt gatgctcagg ccgtagttgt tttctttacg
    17761 aacagtggta tacgggatgc ccttttcgcg caggtcactg cgtaggctat cgatattttg
    17821 ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc
    17881 acgcaggtca aggccgagct tcatcggctc agcatgaata gctgccagcc agcgcggcgt
    17941 tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc
    18001 acgagcgcgc aactgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag
    18061 tgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt
    18121 ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa
    18181 caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt
    18241 ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc
    18301 cttcatggtg cctttcggca ggacggcagc tacgaagtca cgtttaataa ctacttcagt
    18361 ggtgtcattc agcgcgatag caatgtagcc gttttccgct actttggtta cgcgaccaac
    18421 caggccaccg ttcgtcagaa cttcatcacc tttggcaatg gagtccatca gctttttgtg
    18481 ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac
    18541 caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac
    18601 cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca
    18661 aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac
    18721 cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa
    18781 tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc gagatgatgc aagtaagcgc
    18841 gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct
    18901 tatatttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg
    18961 cgttgcgggt tggcattacg cagtcaaaca tatcgatacc gcgacgtacg ccttcaagca
    19021 ggtcttccgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcggac
    19081 acacatgctc cagaatacgg tgcatatctg ctttcggctc acccacagcc agaccgccga
    19141 cagcgtagcc atcaaagccg atatctacca gacctttaac agatatatca cgtaaatctt
    19201 cgtaaatact gccctgaatt ataccaaata acgcattttt gttaccaaga ctgtcaaaac
    19261 gctcacggct acgcttcgcc caacgcagag acatctccat agagcgtttt gcgtaatccc
    19321 agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt
    19381 actgaatctc catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac
    19441 ggaagtgaac gccctgttcg gtgattttac gaatatcacc aaggctgaag acctggaagc
    19501 cgccggagtc ggtaagaatc ggtcctttcc actgcataaa atcgtgcaga tcgccgtgca
    19561 gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt
    19621 gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg taggtgccaa
    19681 caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg
    19741 cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca
    19801 gtccaacgtt taaaccagcg ccgcggaatt actccccgac gcgctcatta attgcctgcg
    19861 gattgtacgt gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg
    19921 ccgctttata ggcattcatg gtgtgttgat aaccggcaaa ggccgaaacc agcataatca
    19981 gcgtcgactc tggcaagtgg aagttcgtca ccagcgcatc gaccactttg tactggaagc
    20041 ccggatagat aaagatttgg gtatcgtcaa agaacggttc gataagatcg ttttttgccg
    20101 cctgtgccgc gctttccagc gaacgtactg aagtggtgcc aaccgcaatc acccggttac
    20161 cgcgcgcttt cgccgccagt accgcgtcta ccacatcctg cggtacttca gcgtattccg
    20221 agtgcatgat gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa
    20281 cgtgcaacgt cacaaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggct
    20341 cgtcaaaatg cagaccagcg gtcggggctg caaccgcgcc cggtttttcg ctataaacgg
    20401 tttgataaag ttcgcggtca gcgtcttcgt ccggacgatc gatatacggc ggcagcggca
    20461 tatggccgat gctgttgagg atatccagta ccgagcgttc atcattaaat tcgacttcaa
    20521 acagtgcgcc gtggcgcgcg gtcattgttg cgttgatact ttcgtcatcg cccagcaaca
    20581 gttctgcgcc aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt
    20641 cgagcatccg ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacagac
    20701 gcgccgggat cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga
    20761 gtaaatcggt gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac
    20821 gacagctact gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaagg
    20881 agaaatcggt aacgcgcatg acactgactc agactaaaat aagagtcggg tagtctagtg
    20941 ccggggcgct ctccctgcaa cctttacccc cctttggata gaatataatc gatgaatttt
    21001 ttagctcacc tgcatttagc ccatctcgcg gaaagctcgc tttctggcaa tttactggct
    21061 gatttcgtac gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat
    21121 atgcatcgac gtattgactg acaatctgcc ggaagtccgc gaagcacagg agtggtttcg
    21181 tagtgaaacg cgccgcgttg cgcctattac gctggatgtc atgtgggatc actttctttc
    21241 ccgccactgg tcgcagctgt cgccggactt tccgctacag gaatttgtct gttatgcccg
    21301 cgagcaagtg atgacgattt tgccggactc accgccacgt tttatcaatc tgaacaatta
    21361 cttgtggtca gagcagtggc tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt
    21421 aaacggcatg gcaagccgcc gcccacgtct ggatgccctg cgtgactcct ggtacgattt
    21481 agacgctcat tatgacgccc tcgaaacccg cttctggcag ttttatccgc ggatgatggc
    21541 gcaggcgtca cacaaggcgt tataaaacgt tagttcatcc ataccgtagc cgaaatggca
    21601 ggcaaagcca gaatcccgtc agtcagttgc ccatgccctt ctttgcattg ccattgcacg
    21661 gcattgagaa acggtgacgc gggtagcacc acttcactgg cctcgccacg gttgattgcc
    21721 accagtacac gttgctgatt cagcacgcgg acaaatacca ctacgttatc ttccgcatac
    21781 agcacctgac agccgccacg acgcagcgcc tgacttttct tacgcagcac aatcattcgc
    21841 cggtacagcg cgaataacgc cgtatcctgc ttttccacct gccaggggaa cggtttacgg
    21901 caaaacggat cgtttttacc atccagtccc acttcatcac cgtaataaat gcacggtaca
    21961 ccaggccagg tgaacagcca gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc
    22021 agcgttttaa atcgcgcagt atcgtggcta tcgagctgat taaacatacg taattgttgt
    22081 tgatgagaaa gccctgcgcg gtaattatcc atccaggcca tacaggtttg ggcgtcaatt
    22141 tgctgcggat cataagagat gtcggtattg gcaagaaatc cccacaacgg gaatgtgaag
    22201 ccacgatagt tcatggcagc atcttccaca tcggcctgta accattgccg tgcatcgcca
    22261 aaatgttcgc cgacaatata cgcttccggc tgggtttctt tcgccgcttc ggtgatcccg
    22321 gcaacgtgct gcatattatt gcgcgcccca cccgcctccc ccagcatatg caccacatcc
    22381 agccgccagc cgtccatatt ccacggcgct ttcagccagt ggcggacaat actgtcttcc
    22441 ccgcgataaa tttcattcac cagactttcc gactgataat ccagcttcgt caagctggca
    22501 tagccaagcc agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc
    22561 gattcagagt tgtgacaagc tccccccgtg ccaagattgt gcctgtcaaa ccaggcatgg
    22621 gaatcgccac tgtggttaaa cacgccgtcc agcaccagcc gcattcccag ctgctgcgta
    22681 ttgtgtcgca aacgcagcaa cgccccatca ccgccaaact gcggatcgac atggcgataa
    22741 tcctcggtat cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgct
    22801 gtcacgccaa gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca
    22861 tagaacgttg atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc
    22921 tcttgtccgg ctgcatgatg gtaatagaca tgatcctgtt cagcttcacg aggaagacta
    22981 cgcgcaaaac gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg
    23041 atatcaggta catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc
    23101 tgcggtgtaa accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg
    23161 ggttgtccgc tggagagatc aatcgccgca cgccatgcgg tgacgcctgg ctgcggctga
    23221 ctgcgctgct tatgcattgg tactgacatt tcttcgttat cgtgttctgt acgcagcata
    23281 atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt
    23341 tgtttaacaa atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga
    23401 tgaaaatctg atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt
    23461 tgggggagga ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga
    23521 accggcttac agaacgcaag tattattggt tttcagccag ctgacgatcg tggcggcgtt
    23581 taaacatcca gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac
    23641 gcgtatccgg gtgataacca atcaacccga taataaagag caggaaaatc agcccgccga
    23701 tggtcgttgc taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca
    23761 aacggcgacg gaaggcgatt tgcgacagca ggatcataat ccacacccat accgtggcga
    23821 aggttgccag cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca
    23881 ccgcaaacag cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg
    23941 acgttttgct gaaaattttc ggtgcgctgc cctgctctgc cataccgtgg agcatacggc
    24001 ctacgccaaa tacgtcactg ttaattgccg acagcgaagc ggtcagcaca acaaagttaa
    24061 gaatgctggc ggcaaaggta atgcccatat gctggaacgt cagtacgaac gggctaccgg
    24121 cagtgccaac ctgattccac gggtagatag acataatgac gaacagcgta ccaacgtaga
    24181 acaccagaat acgcatcggc acggagttaa tcgcacgcgg aatcgatttc tcaggatctt
    24241 tcgcttcacc ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt
    24301 gcaacgacat taccatgcca agccagccgt tactgaagaa gccgccgttg ctccacaggt
    24361 tatgaatacc ggtcggttgc ccgccgttgc caatccccca gatgatgatg ccgaaaccgg
    24421 cgacaatcat gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctcac
    24481 cgaagacctt cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc
    24541 aaatccagtg cggcaccgtc gggaacaaga cacccatata gataccaaaa gcggtcacat
    24601 cggcgatggc gacaataagg atttcaaaac agtaggtcca tccggtaatg taacctgcca
    24661 gcgggccaag gttttcctgc gcataacgcg agaaagagct ggcggccggg ttatgtaccg
    24721 acatttcccc cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca
    24781 acaacacgct cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtgc
    24841 caattgctga acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct
    24901 tattcttact ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc
    24961 agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac
    25021 ctgccgcgcg atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg
    25081 ccagaccttg ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg
    25141 cagatgcttt gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa
    25201 tcacgcggga cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac
    25261 acggcggata aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga
    25321 ggttagacac caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg
    25381 gtacgtactg ggcaaagaat tctgcacaag cacaggtcag gccaaccgcc gtgaccagac
    25441 aggcgatgaa gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa
    25501 cgtaagcatg cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg
    25561 aacccagaca gaacagcgcc aggtacagca aagtcagacc aacacccgcc atcaggccag
    25621 cccagacggt ataacgggtc agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt
    25681 taacaataac gataccaaac accattgcgc ccagcgtatc catggtcaga tagccgttaa
    25741 cgaagccgtt agaaaatgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg
    25801 ccggccagac aatcgcgaca acagacagga tgaccagcgc gataattttc agcggcgcaa
    25861 ggaagttgcc cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag
    25921 cgaaatagac caggctgtag ataaacagcg gcagcgcgga atcacccgtc agcggcgcaa
    25981 tccccacttc aaaggaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca
    26041 ggtaacaaac cgttgccagc agtacgccag cgactttacc gattggcgtg ctgaggctgt
    26101 caacaccgcc gccaactttt gccagcgcca ctaccgttaa taccggcagg ccaacggcag
    26161 taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc tgcctgtaag ccgaccattg
    26221 gagggaaaat aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga
    26281 tgatatcgcg cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg
    26341 taacgttaaa tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt
    26401 agcgggaaat actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat
    26461 gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg
    26521 agggatctaa aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta
    26581 tttccgtgaa aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc
    26641 taacgcccgt attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa
    26701 aagatgacaa aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa
    26761 ctgaaacgtg ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc
    26821 accgcatgtt tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct
    26881 ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt
    26941 ccgttatctt caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata
    27001 tgcgtgcctt ccggcgtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt
    27061 agctgatctt cgttgccaga caccttgagg ccgttatcta tctcaaaggt aaatgtctgt
    27121 tttttctgac tcagagtctg agcctcgcgc tcaacaacgc gcagcatcat cggcacatca
    27181 accttttcat tgagcaattg cgtcggcgcg gcttcaattt tcgacagcgt tagcaactgc
    27241 ttcaccagcc cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt
    27301 accgcgcctt ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat
    27361 ggcgtacgta actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga
    27421 tgcatttgcg tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg
    27481 cgaatttcca gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc
    27541 gttttcagat attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta
    27601 tcttccggcc agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg
    27661 ccctcttccg tggtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt
    27721 ttaatcagat tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag
    27781 ccgtatagta gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc
    27841 cacagccacc atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc
    27901 gatgccagca aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg
    27961 cagcaaagta gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat
    28021 actccagtta agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg
    28081 gttgaaaaac gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc
    28141 tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg
    28201 ttagttcccc agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc
    28261 ataaaaaagt gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc
    28321 atcactcggt gagatgtcgg gttgagactt aatccctgca tcttaatcac ctcttccacc
    28381 gccattggcg aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa
    28441 aatggcttgg tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct
    28501 tcccctctgg cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg
    28561 tgtttgatga actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc
    28621 ggccagggtt cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga
    28681 aagccatttt gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg
    28741 accagaatac gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg
    28801 cgacgtcatt atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc
    28861 aggttattga tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg
    28921 cgaccatgct ttttcgccag ggaaccgtta tgcgcatcct tcacacctca gactggcatc
    28981 tcggccagaa cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc
    29041 tgctggagac agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg
    29101 ataccggctc gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac
    29161 agcaaactgg ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga
    29221 atgaatcgcg cgatatcatg gcgttcctca atacaaccgt ggtcgccagc gccggacatg
    29281 cgccgcaaat cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt
    29341 ttttacgccc gcgtgacatt atttccagcc aggcggggct taacggtatt gaaaaacagc
    29401 agcatttact ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac
    29461 tgcgcggcga tcagcctctg cccatcatcg ccacgggaca tttaacgacc gtgggttcca
    29521 gtaaaagtga cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccggcacaaa
    29581 actttccacc agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg
    29641 gcatggaaca tgttcgctat tgcggttccc ccattccact gagttttgat gaatgcggta
    29701 agagtaaata tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc
    29761 tgaacgtacc ggtaactcaa cccatggcgg tgctgaaagg cgatctggcg tcgattaccg
    29821 cacagctgga acagtggcgc gatgtatcgc aggagccacc tgtctggctg gatatcgaaa
    29881 tcactactga tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat
    29941 tgcctgtcga agtattgctg gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca
    30001 gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg
    30061 cactggaaga cctggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt
    30121 tgcgtaccct cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct
    30181 gaactcatta aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg
    30241 gctgtttgcc attaccggcc caaccggtgc ggggaaaacc accctgctgg acgccatttg
    30301 tctggcgctg tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct
    30361 catgacccgc gataccgctg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc
    30421 gtatcgcgcg ttctggagcc agaatcgggc gcgtaaccag ccggacggta atttgcaggt
    30481 gcctcgcgta gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga
    30541 taagctggaa ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat
    30601 gctgctttcg caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga
    30661 attgctcgag gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga
    30721 gcagcacaaa tcggcccgca ctgagctgga gaagctgcaa gcgcaggcca gcggcgtcac
    30781 gttgctcacg ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga
    30841 agaaaaacag ttaattaccg cgcagcagca agaacaacaa tcgctaaact ggttaacgcg
    30901 tcaggacgaa ttgcagcaag aagccagccg ccgtcaacag gccttgcaac aggcgttagc
    30961 cgaagaagaa aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa
    31021 tcttcgtcca cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca
    31081 gcagattgaa gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg
    31141 ccaccacgcg gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg
    31201 gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca
    31261 gttctcccaa caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca
    31321 tgctgagcaa aaacttaatg cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt
    31381 tgctaccgcc ctggcgcaac atgccgagca acgcccactg cgtcagcgcc tggtcgcgct
    31441 gcatgggcag attgttcccc aacaaaaacg tctggcgcag ttaatggtca ctatccagaa
    31501 tgtcactctg gaacagacgc aacgtaatgt cgcacttaac gaaatgcgcc agcgttataa
    31561 agaaaagacg cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa
    31621 aacgctggaa gcccagcgtg cacagttaca ggcgggtcag ccttgtccac tttgtggttc
    31681 caccagccac ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg
    31741 attactggcg ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg
    31801 gcaactggat gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg
    31861 acaagatgag caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac
    31921 cttgcagcca caggacgata ttcaaccgtg gctggatgca caagatgagc acgaacgcca
    31981 gctgcggtta ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca
    32041 aattatccag tatcaacagc aaattgaaca acgtcagcag cagctcttaa cggcattggc
    32101 gggttatgca ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca
    32161 gcaagaagcg cagagctggc agcaacgcca gaacgaatta accgcgctgc aaaaccgtat
    32221 tcagcagctg acaccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga
    32281 agaaactgtg gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag
    32341 ccagcagcag acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca
    32401 agcgcagttt gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc
    32461 ggcgctaatg gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa
    32521 ccagcgccgt caggcgcaaa cgctggtcac tcagacagca gaaacgctgg cacagcatca
    32581 acaacaccga cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt
    32641 agcgcaaact caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca
    32701 gctgaagcag gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca
    32761 aatgacgcag caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg
    32821 cgataaattc cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa
    32881 tcagcaactt acccggctgc acgtgcgcta tctgttacag cgcaaagcca gcgaggcgct
    32941 ggaagtcgag gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc
    33001 cggcggcgaa agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag
    33061 ccataaaaca cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga
    33121 aacgctggat accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg
    33181 tgtgattagc cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa
    33241 gatcaacggc ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag
    33301 caggataatg aatacagagg ggtgaattat ctcttggcct tgctggtcgt tatcctgcaa
    33361 gctatcactt tattggctac ggtgattggt agccgttctg gtggttgtga tggtggtatg
    33421 aaaaaagtca ttttatcttt ggctctgggc acgtttggtt tggggatggc cgaatttggc
    33481 attatgagcg tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg
    33541 catatgatct cgtattatgc actggtggtg gtggtcggtg cgccaatcat cgcactcttt
    33601 tccagccgct actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc
    33661 aacgccatgt tcacgctctc ttcgtcttac ctgatgctcg ccattggtcg gctggtatcc
    33721 ggctttccgc atggcgcatt ttttggcgtc ggagcgatcg tgttatcaaa aattatcaaa
    33781 cccggaaaag tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg
    33841 ctgggcattc cgctgggaac gtatttaagt caggaatgct ggcgttacac ctttttattg
    33901 atcgctgttt ttaatattgc ggtgatggca tcggtctatt tttgggtgcc agatattcgc
    33961 gacgaggcga aaggaaatct gcgcgaacaa tttcactttt tgcgcagccc ggccccgtgg
    34021 ttaattttcg ccgccacgat gtttggcaac gcaggtgtgt ttgcctggtt cagctacgta
    34081 aagccataca tgatgtttat ttccggtttt tcggaaacgg cgatgacctt tattatgatg
    34141 ttagttgggc tagggatggt gctgggaaat atgctaagtg gcaggatttc aggacgttat
    34201 tcaccactgc gcattgcagc agtgactgac tttatcattg tactggcact gctgatgctc
    34261 tttttctgcg gcggcatgaa aacaacgtcg cttatttttg cttttatttg ttgcgcggga
    34321 ttatttgccc tttcagcacc tctgcaaata ttgttactgc aaaatgccaa aggaggagag
    34381 ttattaggtg ccgcaggtgg gcaaatagcg tttaacctcg gtagcgccgt cggcgcatat
    34441 tgcggaggta tgatgctgac gctggggctg gcatataatt acgtggcgct gcctgccgcc
    34501 ctgctttcgt ttgctgcgat gtcatcgttg ctgctgtatg gtcgctataa gcgccagcaa
    34561 gcggcggata gtccggtgct ggcgaaacca ctggggtaga ttactgtcac ggtgcgcgtt
    34621 gcttattgcc ggatgcggcg tgaacgcctt atccgcccta cgcggttctg gcacattttg
    34681 taggcctgat aagacgcggc aagcgtcgca tcaggcatga tgcgccaatt gcctacgttt
    34741 tttactcttg tggccataac cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg
    34801 ccttactcac cggcgtttca cattcgccgc cgaagacaaa ttgtttaatc aactgcccaa
    34861 ccgtttgata taaacggtct acattgctca tcccgccccc caggacaatc acatccggat
    34921 cgagaatatt cacgacatgt gccagcgatt ttgccagccg cagctcgtag cgacgcaatg
    34981 ccagttccgc taccggatcg ctttcttcaa ccaggcggat aatttcactg cctttcagcg
    35041 catgtccgct caaacgacga taatccgtcg cgaatcccgt gcccgaaata aaggtttcaa
    35101 tacaaccttg tttaccgcaa taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt
    35161 ccatccacgg tagcggattg tgtccccact cacctgccgt gccattgccg ccgatatgcg
    35221 tccgcccatt gaatgccacg cccgcgccgc atcccgtgcc gataatcacg gcaaataccg
    35281 tctgcgctcc cgctgccgcg ccatctactg cttctgaaac cgccagacag ttggcatcat
    35341 ttgccagccg cacttcccgc tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt
    35401 tgagccaggt tgaattgaca ttcttcacca caccggtgta gggcgaaatc gaacctggaa
    35461 tgcccatacc taccgttccg cgctgccctg tcgcctgctc cgccatatca accaacgtgg
    35521 cgatcgtttc aatagtctgc cggtaatcat cacgcggcgt gggcagacga tggcggtaca
    35581 actgctcccc tgcatcgccc agtgcaatca cttcagtttt ggtgccgcct aaatcgatac
    35641 ctatacgcac tgtactctcc ttattttttt caatatcaat agcgtagaga cggacaaccg
    35701 gattggcaat gcaaggccgc cgacaattcg ttatcatgcc cgctaaattt aacgacaagg
    35761 ccgtggaaat tatcatgctg tggttcaaaa atttaatggt ttaccgtctt agccgcgaga
    35821 tttcgctgcg tgcagaagag atggaaaaac agctagcctc gatggcattt accccatgcg
    35881 gcagccagga catggcgaag atgggctggg ttcctccgat gggatcgcac agcgatgcgt
    35941 taacgcacgt tgccaatggt caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc
    36001 cgtctccggt gattaaacag gcgctggaag cgaaaatcgc caaactggaa gcggaacagg
    36061 cgcgtaagct gaagaaaacc gaaaaagatt cgctgaaaga cgaagtgctg cactctctgc
    36121 tgccgcgtgc tttcagccgt tttagccaga caatgatgtg gctcgacacg gttaacggtt
    36181 tgattatggt ggactgcgcc agtgccaaaa aagcggaaga tacgctggca ttactgcgta
    36241 aaagcctggg gtcgttaccg gttgtaccat tgagcatgga aaacccgatt gaactgacgc
    36301 tgaccgaatg ggttcgctcc ggtagtgcgg cacagggctt ccagctgctt gatgaagccg
    36361 agctgaaatc gttgctggaa gatggcggcg tgatccgcgc gaagaaacaa gatctgacca
    36421 gcgaagagat caccaatcac attgaagccg gaaaagtggt gactaaactg gcgctcgact
    36481 ggcagcagcg cattcagttt gtgatgtgcg acgatggttc gctcaagcgt cttaagttct
    36541 gcgacgagct gcgcgatcaa aacgaagata tcgaccgtga agatttcgcc cagcgttttg
    36601 atgccgattt catcctgatg actggtgaac tggcagcgtt aattcaaaac ctgattgaag
    36661 gattaggtgg cgaagcacaa cgttaattgc tgattttcct ttaatgccgg atgcgacgcc
    36721 tgccgcgtct tatccggcgt acgaagccac accaggcata taattattcg ctacggcgag
    36781 caataatttt tagcgcagca atattatgcg ttttacgccg taactgatct aatcgcctca
    36841 tccctattcg cctgtcagca gcacgcagaa tattcactaa cccccattta tcgctggtaa
    36901 attgttttgt taacagctcc gcttttgggg tatcaatata ttcacgcaat aaatcagaaa
    36961 ttgaacaaat atccgtcagg taaggatacc agcaagtttc accgtgaaaa ttacgcactc
    37021 cagcgttacc tgcaataaag tctttcgggt aatcccagat cacgttttta tccaacgtga
    37081 tccaataacg cggtaaatcg gtacttccac cattttggct gcgcatggga taacgggtac
    37141 agtgaatttg caggttgatc gttggcgtca gtaagtcata gatctcccgc tgtaacttgc
    37201 tccatggacg ttgtgtcatt gtttttcctc aagccggtga cgcgcttcac tgcgaatttt
    37261 ccgcgttgcc ccggttttca ctaaatcacg taactgcgaa agatcgggtt gttcgacaaa
    37321 taagagtaat gatggatcct cttttaacca cgccgttttc acatcggcag ccagctgtgg
    37381 attattaata gccagcgacc gatcctgagg ctcagtcaat gtcgttaaca acgacgccgg
    37441 ggtatgtgcg ttgcgtaatg ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc
    37501 acgccaccat tcatcatctg atttatcttt tatcgtagcc agttccgcaa aaaatacctg
    37561 gagatcttca ctgatattta tatcaacctt gatgaattat gaatatatcg gaaattgaaa
    37621 aaatcgcagc aaaggaaata ttattatggg ttaaagagag gatatttcag aaaaatgccc
    37681 tcccgttcaa ggggaaggcg aggaattaca gatagcggca cagataagag gtaggttcgg
    37741 caacttgaag atgaaactca ctgtgaccgg gaacattaaa caccgaaccg gcttcataca
    37801 cttgccagtc ggtcgcgtca ggcagtaaca cattcagcgc gccactgatt accgtcatct
    37861 cttccggctc agcggtgctg aaggtgtatt cgccttcaac cataacaccc acgctggcgc
    37921 gaccagtgct gctgctggaa aagccgatcg atttcacttt gccggaaaag tactcattac
    37981 tttgaagcat aaactggccc tgtctggtag ttgaacatct acttcactat aggggccaga
    38041 ggcgctggct gtcacgcaaa attacaccag taattccgca gccagccgtg caatcaatac
    38101 gttagagagc aagacaggaa catcgagctg tttttgcagt aaatcgcgat gacgttggtg
    38161 aaatcctaaa caatccagca tgatgacatc agcccctttt gccagtaatt ctttcccagc
    38221 atcaatgatt ttttgttctg aatcatgaat ggggttaccc aatgaaaata ccggcgattt
    38281 ctgcaaaatt tgccattttt gcgcctgaac gggcagcatc tcctcaaccg gaacgataac
    38341 ccccacctga tgatcttcaa caatagagga aaccagtgga ggcaatattc gcgacggctc
    38401 aagaaagatc gtattacgcg cagtcatact actaatgttt gctgtactca ttaataaaat
    38461 gacgtcataa ccctgattat cgagcacttc aaccacacct tgcagatctc gctccacttt
    38521 acgacgcgaa acatgggcca gctggttgtc atttaataat gtgagaatgg tgtcttcgcc
    38581 tgcttctggc gcgtactccg ccatcacttc ttcacgactt aacttcccca gcaggctatg
    38641 atgggaaata ttatcttcgt caatgtattc cgtcaggagc ggcaaaacct cctgcatagg
    38701 tacaatgccg atggtcagga tcgccaacga cgcactcatt gttcatccac cttacttttc
    38761 tttcgtcgtt aattaccggg caagtgtgaa gcaccactgc tgacacttac ttgtgctaca
    38821 aatgacaaaa agcgtagcag cagacgtgca cggcataatg taaagattcc aaatgattcc
    38881 agtaatggaa tgttattgtt taatattcta attattagaa aaacataaat tatgaaaaat
    38941 gtgacgcaga tcccttgtag tggcactcag aatcccttcc gagcagtctg atgcggtcac
    39001 tctttgtcag ggtcctcata gcgttttttc gcatccatcg cttcctgagc cgtcggatgt
    39061 tcactgatca acgagtctgg tttaggatga tcggctctga gttggtacca ggttaccgtc
    39121 tgtcctggct ttcctttttc aatcgtcact atatatgctt cacgaggata aggcggtttc
    39181 gttggcatcg ttcttcctta tttcacggga tgaacgttaa gtataggcgc tctaaaatca
    39241 acaattgatc gtctgtgcca gggcgctacg aatttcagaa atcacctggc tgggttcgtt
    39301 tgttgcgtcg atgataatat gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac
    39361 ttcctgaact tcttcgctca gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg
    39421 tgcagcttgc agtcggttaa ccaggactga tactggcgca cacaaataaa ccacgatccc
    39481 gttattttgc atgaagtgac gattaaattc cgtcagaata atgccgccgc ctgtagcgat
    39541 aacggtggat gccgcagtta ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc
    39601 ccactcttcc ctttcgacga tctccgcgac cgtcatattg agctgtgatt gcaaccactg
    39661 atcggtatcg acaaaccgac ggttaagcga atcggcaagg gccattccga ccgttgtttt
    39721 accacagccc cgaggcccga tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata
    39781 ggtcgcaatg cggcgaaagc cggtgtcatg agaatagcga tcataccatc aaactagtac
    39841 aatttcgatt gtaaagaaaa aattccactt aaagtaaaaa tctcaataca tcccttacta
    39901 taccaataaa tattcaagaa tgaagtgtaa ataataaatt acatttagcc acgactacgt
    39961 tgcacttcca gccaccactt ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa
    40021 aggctatctg gtccgccggt ctggatccca ctggcacgta aggtatccat aaaatcgcgc
    40081 atcgtcaggc gctcacgaat ggtcgctggc gtgtaacgtt cgccgcgcga attaagcgcc
    40141 gctgcgcctt tctcgatggc ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg
    40201 cccgcttcac actgccggac aatttcgtta tcggcaacat cgaaacctgc cgcgacgcgc
    40261 agcgtacgaa taaatcgcga tggcggcacg cgtaaactct ggtttgctac cagtaccagc
    40321 ggcatctgca tacgttccgc cgcgcgatac aaaatctctt taattacatt gggacacgcg
    40381 tcggcatcca cccaaattgt cataaagtca tcctttgtta ggtaatcctc tattgtgtcg
    40441 cgcttttgcc ttccggcata gttctgttta tgcttctgcc agcgattatc aaaacaatga
    40501 atttcacggc aggagtgagg caatggaaaa gaaaatcggt tttattggct gcggcaatat
    40561 gggaaaagcc attctcggcg gtctgattgc cagcggtcag gtgcttccag ggcaaatctg
    40621 ggtatacacc ccctccccgg ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc
    40681 cgcagaatcg gcgcaagaag tggcgcaaat cgccgacatc atttttgctg ccgttaaacc
    40741 tggcatcatg attaaagtgc ttagcgaaat cacctccagc ctgaataaag actctctggt
    40801 cgtttctatt gctgcaggtg tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg
    40861 gaaaattatc cgcgccatgc cgaacactcc cgcgctggtt aatgccggga tgacctccgt
    40921 aacgccaaac gcgctggtaa ccccagaaga taccgctgat gtgctgaata ttttccgctg
    40981 ctttggcgaa gcggaagtaa ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg
    41041 ttcttcgcca gcctacgtat ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg
    41101 cgggatgcca cgcgcccagg cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa
    41161 aatggtgctg gaaacgggag aacatccggg ggcactgaaa gatatggtct gctcaccggg
    41221 aggcaccacc attgaagcgg tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat
    41281 cgaagcgatg acgaagtgta tggaaaaatc agaaaaactc agcaaatcct gatgactttc
    41341 gccggacgtc aggccgccac ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc
    41401 gccaaatctg ccgatttcaa ccactcacga tagtgactca tttgtgggtt cagcggcgca
    41461 acccccacac taatccgtaa agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc
    41521 ccttcacgca cccgtaacat ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt
    41581 actgcaaact catcgccgcc aaaccgacca atcacatcgc taccgcgcag ggtaatttgt
    41641 aactgtcggg taagcgccac aatcgcttca tcgcccacat catgacccca ggtatcgttg
    41701 atgctcttga aatggtcgat atcgataatc agtaacgttg catcgcgatt atgccgccga
    41761 cagttatcaa attcattgcg taacatagtt tcccaatgac gtcggttata cacgcccgtc
    41821 atgccgtcgc gagtactcat gacctgcaac ctgcgtttat gttccgccag tttggttgcc
    41881 gtctggtagc tgacccagcc aaacatcaga ggataaacga caataatggg aagggagagc
    41941 caccattcca gcggagcact attgaacgac acggtaatgc ccgtaagctc gagggtgaca
    42001 aggcaggaaa ccaccatcaa caccagaccc gcgacaaaca gacgggggcc gcctgccccc
    42061 atcaaattca gacacagaat catcaacata gcggtggaag gcagcacgtt tacgcccatt
    42121 acgcctaccc acattcccgc taatactgca tcggttttta agttgtaaat ttcccggcta
    42181 agcggatcga cggccctgct cgctatctgc caggctaaat gcggccagac gaacgcccag
    42241 ccgaccaaca ccagccacca ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc
    42301 ggtaagaaca tgccagccag gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc
    42361 agcccggacc gctgatgttc gttttgagga gttaaaggag gttcctcccc atgcgccgcc
    42421 gcttttttga aaaagttttc atcattcatt atttttggga acattcagaa acaattttcc
    42481 caaattatag agacggggct aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta
    42541 gctcacccgt tagcacta
//
LOCUS       DAZPFP010000017        40880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPFP010000017 DAZPFP010000000
VERSION     DAZPFP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.017"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUR4_000862"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUR4_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1454557.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUR4_000863"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUR4_000863"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1454558.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUR4_000864"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUR4_000864"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1454559.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUR4_000865"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUR4_000865"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1454560.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUR4_000866"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUR4_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1454561.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHUR4_000867"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHUR4_000867"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1454562.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUR4_000868"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUR4_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1454563.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUR4_000869"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUR4_000869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1454564.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHUR4_000870"
     CDS             complement(8942..9223)
                     /locus_tag="ACHUR4_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454565.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHUR4_000871"
     CDS             complement(9237..10898)
                     /locus_tag="ACHUR4_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1454566.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHUR4_000872"
     CDS             complement(10882..11208)
                     /locus_tag="ACHUR4_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1454567.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHUR4_000873"
     CDS             complement(11264..11446)
                     /locus_tag="ACHUR4_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454568.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHUR4_000874"
     CDS             complement(11430..11870)
                     /locus_tag="ACHUR4_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1454569.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHUR4_000875"
     CDS             complement(11870..12166)
                     /locus_tag="ACHUR4_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR1454570.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHUR4_000876"
     CDS             complement(12163..12501)
                     /locus_tag="ACHUR4_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR1454571.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHUR4_000877"
     CDS             complement(12498..13709)
                     /locus_tag="ACHUR4_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1454572.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHUR4_000878"
     CDS             complement(13711..14283)
                     /locus_tag="ACHUR4_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR1454573.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHUR4_000879"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHUR4_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACHUR4_000880"
     CDS             complement(15781..16005)
                     /locus_tag="ACHUR4_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454574.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACHUR4_000881"
     CDS             complement(16046..16159)
                     /locus_tag="ACHUR4_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1454575.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACHUR4_000882"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACHUR4_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACHUR4_000883"
     CDS             complement(16318..16623)
                     /locus_tag="ACHUR4_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454576.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACHUR4_000884"
     CDS             complement(16620..16898)
                     /locus_tag="ACHUR4_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454577.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17178..18926)
                     /locus_tag="ACHUR4_000885"
     CDS             complement(17178..18926)
                     /locus_tag="ACHUR4_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR1454578.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18923..19222)
                     /locus_tag="ACHUR4_000886"
     CDS             complement(18923..19222)
                     /locus_tag="ACHUR4_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1454579.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19228..19461)
                     /locus_tag="ACHUR4_000887"
     CDS             complement(19228..19461)
                     /locus_tag="ACHUR4_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454580.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19463..19683)
                     /locus_tag="ACHUR4_000888"
                     /pseudo
     CDS             complement(19463..19683)
                     /locus_tag="ACHUR4_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19676..19897)
                     /locus_tag="ACHUR4_000889"
     CDS             complement(19676..19897)
                     /locus_tag="ACHUR4_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454581.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19887..20087)
                     /locus_tag="ACHUR4_000890"
     CDS             complement(19887..20087)
                     /locus_tag="ACHUR4_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1454582.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20217..20396
                     /locus_tag="ACHUR4_000891"
     CDS             20217..20396
                     /locus_tag="ACHUR4_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454583.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20521..20850
                     /locus_tag="ACHUR4_000892"
     CDS             20521..20850
                     /locus_tag="ACHUR4_000892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454584.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20900..21088)
                     /locus_tag="ACHUR4_000893"
     CDS             complement(20900..21088)
                     /locus_tag="ACHUR4_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1454585.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21463..22692)
                     /locus_tag="ACHUR4_000894"
     CDS             complement(21463..22692)
                     /locus_tag="ACHUR4_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1454586.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUR4_000895"
                     /gene_synonym="ycfD"
     CDS             22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUR4_000895"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1454587.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUR4_000896"
     CDS             complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUR4_000896"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1454588.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25587..26723
                     /gene="potA"
                     /locus_tag="ACHUR4_000897"
     CDS             25587..26723
                     /gene="potA"
                     /locus_tag="ACHUR4_000897"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1454589.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26707..27564
                     /gene="potB"
                     /locus_tag="ACHUR4_000898"
     CDS             26707..27564
                     /gene="potB"
                     /locus_tag="ACHUR4_000898"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1454590.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27561..28355
                     /gene="potC"
                     /locus_tag="ACHUR4_000899"
     CDS             27561..28355
                     /gene="potC"
                     /locus_tag="ACHUR4_000899"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1454591.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28352..29398
                     /gene="potD"
                     /locus_tag="ACHUR4_000900"
     CDS             28352..29398
                     /gene="potD"
                     /locus_tag="ACHUR4_000900"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1454592.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUR4_000901"
     CDS             complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUR4_000901"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1454593.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUR4_000902"
     CDS             complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUR4_000902"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1454594.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUR4_000903"
     CDS             complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUR4_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1454595.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUR4_000904"
     CDS             complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUR4_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1454596.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUR4_000905"
     CDS             complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUR4_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1454597.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34730..35803
                     /locus_tag="ACHUR4_000906"
     CDS             34730..35803
                     /locus_tag="ACHUR4_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1454598.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUR4_000907"
     CDS             35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUR4_000907"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1454599.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUR4_000908"
     CDS             39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUR4_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR1454600.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40565..40777)
                     /locus_tag="ACHUR4_000909"
     CDS             complement(40565..40777)
                     /locus_tag="ACHUR4_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR1454601.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9617 a        10793 c        10100 g        10370 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat tgcgttagtc
    17221 tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt cgcggctggc
    17281 ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc gtacgccata
    17341 aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga atcgccaccg
    17401 ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca aaactcaata
    17461 accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg ttccagtagt
    17521 aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat aatgacaggg
    17581 atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact aacgatattg
    17641 tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt gaatatcatc
    17701 gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg cttttcgtat
    17761 ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt tccttcgcct
    17821 gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg cgcacgtcca
    17881 ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc tgccagaagg
    17941 ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc ctcaataaac
    18001 atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat acgcatcatc
    18061 ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag cgcgttatcg
    18121 cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt ggtgatccag
    18181 ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc attggcgaac
    18241 ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa cgcttccacg
    18301 gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat caccaccatt
    18361 tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca tacgtgaacg
    18421 atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag ctgcgcgcgc
    18481 tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt aagccctgcg
    18541 gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt ctggacgtgc
    18601 attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt ctgaccaatc
    18661 tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg tttttttcca
    18721 tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag ccacacaggc
    18781 ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc gtgcgaccag
    18841 gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc gcgtggctgt
    18901 ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc atctttttta
    18961 tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg ggcgctgcct
    19021 gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca tattcgtaac
    19081 cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc atgccgccgt
    19141 actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca ctggcagtta
    19201 agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt tatatctgtc
    19261 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
    19321 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
    19381 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
    19441 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
    19501 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
    19561 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
    19621 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
    19681 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
    19741 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
    19801 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
    19861 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
    19921 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
    19981 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
    20041 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
    20101 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
    20161 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
    20221 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
    20281 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
    20341 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
    20401 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
    20461 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
    20521 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
    20581 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
    20641 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    20701 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    20761 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
    20821 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    20881 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    20941 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    21001 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
    21061 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
    21121 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
    21181 gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
    21241 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
    21301 cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa tctgtacact
    21361 ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg ttgtatctgt
    21421 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    21481 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    21541 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    21601 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    21661 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    21721 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    21781 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    21841 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    21901 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    21961 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    22021 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gtttttcccc
    22081 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    22141 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    22201 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    22261 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    22321 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    22381 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    22441 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    22501 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    22561 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    22621 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    22681 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    22741 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    22801 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    22861 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    22921 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    22981 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    23041 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    23101 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    23161 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    23221 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    23281 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    23341 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    23401 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    23461 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    23521 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    23581 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    23641 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    23701 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    23761 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    23821 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    23881 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    23941 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    24001 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    24061 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    24121 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    24181 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    24241 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
    24301 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    24361 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    24421 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    24481 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    24541 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    24601 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    24661 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
    24721 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    24781 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    24841 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    24901 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
    24961 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    25021 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    25081 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    25141 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    25201 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    25261 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    25321 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    25381 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    25441 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    25501 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
    25561 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    25621 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    25681 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    25741 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    25801 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    25861 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    25921 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    25981 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    26041 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
    26101 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    26161 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    26221 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    26281 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    26341 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    26401 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    26461 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    26521 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    26581 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    26641 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    26701 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    26761 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    26821 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
    26881 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    26941 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    27001 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    27061 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    27121 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    27181 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    27241 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    27301 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    27361 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    27421 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    27481 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    27541 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    27601 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    27661 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    27721 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    27781 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    27841 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    27901 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    27961 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    28021 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    28081 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    28141 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    28201 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    28261 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    28321 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    28381 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    28441 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    28501 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    28561 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    28621 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    28681 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    28741 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
    28801 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    28861 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    28921 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    28981 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    29041 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    29101 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    29161 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    29221 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    29281 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    29341 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    29401 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    29461 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    29521 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    29581 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    29641 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    29701 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    29761 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    29821 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
    29881 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    29941 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    30001 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    30061 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    30121 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    30181 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    30241 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    30301 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    30361 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    30421 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
    30481 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    30541 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    30601 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    30661 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    30721 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    30781 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    30841 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    30901 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
    30961 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    31021 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    31081 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    31141 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    31201 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    31261 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    31321 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    31381 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    31441 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
    31501 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    31561 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    31621 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    31681 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    31741 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    31801 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    31861 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    31921 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    31981 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    32041 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    32101 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    32161 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    32221 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    32281 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    32341 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    32401 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    32461 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    32521 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    32581 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    32641 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    32701 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    32761 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    32821 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    32881 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    32941 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    33001 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    33061 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    33121 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    33181 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    33241 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    33301 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    33361 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    33421 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    33481 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    33541 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    33601 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    33661 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    33721 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    33781 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    33841 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    33901 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    33961 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    34021 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    34081 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    34141 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    34201 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    34261 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    34321 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    34381 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    34441 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    34501 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    34561 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    34621 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    34681 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    34741 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    34801 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    34861 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    34921 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    34981 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    35041 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    35101 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    35161 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    35221 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    35281 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    35341 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    35401 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    35461 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    35521 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    35581 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    35641 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    35701 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    35761 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    35821 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    35881 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    35941 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    36001 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    36061 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    36121 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    36181 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    36241 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    36301 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    36361 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    36421 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    36481 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    36541 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    36601 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    36661 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    36721 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    36781 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    36841 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    36901 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    36961 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    37021 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    37081 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    37141 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    37201 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    37261 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    37321 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    37381 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    37441 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    37501 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    37561 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    37621 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    37681 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    37741 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    37801 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    37861 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    37921 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    37981 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    38041 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    38101 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    38161 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    38221 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    38281 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    38341 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    38401 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    38461 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    38521 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    38581 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    38641 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    38701 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    38761 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    38821 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    38881 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    38941 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    39001 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    39061 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    39121 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    39181 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    39241 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    39301 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    39361 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    39421 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    39481 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    39541 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    39601 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    39661 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    39721 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    39781 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    39841 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    39901 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    39961 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    40021 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    40081 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    40141 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    40201 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    40261 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    40321 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    40381 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    40441 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    40501 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    40561 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    40621 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    40681 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    40741 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    40801 tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagaaat
    40861 tacttaacga tcgataacta
//
LOCUS       DAZPFP010000018        39979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPFP010000018 DAZPFP010000000
VERSION     DAZPFP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.018"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..156
                     /locus_tag="ACHUR4_000910"
     CDS             <1..156
                     /locus_tag="ACHUR4_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1454602.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="ACHUR4_000911"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="ACHUR4_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1454603.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3066
                     /gene="uidA"
                     /locus_tag="ACHUR4_000912"
     CDS             1363..3066
                     /gene="uidA"
                     /locus_tag="ACHUR4_000912"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1454604.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="ACHUR4_000913"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="ACHUR4_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="ACHUR4_000914"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="ACHUR4_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1454605.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="ACHUR4_000915"
     CDS             complement(5627..7135)
                     /locus_tag="ACHUR4_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1454606.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACHUR4_000916"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACHUR4_000916"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1454607.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="ACHUR4_000917"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="ACHUR4_000917"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1454608.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="ACHUR4_000918"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="ACHUR4_000918"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1454609.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACHUR4_000919"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACHUR4_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1454610.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACHUR4_000920"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACHUR4_000920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1454611.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="ACHUR4_000921"
     CDS             complement(14284..15603)
                     /locus_tag="ACHUR4_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR1454612.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACHUR4_000922"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACHUR4_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1454613.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="ACHUR4_000923"
     CDS             16700..17035
                     /locus_tag="ACHUR4_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1454614.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACHUR4_000924"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACHUR4_000924"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1454615.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="ACHUR4_000925"
     CDS             complement(17791..19173)
                     /locus_tag="ACHUR4_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1454616.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACHUR4_000926"
     CDS             complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACHUR4_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1454617.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="ACHUR4_000927"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="ACHUR4_000927"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1454618.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="ACHUR4_000928"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="ACHUR4_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1454619.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACHUR4_000929"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACHUR4_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1454620.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACHUR4_000930"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACHUR4_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1454621.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="ACHUR4_000931"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="ACHUR4_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1454622.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="ACHUR4_000932"
     CDS             complement(25948..26769)
                     /locus_tag="ACHUR4_000932"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1454623.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="ACHUR4_000933"
     CDS             complement(26869..26952)
                     /locus_tag="ACHUR4_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454624.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACHUR4_000934"
     CDS             complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACHUR4_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1454625.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27624..27728
                     /locus_tag="ACHUR4_000935"
     CDS             27624..27728
                     /locus_tag="ACHUR4_000935"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1454626.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27777..29030)
                     /locus_tag="ACHUR4_000936"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="ACHUR4_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29137..30039
                     /locus_tag="ACHUR4_000937"
     CDS             29137..30039
                     /locus_tag="ACHUR4_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1454627.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30174..31394
                     /gene="mlc"
                     /locus_tag="ACHUR4_000938"
                     /gene_synonym="dgsA"
     CDS             30174..31394
                     /gene="mlc"
                     /locus_tag="ACHUR4_000938"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1454628.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31519..32214
                     /gene="bioD"
                     /locus_tag="ACHUR4_000939"
     CDS             31519..32214
                     /gene="bioD"
                     /locus_tag="ACHUR4_000939"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1454629.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32167..33459)
                     /gene="clcB"
                     /locus_tag="ACHUR4_000940"
     CDS             complement(32167..33459)
                     /gene="clcB"
                     /locus_tag="ACHUR4_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1454630.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33618..34232)
                     /gene="dmsD"
                     /locus_tag="ACHUR4_000941"
     CDS             complement(33618..34232)
                     /gene="dmsD"
                     /locus_tag="ACHUR4_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1454631.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34275..35129)
                     /gene="ynfH"
                     /locus_tag="ACHUR4_000942"
     CDS             complement(34275..35129)
                     /gene="ynfH"
                     /locus_tag="ACHUR4_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1454632.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35131..35748)
                     /gene="ynfG"
                     /locus_tag="ACHUR4_000943"
     CDS             complement(35131..35748)
                     /gene="ynfG"
                     /locus_tag="ACHUR4_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1454633.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35759..37797)
                     /locus_tag="ACHUR4_000944"
                     /pseudo
     CDS             complement(35759..37797)
                     /locus_tag="ACHUR4_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37858..38892)
                     /locus_tag="ACHUR4_000945"
                     /pseudo
     CDS             complement(37858..38892)
                     /locus_tag="ACHUR4_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38950..>39979
                     /locus_tag="ACHUR4_000946"
     CDS             38950..>39979
                     /locus_tag="ACHUR4_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1454634.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10164 a         9697 c        10237 g         9881 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa cactctcgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggtat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgcccta
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgtca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccgcgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgcgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    29101 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    29161 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    29221 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    29281 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    29341 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    29401 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    29461 gtgtccgata cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc
    29521 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    29581 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    29641 atggcgatga tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa
    29701 ctggctgaac tggcgaaaga accctttgtc ttttttgatc cgcacgtcgg gactgggctg
    29761 tatgacgata ttctcggact gatgcgacgt taccatttga cgcccgtcat cactcaggag
    29821 gtgggcgagg caatgaccat catcggtctg gtttccgccg gtctgggtgt ttcaattttg
    29881 cctgcgtcat ttaaacgtgt tcagctcaac gaaatgcgct gggtgccgat tgctgaagag
    29941 gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag tccagctgcg
    30001 cgtaactttc gtattcatct gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc
    30061 ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt tgacgacacg tattgaagtg
    30121 cttcaccata gcctacagat tatttcggag cgcgaaaata tagggagtat gcggtggttg
    30181 ctgaaaacca gcctgggcac attgatcaaa taaagcagac caacgcgggc gcggtttatc
    30241 gcctgattga tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg gcgcagctgg
    30301 ctcctgccag tatcactaaa attgtccgtg agatgctcga agcacacctg gtgcgagagc
    30361 tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt ggggctggtg gttgaaactg
    30421 aagcctggca ctatctttct ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg
    30481 atctgagcag caaactggtg gtggaagagt cgcaggaact ggcgttaaaa gatgacttgc
    30541 cattgctgga tcgtatcatt tcccatatcg atcagttttt tatccgccac cagaaaaaac
    30601 ttgagcgtct aacttcgatt gccataaccc tgccgggaat tattgatacg gaaaatggta
    30661 ttgtacatcg catgccgttc tacgaggatg taaaagagat gccgctcggc gaggcgctgg
    30721 agcagcatac cggcgtaccg gtttatattc agcatgatat cagcgcatgg acgatggcag
    30781 aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca
    30841 acgtgggggt gggcgtcatt accgatggtc atctgctaca cgcaggcagc agtagtctcg
    30901 tggaaatagg ccacacacag gtcgacccat atggtaaacg ctgttattgc gggaaccacg
    30961 gctgcctcga aaccatcgcc agtgtggaca gtattcttga gctggcacag ctgcgtctca
    31021 atcaatccat gagctcgatg ttacatgggc aaccgttaac cgtggactca ttgtgtcagg
    31081 cggcattgcg cggcgatcta ctggcaaaag acatcattat cggggtgggc gcgcatgtag
    31141 ggcgcattct tgccatcatg gtgaatttat ttaacccaca aaaaatactg attggctcac
    31201 cgttaagtaa agcggcagat atcctcttcc cggtcatctc agacagcatc cgtcagcagg
    31261 cccttcctgc gtatagtcag cacattagcg ttgagagtac tcagttttct aaccagggca
    31321 cgatggcagg cgctgcactg gtaaaagacg cgatgtataa cggttctttg ttgattcgtc
    31381 tgttgcaggg ttaacatttt ttaactgttc taccaaaatt tgcgctatct caatttgggc
    31441 caggaaagca taacttagac tttcaaggtt aattattttc ctagtttata tttgtgaagc
    31501 ataacggtgg agttagtgat gctgaagcgt ttctttatta ccggtacaga cacttctgta
    31561 gggaaaacgg tggtttcccg cgcattgcta caagcgttag cctcccaggg aaaaagggtt
    31621 gcgggatata aacccgtagc gaaggggagc aaagagacac ccgaagggct gcgtaataaa
    31681 gatgccttgg tgttgcagag tgtttcaacc atcgaactgc cttatgaagc agttaatcct
    31741 atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc
    31801 atttcaaacg gcctggcaaa cctgaccgat aaagtcgatc atgtcgtggt agaagggact
    31861 ggcggctggc gcagtctgat gaatgatttg cgtccactct ctgaatgggt agtgcaggaa
    31921 caactgccgg tgttgatggt tgtcggtatt caggaaggtt gcattaacca tgcactgcta
    31981 acagctcagg cgatcgccaa cgacgggctg ccgctcattg gctgggtggc taaccgaatc
    32041 aacccaggac tggcgcatta tgcggaaatc attgatgtgc tgggtaaaaa acttccggca
    32101 ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac agcgtgaact ggggcaatac
    32161 atccgcttag ctatgctgcg cagtgtgctg gcggtagata gagtcacggt gtaacgtccg
    32221 cgaaattacc gacgcaatta cgcaggcaat caataaaccg gggagtagct gatactcccc
    32281 ggtcatttca catatcatca acgtcgacat aatcggcgcg tgcgtggttg ccgccaacag
    32341 tgtcgccatc ccggtcaatc cgagtaaaag cgtaatttct tcgccatcag ggaaccataa
    32401 tcccaggcta cgaccataca acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa
    32461 gacaccaccg ggtgcgccgg aaccgctact cgccagcacg gcacacagtt tacaaaggaa
    32521 gatcccggca atgatcatta acagtggtgg ggcggttaaa aaggattgta cggtgctata
    32581 gccgttgccc cacactgcag gtgtgaacag ggaaagcaga cccacgatca acccgcctag
    32641 tgccagttgc cagggcggcg caagtttgag actcacaaat ccacgatgac aggcgttcat
    32701 taacgttaac aacagtggtc cgcacagacc tgccagcaca cctgtactga taatcagcgc
    32761 atagtcacga gcctgaaccg tcactgagag ttgtacgttg tagagtaacg cgtcgctatg
    32821 attaatcaga ttgctaacca gccatgccac gatggcggaa ataatcaccg ggccgagaga
    32881 ggccaacatc atagtgccaa acagcacttc ggctataaat aaactgccag caagcggggc
    32941 acgataggcc gcagccattc ccgccgcggc cccacaggcg atccataatt tccattcctg
    33001 gcgtggcgta aaacgttggg caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc
    33061 accttcgcga ccaattgcac tgccgctggt tactaccagc agcgaggcaa gcgatttaac
    33121 caggcttgct gcgtaatcga actgtccatc ggtttgcaac gcttccatgt aatcggtcgg
    33181 cgcatgaggg cgttgttggg taaatttctg ccagcccatc agcaacaaac ccgccgccag
    33241 tccgccgagc gccggagtta gcagccgtcg ccaggaggaa aggtttgtcg ctgcattgac
    33301 cagactgccg gagtcattat tgaggaacaa ccactccagt agcagcatcg catgacgaaa
    33361 cccggcaacg gcaaagacag cgagaatacc gacgactgtt gcgataagca gacggcggaa
    33421 catagtgcgc agatccggat agatgtgtag atgatgcatg agcagaaatg ggcaatgaaa
    33481 cacgggcgac tattttgcgg caaaagaggc caaccgcaaa agcagtgcgt gctttaagag
    33541 ataaaaaaac gtgacattgt aacgtattat tgccgaatcg tgcgtaatcg tcaccatccg
    33601 gcaatattac ggtgatccta tcgaaagagt ggtttaaccg cgacaggaat caacagttgt
    33661 gattgccatt gcgccagcgt taagcgagcc agttcaccca gtgcacggta gaaagggtgt
    33721 tctgcttttt cgataaaaac atcaagaaaa cgtgttgacc acggaaaaag gtgccatgcc
    33781 agcaattctt cgcattctgt ctggcgacca ttctccgcca accacgcagc catcaacagc
    33841 agtgaaccaa aatgatcttc cggttcgttt tgcttcattt caaactgaat gcctttctcg
    33901 cgcatccact gacgaagtgc caatgttgaa tcgccaaaca gcacagattc gcgatccagc
    33961 cagaccgacc cccacggcgg agacggcagt gcccacgggc cgacaaacaa acgctgccag
    34021 gcctgggcgt gagtctcttc acactgtgtc tgaaaagccg tcaccagcgg cgctaatgac
    34081 gcttctggta gaggccactg agtttcccag ccgtcactgg tgagtaccgc aaccagaggt
    34141 gcggcttccg cactctctgg agcgtaataa aacagcgcgc ccaacacgcg cgccgcgaca
    34201 gaaaaattat cttgctgtga aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc
    34261 cccgcaccaa tttgttaacc tgcaatagcc attcctactg tcatgtgcag gccataaaag
    34321 agcacacgcc cgatcatctc accaacaagt atgaggatta gccccagaac aagtccggca
    34381 acgtgaggtt cacggcgacg aatgagcgga caaagccagc atccaagacc tgcgcataac
    34441 aataccacac gccagacctg taaggaggcg taatctggta ccagtgcgct ggcttgttgc
    34501 acggatgagt gaatagacgc cagagacaaa ccttgcagca caatcacctc ggcacaagcg
    34561 atcagcgcca gaacgctaat gatggcaaat ggtgtggtat taaatgtaac gcgggctgcc
    34621 cgcagaattg cggcagcaag tattggaccg ctcaacaata ccgtcaggaa gaaagccagc
    34681 gtagtgtaac cgttatgcca ggttggcacg gtgtcgattt gatacacgca ggtcatcatc
    34741 cagacgaaaa tgacgccgag cgccatgctg acaagtaacc agagtttccc caacgcttgc
    34801 ggcattttac cgatgaccgc caccagccac catagtccgc caacggcaaa aaatatggaa
    34861 ccggcagcaa tctcgttact cagaccagaa gctccgattc gattcagcga gttaaacgct
    34921 cgcagtggcg atcccaggtg catgacagag gcaataaacc cgatgcccat caacagccag
    34981 aggaaaaaca tgccacgaac aatgtgctga cggtctgcgt catttttggc ggcgaaccag
    35041 ccaattccgc taacaatcag cgcgcctact acgcattgac ccaatacggt aaagataacc
    35101 agtggccatt catgccatcc atttcccatt ttacacttcc tccggattag ccagataacc
    35161 agtggtatcg ccggtcgggc ggctgttggc gttaggtttg ataacgatat tgggttttgt
    35221 gaaatgcgcg cgcggcagtg gcgcgacggc agccagagtg ccgtgcttct ggcgcagctc
    35281 ttcaattgga ccgaactcaa gtgcacgcag cgggcaggat tcgacgcata tgggttgttt
    35341 cccctcggcg acgcgcgaat aacaaccatc gcacttcgtc atgtgccctt tttcagcatt
    35401 gtactgtggt gcgccgtacg gacaagccat gtggcagtag cggcagccga tacaaacatc
    35461 ttcatcaacc accacaaagc catcttcacg cttatgcatt gcgccgctgg ggcagacttt
    35521 tgtacatgcg gggtcatcgc aatggttaca ggagatggag agataataag cgaacacgtt
    35581 ctggtgccag atgccattat cttcctgcca gtcgccgcca gcatattcat aaatacggcg
    35641 gaaactgact tccgggccaa gatctttaaa atctttgcac gccagttcgc aggttttaca
    35701 gccagtacag cggctggaat cgataaaaaa tccatattgt gtggtcatgg gctactcctt
    35761 aaaccttttc gatctggaca agattgctgt gcgacgggtt tccctttgcc agcggtgaag
    35821 ggcggtgaga ggtcagaata ttgatactgc cgccgtgatc gacccggtca ccaaacatat
    35881 ccgctttaag ccacgcacct tgcccgatgg cggtaacgcc aggcagaata cgcggagtca
    35941 cttttgcggc aatcagcatt tctccattat tgttaaatac ccgcacggta tcgccatgac
    36001 ggataccgcg tgcctgagca tcaatggggt tgatccacac ctcttgtggg caggcctgct
    36061 gtaacacatc aatattgccg tagctggagt gggtacgcgc tttggtttgg cgacttcact
    36121 cagcatccag tagatgggtt tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat
    36181 cacatagccc atattccctg cagattcatg agagataagg tcttcttgct ctgttggcat
    36241 caggtcaggc aacaggatat cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc
    36301 aacaatcatc tcgcacttgc tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc
    36361 atgttgattg atcaatgtgt tactggcgta gcaccataaa aacttgatgg ggacatccag
    36421 tttttctttt ccacgaacac catcacgggt cgcggtcatt ttcgtaccat ggtcgatggc
    36481 atctgtccat gtaaaaacgg aaatctgcgt tttaacagga ttctcgagca tcgggaacca
    36541 ttctaccccc agatcccagc taccttcgcg tacacctgag ttgccgccgt ttatgccgac
    36601 gttaccggta agaacggaaa gcatggcaat agcgcgggac gtttgttcgc cgttggagtg
    36661 tcgttgtggc ccccaaccct gacaaatata agcaggtttt gctgaaccga tctctcgtgc
    36721 caactggata attttttctg ccgggatgct ggtgattttt gctgcccatt ccggcgtttt
    36781 agctatgccg tcaggccctt cgcccagaat ataggcttta taatgcgcgt tacgtggtgc
    36841 gttggcgggc agcgtttttt catcgtaacc aacacaatat ttgtcgagaa atggctgatc
    36901 gaccatgttt tcagtaatca gtacccaggc aatcgcacag gccagtgcgc catcggtgcc
    36961 agggcgaatg ggcagccatt catcttcacg cccggcagct gtgtcgttat agcgtggatc
    37021 gatgacgatc atgcgtgcgt ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc
    37081 accgccgctc atccgcgttt ctgccgggtt atttccgaac ataacgacca gtttcgtatt
    37141 ggcgatatca tccgggctgt tgccatcatt ggcaccgaac atataactca ttgcggcact
    37201 gatctgtgcg gtactgtagc tgccatagcg actgagaaaa cccccgcaag agttcatcag
    37261 acggtacggg acgttcgagt tggtgatatt tccgccatct acgcctgttc cgtacaggac
    37321 atgtacagcc tcattgccgt aatctttcag gatccgccga agattatcac tgatggtatc
    37381 cagggcttcg tcccaactta tccgttcaaa tttaccttca ccgcgctcgc cgacgcgctt
    37441 catgggatat ttcaacctgt caggatgatt catccgtcgg cggatagagc gcccgcgtaa
    37501 acatgcccga acctgatgat taccgtaaac gtcgtcacct gtcgtatcag actccaccca
    37561 gtacacggtg tcatctttca catgcaaacg tagcagacag cggctcccgc agttaacggt
    37621 gcaggaactc cagaccgttt tctcttctac cggagcctct gccgcccgga ccatttggga
    37681 aaatggcaga gtgaaagcac tgcttgccag cgcgagactg ccaagtgcgg aggttttcat
    37741 cagacttcta tggctgattt cagccttcat gagcgcctct gtggtatgga ttttcatcat
    37801 tactcactta ttgcttttca aacaaaatgt catgccagaa tttatggttg tagtgggtta
    37861 tattttttcg atctcgacca gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg
    37921 gcgcagagtg gttagcgtat tcacacatcc gccatggtcg attttatcgc cagacatatt
    37981 gggctcgtgc caggctccct ggcccatagc gctaactcca gggagaatgc gtggtgttac
    38041 tttggctggt agccgaactt cgccacgatg gttaaacacc cgcaccatat cgccgttggc
    38101 aatcccacgt ttctgtgcat ctatagggtt gatccacacc tcctgacggc aggctgcctt
    38161 caggacatca atgttgccgt aggtcgagtg agtacgggat ttgtaatgga aaccaaacag
    38221 ttgcagtggg aaggttctac gttcagggga gtcccagcct tcaaaggttg aggcataaac
    38281 tggcagtggg cttatcactt catctttttc cagttcccag gtacgggcaa tttccgccag
    38341 cttgctggaa taaatttcaa tcttaccgga aggcgtttta agtggatttg cctcggggtc
    38401 gtcacgaaat gctttgtagg cgacaaaatg gccattggga tctttacgct tatagatacc
    38461 catttttttc agttcgtcgt aagacggtaa cgccggatct ttggcaagca ttttggcgta
    38521 cagatgttgt aaccattgtt cctgcgtgcg accttctgtg aacttttgat agacgtcagg
    38581 tccaagacgt ttcgcgactt cactcaggat ccagtaaatc ggtttacgtt cgaatttttc
    38641 gctggtgact ggctggagga aaatgagata tcccatgtta ccggcgtagt cgttaggaat
    38701 aatatcttcc tgctcaacgg tcatcaggtc ttccagcaga atgtcggcat attttgccga
    38761 tgaggtcata aagttttcga tgaccacaat catttcgcat ttcgattcgt cctgcagaat
    38821 ttcatgcgtt ttgttgatgt cagaatgctg attaacgagg gtatttcccg cgtagttcca
    38881 gatggactcc attgccagac ggatggatgg acttgaagta gttgactcaa tgctacttaa
    38941 ggtcctgata tgactgacat taatcatgat aacactgtat atccatccag ttctttctct
    39001 gggtctccct atcggtatca gcaagtctac acctcaagcc ctgacacaag gctgacacaa
    39061 atcaatctac tcagcgtagc taagcctcag ttagtccgca gagccaatgg tcgatatact
    39121 atccgcttca gactcaaggg acagaccaca ccgtttcttt cagtatcgac ccgcagcaca
    39181 gacaggagag tagccacaat gcgtcagaga gagcttgcag ccaccgctaa ggccttcatg
    39241 ttggataggc ctgaagtctc actacaggag cttacagagc atctccggtc tatggctgag
    39301 cagttcctga cagacgctag tgacgattac tggaatggac ttgaggtcgc tactctggtc
    39361 gacgagaagt ccaacctgaa ggagctagcc gctacgcaag ccttgagtct ggaccagcag
    39421 aagggcataa ggttagcgct ggaagtcctg accgcagcac agcagagagt cgacactggt
    39481 gatacctctg gtctgattaa gctgattgat gataataacc ttactgatga ctcaactata
    39541 ggcgactcta cgtcaattct gaataatgag cagggggata gaccggcagt tttcacacag
    39601 gagcgccagt catctgtagt tttttcaagc ctagtgtcct cgctgctggc tgagaaggtc
    39661 cagaccctga agacatccag ctacaaggac ctctcgtcct cactcaatac tgtctcccgg
    39721 ttcctgcctg aagacatgga cctgatgtcc cgctctgggt ggctggctgt cagggactct
    39781 atgctggctt ctgaggtgag accatcgaca atcaacaagc tgctgaccaa agcgaagatg
    39841 tgtctcgatt atggcctgat gaatgggcaa cttgagggcc gcaaccctat tgaacggatg
    39901 aagctgacca aggatatcga ctccaagcgc agagcgttca cagacgagga gctggagaga
    39961 cttctggtcc gtgttgagt
//
LOCUS       DAZPFP010000019        37615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPFP010000019 DAZPFP010000000
VERSION     DAZPFP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.019"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHUR4_000947"
     CDS             complement(24..278)
                     /locus_tag="ACHUR4_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1454635.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUR4_000948"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUR4_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1454636.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHUR4_000949"
     CDS             1228..2694
                     /locus_tag="ACHUR4_000949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1454637.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUR4_000950"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUR4_000950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1454638.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUR4_000951"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUR4_000951"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1454639.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUR4_000952"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUR4_000952"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1454640.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHUR4_000953"
     CDS             5709..7265
                     /locus_tag="ACHUR4_000953"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1454641.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHUR4_000954"
     CDS             7347..9161
                     /locus_tag="ACHUR4_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1454642.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHUR4_000955"
     CDS             9162..10256
                     /locus_tag="ACHUR4_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1454643.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHUR4_000956"
     CDS             10256..11281
                     /locus_tag="ACHUR4_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454644.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUR4_000957"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUR4_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1454645.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHUR4_000958"
     CDS             complement(12876..13220)
                     /locus_tag="ACHUR4_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1454646.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUR4_000959"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUR4_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR1454647.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUR4_000960"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUR4_000960"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1454648.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHUR4_000961"
     CDS             15622..17382
                     /locus_tag="ACHUR4_000961"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1454649.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUR4_000962"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUR4_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1454650.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUR4_000963"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUR4_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1454651.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHUR4_000964"
     CDS             19119..19346
                     /locus_tag="ACHUR4_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1454652.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUR4_000965"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUR4_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1454653.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHUR4_000966"
     tRNA            21201..21277
                     /locus_tag="ACHUR4_000966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUR4_000967"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUR4_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR1454654.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHUR4_000968"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHUR4_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1454655.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUR4_000969"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUR4_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1454656.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUR4_000970"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUR4_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1454657.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUR4_000971"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUR4_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1454658.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUR4_000972"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUR4_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1454659.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUR4_000973"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUR4_000973"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1454660.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUR4_000974"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUR4_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1454661.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUR4_000975"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUR4_000975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1454662.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUR4_000976"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUR4_000976"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1454663.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUR4_000977"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUR4_000977"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1454664.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUR4_000978"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUR4_000978"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1454665.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUR4_000979"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUR4_000979"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1454666.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUR4_000980"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUR4_000980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1454667.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUR4_000981"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUR4_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1454668.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUR4_000982"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUR4_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1454669.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUR4_000983"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUR4_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1454670.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUR4_000984"
     CDS             37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUR4_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1454671.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT         9186 a        10163 c         9287 g         8979 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    37501 ttatatagcg attaattcac cagagatatt tctgctggtt tgctccctca tcagaattta
    37561 acactaaaag agcaggtaaa attgtctgaa tgttctttaa gttattcata aagca
//
LOCUS       DAZPFP010000020        36703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPFP010000020 DAZPFP010000000
VERSION     DAZPFP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.020"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            15..371
                     /locus_tag="ACHUR4_000985"
     CDS             15..371
                     /locus_tag="ACHUR4_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1454672.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            375..1502
                     /gene="dapE"
                     /locus_tag="ACHUR4_000986"
     CDS             375..1502
                     /gene="dapE"
                     /locus_tag="ACHUR4_000986"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1454673.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1530..1730
                     /locus_tag="ACHUR4_000987"
     CDS             1530..1730
                     /locus_tag="ACHUR4_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1454674.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1840..2538)
                     /gene="ypfH"
                     /locus_tag="ACHUR4_000988"
     CDS             complement(1840..2538)
                     /gene="ypfH"
                     /locus_tag="ACHUR4_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1454675.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2612..4627)
                     /gene="tmcA"
                     /locus_tag="ACHUR4_000989"
     CDS             complement(2612..4627)
                     /gene="tmcA"
                     /locus_tag="ACHUR4_000989"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1454676.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4642..5505)
                     /locus_tag="ACHUR4_000990"
     CDS             complement(4642..5505)
                     /locus_tag="ACHUR4_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1454677.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5673..6386)
                     /gene="purC"
                     /locus_tag="ACHUR4_000991"
     CDS             complement(5673..6386)
                     /gene="purC"
                     /locus_tag="ACHUR4_000991"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1454678.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6599..7633)
                     /gene="bamC"
                     /locus_tag="ACHUR4_000992"
     CDS             complement(6599..7633)
                     /gene="bamC"
                     /locus_tag="ACHUR4_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1454679.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7650..8528)
                     /gene="dapA"
                     /locus_tag="ACHUR4_000993"
     CDS             complement(7650..8528)
                     /gene="dapA"
                     /locus_tag="ACHUR4_000993"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1454680.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8674..9246
                     /locus_tag="ACHUR4_000994"
     CDS             8674..9246
                     /locus_tag="ACHUR4_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1454681.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9246..9716
                     /gene="bcp"
                     /locus_tag="ACHUR4_000995"
     CDS             9246..9716
                     /gene="bcp"
                     /locus_tag="ACHUR4_000995"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1454682.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            9969..10586
                     /gene="hyfA"
                     /locus_tag="ACHUR4_000996"
     CDS             9969..10586
                     /gene="hyfA"
                     /locus_tag="ACHUR4_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1454683.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10586..12603
                     /gene="hyfB"
                     /locus_tag="ACHUR4_000997"
                     /pseudo
     CDS             10586..12603
                     /gene="hyfB"
                     /locus_tag="ACHUR4_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12614..13561
                     /gene="hyfC"
                     /locus_tag="ACHUR4_000998"
     CDS             12614..13561
                     /gene="hyfC"
                     /locus_tag="ACHUR4_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1454684.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13578..15017
                     /gene="hyfD"
                     /locus_tag="ACHUR4_000999"
     CDS             13578..15017
                     /gene="hyfD"
                     /locus_tag="ACHUR4_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR1454685.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15029..15679
                     /gene="hyfE"
                     /locus_tag="ACHUR4_001000"
     CDS             15029..15679
                     /gene="hyfE"
                     /locus_tag="ACHUR4_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1454686.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15684..17276
                     /gene="hyfF"
                     /locus_tag="ACHUR4_001001"
     CDS             15684..17276
                     /gene="hyfF"
                     /locus_tag="ACHUR4_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1454687.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17266..18980
                     /gene="hyfG"
                     /locus_tag="ACHUR4_001002"
                     /pseudo
     CDS             17266..18980
                     /gene="hyfG"
                     /locus_tag="ACHUR4_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            18990..19535
                     /gene="hyfH"
                     /locus_tag="ACHUR4_001003"
     CDS             18990..19535
                     /gene="hyfH"
                     /locus_tag="ACHUR4_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1454688.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19532..20290
                     /gene="hyfI"
                     /locus_tag="ACHUR4_001004"
     CDS             19532..20290
                     /gene="hyfI"
                     /locus_tag="ACHUR4_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1454689.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20283..20696
                     /gene="hyfJ"
                     /locus_tag="ACHUR4_001005"
     CDS             20283..20696
                     /gene="hyfJ"
                     /locus_tag="ACHUR4_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1454690.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20726..22738
                     /gene="hyfR"
                     /locus_tag="ACHUR4_001006"
                     /pseudo
     CDS             20726..22738
                     /gene="hyfR"
                     /locus_tag="ACHUR4_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22760..23632
                     /gene="focB"
                     /locus_tag="ACHUR4_001007"
                     /pseudo
     CDS             22760..23632
                     /gene="focB"
                     /locus_tag="ACHUR4_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23726..24787)
                     /locus_tag="ACHUR4_001008"
     CDS             complement(23726..24787)
                     /locus_tag="ACHUR4_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1454691.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25000..26463
                     /gene="bepA"
                     /locus_tag="ACHUR4_001009"
     CDS             25000..26463
                     /gene="bepA"
                     /locus_tag="ACHUR4_001009"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1454692.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26484..26843
                     /gene="arsC"
                     /locus_tag="ACHUR4_001010"
     CDS             26484..26843
                     /gene="arsC"
                     /locus_tag="ACHUR4_001010"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1454693.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26976..27677)
                     /locus_tag="ACHUR4_001011"
     CDS             complement(26976..27677)
                     /locus_tag="ACHUR4_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1454694.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27772..29061)
                     /gene="uraA"
                     /locus_tag="ACHUR4_001012"
     CDS             complement(27772..29061)
                     /gene="uraA"
                     /locus_tag="ACHUR4_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1454695.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29147..29773)
                     /gene="upp"
                     /locus_tag="ACHUR4_001013"
     CDS             complement(29147..29773)
                     /gene="upp"
                     /locus_tag="ACHUR4_001013"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1454696.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30097..31134
                     /gene="purM"
                     /locus_tag="ACHUR4_001014"
     CDS             30097..31134
                     /gene="purM"
                     /locus_tag="ACHUR4_001014"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1454697.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31134..31772
                     /gene="purN"
                     /locus_tag="ACHUR4_001015"
     CDS             31134..31772
                     /gene="purN"
                     /locus_tag="ACHUR4_001015"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1454698.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31944..34010
                     /gene="ppk1"
                     /locus_tag="ACHUR4_001016"
     CDS             31944..34010
                     /gene="ppk1"
                     /locus_tag="ACHUR4_001016"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1454699.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34015..35556
                     /gene="ppx"
                     /locus_tag="ACHUR4_001017"
     CDS             34015..35556
                     /gene="ppx"
                     /locus_tag="ACHUR4_001017"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1454700.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35595..>36703)
                     /gene="pdeF"
                     /locus_tag="ACHUR4_001018"
     CDS             complement(35595..>36703)
                     /gene="pdeF"
                     /locus_tag="ACHUR4_001018"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1454701.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8494 a         9711 c         9552 g         8946 t
ORIGIN      
        1 aaaaaggatt cattatggtt acactttacg gcatcaaaaa ttgtgacacc attaaaaagg
       61 ctcgccgttg gctggaagcc aataacatcg actatcgttt tcatgattac cgcgtcgatg
      121 ggctggacag cgaattattg aacggtttta tcaacgaatt aggctgggaa gcgttactca
      181 acacccgtgg tacaacctgg cgtaaactgg acgaaactac ccgcaataaa atcaccgatg
      241 cggcctctgc ggcggcatta atgactgaaa tgcctgcaat tatcaaacgt ccattgctct
      301 gcgcgcctgg taagcctatg ctgctgggtt tcagtgattc cagttatcag caatttttcc
      361 atgaggtgta gtctatgtcg tgcccggtta ttgagctgac acaacagctt attcgccgcc
      421 cttccctgag tcctgatgac gcaggatgtc aggctttgtt gattgaacgt ttgcaggcga
      481 tcggttttac cgttgaacgc atggattttg ccgatacgca gaatttttgg gcatggcgtg
      541 ggcaaggtga aacgttagcc tttgccgggc ataccgacgt ggtgccgcct ggcgacgccg
      601 atcgttggat caatccgccg tttgaaccca ccattcgtga cggcatgtta ttcgggcgcg
      661 gtgcggcaga tatgaaaggc tcgctggcgg cgatggtggt agcagcagaa cgttttgtcg
      721 cacaacatcc caaccatacg gggcgactgg catttctgat cacttctgat gaagaagcca
      781 gtgcccacaa cggtacggta aaagtcgtcg aggcgttaat ggcacgtaat gagcgtctcg
      841 attactgcct ggtcggcgaa ccgtcgagta tcgaagtggt aggtgatgtg gtgaaaaatg
      901 gtcgtcgcgg atcgttaacc tgcaacctca ccattcatgg cgttcagggg catgttgcct
      961 acccacatct ggctgacaat ccggtacatc gcgcagcacc tttccttaat gaattagtgg
     1021 ctattgagtg ggatcagggc aatgaattct tcccggcgac cagtatgcag attgccaata
     1081 ttcaggcggg aacgggcagt aacaatgtta ttccgggtga actgtttgtg cagtttaact
     1141 tccgcttcag caccgaactg actgatgaga tgatcaaagc gcaggtactt gccctgcttg
     1201 aaaaacatca actgcgctat acggtggatt ggtggctttc cgggcagcca tttttgaccg
     1261 cgcgcggtaa actggtggat gcggtcgtta acgcggttga gcactataat gaaattaaac
     1321 cgcagctact gaccacaggc ggaacgtccg acgggcgctt tattgctcgc atgggggcgc
     1381 aggtggtgga actcgggccg gtcaatgcca ctattcataa aattaatgaa tgtgtgaacg
     1441 ctgccgacct gcagctactt gcccgtatgt atcaacgtat catggaacag ctcgtcgcgt
     1501 gatgagtggt tctgcaagag gaaataagca tggactggct ggctaaatat tggtggattc
     1561 tggtgattgt ctttttggta ggcgtcctgc tgaacgtgat taaagatctc aagcgcgtcg
     1621 accataagaa atttctcgcc aacaagccgg agcttccccc gcatcgtgat ttcaacgata
     1681 agtgggacga tgacgacgac tggccgaaaa aggatcaacc gaagaagtaa gctgagtgct
     1741 caaccgtagg ccggataagg cgttaacgcc gcatccggca atggtgaacg atccctgatg
     1801 cgacgctcac gcgtcttatc aggccaggta atctgtcttt tacatcattt caatcacatc
     1861 atcatcgcca ggcttaccgc cacttagcgc ttggtcgaaa taatgtttcg gaatggtata
     1921 acgcaagtga tccagggcaa attgcatact gcgattgtcg atcgcatggc ctaaatcctc
     1981 cacgatatcc agcgtcacgt caccaccggc actgattaac gcttcctgcg ccgcaactgc
     2041 atgcgccaga tcgataaccg gatcttcacc accatgaatc aagtgaattg tcgtggctgt
     2101 agacgcggtc tccggcaggc tcgcatagcg tccgttaaag gcaatgacgc gcgaagcaag
     2161 gcccggttcg gctttaatgc tctccagcgc cataatcgcg ccttgtgaga aaccaattag
     2221 cgccgtggcg ttagccccta ctccgctctg tttctgccag tagcgtaccg tttcaataaa
     2281 cgtcggcatg atggcatcca cacgcgcctg gcgattatct tccgtaatac cctgcactga
     2341 aaaccactga cgccccgccg gattaccgct cggctccgcg ccaccgacac tcaccaccag
     2401 cgcatcagga aacagcggtg caaaccagct gcctatttcc cccattgcca ccgggttatc
     2461 cccgacaccg tggaaaagca gtaacaactg ttgtgcaggt ttatccgggc tttgaacaac
     2521 aaaatggtca tgtttcatgg cgaactcctt aactgatgtc attaatttta cgccgctgac
     2581 tcacaatgac catgggagaa aattgaagga gtcagtgaaa aaattgccat tgcgttatgc
     2641 gatcgcgcag acgctcagtg cgaacctcat ttagtgcgaa cagcgcctgc gcggcctctt
     2701 cccgctggcg aaccagtaac atcttgcggc ctgaaagttt aagtgtggta cataactgcg
     2761 cgtcactggc gtttttctgt aaacgcccac gcagcgccgg taatgccagt tcactggttt
     2821 gtagcagacg caataagcaa cctaacgatg ttaatagcgg acgatgagcg aaagcaaaac
     2881 cggccagttc aagccagtcg tcgtcagaaa ggatggcatc gtttagtgga tcaacaggaa
     2941 gcatttcgcc attccactgc gcgagagctt gcgcatcgcg acgtaaacgg taatgctcac
     3001 gttcagccag ctgtttaccc gcatcactca tcggtaacag cgccatcgcc gtatagcaac
     3061 cgctgctggc ttcccgatga ttacccatcc gcaccagcac aaaaccgcag cgttgccaga
     3121 aacgccataa ctccccagtg taaccaaaac tcaccgaaag atagtcgagg tcatgcgtat
     3181 attgcaaagc tccagcaata agttgtcgcc ctgtgccttc ccgctgacga gccggatgaa
     3241 ctgctatccg gctaacccgc cttccgcgca atgtcgccgc cagtggattg ctgccgtgcg
     3301 ccgccagcga ctgggccacc agattacccc gcgggcgacg aaaacctgcc cataccgcct
     3361 gactgagttc ttgagataat ccgccctcat ccaccagcca caacgccccg gcaatctcgt
     3421 tttcgccagc cgcctgtaaa aaatgttgcc ctggtgcatc catcatccgg cgtaaatcca
     3481 gcggcgaggt ccggtagtgc gcaccagaca ataactgata aacctttaac ggcgtttctg
     3541 gctcgcttcg ccataacgtt tgttcaaatg cggaaatgac gatattgcct tgtggtgtat
     3601 gggtgaagtt ttcatcgtta aaaaccagtg cctcactaac cattttttcc agcgggcatc
     3661 cctgtgccca gcggatcggc tgttgcagtt caaaacggtg taaatgcgga aagcgagcgc
     3721 aaaatttcag caaaaaacca cgtccggtac cttcgtagcc ctgcaccgta gtggttaaca
     3781 acgttcgagg aaaacgcgat accagttgat gcaacaacgg cgcaggtatg gctgcggctt
     3841 cgtcgaccac cagccagtcg gcttgctcat cgctggctaa caaggcatcc ggtgcaataa
     3901 agcgaaactt ctcgcccgca aattgtgcca gtacatccgt tgccgctttt gcgggcgcgg
     3961 tgacaatcgc actgcccgca atacgagaaa tgagttgccc tgccaacgcc gacttaccgc
     4021 gcccacgcgc agccgttacc gctgccacgc ccggcggcat ggtcagtagc tgctgtaaga
     4081 gttgctgttg ttctggttgt ggtgcgccgg tcgcggggtg ccagtcagta cggggagtaa
     4141 aatgcgccaa cgagaacggc tggttttgcc gccagaggat agcgtcgtta tccgccgtaa
     4201 gtacgcgttt gagatgctgg acaaaatgcg gcgtcgcaat agggtcaggg caatcactcc
     4261 agcgcagcga gtcggcatca ggttggtttt cccactcttc ccatacaggg agtaacaaaa
     4321 ccagccagct tcccgctttc aacgttccgc taagtgctgc aaaggcagcg gcatcaaagc
     4381 cgtggcgggc gtcgaatacc gcatgccgga actcgcgccc aagtaaagtt tgtagcgccg
     4441 agggtgaaca gtggttttca gcatctagct gcggcgaaat ccacagccag tcgccaggta
     4501 aggcatcacg caacttaaga gcatgatcaa aacaccaacc ctcttccccg ctcaacacca
     4561 gcaagcggca gatcccttca cgtttcattt gcgctgttaa tgtgtaaagc gcagtcagtt
     4621 cagccatccc tgccccggaa attaaatgct tttaccaaaa gtattgcatt gtgccggatc
     4681 gccgctgtcg aaaccacgtt taaaccagct gtagcgttgc tgagaagtgc catgtgtgaa
     4741 actgtctggt actactcgcc cctgactttg ctgttgtaaa cggtcatcgc cgatggcctg
     4801 cgccgcattc agcgcctctt ccagatcgcc agtttccaga acgccttgct gctgcatact
     4861 atgcccccag acaccggcaa aacagtcggc ctggagttcc atacgcacag ataagcggtt
     4921 tacttccgcc tgcgtcgcgt tttgttgcag ttgacgaact ttcggctcga tgcctaacag
     4981 tttctgcaca tgatgaccga cttcatgggc gataacgtac ccctgggcaa aatcgccatc
     5041 cgcgccaagt ttgtctttca tgtcatcata gaaggagaga tcgatataaa ctgtgccatc
     5101 cgccgggcaa tagaacggcc ccataatgga ctggcccgcc ccgcagccgg tgcgcgtcat
     5161 tccacggtac atgaccagct tcggttgctg ataggtctta cccatctttt cgaactgttg
     5221 tccccaggtg tcttccgtgg ttgcctgaat caccgaggtg aattttgcgg cttcgtcttc
     5281 attagggcta attgaccgcg ttgattgttg ttgagaaacc ggctgcccgg tcatcaaccc
     5341 ggttaaatca acgccatagt agcctgcaac cagcacgact atcagtaaaa tcagcccgcc
     5401 tttaccgctc ggcagacgga aaccgggacc gcccattgac ggaccgccag agctgttgcg
     5461 cctgtcttca acattgtcac tttcacgtcg cccttgccaa cgcatagata cctcaacaat
     5521 atattcatta tagaaatgat cgtaggtggt tcagaggaag attaccacag gaaagcggga
     5581 tgaaatggcg gaggaatgag cgttcaatgc atatattcca agagcggttg tcttgccgtg
     5641 ccagctgcac ggcaagatga tgaacagaaa aatcagtcca gctgtacacc caggcggcgg
     5701 gcgacggctt cataggcttc gatcaggcca ccgaggctct ggcggaaacg gtctttgtcc
     5761 attttctcca gtgtttcttt gtcccacagg cggctgccgt ccggggagaa ctcatcgccc
     5821 agtaccactt cacctttgta cagaccaaac tccagcttga agtcgaccag aatcagacca
     5881 gcatcatcga acagtttttt cagcacgtcg ttcgctttgt aggtcagctc tttcatacgc
     5941 gccaggttct ctttgctcac ccagccaaag gtttcgcagt aagattcgtt gaccatcgga
     6001 tcgtgcatgg catcgttttt caggaacaga tcgaacagcg gcgggttcag ctcaatacct
     6061 tcttcgattc caagacgttt cactagagag ccagcagcac ggttacgcac gacacactca
     6121 accggcacca tatccagctt tttcaccaga cattcggtat cggagagcag acgctccatt
     6181 tgagtcggga tacccgcttc agccagtttg ctcataatga agtagttgaa cttgttgttc
     6241 accatacctt tgcgatcaaa ctgctcaatg cgcgcgccat cccctgctga cgtatcattg
     6301 cggaattcga gcaccaacag gtccgggttt tccgtgctgt atacggtttt cgctttacca
     6361 cgatacaact cagcttgctt ttgcatcttt atcactcctg ggtgtgaatt aacgttttaa
     6421 aatcttttgc tgtctggtgt gccggatgtt ttgtcggatg cggcgtgaac gccttatccg
     6481 aactacgtcc gacaaacaaa tttcgtgcga attaccgcta aaatcgcgtt ttcctgccga
     6541 cgcacacgtt tgcgtatcat atcagaaaaa agggccggat gattccagcc ctgtattttt
     6601 acttgctaaa cgcagcctgg aagacagcta ccagcgcgtc gttctgactc tgagtcagag
     6661 tatgaccttt cggatcgatg aactgtaggc tgctgcggtt atctaaatcg ccaacctgca
     6721 gtttatagtc accggatgcc aggcctggat cgctcgcgcc cagttcctgc cagtcgctgt
     6781 cagacagcgg cttataagtt acggccatgt tgccctgcga acgggtgctg tcggtcactt
     6841 tcatgcccac tttttccagc gccgctggca gacgttgcca aaccacattg aacggcccgc
     6901 gtacgaccag cattggtaaa ccggtgtcat cagctgcact ttgtacgtcc atagtggtgg
     6961 aggcacgatt ttgcgcagcg ttcgcggcgt cagtggcaga tttatccaga ccggcggaaa
     7021 taacgttcat catctccgtg ctgtaacgct gcatggaagc cgcgtctgca accggtttgc
     7081 ccgcctgttc caggttcagc agtttaaccg taaccgcctg ctgataaccc tgcggcttaa
     7141 cagagatttg ataacgacca cgatactgct cgtcttcgtc cagacggttc cattgtaccc
     7201 aatcggtggt cagtgtctga ccagcatcat cacgttgggt gatggtgtag tttttcgcct
     7261 gcagcacgct aaccacctgc ggccacagag tattgccacg accattttcc accagcaatg
     7321 aagcggtatc gcccgtgaac tgggtacgcg cgccagaaac cagtgccaac ggctgggctg
     7381 gtggacgaat gtccagcgcc ttaccgacag caccactacc gttggtcacc gggattgcat
     7441 aatcaccgga ggtcaccggc aaaatcattc cagccggggc atgaagctcc gcaagcggtg
     7501 ccgcttccag gtaggcttca tcaccactga cctgacgctt atagcgtgag tcagaactac
     7561 aggcagcgag taataaaaca agcgaaacac ccgcaacctt tgccaggcgc gacttttgaa
     7621 cagagtaagc catcaaatct ccctaaactt tacagcaaac cggcatgctt aagcgccgct
     7681 ctgaccgtct cacgaccact gtcggtgatt ggtgtcattg gcaggcgcag cgtatcggtc
     7741 gccacaagac ccagttcctt acatgcccat ttcaccggga ttggattggg ttcgacaaat
     7801 agtttgttgt gtaatggcat cagacgctga ttaataacgc gtgcctcggc aaaatgccct
     7861 tctgctgcca gtttgcacat ctgggccata tcacgcgctg cgacgttagc cgtaacggaa
     7921 ataactccat gaccgcccaa ttgcatgaag tccagcgcgc tcgcatcatc gccgctcagc
     7981 agaacaaaat catctgaaac cagctctttg atctggttta cacgcgttaa gttccctgtt
     8041 gcctctttga ttccgataat attttttact ttcgccagac ggcccaccgt ttccgggagc
     8101 agatcgcagc cagtacggga cggcacatta tacagaattt gcggcaggtc agtatgctca
     8161 gcgatggctt tgaaatgctg atacaaacct tcttgcgacg gacgattgta gtaaggggtt
     8221 accgtcaggc agccgacgat accactgtca ttgaagcgct gcgtcaggct aatggcttcc
     8281 gcagtagcgt tagcgccggt cccggcaatt accggaatgc gcccatcagc cagatccagc
     8341 gtcatcatca ccacatcagc atgttcgtca tgatttaagg tagcggactc gccagtggtg
     8401 ccaacagaaa cgatcgccga agtaccgctg gcgacatgat aatcaatcag ttttttcaag
     8461 ctagcccgac agacattacc tttttcatcc atcggagtaa caatcgcgac aatacttccc
     8521 gtgaacatgg gccatcctct gtgcaaacaa gtgtctcaat ggtacgtttg gtatggcatt
     8581 aaaagcaagc agacagaacc gttctgattg ttgtatgcat gattttttta tgctttcctt
     8641 aagaacaact caccccttaa aggaataacc agtttgacac tgtcatcgca acattatctg
     8701 gtgatcactg cgttgggtgc cgatcgccct ggaattgtga acaccatcac ccgtcatgtc
     8761 agtagttgcg gctgtaatat tgaagacagt cgcctggcga tgctgggaga agagttcacg
     8821 tttatcatgc tgctttccgg ttcatggaat gccatcactc tgattgaatc aacgttaccg
     8881 ttgaaaggtg ccgaactgga tcttttaatc gtgatgaagc gcacgacggc gcgtccgcgt
     8941 ccgccaatgc cagcatctgt ctgggttcag gtcgatgttg cagactcccc gcatttaatt
     9001 gaacgcttca cagcactttt cgacgcgcat catatgaaca ttgcggagct ggtgtcgcgc
     9061 acacaacctg ctgaaaatga acgggctgcg cagttgcata ttcagataac cgcccacagc
     9121 cccgcatctg cggacgcagc aaatattgag caagcgttca aagccctatg tacagaactc
     9181 aatgcacaag gcagtattaa cgtcgtcaat tattcccaac atgatgaaca ggatggagtt
     9241 aagtaatgaa tccactgaaa gccggtgata tcgcaccgaa atttagcttg ccggatcaag
     9301 acggagaaca agttaatttg accgacttcc agggacagcg tgttctggtt tatttctacc
     9361 cgaaagccat gacccccggc tgtaccgtac aggcctgcgg cttacgcgat aacatggatg
     9421 agttgaaaaa agcgggcgtt gatgtgctgg gtatcagcac cgataaaccc gaaaaactct
     9481 cccgttttgc ggaaaaagag ctgcttaact ttacgctcct gtctgatgag gaccaccagg
     9541 tgtgcgaaca attcggcgtc tggggtgaaa agtccttcat gggcaaaacc tacgatggta
     9601 ttcatcgcat cagcttcctg attgacgctg atggcaaaat cgaacatgtc tttgacgatt
     9661 tcaaaaccag caatcaccac gacgttgtgc tgaactggct gaaagaacac gcctgattac
     9721 tttgctccat tccgtgctgg ctgcgcttgc ggccagcata cctcacttct cgtgatcaag
     9781 atcacattct cgctttcccc tgcgacacgg gtgtcgaatc cattttttgc tgaacgttaa
     9841 tgaccatcat ttttgtaccg ttcagaatcc agttaataca taacttattg aatagattga
     9901 gttaatcaga atggcatcct ttatacaata tgaaatgcaa tgtttcatat cattttcaag
     9961 gagccgacat gaaccgcttt gtggtggccg aaccactgtg gtgtacagga tgtaatacct
    10021 gtctcgctgc ctgttcggac gtgcataaaa cgcaaggttt acagcaacac ccgcgcctgg
    10081 ccctggcgaa gacgtcaaca atcactgccc ctgtcgtgtg tcatcactgt gaggaagccc
    10141 cttgcctgca ggtctgcccg gtcaatgcca tctctcagag ggatgatgcg atccaactca
    10201 acgaaagcct ctgtattggc tgcaagcttt gcgccgtggt ctgcccattt ggcgcaatca
    10261 gcgcttcagg aagccgtccg gtgaatgccc atgcgcaata tgtttttcag gctgaaggct
    10321 cactcaaaga cggcgaagaa aacgtgccgc cccaacatgc gttgctgcgc tgggaaccgg
    10381 gtgtccagac cgtcgcggtg aaatgcgacc tgtgtgattt cttgccagaa ggtccggcct
    10441 gcgttcgcgc ttgcccgaat caggcgttac ggctgatcac cgacgatagc ctgcaacgtc
    10501 agatgaaaga aaaacagcgc cttgccgcaa gctggtttgc caatggcggg gagtctcccc
    10561 tttccctcac tcaggagcaa cactaatgga tgccctgcaa ttattaacct ggtcgctgat
    10621 cctctatctg tttgccagtc tggcttcgct gtttttactc ggtctggaca gactggctat
    10681 taagctttcc ggcatcacat cgctggtggg cggcgtgatt ggcatcatca gcggaattac
    10741 gcaattacat gctggtgtaa ctttagtcgc ccattttgcc accccttttg actttgccga
    10801 tttaaccctg cgaatggata gcctctcggc atttatggtg ctagttatct ccttgctggt
    10861 ggtggtttgt tcgctctatt cattgactta tatgcgcgaa tacgagggca aaggcgcggc
    10921 ggcgatgggc ttctttatga atcttttcat cgcatcgatg gttgccctgc tggtgatgga
    10981 caacgctttt tggttcatcg tgctgtttga aatgatgtcg ctgtcttcct ggtttctggt
    11041 cattgccagg caggataaaa cgtcgatcaa cgctggcatg ctctactttt ttatcgccca
    11101 cgccggatcg gtgctgatta tgatcgcctt cttgctgatg gggcgcgaaa gcggcagcct
    11161 cgattttgcc agtttcgcac gctttcactt tctccggggc tggcgtcggc ggtgtttctg
    11221 ctggcctttt tcggttttgg cgcgaaagcc gggatgatgc cgttgcacag ctggttgccg
    11281 cgcgctcacc ctgccgcacc atcgcacgct tcggcgttga tgtctggcgt aatggtcaaa
    11341 atcggtattt tcggcatcct gaaagtagcg atggatctgc tggcgcaaac gggtttgcct
    11401 ctgtggtggg gcattctggt gatggcgatc ggcgcaatct ccgcgctcct gggcgtgcta
    11461 tatgcgctgg cggaacagga tatcaaacgg ctgctggcct ggagtaccgt cgaaaacgtc
    11521 ggcattattt tgctggcagt cggtgtggcg atggacggtc tgtcactgca cgacccgctg
    11581 ctcaccgtgg ttggactgct cggcgcactg tttcatctgc tcaaccatgc gctgttcaaa
    11641 gggctgctat ttctcggcgc ggacgcgatt atttcgcgtt tgcataccca cgacatggaa
    11701 aaaatggggg cactggcgaa acggatgccg tggacagccg cagcatgcct gattggttgc
    11761 ctcgcgatat cagccattcc tccgctgaat ggttttatca gcgaatggta cacctggcag
    11821 tcgctgttct cactaagtcg tgtggaagcc gtagcgctac aacttgcggg tcctattgct
    11881 atggtaatgc tggcagtcac tggtgggctg gcagtaatgt gcttcgtcaa aatgtacggt
    11941 attactttct gtggtgcgcc gcgcagtaca cacgctgaag aggcacagga agtgccaaat
    12001 acgatgatcg tcgccatgct actgctcgcg gcactctgcg tattaattgc gcttagtgcc
    12061 agttggctgg caccgaagat aatgcatatt gcccatgcgt ttaccaatac ccctcccgtc
    12121 actgtcgcca gcggaatagc acttgtaccc ggcacgtttc atacacgggt cactccttca
    12181 ttactgttgc tgttactact ggcgatgcct ttgctgccag gcctttactg gctgtggtgt
    12241 cgttcgcgcc gcgcagcgtt tcgtcgcaca ggagatgcct gggcatgcgg ctacggctgg
    12301 gaaaacgcga tggctccgtc aggcaatggc gtgatgcagc cgctgcgtgt ggtcttctct
    12361 gcgttatttc gtctacgaca acagctcgac cctacgctga ggctaaataa aggtcttgcg
    12421 cacgtcaccg ccagggctca gagcacagaa cccttctggg atgagcgggt gatccgcccc
    12481 atcgtgagcg ccacccaacg gctggtcaaa gaaatacagc atctgcaaag cggcgacttt
    12541 cgtctctatt gcctgtatgt ggtcgccgca ctggttgtgc tgctaatcgc tattgccgtc
    12601 taaggaaatc accatgagac aaactctttg cgacggatat ctggtcattt ttgcgttagc
    12661 acaggccgtg attctgctga tgctaacccc actttttacg ggtatttccc ggcagatacg
    12721 cgcgcgtatg cactcccgcc gcgggccggg gatctggcag gattatcgcg atatccacaa
    12781 actgtttaaa cgccaggaag ttgcgccgac atcttcaggt ctgatgttcc gcctgatgcc
    12841 gtgggtatta atcagcagca tgctggtgct ggcgatggcc ttaccactat ttattaccgt
    12901 ttcccctttt gcgggcggcg gcgatctgat cacccttatc tatcttcttg ccctgtttcg
    12961 ttttttcttt gctctttccg ggctggatac cggaagtccg tttgcgggag tcggtgccag
    13021 tcgcgagttg acgctcggca ttctggtcga accaatgctt attctctcac tgctggtatt
    13081 ggcgctgata gcaggttcca cgcatatcga gatgatcagc aatacgctgg ctatgggctg
    13141 gaactcgccg ctaaccaccg tactggcgtt actggcctgt ggttttgcct gcttcattga
    13201 gatgggaaaa attccctttg atgttgctga agcagaacag gaattacagg aaggcccgct
    13261 gaccgaatat tccggtgccg ggctggcgct agcgaaatgg gggctggggc tgaaacaggt
    13321 cgtgatggca gcactgtttg tggccctgtt tctgcccttt gggcgcgcgc aagaactttc
    13381 tctcgcctgc ctgctgactt cacttgtcgt tacgctgctc aaggttttgc tgatttttgt
    13441 actggcctca atcgcagaaa acacgctggc acgcgggcgt tttttactca ttcaccatgt
    13501 gacctggctt ggcttcagcc ttgctgcgct tgcatgggtc ttttggttaa ccggtctgta
    13561 aggagcactg acggaatatg gaaaatcttg ctctgacgac gttaatgctg ccttttatcg
    13621 gcgcactggt cgtttcgttt tcgccacaac gtcgggccgc cgaatggggg gttttgttcg
    13681 ccgcgctgac cacgctgtgc atgttgtcgc tgatctccgc gttttatcag gccgataaag
    13741 ttgccgtcac gttgacgttg gtcaacgtgg gggatgtggc attatttggc ctggtcattg
    13801 atcgcgtgag tacgctgatt ctgtttgtgg tggtgtttct cggtttgctg gtcacgatct
    13861 actccacggg ttatctgacg gataaaaatc gcgaacaccc gcataacggc acgaatcgtt
    13921 attacgcatt tttgctggtg tttatcggcg cgatggcggg actggtactc tcctcgacgc
    13981 tgctcggtca gttgttgttt tttgaaatta cgggcggctg ctcctgggcg ttgatcagtt
    14041 attaccagag cgataaagcg cagcgttcag cactaaaagc gttgcttatc acccatatcg
    14101 gttcgttggg gttgtatctt gccgccgcca cgctgttttt gcagaccgga acgtttgtgc
    14161 ttagcgcgat gagcgagtta catggcgacg cacgttatct ggtttatggc ggcatcctgt
    14221 ttgccgcgtg ggggaaatcg gcccagctac cgatgcaagc gtggctaccg gacgcaatgg
    14281 aagcgccaac accgatcagc gcctatctcc acgccgcatc gatggtgaaa gtgggcgttt
    14341 acatttttgc ccgcgccatt atcgacggcg gcaatatccc gcatgtgatt ggcggcgttg
    14401 gcatggtcat gacactggtc accattcttt atggctttct gatgtatttg ccacagcagg
    14461 atatgaagcg gttgctggcc tggtcgacca tcactcaact tggctggatg ttcttcggct
    14521 tgtcgctctc catcttcggc tcgcggctgg cgctggaggg tagcatcgcc tacatcgtca
    14581 accacgcgtt cgctaaaagc ctgtttttcc ttgtagcagg tgcgctgagt tacagctgcg
    14641 gcacgcgctt gttgccgcgt ctgcgtggcg tattgcacac cctgccgttg ccaggcgtgg
    14701 gtttctgcgt ggcagcgctg gcaattaccg gtgtgccgcc gttcaacggc ttcttcagta
    14761 aattcccgct gtttgccgcc ggttttgcgt tgtcagtgga gtactggatc ctgctgcccg
    14821 ccatgattct gctgatgatt gaatcggtcg ccagtttcgc ctggtttatt cgctggtttg
    14881 gtcgcgtcgt gccgggcaaa ccgagcgagg ccgtcgccga tgccgcaccg ctgccagggt
    14941 caatgcgcct ggtgttgatt gtactgattg tgatgtcgct gatttccagc gtaatcgccg
    15001 cgacctggtt gcagtaagga gatgatgaat gaccggttct atgatcgtaa ataatctggc
    15061 gggactgatg atgctgacat cgctgtttgt gattagcgtc aaaagctatc gcctgtcatg
    15121 cggattttac gcctgccagt cgctggtgct ggtgtctatt ttcgccactc tctcgtgcct
    15181 gttcgccaca gagcaactgc tgatctggtc cgccagcgcc tttatcacca aagtgctgct
    15241 ggtaccgtta atcatgactt acgctgcacg aaatattccc cagaacatcc cggaaaaagc
    15301 gttattcggt ccggcaatga tggcactgct cgcggcgtta attgtcctgc tttgcgcatt
    15361 tgtcgttcag cccgtgaagc taccgatggc taccgggctg aaaccggcgc tggcggtagc
    15421 gttaggtcat tttctgcttg gcctgctgtg cattgtcagc caacgcaata tcctgcggca
    15481 aatttttggt tactgcctga tggaaaacgg ctcccatctg gtgctggcgc ttcttgcctg
    15541 gcgagcaccg gaactggtgg aaataggtat cgctaccgac gccatcttcg ccgtcattgt
    15601 gatggtgtta ctggcaagaa aaatatggcg tacccacggc acgctggacg tgaacaactt
    15661 gaccgcgctg aagggataat gagatgagtt attctgtgat gttcgcttta ctcctgctca
    15721 cgccgctgct tttttcgctg ctctgttttg cctgccggaa acgggggctc tctgcgactt
    15781 gtacggtgac tgtattacat agcttaggga tcacactgct gctgattcta gcactctggg
    15841 tggtccaaac tgccgctgat gcaggagaaa tattcgctgc gggactgtgg cttcatattg
    15901 atggtctggg cggtttgttc ctcgccattc ttggtgtgat tggctttctc accggtgttt
    15961 actcgattgg ctacatgcgt catgaagtgg cacacggcga gctttcaccc gttacactgt
    16021 gcgattacta cggtttcttc catctgtttt tgttcaccat gctgctggtt gttaccagca
    16081 ataacctgat tgtgatgtgg gcggcgatcg aagccaccac cttaagctcg gcgtttctgg
    16141 taggcattta cggtcagcgt tcatcgctgg aatcagcatg gaagtacatc attatttgta
    16201 ctgttggtgt cgcttttggt ctgttcggta ccgtgctggt atacgccaac gccgccagcg
    16261 ttatgccgca ggcagaaatg gcgatattct ggagcgaggt tcttaagcaa tcgtccttgc
    16321 ttgacccaac attaatgctg ttggcctttg tgtttgtgct aattggcttt ggtaccaaaa
    16381 ccgggctatt tcccatgcac gcctggctgc cggatgctca cagtgaagcg ccgagtccgg
    16441 tcagcgccct gctctccgcc gtattgctga actgcgcgct gttggtgctg attcgctatt
    16501 acatcattat ttgccaggcc atcggcagcg atttccccaa ccggttgttg ctcatcttcg
    16561 gcatgttgtc ggttgccgtg gcggcatttt tcattctggt acagcgggac attaagcgtc
    16621 tgctggcgta ctccagcgtg gagaacatgg ggctggtcgc ggtggcgcta ggcattggcg
    16681 ggccgctggg aatttttgcc gcgctgctgc acaccttaaa ccacagtctg gcaaaaacgc
    16741 tgctgttctg cggttccggc aatgtactgc tcaagtacgg cacgcgcgat ctcaacgtcg
    16801 tctgtgggat gctcaaaatc atgccattta ccgccgtgct gtttggcggc ggtgcgctgg
    16861 cgctggcgct ggcgctggca gggatgccgc ccttcaacat ttttcttagc gaatttatga
    16921 ccgttaccgc aggactggca cgtaatcacc tgctgattat cgtcctgctg ttattgctgt
    16981 taacgctggt gctggcgggc ctggtacgga tggctgcgcg ggtgttaatg gcgaaaccgc
    17041 cgcaggccgt taaccggggt gatctcggct ggttgaccac ctcgccaatg gtgattctgc
    17101 tggtcatgat gctggcgatg ggaacgcata ttccacaacc tgtcatcagg atcctggcgg
    17161 gcgcttccac tatagtcctc tcagggacgc acgacctgcc tgcacaacgt agcacctggc
    17221 atgatttttt gccttcaggc accgcatctg tttcggagaa acacagtgaa cgttaattca
    17281 tcgtcaaatc gtggcgaagc gattctcgcc gccctgaaaa cgcagttccc cggcgcggtg
    17341 ctggatgaag agcgacaaac gcctgaacag gtcaccatta cggtgaaaat caatctgctg
    17401 cctgacgttg tacattatct ttattatcaa catgatggct ggcttccagt cctgtttggc
    17461 aacgacgagc ggacacttaa cggtcattac gcggtttatt atgccctttc aatggaaggg
    17521 gccgaaaaat gctggatcgt ggtgaaggca ctggtcgatg ccgacagtcg ggagtttccg
    17581 tcagtcacac cgcgcgtccc tgccgcggtc tggggcgagc gagaaattcg cgatatgtac
    17641 gggctgattc cggttggcct gccggatcag cgtcgcctgg tgttgcccga tgactggccg
    17701 gaagatatgc atccgctgcg caaagatgcg atggattatc gactgcgccc tgaaccgacg
    17761 acagattccg aaacgtatgc gtttatcaat gagggcaaca gcgatgcgcg ggtgatccct
    17821 gtcggcccgc tgcatatcac ctcctatgaa ccgggtcact tccgcttgtt tgtggatggc
    17881 gagcaaattg tcgatgctga ttaccgcctg ttttatgtcc atcgcggcat ggagaaactg
    17941 gcagaaacgc ggatgggcta caacgaagtg accttcttat cggaccgcgt gtgtgggatt
    18001 tgcggttttg cccacagtgt ggcctatacc aactcggttg aaaatgcact ggggattgag
    18061 gtgccgcaac gagcacatac tattcgctcg attctgctgg aagtcgagcg gctacacagt
    18121 catttgctca accttggcct ctcctgccat tttgttggtt ttgataccgg ctttatgcaa
    18181 tttttccgcg tgcgggaaaa gtcgatgacg atggcggaat tgctgaccgg gtcgcgtaaa
    18241 acctacggtc tgaatctgat tggtggtgtt cgccgcgata ttctcaaaga gcaacgtctg
    18301 caaacgctga aactggtgcg cgagatgcgc gccgacgtgt cggagctggt agagatgctg
    18361 cttgctacgc cgaatatgga acaacgcact cagggcattg gcattctcga ccgacaaatc
    18421 gcccgtgatt atagccctgt agggccgctg atccgcgcag tggttttgcc cgtgatttgc
    18481 gctttgatca cccctacgcc gactacggca atattccaaa aacactgttt acctttaccg
    18541 gcggcgatgt tttctcccgc gtgatggtcc gtgtcaaaga gacgtttgat tcgctggcaa
    18601 tgctggaatt tgccctcgac aacatgccgg ataccccact gctgaccgaa ggctttagct
    18661 ataaacctca cgcattcgcg ctgggctttg ttgaagcgcc acgcggtgaa gacgtgcact
    18721 ggagcatgct cggtgataac caaaaattgt tccgctggcg ctgtcgtgcc gccacctacg
    18781 ccaactggcc ggtgttgcgt tacatgctgc gcggcaatac cgtttctgac gcaccgctga
    18841 ttatcggtag ccttgatccc tgctactcct gtaccgaccg tgtgacgctg gtagatgtgc
    18901 gcaagcgcca gtcaaaaacc gtgctgtata aagagatcga acgctacggc attgatcgta
    18961 accgttcgcc gctgaagtaa ggacagaaga tgctgaagtt actgaaaact attatgcgcg
    19021 ccggaaccgc gacggtgaaa tatcccttcg cgccactgga ggtcagccca ggctttcgcg
    19081 ggaaaccgga cctgatgccc agccaatgta ttgcctgcgg tgcctgcgcc tgtgcttgtc
    19141 cggcaaatgc gctgactatc cagaccgacg accagcaaaa ttcgcgcacc tggcagctct
    19201 atctggggcg ttgtatttac tgcggacgtt gtgaagaagt gtgcccgacc agagccatcc
    19261 agcttaccaa taactttgaa ctgaccgtca cccataaagc cgatctctat acccacgcga
    19321 cgttccatct acaacgttgc agccgttgcg aacgcccgtt tgccccgcaa aaaaccgtcg
    19381 cactggctgc tgaattgttg gcacagcaac aaaatgctcc acaaaaccgc gaaatgttat
    19441 gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc aacgctgatc aacgacgata
    19501 cagatgtacc gctggtggct aaggagcagc tatgagtcca gtgcttacgc aatatgtcag
    19561 ccagcccatc acgctggacg agcaaacgca aaagatgaag cggcatttgc tacaggatat
    19621 ccgtcgctcg gcttacgttt atcgcatcga ttgcggcggc tgcaacgcct gtgaaatcga
    19681 aatttttgct gccatcaccc ccgtattcga cgcagaacgt tttggcatta aggttgtttc
    19741 atcaccgcgt cacgccgata ttttgttatt tactggcgca gtcacccggg cgatgcgtat
    19801 gcctgcactt cgggcgtatg agtctgcccc cgatcataaa atctgtgttt cctacggcgc
    19861 gtgcggtgtc ggcggcggta ttttccacga tctctacagc gtctggggcg gcagcgacac
    19921 cattgtcccc attgatgttt ggatccccgg ctgcccgcca acaccggccg ccaccattca
    19981 cggtttcgcc gtggcgctcg gtttgctgca acagaagatt cacgcggtgg attatcgcga
    20041 tcccaccggg gtgactatgc aaccgttgtg gccgcagatc ccgccatcac agcgtatcgc
    20101 cattgagcga gaagcgcggc ggctggcggg ctatcgtcag gggcgagaaa tttgcgatcg
    20161 gctcctgcgc catttaagcg acgatcctac aggaaatcgg gttaacacct ggttgcgcga
    20221 tgccgacgat ccacgtctca atagtatcgt tcagcaactc tttcgcgtac tccgggggtt
    20281 acatgactga agagtgcggg gaaattgttt tctggacgct gcgaaaaaag tttgtcgcca
    20341 gtagcgacga gatgccggaa cacagctctc aggtaatgta ttactcgctg gctatcggtc
    20401 atcacgttgg cgtgattgat tgtctgaatg tcgccttccg ctgcccactg acggaatacg
    20461 aagattggct tgcactggtc gaagaggagc aagcccgacg taagatgctg ggggtgatga
    20521 cttttggtga gattgttatt gacgccagcc acaccgccct gttgacccgg gcattcgcgc
    20581 cactggcaga tgacgcgacg tctgtgtggc aagcgcgtag cattcaattc attcatctgt
    20641 tggatgaaat tgtgctggaa ccggctatct atctgatggc cagaaaaatt gcgtgagaag
    20701 gatttctcat taataaggac tgttgatggc tatgtcagac gaagcgatgt ttgccccgcc
    20761 gcaaggaata acaattgaag cggtaaacgg aatgctcgcg gagcggttag cgcagaaaca
    20821 cggcaaggcg tctttattac gcgccttcat cccgctgccg ccgccgttca gcccggtaca
    20881 acttattgaa ctgcatgttc tcaaaagcaa cttctattac cgctaccatg atgatggcag
    20941 cgatgtgacg gcaacaacag agtatcaggg cgagatggtc gattattcgc gttacgccgt
    21001 ccttctcggc agtagtggaa tggcggagct acgctttatt cgtacccacg gcagccgttt
    21061 tactccccag gattgcacac tgtttaactg gctggcacgc attatcaccc cggttctgca
    21121 atcatggctc aatgatgaag cacagcaggt ggcgctgcgt ttgctggaga aagatcgcga
    21181 tcatcatcgg gtactggtgg atatcactaa tgcagtgctg tcacatcttg atctcgacga
    21241 tctgatcgct gacgtcgctc gtgagatcca tcattttttc ggtctggctt cagtcagtat
    21301 ggtactgggc gatcatcgaa agaacgagaa gttcagcctg tggtgcagcg atctttctgc
    21361 ctcacattgt gcgtgtctgc cacacaatat gcctggcgac agtgtattgc tgacacaaac
    21421 gctacaaacc cgacaaccga ccttgacgca ccgtgcagat gatctgtttc tctggcaacg
    21481 cgacccgtta ttactcttac ttgcatctaa cggctgcgaa tctgcgctcc ttataccgct
    21541 tacctttggc aaccatacac cgggtgcatt gttgctggcg catacctctt ccactctctt
    21601 tagtgaggaa aactgccagc tactacaaca catagccgat cgcatcgcta ttgccgttgg
    21661 caatgccgat gcctgccgta gaatgaccga tttgcaggaa agtttgcagc aagaaaacca
    21721 ccagcttagc gagcagctcc tttcgaatct gggcgtcggt gacattatct atcaaagcca
    21781 ggcaatggaa gacctgctcc agcaggtaga tattgtggcg aagagcgaca gtacggtgtt
    21841 gatttgcggt gaaaccggaa ccggcaaaga ggtgatcgcc agagcgatcc atcaacttag
    21901 cccgcgacac gacaagccgc tggtcaaaat caactgcgct gccatccccg ccagtcttct
    21961 ggaaagtgag ttattcggtc atgacaaagg ggcgtttact ggtgcgatta atacccatcg
    22021 tggtcgtttt gaaattgccg atggcggcac gttgtttctc gatgaaattg gcgatctgcc
    22081 gttagaactt cagcctaaac tgctgcgcgt attgcaggaa cgggagattg agcgtctcgg
    22141 cgggagtaga acgatcccgg taaatgtcag agtcattgcc gccaccaacc gtgatttgtg
    22201 gcaaatggtt gaagatcgcc agtttcgcag cgatctcttt tatcgcctga atgtcttccc
    22261 actggaattg cctccgctgc gcgaccgtcc ggaagatatc cctcttttag caaagcattt
    22321 cacgcaaaaa atggcgcgcc atatgaatcg ttcaattgac gccatcccga ccgaggcgct
    22381 acgccagttg atgtcgtggg actggccggg caacgtgcgc gagctggaaa acgtgattga
    22441 gcgagcggta ctgctgactc gcggtaacag tctgaattta catcttaatg tccgacaaag
    22501 ccgtttactg ccgacgctaa atgaagattc agcgcttcgc agttcaatgg cgtagttgct
    22561 gcacccgacg acgccagaga atgacgaaga agaacgtcag cgcattgttc aggtattgcg
    22621 agaaaccaat ggcattgttg ccgggccccg tggcgcggcg acacgattag ggatgaagcg
    22681 caccacgctg ctgtcacgaa tgcagcgtct gggaatctcg gttcgcgagg tgttgtaatc
    22741 tgcttttgca ggagtatgca tgagaaacaa actctctttc gacttgcagt tgagcgccag
    22801 aaaagcggca atcgctgaac ggattgccgc ccataaaatt gcccgcagta aagtgtcggt
    22861 ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatg tccgctggcg tgtttatggc
    22921 gatcggattt actttttacc tttccgttat cgccgatgcc ccgtcttcat aggcattaac
    22981 ccatctggtg ggcggccttt gctttacact tggctttatt ttgctggcgg tttgcggcac
    23041 cagcctgttc acctcgtcgg taatgacggt aatggcaaaa agtcggggcg ttattagttg
    23101 gcgaacttgg ctgattaacg cacttctggt ggcctgcggt aatctggcag gtattgcctg
    23161 tttcagtttg ttaatctggt tttccgggct ggtgatgagt gaaaacgcga tgtggggagt
    23221 cgcgatttta cactgcgccg agggcaaaat gcatcataca tttactgaat ctgtcagcct
    23281 cggcattatg tgcaatctga tggtttgcct ggcgctgtgg atgagttatt gcggacgttc
    23341 gttatgcgac aaaatcgtcg ccatgatttt gtccatcacc ctgtttgtcg ccagtggctt
    23401 tgagcactgt atcgccaatt tgtttgtgat tccgttcgcc attgccattc gccatttcgc
    23461 ccctacctcc ttctggcaac tggcgcacag tagcgcagac aattttccgg cactgacggt
    23521 cagccatttt attaccgcca atctgctccc ggtgatgctg ggtaatatta tcggcggtgc
    23581 agtgctggtg agtatttgtt atcgggctat ttatttacgt caggaatcct gaaaaatcag
    23641 cccggcgaaa cagttcgtcg ggctatttat ttacgtcagg aatcctgaaa aatcagcccg
    23701 gcgaaacagt tcgtcgggct gaagattatt cttgcgcgat ttgcccatcg ggccaggcgt
    23761 ggatcacggc tttgatcagc gtcgccaatg gaatggcgaa gaatacgccc cagaatcccc
    23821 acaaaccacc gaagatcacc accgataaaa taatcaccag cggatgcagg ttaaccgctt
    23881 cggagaacaa caccggtact agcaggttgc cgtccagcgc ctgaataatc agatacaccg
    23941 cgaagcagct ccagaattca gtacctgcgc caaactggaa tagcgccacg ccaaccaccg
    24001 gaatggtcac cacaaatgcg ccaatgtacg gaataagaac cgagaagccg accagcactg
    24061 ccagcagcag cgaatagttc agcccgaaga gcaagaaccc cagccaggtg gcgatcccca
    24121 ccacgatcat ctccagcact ttgccgcgga tatagttggt gatttgttga ttcatctcct
    24181 tccacacctg tcctgccagc ccacggttac gcggcagcac ccggcgaacg gcgttcagca
    24241 tctgctcttt gtctttcagc aggaagaaga ccatcaatgg caccagcacc agatagacgg
    24301 ctatggtcag caaaccgacc agcgaggcga gggaaatttt caccaccgaa tcgcccatgg
    24361 tcagcatccg actgcgcata ttttcggcca ttgcatcaat aatgcccgcg tccattaaca
    24421 ccggatagcg gcgcggcaac gtggcggcaa agtcagaaag cttattgagc atccccggca
    24481 tatcgcggat taagtagatg ccctgttgcc aggcgatagg cagtaccacg aacgccatca
    24541 gtagcaatat accgacaaaa accaccaata caatcgacgt cgcccagcgg cgggagcagc
    24601 caatagattg caggcgcacg gttggccatt ccagcaaata agccagcaca atagccacca
    24661 gcaacggagc aagcaggcca ctaaagaaaa agataatgcc aaatccggca actaaaataa
    24721 ccagcaaggc aatcgcttcc gggtcgctaa aacggcggcg ataccattgc atcaacattt
    24781 cgagcataaa gaccttccct gaacctcaag agcgggattg cgatccgcaa ttgtatcgaa
    24841 atgtcacaaa aaagacttcg ctttttatga cggattcagg aaactgaaaa gtcatttgag
    24901 tgggctaatc ttcgccgtta cactcaaagg cggcgaggtg ggaacgatat ttcacagtat
    24961 cggtcaaatg actatcttca gaaatacagg atagaggtta tgttcaggca gttgaaaaaa
    25021 aacctggttg caaccctcat tgctgctatg accattggtc aggtagcccc ggcgtttgcc
    25081 gacagcgcag acaccttgcc ggatatggga acctccgcag gaagcacgct ttccattggt
    25141 caggaaatgc agatgggcga ctattatgtc cgccagctac gcggcagcgc gccgttaatt
    25201 aatgacccgc tgttaacgca atatattaat tcgctgggga tgcgtctggt ttcgcatgcc
    25261 aattcggtta agacaccgtt tcattttttt ctgatcaaca acgacgaaat taacgccttt
    25321 gctttctttg gcggcaacgt ggtgctgcac tctgccctgt tccgttattc cgataacgaa
    25381 agtcaactgg cttcagttat ggcgcacgaa atctcccacg tcacccaacg tcacctggcg
    25441 cgagcgatgg aagatcaaca acgcagcgcg ccgctgacct gggtcggcgc gttaggttct
    25501 attttactgg cgatggccag tccgcaggcg gggatggcgg cgctgaccgg tacactggcg
    25561 ggaacgcgcc aggggatgat cagtttcacc cagcaaaatg aacaggaagc ggaccgcatt
    25621 ggtattcagg tgctgcaacg ctcgggattc gatccacagg cgatgccgac cttcctggaa
    25681 aaattgctcg atcaggcgcg ttactcctcg cgcccgccgg aaattttatt gactcacccg
    25741 ttgccggaaa gtcgtctggc agatgcccgc aaccgtgcta atcagatgcg cccgatggtg
    25801 gtgcagtcgt cggaagattt ctatctggcg aaagcgcgca cactggggat gtataattcc
    25861 ggacgtaacc agctcaccag tgatttgctg gatgaatggg cgaaaggaaa cgttcgtcag
    25921 caacgagcgg cgcaatatgg tcgtgcttta caggcgatgg aagccaataa atacgacgag
    25981 gcgcgtaaaa cgctgcaacc gttactggcg gcagaacctg gcaacgcatg gtatctcgat
    26041 ctggctaccg atatcgatct tgggcaaaac aaagccaatg aggcaatcaa tcgcctgaaa
    26101 aatgcccgcg atttacgcac caatccggtg ttgcagctca acctggcgaa cgcttatctg
    26161 caaggcggtc aaccacaaga agcggccaat attctgaatc gctacacctt taataataaa
    26221 gatgacagca acggctggga tttgctggca caggcggaag ccgcgctaaa taaccgcgat
    26281 caggagctgg ctgcgcgagc agaaggttat gcgctcgccg gacgactcga tcaggccatt
    26341 tcgctgttga gtagcgccag ttcgcaggtg aaattaggca gcctgcaaca agcgcgttac
    26401 gatgcgcgca tcgaccagtt gcgccagctg cagaaacgct ttaagcctta taccaagatg
    26461 taattcgcat taaaggaaga acaatgacca aacaggtaaa aatctaccat aacccacgct
    26521 gttcaaagag ccgggaaacg ctgaacctgc tgaaagaaaa cggcgtggag ccagaagtgg
    26581 tgctctacct tgagacaccc gccgatgcgg caacgctgcg cgatttgctg aaaatgctgg
    26641 ggatgaatag cgcccgtgaa ttgatgcgcc agaaagagga tctttataaa gaactaaacc
    26701 tggctgacag ttcgcttagc gaagaggcgc tgattcaggc aatggtggat aatccgaagc
    26761 tgatggagcg cccgattatg gtcgcgaatg gcaaagcgcg gattggtcgt ccaccggagc
    26821 aggtactgga aatcgtgggt taagatgtgc tccatcgcta gttgaagcac attgccggat
    26881 gcgacgcttg ccacgtctta tccggcctac gaatactatg gattaacttc gcgtaggtca
    26941 ggcgttcgcg ccgcatccga caataaacac cttatctaca acttcagaat ttctttcaca
    27001 aacggaatgg tcagcttacg ttgcgcggta atcgacgcac gatccaactg atccaacgtc
    27061 ataaatagcg tgcgcatttc tctgtcgagc cgcttcagca agaaacgccc cacatcttcc
    27121 ggcagttcaa aaccacgcaa acgcgcgcgt aactgtagcg cctgcaactt atcttcatca
    27181 gaaagtggct gcaatttgta gatctgcccc cagtcgagac gcgacgcgag atccggtaat
    27241 cccagattca actgccgcgg tggacgatcg ccggtgatca acaaccgtgt tttgcccgat
    27301 tccagaattc gattgtagag atcgaaaatc gccatctccc acaactcatc gcctgcaata
    27361 cactcaatgt tgtcgataca gaccagcgac aaatgctcca taccgtcgag cacttccgga
    27421 acaaaccagg tgcgtttatc cagcgggaca tagcccaccg catcgccacg ctgcgacaat
    27481 tccgcgcaag ccgcgtgcag cagatggctg cgccccgcgc cttcgcgtgc ccagagatag
    27541 atgtaaccgc tatgttcctg acgcagcacg ttttgcagcg cggccagtaa agaggagtta
    27601 tcccccggcc agaaacttgc aaaggtttcg tcgtcaggaa gataaagtgg caaagagagc
    27661 tgtgccggtg tgttcagaga tacctcaacc aggatttcac aaaatcgcga gaagtttacc
    27721 acagaatccc atgatgtttg aaccgggcag caacactgcc cggtcggtac attatttgtc
    27781 tgttatatcc gcgtcttctg cgtccagcac cacttcttca gggcgcagca cactgatcag
    27841 tttgaaaatc aaacttaagc cgataccgac gatggtcgcc agcgccatac cttttaactc
    27901 cgccgcgccg atgtttacct tcgcgccact gacgccgatg atcaaaatca cggaagtcag
    27961 gatcaggttc tgtgctttgt tgtaatccac tttcgattcg atcaaaacac gaataccgga
    28021 agcaccaatg acaccataaa gcagcagcga aacgccgccc atcaccggca atgggatcat
    28081 ctggatagcg gcagccagtt taccgacgca ggaaagcagg atagcgaaaa tcgccgcccc
    28141 gccgataacc caggtactgt aaacacgggt gatcgccatc acgccaatgt tttctccgta
    28201 agtggtattt ggcgtagagc caaagaagcc ggagatcacg gtcgacaggc cgttagcaaa
    28261 catcgaacgg tgcagacctg gatcgcgcag cagatctttt ttgacgatat tagccgttac
    28321 taccaggtgc cctacgtgtt cggcaataac cactaacgcc gctggcagaa tagtcagaat
    28381 ggcaaaccac tcgaagcgcg gcgtatagag ggttggcagt gcaaaccagt gagcattaat
    28441 aatcggcgtg gtatcgacaa ttcccattgc gaaagagagc gcgtacccca ccagcacgcc
    28501 aattaaaatc gggataattg ccaggaaacc acgaaacagc acggaaccta aaaccgtgac
    28561 cgccagggtg gtaatagaga tgatgatggt tttggagttt ggcgtttgcc cttcagccgg
    28621 gagtaaaccc gccataccgg cagctacgcc cgccagctcc agaccgatga cggcaacgat
    28681 tgcgcccatt gccgcaggtg gaaacagcac gtccaaccag ccggtccccg ctttcttcac
    28741 gataaaagaa accaggcaga acagcacgcc gcacataata aagccgccca gcgcgacttc
    28801 ataccctaac ggcaacagta acaataccgg tgaaataaag gcaaagctgg aaccaagata
    28861 agccggaatt ttccctttac agatgaagag atacagcagc gttccaatac cgttaaataa
    28921 cagtacagtc gccgggttaa tatgaaataa gacgggcacc aggacggttg caccaaacat
    28981 ggcgaacaaa tgttgcaaac taagcgggat tgtctgtaaa agtggcggtc tttcactcac
    29041 cccgatagca cggcgcgtca tagtattatc ctctgtatta tgtgttatag gcgctttact
    29101 caaaaaaaag ccgactctta aagtcggctt taattatttt tattctttat ttcgtaccaa
    29161 agattttgtc accggcatcg ccgaggcccg gaataatgta tccgtgctcg ttcagtccct
    29221 ggtcaatcga tgcagtatac agttcgacgt ccgggtgcgc tttttccagc gcagcgatac
    29281 cttctggcgc agcgaccagc accagaactt tgatgctgct gcagcccgct tttttcagca
    29341 ggtcgatggt cgcgataacg gaaccaccgg ttgccagcat tgggtcaacg atcagcgcca
    29401 tacgctcatc gatgttagaa accagtttct ggaagtacgg taccggctcc agcgtttctt
    29461 cattacggta cataccgaca acgctgatgc gcgcgctcgg aacgttttcc agcacaccgt
    29521 ccatcatacc aagacccgca cgcagaattg gcacaacggt aattttctta cctttgatct
    29581 ggtcgatttc taccgggccg ttccagcctt cgatagttac tttttccgtt tcgaggtcgg
    29641 cggtcgcttc gtaagtcagc aggctaccca cttcggaagc gagttcgcga aagcgcttgg
    29701 tgctgatatc ttgctcacgc atcagtccca gcttgtgttt gacgagtggg tgtttgactt
    29761 ccacgatctt catactcttt ctcctttgag gggcagccac aaaaaaatcg acggattata
    29821 cctcctttct tcagggcggc aatattattt tcgttgactt tagtcaaaat gataacagtt
    29881 tgagataaag ttattttata ttcaaatggt tatgaaagaa gattattcca tccgaaaact
    29941 aacctttgcc ctggcacaag tcttctttcg ccgcgcgccc ggggaaaaga cgtgcaaaaa
    30001 ggttgtgtaa aacagtctcg caaacgtttg ctttccctgt tagaattgcg ccgaatttta
    30061 tttttctacc gcaagtaacg cgtggggacc caagcagtga ccgataaaac ctctcttagc
    30121 tacaaagatg ccggtgttga tattgacgcg ggtaatgctc tggttggaag aatcaaaggc
    30181 gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc tgggcggctt cggtgcgctg
    30241 tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt ccggcactga cggcgtaggt
    30301 accaagctgc gtctggcaat ggacttaaaa cgtcacgaca ccattggtat tgatctggtc
    30361 gccatgtgcg ttaatgacct ggtggtgcaa ggtgcggaac cgctgttttt cctcgactat
    30421 tacgcaaccg gaaaactgga tgttgatacc gcttcagcgg tgatcagcgg catcgcggaa
    30481 ggttgtctgc aatcgggctg ttctctggtg ggtggcgaaa cggcagaaat gccggggatg
    30541 tatcacggtg aagattacga tgtcgcgggt ttctgcgtgg gcgtggtaga aaaatcagaa
    30601 atcatcgacg gctctaaagt cagcgacggc gatgtgctga ttgcactcgg ttccagcggt
    30661 ccgcactcga acggctattc gctggtgcgc aaaattcttg aagtcagcgg ttgtgatccg
    30721 caaaccaccg aacttgatgg taagccatta gccgatcatc tgctggcacc gacccgcatt
    30781 tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg tgcatgccat tgcgcacctg
    30841 accggcggcg gcttctggga aaacattccg cgcgtattgc cagataatac ccaggcagtg
    30901 attgatgaat cttcctggca gtggccggaa gtgttcaact ggctgcaaac ggcaggtaac
    30961 gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg tcgggatgat tatcgccctg
    31021 cctgctccgg aagtggacaa agccctcgcc ctgctaaatg ccaacggtga aaacgcgtgg
    31081 aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg tggttatcga ataatgaata
    31141 ttgtggtgct tatttccggc aacggaagta atttacaggc aattattgac gcctgtaaaa
    31201 ccaacaaaat taaaggcacc gtacgggcag ttttcagcaa taaggccgac gcgttcggcc
    31261 ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct catcgccagc gcgtttgaca
    31321 gtcgtgaagc ctatgaccgg gagttgattc atgaaatcga catgtacgca cccgatgtgg
    31381 tcgtgctggc tggttttatg cgcattctca gcccggcgtt tgtctcccac tatgccgggc
    31441 gtttgctgaa cattcaccct tctctgctgc cgaaatatcc cggattacac acccatcgtc
    31501 aggcgctgga aaatggcgat gaagagcacg gtacatcggt gcatttcgtc accgatgaac
    31561 tggacggtgg cccggttatt ttacaggcga aagtcccggt atttgctggt gatacggaag
    31621 atgacatcac cgcccgcgtg caaacccagg aacacgctat ttatccactg gtgattagct
    31681 ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc gtggctggat ggtcaacgtc
    31741 tgccgccgca gggctacgct gccgacgagt aatgcccccg tagttaaagc gccagctctg
    31801 ccgctggcgt ttttcaattc acctgtaaat cgcaagctcc agcagttttt ttcccccttt
    31861 tctggcatag ttggacatct gccaatattg ctcgccataa tatccaggca gtgtcccgtg
    31921 aataaaacgg agtaaaagtg gtaatgggtc aggaaaagct atacatcgaa aaagagctca
    31981 gttggttatc gttcaatgaa cgcgtgcttc aggaagcggc ggacaaatct aacccgctga
    32041 ttgaaaggat gcgtttcctg gggatctatt ccaataacct tgatgagttc tataaagtcc
    32101 gcttcgctga actgaagcga cgcatcatta ttagcgaaga acaaggctcc aacgctcatt
    32161 cccgccattt actgggcaaa attcagtccc gggtgctgaa agccgatcag gaattcgacg
    32221 gcctctacaa cgagctattg ctggagatgg cgcgcaacca gatcttcctg attaatgaac
    32281 gccagctctc cgtcaatcaa caaaactggc tgcgtcatta ttttaagcag tatctgcgtc
    32341 agcacattac gccgatttta atcaatcctg acactgactt agtgcagttc ctgaaagatg
    32401 attacaccta tctggcggtg gaaattatcc gtagcgatag catccgttac gcgctgctgg
    32461 agatcccatc agataaagtg ccgcgctttg tgaatttacc gccagaagcg ccgcgtcgac
    32521 gcaagccgat gattcttctg gataacattc tgcgttactg ccttgatgat attttcaaag
    32581 gcttctttga ttatgacgcg ctgaatgcct attcaatgaa gatgacccgc gatgccgaat
    32641 acgatttagt gcatgagatg gaagccagcc tgatggagtt gatgtcttcc agtctcaagc
    32701 agcgtttaac tgctgagccg gtgcgttttg tttatcagcg cgatatgccc aatgcgctgg
    32761 ttgaagtgtt acgcgaaaaa ctgactattt cccgctacga ctccatcgtc cccggcggtc
    32821 gttatcataa ttttaaagac tttattaatt tccccaatgt cggcaaagcc aatctggtga
    32881 acaaaccact gccgcgttta cgccatattt ggtttgataa agcccagttc cgcaatggtt
    32941 ttgatgccat tcgcgaacgc gatgtgttgc tctattatcc ttatcacacc tttgagcatg
    33001 tgctggaact gctgcgtcag gcttcgttcg acccgagcgt actggcgatt aaaattaaca
    33061 tttaccgcgt ggcgaaagat tcacgcatca tcgactcaat gatccacgcc gcacataacg
    33121 gtaagaaagt cactgtggtg gttgagttac aggcgcgttt cgacgaagaa gccaacattc
    33181 actgggcgaa gcgcctgacc gaagcaggcg tgcacgttat cttctctgcg ccggggctga
    33241 aaattcacgc caaactgttc ctgatttcac gtaaagaaaa cggtgaagtg gtccgttacg
    33301 cacacatcgg gaccgggaac tttaacgaaa aaaccgcgcg tctttatact gactattcgt
    33361 tgctgaccgc agatgcgcgt atcaccaacg aagtacggcg ggtatttaac tttattgaaa
    33421 acccataccg cccggtgaca tttgattatt taatggtgtc accgcaaaac tctcgccgtc
    33481 tgttatatga aatggtagac cgcgaaatcg ccaacgcgca gcaagggctg cccagtggta
    33541 tcaccctgaa gctaaataac cttgtcgata aaggcctggt tgatcgtctg tatgcggcct
    33601 ccagctccgg cgtaccggtt aatctgctgg ttcgcggaat gtgttcgctg atccccaatc
    33661 tggaaggcat tagcgacaac attcgtgcca tcagtattgt tgaccgttac cttgaacatg
    33721 accgggttta tatttttgaa aatggcggcg ataaaaaggt ctacctttct tccgccgact
    33781 ggatgacgcg caatattgat tatcgtattg aagtggcgac accgctgctc gatccgcgcc
    33841 tgaagcagcg ggtgctggac atcatcgaca tattgttcag cgatacggtc aaagcacgtt
    33901 atatcgataa agaactcagt aatcgctacg ttccccgcgg caatcgccgc aaagtacggg
    33961 cgcagttggc gatttacgac tacatcaaat cactcgaaca acctgaataa ccctatgcca
    34021 atacacgata aatcccctcg tccgcaggag tttgctgcgg tcgatcttgg ttcaaacagt
    34081 tttcacatgg tcatagcccg tgtggtagat ggtgccatgc agattattgg ccgcctgaaa
    34141 cagcgggtgc atctggcgga cggcctgggg ccagataata tgttgagtga agaggcaatg
    34201 acgcgcggtt tgaactgtct gtcgctgttt gccgaacggc tacaagggtt ttctcctgcc
    34261 agcgtctgta tagtcggcac ccatacgctg cgtcaggcgc tgaacgccac tgactttctg
    34321 aaacgcgcgg aaaaggtcat tccctacccg attgaaatta tttccggtaa tgaagaagcc
    34381 cgtctgattt ttatgggcgt ggaacatacc caaccggaaa aaggtcgcaa actggttatt
    34441 gatattggcg gcggatctac ggaactggtg attggtgaaa atttcgaacc tattctcgtt
    34501 gaaagccgcc ggatgggttg tgtcagcttt gcccagcttt attttcctgg cggggtcatc
    34561 aataaagaga attttcagcg cgctcgcatg gcggcagcac aaaaactgga aactttaacc
    34621 tggcaattcc gtattcaggg ctggaacgtt gcaatgggcg cttccggtac cataaaagcc
    34681 gcccatgaag tgttaatgga aatgggcgag aaagacggga taattacccc ggaacgtctg
    34741 gaaaaactgg taaaagaaat tttacgtcac cgtaatttcg catcgctgag tttaccgggt
    34801 ctttccgaag agcggaaaac agtcttcgtt cctggactgg cgattttatg tggtgtgttt
    34861 gatgctttag ccatccgtga actgcgcctt tctgacgggg cgttacgcga aggcgtactg
    34921 tatgaaatgg aaggacgttt ccgtcatcag gatgtgcgta gtcgcaccgc cagcagcctc
    34981 gccaaccagt atcacataga cagcgaacag gcccgacgag tgctggatac cactatgcaa
    35041 atgtacgaac agtggcggga acagcaaccg aagctggcgc atccgcaact ggaggcgcta
    35101 ctgcgatggg ccgccatgct gcatgaggtc gggttgaata tcaaccacag cggtttgcat
    35161 cgccactccg cttatattct gcaaaacagt gacttgccgg gttttaatca ggaacagcag
    35221 ctgatgatgg cgacactggt gcgctatcac cgtaaagcga ttaagctcga cgatctgccg
    35281 cgctttacct tgtttaagaa gaaacagttc ctgccactga tacagctatt gcgccttggc
    35341 gtattactaa acaatcaacg tcaggcaacc accacaccgc caacattgac actgattact
    35401 gatgacagtc actggacact gcgtttcccg catgactggt ttagtcagaa tgcgctggta
    35461 ctgcttgatc tggaaaagga gcaagaatac tgggaaggcg tggctggctg gcggttgaaa
    35521 attgaagaag aaagtacccc agaaatcgcc gcttaatgct ttgcgggccg acgagaaatg
    35581 tcggcccgca ttattcaggc actttcgcga atgggttcga tttcattcag cgtatcaatt
    35641 aacggttgcg gcttaccaat aagataaccc tgcatataat cgatccccaa agagagcacc
    35701 gcctcgcgga tctcttcgtt ttcaacgtac tctgccacta cccgcatttt cttcattcgc
    35761 gccagatggc aaatcgacgc cacaatctga taatccagac tatttgacac aatattgcgg
    35821 ataaagctgc cgtcaatttt aagcagatcg gcattcacat ttttaagccg tgcatagctg
    35881 gcgtagccgg tgccgaaatc atcaatcgca atctggcagc ctaattcctg aagatgctgc
    35941 aaggtgattt gcgcctgctt aacattggtc agagcattac tttcggtgac ttcaaaaata
    36001 agttgccacg cttcaatctg atatttagcc agcaactgac tgacttcaac aggaaaacga
    36061 gcctgacata ccgaggttgg agacagatta atagcaaaac ggtgagcggg catcttcgct
    36121 ctgttttcag ccataaattg cagcgtatgc tcaatgaccc acatgtcgat actcgacgat
    36181 aaaccaaatt cgtgcgcgac cggcaagaag ctaccggggc cgatcagttc atcattctca
    36241 cctttcatgc gcagaagaat ttcatggtaa acatcaccac gcataccggt aatcggctgg
    36301 gccatcagga aaaaatggtt gtgttccagc gcctgctgta gtcgattcat catcgcgact
    36361 ttatctttca attcgcgttg caaatacatt gccccgcaac gctgcatatt ttccggggcg
    36421 ttggtcacga tggaaagttc ggcgaccgta tttagctctc ccagcagcag gtagatatga
    36481 ttcactggcg agcgcacata gcagtaactg acgccaatct gcggttgcat cggcatccca
    36541 tcccaaaaga aacgaaattg cttgagatga ctatccagtg cggtaatgcg ctcctggtgc
    36601 gattctgtat tcagtcgcag cgcgagatcg ttacccgaaa gctgataaac atcttcaccc
    36661 ggttccagca agggtgacag ccagtgagaa agtttttgct tgt
//
LOCUS       DAZPFP010000021        36587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPFP010000021 DAZPFP010000000
VERSION     DAZPFP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.021"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUR4_001019"
     CDS             <1..190
                     /locus_tag="ACHUR4_001019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454702.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHUR4_001020"
     CDS             complement(156..1391)
                     /locus_tag="ACHUR4_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1454703.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHUR4_001021"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHUR4_001021"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHUR4_001022"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHUR4_001022"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHUR4_001023"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHUR4_001023"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHUR4_001024"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHUR4_001024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHUR4_001025"
     CDS             complement(2077..3462)
                     /locus_tag="ACHUR4_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1454704.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUR4_001026"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUR4_001026"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1454705.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUR4_001027"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUR4_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1454706.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUR4_001028"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUR4_001028"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1454707.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUR4_001029"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUR4_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1454708.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUR4_001030"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUR4_001030"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1454709.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUR4_001031"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUR4_001031"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1454710.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUR4_001032"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUR4_001032"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1454711.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUR4_001033"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUR4_001033"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1454712.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUR4_001034"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUR4_001034"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1454713.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUR4_001035"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUR4_001035"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1454714.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUR4_001036"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUR4_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1454715.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUR4_001037"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUR4_001037"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1454716.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUR4_001038"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUR4_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1454717.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUR4_001039"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUR4_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1454718.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUR4_001040"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUR4_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1454719.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUR4_001041"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUR4_001041"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1454720.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUR4_001042"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUR4_001042"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1454721.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUR4_001043"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUR4_001043"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1454722.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUR4_001044"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUR4_001044"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1454723.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUR4_001045"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUR4_001045"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1454724.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUR4_001046"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUR4_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1454725.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUR4_001047"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUR4_001047"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1454726.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUR4_001048"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUR4_001048"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1454727.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUR4_001049"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUR4_001049"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1454728.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUR4_001050"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUR4_001050"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1454729.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUR4_001051"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUR4_001051"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1454730.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUR4_001052"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUR4_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1454731.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUR4_001053"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUR4_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1454732.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHUR4_001054"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHUR4_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUR4_001055"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUR4_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1454733.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUR4_001056"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUR4_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1454734.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHUR4_001057"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHUR4_001057"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHUR4_001058"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHUR4_001058"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9217 g         8419 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZPFP010000022        35798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPFP010000022 DAZPFP010000000
VERSION     DAZPFP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.022"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..677
                     /locus_tag="ACHUR4_001059"
     CDS             <1..677
                     /locus_tag="ACHUR4_001059"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1454735.1"
                     /translation="PLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGR
                     AVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGL
                     IVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNV
                     DLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFM
                     TARGYH"
     gene            complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHUR4_001060"
     CDS             complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHUR4_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1454736.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2517..4376)
                     /gene="gss"
                     /locus_tag="ACHUR4_001061"
     CDS             complement(2517..4376)
                     /gene="gss"
                     /locus_tag="ACHUR4_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1454737.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4581..5447
                     /gene="yghU"
                     /locus_tag="ACHUR4_001062"
     CDS             4581..5447
                     /gene="yghU"
                     /locus_tag="ACHUR4_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1454738.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5476..5557)
                     /locus_tag="ACHUR4_001063"
                     /pseudo
     CDS             complement(5476..5557)
                     /locus_tag="ACHUR4_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5570..5818)
                     /gene="hybG"
                     /locus_tag="ACHUR4_001064"
     CDS             complement(5570..5818)
                     /gene="hybG"
                     /locus_tag="ACHUR4_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1454739.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5952..6293)
                     /gene="hypA"
                     /locus_tag="ACHUR4_001065"
     CDS             complement(5952..6293)
                     /gene="hypA"
                     /locus_tag="ACHUR4_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1454740.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(6286..6774)
                     /gene="hybE"
                     /locus_tag="ACHUR4_001066"
     CDS             complement(6286..6774)
                     /gene="hybE"
                     /locus_tag="ACHUR4_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1454741.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(6767..7261)
                     /locus_tag="ACHUR4_001067"
     CDS             complement(6767..7261)
                     /locus_tag="ACHUR4_001067"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1454742.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(7261..8964)
                     /gene="hybC"
                     /locus_tag="ACHUR4_001068"
     CDS             complement(7261..8964)
                     /gene="hybC"
                     /locus_tag="ACHUR4_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1454743.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8961..10139)
                     /gene="hybB"
                     /locus_tag="ACHUR4_001069"
     CDS             complement(8961..10139)
                     /gene="hybB"
                     /locus_tag="ACHUR4_001069"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1454744.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(10129..11115)
                     /gene="hybA"
                     /locus_tag="ACHUR4_001070"
     CDS             complement(10129..11115)
                     /gene="hybA"
                     /locus_tag="ACHUR4_001070"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1454745.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(11118..12236)
                     /gene="hybO"
                     /locus_tag="ACHUR4_001071"
     CDS             complement(11118..12236)
                     /gene="hybO"
                     /locus_tag="ACHUR4_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1454746.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12425..12712)
                     /gene="yghW"
                     /locus_tag="ACHUR4_001072"
     CDS             complement(12425..12712)
                     /gene="yghW"
                     /locus_tag="ACHUR4_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1454747.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12831..13718)
                     /gene="yghX"
                     /locus_tag="ACHUR4_001073"
     CDS             complement(12831..13718)
                     /gene="yghX"
                     /locus_tag="ACHUR4_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1454748.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13875..14915
                     /gene="gpr"
                     /locus_tag="ACHUR4_001074"
     CDS             13875..14915
                     /gene="gpr"
                     /locus_tag="ACHUR4_001074"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1454749.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14955..15449)
                     /locus_tag="ACHUR4_001075"
     CDS             complement(14955..15449)
                     /locus_tag="ACHUR4_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1454750.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15640..16524
                     /gene="yghA"
                     /locus_tag="ACHUR4_001076"
     CDS             15640..16524
                     /gene="yghA"
                     /locus_tag="ACHUR4_001076"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1454751.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16796..17221)
                     /gene="exbD"
                     /locus_tag="ACHUR4_001077"
     CDS             complement(16796..17221)
                     /gene="exbD"
                     /locus_tag="ACHUR4_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1454752.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(17228..17962)
                     /gene="exbB"
                     /locus_tag="ACHUR4_001078"
     CDS             complement(17228..17962)
                     /gene="exbB"
                     /locus_tag="ACHUR4_001078"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1454753.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            18214..19401
                     /gene="metC"
                     /locus_tag="ACHUR4_001079"
     CDS             18214..19401
                     /gene="metC"
                     /locus_tag="ACHUR4_001079"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1454754.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19541..20200
                     /gene="yghB"
                     /locus_tag="ACHUR4_001080"
     CDS             19541..20200
                     /gene="yghB"
                     /locus_tag="ACHUR4_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1454755.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(20240..21139)
                     /gene="yqhC"
                     /locus_tag="ACHUR4_001081"
     CDS             complement(20240..21139)
                     /gene="yqhC"
                     /locus_tag="ACHUR4_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1454756.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            21333..22496
                     /gene="yqhD"
                     /locus_tag="ACHUR4_001082"
     CDS             21333..22496
                     /gene="yqhD"
                     /locus_tag="ACHUR4_001082"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1454757.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22601..23428
                     /gene="dkgA"
                     /locus_tag="ACHUR4_001083"
     CDS             22601..23428
                     /gene="dkgA"
                     /locus_tag="ACHUR4_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1454758.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            23628..24554
                     /locus_tag="ACHUR4_001084"
     CDS             23628..24554
                     /locus_tag="ACHUR4_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1454759.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24605..24862
                     /gene="yqhH"
                     /locus_tag="ACHUR4_001085"
     CDS             24605..24862
                     /gene="yqhH"
                     /locus_tag="ACHUR4_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1454760.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24905..27124)
                     /locus_tag="ACHUR4_001086"
     CDS             complement(24905..27124)
                     /locus_tag="ACHUR4_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1454761.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(27235..28647)
                     /gene="ftsP"
                     /locus_tag="ACHUR4_001087"
     CDS             complement(27235..28647)
                     /gene="ftsP"
                     /locus_tag="ACHUR4_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1454762.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28722..29459)
                     /gene="plsC"
                     /locus_tag="ACHUR4_001088"
                     /gene_synonym="parF"
     CDS             complement(28722..29459)
                     /gene="plsC"
                     /locus_tag="ACHUR4_001088"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1454763.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29693..31951)
                     /gene="parC"
                     /locus_tag="ACHUR4_001089"
     CDS             complement(29693..31951)
                     /gene="parC"
                     /locus_tag="ACHUR4_001089"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1454764.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(32497..32979)
                     /locus_tag="ACHUR4_001090"
     CDS             complement(32497..32979)
                     /locus_tag="ACHUR4_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1454765.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(33032..33424)
                     /gene="ygiW"
                     /locus_tag="ACHUR4_001091"
     CDS             complement(33032..33424)
                     /gene="ygiW"
                     /locus_tag="ACHUR4_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1454766.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33576..34235
                     /gene="qseB"
                     /locus_tag="ACHUR4_001092"
     CDS             33576..34235
                     /gene="qseB"
                     /locus_tag="ACHUR4_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1454767.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            34232..35506
                     /gene="qseC"
                     /locus_tag="ACHUR4_001093"
     CDS             34232..35506
                     /gene="qseC"
                     /locus_tag="ACHUR4_001093"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1454768.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(35552..>35798)
                     /locus_tag="ACHUR4_001094"
     CDS             complement(35552..>35798)
                     /locus_tag="ACHUR4_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR1454769.1"
                     /translation="NKYDWMLESGGNICSIPSVSGEDRILQAMIAAFFLLTPLIILIL
                     RKLFMREMFEFWLYVFSLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8886 a         9533 c         8980 g         8399 t
ORIGIN      
        1 ctccattaat tgccgttatt ggtagcgatg cttcaggcaa gtcaacggtg tgtgaacatc
       61 ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaggccg
      121 gaaatgtcgg tcgtgcagtg acaaaattac ctttgatggg aaaatcctta cataaaacaa
      181 ttgaacgaaa tcaggtgaaa acagcaaaaa aattgcctgg accagttccg gcgctggtaa
      241 ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc
      301 gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg
      361 gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg
      421 aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg
      481 ttgaccttga agttgcctgt gcacgtaaac ccgaccataa acgggaatcg ctggcgagga
      541 agattgccat aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca
      601 atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattacggat tttatgaccg
      661 cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag
      721 gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc
      781 ggtaaagtaa acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg
      841 ccgtccacca ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt ggagacaggc
      901 atcccaatat aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct
      961 tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc
     1021 cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacaccgga
     1081 gcgtactcaa tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct
     1141 ttactgacgc ctggcagttt cgccagcttc gcggaggtat cggagatgca cagcataatg
     1201 cggcgcagct ggctgcgctg actcacgctt aacggctcgt aactttccat attgcctggc
     1261 agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactct
     1321 gttacttgcg tgccatcagt cgatgctgca ggcaatggag gttccatcgc aatcaacttc
     1381 tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtaacggc atcgcgggta
     1441 cgggtaattt catagccgga cgcattcata ttgacgacga agccagcagg ggcaatcccc
     1501 accagtacca gcattaccag gccgatccct ttctgcccgt cgttcgcgcc gtgcgaaaac
     1561 gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg tttacgtttg
     1621 ccctttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtccca
     1681 ctccagaaac ggcgcagcag gaaaatcaga ccacccgcaa tgaccaggcc gacgataggg
     1741 gaaacaatca gcgaggagaa aattttggtt acttcacgca ggtttaacgc atccatcacc
     1801 gatgagccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg
     1861 tgcgaactgg aggccggtaa accaaagaac cacgttccca ggttccagat aatcgccgcc
     1921 agcagcatgg aaaagaccat cgccaaaccg tgagttgacc ccatattcag caacaaatcg
     1981 attggtaaca tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa
     2041 aagttaaaaa atgccgccat caccacagca agttgtggct gcatggcacg agtataaata
     2101 acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca
     2161 aatgccagag caagtaataa caaaagcccc gtatatatat caaggccaac aaataaattt
     2221 agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa
     2281 cggcatgggc aaagtgaaat atcatttttt aacgataaaa agagtgttat ttcttgtgta
     2341 tcagacaata agcattcatt caaaaccttt agaaataatc aaaacacagg tattccactg
     2401 gttaatatat tcacatatga aatgaataaa tattggaata tataaatatt gaatattttg
     2461 attaatcccc gtactaatta ttcttcataa tcagtacggg gttgctacaa catgtattac
     2521 tttttcacca caattaacgg ttcaatatca ctctcttttt tgatgaccag tgattcatca
     2581 ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat
     2641 ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat
     2701 ttaccgctgg ttttgtccag cacctcttca tgatggctga ccaggtcgat attgctacca
     2761 cagcgaccgg cgatcggttt cactgcgtaa cctgttttca ccaattcatc attaacagtg
     2821 aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc
     2881 gctttgttgc cggggatcac cgtccacagc ggctcaaaga ccagcacttc cgggcgcagc
     2941 aatacgtcga taagacgcac ttcgttttgc ggatgaccgg tacggattgg caccgcagca
     3001 aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc
     3061 cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc
     3121 agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc
     3181 ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga
     3241 cgtgcacgac tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg
     3301 aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc cgcttcggta
     3361 tgacaggagg cggagtcggc gttgtattcg taaaccttca ggccacgctc atccatgcag
     3421 aaatccatac gaccagtgat catatggtga cggcgacgct gccaggagag acgcaaacgt
     3481 ggccagagga ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg
     3541 tcggttgcgt gaagatacat cagatgcagc tcgttggtgg ctttaattag ctcctgctcg
     3601 gcactctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc
     3661 tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc aaactggcct
     3721 ttgttttcca gacgcgctcc actgattttc agcagctcgc ctgcaatttc cggctgcggt
     3781 aagctgtatt cagtatcttc cgtctggatc atccagccca gaatggtggt gtcatcaaaa
     3841 gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg cgtccactgt
     3901 tgcccttgcg gcaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca
     3961 tgcaattggg taatgatggc gacatggcca gtgtctttaa attcaccgcc tttatcccag
     4021 ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc
     4081 aggatgttgt cattaaccac ttctcgcagg aagcgcagcg agaaaatctc ccacgccata
     4141 cccacgtcag taaagaccac accgtaattc agaaagagaa aacggcgagc aaattcaacg
     4201 cattgccact tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct
     4261 tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg
     4321 gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact
     4381 ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc
     4441 agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca
     4501 aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac
     4561 cactcagaag gcaactcact atgacagaca atacttatca gcccgcgaaa gtctggacgt
     4621 gggataaatc cgctggcggc gcgttcgcca atatcaatcg cccgttttct ggtccgacgc
     4681 atgaaaaaac gctgcccgtt ggcaaacatc cattgcaact ttattcgctg ggaacgccga
     4741 acggtcagaa agtaacgatt atgcttgagg agctactggc gctgggcgtt actggtgcag
     4801 agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg
     4861 aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat ccgccgatcc
     4921 gcgtgtttga atctggttcg atcctgcttt atctggcgga gaaatttggc tacttcctgc
     4981 cgcaggatct ggcaaaacgg actgaaacgt tgaactggct gttctggtta cagggtgccg
     5041 cgccgttcct cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt
     5101 acgccatcaa ccgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac
     5161 tggcgcagca taagtttgtt gcgggcgatg agtacaccat tgcggatatg gcgatttggc
     5221 cgtggtttgg caacgtggtg ctcggcggcg tgtatgatgc cgcagagttt ctggatgcgg
     5281 gaagttataa acatgtgcaa cgctgggcga aagaagtggg cgaacgtccg gcagtgaaac
     5341 gtgggcgtat tgttaaccgc accaacgggc cgctgaacga gcagttgcat gagcgccatg
     5401 acgccagtga ttttgagaca aatacagaag ataagcgtca ggggtaaggg ttggtgttcg
     5461 tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa tcgtgcacaa
     5521 tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa agctaatcat cacgcgctgg
     5581 taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc tttggcttca tcttcgtcga
     5641 tgatgctcat ggcaaatccg acgtgtacca gcacccactg gcccagcaga tcggcagggt
     5701 taccttcgca aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg
     5761 caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg
     5821 ttaactccgg ttatttcgat tgccggacaa acgcgaaaaa gaacctttag cgggtgttcc
     5881 ctctcaccct aaccctctcc ccggaggggc gaggggaccg tccgtgcgct ttttaggcgt
     5941 tgtcctcagt cttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca
     6001 cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc
     6061 tggctgcaat cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct
     6121 tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg
     6181 caggagagcg cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc
     6241 gcctgccgtt ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc
     6301 cctcctgcgg ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattggtgac
     6361 tggcaacgaa aggatcattc gtgcgcagtc atccgtcagg cgttggcctt cttcaatcga
     6421 catgctgtgc gaaagcggcg acatcagcga acaggagagg tattgcgaaa caccgtccag
     6481 ttcgccaacg gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac
     6541 tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg
     6601 ggtgatcaca caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg
     6661 cattgaagga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc
     6721 tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg
     6781 cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg
     6841 caggttcaat cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg
     6901 ggatcacgcc gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca
     6961 atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt
     7021 cttcatcccg caggatcatc atcgttcccg gagtgttctt tttcgacaca atggcatccg
     7081 caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc
     7141 catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc
     7201 gcacgccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca
     7261 ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca
     7321 ggccatgcac gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc
     7381 ggcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg
     7441 cggaccagag ttccaggttg atggaacaac cgcctggtag ttgctgatga taccgtcttt
     7501 aataaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa
     7561 ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt tgccgatatt
     7621 ggtgatcagt gcactgtatt ggttttgcag gatatcctgc aattcacagc agtgaacggt
     7681 acgaccaata atacggccca gcgtggagtg cagctgtgcc acttccagcg tgttgccagt
     7741 cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc
     7801 cgccagtttc accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt
     7861 cggtgatttc acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt
     7921 ggtaccttcc cacggcgcct gtggcgcttc gtctttatac caggagtgct tcgcgctttc
     7981 ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag
     8041 atccgcattc tcaatgtagc ctcccgggaa caggaagctg ccgtttttgc tgtcggtcgg
     8101 gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta
     8161 gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc tcaacaaagt cgctcagttt
     8221 gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc aacacgccca acccgtcgag
     8281 gttgattggg ttcgcgacac cacccacagc caggttctga atgtgcggcg ttttaccgcc
     8341 cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc
     8401 taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt
     8461 ggcgaaaata cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt
     8521 gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt
     8581 ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata
     8641 gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg
     8701 cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc
     8761 gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc
     8821 gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga
     8881 aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg
     8941 atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt
     9001 tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata
     9061 gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg
     9121 cccgggttga atgccaccaa cgaataggtc agacgccagg ttgcacaacc taacagtgcg
     9181 ctcagtgctg acaggaacag catacgggaa tcattacgca gcttcgccac acgcagaacg
     9241 accagcggga agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg
     9301 gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag cccgatgaaa
     9361 atcgccagca acacactgat ggtgttggtc agcttgacga acaggctctt ttcatccgga
     9421 ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa agatgacaat cgagaagccc
     9481 atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg ccagagcgga
     9541 tgcaccttgt agcccgccga gatcatcagc gaccccattg aagactggtg catggtcggc
     9601 agcagcgcac cgagcgcgat gatgaagaac atcaccttgt tcagacgttg tagcgacacc
     9661 ttccagccca gacgttcaaa cagtgccgga gcaaactcca gagccatcac gccgatgtag
     9721 atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg
     9781 tagaagtacg gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt
     9841 gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg
     9901 ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccaatc
     9961 agcaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag
    10021 cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca
    10081 aaaatcatga ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg
    10141 atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc
    10201 cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga
    10261 acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc
    10321 cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata
    10381 tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta
    10441 ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt
    10501 accgaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc
    10561 gagacgttcc acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt
    10621 gttgtagtcg tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt
    10681 gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac
    10741 cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc
    10801 gtagccgttc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat
    10861 gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt
    10921 acgttcaggg aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa
    10981 ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc
    11041 atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa
    11101 attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga
    11161 cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc
    11221 aaaccaatag cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt
    11281 gaacgcggag tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata
    11341 cctttttcgt tacagccata gcacgggtga ccaatcgcca ccggccacac accgccaaca
    11401 tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg
    11461 tacaagcacc agccttcgcg gtggccttca tcaccgaact ctttggcaaa acgaccagca
    11521 tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga
    11581 cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg
    11641 aggaagttgt gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga
    11701 acttcttgca agctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac
    11761 gcggagcagg aaccaatagc gataatggct gctgagcctt ccgccgcttt gcggatgtga
    11821 tccacaatcg gctcaccggc aaccatgcaa taaataccgt tatcttttaa tgggatggaa
    11881 ccatccacca ctaacacata ctgccctttg tacttctcga gagcgttatg tttgttctct
    11941 tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat attccagaga gatagtctcc
    12001 agcacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg
    12061 cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc
    12121 atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc
    12181 atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat
    12241 agttattccg ttgcgagaac ctggcatata ttttgcctca atcgcaaaat caataatgcg
    12301 atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg
    12361 gcgctaaaac gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt
    12421 ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat
    12481 tctcacgaaa gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac
    12541 ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatcctg tgtgagtagc
    12601 ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg
    12661 catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc
    12721 tcattcatta tatatcgcca tatataacga tattttaggc aaagaacgac ttttgcgcac
    12781 gctatttttt catacttgtt taagacgtaa tcccgtaaga gataaaccta tcaggagaga
    12841 tatttatcga accatttcag tgtcctttgc caggcaagat cggcggcaga tttgtcataa
    12901 cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatagatata agcctcataa
    12961 accttattat tgtctttcaa cgccgcctcg taaggaggcc agccctcgtt gattcgggtg
    13021 tccagttccg cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt
    13081 gcctgacgac cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg
    13141 ttcgatacgc caccgccata gcaaaatccg gtaataccca ctttacctgt cgcttgcgga
    13201 tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc
    13261 tgctgttgca gctcacgacc tttatcatca tttcccggat aacctccaac ggaacttaat
    13321 ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata
    13381 tacggattca gtccacgatt ctcatgcacc acccccacgg ctggcgtttt gccgctcatt
    13441 tttgcgggtt tcaccagata accccgtacc tcgccgtgac catttggcga aggatacgtg
    13501 atgtactcag caacaatctc cgggtcggta aactctacct gagtcgccag cgcataattt
    13561 ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca
    13621 aggttgagga actcacgttt cgaaattttc ccgtgaacgt aatagtcgta gtaatccaac
    13681 aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg ttgcattcct caactggtgt
    13741 ttttttaagc aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag
    13801 actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt
    13861 ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcagtacc
    13921 gctattgcgg aaaaagtggt ttacgcctgc ccgcattatc gctcggttta tggcacaatt
    13981 tcggtcacgt taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg tttgatttag
    14041 gcattacgca ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga
    14101 actttggtcg cctgctgcgc gaggattttg ctgcttatcg cgatgaactg attatctcta
    14161 ccaaggctgg ctacgatatg tggcccggcc cgtacggctc tggcggttca cgtaaatacc
    14221 tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt
    14281 actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg
    14341 cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc
    14401 aaaaaatggt cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt
    14461 acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg
    14521 gcgtgggctg tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca
    14581 acggcattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc
    14641 cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc
    14701 agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga
    14761 cgtcggtatt gattggtgcc agccgcgcgg agcaactaga ggagaacgtg caggcgctga
    14821 ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg
    14881 agctgaatct gtggcaggcg tcgtccgata aatgacctgt taataacggg cgcgcgaacc
    14941 gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat
    15001 gcagaaagca caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca
    15061 tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct
    15121 gctactttgt ttttcagcga cgttgcgtcc attttcccca gccacttcag cttctctttg
    15181 ttctcggaga tatccagctg tgagacgaaa ttctcataac cggaaaacat caccatcacc
    15241 agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat
    15301 tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc
    15361 agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca
    15421 tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac
    15481 agtatatcgc aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt
    15541 gtcgcataat tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt
    15601 ctacccttaa aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg
    15661 accacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag
    15721 gcgaagatga caccgatacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg
    15781 aaagatcgta aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc
    15841 gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac
    15901 gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc
    15961 gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc
    16021 gggctggata ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac
    16081 ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta
    16141 acccaggaag cgatccccct gctaccgaaa ggtgcaagtg tcatcaccac ttcgtcaatc
    16201 caggcatacc agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg
    16261 aactacagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc
    16321 gcgccaggcc ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc
    16381 ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct
    16441 gtatatgttt atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg
    16501 tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat
    16561 tgtgaattca ctcttacctg atgtggcgaa aacgccttat ccgcctacaa acgcatgaag
    16621 attcaacatg ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc
    16681 acctaatgcc ggatgcgtcg tgaacgcctt atccggccta caaacgcacg aagattcaac
    16741 atgttgcaat agattttgta ggcctgatga gcgtagcgca tcaggcaatt ctactttact
    16801 tcgctttggc ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg
    16861 tatccattac cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg
    16921 tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga
    16981 ccggatcgtt accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt
    17041 ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg
    17101 ctaacggtgc cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg
    17161 tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca
    17221 tcggacatta tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg
    17281 cttccagatc taggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca
    17341 tcgctttaaa gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg
    17401 ctgccacgag gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg
    17461 ccaggttagt ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg
    17521 taccaaacag accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc
    17581 ggcccatttg acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt
    17641 taataccttc gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga
    17701 gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta
    17761 aggaacgtgc ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga
    17821 actctacgct cttactgaag aagattgccc aggtgactac agaggccaaa ataagcccaa
    17881 tcatcacgca cttaacgaca atatcggcgt actgatacat accccagacg gaaaggtccg
    17941 tctgcattaa attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat
    18001 aataatatca aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt
    18061 acctttgctt tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg
    18121 aaaattttct gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta
    18181 gacatccaga cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa
    18241 ctggtgaatg caggacgcag caaaaaatac actcttggcg cggtaaatag cgtgattcag
    18301 cgcgcttctt cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc
    18361 gccaatggcg agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa
    18421 gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg
    18481 gttgctaatt cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc
    18541 gcctatgaac cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca
    18601 tcgtggtttg atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa
    18661 atcgtgtttc tggaatcgcc gggttccatc actatggaag tccacgacgt tccggcgatt
    18721 gttgccgccg tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca
    18781 gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg tttctattca ggccgccacc
    18841 aaatatctgg ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc
    18901 tgggagcagc tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc
    18961 tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc
    19021 agtctgaaag tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct
    19081 gccctgcctg gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg
    19141 ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat
    19201 aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca
    19261 aatcaaccag aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc
    19321 ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc
    19381 ggttttgcac ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt
    19441 gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat
    19501 aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat
    19561 catcgctgcg ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt
    19621 tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt
    19681 tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga
    19741 ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta
    19801 tattcagggg agctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc
    19861 tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct
    19921 ggctggacgt tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg
    19981 tctgccaaac cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt
    20041 ggtaaccagt tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca
    20101 ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac
    20161 gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc
    20221 tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc
    20281 ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc
    20341 cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct
    20401 tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat
    20461 tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt
    20521 tttcggtgta tttattctca attcgtttca gcacgcggct aatcagactg aagtgagtct
    20581 ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa
    20641 tttcgcggat gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga
    20701 gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg
    20761 ctgccatcga cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact
    20821 gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt
    20881 cgcactcaaa cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca
    20941 cgcgttcatt gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt
    21001 acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca
    21061 gttctgacaa actattttct ttatttttca gtttattaac tttatccgcc agcaagcgac
    21121 aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg
    21181 cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg
    21241 agcattgaga gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg
    21301 ccctcatatt ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa
    21361 cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg
    21421 ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag
    21481 ttctggatgc cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg
    21541 cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc
    21601 tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact
    21661 atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg
    21721 ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg
    21781 cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca catgttcagc
    21841 cggtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta
    21901 acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg
    21961 ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa gatggtccga
    22021 aagccctgaa agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgactc
    22081 aggcgctgaa cggtttgatt ggcactggcg taccgcagga ctgggcaacg catatgctgg
    22141 gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc
    22201 ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac
    22261 gcgtctggaa catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa
    22321 cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg
    22381 gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg
    22441 aaaatcatga cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt
    22501 tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca
    22561 ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat
    22621 taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa
    22681 tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac
    22741 cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa
    22801 cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga
    22861 agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca
    22921 ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca
    22981 aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg
    23041 cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat
    23101 gcaacaacgc cagctacacg cttggaacgc gacacacaaa atccagaccg aatcctggag
    23161 cccattagcg caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga
    23221 taaatacggc aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt
    23281 ggtgatcccg aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt
    23341 ccgtctcgac aaagacgaac tcggcaaaat tgcaaaactc gatcagggca agcgtctcgg
    23401 tcccgatcct gaccagttcg gcggctaacg tgcaaattct cccggtggcg gtaatgttcc
    23461 gctaccggac ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc
    23521 ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa
    23581 caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac
    23641 ttctgttttt agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg
    23701 tagaacaaac agtccggcag atttatcaga actataaatc agatgccagt acgccttatt
    23761 tcggtgaaac cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga
    23821 acgacaatct tacactaccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt
    23881 gtcaggattt tggcgatctg gtgttagaaa gcgtagcgat aacccaaact gacgtcgatc
    23941 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacacaga
    24001 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga tgatattgtc agcaatcatg
    24061 gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
    24121 tgcagaaaga acagccagaa tcctttgttg ctgaactctt tgaacatatt gctgattata
    24181 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata
    24241 aaaccacctt caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta
    24301 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
    24361 gagtcctgga gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg
    24421 gcaacaatga agagcaagag ctggttttac agcggcgcga aggtaagtgg gaaatcgctg
    24481 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
    24541 gattaaagca atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca
    24601 ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg
    24661 ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat taaatgccaa
    24721 aatcgcccgg cttgagcagg atatgaaagc actacgccca caaatctatg ctgccaaatc
    24781 cgaagctaac agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg
    24841 cttgcgtatg tacgcagaat gataaaaaaa tccccggcag catgtcagtt gccggggatt
    24901 ttttttaacg tccaaccgcc gctttagggc gtttcttcgc accagcattc accggacgag
    24961 attgcgtaga cgacgctttt tttgccgtag caggcgtctg acgctgagtc gccatcggcg
    25021 tatgtttagt caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt
    25081 caatggttgg cgcaggcact aagcaatcgc gacggctgcc aatcagatgc tttttgccca
    25141 tcgcttccag cgcctggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg
    25201 ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg ctcttataac
    25261 caatcttcgc cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg
    25321 atgggtagaa gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca
    25381 aattcaccat atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac gggatcagat
    25441 actgctcttt acctgcctgt ttcgagtagg tatcgaacag ctctttaaag cggtcatagc
    25501 tgcccatgcc cggcttcatc atcttcgaca acggcccttc ttcggtatgt tccggagcaa
    25561 tcttcagata accgccgacg tgatgggtcg ccaactcttt gatatagcgc ggatcttcca
    25621 cggcgatgtc ataacgtacg cctgaggcga taaggatttt tttaatgcct ttcagatcac
    25681 gcgcacggcg atagaggttg atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat
    25741 ccggataaac gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat ttgcagcgca
    25801 acatatacat gttggcagtt ggcccaccga gatcggaaat cacgcccgta aaacctggaa
    25861 cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg ctctgaataa
    25921 tgcgcccttc gtgctcggta atagaacaga aagaacagcc gccaaagcag ccgcgcataa
    25981 tgttgaccga aaaacggatc atttcgtaag ccggaatacg ggcattgcca taggccggat
    26041 gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga
    26101 tagcaggcgg gttgatccac acatagcggt caccgtgttt ttgcatcaat gcgcgggcgc
    26161 agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc actttatcgc
    26221 ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt ttctggcgcg
    26281 gtggctgcac gattacagct ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg
    26341 cacacggcaa atcttcgcca tacggatgcg ggattgggtc gattttccct agggtatcaa
    26401 gacgggtgga atccacaccg ctccagccag gcagcgcctc ttttacgata attgcggtat
    26461 tacgcacatc gcggatttca ctaatcggct cgcccatcgc cagacgatgc gctacttcca
    26521 ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct accagaacgg
    26581 aacggcgcac ggtatcggac cagtaatcat aatgcgcggt acggcgcagg ctagcctcaa
    26641 taccgccgag gatcaccggc acatctttcc acgcctcttt acaacgctgg gtataaacca
    26701 gcgtggcgcg atccggtcgc ttacccgcga cattatctgg cgtgtaggca tcatcatgac
    26761 gtaaacggcg atcggcggta tagcggttga tcatcgaatc catgttgcca gcagtaacac
    26821 caaaaaacag attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct
    26881 gggcgatgat cccgacgcga aagccctgcg cttccagcat acgaccgcaa atcgccatcc
    26941 cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc
    27001 agccaagttg atccatctct tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg
    27061 cggcccagta ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc
    27121 tcattttgct tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac
    27181 gaaagagaaa tcgaaaggta ttccatactc gccctcctcg ggcgagtatg aagattacgg
    27241 taccggattg accaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact
    27301 gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac
    27361 ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcgcc
    27421 attcacgtta cggatctgga acattacgcc ttcaatatgg aacgcttgcg gctcgtccgc
    27481 gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc
    27541 ccacagctgt ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaattgg
    27601 cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg
    27661 cagaaggccg gttgggcgca gcgttagcac caaggtagaa accagaatac tggatggctc
    27721 aaagaagccg cgaatacgat caacaatgct cgccgcttca ccacaggtga tcgacacttc
    27781 atcgccgttg ctcatatcca ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg
    27841 cttcaccgat acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg
    27901 accatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg
    27961 cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc
    28021 accaacaaag ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg
    28081 tttatcctgg ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga
    28141 cttgctgact tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc
    28201 cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc gaataggcag
    28261 tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc
    28321 tggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat
    28381 aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat
    28441 tccccagacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat
    28501 aaacagcggt tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg
    28561 cccggctgcg ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg
    28621 aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac
    28681 cgtgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct
    28741 tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa
    28801 tgggcagcca gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc
    28861 atttcgacaa tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta
    28921 gagacgcaca cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc
    28981 ttgaacggta gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa
    29041 atacggcgtt ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga
    29101 ttgtttctgt cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc
    29161 cacagcaagc tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc
    29221 accatgtcat agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca
    29281 tctgcaggtt tgcggcactc aactttcagg ccgaacagtg gcgcaagacg gccaaacata
    29341 tgcccaaagg tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg
    29401 aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata
    29461 acaccctctg aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat
    29521 tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg
    29581 tttcaaggga atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg
    29641 tttcatccgg cgttccttgc tagcgggagg aaacagcgcc ctccccggca tattactctt
    29701 cgctatcacc gctgctggca cggcgaggag agtcgatctc aacacgatcg atacgctgca
    29761 aaccgcgcat caacgtaccg cggcgtccac gttcgccagt gactttctgt agctcttccg
    29821 gacgcagttt aattttgcgt ttcccaacat gaatggtcag cgtgctttgc ggcggcagaa
    29881 cgtacagttg cgccagacca tcctcgccac gcgcggcttc tgccgatgga atgttgataa
    29941 tcttgttgcc tttgcccttc gacagctgcg gcagatcgct taccgggaac atcaacatac
    30001 ggcctgcctg agtgattgcc agcagcatat cggaagcatc ttcaatcacc accggcggca
    30061 taacatgggc attttccggt aaggtgatca aagccttacc cgcacggtta cgcgctacca
    30121 gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc agtttctgat
    30181 cgtcgctttc catcagcata tggtcaacgg tcgccccagg cggcaacgtt aatttgccgg
    30241 tgagtggttc gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg gcatagctac
    30301 gaccggtgga atcaacaaac actaccggtt ggttactctt acctttcacc gccgctttga
    30361 agctatcacc cgctttataa ttcaggcccg gcgcgtcgat atcatggcct ttagcgctgc
    30421 gtacccagcc catctgcgac agcacaatgg tgacaggttc agacggcagc atgtcgtgct
    30481 cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga acgacgatcg tcaccgtagg
    30541 cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt cattttacgc tcggaagcca
    30601 aaatgccctg caactggtcg cgctcttttt ccagctcact ctgctcaccg cgaatcttca
    30661 tctcttccag tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc gcctgggttt
    30721 ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc atcttcgtta cgaatgatct
    30781 caatcacttc gtcgatgttg agaaacgcca ccagcaaacc ttccaggata tgcaggcgct
    30841 tgaggacttt ctccagacga tagttcagtc ggcggcgcac ggtatcgcga cggaacacca
    30901 gccattcgga gaggatttcc agcaggtttt tcaccgccgg acgaccatcc agaccgatca
    30961 tattgaggtt aatgcgatag ctcttttcca gatcggtggt agcgaagagg tggttcatca
    31021 cctgatccat atccacgcgg ttggaacgcg gcacaatcac cagacgggtc gggttctcgt
    31081 ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc atttgcgcag
    31141 caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg ataaccaccg
    31201 cgccatcttc tttcttccac accgcgcgca tacgcactga accacgtccg ttctcgtaga
    31261 ttttacgaat ctcggcgcgc gaagtgataa tttccgcttc agtcggataa tccggcccct
    31321 gcacgatatc cagcagctga tcgagcgtag ttttcggctg gtcgattaat gcgattgccg
    31381 cctgagctac ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg acggcaatac
    31441 cggtggtgcc gttaagcaaa atgtttggca gacgggcagg tagcattttc ggctcctgca
    31501 aagtgccgtc gaagtttggc acccagtcag ccgtcccctg ccccagctcg ctcaatagca
    31561 gctcggaata tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac gatttcggat
    31621 cgtccggcgc gccccagttc ccctgaccat caaccagcgg ataacggtaa gagaacggct
    31681 gcgccatcag gaccatcgct tcataacagg cgctatcgcc gtgcggatgg tatttaccca
    31741 gtacgtcacc gacggtacgg gccgattttt taaatttggc gctggcattc aggcccagtt
    31801 cagacatcgc atacacaatg cggcgctgaa caggtttcag accatcacca ataaacggca
    31861 acgcacggtc catgatcacg tacatggagt agtttaagta ggcgttttcc gtaaattcat
    31921 gtagcgcaag gcgctctgcc atatcgctca ttaattctga ttcctcaact tattcgccag
    31981 ccttcaaagg ggcaatattg ccggagatac tacattatct gccgcgtcga gtcacaggag
    32041 atcagcgtga atagcgccgc cggacgtgct ccgacggcga taaaggcatc aatgtgcctt
    32101 gatatacaac tctttgctgt agacttgatc ggcatagagg gcagcacaag gttgcgccca
    32161 tgccacacca ctcggcatgg gggtccgctc cgggcgattc gagaagttga ggttatgcga
    32221 gtccagggaa gcctaaagca tcaaagtagt cgactgtaag tatacggttc agcccagttt
    32281 agctgtgtct ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc tcatggatat
    32341 ccccgagacg gcttacggcg caccggataa taaatttcgc gtatacatgg tattccttac
    32401 cggagaaatt ataagcccaa caacaatacg cctgtgacat tgttcgctca aacttatcgc
    32461 gagtgatttg gctcacactc cggtaaagat tccccatcac gccaacggca cataaacatc
    32521 cgtttccagt cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa acagaatcgg
    32581 tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaataatgc cccatatcgt
    32641 gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg gcataacgtc caccggtaag
    32701 ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc tgccgcagat
    32761 atcaaagcga aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga cgccaaaagt
    32821 ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa atttcgccac
    32881 actgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag ggattgatgg
    32941 gaaatcgata atgtttacat ccagtgtcag gtttgtcatt ttgcctccct gcgaaattat
    33001 tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg atctgtttga
    33061 cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca accgtatctt
    33121 tcggcgtcac cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg atagtaccgc
    33181 tggcatcttt aaacacgtag agatcgtcag agatgcgttc aacgatattg ccgcgcaggg
    33241 ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg ctgccgttcg
    33301 gcccctgaaa tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa ccgccctgct
    33361 ctgctgccat caccggtgca ctgcacaggg ccattactgc gattactgct gcgaattttt
    33421 tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata gtccttaaca
    33481 acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca acacggttta
    33541 ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg atagaagatg
    33601 acatgctgat tggtgacggc atcaaaactg gccttagtaa aatgggtttt agtgtcgact
    33661 ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat gatgcggtga
    33721 tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa tggcgagaga
    33781 aaggtcagcg tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg gaacgtgtag
    33841 aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg atagaagtcg
    33901 ccgccaggct ggaggctctg atgcgccgaa ccaacggcca ggccagcaac gagctgcgcc
    33961 acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc gaacccttaa
    34021 cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct ggtcgggtac
    34081 tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc accagtaatg
    34141 ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt attcgtaccg
    34201 tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc ttagtctgcg
    34261 cgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc tttccagctt
    34321 tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc aactgatgct
    34381 gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg atcgcatggc
    34441 acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga cctttgccat
    34501 ctttacccac gacggcagaa tggtccttaa tgatggcgat aacggagaag atattcccta
    34561 tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg atccttggcg
    34621 ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggcc aggaatggga
    34681 ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt ggctggtcgc
    34741 actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg cgccgctgaa
    34801 caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac taaacgcgac
    34861 tggcgtaccc agcgaagtgc ggccactggt tgagtcgcta aatcaactgt tcgcccgcac
    34921 acatgcgatg atggttcgag aacgacgctt tacctccgat gcagctcacg aactacgtag
    34981 cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg atgatccgca
    35041 ggcgcggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta ctcgtctggt
    35101 tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg acgtcgcgga
    35161 gatcccgctt gaagatctcc tacaatcgtc ggtgatggat atttaccaca cggcgcagca
    35221 ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc atcaaacgca ccggacaacc
    35281 gctattgcta agtttgttgg tgcgaaattt gctggataac ggccccggtg tgacaccaga
    35341 ggcactggcg cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg ctaccggcag
    35401 tggacttggg ctatcgattg tccagcgaat cgccaagctg catgacatga atgttgagtt
    35461 tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc tctgtctatg
    35521 agagccgtta aaacgactct catagatttt actaatagca aaatataaac cgtccccaaa
    35581 aaagccacca accacaaacc agacaaataa cgagcgagaa tacatatagc caaaactcga
    35641 acatttcccg cataaagagt tttcttaaga taagaataat aagtggcgta agaagaaaaa
    35701 atgctgcaat cattgcttgc aatatacgat cttcgcctga aacggaaggt atactgcaaa
    35761 tattgcctcc cgactcaagc atccagtcat acttattg
//
LOCUS       DAZPFP010000023        35557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPFP010000023 DAZPFP010000000
VERSION     DAZPFP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.023"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            11..247
                     /locus_tag="ACHUR4_001095"
     CDS             11..247
                     /locus_tag="ACHUR4_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1454770.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            250..1107
                     /locus_tag="ACHUR4_001096"
     CDS             250..1107
                     /locus_tag="ACHUR4_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1454771.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1107..3119
                     /locus_tag="ACHUR4_001097"
                     /pseudo
     CDS             1107..3119
                     /locus_tag="ACHUR4_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(3120..3641)
                     /gene="sodC"
                     /locus_tag="ACHUR4_001098"
     CDS             complement(3120..3641)
                     /gene="sodC"
                     /locus_tag="ACHUR4_001098"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1454772.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3722..4618)
                     /locus_tag="ACHUR4_001099"
     CDS             complement(3722..4618)
                     /locus_tag="ACHUR4_001099"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1454773.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4667..4906)
                     /locus_tag="ACHUR4_001100"
     CDS             complement(4667..4906)
                     /locus_tag="ACHUR4_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1454774.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5009..5608
                     /gene="nemR"
                     /locus_tag="ACHUR4_001101"
     CDS             5009..5608
                     /gene="nemR"
                     /locus_tag="ACHUR4_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1454775.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5645..6742
                     /gene="nemA"
                     /locus_tag="ACHUR4_001102"
     CDS             5645..6742
                     /gene="nemA"
                     /locus_tag="ACHUR4_001102"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1454776.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6823..7230
                     /gene="gloA"
                     /locus_tag="ACHUR4_001103"
     CDS             6823..7230
                     /gene="gloA"
                     /locus_tag="ACHUR4_001103"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1454777.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7333..7980
                     /gene="rnt"
                     /locus_tag="ACHUR4_001104"
     CDS             7333..7980
                     /gene="rnt"
                     /locus_tag="ACHUR4_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1454778.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8073..12531
                     /locus_tag="ACHUR4_001105"
                     /pseudo
     CDS             8073..12531
                     /locus_tag="ACHUR4_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(12575..13783)
                     /locus_tag="ACHUR4_001106"
     CDS             complement(12575..13783)
                     /locus_tag="ACHUR4_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1454779.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            13806..14216
                     /gene="tnpA"
                     /locus_tag="ACHUR4_001107"
                     /pseudo
     CDS             13806..14216
                     /gene="tnpA"
                     /locus_tag="ACHUR4_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(14330..14677)
                     /gene="grxD"
                     /locus_tag="ACHUR4_001108"
     CDS             complement(14330..14677)
                     /gene="grxD"
                     /locus_tag="ACHUR4_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1454780.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            15012..15839
                     /gene="mepH"
                     /locus_tag="ACHUR4_001109"
     CDS             15012..15839
                     /gene="mepH"
                     /locus_tag="ACHUR4_001109"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1454781.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15967..16548
                     /gene="sodB"
                     /locus_tag="ACHUR4_001110"
     CDS             15967..16548
                     /gene="sodB"
                     /locus_tag="ACHUR4_001110"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1454782.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16694..17863)
                     /locus_tag="ACHUR4_001111"
     CDS             complement(16694..17863)
                     /locus_tag="ACHUR4_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454783.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18029..18118)
                     /gene="cydH"
                     /locus_tag="ACHUR4_001112"
                     /gene_synonym="ynhF"
     CDS             complement(18029..18118)
                     /gene="cydH"
                     /locus_tag="ACHUR4_001112"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1454784.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            18417..19442
                     /gene="purR"
                     /locus_tag="ACHUR4_001113"
     CDS             18417..19442
                     /gene="purR"
                     /locus_tag="ACHUR4_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1454785.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19439..20371)
                     /gene="punR"
                     /locus_tag="ACHUR4_001114"
     CDS             complement(19439..20371)
                     /gene="punR"
                     /locus_tag="ACHUR4_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1454786.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            20484..21695
                     /gene="punC"
                     /locus_tag="ACHUR4_001115"
     CDS             20484..21695
                     /gene="punC"
                     /locus_tag="ACHUR4_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1454787.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            21986..23134
                     /gene="cfa"
                     /locus_tag="ACHUR4_001116"
     CDS             21986..23134
                     /gene="cfa"
                     /locus_tag="ACHUR4_001116"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1454788.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(23174..23815)
                     /gene="ribE"
                     /locus_tag="ACHUR4_001117"
     CDS             complement(23174..23815)
                     /gene="ribE"
                     /locus_tag="ACHUR4_001117"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1454789.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            24030..25403
                     /gene="mdtK"
                     /locus_tag="ACHUR4_001118"
     CDS             24030..25403
                     /gene="mdtK"
                     /locus_tag="ACHUR4_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1454790.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(25444..26700)
                     /locus_tag="ACHUR4_001119"
     CDS             complement(25444..26700)
                     /locus_tag="ACHUR4_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1454791.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            27008..27084
                     /locus_tag="ACHUR4_001120"
     tRNA            27008..27084
                     /locus_tag="ACHUR4_001120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27042..27044,aa:Val,seq:gac)
     gene            27089..27165
                     /locus_tag="ACHUR4_001121"
     tRNA            27089..27165
                     /locus_tag="ACHUR4_001121"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27123..27125,aa:Val,seq:gac)
     gene            27273..27578
                     /locus_tag="ACHUR4_001122"
     CDS             27273..27578
                     /locus_tag="ACHUR4_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1454792.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            27704..29126
                     /locus_tag="ACHUR4_001123"
                     /pseudo
     CDS             27704..29126
                     /locus_tag="ACHUR4_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(29138..29950)
                     /gene="ydhT"
                     /locus_tag="ACHUR4_001124"
     CDS             complement(29138..29950)
                     /gene="ydhT"
                     /locus_tag="ACHUR4_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1454793.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(29954..30739)
                     /gene="phsC"
                     /locus_tag="ACHUR4_001125"
     CDS             complement(29954..30739)
                     /gene="phsC"
                     /locus_tag="ACHUR4_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1454794.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(30736..31404)
                     /locus_tag="ACHUR4_001126"
     CDS             complement(30736..31404)
                     /locus_tag="ACHUR4_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1454795.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(31468..32106)
                     /locus_tag="ACHUR4_001127"
     CDS             complement(31468..32106)
                     /locus_tag="ACHUR4_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1454796.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(32119..34220)
                     /locus_tag="ACHUR4_001128"
                     /pseudo
     CDS             complement(32119..34220)
                     /locus_tag="ACHUR4_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(34241..34867)
                     /locus_tag="ACHUR4_001129"
     CDS             complement(34241..34867)
                     /locus_tag="ACHUR4_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1454797.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(35323..35532)
                     /gene="fumD"
                     /locus_tag="ACHUR4_001130"
     CDS             complement(35323..35532)
                     /gene="fumD"
                     /locus_tag="ACHUR4_001130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1454798.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         8539 a         9339 c         8710 g         8969 t
ORIGIN      
        1 aatctacttt gtgaagttta tgctcaatgc aacaggattg cccttacaag atctggtgtt
       61 cggtgcgtcc gtctattttc ctccgttttt caaagcattc gcgtttggat tcgtcatctg
      121 gcttgtcgta caccgcctgc ttcgtggctg gatctacgcc ggcgatatct ggcatccctt
      181 gttaatggat ttatcgctgt ttgcgatttg cgtttgcctt gctctggcaa tactgattgc
      241 gtggtaacta tgtcaattaa aacaattaag tatttctcaa caatcattgt agcgatagtt
      301 gcggttcttg ccggatggtg gctgtggaat tattacatgc aatctccgtg gacacgcgac
      361 gggaaaatac gcgcagaaca ggtttctatc accccacagg tgtcgggacg tattgttgag
      421 ctgaatataa aagacaacca actggttaac gcgggggaac ttctgctcac gatcgataaa
      481 acgccctttc agatcgccga gctgaacgca caggctcagc tggcgaaagc gcaatctgac
      541 ctcgcaaaag ccaataacga agcgaatcgt cgtcgtcatc tctcacaaaa ttttattttt
      601 gccgaagaac tcgataccgc taacctcaat gttaaagcga tgcaggccag tgttaatgcc
      661 gcacaggcga cgctgaaaca gacacaatgg caactggcgc aaacggaaat tcgcgccccg
      721 gtgagtggat gggtgactaa cctcactacc cgcatcggcg attacgctga caccgggaaa
      781 ccgctgtttg cccttgtcga cagccactcg ttttacgtca ttggttattt tgaagaaacc
      841 aagttgcgcc atatccgcga aggtgcacca gcacaaatta ccctctatag tgataacaaa
      901 acgttacagg gtcacgtttc cagtatcggt cgggcgattt atgatcagag cgttgaaagt
      961 gactcaagcc ttattccgga tgtgaaacct aacgtcccct gggttcgtct cgcccaactc
     1021 gttcccgtcc gctttgcgct ggataaagtt ccaggcgatg tcacgctggt gtcgggcacc
     1081 acctgtagca ttgccgtagg tcaataatga acgcatcgtc atggtccttg cgcaatttgc
     1141 cctggttcag ggccacgctg gcgcaatggc gttatgcgtt acgcaatacc attgccatgt
     1201 gtctggcgct gacggttgcc tattatttga atctggatga accctattgg gcgatgacct
     1261 cggctgcagt ggttagcttt cccaccgttg gcggtgtcat cagcaaaagc ctcggacgca
     1321 tggctggcag tttactggga gccattgcgg cactgcttct tgccggacat acgctcaatg
     1381 agccgtggtt ttttctattg agcatgtcgg cgtggcttgg cttttgtacc taggcctgtg
     1441 cgcacttcac gaataacgtc gcgtatgcat ttcaactggc gggctacacg gctgccatca
     1501 tcgcctttcc gatggttaat attactgagg ccagccagct gtgggatatc gctcaggcgc
     1561 gcgtttgcga ggtgatagtc ggtattttgt gcggcggcat gatgatgatg atcctgccta
     1621 gcagttccga tgctactgcc cttttaactg cattgaaaaa catgcacgcc cgattactgg
     1681 aacatgccag tttactctgg cagcctgaaa caaacgatgc cattcgtgca gcacatgagg
     1741 gggtgattgg gcagatactg accatgaatt tgctgcgtat ccaggctttc tggagccact
     1801 atcgttttcg ccagcaaaac gcgcgcctta atgcgctact ccaccagcaa ttacgtatga
     1861 ccagtgtcat ctccagcctg cgacgtatgt tgctcaactg gccctcaccg ccaggtgcca
     1921 cgcgagaaat tctcgaacaa ttgctgacgg cgctcgccag ttcgcaaaca gatgcttaca
     1981 ccgtcgcacg tattatcgcc ccgctacgcc cgaccaacgt cgccgactac cggcacgtcg
     2041 ccttctggca gcgactacgt tatttttgcc gcctttatct gcaaagtagt caggaattac
     2101 atcgtctgca aagcggtgta gatgatcatg ccagactcgc acggacatcc gctctggctc
     2161 gtcataccga caacgccgaa gctatgtgga gcgggctgcg tacattttgt acgttgatga
     2221 tgattggcgc atggagtatt gcatcgcaat gggatgccgg tgccaatgca ttaacgctgg
     2281 cagcaattag ctgcgtactc tactccgccg tcgcagcacc gtttaagtcg ttgtcactgc
     2341 tgatgcgcac gctggtgtta ctttcgctat tcagctttgt ggtcaaattt ggcctgatgg
     2401 tccagattag cgatctgtgg caatttttac tgtttctctt tccactgctg gcgacaatgc
     2461 agcttcttaa attgcagatg cccaaatttg ccgcactgtg ggggcaactg attgttttta
     2521 tgggttcgtt tatcgctgtc actaatcccc cggtgtatga ttttgctgat tttcttaact
     2581 ataatctggc aaaaatcgtt ggcgttgcgc tggcttggct agcgttcgcc attctgcgtc
     2641 caggatcgga tgctcgtaaa agccgccgcc atattcgcgc gctgcgccgg gattttgtcg
     2701 atcagctaag ccgccatcca acactgagtg aaagcgaatt tgaatcgctc acttatcatc
     2761 acgtcagtca gttgagtaac agccaggatg cgctggctcg ccgttggtta ttacgctggg
     2821 gtgtagtgct gctgaactgt tctcatgttg tctggcaatt gcgcgactgg gaatcgcgtt
     2881 ccgatccgtt atcgcgagta cgggataact gtatttcact gttgcgggga gtgatgagtg
     2941 agcgtggcgt tcagcaaaaa tcactggcgg ccacacttga agaattacag cggatttgcg
     3001 acagccttgc ccgtcatcat caacctgccg cccgtgagct ggcggcaatt gtctggcggc
     3061 tgtactgctc gctttcgcaa cttgagcaag caccaccgca aggtacgctg gcctcgtaat
     3121 tacttaatta caccacaggc atagcgttca ccgccaccgc ccagcggttt aggttgatcg
     3181 gacatattat cgccgccaac gtggaccatc agcgccttgt ctttgatttc atccagtgat
     3241 ttcagacgag gcgcgatgac ggcatcggta gctttgccgt cattattgac gaccagtgca
     3301 ggcagatcgc ctaaatgccc ggtaccttct ggcccttcat gtttaccggt attttgtgga
     3361 tcaagatgcc cgcctgcgga ttccgcggcg ctggctttgc catctttggt ggctggctgg
     3421 cagcttcctt tggcatgaat atggaagcca tgttcaccgg ggggtaatgc tttcagatcg
     3481 ggtgaaaact ccagaccttt atcggtttca gtaatggtga cgctaccaat tgactgccca
     3541 accccttgcg acgtgacgag gttcatctcg actttttcac tggcagcttg tgcgccggtc
     3601 gcaacaacca gcgccagaat ggccagacta aaacgtttca taggacctcc gttcattgtg
     3661 cattcctgaa taagtgtaaa ccagtgacac atttttgaac ggcggctatt cctaaaagtg
     3721 cttacggtac gtcgtacccc agtgcagctt tacggatacg aaaccattgt tgacgggtca
     3781 ttttcagtgt ttctgcttcg acagctgccc gtacgcgctc aattttacct gagccgataa
     3841 ttggcagcgg ctgcgacggt aaacgtaata cccaggcgta aaccacctgt tcaatcgagc
     3901 ccgcgtttaa ctcctccgcc accacagcca gttcatcacg cagcggctgg aaataatcat
     3961 cattaaacag acgaccacca ccaaggcagg accacgccat cggacgaaca cgcagttgtt
     4021 gtagttggtc gagcgtgcca tccagcagta acggctgatg caccggggat atttccacct
     4081 gattagtggc aagggtaaac ggcagacgtg attgcaacag ggcaaattgc gcaggcgtaa
     4141 agttcgatac gccaaaatga cgcactttgc cgctctgatg cagatgtttg aacgcgtccg
     4201 ccacttcatc ggcatccatt aacgggtctg gtcggtggat taacagcaga tccagatgat
     4261 cggtcgcgag attaattagc gactgttcgg cgctcttaat gatgtgattg cggtcagtga
     4321 tgtaatgacc aatgacgttt tcttcacgcg cggtcgtcgc gataccgcat ttactgacga
     4381 tttccatccg ttcacgcagg tgaggtgcca gtttcagtgc ctcgccaaac gccgcttcgc
     4441 actgatagcc accataaata tcagcatggt ccacggtggt cacgccgaga tccagatgct
     4501 cttcaataaa actgaccagc tggcgggcgg acatattcca gtccatcaat cgccagtagc
     4561 ccatcacaaa acgggaaaac tccgggcctt ccggcgcaat agtaatacgc tgaaccataa
     4621 tcgcttcctc ttatcagata taagaggagt atacgcaaga ttaggttcaa aagagtgatg
     4681 gttgctccgg ttcgtctgat gacgctggct tatttgcgcg taatttgcgc attaatcgct
     4741 gccgacaaag gcgcagcacc tcttgttttt cgccatcgct cattttattc cagttaaaac
     4801 gctcatcccg actacgaaaa cagccgcgac aaaacccgcg ttcgtcagac tggcaaattc
     4861 cccggcacgg gctctggacg ggaaagaact ctaattgctc cgccacctcg ccctcctcag
     4921 ataagattat taccattatt gaagctgtta atgtccaaag tagcaacttt gcttgcacta
     4981 gaccaactgg tctactacac tccaacgcat gaacaaacac accgaacatg atactcgcga
     5041 acatctcttg gcgacgggcg agcaactttg cctgcaacgt ggattcaccg ggatggggct
     5101 aagcgaatta ctaaaaaccg ctgaagtgcc gaaagggtcc ttctatcact actttcgctc
     5161 taaagaagcg tttggcgttg ccatgcttga gcgtcattac gccgcatatc accagcgact
     5221 ggctgagttg ctgcaatccg gcgaaggtaa ctaccgcgac cgcatactgg cttattacca
     5281 gcaaacactg aaccagtttt gccaacatgg aaccatcagt ggttgcctga cagtaaaact
     5341 ctctgccgaa gtgtgcgatc tgtcagaaga tatgcgcagc gcgatggata aaggtgctcg
     5401 cggcgtgatc gccctgctct cgcaggctct ggaaaatggc cgtgagaacc attgtttaac
     5461 cttttgtggc gaaccgctac aacaggcaca agtgctttac gcactatggc ttggcgcgaa
     5521 tctgcaggcc aaaatttcgc gcagtttcga gccactggaa aacgcgctgg cccatgtaaa
     5581 aaacattatt gcgacgcctg ccgtttagca ggcatttttt atcaccagac gaccgggagc
     5641 ctttatgtca tctgaaaaac tgtattcccc actgaaagtg ggcgcgatca cggcggcaaa
     5701 ccgtattttt atggcaccgc tgacgcgtct gcgcagtatt gaaccgggtg acattcctac
     5761 cccgttgatg gcggaatact atcgccaacg tgccagtgcc ggtttgatta ttagtgaagc
     5821 cacgcaaatt tctgcccagg caaaaggata tgcaggtgcg cctggcatcc atagtccgga
     5881 gcaaattgcc gcatggaaaa aaatcaccgc tggcgttcat gctgaaaatg gtcatatggc
     5941 cttgcagctg tggcacaccg gacgcatttc tcacgccagc ctgcaacctg gcggtcaggc
     6001 accggtagcg ccttcagcac ttagcgcggg aacacgtact tctctgcgcg atgaaaatgg
     6061 tcaggcgatc cgcgttgaaa catccatgcc gcgtgcgctt gaactggaag agattccggg
     6121 tatcgtcaat gatttccgtc aggccattgc taacgcgcgt gaagccgctt ttgatctggt
     6181 agagctccac tctgctcacg gttatttgct gcatcagttc ctttcccctt cttcaaacca
     6241 tcgtaccgat cagtacggcg gcagcgtgga aaatcgcgca cgtttggtac tggaagtggt
     6301 cgatgccggg attgaagaat ggggtgccga tcgcattggc attcgcgttt cgccaatcgg
     6361 tactttccag aacacagata acggcccgaa tgaagaagcc gatgcactgt atctgattga
     6421 acaactgggt aaacgcggca ttgcttatct gcatatgtca gaaccagatt gggcgggggg
     6481 tgaaccgtat actgatgcgt tccgcgaaaa agtacgcgcc cgtttccacg gtccgattat
     6541 cggcgcaggt gcatacacag tagaaaaagc tgaaacgctg atcggcaaag ggttaattga
     6601 tgcggtggca tttggtcgtg actggattgc gaacccggat ctggtcgccc gcttgcagct
     6661 caaagctgag cttaacccac agcgtgccga aagtttctac ggtggcggcg cggaaggcta
     6721 taccgattac ccgacgttgt aatccaacat tgcgagcggc gtaaagccgc cgctatacta
     6781 aaacaacatt ttgaatctgt tagccatttt gaggataaaa agatgcgtct tcttcatacc
     6841 atgctgcgcg ttggcgattt gcaacgctcc atcgattttt ataccaaagt gctgggcatg
     6901 aaactgctgc gtaccagcga aaacccggaa tacaaatact cactggcgtt tgttggctac
     6961 ggcccggaaa ccgaagaagc ggtgattgaa ctgacctaca actggggcgt ggataaatac
     7021 gaactcggca ctgcttatgg tcacatcgcg cttagcgtag ataacgccgc tgaagcgtgc
     7081 gaaaaaatcc gtcaaaacgg gggtaacgtg acccgtgaag cgggtccggt aaaaggcggt
     7141 actacggtta tcgcgtttgt ggaagatccg gacggttaca aaattgagtt aatcgaagag
     7201 aaagacgccg gtcgcggtct aggcaactaa tctcctgccg ggcgtgaact catcgcgccc
     7261 gcatctttac tgcatcgaca agtaatattt gtcataatgc gcgctgcaat ttatccgtat
     7321 taagagaatc agatgtccga taacgctcaa cttaccggtc tgtgcgaccg ttttcgtggt
     7381 ttttatcctg ttgtgatcga tgttgaaaca gccggattta acgccaaaac cgatgcgctg
     7441 cttgagattg ccgccatcac cctgaaaatg gatgaacaag gctggctgat gccggacacc
     7501 acattacatt tccacgtcga accatttgtc ggcgcaaatt tgcaaccaga agccctcgcc
     7561 ttcaacggca ttgacccgaa cgatcccgat cgcggcgcgg tcagcgaata cgaggcgctg
     7621 cacgaaattt ttaaagttgt acgtaaaggt attaaagcga gcggctgtaa ccgcgccatt
     7681 atggtggcgc acaacgccaa ttttgatcac agctttatga tggccgccgc agaacgcgcc
     7741 tcgctgaaac gtaacccgtt ccaccctttc gccacttttg acactgctgc actggccggg
     7801 ctggcactcg gacaaaccgt attgtcaaag gcttgccaga ccgctggcat ggacttcgac
     7861 agcacccagg cgcactccgc gctgtacgac accgaacgca ctgctgtgct gttttgtgaa
     7921 atcgtcaacc gctggaaacg tctgggaggc tggccgctac ctgccgccga agaggtgtaa
     7981 tcgagtcgat gcctgatgac atgcaatgat tcaggcatct atagtgaggc tattccacgc
     8041 atcaagcatg atattcacgg ggaatagcgt taatggcaga taatccagac ccttcatcgc
     8101 tcctgccgga cgtgttttca ccggcgaccc gcgactggtt tcttcgcgcc tttaaacagc
     8161 cgaccgctgt ccagtcgcaa acctggcatg tggcggcgcg aggcgaacat gcgctggtga
     8221 ttgcaccgac tggctccggg aaaacgctgg cagcatttct ctacgccctc gatcggctct
     8281 tccgcgaagg cggcgaagat acccgcgagg cgcataagcg taaaacctca cgcatcctct
     8341 atatttcacc gataaaagcc ctgggcaccg acgttcagcg caacttgcag atcccgttga
     8401 agggtattgc cgatgaacgg cggcggcgcg gcgaaacggt aattattgat gaagtccacg
     8461 cggtagcggg cagtaaacgt ggtgcgcatc tggcgttaag tctggagcgg ctcgatgcgc
     8521 tgctccacac ctcagcacag cgaattggcc tttctgccac tgtgcgctca gccagcgatg
     8581 tggcagcatt tcttggtggc gatcgcccgg ttacggtagt caacccgccc acaatgcgcc
     8641 atccgcagat acgaattgtc gtaccggtcg ccaatatgga tgatgtctca tcggtcgcca
     8701 gcggcaccgg cgaagacagc catgccggcc gggaaggctc catctggcca tatattgaaa
     8761 cgggtatcct tgatgaagtg ttgcgccatc gctcgaccat tgtctttact aattcacgcg
     8821 ggctggcgga aaaactgacg gcacgattaa atgagcttta cgccgcacgc ttacagcgtt
     8881 ccccgtctat cgccgttgat gcggcccatt tcgagtcgac ctccggcgca acctctaacc
     8941 gtgtacaaag tagcgacgtt tttattgccc gctcacacca cggctccgtc tctaaagaac
     9001 aacgagcaat caccgaacag gcgctgaaat cgggtgaatt acgctgcgtg gtcgcaacct
     9061 ccagtcttga actggggatt gatatgggcg cggtggatct ggtgattcag gtggcaacgc
     9121 cgctttctgt tgccagtggg ttacaacgca ttggtcgcgc cggacatcag gttggcggtg
     9181 tatctaaagg gctgtttttc ccccgtaccc ggcgtgattt agtcgattcc gcagtcattg
     9241 tagagtgtat gttcgcaggc aggctggaaa acctgacacc accgcataat cctctcgacg
     9301 tccttgcgca gcaaaccgtt gccgccgcgg cgatggatgc attacaggta gacgaatggt
     9361 actcccgcgt acgccgtacc gcaccgtgga aagatctgcc aagacgtgtt tttgacgcca
     9421 cgctggatat gctttccggg cgctatccct ctggcgattt ttctgctttt cgccccaaac
     9481 tggtctggaa cagggagacc gggatattga ccgcccgacc tggcgctcaa ttgttggcgg
     9541 ttaccagcgg cggcaccatt ccggatcgtg gcatgtatag cgtgttatta cccgaaggtg
     9601 aagaaaaggc cggttcgcgg cgggtgggtg aactggatga ggagatggta tatgagtcgc
     9661 gggtgaacga cattatcact ctcggcgcta cctcatggcg gatccagcaa atcacccgcg
     9721 atcaggtgat tgtgactcct gctccgggtc gttctgccca gctccccttc tggcgtggtg
     9781 aaggtaacgg acgtccggct gaattaggcg agatgatcgg cgattttctt catttactgg
     9841 cggatggcgc gttcttttcc gggactattc ccccgtggct ggcagaagaa aatacgaacg
     9901 ccaatattca ggggttgatt gacgagcaac gcaacgcgac gggcatcgtt ccggggagtc
     9961 gccatctggt cctcgaacgg tgccgtgatg aaattggcga ctggcgtatt attttgcact
    10021 ctccctatgg aagacgggtg catgaaccct gggcgctggc gattgccggg cgaatacatg
    10081 ccctatgggg cgctgacgcg tctgtggtcg ccagtgatga cagcattgtt gcgcgcattc
    10141 ctgacactga tggtaaattg cccgacgccg cgattttttt gttttaacca gaaaagttgc
    10201 tgcaaattgt ccgcgaggcg gtaggcagct cggcactttt cgccgcccgt tttcgcgaat
    10261 gcgccgcgcg ggcattatta atgccgggcc gcacgccggg ccatcgcacc ccgctttggc
    10321 aacaacggct gcgcgccagt cagttgctgg aaatcgctca gggatatccg gattttccgg
    10381 tcattctcga aaccctacgc gaatgtctgc aagatgttta tgatcttccc gcactggaac
    10441 gtttgatgcg tcgcctgaac ggtggcgaaa ttcaaatatc cgatgtaacg accactacgc
    10501 cctcgccttt cgccacaagt ttattgttcg gctatgtcgc ggaatttatg taccagagcg
    10561 acgccccgct ggcagagcgc cgggcatccg tactgtcgct ggacagcgag ttactgcgca
    10621 atctactcgg acaggtcgat ccgggggaat tactcgaccc gcaggtcatt cgccaggtgg
    10681 aagaagagtt gcaacgactg gctcctggta gaagagcgaa aggtgaagaa ggattgttcg
    10741 acctgctgcg cgaactgggg ccaatgaccg ttgaagacct ggcgcaacgg catacaggca
    10801 gcagtgaaga ggttgcgtcg tatctggaaa atcttcttgc agtaaaacga atcttcccag
    10861 cgatgattag cggacaggag cgtcttgcct gtatggatga tgccgccagg ctgcgtgatg
    10921 ccctcggcgt acggctacca gagtcattgc cagagattta tttacataga gtcagttacc
    10981 cgcttcgcga cctctttctg cgctatctcc gggctcatgc tctggtcacg gctgaacaac
    11041 tggctcatga gtttagtctc ggtattgcca ttgtcgaaga gcagcttcag caactgcgtg
    11101 aacagggtct ggtgatgaat ctgcaacaag acatctgggt gagcgatgaa gtatttcgtc
    11161 gtctgcgttt gcgctcgctg caagccgcca gagaagcgac gcgtcccgtt gcagccacga
    11221 cctatgcgcg attgctgctg gaacgtcagg gcgtattacc cgccaccgat ggtagcccgg
    11281 cgctctttgc ctcaacatct ccaggcgttt atgagggcgt agatggagtg atgcgggtga
    11341 tcgaacagct tgccggagtc ggtttacccg cctcactctg ggaaagccag atcctgcctg
    11401 cccgcgtacg cgactattcg ccagaaatgc tcgatgaatt actggcaacc ggagcggtta
    11461 tctggtcggg acaaaaaaaa gctgggtgaa gatgacggcc tggtggcact gcatctacag
    11521 aaatatgccg cagaatcgtt cactcccgcc gaagcggatc aggcgaatcg ttcggcgctg
    11581 cagcaagcga taatcgctgt tctggctgac ggaggagcct ggtttgcaca acaaatcagc
    11641 caacggatac gcgataaaat cgtcgaatcg gttgattcct cggcactgca agaggcgtta
    11701 tgggcgctgg tctggcaagg cgtcattacc agcgatattt gggcaccgtt acgcgccctc
    11761 acccgcagca gttccaacgc acgcacctca actcgccgca gtcaccgggc tcgtcgtggg
    11821 cgtcctgtct atgcacaacc cgtctcgccg cgggtatctt acaacacacc aaatctggct
    11881 ggacgctggt cgttattgca ggtggagcca ctaaacgata ccgaaaggat gctggcgctg
    11941 gcggaaaata tgctcgaccg ctacggcatc atcagtcgtc aggcggtgat agccgaaaat
    12001 atccctggcg ggtttccatc gatgcaaaca ctttgtcgaa gtatggaaga ctccgggcga
    12061 attatgcgag gtcgttttgt agaaggtctg ggtggcgcgc aattcgctga acgtctgact
    12121 attgaccgat tgcgcgatct ggcgacacaa gccacgcaaa cacgccacta tacaccagtg
    12181 gcgctctccg ccaacgatcc ggctaatgtg tggggaaatc ttctgccctg ggctgcacat
    12241 ccggcaacgc tggtaccaac gcgtcgggcg ggtgcgctgg tggtcgtttc tggcggcaaa
    12301 ttgttactct atctggcgca aggcggcaaa aaaatgctgg tctggcagga aaaggaggaa
    12361 ttactcgccc cagagatttt ccaggcgctg accaccgcac tgcgtcgcga accaaggctg
    12421 cgctttacgc taacagaagt gaatgatctt ccggtccggc aaacgccgat gtttacgctg
    12481 ctgcgtgagg cgggattttc aagttcgcca caagggctgg attggggata gttgacatcc
    12541 tccacgccct gaaagacgtg gttccctgct acgttcaagc tgtcgcctga atcatttcgg
    12601 tgggttcctg cttcaacgag cggcctgact gcaccatctc tccacaggca agaacggcgt
    12661 gccccgccgc taaaatgtta cgagcgccat ttacatcggc gttcgctgta tatccacata
    12721 cctggcatct gaatttactt tgtgacaggc ggttttcttt cgctgtatga ccacagcacg
    12781 cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca ctccagagct
    12841 gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc gaacggttta
    12901 aacctgattt tgcccggaca ttgcgccccg gctggctgac cgtacccgct gctgactttg
    12961 acatgttgct gaccttcaaa tcctcaatga ctatcattgc gtggtttttg ctgacggtcg
    13021 ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acaggaatgc agtcgctgta
    13081 ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttccggctt aactgtcgct
    13141 gaagtctcgc cagcgtcttc tggttttttt gaaaactgtt tacaggctca aagactgtgc
    13201 catctgacag cgtggcgagt ttagccacgc cagcatccag cccgatcatt gatgctgaag
    13261 ggtgaaccgg agttgatact tcactttctg tctgaatact gatgtaccac gtaccgcagg
    13321 actggctgac agtgacattt ttcacaactc ccgtgacctg acggctattc cggtagcgca
    13381 tccagccaag tttcggcaga aaaatacggc tgttttcctg atcgagctta acgtcctgcg
    13441 ggtagcggaa tgcagcattc tgtccccgct ttttgaatcg gggaaaagca gcccgcttct
    13501 ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga ctgttgcaat ggctgtgagg
    13561 gcgaatcttt aagccattgc gtttcagtgg cgtttttcca ctcaaccagc caggaagcca
    13621 ttttcccgta agggatgtat ttattaccgg cctcatgatt ctcattctga agcgccagag
    13681 cacgattgaa aacgaaacga catgcgcctg cgaagcgcct catttcacgc tcctgttgac
    13741 caccgggtct taactggaat ttaaatgcct gtaatcgttt cacaggtcaa atgatacttt
    13801 ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca ttgtgttttc ctgatgcatg
    13861 ttcacctggt ctttgtcacc aaataccgac gccagatttt tgaccatgac acgacagaaa
    13921 aactacgcac ttacttttca aatgtatgtg ctgattttgc agctgaactg gttgaaatgg
    13981 atggcgaacc agatcacgtc catttgttaa ttaactatcc tcccaaactg gcgatatcca
    14041 gtctggtaaa cagcctcaaa ggcatatcga ataggttact gcgacgagat cgaccagata
    14101 ttgcagtcag gtattactac aaaggcgttt tgtggagtcc tggctatttt gccagtagct
    14161 gcgaaggtgc gccaatatcc gtcatccgcc agtacattga acaacagcaa acacctggtc
    14221 aggtggaaaa ccgcgcctta tatccccgcc ctgaaggacg aggttttacg gcgcaccgga
    14281 taaaggactg acggatgccc gttcgcatcc gtcagtattg caggacggat tattccgcgt
    14341 ccggctcttc agacttgtat ttagcggcag tttctttgat cagctgctgc agttcgccac
    14401 gctgatacat ttcgatcacg atatcacaac cgccgaccag ctcgccgtca acccacagtt
    14461 gcgggaaggt cggccagtta gcatatttcg gcagttccgc acgaatgtcc ggattctgca
    14521 gaatatcaac ataggcaaaa cgttcgccac atgcggcaag cgcctggact gcctgggcag
    14581 agaaaccgca gctcggcagt ttcggtgaac ctttcatgta cagcaggatc gggttttcag
    14641 caatctggcg ttggattttt tcgatagtgg tgctcattgt cttgcttcct caaacttctt
    14701 tacggcagta atctgacatt gtagcgggtc agtgcggcat cggaaaataa cattttcatc
    14761 acgcttttgc tattttatcc ctttgctcta tttgttgcat ttcaaatatt cgtttcttct
    14821 ctgatgcgcg gcaaactggc gtatttataa ccatttttag ctttcactgc tgcatttttt
    14881 tgcgctcgcc aacgaaacgt attttttaac aataaaagct attaactttc tcttcttcta
    14941 tgcattagaa tcatcaagtt ttgtaaatca gacgcaggca tgatagacct gccttaacag
    15001 agggacgctc agtggcgcgg ataaaccgta tttcgatcac gctctgtgct ttacttttta
    15061 ccaccctgcc tttaacgcct atggcccatg cttcaaagca agccagggag agttctgcta
    15121 ccactcatat caccaaaaaa gcagataaaa agaaaagcac ggcaaccacc aaaaagaccc
    15181 agaaagccag ccagaaaaca gcgaaaaaag ccgccagtaa aagtatgacc aaaagcaaaa
    15241 ccgcttcttc tgttaaaaaa tcttccatta ccgcttctaa aaacgccaaa actcgcagca
    15301 aacacgccgt caataaaacg gcctcagcca gctttaccga aaagtgtacc aaacgtaagg
    15361 gctataaatc gcattgtgtg aaagtcaaaa atgccgcgtc aggaaccctt gccgacgcgc
    15421 acaaagcgaa ggtgcaaaaa gccacgaaag tggcaatgaa taaactgatg cagcaaattg
    15481 gtaagccata tcgttggggc ggcagctcac cgcgtaccgg ttttgattgc agcggcctgg
    15541 tttattacgc ttataaagat ttggtgaaaa ttcgtattcc gcgtacggcg aatgaaatgt
    15601 atcacctgcg tgatgcagga cctatcgaac gtagtgagct gaaaaacggc gacctggtct
    15661 ttttccgtac tcagggacgc ggtacagccg atcatgtcgg cgtgtatgtc ggcaacggca
    15721 aatttattca gtcaccgcgc acaggtcagg aaattcaaat cacttctctc agtgaagact
    15781 actggcagcg ccactatgtt ggcgctcgtc gggtaatgac cccaaaaaca cttcgctaaa
    15841 actttaccct gttgttacgg caacagggta agttcatctt ttgtctcacc ttttaatttg
    15901 ctaccctatc catacgcaca ataaggctat tgtacgtatg caaattaata ataaaggaga
    15961 gtagcaatgt cattcgaatt acctgcacta ccatatgcta aagatgctct ggcaccgcac
    16021 atttctgcgg aaactatcga gtatcactac ggcaagcatc atcagactta tgtcactaac
    16081 ctgaacaacc tgattaaagg taccgcgttt gaaggtaaat cactggaaga gattattcgc
    16141 agctctgaag gtggcgtatt caacaacgca gctcaggtct ggaaccatac tttctactgg
    16201 aactgcctgg caccgaacgc cggtggcgaa ccgactggaa aagtcgctga agctatcgcc
    16261 gcatcttttg gcagctttgc cgatttcaaa gcgcagttta ctgatgcagc gatcaaaaac
    16321 tttggttctg gctggacctg gctggtgaaa aacagcgatg gcaaactggc tatcgtttca
    16381 acctctaacg cgggtactcc gctgaccacc gatgcgactc cgctgctgac cgttgatgtc
    16441 tgggaacacg cttattacat cgactatcgc aatgcacgtc ctggctatct ggagcacttc
    16501 tgggcgctgg tgaactggga attcgtagcg aaaaatctcg ctgcataata actgatggca
    16561 aatgcagcat tgcctgatgc gcttcgctta ccatgccaac gcggatgagg cgttaacagc
    16621 gcattcggca agataaatcg cacgttgtca gcaactgtaa cgcagaaggt tatccttctg
    16681 cgtttttgtt taattagctg ttagcaacgc aaactgtttc aggttgtttt ctggctgaca
    16741 taaacaccag caataatgcc agtcccgcga caatcgcccc cgtcaccggc acaaagctgt
    16801 atcccagccc agcggaaatt accgcaccac cagcagctgc tcccagcgca tttccaagat
    16861 taaaggcacc aatattgact gatgaagaca gacctggcgc ttcactggcg acatgcatca
    16921 cgcgcatctg taacggaggt acaaccgcaa aggttgctgc gccccacacc accatgctaa
    16981 tagctgcgcc gaactcattg cgggccagga acgggattgc cagcataatc accatcagca
    17041 gcaacaaaaa gcctttcaac gtgccgttaa ctgaacgatc tgccagtttg ccgccgagat
    17101 agttaccgat agagaatccg acaccaatca gcaccagcat tgccgtgacg aacaccggtg
    17161 ttgcgtgggt aatactttgc agtaccggag agatataggt gtagagggta aacattgcac
    17221 cagctcccag taccgtcgtc agcaatgcag acagcacctg cggacgcatt aataccgcca
    17281 gctctttttt cacttcaggt cgtgcccctg taccaccttt aggtaatgag aagaacagac
    17341 ttaccattga aatcactccc agccccgccg ttgccagaaa tgacatccgc cagccgatgg
    17401 tttcacccaa ccaggtcgcc gccggcacgc caccgatatt tgccagggtt aaccccataa
    17461 acatagtggc aactgcgctg gcctgtttat gttttggcac cacgcttgcg gccacgactg
    17521 aacccaaacc aaaaaatgct ccgtgattca ggctggtcaa aatacgtgaa agcatcaggg
    17581 tcatataatc cggcgcgatg gcggaaagaa cgttgcctag cgtgaaaatt gccatcagga
    17641 aaatcaacgc actgcggcgg gcacgatgag aaagtagaag cgtcatcagc ggcgcgccca
    17701 ccattacgcc aactgcatag gcactgatta acattccggc agcgggaatc gagacatcca
    17761 caccgcgcgc aatgacgggc aacaagccca ttggcgagaa ctccgttgtc ccgataccaa
    17821 acgcgccaat cgccagcgcc agcaacggat agttaatttt catgccttat ctccacctct
    17881 tcgcgtcatt acgcgatatt cattaaagtg gcgaaagcat gacagcaatc acaaaaaaat
    17941 gaaaataaca aaaagagaaa acacttttgc cattttgcta acaaacagga aggagatgcg
    18001 agggagaacg cgctccctcg agaggaaatc agtgcagcgc ggcagtcaaa cccacggcta
    18061 cgatcaaacc gaggacgata accgttgtta ccagtgaaaa tttaaggtcg gtgctcatca
    18121 agttttctcc ttttttatta ccacacaaaa agtgatatta cgcattttta cacactgtga
    18181 tgaaaaaatc tcccgtcatt tataatgata agtgttttta ccacttcccc ttttcgtcaa
    18241 gatcggccaa aattccacgc ttacactatt tgcgtactgg ccattgaccc cttcctgacg
    18301 ctccgtgtcg tttttccggc gtaccgcaac acttttgttg tgcgtaaggt gtgtaaaggc
    18361 aaacgtttac cttgcgattt tgcaggagct gaagttaggg tctggagtga aatggaatgg
    18421 caacaataaa agatgtagcg aaacgagcaa acgtttccac tacaactgtg tcacacgtga
    18481 tcaacaaaac acgtttcgtc gctgaagaaa cgcgcaacgc cgtgtgggca gcgattaaag
    18541 aattacacta ctcccctagc gcggtggcgc gtagcctgaa ggttaaccac accaagtcta
    18601 tcggtttgct ggcgaccagc agcgaagcgg cctattttgc cgagatcatt gaagcagttg
    18661 aaaaaaattg cttccagaaa ggttacaccc tgattctggg taatgcgtgg aacaatcttg
    18721 agaaacagcg ggcttatctg tcgatgatgg cgcaaaaacg cgtcgatggt ctgctggtga
    18781 tgtgttctga gtacccagag ccgttgctgg cgatgctgga agagtatcgc catatcccaa
    18841 tggtggtgat ggactggggt gaagcaaaag ctgacttcac cgatgcggtc attgataacg
    18901 cgttcgaagg cggctacatg gccgggcgtt atctgattga acgcggtcac cgcgaaatcg
    18961 gcgtcatccc cggcccgctg gaacgtaaca ccggcgcagg ccgccttgcc ggttttatga
    19021 aggcgatgga agaagcgatg atcaaggtgc cggaaagctg gattgtgcag ggtgactttg
    19081 aacctgaatc cggttatcgc gccatgcagc aaatcctgtc gcagccgcat cgccctactg
    19141 ccgtcttctg tggtggcgat atcatggcaa tgggcgcact ttgtgctgct gatgaaatgg
    19201 gcctgcgcgt cccgcaggat gtttcgctga tcggttatga taacgtgcgc aacgcgcgct
    19261 attttacgcc ggcgctgacc acgatccatc agccaaaaga ttcgctgggt gaaacagcgt
    19321 tcaacatgct gttggatcgt atcgtcaaca aacgtgaaga accgcagtcc attgaagtgc
    19381 atccgcgttt gattgaacgc cgctccgtgg ctgacggccc gttccgcgac tatcgtcgtt
    19441 aatcacccgt tgcgggagtc tcttccggct cccgcagcca ctccttattc agcgtctcac
    19501 tatcgctgag atactcaagc agccaggtta acgcaggcga catatcattt tgctgccatg
    19561 tcagacaaca tgccgaatcc gggaaggggt tttccagttc aagtgctacc cacttcccct
    19621 cattaagcca cggtttggcg aaatgtgttg gcaccatccc tatgcataat cctgccgaga
    19681 tacaggttgc cgatgattcc cagtcaggta cgacaagtct tttttggtta tccagcaacc
    19741 aggtaatacg tttaggtagc gttcgcgagg tgtcttcgcg caccaacgac ggccagttgc
    19801 gcaacgtatc atcgctgaac gggccatcca tcaacgccag cgggtggtga ctggcaacaa
    19861 cgcaactcca gcttagcatc cccatatccc ggaaggcata acgaccgcct accggaatcg
    19921 cgcgtgttgc gccaattgcc agttccacgc gcccgtcgga aagcgcatcc cagacaccgt
    19981 tgaacacttc ctgaaagaca agaagttcga catcatcaaa atggcgataa aaatcaacga
    20041 tcatctgccg tgtacgttct ggcctgacaa tattatccac tgcgatagct aactgaccgc
    20101 gccagccgtt cgctatctgc tgacattgct ggcgggtgat ctgcattttt ttgacaacag
    20161 agcgcccttc tttgagaaac cacgctccag cagcggtcag ctccacatca cggtgccgtc
    20221 gttcaaagag cggtaccgcc agccactctt ccagctgacg cacggtatag ctgaccgcag
    20281 aaggaacacg atgcagctcc tgtgccgcag cgctaaaact accattacgc gctaccgcat
    20341 caacaacttc gagtgaatat tctgaccaca tagtctgcct gcaaaatttt tgaaaccagt
    20401 catcaaatat taccgtttca caacactaat ttcactccct acactttgcg gcggtgttta
    20461 attgagagat ttagagaata tacatgcaac ctgggaaaag atttttagtc tggctggcgg
    20521 gtttgagcgt actcggtttt ctggcaaccg atatgtatct gcctgctttc gccgccatac
    20581 aggccgacct gcaaacgcct gcgtctgctg tcagtgccag ccttagtctg ttccttgccg
    20641 gttttgccgc agcccagctt ctgtgggggc cactctccga ccgttatggt cgtaaaccgg
    20701 tgttattaat cggcctgaca atttttgcgt taggtagtct ggggatgctg tgggtagaaa
    20761 acgccgccac gctgctggta ttgcgttttg tacaggctgt gggggtctgc gccgcggcgg
    20821 ttatctggca agcattagtg acagattatt atccttcaca gaaagtcaac cgtatttttg
    20881 cgaccatcat gccgctggtg ggtctgtctc cggcactggc tcccctgtta ggaagctggc
    20941 tgctggtcca tttttcctgg caggcgattt tcgccaccct gtttgccatt accgtggtgc
    21001 tgattctgcc tattttctgg ctcaaaccca cgacgaaggc ccgtaacaat agtcaggatg
    21061 gtctgacctt taccgacctg ctacgttcta aaacctatcg cggcaacgtg ctgatatacg
    21121 cagcctgttc agccagtttt tttgcatggc tgaccggttc accgttcatc cttagtgaaa
    21181 tgggctacag cccggcagtt attggtttaa gttatgtccc gcaaactatc gcgtttctga
    21241 ttggtggtta tggctgtcgc gccgcactgc agaaatggca aggcaagcag ttattaccgt
    21301 ggttgctggt gctgtttgct gtcagcgtca ttgcgacctg ggctgcgggc ttcattagcc
    21361 atgtgtcgct ggtcgaaatc ctgatcccat tctgtgtgat ggcgattgcc aatggcgcga
    21421 tctaccctat tgttgtcgcc caggcgctgc gtcccttccc acatgcaacc ggtcgcgccg
    21481 cagcgttgca gaacactctg caactgggtc tgtgcttcct cgcaagtctg gtagtttcct
    21541 ggcttattag tatcagcacg ccattgctca ccaccaccag cgtgatgtta tcaacagtag
    21601 tgctggtcgc gctgggttac atgatgcaac gttgtgaaga agttgactgc ccgaatcatg
    21661 gcaatgccga agtcgctcat agcgaatcac actgatctat atcgatatac ttatacttag
    21721 gctgctaaca aaattttgtt gtatgattga aattagcggc ctatactaat ttcgagttgt
    21781 taaagctacg ataaatatta tgtttttacg gggacaggat cgttcccgac tcactatgga
    21841 tggtcatttc ggcaagggtt cctcctttcc ctctgttcta cgtcggatta tagactcgcg
    21901 gttttttctg cgagatttct cacaaagccc aaaaagcgtc tacgctgttt taaggttctg
    21961 atcaccgacc agtgatggag aaactatgag ttcatcgtgt atagaagaag tcagtgtacc
    22021 ggatgacaac tggtaccgta tcgccaacga attacttagc cgtgccggta tagccattaa
    22081 cggttctgcc ccggcggata ttcgtgtgaa aaaccccgat ttttttaaac gcgttctgca
    22141 ggaaggctct ttggggttag gcgaaagtta tatggatggc tggtgggaat gtgaccgact
    22201 ggatatgttt tttagcaaag tcttacgcgc aggtctcgag aaccaactcc cccatcattt
    22261 caaagacacg ctgcgtattg ccggcgctcg tctcttcaat ctgcagagta aaaaacgtgc
    22321 ctggatagtc ggcaaggagc attacgattt gggtaatgac ttgttcagcc gcatgcttga
    22381 tcccttcatg caatattcct gcgcttactg gaaagatgcc gataatctgg aatctgccca
    22441 gcaggcgaag ctcaaaatga tttgtgaaaa attgcagtta aaaccaggga tgcgcgtact
    22501 ggatattggc tgcggctggg gcggactagc gcactacatg gcatctaact atgacgtaag
    22561 cgtggtcggc gtcaccattt ctgccgaaca gcaaaaaatg gctcaggaac gctgtaaagg
    22621 cctggatgtc accattttgc tgcaagatta tcgtgacctg aacgaccagt ttgatcgtat
    22681 tgtttctgtg gggatgttcg agcacgtcgg accgaaaaat tacgatacct attttgcggt
    22741 ggtggatcgt aatttgaaac cggaaggtat attcctgctc catactatcg gttcgaaaaa
    22801 aaccgatctg aatgttgatc cctggattaa taaatatatt tttccgaacg gttgcctgcc
    22861 ctctgtacgc cagattgctc aatccagcga accccacttt gtgatggaag actggcataa
    22921 cttcggtgct gattacgata ctacgttgat ggcgtggtat gaacgattcc tcgccgcatg
    22981 gccagaaatt gcggataact acagtgaacg ctttaaacgg atgtttacct attatctgaa
    23041 tgcctgcgca ggtgctttcc gcgcccgtga tattcagctc tggcaggtgg tgttctcacg
    23101 cggtgttgaa aacggccttc gagtcgctcg ctaaaggcta ttctatcgcc ccctcttcgg
    23161 gggcgagttc agatcaggct tctgtacctg gttgattcat ggcattttct cgtgccgcca
    23221 gcacacgttc taccgtatct accactgcct gagtttgtgg atcgatttca atgttgacgc
    23281 gtgcgccaag ttttttcttc ccaagagtcg tgcgttccag tgtttccgga attaaatgga
    23341 cgcaaaaacg cgttggcgtg acttcgccga cggtcaggct aataccgtcg atgccaataa
    23401 atcctttgta caggatatat ttcatcaact gactatcctg gactttaaac cagatctggc
    23461 gattattttc cgaggttaat attttcgcca cttcagcggt ggtcataata tgacctgaca
    23521 ttaagtgtcc gccaatttca tcactgaatt tcgccgcacg ctcaacgttt acccaatccc
    23581 ccacttttaa atcgccaaga ttggtaatgc gtaacgtttc tttcatcagg tcaaaactga
    23641 catggttgcc gttaatttcc gtcacggtca ggcagcaacc gttatgcgcc acggaagcac
    23701 cggtttccag gccgtccagc atgtggtcgg gtaactccac cacatgcgta cgaaaatttg
    23761 gtttctcgtc aatcgacacc agttttgcgg tgccctgtac aatccccgta aacatactta
    23821 caactcctga aatcagttaa gacattctgt tcagcacaat agcaggtgga aaacgccctt
    23881 accagtgaag gggtaagaat ggctattttt tcactggaga attaataaat cctcgctaca
    23941 atagactgaa tttcccctgc ttcttctttt tgctgcccat tcaggcggct ttttagtctc
    24001 tcatataact acaaataaaa ggtgttcacg tgcagaagta tatcagtgaa gcgcgtctgt
    24061 tattagcatt agcaatcccg gtgattctcg cgcaaatcgc ccaaactgcg atgggttttg
    24121 tcgataccgt gatggcgggc ggctatagtg ccaccgacat ggcggcggtc gctatcggta
    24181 cttctatctg gcttccggcg atcctctttg gtcacggact gctgctggca ttaacgccgg
    24241 ttatcgcgca attaaatggt tccggtcgac gtgagcgcat tgcgcatcag gtgcgacaag
    24301 gtttctggct ggcaggtttt gtttccgttc tcattatgct ggtgctgtgg aatgcaggtt
    24361 acattatccg ctacatggaa aacatcgatc cggctctggc ggacaaagcc gtgggttatc
    24421 tgcgtgcgtt gttgtggggc gcgccgggat atctgttctt ccaggttgcc cgtaaccaat
    24481 gtgaaggtct ggcaaaaacc aagccgggta tggtaatggg ctttatcggc ctgctggtga
    24541 acatcccggt gaactatatc tttatttatg gtcatttcgg tatgcctgag ctcggtggcg
    24601 ttggttgtgg cgtggctact gcggcggtgt attgggtcat gttccttgcc atggtttctt
    24661 acattaaacg cgcccgctcc atgcgcgata ttcgtaacga aaaaggcacc gctaagcccg
    24721 agcctgcggt tatgaaacga ctgattcaac tcggtttgcc gattgcgctg gcactgttct
    24781 ttgaagtgac actgtttgcc gtcgtggctc tgttagtgtc tccgctcggt attgttgatg
    24841 tcgcaggaca ccagattgcc ctgaacttta gttcactgat gtttgtgctt ccaatgtcgc
    24901 tggcggcagc ggtaactatc cgcgtaggtt atcgtctggg tcagggctca acgctggatg
    24961 cgcaaaccgc tgcgcggacc gggcttatgg tgggtgtctg tatggcaacc ctgacggcca
    25021 ttttcacggt ttcactgcgg gagcaaatcg ccctgttgta caacgacaat cccgaggttg
    25081 taacgctggc tgcgcatttg atgttgctgg cggcggtata tcagatttct gactcaatcc
    25141 aggtgattgg cagtgggatt ttgcgtggtt ataaagatac gcgttccatt ttctatatca
    25201 cctttacagc ttactgggtg ctgggcttgc caagcggcta tattctggca ctgaccgatc
    25261 tggtcgttga acctatgggg ccagcaggct tctggatagg ctttattatc ggcctgacat
    25321 cggcagccat tatgatgatg ctgcgtatgc ggttcctgca acgtatgccg tcagccatca
    25381 ttctgcaacg agcgtcccgc taataaagac aaggcgcaac cttcacgggt tgcgcctgtg
    25441 tttttacgca ggctggagcg ttgcgccaat cccgtcttcg tctggctgta atttcagagc
    25501 gttacacaga gtttgccgac tggcatctgt tatctcaacg cgtatatccc cgttaacttc
    25561 ccctttcaca ttgatgaagt caggtgaaat ttctttcgca ctggtttgca ggcaaatcgc
    25621 tccctctccg cgcaggcgtt tcactttcag ttgcacatgc gttgccgcac cagtgatcag
    25681 ccacagtttt ccgccattca gattgatgtt accaacaatg aaagtgacgc tgtcaccttt
    25741 taccgtactg ccgtcgagaa tcaacacacc gccctgctgt acagtaatcg ttgattgttg
    25801 cagctctgcg ccataaccaa taatcattga agcgccgtcc agtaatgcaa cactcccggt
    25861 cagttcaacc ggtgcgcgat cttcctctac gacaaaattt tcgtccgcgc tggtgggtga
    25921 caacaggatc actgttccac cttgtattac cgcattttcc agcgtaccgg catgcacttc
    25981 agcccgcccc cccacattca ccgacaggtt ttgcgtctta ccggaactgt aaagctgaag
    26041 gccatccgtc cccagacgat aaatggatcc attctcaaca atagtgtcag agtcttcacc
    26101 accgagatta ttcatggtgc caccagcaag caacgtatca ctggctttcc cggccagcat
    26161 aaaaacgccg ccctgattaa tctcacaatt gctggcttct ccacttggat acaccaacaa
    26221 ttcaccacct tcatctacca cgaccgctga ggttgtccca ttaaccaaca ggccgccctc
    26281 ttgatcgaga atgatttttt ccgcgcgatg tccttccagt acacgcaggt taccgccatt
    26341 ttccagcaac aaaccgcagg cataaccctg atcaacgctg aattcacctt cgggatggcg
    26401 gccattaacc gtagcgagcg tagagagact aattgcgcct ccctcggact gaacaatatc
    26461 tgtggcaatg gcaccgtcgc tgaccgatag cgtgccattg ttaccgtact gtgtgcattt
    26521 ggcgatccca ccggttgcca ctttcaacca gccagccccc gtgaccacgg tattttcggc
    26581 tcttccgcca gcttcaacaa tcaactcgcc ttcagtaagt tgcgtcgcgc caaggacctc
    26641 gccggtctta acaatttgca cattcccgtc gctcatcaag tttttcgcat cagatcccat
    26701 gatttattcc tttgctgcat ctgtgtgcct ttattgctac ctaagtgtaa aggctacgga
    26761 ggatttatcc acgacagatt tgagatggtg gcaaacaact ctgtttaaac tctgatacac
    26821 gaattattgg gttgtatcag atgtaaatgc gatcctgaat aaaaatcgcc cttgcaaatc
    26881 aacaaaatat agccaatttg cttaaaagct ctgcaaacgg tcgaaatagc gcagaaaatt
    26941 acgttttgcc tcttgccacc tttccactct gccgctaata ttcgtccccg ttgtcaccta
    27001 caacgttgcg ttcatagctc agttggttag agcaccacct tgacatggtg ggggtcgttg
    27061 gttcgagtcc aattgaacgc accatcctgc gtccgtagct cagttggtta gagcaccacc
    27121 ttgacatggt gggggtcggt ggttcgagtc cactcggacg caccagattt tcttaatctg
    27181 gtcttttcct ttttccctct gtttcttctc tgtatccaat acgttaaaag atttacactg
    27241 tcttcgtatg cgttatcaga aggagaatcg ctatggcaac tttgttacaa cttcattttg
    27301 cttttaatgg cccatttggc gacgcaatgg ctgagcagct taaaccactt gctgagtcga
    27361 ttaatcagga acctggtttt ctgtggaagg tatggacaga aagtgaaaag aaccacgaag
    27421 ccggtgggat ctaccttttc actgatgaaa aaagcgccct tgcctatctg gaaaaacata
    27481 ctgcccgact gaaaaatctc ggcgttgagg aagttgtcgc caaagttttc gatgtcaatg
    27541 agccacttag tcaaatcaat caggcaaaac tcgcctgaca gaatttaatc aagggcggtt
    27601 agcgcccttt tcatccctgt ctgaaatttc tcaaattcta aaaatctcaa ccaaacttat
    27661 ctgataacac taaattcgaa agaatgcgta caggtaagta acaatgaaaa aaattgctat
    27721 tgtgggtgcc gggcctacgg ggatctacac cttattctcg cttctacagc aacaaactcc
    27781 actttctatt tctatcttcg agcaggctga cgaggccggt gtcgggatgc catacagtga
    27841 tgaggaaaac tcaaaaatga tgctggcaaa tattgccagt attgaaatac cgccgattaa
    27901 ttgtacgtat ctcgaatggc tacaaaagca agaagccagc catctccagc gttatggcgt
    27961 taaaaaagaa accttgcacg atcgtcagtt tttaccgcga attctgctgg gcgaatattt
    28021 ccgcgatcaa tttttacgat tagtagacca ggcacgacag caaaaatttg cagtggctgt
    28081 ttatgaatca tgccaggtta ccgatctgca aattacaaat gctggcgtca tgctcgctac
    28141 aaatcaggat ttacccagcg agacgtttga tttagcggtg attgccacgg gtcacgtctg
    28201 gcctgatgaa gaagaagcaa ccagaacgta ttttcccagc ccgtggtcag gcctgatgga
    28261 agcaaaggtc gatgcgtgta acgtgggtat tatgggaaca tccttgagcg gactggatgc
    28321 ggcaatggca gtggctattc agcatggttc gttcattgaa gatgataaac aacacgtcgt
    28381 ttttcaccgc gataacgcaa gtgaaaagct aaatattacg ttgatgtcgc gcacgggtat
    28441 tttacccgaa gccgatttct attgccctat tccctacgag cccttacaca tcgtcaccga
    28501 tcaggcatta aatgctgaga ttcaaaaagg tgaagagggc cttttggatc gggtatttag
    28561 attgatagta gaggaaatca agtttgctga tccagactgg agccaacgca tagccttaga
    28621 gagcctgaat gtcgattcct ttgctcaagc ctggtttgcc gagcgcaaac aacgcgaccc
    28681 atttgactgg gcagaaaaaa atctccagga agtcgaacgc aataaacgag aaaaacatac
    28741 tgttccctgg cgttatgtca ttctgcgcct gcatgaagcc gtacaggaaa ttgttcctca
    28801 tctgaatgaa cacgaccata aacggttcag taaaggcctt gccagggttt tcatcgataa
    28861 ttatgcggca atcccttcag agtctattcg tcgactactg gccttacgtg aagcggggat
    28921 ctgaaatccc tgatgttggc gaagattata cgttacagca acccgaagat attcgtggac
    28981 gcgtagcgtt cggcgcgttg ccctggttga tgcacgacca gcctttcgtt cagggactta
    29041 cggcatgtgc agaaattggt gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg
    29101 ctcgtcggcg tctttcgttt gattaaaggt aaagctatca gcactcaaca aactgatgct
    29161 gttcattaag acgatagcgg actccggttc gaatattgtt ttcaccttca atggcgacgg
    29221 cgaagcgaac tctttcgccg tcatgatatg ccagtgcggc gcttccccct agcccgagaa
    29281 taattgagtc gaccacgccc gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt
    29341 gtacattgtc gccgctgttg gcaattctgg cgtttgcgcc aaagctggcg atttgcacca
    29401 ggtcaccatt actggcgaca tgacaccgtt cacccaatgt acaaacgcgt actcgcattc
    29461 cggtattcgc aatccgacta ctatctccag cgctgcttat ccgcgaagaa ttaccggtta
    29521 cagcaacccg tgcgcgttca ccggagctgc caatgtgact gttaaagccg acacttccaa
    29581 tccgcacgct ataacccata ctggcaattt gcgcggcata cccagcggtt gcgattcggg
    29641 cgttatcctc acaacagccg atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa
    29701 atggtatctc tccggggtct gtggccagtt tttgcataga ggaaacttcc tgatgcgcga
    29761 agttttcatc cgccagccat tttttccacg catactcaac cagactctcc gcccagtccg
    29821 tatatccttc ctcaatcagt gcatgacgaa tatcggcata actaccgccg cgtgggaaat
    29881 ggcgcaggaa ccagcgatac atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg
    29941 taatgatcat tcgttagtgc cggtgatagc catcgaccat gcttttaaag gtttcgtgtg
    30001 gcgtacgccc cgtggtgcaa agataaagat gaccgaagat aaaaaagagg cttataaatg
    30061 ccagagcaaa atgcgcctgc aataaccagt atcttacgcc aggaaacaca tctcccacgg
    30121 cttgcggata gagacacaac agccccgtca atagtaacaa cggcagcaat ccatacatga
    30181 caccaacata ggcgacctgc tgtaagggat taaatttaga ctgtgttgtt gccgggaaag
    30241 gatgttcttc cccctgcata atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt
    30301 ccagccaccc ctgacgacga atgcgatagt ggtgaccatt atccccaacg gcattgatca
    30361 gcacaaagcc gagccagcat gccagtaaca aaaatccgca aacttcatgc accgcaacca
    30421 gacttttaac cgcagttgcg cccaccatcg caaagtgatt tatcagccca cttgccagca
    30481 acaatacaaa gagtaacgca ttcgaccagt gccataatct gaccgctttg ctgtaaagat
    30541 aaaccttctc accatgatcg gtggctgatt tcttcacgcc acgagcacga agcaaggcgt
    30601 gcagccccag caccaaccac atcccaacca gcaatactcc ggcaatgatc aaccacaccg
    30661 gccaaaattc gggagtgaac tgtggcacat agttcgctaa ctggctctga aactgttcag
    30721 catgttgcga cgggttcata cattttcctt tttaatcaaa tgttgaccga accggcgata
    30781 cagatgcggt tttccggcac caggtagctg atattgatag tatttattat cctgcaacca
    30841 cgcctgaatt tctgggctat cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct
    30901 gacgcaaatg ggcggaaagc cttttgccag tcgggactca gcgcagaaat cacatttatc
    30961 tgccacttta gtgacagggt taaggtagcg aaccaggtac ggacaagcgc caatgcaata
    31021 actgcagccg atgcactgcg atttttctac ccgcacgatc ccctgctcat cacgccacga
    31081 tgcccccgtc gggcatacgt cgatgcacgg agcatcttcg cagtgctgac atgactggcg
    31141 aaaaaagtga tactgcgttt cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa
    31201 acggcttccc tgagcgggga cgtggttagt tttacggcat gcgcgggcgc aaatattaca
    31261 gccattgcac cgtgactcgt catgaatcat ggcataccga acttcctttt cttgcctcgt
    31321 gttcgctaac agagaagacg cagaaccagt aaaaaagatt actgttccca tccccagaac
    31381 aaatttgcgt cgagtgaagg acatctgatt atcccctaat gcagtggctg caactgtcca
    31441 ccctatccat gacatctccc tgaaatttca ggggttctga aactgctcta cctcgaacca
    31501 ttcgcataag ccataagcct tgccataggt cataaatcgc tcgctaaaca agtacaacac
    31561 cgccgtcgat gactccacct gccgtatatc ggcatattca ggtgcaacat ccatcgcggc
    31621 gatcgcggct tcaatctgcg cttcctggaa gtaatatggc gcttgcatca gcatggcgac
    31681 tttgtaagga cgaggatacg tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca
    31741 aatgtcctgc tccacaactt gcaaagacat cgccgcgtag ttttcactca tggcctgcgt
    31801 tgagtaatag taatcatcct gacttcccgt catacaagcg atatccgcgt agtctccgtt
    31861 ctgcttaatg ctttccagca aaactgacag ttcctcttct gcaaccgaat agggtggcga
    31921 gagaaacacg gtgcgcgcca ccagctgccc agccgcagaa tgctggcgaa tgaactcagc
    31981 caggatttca gcaggttctg gttcggttac cgtatcaacg gggtgacgca tcccctgcaa
    32041 ccattgccgc ttcgcctcgt caacttcgct gactttcgct aacggcaatt catcctgatg
    32101 gttcatctta cccattcctt acgcaggcag tagattgtgt gctgccagat cggcggcaat
    32161 atcttccagc cccagacgct gcaatgtttc gcgggttgga caaccaagct gtgggtccca
    32221 gcccatttct ttgtagaaca tcgtgagcga agcatgcata tcgtcacgat ccattttgtc
    32281 agtaccttca gtaaagaccg ggatctgcgg atccttgtcg aaaacccagg aacagataag
    32341 atcgtgttca ttacgcatat ctttggtttg catcggtttt accgtgtagg cacgatgcaa
    32401 cgtaaagata cgctctgcag ctaaatctaa tttttcctga gtcatctctt cgccggtgat
    32461 cgctttgaag aatttggctt caagcgccag atcgccccgg taattacggc ttttcagcgg
    32521 cgaaacggtc attggccaga cccagttgca cagagtgacg gcgttatgca aacagacccg
    32581 caacagcgac cactttgcat atttaatttt tgcgtcattg attggcgtgt agtttttggt
    32641 ttcatcgtaa gcatcttcag aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa
    32701 tggcaagccg gaaccaataa agttgatatg ggtatgcgtc atgcagtcac ggttgaacat
    32761 acacttaaca atggaaccga cctgcgctga cgcttcattg gcatggtgaa ccggatagcc
    32821 aaacggcgac cagagtttat ttttcgcgta gccccagtac tctttaccca aattccagcg
    32881 ttctgcgatg gcatatgaac catcagccag gtgactcagc tcacccacac gatgcgccag
    32941 acggtagtaa aaatctttaa tgaagttaac gtcacccgct tccagttgat cccagcgaat
    33001 ttctgcatac tcttcagctg gcagaacacg cttgaacaca cctttgctgt aacaataagt
    33061 aaagtcgcga tgcaactgcc cgtagttaca ccataggccg tagtcatcga acaaattcag
    33121 accaaccagg ttaccaatca cacgaccatc gtctttatct tcaaaatctt tcggcccgtt
    33181 cgggaagatg gtggtatgga caaagtttgc tacacaagtg ttaccacctg tgctgggcac
    33241 gccaaactct ttcacccgag gaatattcat ttgggtcata caacggatcg ggcaagagtg
    33301 gcagccgctc atttttactg tgtatttctc tgccgccggt cctaagtcaa aaacggattt
    33361 ataggtacga aagccgaccg tattctgatt gcctggcgga atttcacccg tttcaatcgg
    33421 accaccttca gccgcgcccc aaaacagccc tttacgtgct gtccagcgtg acttgggatc
    33481 tgaatactct gcccacgatt gtggcgtact tggcacgaca tggttgttat tcgcaccaat
    33541 aagttcagtc atcatgtaat cattgagacg cttcatctcc tgacgatcgg caatgttgac
    33601 ccctttcgtc ccttcaaccg caatcgcttt caggtttttc gaacccatta ttgcgccagt
    33661 tcccgcaccg ccgctgtggt tacggctatt caacatgcca gagagaggaa taaggttttc
    33721 cccagcctga ccaatagccg ccacacaggt ttccgggctg gtcaatcgac aaatttcttc
    33781 cgtcgttgcg cgcgtccctt ttccccataa gaaatcggct ttttccaggc taactttgtc
    33841 atctttaatc ttcagccata ccggtgattt cgccttccct tcgataataa tgacgtcgta
    33901 tccagcgaat ttcatttgcg ctgcaaaaaa gccacccata tgggcatcgc gactaaattt
    33961 cctttggtaa aagtagaaag tgaggtgata tttacgcgag aactacaggg ggcaccagat
    34021 ccaggtaatg ggccggtagc aaagactaat ttattcgctt catcgaaagg tttcgtgcct
    34081 agcggtactt cgtcatacat aattttgtag ccgaagccca tgccaccgac aaaactttta
    34141 aacttactgg aatcttcgag ggtaatattt cctgtcgtga gattgactct taatatatta
    34201 cctgtccaac cgttagccat gattttttcc tttgcaagat tcacacagta atatctttcc
    34261 actcgataat ttttaacgcc cctgttgggc aggcgtttgc gcattcaccg cataacacac
    34321 atttcgagga ttttttactt tcggtattta cggtggccat catccacggg catgccgtag
    34381 tacaggcgct acagccaata caacgtttat gatcgacggt aatacagcct tctttctgct
    34441 gccaggtaat cgcaccaatc gggcagacgt tcatgcattg cggctctttg cattgacggc
    34501 aggtgtccgc ggtatagttg agatcgccat acaggccgcc gccagagcca accccgttgt
    34561 cgccaaagaa ataattgcga tggattttga tacgggagaa gaatgtccct actgagccat
    34621 cgttgaagtt ggtacaagag atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg
    34681 tcaccagcac ccctttgggt gtatttatca accctactgt gccgctatca atatcttcct
    34741 gtttgcaacc caaaagtgac agtaacgcag gggcaattgt tagaccagca aggccttttc
    34801 ctgatatacg caagaattct aatcgcgtta agccaatatc taatagtgga cgatcaaccg
    34861 ggttcattta tttcgcatcc tcttcgcaaa cacagtgctg acaattcagc agcctgatat
    34921 taagcattca actaatacgc tgctgactct gtccccaaca ccagatattt tccctaaccc
    34981 gatagtgtat gagaaaagat ttaacgccga taaactaatc ctttatggtt aggagaaaaa
    35041 ataatgtgat tattctgagc ccttaacatt gatcgttatc aattaaaatt acaaagcaag
    35101 aaattataag cgaactgata tttattatca tttgaaataa atttaactta agcgacaatc
    35161 atttgtgacg ttcattgcag aaaataaaaa taacattatc gctataaatt aaaatacata
    35221 ccgaaattcg tataatttca gcagttgtta tttagaatga ttaactggac cgcaactgaa
    35281 gcaagaatat caggatgaag cttaccgcct catcctgaat ggtcatttta ccagtgcgtt
    35341 aatgacggtt tccatcgcct gcttttccag ctcactgcgc tgaatgcgtt tattggctaa
    35401 tgctgtacgt aatacgcctg cgataacaat atgctttggt tcctgcccca gatcgcgcat
    35461 ttccagcaca actttaccga caactctgca catttctcgg tacagttcat cctcttttgt
    35521 ccgatttccc attctttata cctatttatc atgccaa
//
LOCUS       DAZPFP010000024        34800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPFP010000024 DAZPFP010000000
VERSION     DAZPFP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.024"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHUR4_001131"
     CDS             <1..2098
                     /locus_tag="ACHUR4_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1454799.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHUR4_001132"
     CDS             2095..3183
                     /locus_tag="ACHUR4_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1454800.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUR4_001133"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUR4_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR1454801.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUR4_001134"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUR4_001134"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1454802.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUR4_001135"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUR4_001135"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1454803.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHUR4_001136"
     CDS             8051..8602
                     /locus_tag="ACHUR4_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1454804.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUR4_001137"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUR4_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1454805.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUR4_001138"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUR4_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1454806.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHUR4_001139"
     CDS             complement(11683..12681)
                     /locus_tag="ACHUR4_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1454807.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHUR4_001140"
     CDS             complement(12760..13452)
                     /locus_tag="ACHUR4_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1454808.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHUR4_001141"
     CDS             complement(13529..14032)
                     /locus_tag="ACHUR4_001141"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1454809.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUR4_001142"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUR4_001142"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1454810.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHUR4_001143"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHUR4_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1454811.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHUR4_001144"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHUR4_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1454812.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUR4_001145"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUR4_001145"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1454813.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUR4_001146"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUR4_001146"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1454814.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUR4_001147"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUR4_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHUR4_001148"
     CDS             complement(22127..23560)
                     /locus_tag="ACHUR4_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1454815.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHUR4_001149"
     CDS             complement(23623..24072)
                     /locus_tag="ACHUR4_001149"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1454816.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUR4_001150"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUR4_001150"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR1454817.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUR4_001151"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUR4_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1454818.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHUR4_001152"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHUR4_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUR4_001153"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUR4_001153"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1454819.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHUR4_001154"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHUR4_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUR4_001155"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUR4_001155"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1454820.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUR4_001156"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUR4_001156"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACHUR4_001157"
     tRNA            complement(34082..34157)
                     /locus_tag="ACHUR4_001157"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACHUR4_001158"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACHUR4_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8948 c         9143 g         8221 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttcctgcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
//
LOCUS       DAZPFP010000025        34639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPFP010000025 DAZPFP010000000
VERSION     DAZPFP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.025"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHUR4_001159"
     CDS             complement(<1..1101)
                     /locus_tag="ACHUR4_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1454821.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUR4_001160"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUR4_001160"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1454822.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUR4_001161"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUR4_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1454823.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUR4_001162"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUR4_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1454824.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUR4_001163"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUR4_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1454825.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUR4_001164"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUR4_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1454826.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUR4_001165"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUR4_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1454827.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUR4_001166"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUR4_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR1454828.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUR4_001167"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUR4_001167"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1454829.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUR4_001168"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUR4_001168"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1454830.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUR4_001169"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUR4_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1454831.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUR4_001170"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUR4_001170"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1454832.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUR4_001171"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUR4_001171"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1454833.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHUR4_001172"
     CDS             11851..12015
                     /locus_tag="ACHUR4_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454834.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUR4_001173"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUR4_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1454835.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHUR4_001174"
     CDS             13524..13769
                     /locus_tag="ACHUR4_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1454836.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUR4_001175"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUR4_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1454837.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUR4_001176"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUR4_001176"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1454838.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUR4_001177"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUR4_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1454839.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUR4_001178"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUR4_001178"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1454840.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUR4_001179"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUR4_001179"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1454841.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHUR4_001180"
     CDS             19432..19785
                     /locus_tag="ACHUR4_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1454842.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUR4_001181"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUR4_001181"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1454843.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHUR4_001182"
     CDS             21085..22212
                     /locus_tag="ACHUR4_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR1454844.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUR4_001183"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUR4_001183"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1454845.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHUR4_001184"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHUR4_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUR4_001185"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUR4_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUR4_001186"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUR4_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1454846.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUR4_001187"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUR4_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1454847.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHUR4_001188"
     CDS             26834..27583
                     /locus_tag="ACHUR4_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1454848.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUR4_001189"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUR4_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1454849.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUR4_001190"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUR4_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1454850.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUR4_001191"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUR4_001191"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1454851.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUR4_001192"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUR4_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1454852.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUR4_001193"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUR4_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1454853.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUR4_001194"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUR4_001194"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1454854.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUR4_001195"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUR4_001195"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1454855.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..>34639)
                     /gene="acnA"
                     /locus_tag="ACHUR4_001196"
     CDS             complement(32673..>34639)
                     /gene="acnA"
                     /locus_tag="ACHUR4_001196"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1454856.1"
                     /translation="LGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     TLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASE
                     PIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8166 a         9029 c         8746 g         8698 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaaca
//
LOCUS       DAZPFP010000026        34325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPFP010000026 DAZPFP010000000
VERSION     DAZPFP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.026"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(9..152)
                     /gene="ypaB"
                     /locus_tag="ACHUR4_001197"
     CDS             complement(9..152)
                     /gene="ypaB"
                     /locus_tag="ACHUR4_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1454857.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            280..2565
                     /gene="nrdA"
                     /locus_tag="ACHUR4_001198"
     CDS             280..2565
                     /gene="nrdA"
                     /locus_tag="ACHUR4_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1454858.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2799..3929
                     /gene="nrdB"
                     /locus_tag="ACHUR4_001199"
     CDS             2799..3929
                     /gene="nrdB"
                     /locus_tag="ACHUR4_001199"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1454859.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3929..4183
                     /gene="yfaE"
                     /locus_tag="ACHUR4_001200"
     CDS             3929..4183
                     /gene="yfaE"
                     /locus_tag="ACHUR4_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1454860.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4237..4887)
                     /gene="inaA"
                     /locus_tag="ACHUR4_001201"
     CDS             complement(4237..4887)
                     /gene="inaA"
                     /locus_tag="ACHUR4_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR1454861.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4967..5071)
                     /locus_tag="ACHUR4_001202"
                     /pseudo
     CDS             complement(4967..5071)
                     /locus_tag="ACHUR4_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5066..5308
                     /locus_tag="ACHUR4_001203"
                     /pseudo
     CDS             5066..5308
                     /locus_tag="ACHUR4_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5350..6426)
                     /gene="glpQ"
                     /locus_tag="ACHUR4_001204"
     CDS             complement(5350..6426)
                     /gene="glpQ"
                     /locus_tag="ACHUR4_001204"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1454862.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6431..7789)
                     /gene="glpT"
                     /locus_tag="ACHUR4_001205"
     CDS             complement(6431..7789)
                     /gene="glpT"
                     /locus_tag="ACHUR4_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1454863.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8062..9690
                     /gene="glpA"
                     /locus_tag="ACHUR4_001206"
     CDS             8062..9690
                     /gene="glpA"
                     /locus_tag="ACHUR4_001206"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1454864.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9680..10939
                     /gene="glpB"
                     /locus_tag="ACHUR4_001207"
     CDS             9680..10939
                     /gene="glpB"
                     /locus_tag="ACHUR4_001207"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1454865.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            10936..12126
                     /gene="glpC"
                     /locus_tag="ACHUR4_001208"
     CDS             10936..12126
                     /gene="glpC"
                     /locus_tag="ACHUR4_001208"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1454866.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12319..13269
                     /locus_tag="ACHUR4_001209"
     CDS             12319..13269
                     /locus_tag="ACHUR4_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1454867.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13310..13771)
                     /locus_tag="ACHUR4_001210"
                     /pseudo
     CDS             complement(13310..13771)
                     /locus_tag="ACHUR4_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(13835..15037)
                     /locus_tag="ACHUR4_001211"
     CDS             complement(13835..15037)
                     /locus_tag="ACHUR4_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1454868.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15124..15666)
                     /locus_tag="ACHUR4_001212"
     CDS             complement(15124..15666)
                     /locus_tag="ACHUR4_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1454869.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            15945..16370
                     /gene="nudI"
                     /locus_tag="ACHUR4_001213"
     CDS             15945..16370
                     /gene="nudI"
                     /locus_tag="ACHUR4_001213"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1454870.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16409..17011)
                     /gene="ais"
                     /locus_tag="ACHUR4_001214"
     CDS             complement(16409..17011)
                     /gene="ais"
                     /locus_tag="ACHUR4_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1454871.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17319..18458
                     /gene="arnB"
                     /locus_tag="ACHUR4_001215"
     CDS             17319..18458
                     /gene="arnB"
                     /locus_tag="ACHUR4_001215"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1454872.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18462..19430
                     /gene="arnC"
                     /locus_tag="ACHUR4_001216"
     CDS             18462..19430
                     /gene="arnC"
                     /locus_tag="ACHUR4_001216"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1454873.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19430..21411
                     /gene="arnA"
                     /locus_tag="ACHUR4_001217"
                     /pseudo
     CDS             19430..21411
                     /gene="arnA"
                     /locus_tag="ACHUR4_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21408..22298
                     /gene="arnD"
                     /locus_tag="ACHUR4_001218"
     CDS             21408..22298
                     /gene="arnD"
                     /locus_tag="ACHUR4_001218"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1454874.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22298..23950
                     /gene="arnT"
                     /locus_tag="ACHUR4_001219"
     CDS             22298..23950
                     /gene="arnT"
                     /locus_tag="ACHUR4_001219"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1454875.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            23947..24282
                     /gene="arnE"
                     /locus_tag="ACHUR4_001220"
     CDS             23947..24282
                     /gene="arnE"
                     /locus_tag="ACHUR4_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1454876.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24282..24668
                     /gene="arnF"
                     /locus_tag="ACHUR4_001221"
     CDS             24282..24668
                     /gene="arnF"
                     /locus_tag="ACHUR4_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1454877.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24662..24934)
                     /gene="pmrD"
                     /locus_tag="ACHUR4_001222"
     CDS             complement(24662..24934)
                     /gene="pmrD"
                     /locus_tag="ACHUR4_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1454878.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25039..26394)
                     /gene="menE"
                     /locus_tag="ACHUR4_001223"
     CDS             complement(25039..26394)
                     /gene="menE"
                     /locus_tag="ACHUR4_001223"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1454879.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26391..27353)
                     /gene="menC"
                     /locus_tag="ACHUR4_001224"
     CDS             complement(26391..27353)
                     /gene="menC"
                     /locus_tag="ACHUR4_001224"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1454880.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27353..28210)
                     /gene="menB"
                     /locus_tag="ACHUR4_001225"
     CDS             complement(27353..28210)
                     /gene="menB"
                     /locus_tag="ACHUR4_001225"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1454881.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28225..28983)
                     /gene="menH"
                     /locus_tag="ACHUR4_001226"
     CDS             complement(28225..28983)
                     /gene="menH"
                     /locus_tag="ACHUR4_001226"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1454882.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(28980..30650)
                     /gene="menD"
                     /locus_tag="ACHUR4_001227"
     CDS             complement(28980..30650)
                     /gene="menD"
                     /locus_tag="ACHUR4_001227"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1454883.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30739..32034)
                     /gene="menF"
                     /locus_tag="ACHUR4_001228"
     CDS             complement(30739..32034)
                     /gene="menF"
                     /locus_tag="ACHUR4_001228"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1454884.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32113..32418)
                     /gene="elaB"
                     /locus_tag="ACHUR4_001229"
     CDS             complement(32113..32418)
                     /gene="elaB"
                     /locus_tag="ACHUR4_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1454885.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32473..32934)
                     /locus_tag="ACHUR4_001230"
     CDS             complement(32473..32934)
                     /locus_tag="ACHUR4_001230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1454886.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            32999..33916
                     /gene="rbn"
                     /locus_tag="ACHUR4_001231"
     CDS             32999..33916
                     /gene="rbn"
                     /locus_tag="ACHUR4_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1454887.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34107..34295
                     /locus_tag="ACHUR4_001232"
                     /pseudo
     CDS             34107..34295
                     /locus_tag="ACHUR4_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8334 a         9234 c         8559 g         8198 t
ORIGIN      
        1 tctgccgttc aagaaatcgc cgaacagtta tttttaacaa atttttctct tcccattgac
       61 tttcccggac accttgtctg acctaaggtg cgcgaaagcc actttttcct tcctgagtta
      121 tccacaaagt tatgcacttg caagagggtc attttcacac tatcttgcag tgaatcccaa
      181 acataccccc tatatatagt gttctaagca gcttccctta ctacaggtag tctgcatgaa
      241 actattgcgg aaagaattcc aaaaacaggt acgacataca tgaatcagaa tctgctggtg
      301 acaaagcgcg acggtagcac agagcgcatc aatctcgaca aaatccatcg cgttctggat
      361 tgggcggcag aaggactgca taacgtttcg atttcccagg tcgagctgcg ctcccacatt
      421 cagttttatg acggtatcaa gacctctgac atccacgaaa ctattatcaa ggctgccgca
      481 gacctgatct cccgtgatgc gccggattat cagtatctcg ccgcgcgcct ggcgatcttc
      541 cacctgcgta aaaaagccta cggccagttt gagccgcctg cgctgtacga ccacgtggtg
      601 aaaatggtcg agatgggcaa atacgataat catctgctgg aagactacac ggaagaagag
      661 ttcaagcaga tggacacgtt tatcgatcac gaccgtgata tgaccttctc ttatgctgcc
      721 gttaagcagc tggaaggcaa atatctagta cagaaccgcg tgaccggcga aatctacgaa
      781 agcgcccagt tcctttatat tcttgttgcc gcgtgcctgt tctcgaacta cccgcgtgaa
      841 acgcgcctgc aatacgtgaa gcgtttttat gacgcggttt ccacatttaa aatttcgctg
      901 ccgacgccaa tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc ctgcgtactg
      961 atcgagtgcg gtgacagcct ggattccatc aacgccacct ccagcgcgat tgtgaaatat
     1021 gtttcccagc gtgccgggat cggcatcaac gccggacgta ttcgtgcgct gggcagcccg
     1081 attcgcggtg gcgaagcgtt ccataccggc tgtatcccgt tctacaaaca tttccagaca
     1141 gcggtgaaat cctgctctca gggcggtgtg cgcggcggtg cggcaacgct gttctacccg
     1201 atgtggcatc tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg ggttgaaggc
     1261 aaccgcgtgc gtcatatgga ctacggggta caaatcaaca aactgatgta tacccgcctg
     1321 ctgaaaggtg aagatatcac cctgttcagc ccgtccgacg taccggggct gtacgacgcg
     1381 ttcttcgccg atcaggaaga gtttgaacgc ctgtatacca aatatgagaa agacgacagc
     1441 atccgcaagc agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca ggaacgtgcg
     1501 tctaccggtc gtatctatat tcagaacgtt gaccactgta atacccatag tccgtttgat
     1561 ccgaacatcg cgccagttcg ccagtctaac ctgtgcctag aaattgccct gccgaccaaa
     1621 ccgctgaacg acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct gtctgctttc
     1681 aacctgggcg caattaatag cctggatgaa ctggaagagc tggcgattct ggcggttcgt
     1741 gcacttgacg cgctactgga ttatcaggat tacccgatcc cggccgccaa acgtggagcg
     1801 atggggcgtc gtacgctggg tattggtgtg attaacttcg cttactatct ggcgaagcac
     1861 ggtaaacgct actccgacgg cagcgccaac aacctgacgc ataaaacctt cgaagcgatt
     1921 cagtattacc tgctgaaagc gtctaatgag ctggctaaag agcaaggcgc gtgcccgtgg
     1981 tttaacgaaa ccacttacgc gaaagggatc ctgccgatcg atacctataa gaaagatctg
     2041 gatgccatcg ctaatgagcc gctgcattac gactgggaag cattgcgtga atcaatcaaa
     2101 acgcacggcc tgcgtaactc cacgctttct gccctgatgc cgtccgagac ctcttcgcag
     2161 atctctaacg ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat caaagcgtcg
     2221 aaagacggta ttttgcgcca ggtggtgccg gactacgagc acctgcacga cgcctatgag
     2281 ctgctgtggg aaatgccggg taacgatggc tacctgcaac tggtaggtat catgcagaaa
     2341 tttatcgatc agtcgatctc tgctaacacc aactacgatc cgtcacgttt cccgtcagga
     2401 aaagtgccga tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt cggggtcaaa
     2461 acgctgtatt atcagaacac ccgtgacggc gctgaagacg cgcaggacga tctggtaccg
     2521 tcaatccagg acgatggctg cgaaagcggc gcatgtaaga tctgatattg agatgccgga
     2581 tgcggcgtaa acgccttatc cggcctacgg ctcggtttgt aggcctgata agacgcgcca
     2641 gcgtcgcatc aggctccggg tgccggatgc agcgtgaacg ccttatccgg cctacggctc
     2701 ggatttgtag gcctgataag acgcgccagc gtcgcatcag gcacaggatg cggcgtaaaa
     2761 tgccttatcc ggcattaaac tcccaacagg acacactcat ggcatatacc accttttcac
     2821 agacgaaaaa tgatcagctc aaagaaccga tgttctttgg tcagccggtc aacgtggctc
     2881 gctacgatca gcaaaaatat gacatcttcg aaaagctgat cgaaaagcag ctctctttct
     2941 tctggcgtcc ggaagaagtt gacgtctccc gcgaccgtat agattaccag gcgctgccgg
     3001 agcacgaaaa acacatcttt atcagcaacc tgaaatatca gacgctgctg gattccattc
     3061 agggtcgtag cccgaacgtg gcgctattgc cgcttatttc tattccggaa ctggaaacct
     3121 gggtcgaaac ctgggcgttc tcagaaacga ttcattcccg ttcctatact catatcattc
     3181 gtaatatcgt taacgatccg tctgttgtgt ttgacgatat cgtcaccaac gagcagatcc
     3241 agaaacgtgc ggaagggatc tccagctatt acgatgaact gatcgaaatg accagctact
     3301 ggcaccttct gggcgaaggc acccacaccg ttaacggtaa aaccgtaacc gttagcctgc
     3361 gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt taacgcgctg gaagcgatcc
     3421 gcttctacgt cagctttgct tgttcctttg cctttgcaga acgcgaactg atggaaggta
     3481 acgccaagat tattcgcctg attgcccgcg atgaagccct gcacctgacc ggtactcagc
     3541 atatgctgaa tctgctgcgc agcggcgcgg atgatccgga gatggcggaa attgccgaag
     3601 aatgtaagca ggagtgctat gacctgtttg tccaggcggc acagcaagag aaagactggg
     3661 cggattatct gttccgtgac ggttcgatga ttggtctgaa taaagatatt ctctgccagt
     3721 acgttgaata catcaccaat atccgcatgc aggcagtcgg tttggatctg ccgttccaga
     3781 cgcgctccaa cccgatcccg tggatcaaca cctggctggt gtctgataac gtgcaggttg
     3841 ctccgcagga agtggaagtc agttcttatc tggtcgggca gattgacgcg gaagtggaca
     3901 ccgacgattt gagtaacttc cagctctgat ggcccgcgtt accctgcgca tcactggcac
     3961 acaactgctg tgccaggatg aacacccttc ccttctggcg gcgctggaat cccacaatgt
     4021 ggcggttgag taccagtgcc gcgaaggtta ctgcggctcc tgtcgcaccc gactggtcgc
     4081 aggtcaggtg gactggattg ccgaaccgtt agcctttatt cagccggggg aaattttgcc
     4141 ctgttgttgc cgggcaaaag gcgatattga aatcgagttg tgatcgggta ctagcgccag
     4201 atacgacgct tacgcgtatt atctggccta caacgattac atggcgtagt aatacgcttt
     4261 cacctgctcc cagtccgctt tagggatcgg ctccagatat ttttccagct ggcggaagtc
     4321 atgattaatc gctttatcgc gacgcaagcg gcgacgactt ttctccagat caagaaaacc
     4381 agcttctgcc ttaccttctg ttttcacata gatatggcga acatagcaac agccatgctg
     4441 acgattaacg ctatgcattt tcttaaacgc cagtgccact gctttcaaca tggcttgccg
     4501 tacttcgtca gaataaggcg atactgcatg ccgggcatac cagtcagcaa tgctgatgaa
     4561 ccccgccata tcttcagtca ccagcaacgc gcgccattca ccctcaattt tcaccgcttc
     4621 accaaaaacg atcttcggca cgatgacacc ggcccgttcc agttctttaa ttaccgcaac
     4681 ctcacggaca atcgttggtc ggccgaacgg ataacgtact gaatgaaaca gatgatgcgt
     4741 catgcgcttt acatacagct ttttgccgtt gcgctcgacg cattgcaccc cgctcatacc
     4801 attacggcga tagttaggct cttcaaccca gtcgccctct gttgcccacc agtgattaaa
     4861 ttcgtcgtac tttgctgaaa ctgccatacc ctatcgcctg tcatttttat taacgacaat
     4921 gactataggt ggttacctga ggaaaatctt aatgaaacgt gtcgtattaa tgaatttctt
     4981 ttgccgctac aggtgtgaca ccgcttttgc ccttcatttg cagcgcatag attccaccag
     5041 ccactaccac taatagtgcg cccagcagat aaaatgtgaa tttaatcagt cggatacagc
     5101 gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa cgctgaaaag
     5161 cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac agatttcgtt
     5221 gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa tgtgcatgga
     5281 acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga acgccatcct
     5341 gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat cagtaaacag
     5401 cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat caggagtgta
     5461 ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat tctgctgggc
     5521 atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct caatcaacat
     5581 atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg ctcccggctt
     5641 aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct gctgcgtttc
     5701 attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca ttttgggttc
     5761 cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat aaactttatc
     5821 gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg caatatcctt
     5881 cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga tatttttccc
     5941 ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt gcacccggaa
     6001 gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac cgttttcaat
     6061 atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat ctatcgcgta
     6121 gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag taacacgatc
     6181 gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca aatcctgttc
     6241 cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat gctccggcaa
     6301 atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg ccgccattgc
     6361 actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt tcagcttcaa
     6421 tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga cgtttttcgc
     6481 caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc attaccataa
     6541 agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc gccgccaccg
     6601 aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc gcttttttcg
     6661 gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc aggaagccga
     6721 taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc cagtaaacga
     6781 tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg ttaccacgga
     6841 agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca tactcataga
     6901 agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc agataagtcg
     6961 gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg atggcgatgt
     7021 accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg gtcagctcct
     7081 gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg atcggcggca
     7141 agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc accagaatgg
     7201 cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc atccccagca
     7261 ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac actgacacaa
     7321 tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac ggcggccacc
     7381 ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca atgctcgacg
     7441 tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc aaacctgcgg
     7501 gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat ttcgaaaatc
     7561 cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag aatccctgct
     7621 caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa gccgcatagc
     7681 caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga tcgatctccg
     7741 cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt gatagcctcc
     7801 gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc tgccgtttct
     7861 tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc gcacacatta
     7921 ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta ctttcgaatc
     7981 atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa atggtaaaaa
     8041 acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga cgtgattatc
     8101 attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg cgggctgcgc
     8161 gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa ccacggcctg
     8221 ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga atgcattagt
     8281 gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa cggcctgttt
     8341 atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc ctgcgaagaa
     8401 gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga acctgccgtt
     8461 aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc atttcgtctg
     8521 accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac cgctcatgaa
     8581 gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg taaccatctc
     8641 accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg gatctggggg
     8701 caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa aggatcgctg
     8761 ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa accttccgac
     8821 gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc tttacgtatt
     8881 gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat tctgctgcgt
     8941 gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc ctattctggc
     9001 gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag ccgtggcatc
     9061 gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat caccggtggc
     9121 aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg ccgcaaactg
     9181 ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca agaacccgct
     9241 gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc tgcggtttat
     9301 cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg tagcctggta
     9361 tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa tttaaacgtt
     9421 aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg ccagggcgaa
     9481 ctctgcgcct gccgcgctgc cggactgctg caacggttta acgtcacgac gtccgcgcaa
     9541 tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca acccatcgcc
     9601 tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg actgtgtggt
     9661 ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggcg gcctcgccgg
     9721 attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg tcacgcgtgg
     9781 tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc tgccagatgg
     9841 tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg cacctgcaca
     9901 tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg cgcaggcatt
     9961 aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc agcggattac
    10021 gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg tctggccgct
    10081 acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc aggcgcatct
    10141 tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa tagagctgcc
    10201 ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata tcgcccgttt
    10261 ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg tcgccaatac
    10321 ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac tgtggcgttg
    10381 gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc cttccgtgct
    10441 gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg gagtgtggat
    10501 gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg aaatctggac
    10561 ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca gcggcagttt
    10621 ctttagtggc ggactagtag cggaacgtaa cggcattcgc gagccgattc tcggccttga
    10681 tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg cgccgcaacc
    10741 gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg cagggcaaac
    10801 cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca tcgcccaggg
    10861 atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga ttgcccaacg
    10921 cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg caccgtctgc
    10981 accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa acaagccggg
    11041 ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc gctgaaatat
    11101 tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat tggcgatatt
    11161 atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa ttttgtgttg
    11221 agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt caacaccgct
    11281 acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga tcatcgtcgc
    11341 acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat agcggctcag
    11401 caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa ctacaaccat
    11461 ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg tgtacaactg
    11521 ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac cgataaagca
    11581 cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt aaaaggcatt
    11641 ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata cccggaagtg
    11701 ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg ctggctgtgg
    11761 cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa agtggtttat
    11821 cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga gctgttgcgt
    11881 aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat tgcgggtact
    11941 tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc actgttccgc
    12001 cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg taaatggcag
    12061 attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc ccaggcgctg
    12121 gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt tgcgtacacg
    12181 attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa cgtctcgcaa
    12241 aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt catcataaat
    12301 atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga tgcggtattt
    12361 aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca tttaccagaa
    12421 ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag ttttattgaa
    12481 aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag cgacggtgac
    12541 ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat ggcttttcgg
    12601 ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta tgacagagtt
    12661 ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc gtactcactt
    12721 aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac cgaagctttt
    12781 ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg gcgtatagct
    12841 ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat ggtcgacagg
    12901 atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac actgtttaat
    12961 tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga gattgccgaa
    13021 cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg gcaggaagga
    13081 catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa attagaaggt
    13141 ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct ggaacagggc
    13201 attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct ggcagcgaat
    13261 aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat caataactac
    13321 cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga tccagggcat
    13381 cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac gccaggcatt
    13441 gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca gcacggatcc
    13501 gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta tccgggtagc
    13561 ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct tcgacgtcga
    13621 ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac agacaaagcg
    13681 aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc cgtgccacac
    13741 tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg agggggaatg
    13801 tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc atggactcaa
    13861 caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa cggcgtagca
    13921 tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag cgtgtagtgc
    13981 tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag ttgagatgct
    14041 cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc cgccgttctg
    14101 taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact tcacacgcca
    14161 gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg aattgctcgc
    14221 tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg ggcagtcctt
    14281 cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc cacagttttt
    14341 ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc ggcattgagg
    14401 agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt tgtgccagat
    14461 cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt ggtaaagaaa
    14521 aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat tctgacggta
    14581 cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca caggcggtgc
    14641 ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta cggttgctcg
    14701 gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc caggcttcat
    14761 gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa tcatcgctgg
    14821 tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt tcgcgaagaa
    14881 tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat ggcagcccct
    14941 gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc ccgtataaca
    15001 cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc ttttgttatc
    15061 gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc gcgccggaag
    15121 ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt tgtcgcggtt
    15181 accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga ctggacgcat
    15241 aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc tggagagcga
    15301 atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt tccactgcaa
    15361 accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg tgtacacgcc
    15421 ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca gccccacgcc
    15481 cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca ggcccgtcga
    15541 ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc cgctgatgct
    15601 cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta gcgcaatttt
    15661 tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc gggattgtac
    15721 tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat tgtaactaaa
    15781 tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac acacaaaaat
    15841 aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat atttctgagt
    15901 taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga caacggacta
    15961 ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg gccgacgatc
    16021 gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct ggcgaacgaa
    16081 ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg cttttgacag
    16141 aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca gatggtcgca
    16201 aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac cgagaagtga
    16261 aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg gtgcattatg
    16321 atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa caatgatgac
    16381 agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca tccagataaa
    16441 ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta aatatcgcat
    16501 cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata acgatatttt
    16561 tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta ccgcactgaa
    16621 gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc gactgaatgg
    16681 tccggacggt attactggaa taaagatcga aatcagagat gtcagcgcta aaagcgttgc
    16741 ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct gacaagcatt
    16801 gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc gggtgctgct
    16861 gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta ctccaggcgg
    16921 catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa tatttttttg
    16981 acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac aagcgcaacc
    17041 agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg gtgtaatatt
    17101 aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa gcctaaataa
    17161 gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg ttaagttaat
    17221 attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct ttatagcaaa
    17281 tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt ttgccttttt
    17341 cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc gaatccggtt
    17401 ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa
    17461 atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg ctaatggcgt
    17521 tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt tcaaccctca
    17581 atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc gatacgctga
    17641 tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc atcattccgg
    17701 tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc gaacgttacg
    17761 gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa gggcgacata
    17821 ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt acctgtgctg
    17881 aaggtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg atgctgaaat
    17941 ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca ccgcaggctg
    18001 aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg attgccctga
    18061 cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc
    18121 agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg ccgcacgttc
    18181 acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg
    18241 cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc cgcgccgctc
    18301 acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat accgaatgga
    18361 atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc gatgccgacc
    18421 gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa atccaccctg
    18481 ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta ccggaattaa
    18541 tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg
    18601 atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa gcggagaaca
    18661 gccatattgt gtctattttg attaaccgca attacgggca acattcagcg attatggcgg
    18721 gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc cagaatccgc
    18781 cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg gtagggactg
    18841 tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg attaaccggc
    18901 ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc
    18961 gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt atcccgattc
    19021 tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc gagcgtgagt
    19081 ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac ctggtgacct
    19141 gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt gcgattggag
    19201 gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca caatgggcgg
    19261 cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct cagtttatcg
    19321 gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc gcccgccccc
    19381 gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa tgaaaaccgt
    19441 cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg ctgccggtta
    19501 cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct tttatggttc
    19561 ggtggctcat ctggcggcgg aaagagacat tccggtttat gcgccggata acgttaatca
    19621 tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct ctttttatta
    19681 tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat ttaatctgca
    19741 cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc tggtgaacgg
    19801 tgaaacggaa acgggcgtta cattgcaccg aatggtaaac gtgccgatgc tggggccatt
    19861 gtggcgcaac tgcgcgttgc cattgcgcca gacgatatcg ctattacgct gcatcataaa
    19921 ttgtgccatg ccgcgcgcca gctactggaa cagacattac ccgccattaa acacggtaat
    19981 attctggaaa tcgcccagcg cgaaaacgaa gccacctgtt ttggtcgcag aacgccggat
    20041 gacagtttcc ttgaatggca taaaccggca tccgtactgc acaacatggt acgtgccgtt
    20101 gccgatccgt ggccgggtgc cttcagctat gttggcaatc agaaattcac cgtctggtcg
    20161 tcgcgtgttc atcctcatgc cagcaaagca cagccgggga gcgtgatttc tgttgcgcca
    20221 ctgctgattg cctgtggcga tggcgcgctg gaaatcgtca ccggacaggc gggcgacggc
    20281 attactatgc agggctcgca attagcgcag atgctgggcc tggtgcaagg ttcacgcttg
    20341 aatagccagc ctgcctgcac cgcccgacgc cgtacccggg tactcatcct cggggtgaat
    20401 ggctttattg gcaaccatct gacagaacgc ctgctgcgcg aagatcatta tgaagtttac
    20461 ggtctggata ttggcagcga tgcgataagc cgttttctga atcatccaca ttttcacttt
    20521 gtcgaaggcg atatcagtat tcattccgaa tggattgagt atcacgtcaa aaaatgtgat
    20581 gtcgtcttgc cgctggtggc gatagccacg ccgattgaat atacccgcaa cccgctgcgc
    20641 gtatttgaac tcgattttga agagaatctg cgcattatcc gctactgcgt taagtaccgt
    20701 aagcgaatca tcttcccgtc aacttcagaa gtttatggga tgtgtagcga taaatacttc
    20761 gatgaggacc attctaattt aatcgtcggc ccggtgaata aaccacgctg gatttattcg
    20821 gtgtcaaaac aattacttga tcgggtgatc tgggcctatg gcgaaaaaga gggtttacag
    20881 ttcaccctct tcctcccgtt taactggatg ggaccacgac tggataacct taatgcagcg
    20941 cgaattggca gctcccgcgc tattacgcaa ctcattctca atctggtaga aggttcacca
    21001 attaagctaa ttgatggcgg aaaacaaaaa cgctgcttta ctgatatccg ggacggtatc
    21061 gaggcgttgt accacattat cgaaaatgcg ggaaatcgct gcgacggcga aattatcaac
    21121 attggcaatc ctgagaacga agcgagcatt gaggaactgg gcgagatgct gctggcgagc
    21181 ttcgaaaaac atccgctgcg ccatcatttc ccaccgtttg cgggctttcg cgttgtcgaa
    21241 agtagctgct actacggcaa aggatatcag gacgtagagc atcgtaaacc gagcatccgc
    21301 aatgcccacc gctgcctgga ctgggagccg aaaattgata tgcaggaaac catcgacgaa
    21361 acgctggatt tcttcctgcg caccgttgat cttacggata aaccatcatg accaaagtag
    21421 gcttacgcat tgatgtcgat gcctttcgtg gcacccgtga aggcgtgccg cgtctgctgg
    21481 aaatcttgag taagcataat attcaggcca gcattttttt cagcgtcggc ccggacaata
    21541 tgggccgcca tctctggcga ctggtgaagc cacagttttt gtggaagatg ctgcgctcaa
    21601 acgcggcatc gctttatggc tgggatattt tactggcagg tacggcctgg ccaggtaaag
    21661 agattggtca tgccaatgcc gatatcattc gtgaagcggc taaacatcac gaagtcggtc
    21721 tgcacgcctg ggatcaccat gcctggcaag cccgtagcgg taactgggat cggcaaacaa
    21781 tgatcgacga tattgcacgc ggtcttcgca ctctggaaga gattatcggt caaccggtaa
    21841 cctgttctgc cgctgcgggc tggcgtgccg accagaaggt gatcgaagca aaagaagcgt
    21901 tccatttgcg ctacaacagc gattgtcgtg gggccatacc gttccgtcca ctgcttgaat
    21961 caggaaaccc tggcactgcg caaattccgg tgaccttacc cacctgggat gaagtgattg
    22021 gtcgggatgt gaaagcagaa gattttaacg gttggttact caaccgcatc ctgcgagata
    22081 aaggcacgcc ggtttatacc attcatgcag aagttgaatg ctgcgcttat cagcataatt
    22141 ttgtggatct cctcaaacgc gcagctcagg aaggcgtgac attttgccct ttaagcgaac
    22201 tgttatcaga gacgttgccg ctcggacaag ttgttcgcgg aaatattgcc ggacgtgaag
    22261 gctggctggg ttgccaacaa attgcgggta gtcgctgatg aaatcggtac gttaccttat
    22321 cggcatcttc gcgtttattg cctgctatta cctgttaccg atcagcacgc gtctgctctg
    22381 gcaacccgat gaaacgcgtt atgcggaaat cagtcgagaa atgctggcat ccggcgactg
    22441 gattgtgccc catctgttag ggctacgtta tttcgaaaaa cccattgccg gatactggat
    22501 taacagcatt gggcaatggc tatttggcgc gaataacttt ggtgtgcggg caggcgttat
    22561 ctttgcgacc ctgttaactg ccgcgctggt gacctggttt actctgcgct tatggcgcga
    22621 taaacgtctg gctctactcg ccacagtaat ttatctctca ttgtttattg tctatgccat
    22681 cggcacttat gccgtgctcg atccgtttat tgcattctgg ctggtggcgg gaatgtgcag
    22741 cttctggctg gcaatgcagg cacagacgtg gaaaggcaaa agcgcaggat ttttactgct
    22801 gggaatcacc tgcggcatgg gggtgatgac caaaggtttt ctcgcccttg ccgtgccggt
    22861 attaagcgtg ctgccatggg tagcgacgca aaaacgctgg aaagatctct ttatttacgg
    22921 ctggctggcg gttatcagtt gcgtactgac ggttctcccc tggggactgg cgatagcgca
    22981 gcgggagcct gacttctggc actatttttt ctgggttgag catattcaac gctttgcact
    23041 agatgatgcc caacatagag ctccgttctg gtactacgtg ccggtcatca ttgccggtag
    23101 cctgccgtgg ctgggattac tccccggtgc actgtacaca ggctggaaaa accgcaagca
    23161 ttccgcaacc gtctatttgt tgagctggac gataatgccg ctgctgtttt tctccgtcgc
    23221 taaaggcaaa ttgcccacct atattctttc ttgctttgca cctctggcaa tgctgttggc
    23281 gcattacgcg ttgctggcag caaaaaataa tcctctggcg ctgcggatta atggctggat
    23341 taacatcgct tttggcgtca ctggcattat tgccacattt gtgatctccc cctggggacc
    23401 aatgaacacg ccggtgtggc aaaccttcga gagctataaa gtcttttgtg cctggtcgat
    23461 tttttcgcta tgggcatttt tcggctggta caccttaaca aacgtcgaaa agacctggtc
    23521 tttcgccgcg ctttgcccgc tggggctggc gttgctggta ggattttcaa ttcctgacag
    23581 agtcatggaa ggaaaacatc cgcaattttt tgtcgagatg acacaagaat cacttcagcc
    23641 aagccgctat attcttactg atagcgtcgg tgttgccgca ggtctggcat ggagcctgca
    23701 acgcgatgac atcatcatgt atcgccagac aggtgagttg aaatacggcc ttaattatcc
    23761 ggatgcgaaa gggagatttg tcagcggtga tgagttcgca aactggctta atcaacatcg
    23821 tcaggagggg attattacac tcgtgctttc ggttgaccgc gatgaagata tcaacagtct
    23881 cgccattccg cccgcagatg ccatcgttcg tcaggagcgt ctggtgctga ttcagtatcg
    23941 tcccaaatga tctggctaac attagtcttt gccagcttgc ttagcgttgc cgggcagttg
    24001 tgtcagaaac aggcaacctg ctttgtggcg ataaacaaac ggcgcaaaca tatcgtgctg
    24061 tggctgggac tggcgctggc ttgtcttggt cttgccatga tgctctggct gctggtcttg
    24121 cagaacgtac cggtaggcat tgcctacccg atgttaagtc tgaattttgt ctgggtgacg
    24181 ctggctgcag taaaactgtg gcacgaaccg gtatcgccgc gtcactggtg tggggtggcg
    24241 ttcattattg gtggcattgt gatcctcggg agtacggtgt aatgtgcctg atatggggat
    24301 tattcagcgt cattattgcc tcagttgcgc aattaagcct gggttttgcg gcaagtcatc
    24361 tgccgccgat gacgcactta tgggatttta tcgcggctct gttagctttt ggcctggatg
    24421 cccggatact gttactcggg ctgctgggtt atctgctttc cgttttttgt tggtataaaa
    24481 cgctacataa acttgcgcta agcaaagcct atgccttgct cagtatgagt tatgtgctgg
    24541 tatggattgc ttcaatggta cttcccggct gggaagggac tttttcgctg aaagcactac
    24601 tgggagtagc ctgtattatg agcgggttga tgctgatttt cctgcccacg acaaaacaac
    24661 gttactgagt tttccctgcc gctgtacact gccgttccca ctcatcgggg ctataactgc
    24721 ttgccgagag gactttcacc gtatgcagct tttcacgatt aatacaataa agcgcattct
    24781 gcaaaggcga gagtaaatcc cccactttca cggcgaagtc actcttcact tcggcgatca
    24841 tttttatcgc gccgccagca tcgcagagca taagtacatg tctgttatct tgtttattgc
    24901 agcacgattt tttgtgccag ccattccatt gcattatcct gtttgctaag agttttcaca
    24961 tcaatttaca gattaacaga taaactcttt atcaacggca atggaatggc agaaatgaga
    25021 agtgaaacgg agggttaatt attgctgacg ttgcacccac tcttttagcg cctgacgtga
    25081 aattttaata ccgccgtttt tcagctccgg cggtaaagtc agccagcgca ccggttgttg
    25141 aaaacgagcc agcttatctt tcacccattc gccaagatca acggtttgct ggtcatactc
    25201 caccaccgct accggccgat gaccaaactc cttgtcggca acggaaacga taaacacctg
    25261 caaaaccgca ggatgtgcag caataacacg ctcgacttct tccggctgaa taccctcacc
    25321 gccactgaag aacagattgt ctaaacgtcc gacaatggtc agcttgccat tatgcatctc
    25381 accgcgatcg cgcgtagcgt accagccttc gtcattaacc agtgaaacca gttgcccgtt
    25441 acgccagtaa ccttctgcca tactggcagc ccgcagccac acttcattat taacgatttt
    25501 cacttcccga cccggcagcg gcaaaccaac gtctgccagg ccgtcggctt ctttcgcaca
    25561 taccgtggag gcaaactcgg tcagaccata gccgcaaaag caacgaatcc cctgctcgcg
    25621 cgcctgttcc gtcaactcga ccgggatagc cgcgccgcca agtaacaccg ctttcaggga
    25681 aacggaactg cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt
    25741 acagcctgcc agcatttgct ccaatggctg tttatcacgc accgtcatcc gcgcaccagc
    25801 gtataaccag cgccacataa ttccctgacc ggagacgtga aacagcggta aagagagcaa
    25861 ccagtcatcg tgatcgccaa acggaatcag cgataacaca ccttgcgcac tggcaagatg
    25921 ggcctgataa gtatggacag cggctttcgg caaaccggta gaacccgagg tcaacgtcat
    25981 tgagcacaga cgcgtcggct gccacgcagc ggcatgtacg ccttcaacca gctgaatgtg
    26041 cagcgacgtt aatgccggaa acgtgttttc cccatccggc accagcgcaa attgcagcgt
    26101 cagattgggc agcaattctt caagcaacgg ttgcggcagc tgagggttca cgggcaacac
    26161 ccgcgccccg cattgcagta acgccagcca ggcgagcagc gtttgcggcg tattccacgc
    26221 ccgcaacatc acgccgctgc cctcaaccac cccctgcgcc gcaaagcccg tagctaactc
    26281 atcgacgcga gcacaaagct cgcgccagtt gagttgctcg tcattaagac gtaaggcgat
    26341 ggcttctccc cgcacttgcc gccagtgacg ccacggccag tcagagaaga tcataacaac
    26401 tgctcctgta catcagcgtc cactaacggc agtgggctcc ccggccagcg acgaacctgc
    26461 tgcgcctgca tcagatccag cgtgtccagt cccggaatgg tgtctggcgt taaccaggca
    26521 gcaatccgcg ccagttgcgt tagacctaag ctcgactcaa tggaagaact gatcaccgcc
    26581 gtcagcccca gcgcatgcgc cgcctgtacc tgctcgcgta ctttttccag actgcccgtg
    26641 agcgtgggtt tgataactac cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc
    26701 tcgcgcaggc tttcatccca ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa
    26761 tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata atccgggtta
    26821 acatatttgg caaactgctg acctttcagc ggtgtccagg cgcgatttgc gtcaagacgc
    26881 aaatgcagat ccggaattgc ctccagcaac agattcacca ccatgccgtc gcgcaccgct
    26941 tcgtacaatc ccactttgac cttcgccact ttctcgcccg gcatatcggc aagtttgagg
    27001 atcagatcgt ccggatcgcc attacacagc ggtgccgcac ggtagttggc tgcttgcggc
    27061 aacgtatctg tcagttctgc caatgcacag cttacgccaa aggccacgga aggcatctgc
    27121 ggtagctcgc aatcgcctgc cagccagtta tttacccagg caagcagcac gctttgcgcc
    27181 tcttcccagt tttcctggct gaagcccggc agtggggaga tctcccccca cccttcgcgc
    27241 tcgccctcac gcaggcaaac gtacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga
    27301 accacccccg cgtccatggg gatctgccag cggtatacct gcgcgctacg cattacggat
    27361 tccgtttaaa tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg cgaccttcct
    27421 gaccttcttc cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc tgcagccccg
    27481 cctgcccgtc acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt
    27541 tttgcagcat ttcgcggcac caacggacgg tttctttttc cagatccgcc agcggtacca
    27601 cggtgttgac aaggcccata tccagcgcct gttttgcgtc gtactgacgg cacaggaacc
    27661 agatttctcg cgcttttttc tgcccgacga tacgagccat gtaggaagcg ccccagccgc
    27721 cgtcgaagga accgactttc gggccagtct gaccgaagat ggcattatct gctgcgatag
    27781 tcaggtcgca catcatgtgc agaacgtgac caccgccgat ggagtagcca gccaccatcg
    27841 cgacaaccgg tttcggacag gtacggatct gacgctggaa gtccagcacg ttcaggtgat
    27901 gtacgccgga atcatcttta tagccgccgt aatcaccacg cactttctgg tcaccaccgg
    27961 agcagaacgc tttatcgcct gcgccagtca gaatgatcac gccgatattg tcgtcataac
    28021 gcgcatctgc cagtgcctgg atcatctctt ttaccgtcag aggacggaag gcattgcgca
    28081 cctgcggacg attaatggtg atttttgcga taccgtcggt ggatttttca taacgaatgt
    28141 cctcgaaacc ttcggagcag tcgtgccatt caaccggtgc gtaaggcatt gcttcatcag
    28201 gataaatcat attgtgtcct ttagtcagaa acgcaagatc tgcgccagac ttgcgattac
    28261 gccagcggga ttttcccgat gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc
    28321 agccagttcc gccgccaggg cgcggaattt gctgtcacgt tcaccacata aataataaaa
    28381 cgcaaatgtg cgggcgctaa ggttagcacg taaatcaggc tggacggcga gagaagtcgc
    28441 ctccagcatg gcggcaaggg ttgcgccatt attgttgctg cgcagcgcca ccagctcccg
    28501 gcgttgatcg tcattgagcg aggcaaaaac aggctgttga taccagtcgg caaataccgc
    28561 cgttaacggt tctgtgcgaa aacgctgcgc ccattggcga tcggaacgct gacgttccgc
    28621 acgttgttca gcattttgca gccccggatg cccgccttcg acaataaccc cacaaagccc
    28681 cgccagcccc tggcaagccg ccatcatcgc cacccgtccg ccaagcgagt accccaccag
    28741 ccagaagtca aggatgttgt aactaaccaa ggttttacgc agtaagtcgg tgacatcatc
    28801 aaatccatcg acgctaatcg ccgccgaacc accatgacct aggagatcaa catacaaccg
    28861 tgagtagtcg gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc
    28921 gtgcaaaaac accagccagg gtaaacctag ttttccgtgt tttgcctgcg cgtgcaggat
    28981 cataaatggc ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta
    29041 accaccattt caatcaccgt ggtggttggc gtgcgccagg cgtcggcaaa tgccgtttca
    29101 agttcctgcc agttttgcgg atgatgatat ttcagctcga acatcgcggc ggcgtgctca
    29161 aaatggacgt tttgcggcat cagatagaaa cgctcacgct cgctttgcgg cgttggcaac
    29221 agcgagaaaa tttgcccgcc gttgttgttc accacaatta ataccagcgg cgcagaaacc
    29281 tgacgcaata acgccagcgc gttgagatcg taaagcgcgg agagatcgcc cacaatcgcc
    29341 agcgtcggtt tgccgcttgc ccgctgaacg cctgcggcgg tcgaaagcag tccgtcgata
    29401 ccgctggccc ctcggttgct gtacaccggg taacctgccg gaagttgcga aagcgcatca
    29461 atcagacgta ccaccaggct gttaccaaca aacaattgcc cctgttcagg cagatagtcg
    29521 ctgatgcgat gcgccagttg cgcttcgcca aacgcatcac ggcgggcaat aaccgcctgc
    29581 attgcctgtt ccgccaggcg cgggatttca acgcaccagg gctggcgttt ttctgccgga
    29641 tgcagctcca gccagtcggc aatattggca attaagcgac gtccgcgatg gtgtgccgga
    29701 tcaagtcgcc cttcaatgtc atcaacaatc cagtactctt ctggttcaca gcttgcctgc
    29761 cattgcagga gccgtttccc cgtcaggcta cttcccagtt gcaccacaat ttgcgcctgc
    29821 tgcagctcgc tggtcgcttt ggcattgcct aaccaaagat cggcacacgg cagcggctgc
    29881 ccggtttgtg acagcacatc gccaatcagc ggccagccaa gagtttgcgc ccacagggca
    29941 acttttttgc cctcttccgc actcatgcgc ccggcaacca ccacgccgcg cttttgtcgc
    30001 cagaagaacc agtcgcgctg tttttcactt tccagacgag gcgcttcacg cagccacggt
    30061 ttgtcgtcct gccaccagtc acccagacgc tgttgccagc taagcccggt atcgtccatt
    30121 tcgccataca gcggttcagc aaacgggcag ttgatatgga ctccccccgc atgaagcgta
    30181 ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg ggtcgggcgc
    30241 ggcaatgaaa tactgtgcgt ggggtgagag gcgaacattc ccggctggcg aattgcctga
    30301 ttcgcgccgc agtcaattag ctccggcggg cgatcggcgg ttaagagaat cagtttttct
    30361 ccggttaacc cggcttcaat cagtgccgga tagagatttg ccaccgccgt gccggaggtc
    30421 acaatcaccg ccaccggctg cttgctgact ttcgccagcc ccagcgccag atgccccaac
    30481 ccacgctcat cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt
    30541 aacggtgtag aacgcgagcc tggggcgata cagatgtgtc tgacgccgtg acgcgttaat
    30601 gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc
    30661 ggtatcaata ttgcggctaa gtataaggag ctacaaaaat agattattga tatgaatcgg
    30721 taatgatgcg actcattact attccatttg taataaagta cgcagccctg ccgctttgtt
    30781 gtcgatttcc tgccactctt gctcggggtc ggaaccacgg acaatgcccg cgccagcata
    30841 taatcgcacg acattgccgc taatttttgc tgagcgcagg gaaacgcaga attcgctttg
    30901 ttgtaatgag agatagcccg cagaaccggc gtaccattct cgggtgaacg gttcgtgacg
    30961 ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca actgctgccg ttggcagcaa
    31021 ctgatgtaaa cagatcacat catccgcttt gttgagtgaa gtccagatac agcggcgaag
    31081 atgctgcact ttacgcagac gcagtacctg cggcggtaaa acatccagcg tctgggtatc
    31141 ggcctgtaat cgttggcaga tatcttccac caccagcatg ttctcgcgct ggtttttatc
    31201 atccgccatc agccactctc ctaactgctg cgcctgctta tcatcaggat gatttgctac
    31261 tgttcccgcc agcgcttcag tacgcagcgc tttgtcacgc cgccgccata accgttccgg
    31321 tgaagagcca agaaaagcat tttcgccatc aaaggccatg taaaaatggt agcaattcag
    31381 attcagtcga cgactggcag ccatcatcgc cgccgcgttg accggacttg cgaaatgcag
    31441 gtcagttgcc cgagcgagca ccactttgtc gagctcacct tcggcgatgg ttttcgttgc
    31501 cagttcgatt aattgcgtcc agcccgtttt gtccggccag tgttgttctc gcgtggtggt
    31561 taaatgtaac ccaggcaagg gcttgatact caccagtgtg gcgataaatt cttttgcctg
    31621 aatcgcatcg tgctgaaggg agctttcgct gaatagcgtc agccgcagcg tggctttacc
    31681 gccacagcgt cgccattcca ggcggggtaa aagtaaattg ccctgcgacg ggtcaaaagc
    31741 attcagcccc caaatgcgta agtcagcgtg ttccgggtgc tggcgaagaa aacgttgtgc
    31801 ctggtccaac gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt catcaccatt
    31861 acgttgttgc cagtagaatt gcgggtacgt ttgctgactg gccagccagc tcaaggcatc
    31921 aaaagcgtct ttgagaggga aaggaatatc gataacccga atgccgggtg tcgccggaat
    31981 ctcttgcgac aaatggcgca gtagattttc cagcgccgta gtaagtgatt gcacgcggac
    32041 ctctccctgt taaaagcctc acattatacg gggtactaca aaaaaaatgc agtaccccgg
    32101 tgtagggagg ttttaacggc gtgccagcaa cagtcctagt accagcccga cggccgcgcc
    32161 cacaccaatt ccttgccagg gtttttcgtg gacgtagtca tcagcacgat aaacagcctg
    32221 cttcgcccga tagtaataac tgtctgaagc ctggctaacc cgttttttca catcatccag
    32281 cgctttttct gcatgcgctt tcagctcaac atatttctga tcggcgggat cgccagagga
    32341 gcggagcacc tcttccagtg tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc
    32401 accaaactga ttagacattc tcgttctcca tttgcgtaaa acctgtcact aactatagac
    32461 aagagttgtt gattacgcct gaattacctc gcgtgccatc ccaatgtgtg ggataccatc
    32521 ttcttcatag acctccgtca cagggataaa accgaaactc tggtagaagt tttgcagatg
    32581 cgcctgcgcc ccgaggtaaa caggcttatc aggccagtga tgcgtacatg tttcgagtgt
    32641 tttactcatc agctgctgcc ccactttttc accacgcaac gcttcactga caatcacccg
    32701 acctataacg accggctcaa gatcatcatc acttttcaga atcctcgcat acgccaccag
    32761 ttcatcattt ttccaaccga gaatatggcg attatccccc gtcaaatcat caccatcgat
    32821 atcctgataa gggcaattct gctcgacaac aaacaccgcg caacgcagtt gtaataaggc
    32881 ataaagctgg gaaaccgata attcagagtg atgcagatct tgccattcaa tcataatgct
    32941 ctccttttag atgtatgcct tatactagcc ggaatatttt ttgaaacgtg atgaactcat
    33001 ggaattaatt tttttaggta cttcagccgg tgtgccaacc cgcacgcgca atgtcacggc
    33061 aatattgctt aacctgcaac atccgaccca gagcggactt tggttgtttg actgcggtga
    33121 aggcacccag catcagctac tgcataccgc ctttaaccct ggaaaactgg acaagatttt
    33181 tatcagtcac cttcatggcg atcatctttt tggtttaccc ggcttgctgt gcagtcgttc
    33241 tatgtcaggc attatccaac ccttaacgat ttatggtccg caaggtatcc gtgaatttgt
    33301 ggaaaccgcg ctgcggatta gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat
    33361 tggcgctggc gaaattctcg atgatggcct gcgcaaagta accgcttatc cgctggaaca
    33421 cccactggaa tgttatggct atcgtattga agaacatgat aaaccgggtg cattaaatgc
    33481 ccaggcatta aaagctgctg gcgtgccgcc tggcccactg tttcaggaat taaaagcggg
    33541 caaaacaatc acgctggaag atggaaggca gattaacggc gcagattact tagctgctcc
    33601 agtgccaggt aaagcgctcg ctattttcgg cgataccggc ccctgcgatg ccgcacttga
    33661 cctggctaaa ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa ccatggaagc
    33721 caaagccaat agtcgcggcc atagctctac acgccaggct gcgacactag cccgtgaggc
    33781 tggagtcggc aagctaatca ttacccacgt cagctcgcgc tatgatgaca aaggttgtca
    33841 acacctgtta cgtgaatgca ggtcaatttt cccggcgact gaactggcga atgatttcac
    33901 cgtgtttaac gtttaactct gcttattatt aaaaacaggg tgaaacttgc cctgttatag
    33961 caacccgcgc tacatatatc cacccacaca ggcatattga gcgatttttg atatttattc
    34021 atctccttac gcactatatt cagagcaata agttgcctct tttcatcttg attcagcaca
    34081 acctttgcaa ctaaaaggag cccatgatgg ttacagttgt cagcaattat tgtcaattat
    34141 ctcaaaccca actcagtcag acatttgcag aaaaatttac tgtgaccgag gaattactgc
    34201 agtctttaaa aaaaacagcg ttatccggag atgaagaaag tattgagtta ctgcataata
    34261 ttgcgttagg ttatgatgaa tttggaaaaa aagctgggta atgctgccaa cttactgatt
    34321 tagtg
//
LOCUS       DAZPFP010000027        34261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPFP010000027 DAZPFP010000000
VERSION     DAZPFP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.027"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHUR4_001233"
     CDS             complement(<1..331)
                     /locus_tag="ACHUR4_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1454888.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHUR4_001234"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHUR4_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1454889.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHUR4_001235"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHUR4_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1454890.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHUR4_001236"
     CDS             complement(1692..2072)
                     /locus_tag="ACHUR4_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR1454891.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACHUR4_001237"
     CDS             complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACHUR4_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR1454892.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACHUR4_001238"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACHUR4_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1454893.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACHUR4_001239"
     CDS             complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACHUR4_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1454894.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="ACHUR4_001240"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="ACHUR4_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1454895.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="ACHUR4_001241"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="ACHUR4_001241"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1454896.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="ACHUR4_001242"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="ACHUR4_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1454897.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="ACHUR4_001243"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="ACHUR4_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1454898.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="ACHUR4_001244"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="ACHUR4_001244"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1454899.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="ACHUR4_001245"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="ACHUR4_001245"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1454900.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="ACHUR4_001246"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="ACHUR4_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1454901.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="ACHUR4_001247"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="ACHUR4_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1454902.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="ACHUR4_001248"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="ACHUR4_001248"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1454903.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="ACHUR4_001249"
     CDS             complement(14625..15440)
                     /locus_tag="ACHUR4_001249"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1454904.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="ACHUR4_001250"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="ACHUR4_001250"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1454905.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="ACHUR4_001251"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="ACHUR4_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1454906.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="ACHUR4_001252"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="ACHUR4_001252"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1454907.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="ACHUR4_001253"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="ACHUR4_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1454908.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="ACHUR4_001254"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="ACHUR4_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1454909.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="ACHUR4_001255"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="ACHUR4_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1454910.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="ACHUR4_001256"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="ACHUR4_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1454911.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="ACHUR4_001257"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="ACHUR4_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1454912.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="ACHUR4_001258"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="ACHUR4_001258"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1454913.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="ACHUR4_001259"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="ACHUR4_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1454914.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="ACHUR4_001260"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="ACHUR4_001260"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1454915.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="ACHUR4_001261"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="ACHUR4_001261"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1454916.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="ACHUR4_001262"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="ACHUR4_001262"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1454917.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="ACHUR4_001263"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="ACHUR4_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1454918.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="ACHUR4_001264"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="ACHUR4_001264"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1454919.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="ACHUR4_001265"
     CDS             31574..32188
                     /locus_tag="ACHUR4_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1454920.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="ACHUR4_001266"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="ACHUR4_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1454921.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="ACHUR4_001267"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="ACHUR4_001267"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1454922.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8050 a         8652 c         9261 g         8298 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 catcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 tttcctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ttggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc tctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgtaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgataattt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgtatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtaat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 c
//
LOCUS       DAZPFP010000028        34130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPFP010000028 DAZPFP010000000
VERSION     DAZPFP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.028"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            41..127
                     /locus_tag="ACHUR4_001268"
                     /pseudo
     CDS             41..127
                     /locus_tag="ACHUR4_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(137..217)
                     /locus_tag="ACHUR4_001269"
                     /pseudo
     CDS             complement(137..217)
                     /locus_tag="ACHUR4_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(532..1008)
                     /locus_tag="ACHUR4_001270"
     CDS             complement(532..1008)
                     /locus_tag="ACHUR4_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1454923.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1067..2356)
                     /gene="bioA"
                     /locus_tag="ACHUR4_001271"
     CDS             complement(1067..2356)
                     /gene="bioA"
                     /locus_tag="ACHUR4_001271"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1454924.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2443..3483
                     /gene="bioB"
                     /locus_tag="ACHUR4_001272"
     CDS             2443..3483
                     /gene="bioB"
                     /locus_tag="ACHUR4_001272"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1454925.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3480..4634
                     /gene="bioF"
                     /locus_tag="ACHUR4_001273"
     CDS             3480..4634
                     /gene="bioF"
                     /locus_tag="ACHUR4_001273"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1454926.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4621..5376
                     /gene="bioC"
                     /locus_tag="ACHUR4_001274"
     CDS             4621..5376
                     /gene="bioC"
                     /locus_tag="ACHUR4_001274"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1454927.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5369..6046
                     /gene="bioD"
                     /locus_tag="ACHUR4_001275"
     CDS             5369..6046
                     /gene="bioD"
                     /locus_tag="ACHUR4_001275"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1454928.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6625..8646
                     /gene="uvrB"
                     /locus_tag="ACHUR4_001276"
     CDS             6625..8646
                     /gene="uvrB"
                     /locus_tag="ACHUR4_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1454929.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8838..9746)
                     /gene="yvcK"
                     /locus_tag="ACHUR4_001277"
     CDS             complement(8838..9746)
                     /gene="yvcK"
                     /locus_tag="ACHUR4_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1454930.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10011..10155
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10143..11132
                     /gene="moaA"
                     /locus_tag="ACHUR4_001278"
     CDS             10143..11132
                     /gene="moaA"
                     /locus_tag="ACHUR4_001278"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1454931.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11154..11666
                     /gene="moaB"
                     /locus_tag="ACHUR4_001279"
     CDS             11154..11666
                     /gene="moaB"
                     /locus_tag="ACHUR4_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1454932.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11669..12154
                     /gene="moaC"
                     /locus_tag="ACHUR4_001280"
     CDS             11669..12154
                     /gene="moaC"
                     /locus_tag="ACHUR4_001280"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1454933.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12147..12392
                     /gene="moaD"
                     /locus_tag="ACHUR4_001281"
     CDS             12147..12392
                     /gene="moaD"
                     /locus_tag="ACHUR4_001281"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1454934.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12394..12846
                     /gene="moaE"
                     /locus_tag="ACHUR4_001282"
     CDS             12394..12846
                     /gene="moaE"
                     /locus_tag="ACHUR4_001282"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1454935.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            12983..13687
                     /locus_tag="ACHUR4_001283"
     CDS             12983..13687
                     /locus_tag="ACHUR4_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1454936.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13892..14605
                     /locus_tag="ACHUR4_001284"
     CDS             13892..14605
                     /locus_tag="ACHUR4_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1454937.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(14641..15597)
                     /locus_tag="ACHUR4_001285"
     CDS             complement(14641..15597)
                     /locus_tag="ACHUR4_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR1454938.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(15597..16838)
                     /gene="clsB"
                     /locus_tag="ACHUR4_001286"
     CDS             complement(15597..16838)
                     /gene="clsB"
                     /locus_tag="ACHUR4_001286"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1454939.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(16835..17596)
                     /locus_tag="ACHUR4_001287"
     CDS             complement(16835..17596)
                     /locus_tag="ACHUR4_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1454940.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17729..18139
                     /locus_tag="ACHUR4_001288"
     CDS             17729..18139
                     /locus_tag="ACHUR4_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1454941.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18101..19207)
                     /locus_tag="ACHUR4_001289"
     CDS             complement(18101..19207)
                     /locus_tag="ACHUR4_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454942.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19218..20351)
                     /locus_tag="ACHUR4_001290"
     CDS             complement(19218..20351)
                     /locus_tag="ACHUR4_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1454943.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20344..22080)
                     /locus_tag="ACHUR4_001291"
     CDS             complement(20344..22080)
                     /locus_tag="ACHUR4_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1454944.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22073..23068)
                     /gene="hlyD"
                     /locus_tag="ACHUR4_001292"
     CDS             complement(22073..23068)
                     /gene="hlyD"
                     /locus_tag="ACHUR4_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1454945.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23071..23742)
                     /gene="cecR"
                     /locus_tag="ACHUR4_001293"
     CDS             complement(23071..23742)
                     /gene="cecR"
                     /locus_tag="ACHUR4_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1454946.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            23971..25191
                     /gene="rhlE"
                     /locus_tag="ACHUR4_001294"
     CDS             23971..25191
                     /gene="rhlE"
                     /locus_tag="ACHUR4_001294"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1454947.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(25185..25283)
                     /locus_tag="ACHUR4_001295"
                     /pseudo
     CDS             complement(25185..25283)
                     /locus_tag="ACHUR4_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25365..25847)
                     /gene="ybiA"
                     /locus_tag="ACHUR4_001296"
     CDS             complement(25365..25847)
                     /gene="ybiA"
                     /locus_tag="ACHUR4_001296"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1454948.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            25967..28117
                     /gene="dinG"
                     /locus_tag="ACHUR4_001297"
     CDS             25967..28117
                     /gene="dinG"
                     /locus_tag="ACHUR4_001297"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1454949.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28145..29107
                     /gene="ybiB"
                     /locus_tag="ACHUR4_001298"
     CDS             28145..29107
                     /gene="ybiB"
                     /locus_tag="ACHUR4_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1454950.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29248..30333
                     /gene="hcxB"
                     /locus_tag="ACHUR4_001299"
     CDS             29248..30333
                     /gene="hcxB"
                     /locus_tag="ACHUR4_001299"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1454951.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            30400..30459
                     /gene="ybiE"
                     /locus_tag="ACHUR4_001300"
     CDS             30400..30459
                     /gene="ybiE"
                     /locus_tag="ACHUR4_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1454952.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(30562..30822)
                     /gene="ybiJ"
                     /locus_tag="ACHUR4_001301"
     CDS             complement(30562..30822)
                     /gene="ybiJ"
                     /locus_tag="ACHUR4_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1454953.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31087..31353)
                     /gene="ybiI"
                     /locus_tag="ACHUR4_001302"
     CDS             complement(31087..31353)
                     /gene="ybiI"
                     /locus_tag="ACHUR4_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1454954.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(31427..32104)
                     /gene="ybiX"
                     /locus_tag="ACHUR4_001303"
     CDS             complement(31427..32104)
                     /gene="ybiX"
                     /locus_tag="ACHUR4_001303"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1454955.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32146..34130)
                     /locus_tag="ACHUR4_001304"
                     /pseudo
     CDS             complement(32146..34130)
                     /locus_tag="ACHUR4_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8057 a         8831 c         9213 g         8029 t
ORIGIN      
        1 gaggattctt taaaaaaacg cgcctgtaaa tagttaagaa caggaagaac tgttggggat
       61 gtggctggcc gaaaatgaag gggtgaagtt ctggctcaat gtgctgactg aactgaaaaa
      121 acgcggttgg tcgccctacc ttttcatcaa gccagtagtc tgcccaccac agcatcattt
      181 ctctgcgctt atcgagatac tgagcatggt tgtaaattca gctaacccag tgcgttgaca
      241 aaaaattagc gcaagaggac aaaaaatcac cttgcgctaa tgctctgtat caggtcacta
      301 ataccatcta agtggttgat tcatagtgac tggatatgtt gtgttttgca gtattatgca
      361 gtctattttt taagctaaat gtattataat gcattgatat taatgatttt tactgtttct
      421 cggtcagctt ttttatacta acttgagcga aacgggaagg taaaaagaca aaaagttgtt
      481 tttaatacct ttaagtgata ccagatggca ttgcgccatc tggcagagtg attaactaaa
      541 catcgcggta atcgatgcgc ttgccagaga gtggaaatga acgttaaacc cgaccatcgc
      601 gccgctggct ccttcatcga catcaatacg ctctacatcc agcgcgtgaa cggtaaaaat
      661 gtagcgatgg gtttcgcctt tcggcggcgc tgcgccatcg tatccggttt taccaaaatc
      721 ggtacgcgtc tgcaaaacgc cgtctggcat tgctaccaga ccagagccaa acccttgcgg
      781 taatacgcgg gtatcagcgg gtaaattaac aactacccag tgccaccagc cggagccggt
      841 tggcgcatcc gggtcgtagc aggtgacaac aaaacttttc gttcccgcag gaacatcatc
      901 ccacgccaga tgcggtgaaa tattatcgcc atcgtaaccc atgccgttaa agacatgacg
      961 atgcggcaac ttatcgccat cgcgcagatc gttactgatg agtttcatga accctccttt
     1021 cttgtttgca gaaagtgtag ccagaaaccc tcacgctgac ttcccgttat tggcaaaaaa
     1081 atgtttcatc ctgtaccgcg cggttaaccg ctgcggtcag acgctgcaac tgttgcggga
     1141 gaataatata gggcggcatc aggtaaatca gtttgccaaa aggccggatc cataccccct
     1201 gttcgacaaa gaatttttgc agcgccgcca tattcaccgg acgagtggtt tcgaccacac
     1261 cgattgcccc cagtacgcgc acatcggcaa ccatttcggc atcacgggct ggtgccagtt
     1321 gctcgcgcag ctgcacttca atagccgcca cctgctgctg ccattcgccg gattcgataa
     1381 tcgctaggct ggcgtttgct gccgcgcagg ccagcggatt gcccataaaa gttggcccat
     1441 gcataaagca gccggcttcg ccgttactga tggtttctgc aacctcgcgc gtggtaagtg
     1501 tggcggaaag ggtcattgtg ccgccggtta aggctttacc gaggcacaaa atgtccggcg
     1561 cgatttctgc atattcacag gcaaacagtt tgccggtacg accaaatccg gtggcgatct
     1621 cgtcggcaat cagcaagata ccttcgcgat cgcacatttt gcggattcgt tttaaccatt
     1681 ccggatggta catgcgcatc ccgcctgcgc cctggacaat cggctcaatg atcaccgctg
     1741 cgatttcatg acgatgcgcc gccatcaggc gggcaaaacc aaccatatcg cgttcatccc
     1801 attcaccatc catgcggctt tgcggggcgg gagcaaacaa gttttctggc agatagcctt
     1861 tccacaggct gtgcattgag ttgtccggat cgcacaccga catcgcgcca aaggtatcgc
     1921 catgataacc attgcggaag gtcagaaaac gctggcgcgc ttcgcctttg gcttgccagt
     1981 actgcaacgc cattttcatc gccacttcca ccgctacgga accggagtcc gcgagaaaaa
     2041 cgcactccag cggttgcggc gtcattgcca ccagtttgcg gcatagctcg atggcgggcg
     2101 catgggtgat accgccaaac atcacatgcg acatggcatc aatttgcgtc ttcatcgccg
     2161 cattaagctg cgggtgattg tagccgtgga tcgccgccca ccaggacgac ataccgtcaa
     2221 ccaggcgtct gccgtcagac aaaattagct cgcaaccttc ggcgctcgcc accggataaa
     2281 ccggcagagg ggaggtcatg gatgtgtatg ggtgccagat atggcgttgg tcaaaggcaa
     2341 gatcgtccgt tgtcataatc gacttgtaaa ccaaattgaa aagatttagg tttacaagcc
     2401 tacaccgaat taacaacaaa aaacacgttt tggagaagcc ccatggctca ccgcccacgc
     2461 tggacattgt cgcaagtcac agaattattt gaaaaaccgt tgctggatct gctgtttgaa
     2521 gcgcagcagg tgcatcgcca gcatttcgaa cctcgtcagg tgcaggtcag cacgttgctg
     2581 tcgattaaga ccggagcttg tccggaagat tgcaaatact gcccgcaaag ctcgcgctac
     2641 aaaaccgggc tggaagccga gcggttgatg gaagttgaac aggtgctgga gtcggcgcgc
     2701 aaagcgaaag cggcaggatc gacgcgcttc tgtatgggcg cggcatggaa gaatccccac
     2761 gaacgcgata tgccgtacct ggaacaaatg gtgcaggggg taaaagcgat ggggctggag
     2821 gcgtgtatga cgctgggcac gttgagtgaa tctcaggcgc agcgcctcgc gaacgccggg
     2881 ctggattact acaaccacaa cctggacacc tcgccggagt tttacggcaa tatcatcacc
     2941 acacgcactt atcaggaacg cctcgatacg ctggaaaaag tgcgcgatgc cgggatcaaa
     3001 gtctgctcag gtggcattgt gggcttaggc gaaacggtaa aagatcgcgc cggattattg
     3061 ctgcaactgg caaacctgcc gacgccgccg gaaagcgtac caatcaacat gctggtgaag
     3121 gtgaaaggca cgccgcttgc cgataacgat gacgttgatg cctttgattt tattcgcacc
     3181 attgccgtag cgcggatcat gatgccgacc tcttacgtgc gcctttctgc cggacgcgag
     3241 cagatgaacg aacagactca ggcgatgtgc tttatggcag gcgcaaactc gattttctac
     3301 ggttgcaaac tgctgaccac gccgaatccg gaagaagata aagacctgca actgttccgc
     3361 aaactggggc taaatccgca gcaaactgcc gtgctggcag gggataacga acaacagcaa
     3421 cgtcttggac aggcgctgat gaccccggac accgacgaat attacaacgc ggcagcatta
     3481 tgagctggca ggagaaaatc aacgcggcgc tcgatgcgcg gcgtgctgcc gatgccctgc
     3541 gtcgccgtta tccggtggcg caaggagccg gacgctggct ggtggcggat gatcgccagt
     3601 atctgaactt ttccagtaac gattatttag gtttaagcca tcatccgcaa attatccgtg
     3661 cctggaagca gggggcggag caatttggca tcggtagcgg cggctccggt cacgtcagcg
     3721 gttatagcgt ggtgcatcag gcgctggaag aagaactggc cgagtggctt ggctattcgc
     3781 gggcgctgct gtttatctct ggttttgccg cgaatcaggc agtcattgcc gcgatgatgg
     3841 cgaaagagga ccgtattgtt gccgaccggc ttagccatgc ctcattgctg gaggctgcca
     3901 gtttaagccc gtcgcagctt cgccgtttta ctcataacga tgtcgctcat ctggcgcgac
     3961 tgcttgcttc cccctgtccg gggcagcaac tggtggtgac agaaggcgtg ttcagcatgg
     4021 acggcgatag cgcgccactg gcggaaatcc agcaggtaac gcaacagcac aatggctggt
     4081 tgatggtcga tgatgcccac ggcacgggcg ttatcgggga gcaggggcgc ggcagctgct
     4141 ggctgcaaaa ggtaaaacca gaattgctgg tagtgacttt tggcaaagga tttggcgtca
     4201 gtggggctgc ggtgctttgc tcaagtacgg tggcggatta tctgctgcaa ttcgcccgcc
     4261 accttatcta cagcaccagt atgccgcccg ctcaggcgca ggcattacgt gcgtcgctgg
     4321 cggtcattcg ccgtgatgag ggggatgcac ggcgcgaaaa actggtgtca ctcattgcgc
     4381 gttttcgtgc cggagtacag gatttgccgt ttacgctggc tgattcatgc agcgccatcc
     4441 agccattgat cgtcggtgat aacagccgtg cgttacaact ggcagaaaaa ctgcgccagc
     4501 aaggctgctg ggtcacggcg attcgcccgc caaccgtacc cgctggtact gcgcgactgc
     4561 gcttaacgct aaccgctgcg catgaaatgc aggatatcga ccgtctgctg gaggtgctgc
     4621 atggcaacgg ttaataaaca agccattgca gcggcatttg gtcgggcagc cgcacactat
     4681 gagcaacatg cagatctaca gcgccagagt gctgacgcct tactggcaat gcttccacag
     4741 cgtaaataca cccacgtact ggacgcgggt tgtggacctg gctggatgag ccgccactgg
     4801 cgggaacgtc acgcgcaggt gacggcgtta gatctctcgc cgccaatgct tgcgcaagcc
     4861 cgccataaag atgccgcaga tcattatctg gcaggagata tcgaatccct gccgttagcg
     4921 actgcgacgt tcgatcttgc atggagcaat ctcgcggtgc agtggtgcgg taatttatcc
     4981 acggcactcc gcgagctgta tcgggtggtg cgccccggag gcgtggtcgc gtttaccacg
     5041 ctggtgcagg gatcgttacc cgaactgcat caggcgcggc aggcggtgga cgagcgtccg
     5101 catgctaatc gctttttacc gccagatgaa atcgaagagt cgctgaacgg cgtgcgttat
     5161 caacatcata ttcagctcat cacactatgg tttgatgatg cgctcagtgc catgcgttcg
     5221 ctgaaaggca tcggtgctac gcatcttcat gaagggcgcg acccgcgaat attaacgcgt
     5281 tcgcagttgc agcgattgca actggcctgg ccgcaacaac aggggcgata tcctctgacg
     5341 tatcatcttt ttttgggagt gattgctcgt gagtaaacgt tattttgtca ccggaacgga
     5401 taccgaagtg gggaaaaccg tcgccagttg tgcactttta caagccgcga aggcagtagg
     5461 ttatcggacg gcaggttata aaccggtcgc ctctggcagc gaaaagaccc cggaaggctt
     5521 acgtaatagt gacgcgctgg cgttacagca caacagcagc ctgcagctgg attacgcaac
     5581 ggtaaatcct tacaccttcg cagaatccac ctcgccgcac atcatcagcg cccaagaggg
     5641 caggccgata gaatcattgg taatgagcgc cggattacgc gcgcttgaac aacaggctga
     5701 ctgggtgtta gtggaaggtg ctggcggctg gtttacgccg ctttctgaca ctttcacttt
     5761 tgcagattgg gtaacacagg aacaactgcc ggtgatactg gtagttggtg tgaaactcgg
     5821 ctgtattaat cacgcgatgt tgactgcgca ggcaatacaa cacgccggac tgactctggc
     5881 gggttgggtg gcgaacgatg ttacgcctcc gggaaaacgt cacgctgaat atatgaccac
     5941 gctcacccgc atgattcccg cgccgctgct gggagagatc ccctggcttg cagaaaatcc
     6001 agaaaatgcg gcaaccggaa agtacataaa ccttgccttg ttgtagccat tctgtatttg
     6061 gttaaattgc gagcgagatc gcgtcttcga ttgactgcaa tttaaccaat taaattctaa
     6121 aataatcacg aaaaaaattt tacttccgcc tcatgcggcg aatgtgggaa ttgcccaggc
     6181 ggcgggggat aggggctgga gacagttatc cactattcct gtggataacc atgtgtatta
     6241 gagttagaaa acacgaggca agcgagagaa tacgcggctt gcacgcgaat tggcgttaaa
     6301 gacggctcaa agaaatatct tttatttttt aactggttag ataaatgcaa tggcagtcac
     6361 tgaacaggca tctcttgcca taaaactgtc atcactcatc ttgacaaatg ttaaaaaagc
     6421 cgttgctttg gggataaccc ggtaaggctg gagttttatc tcgccacaga gtaaattttg
     6481 ctcatgattg acagcggagt ttacgctgta tcagaaatat tatggtgatg aactgttttt
     6541 ttatccagta taatttgttg gcataattaa gtacgacgag taaaattaca tacctgcccg
     6601 cccaactcct tcaggtagcg actcatgagt aaaccgttca aactgaattc cgcttttaaa
     6661 ccttctggcg atcagccaga ggcgattcga cgtctcgaag aggggctgga agatggcctg
     6721 gcgcaccaga cgttacttgg cgtgactggc tccgggaaaa ccttcaccat tgccaatgtc
     6781 attgctgacc ttcagcgccc aaccatggta cttgcgccca acaaaacgct ggcggcccag
     6841 ctgtatggcg aaatgaaaga gttcttcccg gaaaacgcgg tggagtattt cgtctcctac
     6901 tacgactact atcagccgga agcctatgta ccgagttccg acaccttcat tgagaaagat
     6961 gcctcggtta acgaacatat tgagcagatg cgtttgtccg ccaccaaagc gatgctggag
     7021 cggcgtgatg tgattgtagt ggcgtctgtt tccgcgattt atggtctggg cgatcctgat
     7081 ttatatctca agatgatgct ccatctcacg gtcggtatga ttatcgatca gcgcgcgatt
     7141 ctgcgccgac tggcggagct gcaatacgct cgtaatgatc aagcattcca gcgtggtact
     7201 ttccgcgttc gtggcgaggt gatagatatc ttcccggcag aatcggatga cattgcactt
     7261 cgcgtggaac tgtttgacga ggaagtggaa cgattgtcgt tatttgaccc gctgaccggg
     7321 cagattgttt ccactattcc acgttttacc atctacccga aaacgcacta cgtcacaccg
     7381 cgcgagcgca tcgtacaggc gatggaggag atcaaagaag aactggccgc cagacgcaaa
     7441 gtgctgttgg aaaacaacaa actgctggaa gagcagcggc tgacccagcg tacccagttt
     7501 gatctggaga tgatggacga gctgggctac tgttcgggga ttgaaaacta ctcgcgcttc
     7561 ctctccggtc gtggaccggg tgagccaccg ccgacgctgt ttgattacct gcctgccgat
     7621 gggctgctgg tcgtcgatga atctcacgtc accattccac aaattggcgg catgtatcgc
     7681 ggtgaccggg cgcgtaaaga gacactggtg gagtacggct tccgcctgcc atcagcgctg
     7741 gataaccgtc cgcttaagtt tgaagagttc gaagcattag cgccgcaaac catctatgtt
     7801 tcggcgacgc cgggtaatta cgagctggaa aaatccggcg gcgatgtggt ggatcaggtg
     7861 gtgcgtccaa ccggattgct tgacccgatt atcgaagtgc ggccggtggc gacacaggtt
     7921 gatgatcttc tttcggagat tcgtcagcga gcggcaatta acgaacgcgt gctggtcacc
     7981 acactgacca agcgaatggc ggaagatctt accgaatatc tcgaagaaca cggtgagcgc
     8041 gtgcgttatc ttcactcaga tatcgacacc gtcgagcgta tggagattat ccgcgacttg
     8101 cgtctgggtg agttcgacgt attggtaggg atcaacttac tgcgtgaagg tctggatatg
     8161 ccggaagtgt cgctggtggc gatcctcgac gctgacaaag aaggcttcct gcgttccgaa
     8221 cgttcgttga tccagaccat tggtcgtgcg gcacgtaacg ttaacggtaa agcgattctc
     8281 tacggcgata ggagcactcc atcaatggcg aaagcgattg gcgaaaccga acgtcgccgt
     8341 gagaaacagc agaagtacaa cgaggaacac ggcattacgc cgcaaggctt gaacaagaaa
     8401 gtggtcgata tcctggcgct ggggcagaac attgccaaaa ccaaagcgaa gggcagagga
     8461 aaatcgcgcc cgattgttga gccggataat gtgccgatgg atatgttgcc gaaagcgttg
     8521 cagcagaaaa tccatgaact ggaagggctg atgatgcaac acgcgcagaa tctggagttc
     8581 gaagaagcgg cacaaattcg tgaccagttg catcagttgc gtgagttgtt tattgccgca
     8641 tcgtaacagg atagcgaaga agactgatga caaacggaaa acagtctgat gcgctacgct
     8701 tatcaggcct acattttctc cgcaatatat tgaatttgcg cggtttgtag gccggataag
     8761 gcgttcacgc cgcatccggc ataaacaacg cgcactttgt cttcaatagg aagccggaat
     8821 tttccttcgg atttccgtta acctaaagcc tgtaacgcct tttccagcgc gttatgtaac
     8881 aactggcggt catgacgata cggaatatcg ctggcctcca gtacctcctg gatcacaatc
     8941 cgctctttca ccgccgagac atccactttt ggcccgacga tgaccgcatc aatgaccttt
     9001 ttaccaacat actgctccat aattgccagc ttgctttcca gcttcaaatt agccgcaggt
     9061 aaactcaact cacgccccag attgccgata taaaccatcg gcgctggcgt gcggcgtaat
     9121 gcctgggcga tttccttcag cagcagaatt ggcatcaggc tggtataaaa actgccaggc
     9181 ccaataatga tgagatccgc ttcattgata gcgtgaaccg cctcacgcgt tgcgggtaca
     9241 ttaggcgtta acaataactc ttgaatcggc gtagttaact ggtcgatatt gacctcgccg
     9301 taaacttcat gcccctgatc gtcaatcgcc atcagatcaa caggatgctc tgacattgga
     9361 atcaaatgcg tatccacttt cagcagatta cgaattaaat tgatggcttc cagaggccgc
     9421 acgctaaggt gatccagcgc ctttaacatc aagtttccga gattatgacc ggaaagttcg
     9481 ccattgccgc caaaacggta ttcaaacatc gcggaggcga cgttcggttc cgttatcagc
     9541 tggttgaggc agttgcgcat atcgccccaa gcaatgccgc cttctgaacg gcgaatacgc
     9601 cccgtcgagc caccattatc cgtggtggtg acgatacccg ttaaacgaga acccaaagac
     9661 gaaagtgatg agagaacgcg tcccagtcca tgccctccgc cgagagcaac gacacgatca
     9721 agatcagcca gcgtacgatt gcgcatatag tttcctggag tcagattatc cgcgctacag
     9781 tagcgcaaat tgcggggaaa cagcaattaa cctgccaata ttgaggatac aaactatttt
     9841 cttctatctc taaaggacga tgcacgctat gcctccctga tgatgtatat caaagtaaaa
     9901 ccgccatttt cccttattct gtagcgaaat agcacgatca tgacgctata tacatgatta
     9961 catagcgaaa gtgtggatgg taaaaatctc atttacacgc tagtatcggc ttaaccacta
    10021 aacactctag cctctgcacc tgggtcaact gatacggtgc tttggccgtg acaatgctcg
    10081 taaagattgc caccagggcg aaggaagaaa tgacttcgcc tcccgtatct ggaaaggtgt
    10141 acatggcttc acaactgact gatgtatttg cgcgtaagtt ttactacttg cgcctgtcga
    10201 ttaccgatgt gtgtaacttt cgttgcacct actgcctgcc ggatggctac aaaccgagcg
    10261 gcgtcaccaa taaaggcttt cttaccgtcg atgaaattcg ccgggttacg cgcgccttcg
    10321 ccagtctggg caccgaaaaa gtgcgcctga caggaggaga gccgtcttta cgccgtgact
    10381 ttaccgatat catcgccgct gtgcgggaaa acgacgctat ccgccagatt gcggtcacca
    10441 ccaatggtta ccgtctggaa cgcgatgtgg cgaactggcg cgatgcggga cttactggca
    10501 ttaacgtcag tgtcgacagt ttggacgccc gccagtttca cgctattacc gggcaggata
    10561 aattcaacca ggtcatggca gggattgatg ctgcatttga ggccggtttt gagaaggtca
    10621 aagtcaatac cgtgctgatg cgtgatgtta atcatcacca gctcgacacc tttctgaact
    10681 ggatccagca tcgccctatc cagctgcgtt tcatcgaact gatggaaacg ggcgagggca
    10741 gcgagctctt ccgtaagcat cacatctctg gtcaggttct gcgtgacgag ctactgcgtc
    10801 gcggctggat ccaccaatta cgtcaacgca gcgacggtcc cgcgcaagtc ttttgccatc
    10861 cagattacgc cggagagatt ggccttatca tgccgtatga aaaagacttc tgcgccactt
    10921 gcaaccgcct gcgcgtttcc tccattggta aactccatct ctgcctgttt ggtgaaggcg
    10981 gcgttaacct gcgcgatctg ctggaaggcg atacccagca acaggcgctg gaagcgcgta
    11041 tttcagcggc gctgcgggag aagaaacaga cccatttcct gcatcaaaac aacaccggta
    11101 ttacgcaaaa cttatcgtac attggcggct aaaacgtcaa aaggagagat cagatgagtc
    11161 aggtaagcac tgaatttatc ccgacccgta ttgctattct tacggtttct aatcgtcgcg
    11221 gtgaagaaga cgatacctcc ggtcactatc tgcgcgattc ggcgcaagaa gcgggccatc
    11281 acgttgtcga tgaagccatt gtgaaagaaa accgctacgc tattcgcgct caggtatctg
    11341 cgtggatcgc cagcgacgat gtacaagtgg tattgattac gggtggtact ggcctgacgg
    11401 aaggtgatca ggctcccgaa gcgctgctgc cgttgttcga ccgtgaggtt gaaggttttg
    11461 gtgaagtgtt ccgtatgttg tcgtttgaag agattggcac ttccacgttg caatctcgtg
    11521 cggtagcggg cgtcgctaac aaaacgctga ttttcgccat gccgggttcg accaaagcgt
    11581 gccgtaccgc atgggaaaat atcatcgcgc cgcagctgga tgcccgtacg cgtccgtgta
    11641 atttccatcc acatttgaag aaataagtat gtcgcaactg acccatatca acgccgctgg
    11701 cgaagcgcac atggtggatg tctccgccaa agcggaaacc gtgcgtgaag cgcgggcgga
    11761 agcctttgtc accatgcgca gcgagacgct ggcgatgatt attgatggtc gccaccacaa
    11821 aggcgacgta tttgccactg cgcgtattgc cggtattcag gcggcaaaac gcacttggga
    11881 tctgatcccg ctgtgtcacc cgctgatgct cagcaaagtt gaagtcaatt tacaggccga
    11941 gccggagcac aatcgggtgc gtatagaaac cttatgccgc ctgaccggga aaaccggtgt
    12001 cgaaatggaa gcgttaaccg cggcctccgt ggcggcgctg accatttatg acatgtgcaa
    12061 agcggtgcaa aaagatatgg tgattggtcc ggtacgtttg ctggcgaaga gcggcggcaa
    12121 gtcgggtgac tttaaggtgg aagcggatga ttaaagttct ttttttcgcc caggtgcgcg
    12181 agttggtggg aacagatgca accgaagtgg ctgcggatta cccaaccgtt gaagcgttac
    12241 gccagcacct ggctgcgcag agcgatcgct gggcgctggc gctggaagat ggcaaattgc
    12301 tggctgccgt caaccagacg ctggtgagtt ttgaccatcc gctgactgac ggcgacgaag
    12361 tagctttctt cccgccggta accggaggtt aagatggcag aaaccaaaat tgttgtcggc
    12421 ccgcagccgt tcagcgtagg agaagagtac ccgtggctgg cggagcgtga cgaagacggt
    12481 gcggtagtca ccttcaccgg aaaggtgcgc aaccataacc tgggcgacag cgtcaaagca
    12541 ttaaccctcg aacactatcc ggggatgact gaaaaagcac tggcagaaat tgttgatgaa
    12601 gcgcgtaacc gctggccgct ggggcgcgtc acggtgattc accgcatcgg ggaattatgg
    12661 ccgggcgatg aaatcgtttt tgtcggtgtc accagtgcgc atcgcagcag tgcgtttgaa
    12721 gccgggcagt ttattatgga ttatctcaaa acccgcgcac cgttctggaa gcgcgaagcc
    12781 acgccggaag gcgaccgctg ggttgaagcc cgggagagcg atctgcaggc ggcaaaacgc
    12841 tggtagtttt ttgttagccg gataaggcac cgctgcgtcc ggcaatttca ccgctcagta
    12901 catgcgttta cgctgcgtcg atgcacagcc tcatcatttt gcagtatcct taagatattc
    12961 cttatatctt caggagatcg tcatggacag attcccacgt tctgattcaa tcgtacaacc
    13021 ctgggctggc ttgcaaactt atatggcgca agtctatggc tggatgaccg ttggcttgtt
    13081 gctgaccgca tttgttgcct ggtatgcggc taattccgcg gccgtgatgg agctgttgtt
    13141 cactaaccgt gtctttttaa tcggtctgat catcgcgcaa ttagcattgg ttattgtgtt
    13201 atcagcgatg attcaaaagc tgagcgcagg tgtaacgacg atgctcttta tgctttattc
    13261 ggcgctgacg ggtcttacgc tttccagtat attcattgtc tataccgctg cttctatcgc
    13321 cagtactttc gtcgttactg ccgggatgtt cggcgcaatg agcctgtacg gttacaccac
    13381 gaagcgcgat ttaagtggct ttggcaatat gctgtttatg gcgttaatcg gcattgtgct
    13441 ggcatcgctg gtcaacttct ggctgaaaag cgaagcattg atgtgggcag ttacctacat
    13501 cggcgtgatt gtctttgtcg gattgacggc gtatgacact cagaaattga aaaatatggg
    13561 tgagcagatt gatacccgcg acacgtcgaa cctgcgcaaa tattccattc ttggtgcgtt
    13621 aaccttgtat ctggacttca tcaacctgtt cctgatgttg ttacggatct tcggcaaccg
    13681 ccgttaattc tttttcacca tcactatccc ggccatgtgt cgggattttt ttcttactaa
    13741 aaacgacact taacaagtac caggtataaa atatgcctta tgagcgagcg gaatcgtttt
    13801 ccgtatccat aaatacaaaa gtattttgta tgcgtggttt taaatttatt gatggtgaat
    13861 attaatgttt tttctaagga caactaaaag aatggaatca tactcgcaaa acagcaataa
    13921 attagatttt cagcacgagg ccaggatatt aaacggtata tggctcatta ccgctttagg
    13981 tttggtggca accgcaggac tagcctgggg agctaagtat ctcgaaatta cggcaaccaa
    14041 atatgattca ccaccaatgt atgtcgccat agggttatta ttactttgta tgtatggctt
    14101 aagtaaggat atcaacaaga taaatgccgc catcgcgggc gtaatatatc tgtttttact
    14161 ctctttggtg gcgattgtcg ttgcaagttt agttcctgta tatgccatta tcatcgtgtt
    14221 cagcactgcg ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga
    14281 tcctggttct caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat
    14341 cgtgaatgcg gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt
    14401 gttatggcca ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg
    14461 ccatagtgaa gagttgtgga gtccggttcg ttgcgctttt acaggtgcat taacactcta
    14521 ttactatttt atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca
    14581 aaggcatacg cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact
    14641 tcacatcgcc gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta
    14701 acagatcagc gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc
    14761 ggcgataatg ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc
    14821 cagcacaccg atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa
    14881 cacgcccagt acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc
    14941 cattaccatc cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg
    15001 cagcaccagc ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca
    15061 caaataaacc gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc
    15121 gacataccag taatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa
    15181 aatatagccc agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc
    15241 cggtaaccct agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact
    15301 gagcgtcagg ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag
    15361 tttgtgaccg cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac
    15421 gaccaccagc ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca
    15481 gacctcttcc cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa
    15541 aaacagccag gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag
    15601 gggtttaccc ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg
    15661 gggggatcaa cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg
    15721 aagtggcgta aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg
    15781 ggcagcatgg tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg
    15841 cgcagcgtct ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac
    15901 aaactgagcg gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc
    15961 actttgccgt ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga
    16021 tagttataca gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg
    16081 atcagtttga tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag
    16141 ccggggaaga agtaggcgtt ggcgataatc acttcccgct gcgcctgagt gagcattttc
    16201 agataatggc gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc
    16261 tgcgcttctc ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt
    16321 gccgcgctct gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc
    16381 ccttcaaggc gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg
    16441 gcggagtaat tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga
    16501 tgcatccggc gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag
    16561 cggaacacta cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa
    16621 ccgtagccat ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc
    16681 gcatgcagct gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt
    16741 tcttgtgcct cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc
    16801 aactggatct tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag
    16861 gggcatgatc agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc
    16921 tggcattttt gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc
    16981 gcgccgggcg tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg
    17041 gatgattagc tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt
    17101 ccggtagctc attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac
    17161 gcaggcccag atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc
    17221 ggcagtagag cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat
    17281 gttcaatggg ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat
    17341 tgcgaccgta ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat
    17401 cgggccagtt ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc
    17461 aaacaatatc ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc
    17521 ggttaaacgc ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt
    17581 gtgtttgatc gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc
    17641 ggcgtcacaa aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag
    17701 tagagataaa ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac
    17761 gggtctaagt tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg
    17821 ctggatgagt aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac
    17881 ggtagtgatg atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga
    17941 cgtgctggaa gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct
    18001 gttgtcccgc gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc
    18061 tggcccggcg gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa
    18121 acatcgcgta cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat
    18181 tccacacaat atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt
    18241 ggcgaatagg gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg
    18301 aaacgtaacc ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct
    18361 gctgttgtgt cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt
    18421 aaatcaccat cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc
    18481 agataccaat cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg
    18541 ctttgccgat gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac
    18601 cttgttcacg ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga
    18661 tcatggcgat cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt
    18721 tgcgtaccac cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat
    18781 aatttttgac gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc
    18841 cgtcgaggat caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag
    18901 ccgggaaacg caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg
    18961 ggcttttcag cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt
    19021 ccaccgaatg ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca
    19081 gcgtggcggc gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc
    19141 gggtttgtgg ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat
    19201 gaaacatgct cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata
    19261 aacatcaccg ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc
    19321 ccggcgagga acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc
    19381 accgcgcgga tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc
    19441 gacggcagaa aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc
    19501 gtggaaatca gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac
    19561 agaatcagca gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac
    19621 agcaacatcg ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc
    19681 gtgcgggtaa tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc
    19741 accaccagcg aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg
    19801 ataataaagt gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata
    19861 agcggttcaa aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc
    19921 ccttcgacat acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc
    19981 tgaatcggtg cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc
    20041 accagaccgc gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg
    20101 atggtggcgt cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg
    20161 ctacgctgtt ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa
    20221 ccaaaaataa acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg
    20281 cgaacgatct gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc
    20341 gggttactca ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc
    20401 catggtggga tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc
    20461 gctggcgatt aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc
    20521 cataaagtgg gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag
    20581 ccaaaattca cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat
    20641 atccggttca tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag
    20701 ttcatcggtg gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat
    20761 tttttcgttc tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg
    20821 ttcgaccgtc aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg
    20881 cgctttaccg gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg
    20941 caccagcaaa ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag
    21001 caaaccaaaa atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc
    21061 accaaatttc ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac
    21121 cgtatgcaat attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat
    21181 aaacgcatct tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc
    21241 atggcgaata tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg
    21301 aatcatgcca tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc
    21361 ctcgtgtgga ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg
    21421 ttctccctga tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc
    21481 ttcgtcgaga tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac
    21541 catctgccac agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag
    21601 caacactttc ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc
    21661 ggagagcttg cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg
    21721 agcaaaagtt tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag
    21781 gttctccatc accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac
    21841 ggcgtgcagc gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct
    21901 gtcgggtttc agtaatcccg ccaacatccg catcagtgtg gttttacctg caccgtccgg
    21961 ccccaccaac cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg
    22021 cttgtccatg cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat
    22081 gtcctgcctc gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg
    22141 cgtcggtcac cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga
    22201 cggttttcgg ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct
    22261 tgtccgggcg accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat
    22321 tccgttcatc aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg
    22381 tgccaccttc attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag
    22441 acggtgctat caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg
    22501 cctgttcgag gctggctttc gcctgagcga tgtcctgttc acggttaccg gaacggtact
    22561 gacgcaattt atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg
    22621 cattttccag gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat
    22681 agaagttctg cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag
    22741 cgatttcttc atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga
    22801 cgcccgcttt cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca
    22861 cctggcccgc tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa
    22921 cacggaaact aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat
    22981 cctggcggct ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg
    23041 ccagtccgat cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa
    23101 aatcaggtcg atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa
    23161 cgcggtccag ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc
    23221 accaatcaac gcatgggtat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca
    23281 ggcggcaatc aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag
    23341 gtggtaggct gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt
    23401 gtcatcctgg gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat
    23461 ggcagcccga tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg
    23521 gaactgctcg ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt
    23581 cgaaccgaag tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg
    23641 ggtggtggcg ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt
    23701 tttcgcctgt tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa
    23761 tcaaatgatt gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt
    23821 tagctacgcc agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc
    23881 tccctcatga cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac
    23941 cggtaacggt cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct
    24001 gatatcctgc gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag
    24061 gcgatccctg ctgtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc
    24121 aaaacagcgg gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc
    24181 aaagggcgtc gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag
    24241 attggcgaaa acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt
    24301 ggcggtgtca gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg
    24361 gcaacgccgg gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt
    24421 gaaatcctcg tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc
    24481 cgtcgcgtgt taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc
    24541 tctgacgata ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg
    24601 gcacgccgca ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa
    24661 cgcaaacgcg aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg
    24721 tttacccgta ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc
    24781 cgtagtgtgg cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat
    24841 tttaaatcgg gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat
    24901 atcgaagagt tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc
    24961 caccgtatcg ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt
    25021 gttgatgaac acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc
    25081 attgcgattc cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt
    25141 cgccagcaac gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg
    25201 cttatcaggc ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat
    25261 aaggcgttta cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac
    25321 gggaaaaaat ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc
    25381 aattgttcgc gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca
    25441 ccgtctcccc agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc
    25501 agcaagagca cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc
    25561 acctgttctt taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg
    25621 cgagcggcga ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg
    25681 aatttttgtg cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg
    25741 ggcctggcgg caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg
    25801 atggtgtctt gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat
    25861 gttggtggtt attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga
    25921 aaaatgccac aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc
    25981 cgcgcttaaa gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt
    26041 tattccccgt gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga
    26101 agaagggcgg catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt
    26161 gatccccggc atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa
    26221 cgtggcgttg caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc
    26281 cgatcttaaa ttcactgccg ctttcgggcg tgggcgctac gtttgtccac gtaatctgac
    26341 ggcgctcgcc agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact
    26401 gacgccaaat aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac
    26461 ttataaatgg gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg
    26521 tcgtttgagt accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg
    26581 cccgtttttt gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc
    26641 gctggtgatg gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct
    26701 ggtgctggac gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc
    26761 tgaaatcacc gtgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac
    26821 ctgcatggag cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa
    26881 tgcgcattgt gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta
    26941 catgcctgcc gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt
    27001 gctggagatc tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt
    27061 atttcttaac gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt
    27121 gattttacag atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct
    27181 ggcgtctctg gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg
    27241 cgaagggcag ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga
    27301 aaggctgctg tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct
    27361 gaatagtttt tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt
    27421 tgtggcgctg gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat
    27481 gcgctttgag ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt
    27541 ccgtgaacag gtggagagca aaaaatatct cggtatgttg gtactgtttg ccagcggacg
    27601 ggcgatgcag cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg
    27661 cgatcagccg cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg
    27721 tagcgtgctg gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct
    27781 cagccaggtg catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac
    27841 cgaaggggaa tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag
    27901 cgcctcgttt aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg
    27961 cgaagtggtt atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga
    28021 tgcattaccg gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa
    28081 agaaaaaacg aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc
    28141 gtaaatggac tatcgcaaaa tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga
    28201 tttagaccgg gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga
    28261 ccttgagttg ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat
    28321 gctcggcttt tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa
    28381 gccgatgccg attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc
    28441 attattggcg attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga
    28501 agatccaact cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt
    28561 gcacggcggg caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg
    28621 cgcgttttgc ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa
    28681 cagtgcacat accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct
    28741 ttccagcgtt tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg
    28801 cgggtgggcg ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg
    28861 cccgcagatc aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc
    28921 tgctggtagc gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga
    28981 gcgttgcctg gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg
    29041 cctggtggct acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc
    29101 attttaattt ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata
    29161 tgaaattcct gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca
    29221 aacacataac ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg
    29281 ctgcacagtt ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa
    29341 ttagttgccg atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc
    29401 atgatcccaa gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc
    29461 aaaatcgtta aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag
    29521 gtcgcggcac acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct
    29581 gccgtggcgc tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt
    29641 tcagcggcgg ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg
    29701 ccgttccacg gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt
    29761 aaagataatt tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc
    29821 cgcgtcgcct ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc
    29881 gtgccgacga ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt
    29941 gccgaacata aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct
    30001 ggcggtaaaa cgacgcatca ggaaacgttg caaaccagcc ccgatgccat tcttaactgc
    30061 atgaccacta tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc
    30121 gaagcctttg ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta
    30181 ccgggcgagt gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat
    30241 gcgggaagct ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg
    30301 ttgcaggctt tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg
    30361 caaacaaggg taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga
    30421 cagggaaata cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt
    30481 tgtaggcctg ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa
    30541 acgccttatc cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt
    30601 gttggtggcg gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag
    30661 tttcgcttcc agggcatcga ggggggatgc gccatcggca gaaaccacgc cgattttatt
    30721 catgttctgt gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt
    30781 tgaaagagcc atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt
    30841 gatttttgta taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga
    30901 gataaaatcg aagagaattg accgccttgt tcaaataaat tgattgatat ctaacatact
    30961 gataaaccat ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca
    31021 gcgttaaagg cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag
    31081 ttgtagttaa cgtaactgtc tgtctttcga acctctgcga ttatatcctg tatacgctgg
    31141 tttttgtaaa tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc
    31201 ccgacgggct tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg
    31261 cggaatttca ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt
    31321 gacggcgtca tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta
    31381 cagttaagtg tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc
    31441 attcccgcag cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat
    31501 agcggctttt cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat
    31561 catcgcggat catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg
    31621 gtgtcacgca atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta
    31681 cccgatgttg tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat
    31741 cgcttaaaaa cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc
    31801 gtactgcgcc atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg
    31861 gcgtggaaag ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt
    31921 tcagcacctc attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt
    31981 tcttaacttg tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt
    32041 ccagttgttc gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca
    32101 tcatacaagt tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt
    32161 ggctgtgagc aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat
    32221 tgaggcgacg taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg
    32281 attaacccga taccccagtt tggcatcggc gacccagtaa ccttctgtaa atgctggcgt
    32341 tcccaccgcg ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac
    32401 agagatatca tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata
    32461 cggcagcgat gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg
    32521 ctgggtatag ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc
    32581 atagccttcg acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac
    32641 ttcattttcg atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac
    32701 ctgccatttg gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt
    32761 ggcactgtta ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg
    32821 ggaaacggca tagttaatat agacattgcc gttttccgtc aggtggtaca gcgccccggc
    32881 tttccagttc accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt
    32941 tgccacacca gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact
    33001 gtcatattca gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt
    33061 gatttgcagc gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc
    33121 gccgttgcgc gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc
    33181 cggtaacgtc accggattaa cgccatagtt agtctgcgtt tcacgggtaa attccacgcc
    33241 ggtactgaca tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt
    33301 ggtgagaatt ttattactca catctttggt attcgccgtg cgtgactagg tccagctatt
    33361 cacatcgctg gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta
    33421 atcctgcttt acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc
    33481 gtgctcaaaa cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc
    33541 gtaaaagtta tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg
    33601 ggcagaatag cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt
    33661 gacatgcaga taattaagat acaaacgatt tgctgtacca aggccaaaag cgacagaagg
    33721 ggcgacgccg taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc
    33781 catcacattc aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc
    33841 gcggcggaac caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg
    33901 ctgcttgctg atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga
    33961 cggcccttta atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat
    34021 atcgcgaata ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc
    34081 gtcgccagtg gtggagttac cgttctcacc cgcaaaaaac gcacccacgc
//
LOCUS       DAZPFP010000029        31187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPFP010000029 DAZPFP010000000
VERSION     DAZPFP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.029"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHUR4_001305"
     CDS             389..2047
                     /locus_tag="ACHUR4_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1454956.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHUR4_001306"
     CDS             complement(2044..3000)
                     /locus_tag="ACHUR4_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1454957.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHUR4_001307"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHUR4_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1454958.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHUR4_001308"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHUR4_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1454959.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUR4_001309"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUR4_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1454960.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUR4_001310"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUR4_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1454961.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUR4_001311"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUR4_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1454962.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUR4_001312"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUR4_001312"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1454963.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHUR4_001313"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHUR4_001313"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUR4_001314"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUR4_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1454964.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUR4_001315"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUR4_001315"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1454965.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUR4_001316"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUR4_001316"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1454966.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUR4_001317"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUR4_001317"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1454967.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUR4_001318"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUR4_001318"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1454968.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUR4_001319"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUR4_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1454969.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUR4_001320"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUR4_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1454970.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUR4_001321"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUR4_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1454971.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUR4_001322"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUR4_001322"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1454972.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHUR4_001323"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHUR4_001323"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1454973.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUR4_001324"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUR4_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUR4_001325"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUR4_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1454974.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHUR4_001326"
     CDS             28042..28833
                     /locus_tag="ACHUR4_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR1454975.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUR4_001327"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUR4_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1454976.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHUR4_001328"
     CDS             29810..30958
                     /locus_tag="ACHUR4_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1454977.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31187
                     /locus_tag="ACHUR4_001329"
     CDS             30976..>31187
                     /locus_tag="ACHUR4_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1454978.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7690 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAZPFP010000030        30096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPFP010000030 DAZPFP010000000
VERSION     DAZPFP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.030"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHUR4_001330"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHUR4_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1454979.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHUR4_001331"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHUR4_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1454980.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHUR4_001332"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHUR4_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1454981.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHUR4_001333"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHUR4_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1454982.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHUR4_001334"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHUR4_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1454983.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHUR4_001335"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHUR4_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1454984.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHUR4_001336"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHUR4_001336"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1454985.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHUR4_001337"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHUR4_001337"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1454986.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="ACHUR4_001338"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="ACHUR4_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1454987.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="ACHUR4_001339"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="ACHUR4_001339"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1454988.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="ACHUR4_001340"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="ACHUR4_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1454989.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="ACHUR4_001341"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="ACHUR4_001341"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1454990.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="ACHUR4_001342"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="ACHUR4_001342"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1454991.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="ACHUR4_001343"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="ACHUR4_001343"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1454992.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="ACHUR4_001344"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="ACHUR4_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1454993.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="ACHUR4_001345"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="ACHUR4_001345"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1454994.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="ACHUR4_001346"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="ACHUR4_001346"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1454995.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="ACHUR4_001347"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="ACHUR4_001347"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1454996.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="ACHUR4_001348"
     CDS             21622..22212
                     /locus_tag="ACHUR4_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1454997.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="ACHUR4_001349"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="ACHUR4_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1454998.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="ACHUR4_001350"
     CDS             22684..23379
                     /locus_tag="ACHUR4_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1454999.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="ACHUR4_001351"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="ACHUR4_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1455000.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="ACHUR4_001352"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="ACHUR4_001352"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1455001.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="ACHUR4_001353"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="ACHUR4_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1455002.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="ACHUR4_001354"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="ACHUR4_001354"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1455003.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="ACHUR4_001355"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="ACHUR4_001355"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1455004.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7843 c         8277 g         6921 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAZPFP010000031        29749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPFP010000031 DAZPFP010000000
VERSION     DAZPFP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.031"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUR4_001356"
     CDS             <1..1224
                     /locus_tag="ACHUR4_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1455005.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUR4_001357"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUR4_001357"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1455006.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUR4_001358"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUR4_001358"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR1455007.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHUR4_001359"
     CDS             2714..3427
                     /locus_tag="ACHUR4_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1455008.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHUR4_001360"
     CDS             complement(3399..4076)
                     /locus_tag="ACHUR4_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1455009.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHUR4_001361"
     CDS             complement(4070..4747)
                     /locus_tag="ACHUR4_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1455010.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHUR4_001362"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHUR4_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHUR4_001363"
     CDS             complement(5443..6021)
                     /locus_tag="ACHUR4_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1455011.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHUR4_001364"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHUR4_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHUR4_001365"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHUR4_001365"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1455012.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUR4_001366"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUR4_001366"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1455013.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUR4_001367"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUR4_001367"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1455014.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUR4_001368"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUR4_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1455015.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHUR4_001369"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHUR4_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1455016.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHUR4_001370"
     CDS             12291..13031
                     /locus_tag="ACHUR4_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1455017.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHUR4_001371"
     CDS             13198..14148
                     /locus_tag="ACHUR4_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1455018.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUR4_001372"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUR4_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUR4_001373"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUR4_001373"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1455019.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUR4_001374"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUR4_001374"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1455020.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUR4_001375"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUR4_001375"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1455021.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUR4_001376"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUR4_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1455022.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUR4_001377"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUR4_001377"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1455023.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHUR4_001378"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHUR4_001378"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1455024.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUR4_001379"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUR4_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1455025.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHUR4_001380"
     CDS             23768..23827
                     /locus_tag="ACHUR4_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1455026.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHUR4_001381"
     CDS             complement(23928..24530)
                     /locus_tag="ACHUR4_001381"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1455027.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHUR4_001382"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUR4_001382"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUR4_001383"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUR4_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1455028.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUR4_001384"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUR4_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1455029.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUR4_001385"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUR4_001385"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1455030.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUR4_001386"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUR4_001386"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1455031.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUR4_001387"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUR4_001387"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1455032.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..>29749)
                     /locus_tag="ACHUR4_001388"
     CDS             complement(29676..>29749)
                     /locus_tag="ACHUR4_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455033.1"
                     /translation="TKSKNHGIRKYNAKTEVKLVYFK"
BASE COUNT         7102 a         7438 c         8028 g         7181 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgt
//
LOCUS       DAZPFP010000032        28914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPFP010000032 DAZPFP010000000
VERSION     DAZPFP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.032"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUR4_001389"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUR4_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1455034.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHUR4_001390"
     CDS             complement(2138..2584)
                     /locus_tag="ACHUR4_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1455035.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHUR4_001391"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHUR4_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1455036.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUR4_001392"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUR4_001392"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1455037.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHUR4_001393"
     CDS             complement(5347..6009)
                     /locus_tag="ACHUR4_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1455038.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHUR4_001394"
     CDS             6182..6595
                     /locus_tag="ACHUR4_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1455039.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUR4_001395"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUR4_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1455040.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUR4_001396"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUR4_001396"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR1455041.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUR4_001397"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUR4_001397"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1455042.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUR4_001398"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUR4_001398"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1455043.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUR4_001399"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUR4_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1455044.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHUR4_001400"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHUR4_001400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUR4_001401"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUR4_001401"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1455045.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUR4_001402"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUR4_001402"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1455046.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUR4_001403"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUR4_001403"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR1455047.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUR4_001404"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUR4_001404"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1455048.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUR4_001405"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUR4_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1455049.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHUR4_001406"
     CDS             14984..15841
                     /locus_tag="ACHUR4_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1455050.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHUR4_001407"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHUR4_001407"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1455051.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHUR4_001408"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHUR4_001408"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1455052.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUR4_001409"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUR4_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1455053.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHUR4_001410"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHUR4_001410"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1455054.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUR4_001411"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUR4_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUR4_001412"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUR4_001412"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1455055.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHUR4_001413"
     CDS             complement(22884..24023)
                     /locus_tag="ACHUR4_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1455056.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHUR4_001414"
     CDS             complement(24069..26165)
                     /locus_tag="ACHUR4_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1455057.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHUR4_001415"
     CDS             complement(26165..26911)
                     /locus_tag="ACHUR4_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1455058.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUR4_001416"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUR4_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1455059.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHUR4_001417"
     CDS             complement(27659..27964)
                     /locus_tag="ACHUR4_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455060.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUR4_001418"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUR4_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1455061.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZPFP010000033        28183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPFP010000033 DAZPFP010000000
VERSION     DAZPFP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.033"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHUR4_001419"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHUR4_001419"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1455062.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUR4_001420"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUR4_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1455063.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUR4_001421"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUR4_001421"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1455064.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUR4_001422"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUR4_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1455065.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUR4_001423"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUR4_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1455066.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUR4_001424"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUR4_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1455067.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUR4_001425"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUR4_001425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1455068.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUR4_001426"
                     /pseudo
     CDS             8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUR4_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUR4_001427"
     CDS             9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUR4_001427"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1455069.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUR4_001428"
     CDS             10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUR4_001428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1455070.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUR4_001429"
     CDS             10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUR4_001429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1455071.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUR4_001430"
     CDS             10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUR4_001430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1455072.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUR4_001431"
     CDS             11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUR4_001431"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1455073.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUR4_001432"
     CDS             12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUR4_001432"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1455074.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUR4_001433"
     CDS             13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUR4_001433"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1455075.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15256..15675
                     /locus_tag="ACHUR4_001434"
     CDS             15256..15675
                     /locus_tag="ACHUR4_001434"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1455076.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUR4_001435"
     CDS             16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUR4_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1455077.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUR4_001436"
     CDS             17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUR4_001436"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1455078.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19549..20370
                     /locus_tag="ACHUR4_001437"
     CDS             19549..20370
                     /locus_tag="ACHUR4_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1455079.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20357..22465
                     /locus_tag="ACHUR4_001438"
     CDS             20357..22465
                     /locus_tag="ACHUR4_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1455080.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22462..24129
                     /locus_tag="ACHUR4_001439"
     CDS             22462..24129
                     /locus_tag="ACHUR4_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1455081.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24132..25658
                     /locus_tag="ACHUR4_001440"
     CDS             24132..25658
                     /locus_tag="ACHUR4_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1455082.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25659..27275
                     /locus_tag="ACHUR4_001441"
     CDS             25659..27275
                     /locus_tag="ACHUR4_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1455083.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27331..27483)
                     /locus_tag="ACHUR4_001442"
                     /pseudo
     CDS             complement(27331..27483)
                     /locus_tag="ACHUR4_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27506..27883)
                     /locus_tag="ACHUR4_001443"
     CDS             complement(27506..27883)
                     /locus_tag="ACHUR4_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455084.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         7297 a         6549 c         7273 g         7064 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcagtctt tctgcctaac
     8941 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
     9001 gcttggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
     9061 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
     9121 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
     9181 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
     9241 acctgcgtac attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca
     9301 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
     9361 gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg
     9421 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     9481 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc
     9541 ctatcgactt gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     9601 ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     9661 tgttctacaa catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     9721 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     9781 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     9841 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     9901 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     9961 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
    10021 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
    10081 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
    10141 ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
    10201 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
    10261 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
    10321 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
    10381 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
    10441 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
    10501 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
    10561 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
    10621 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa
    10681 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
    10741 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
    10801 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
    10861 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
    10921 acgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
    10981 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
    11041 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
    11101 ctggagcagt ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
    11161 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
    11221 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc
    11281 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
    11341 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
    11401 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
    11461 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
    11521 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
    11581 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
    11641 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
    11701 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
    11761 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
    11821 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
    11881 cgtcagacag gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
    11941 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
    12001 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
    12061 tccgctgcac tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt
    12121 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
    12181 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt
    12241 ttctacctcc actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa
    12301 gccttcacca aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc
    12361 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
    12421 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
    12481 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
    12541 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
    12601 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
    12661 ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca
    12721 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct
    12781 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
    12841 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
    12901 caatcctggt aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
    12961 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
    13021 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
    13081 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
    13141 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
    13201 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
    13261 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
    13321 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
    13381 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
    13441 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
    13501 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
    13561 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
    13621 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
    13681 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
    13741 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
    13801 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
    13861 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
    13921 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
    13981 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
    14041 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
    14101 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
    14161 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
    14221 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
    14281 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
    14341 cattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
    14401 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
    14461 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
    14521 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
    14581 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
    14641 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
    14701 cacctccacc aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga
    14761 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
    14821 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
    14881 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
    14941 catcctgcaa cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact
    15001 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
    15061 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
    15121 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
    15181 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
    15241 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
    15301 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
    15361 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
    15421 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
    15481 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
    15541 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
    15601 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
    15661 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct
    15721 ttttttacgc gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac
    15781 ccctgttttc ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta
    15841 aatgaaggta ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca
    15901 ctttcgtcag cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga
    15961 aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga
    16021 cgcgtatgtt gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca
    16081 tgtattccga tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg
    16141 tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg
    16201 gcgatctgct taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc
    16261 agctgggtac gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca
    16321 ttttaatgct ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg
    16381 atgctaaacc ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt
    16441 atggacgtat cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca
    16501 ccgacgagca gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag
    16561 atatgaaacg ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca
    16621 ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac
    16681 gtttaagcga agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt
    16741 accagtccga acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc
    16801 gttttgatct gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta
    16861 tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta
    16921 aaaacagcgt gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga
    16981 atctggcagc ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc
    17041 tggaaggtgc acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca
    17101 cgaaagccgg gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg
    17161 gcgcgggaac cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg
    17221 acgatgtgtt tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg
    17281 cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca
    17341 gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct
    17401 ggccgggtaa tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt
    17461 aactataaaa ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca
    17521 ggttgaccga caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat
    17581 cgcgcaacgt gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg
    17641 atatgactct gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg
    17701 cctcggtaaa gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac
    17761 cggtattgct cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc
    17821 gcatgtttct gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct
    17881 gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat
    17941 tgcccatctg gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg
    18001 tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga
    18061 taccctgcta gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa
    18121 ctttatcgct tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga
    18181 agagggcgat attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc
    18241 ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat
    18301 ttaccgtcac tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct
    18361 taccgggcgc atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga
    18421 actgctgtcg aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg
    18481 tatggtttcc cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc
    18541 ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc
    18601 acagtctggc gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta
    18661 ccttggttcg ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct
    18721 ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac
    18781 tcagttaact gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc
    18841 ctccattgaa catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat
    18901 gctgtctcag gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc
    18961 gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa
    19021 agagatctct tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct
    19081 ggcgctgatt gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga
    19141 aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc
    19201 cgatcaggat gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt
    19261 ggaagaggtg attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt
    19321 cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt
    19381 tgagtaatca ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac
    19441 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
    19501 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
    19561 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
    19621 catggtaaag actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc
    19681 caccgtattt attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt
    19741 gctgtttttc tcagtcttga gtgggagagc agcgtgctag atatacgcga gcagttcccc
    19801 ttattaccta gtgataccag gcagattgca atagatagtg gtattaagca tcctgttatt
    19861 cgtggtgtag atcaggttat gtctactgat tttttagtgg actgcaaaga tggtcctttt
    19921 gagcagtttg ctattcaagt caaacctgca gcagccttac aagacgagcg taccttagaa
    19981 aaactagaac tagagcgtcg ctattggcag caaaagcaaa ttccttggtt catttttact
    20041 gataaagaaa taaatcccgt agtaaaagaa aatattgaat ggctttattc agtgaaaaca
    20101 gaagaagttt ctgcggagct tttagcacaa ctatccccat tggcccatat cctgcaagaa
    20161 aaaggagatg aaaacattat caatgtctgt aagcaggttg atattgctta tgatttggag
    20221 ttaggcaaaa cattgagtga gatacgagcc ttaaccgcaa atggttttat taagttcaat
    20281 atttataagt ctttcagggc aaataagtgt gcagatctct gtattagcca agtagtgaat
    20341 atggaggagt tgcgctatgt ggcaaattaa tgaggttgtg ctatttgata atgatccgta
    20401 tcgcattttg gctatagagg atggccaagt tgtctggatg caaataagcg ctgataaagg
    20461 agttccacaa gctagggctg agttgttgct aatgcagtat ttagatgaag gccgcttagt
    20521 tagaactgat gacccttatg tacatcttga tttagaagag ccgtctgtag attctgtcag
    20581 cttccagaag cgcgaggagg attatcgaaa aattcttcct attattaata gtaaggatcg
    20641 tttcgaccct aaagtcagaa gcgaactcgt tgagcatgtg gtccaagaac ataaggttac
    20701 taaggctaca gtttataagt tgttacgccg ttactggcag cgtggtcaaa cgcctaatgc
    20761 attaattcct gactacaaaa acagcggtgc accaggggaa agacgttcag cgacaggaac
    20821 agcaaagatt ggccgagcca gagaatatgg taagggtgaa ggaaccaagg taacgcccga
    20881 gattgaacgc ctttttaggt tgaccataga aaagcacctg ttaaatcaaa aaggtacaaa
    20941 gaccaccgtt gcctatagac gatttgtgga cttgtttgct cagtattttc ctcgcattcc
    21001 ccaagaggat tacccaacac tacgtcagtt tcgttatttt tatgatcgag aataccctaa
    21061 agctcagcgc ttaaagtcta gagttaaagc aggggtatat aaaaaagacg tacgaccctt
    21121 aagtagtaca gccacttctc aggcgttagg ccctgggagt cgttatgaga ttgatgccac
    21181 gattgctgat atttatttag tggatcatca tgatcgccaa aaaatcatag gaagaccaac
    21241 gctttacatt gtgattgatg tgtttagtcg gatgatcacg ggcttttata tcggctttga
    21301 aaatccgtct tatgtggtgg cgatgcaggc ttttgtaaat gcttgctctg acaaaacggc
    21361 catttgtgcc cagcatgata ttgagattag tagctcagac tggccgtgtg taggtttgcc
    21421 agatgtgttg ctagcggacc gtggcgaatt aatgagtcat caggtcgaag ccttagtttc
    21481 tagttttaat gtgcgagtgg aaagtgctcc acctagacgt ggcgatgcta aaggcatagt
    21541 ggaaagcact tttagaacac tacaagccga gtttaagtcc tttgcacctg gcattgtaga
    21601 gggcagtcgg atcaaaagcc atggtgaaac agactatagg ttagatgcat ctctgtcggt
    21661 atttgagttc acacaaatta ttttgcgtac gatcttattc agaaataacc atctggtgat
    21721 ggataaatac gatcgagatg ctgattttcc tacagattta ccgtctattc ctgtccagct
    21781 atggcaatgg ggtatgcagc atcgtacagg tagtttaagg gctgtggagc aagagcagtt
    21841 gcgagtagcg ttactgcctc gccgaaaggt ctctatttct tcatttggcg ttaatttgtg
    21901 gggtttgtat tactcggggt cagagattct gcgtgagggt tggttgcagc ggagcactga
    21961 tatagctaga cctcaacatt tagaagcggc ttatgaccca gtgctggttg atacgattta
    22021 tttgtttccg caagttggca gccgtgtatt ttggcgctgt aatctgacgg aacgtagtcg
    22081 gcagtttaaa ggtctctcat tttgggaggt ttgggatata caagcacaag aaaaacacaa
    22141 taaagccaat gcgaagcagg atgagttaac taaacgcagg gagcttgagg cgtttattca
    22201 gcaaaccatt cagaaagcga ataagttaac gcccagtact actgagccca aatcaacacg
    22261 cattaagcag attaaaacta ataaaaaaga agccgtgacc tcggagcgta aaaaacgtgc
    22321 ggagcatttg aagccaagct cttcaggtga tgaggctaaa gttattcctt tcaacgcagt
    22381 ggaagcggat gatcaagaag attacagcct acccacatac gtgcctgaat tatttcagga
    22441 tccaccagaa aaggatgagt catgagtgct acccggattc aagcagttta tcgtgatacg
    22501 ggggtagagg cttatcgtga taatcctttt atcgaggcct taccaccatt acaagagtca
    22561 gtgaatagtg ctgcatcact gaaatcctct ttacagctta cttcctctga cttgcaaaag
    22621 tcccgtgtta tcagagctca taccatttgt cgtattccag atgactattt tcagccatta
    22681 ggtacgcatt tgctactaag tgagcgtatt tcggtcatga ttcgaggtgg ctacgtaggc
    22741 agaaatccta aaacaggaga tttacaaaag catttacaaa atggttatga gcgtgttcaa
    22801 acgggagagt tggagacatt tcgctttgag gaggcacgat ctacggcaca aagcttattg
    22861 ttaattggtt gttctggtag tgggaagacg acctctcttc atcgtattct agccacgtat
    22921 cctcaggtga tttaccatcg tgaactcaat gtagagcagg tggtgtattt gaaaatagac
    22981 tgctcgcata atggttcgct aaaagaaatc tgcttgaatt ttttcagagc gttggatcga
    23041 gccttgggct cgaactatga gcgtcgttat ggcttaaaac gtcatggtat agaaaccatg
    23101 ttggctttga tgtcgcaaat agccaatgca catgctttag ggttgttggt tattgatgaa
    23161 attcagcatt taagccgctc tcgttcgggt ggatctcaag agatgctgaa cttttttgtg
    23221 acgatggtga atattattgg cgtaccagtg atgttgattg gtacccctaa agcacgagag
    23281 atttttgagg ctgatttgcg gtctgcacgt agaggggcag ggtttggagc tatattctgg
    23341 gatcctatac aacaaacgca acgtggaaag cccaatcaag agtggatcgc ttttacggat
    23401 aatctttggc aattacagct tttacaacgc aaagatgcgc tgttatcgga tgaggtccgt
    23461 gatgtgtggt atgagctaag ccaaggagtg atggacattg tagtaaaact ttttgtactc
    23521 gctcagctcc gtgcgctagc tttaggcaat gagcgtatta ccgctggttt attgcggcaa
    23581 gtgtatcaag atgagttaaa gcctgtgcac cccatgctag aggcattacg ctcgggtatc
    23641 ccagaacgca ttgctcgtta ttctgatcta gtcgttcccg agattgataa acggttaatc
    23701 caacttcagc tagatatcgc agcgatacaa gaacaaacac cagaagaaaa agcccttcaa
    23761 gagttagata ccgaagatca gcgtcattta tatctgatgc tgaaagagga ttacgattca
    23821 agcctgttaa ttcccactat taaaaaagcg tttagccaga atccaacgat gacaagacaa
    23881 aagttactgc ctcttgtttt gcagtggttg atggaaggcg aaacggtagt gtcagaacta
    23941 gaaaagccct ccaagagtaa aaaggtttcg gctataaagg tagtcaagcc cagcgactgg
    24001 gatagcttgc ctgatacgga tttacgttat atctattcac aacgccaacc tgaaaaaacc
    24061 atgcatgaac ggttaaaagg gaaaggggta atagtggata tggcgagctt atttaaacaa
    24121 gcaggttagc catgagaaac tttcctgttc cgtactcgaa tgagctgatt tatagcacta
    24181 ttgcacgggc aggcgtttat caagggattg ttagtcctaa gcagctgttg gatgaggtgt
    24241 atggcaaccg caaggtggtc gctaccttag gtctgccctc gcatttaggt gtgatagcaa
    24301 gacatctaca tcaaacagga cgttacgctg ttcagcagct tatttatgag cataccttat
    24361 tccctttata tgctccgttt gtaggcaagg agcgccgaga cgaagctatt cggttaatgg
    24421 agtaccaagc gcaaggtgcg gtgcatttaa tgctaggagt cgctgcttct agagttaaga
    24481 gcgataaccg ctttagatac tgccctgatt gcgttgctct tcagctaaat aggtatgggg
    24541 aagccttttg gcaacgagat tggtatttgc ccgctttgcc atattgtcca aaacacggtg
    24601 ctttagtctt ctttgataga gctgtagatg atcaccgaca tcaattttgg gctttgggtc
    24661 atactgagct gctttcagac taccccaaag actccctatc tcaattaaca gcactagctg
    24721 cttatatagc ccctctgtta gatgctccac gagcgcaaga gctttcccca agccttgagc
    24781 agtggacgct gttttatcag cgcttagcgc aggatctagg gctaaccaaa agcaagcaca
    24841 ttcgtcatga cttggtggcg gagagagtga ggcagacttt tagtgatgag gcactagaga
    24901 aactggattt aaagttggca gagaacaagg acacgtgttg gctgaaaagt atattccgta
    24961 agcatagaaa agcctttagt tatttacagc atagtattgt gtggcaagcc ttattgccaa
    25021 aactaacggt tatagaagcg ctacagcagg caagtgctct tactgagcac tctataacga
    25081 caagacctgt tagccagtct gtgcaaccta actctgaaga tttatctgtt aagcataaag
    25141 actggcagca actagtgcat aaataccaag gaattaaggc ggcaagacag tctttagagg
    25201 gtggggtgct atacgcttgg ctttaccgac atgacaggga ttggctagtt cactggaatc
    25261 aacagcatca acaagagcgt ctggcacccg cccctagagt tgattggaac caaagagatc
    25321 gaattgctgt acgacaacta ttaagaatca taaagcgtct agatagtagc cttgatcacc
    25381 caagagcgac atcgagctgg ctgttaaagc aaactcctaa cggaacctct cttgcaaaaa
    25441 atctacagaa actgcctttg gtagcgcttt gcttaaagcg ttactcagag agtgtggaag
    25501 attatcaaat tagacggatt agccaagctt ttattaagct taaacaggaa gatgttgagc
    25561 ttaggcgctg gcgattatta agaagtgcaa cgttatctaa agagcggata actgaggaag
    25621 cacaaagatt cttggaaatg gtttatgggg aagagtgagt ggttaggcta gctacattta
    25681 atgacaatgt gcaggttgta catattggtc atttattccg taactcgggt cataaggagt
    25741 ggcgtatttt tgtttggttt aatccaatgc aagaacggaa atggactcga tttactcatt
    25801 tgcctttatt aagtcgagct aaggtggtta acagtacaac aaagcaaata aataaggcgg
    25861 atcgtgtgat tgagtttgaa gcatcggatc ttcaacgagc caaaataatc gattttccta
    25921 atctctcgtc ctttgcttcc gtacgcaaca aggatggagc gcagagttca tttatttacg
    25981 aagctgaaac accatatagc aagactcgtt atcacatccc acagttagag ctagctcggt
    26041 cattattttt aattaactcc tatttctgtc gaagctgttt gagcagtacc gctttacagc
    26101 aagagttcga cgttcagtat gaggttgagc gagatcattt agagataagg atcttaccca
    26161 gttcatcgtt tcctaaaggg gcgttagagc tgtcggccgt agtgcagctt ttggtttggt
    26221 tgttttcgga tcaagatgtt atggattcgt atgaaagtat ttttaggcac tatcaacaaa
    26281 atagagaaat taagaacggc gttgaaagct ggtgctttag ctttgaccct ccgcccatgc
    26341 agggttggaa attacatgta aaaggacgtt cttctaacga ggataaggat tatttagttg
    26401 aggaaatagt aggtttagaa atcaacgcta tgcttcctag cacaacagct attagccatg
    26461 cctcttttca ggaaaaggag gcaggtgatg gtagtacgca gcacatagcg gtttcaacag
    26521 agtcagttgt tgatgatgag catctacagt tggacgatga ggaaacagcc aatatagaca
    26581 cagacacacg agtcatagag gctgagccga catggataag ttttagtaga cctagtcgaa
    26641 ttgaaaaatc tcgcagggca agaaaaagta gccaaactat tttagaaaaa gaagaagcaa
    26701 caacaagtga aaatagtaat ttggttagta ctgatgagcc acacttaggt ggtgtcctag
    26761 cagcggcaga tgtgggtggg aagcaggatg caaccaatta caactctatt tttgctaatc
    26821 gatttgctgc ttttgatgag ctactttcaa ttctaaaaac taaatttgca tgtcgggtgc
    26881 tttttgaaga aaccttggtt ttgccaaaag ttgggcgtag ccgattacat ctgtgtaaag
    26941 atggctcacc aagagtgatt aaagccgttg gggtgcaacg taatggcagt gaatttgtat
    27001 tgctagaggt ggatgcatcg gatggggtga aaatgctttc taccaaagtg ttgagtggcg
    27061 ttgatagcga aacatggcgg aatgattttg aaaagatacg gcgtggagtg gtgaagagct
    27121 cattgaattg gccaaatagt ttgtttgatc aattatatgg acaagacggg catagagggg
    27181 tgaatcatcc aaaggggttg ggggagctgc aagtatcgag agaggatatg gaagggtggg
    27241 ctgagagagt ggttagagag caatttacgc attaaaggaa tgactgaaag agcctgtaaa
    27301 cccttttgtg taagtgcttt tgccggtcag ttaaaggtgg ccatttaaac ggtcaccaaa
    27361 ttcgatcata aaacggttca tggccggctt ccagttgcgg atcggcatcg tccatttctt
    27421 ggtcgccgcc tggatagcca ggtacaccac cttcatcgct gattcgtccg tagggaacac
    27481 cttgagttca tattaatgga attttctaca aatagcctcc gtggttttga ggggggatta
    27541 cagacgatcc atagtagtaa tccaatgagt tcttgagcgc ggcgacatgt ttggacgcct
    27601 tggcaaaaat tagagcctgc ttgaagtgca ggcgagcacg tgcttggacg attgatccat
    27661 tcgcggtcaa aaactcaatc ttggatgaca gcgtgtcagg aaatccaata tcgaagtccc
    27721 gccccgtgtc aaagtagggc atttcatgat caaaggacga agcttcccag gccacttccc
    27781 taatattcca gccgctagaa acaagatttc gtacgacttc atcaactttt ttgctttcca
    27841 acttgtcaca aagtgcatag gcaagagctt tggcggcaga cacgtgcgtt tcctcttaaa
    27901 cataggtcga acggataccc gaggcaaagc ggagcattag tcccaaagca tctccggtag
    27961 atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga aacttctctc
    28021 tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata tcacacgatc
    28081 cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg tcgttagatt
    28141 gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atc
//
LOCUS       DAZPFP010000034        27788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPFP010000034 DAZPFP010000000
VERSION     DAZPFP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.034"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            153..1022
                     /gene="robA"
                     /locus_tag="ACHUR4_001444"
     CDS             153..1022
                     /gene="robA"
                     /locus_tag="ACHUR4_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1455085.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(1019..1666)
                     /gene="gpmB"
                     /locus_tag="ACHUR4_001445"
     CDS             complement(1019..1666)
                     /gene="gpmB"
                     /locus_tag="ACHUR4_001445"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1455086.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1718..2238
                     /gene="yjjX"
                     /locus_tag="ACHUR4_001446"
                     /pseudo
     CDS             1718..2238
                     /gene="yjjX"
                     /locus_tag="ACHUR4_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(2323..2649)
                     /gene="trpR"
                     /locus_tag="ACHUR4_001447"
     CDS             complement(2323..2649)
                     /gene="trpR"
                     /locus_tag="ACHUR4_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1455087.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2739..4676)
                     /gene="sltY"
                     /locus_tag="ACHUR4_001448"
     CDS             complement(2739..4676)
                     /gene="sltY"
                     /locus_tag="ACHUR4_001448"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1455088.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4887..6554
                     /gene="ettA"
                     /locus_tag="ACHUR4_001449"
     CDS             4887..6554
                     /gene="ettA"
                     /locus_tag="ACHUR4_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1455089.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6661..6828)
                     /gene="nadS"
                     /locus_tag="ACHUR4_001450"
     CDS             complement(6661..6828)
                     /gene="nadS"
                     /locus_tag="ACHUR4_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR1455090.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(6862..8094)
                     /gene="nadR"
                     /locus_tag="ACHUR4_001451"
     CDS             complement(6862..8094)
                     /gene="nadR"
                     /locus_tag="ACHUR4_001451"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1455091.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(8115..9497)
                     /gene="radA"
                     /locus_tag="ACHUR4_001452"
     CDS             complement(8115..9497)
                     /gene="radA"
                     /locus_tag="ACHUR4_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1455092.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9546..10514)
                     /gene="serB"
                     /locus_tag="ACHUR4_001453"
     CDS             complement(9546..10514)
                     /gene="serB"
                     /locus_tag="ACHUR4_001453"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1455093.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10620..11264
                     /locus_tag="ACHUR4_001454"
     CDS             10620..11264
                     /locus_tag="ACHUR4_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1455094.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            11292..12307
                     /gene="lplA"
                     /locus_tag="ACHUR4_001455"
                     /pseudo
     CDS             11292..12307
                     /gene="lplA"
                     /locus_tag="ACHUR4_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            12339..12602
                     /locus_tag="ACHUR4_001456"
     CDS             12339..12602
                     /locus_tag="ACHUR4_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1455095.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(12763..13482)
                     /gene="deoD"
                     /locus_tag="ACHUR4_001457"
     CDS             complement(12763..13482)
                     /gene="deoD"
                     /locus_tag="ACHUR4_001457"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1455096.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(13539..14762)
                     /gene="deoB"
                     /locus_tag="ACHUR4_001458"
     CDS             complement(13539..14762)
                     /gene="deoB"
                     /locus_tag="ACHUR4_001458"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1455097.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(14814..16136)
                     /gene="deoA"
                     /locus_tag="ACHUR4_001459"
     CDS             complement(14814..16136)
                     /gene="deoA"
                     /locus_tag="ACHUR4_001459"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1455098.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(16263..17042)
                     /gene="deoC"
                     /locus_tag="ACHUR4_001460"
     CDS             complement(16263..17042)
                     /gene="deoC"
                     /locus_tag="ACHUR4_001460"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1455099.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            17300..18841
                     /locus_tag="ACHUR4_001461"
     CDS             17300..18841
                     /locus_tag="ACHUR4_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1455100.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            18813..19676
                     /locus_tag="ACHUR4_001462"
     CDS             18813..19676
                     /locus_tag="ACHUR4_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1455101.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(19890..20672)
                     /locus_tag="ACHUR4_001463"
     CDS             complement(19890..20672)
                     /locus_tag="ACHUR4_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1455102.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(20669..21742)
                     /locus_tag="ACHUR4_001464"
     CDS             complement(20669..21742)
                     /locus_tag="ACHUR4_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1455103.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(21864..22025)
                     /locus_tag="ACHUR4_001465"
     CDS             complement(21864..22025)
                     /locus_tag="ACHUR4_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1455104.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(22142..22747)
                     /gene="osmY"
                     /locus_tag="ACHUR4_001466"
     CDS             complement(22142..22747)
                     /gene="osmY"
                     /locus_tag="ACHUR4_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1455105.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(23140..24729)
                     /gene="prfC"
                     /locus_tag="ACHUR4_001467"
     CDS             complement(23140..24729)
                     /gene="prfC"
                     /locus_tag="ACHUR4_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1455106.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(24820..25497)
                     /gene="yjjG"
                     /locus_tag="ACHUR4_001468"
     CDS             complement(24820..25497)
                     /gene="yjjG"
                     /locus_tag="ACHUR4_001468"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1455107.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(25512..25958)
                     /gene="rimI"
                     /locus_tag="ACHUR4_001469"
     CDS             complement(25512..25958)
                     /gene="rimI"
                     /locus_tag="ACHUR4_001469"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1455108.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(25927..26340)
                     /gene="holD"
                     /locus_tag="ACHUR4_001470"
     CDS             complement(25927..26340)
                     /gene="holD"
                     /locus_tag="ACHUR4_001470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1455109.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            26443..27474
                     /gene="rsmC"
                     /locus_tag="ACHUR4_001471"
     CDS             26443..27474
                     /gene="rsmC"
                     /locus_tag="ACHUR4_001471"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1455110.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         6443 a         7657 c         7205 g         6483 t
ORIGIN      
        1 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
       61 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
      121 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
      181 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
      241 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
      301 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
      361 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
      421 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
      481 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
      541 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
      601 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
      661 acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg agtcaggata
      721 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
      781 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
      841 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
      901 tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
      961 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
     1021 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
     1081 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
     1141 tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac
     1201 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
     1261 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
     1321 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
     1381 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
     1441 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
     1501 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
     1561 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
     1621 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
     1681 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
     1741 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
     1801 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac cctttggcag
     1861 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
     1921 ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt tcagttgggt
     1981 ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc cgttacctgc
     2041 ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc gcgttatacc
     2101 ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc
     2161 actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc
     2221 gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat tcggtgcacg
     2281 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa
     2341 cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt tagatccacg
     2401 cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc
     2461 acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt
     2521 cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc
     2581 gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg
     2641 ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc ccggttgtac
     2701 tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc
     2761 gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc atcataagcc
     2821 agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca
     2881 gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta
     2941 taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa
     3001 ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct gctataacca
     3061 ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg
     3121 ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc
     3181 atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa
     3241 gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg
     3301 ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt
     3361 gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag attccagtac
     3421 atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc aacattctgc
     3481 ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc aaccatcggg
     3541 tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg
     3601 cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc
     3661 ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag cgccattcgc
     3721 acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc
     3781 gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc gcgcagctcc
     3841 tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca
     3901 ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg acgggtaaaa
     3961 tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa
     4021 atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc
     4081 aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct
     4141 tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg
     4201 ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat
     4261 ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct
     4321 aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg
     4381 cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga
     4441 ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc
     4501 agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc ctgcttgatt
     4561 tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc
     4621 agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc
     4681 gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa
     4741 ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat
     4801 cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa ctcatcatca
     4861 cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg
     4921 gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg
     4981 gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg ctgcgcatta
     5041 tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg
     5101 gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag
     5161 aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg
     5221 ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca
     5281 ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctgc
     5341 gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtgg
     5401 cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc
     5461 acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc gaaggcaccg
     5521 tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac
     5581 tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg gagcagaaag
     5641 atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag
     5701 agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg
     5761 cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc aacgaactgt
     5821 ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat
     5881 cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcgatcg
     5941 tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctccggtc
     6001 aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg
     6061 atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc
     6121 tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta
     6181 actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt gagcgcggtc
     6241 gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa
     6301 ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg
     6361 gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc
     6421 tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag
     6481 agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc
     6541 gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc cggcctacaa
     6601 aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt aagcgtcgca
     6661 tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt ctacaacagc
     6721 cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat
     6781 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatacctcgc
     6841 ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca ccagctccac
     6901 gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt
     6961 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
     7021 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
     7081 acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt actttttgca
     7141 gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg cgtatttcac
     7201 tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg aatactgcaa
     7261 tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt cccacgcact
     7321 ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
     7381 gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa tatattccca
     7441 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
     7501 atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
     7561 cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt
     7621 gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga aagcatgaat
     7681 acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat ccggcaccgt
     7741 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
     7801 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
     7861 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gccggggaaa
     7921 ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc ttttaatttt
     7981 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
     8041 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
     8101 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
     8161 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
     8221 cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg caccgggcgg
     8281 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
     8341 cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc ggttaccttc
     8401 acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc accgtgacgg
     8461 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
     8521 ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
     8581 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
     8641 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
     8701 tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca
     8761 tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg gccagccagc
     8821 gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt tttggcgaaa
     8881 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
     8941 tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg ctcttcttcg
     9001 gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
     9061 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac
     9121 agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc
     9181 gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc tcctagtacg
     9241 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
     9301 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
     9361 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
     9421 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
     9481 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca
     9541 ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
     9601 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
     9661 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
     9721 gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg
     9781 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
     9841 gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg
     9901 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ctgcgttaag
     9961 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    10021 agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    10081 tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc atcaatacat
    10141 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    10201 gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg tgtagcccgt
    10261 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    10321 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    10381 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    10441 ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag
    10501 gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    10561 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    10621 tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg ttctgtcttg
    10681 ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc
    10741 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg
    10801 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    10861 taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    10921 cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt aaaaaagcag
    10981 gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct
    11041 atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    11101 ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc
    11161 gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca
    11221 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    11281 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    11341 acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc
    11401 tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta
    11461 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc ggtggcggcg
    11521 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    11581 ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
    11641 aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc aaagtctcag
    11701 gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg
    11761 ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    11821 gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggctcacca
    11881 tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga
    11941 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc
    12001 ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    12061 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    12121 ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    12181 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    12241 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    12301 aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg
    12361 aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta
    12421 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    12481 taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa
    12541 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    12601 gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat gaaaaataat
    12661 agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc
    12721 cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag
    12781 cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc
    12841 agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc
    12901 gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc
    12961 gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa
    13021 caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg cgttacgcac
    13081 catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt
    13141 ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt gcggcagaac
    13201 tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt
    13261 ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc
    13321 tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc
    13381 aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc
    13441 agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg
    13501 acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat
    13561 tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca
    13621 cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg aatgtgttca
    13681 cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt gaggatcagg
    13741 atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga
    13801 cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag
    13861 ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac
    13921 agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct
    13981 gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg
    14041 gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg gaagttacca
    14101 gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc
    14161 agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca
    14221 tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc
    14281 atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg
    14341 taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt
    14401 tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat ttcccagtga
    14461 ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact
    14521 tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc
    14581 agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt atcagcttcg
    14641 cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt
    14701 tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc
    14761 atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg
    14821 ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag tttaattgcc
    14881 gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag gataaccgcc
    14941 agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg
    15001 taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac tgccatcccc
    15061 agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc
    15121 agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt
    15181 gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat
    15241 ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat ctccacgcac
    15301 agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag gaactgcacc
    15361 gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt catgtcggtg
    15421 agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg
    15481 gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc
    15541 agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg
    15601 gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc cggagccagt
    15661 gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg
    15721 tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta
    15781 tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc gaccatcggc
    15841 cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc aacaatcggg
    15901 ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc
    15961 agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca
    16021 atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca
    16081 tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta
    16141 acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc
    16201 ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt
    16261 acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag caggcttgcc
    16321 agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt
    16381 tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt
    16441 ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat gatgcgagcg
    16501 ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc
    16561 gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt
    16621 tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc
    16681 aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa caccacgtca
    16741 acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg
    16801 tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt gccctgctct
    16861 ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc ggcggtattg
    16921 ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca
    16981 ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc
    17041 atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa
    17101 catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac ttacaagttt
    17161 gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg
    17221 gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc
    17281 ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca
    17341 gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga
    17401 agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga
    17461 aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc
    17521 cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga
    17581 tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc
    17641 gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta gaattctaac
    17701 acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc
    17761 agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt
    17821 acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc
    17881 tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc
    17941 caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta
    18001 caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat
    18061 tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca
    18121 gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt
    18181 tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt
    18241 tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag ggattgccgc
    18301 gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc
    18361 ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc
    18421 acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga
    18481 tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc
    18541 cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc aggcactggt
    18601 acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg
    18661 taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg
    18721 cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg
    18781 tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtcagta
    18841 agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg
    18901 gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact
    18961 gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg
    19021 tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt ccgcaacatg
    19081 ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg cgcaaagcgg
    19141 tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg
    19201 tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc acctgtctgg
    19261 tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg
    19321 gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta accggacgcg
    19381 ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag ctggcggaac
    19441 tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa gaactggcgg
    19501 cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat gcccacggcg
    19561 tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg
    19621 atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg
    19681 ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg
    19741 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt
    19801 cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca ttatgcgccg
    19861 actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac cgcatacgtg
    19921 ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc gcaaagcacg
    19981 gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc
    20041 atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc gatgacatcg
    20101 cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt gtagcccagc
    20161 tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac accagcacgc
    20221 ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg tgaatgcagg
    20281 atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa ccactgctgc
    20341 ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac cgccaccacc
    20401 ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc gctatgtttt
    20461 tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt ttccgctaac
    20521 gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt gcctacgccc
    20581 gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa
    20641 tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta aatgtcgggt
    20701 cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg actggcgcag
    20761 gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta agcggcgctt
    20821 tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa cgcccaagtt
    20881 tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt aatggcttcg
    20941 gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt
    21001 cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg ctactgtcac
    21061 ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt gacggcacag
    21121 tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc ggaatcgcat
    21181 cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta cgataaaagc
    21241 caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt
    21301 aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg ccgctgtcaa
    21361 acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc tccaccagcc
    21421 agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt
    21481 ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag ataaacgccg
    21541 agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta aactgcgcgc
    21601 gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg ccttcgcaca
    21661 ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg
    21721 ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag aaagacaaag
    21781 taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg gctttggata
    21841 aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa actcactagg
    21901 aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt accggccaga
    21961 ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat gccccaacga
    22021 aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg tgaaaatcac
    22081 tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc gggaggacga
    22141 attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac
    22201 tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga accacgccat
    22261 cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc agtttggctt
    22321 tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct tctttagcgt
    22381 cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc gccactttca
    22441 ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg actttttgat
    22501 cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc gccgccttca
    22561 ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata gagctatcga
    22621 ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg
    22681 tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc agtcttgtca
    22741 tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac acgaggttgc
    22801 attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca tcacaaaagc
    22861 gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat cggtagaatg
    22921 acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac gccatcagaa
    22981 atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat atcagcgaaa
    23041 acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag gaagggaaat
    23101 tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc gggtctggtg
    23161 gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat
    23221 gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac gcttgaactc
    23281 ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt tgactgactc
    23341 atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat caaactgcag
    23401 cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca cctgcaccgc
    23461 gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca gcggatcttt
    23521 caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga acttcatcat
    23581 ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca ggccgaggat
    23641 atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg tcagcgcgtc
    23701 ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt
    23761 accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca tgttggcctg
    23821 aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac gggtatcggt
    23881 ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat gatcaacgcc
    23941 gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc
    24001 tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca gctgctgtgc
    24061 cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa caatgcggac
    24121 ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt tataaaggtg
    24181 gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg cgcagccaat
    24241 tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat cacggtcaag
    24301 tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg
    24361 ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt
    24421 cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac
    24481 caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc cacgctgctt
    24541 ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt ttactgtacc
    24601 ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag ttttaccggc
    24661 gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca aataaggaga
    24721 caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa
    24781 aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac acaggagctg
    24841 ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg
    24901 attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga gaatatcgga
    24961 ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg cctgttccag
    25021 cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg aaatcaccag
    25081 caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca aggcgctaaa
    25141 accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca gagaaaccgc
    25201 gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt taagcaagcc
    25261 tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga
    25321 agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct gatagtcctg
    25381 aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca ggccggtgaa
    25441 tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc acttcattat
    25501 tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc gcgaccgtcc
    25561 gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg
    25621 gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac gccgcgtttt
    25681 tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg ctgatagtcg
    25741 ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt aatcgcaaac
    25801 gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc ctggttgctg
    25861 gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct
    25921 ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt gttgccataa
    25981 cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc
    26041 gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc gactgccttg
    26101 cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc tgacggttaa
    26161 tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat
    26221 caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg
    26281 acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat
    26341 agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac
    26401 atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc atttaccccg
    26461 gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat tctgtttgcc
    26521 ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg tgctcatacc
    26581 cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc ccgttttagt
    26641 ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta ctggccgaag
    26701 aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc agtggggaca
    26761 gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca
    26821 gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt
    26881 ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag cgtcgatggc
    26941 ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt cggtagccag
    27001 ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt cggctgtggc
    27061 gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct caccttgtgc
    27121 gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa
    27181 ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga tatgatcatc
    27241 tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca aacgctgatt
    27301 cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc gaacgccttc
    27361 ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat cgcgcaaacc
    27421 gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg ttgagtatct
    27481 gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg acctacaaaa
    27541 cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc tggcatctgc
    27601 ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc cagcaattac
    27661 acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg cgcctccgtg
    27721 gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt agacgcgcta
    27781 gcttcagg
//
LOCUS       DAZPFP010000035        27601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPFP010000035 DAZPFP010000000
VERSION     DAZPFP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.035"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUR4_001472"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUR4_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1455111.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHUR4_001473"
     CDS             complement(1614..1937)
                     /locus_tag="ACHUR4_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1455112.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUR4_001474"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUR4_001474"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1455113.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUR4_001475"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUR4_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1455114.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUR4_001476"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUR4_001476"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1455115.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUR4_001477"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUR4_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1455116.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHUR4_001478"
     CDS             7537..8574
                     /locus_tag="ACHUR4_001478"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1455117.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUR4_001479"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUR4_001479"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1455118.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUR4_001480"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUR4_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1455119.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUR4_001481"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUR4_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1455120.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHUR4_001482"
     CDS             10745..11626
                     /locus_tag="ACHUR4_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1455121.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUR4_001483"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUR4_001483"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1455122.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUR4_001484"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUR4_001484"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1455123.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUR4_001485"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUR4_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUR4_001486"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUR4_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1455124.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUR4_001487"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUR4_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1455125.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUR4_001488"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUR4_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1455126.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUR4_001489"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUR4_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1455127.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUR4_001490"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUR4_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1455128.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUR4_001491"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUR4_001491"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1455129.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUR4_001492"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUR4_001492"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1455130.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUR4_001493"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUR4_001493"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1455131.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHUR4_001494"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHUR4_001494"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1455132.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHUR4_001495"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHUR4_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1455133.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUR4_001496"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUR4_001496"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1455134.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUR4_001497"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUR4_001497"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1455135.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUR4_001498"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUR4_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1455136.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUR4_001499"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUR4_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1455137.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHUR4_001500"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHUR4_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZPFP010000036        27431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPFP010000036 DAZPFP010000000
VERSION     DAZPFP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.036"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHUR4_001501"
     CDS             <1..576
                     /locus_tag="ACHUR4_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1455138.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUR4_001502"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUR4_001502"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR1455139.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUR4_001503"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUR4_001503"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1455140.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUR4_001504"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUR4_001504"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1455141.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUR4_001505"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUR4_001505"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1455142.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUR4_001506"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUR4_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1455143.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHUR4_001507"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHUR4_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1455144.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHUR4_001508"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHUR4_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1455145.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHUR4_001509"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHUR4_001509"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1455146.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHUR4_001510"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHUR4_001510"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1455147.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHUR4_001511"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHUR4_001511"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1455148.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHUR4_001512"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHUR4_001512"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1455149.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUR4_001513"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUR4_001513"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1455150.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUR4_001514"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUR4_001514"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1455151.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHUR4_001515"
     CDS             13936..14316
                     /locus_tag="ACHUR4_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1455152.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUR4_001516"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUR4_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1455153.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUR4_001517"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUR4_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1455154.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUR4_001518"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUR4_001518"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1455155.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHUR4_001519"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHUR4_001519"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHUR4_001520"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHUR4_001520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHUR4_001521"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHUR4_001521"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHUR4_001522"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHUR4_001522"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHUR4_001523"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHUR4_001523"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUR4_001524"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUR4_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1455156.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUR4_001525"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUR4_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1455157.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUR4_001526"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUR4_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1455158.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUR4_001527"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUR4_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1455159.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUR4_001528"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUR4_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1455160.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUR4_001529"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUR4_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1455161.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUR4_001530"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUR4_001530"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1455162.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUR4_001531"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUR4_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1455163.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUR4_001532"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUR4_001532"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1455164.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUR4_001533"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUR4_001533"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1455165.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUR4_001534"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUR4_001534"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1455166.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6911 a         7266 c         6708 g         6546 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta
    27421 tgttaatcat g
//
LOCUS       DAZPFP010000037        26185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPFP010000037 DAZPFP010000000
VERSION     DAZPFP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.037"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /locus_tag="ACHUR4_001535"
     CDS             complement(<1..214)
                     /locus_tag="ACHUR4_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR1455167.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            524..2296
                     /gene="aspS"
                     /locus_tag="ACHUR4_001536"
     CDS             524..2296
                     /gene="aspS"
                     /locus_tag="ACHUR4_001536"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1455168.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2414..2866
                     /gene="nudB"
                     /locus_tag="ACHUR4_001537"
     CDS             2414..2866
                     /gene="nudB"
                     /locus_tag="ACHUR4_001537"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1455169.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2895..3635
                     /locus_tag="ACHUR4_001538"
     CDS             2895..3635
                     /locus_tag="ACHUR4_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1455170.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3670..4191
                     /gene="ruvC"
                     /locus_tag="ACHUR4_001539"
     CDS             3670..4191
                     /gene="ruvC"
                     /locus_tag="ACHUR4_001539"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1455171.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4193..4795)
                     /locus_tag="ACHUR4_001540"
     CDS             complement(4193..4795)
                     /locus_tag="ACHUR4_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1455172.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4865..4930
                     /gene="yobI"
                     /locus_tag="ACHUR4_001541"
     CDS             4865..4930
                     /gene="yobI"
                     /locus_tag="ACHUR4_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR1455173.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5069..5680
                     /gene="ruvA"
                     /locus_tag="ACHUR4_001542"
     CDS             5069..5680
                     /gene="ruvA"
                     /locus_tag="ACHUR4_001542"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1455174.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5689..6699
                     /gene="ruvB"
                     /locus_tag="ACHUR4_001543"
     CDS             5689..6699
                     /gene="ruvB"
                     /locus_tag="ACHUR4_001543"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1455175.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6846..7631)
                     /gene="znuB"
                     /locus_tag="ACHUR4_001544"
     CDS             complement(6846..7631)
                     /gene="znuB"
                     /locus_tag="ACHUR4_001544"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1455176.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7628..8383)
                     /gene="znuC"
                     /locus_tag="ACHUR4_001545"
     CDS             complement(7628..8383)
                     /gene="znuC"
                     /locus_tag="ACHUR4_001545"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1455177.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8462..9394
                     /gene="znuA"
                     /locus_tag="ACHUR4_001546"
     CDS             8462..9394
                     /gene="znuA"
                     /locus_tag="ACHUR4_001546"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1455178.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9410..10732
                     /gene="mepM"
                     /locus_tag="ACHUR4_001547"
     CDS             9410..10732
                     /gene="mepM"
                     /locus_tag="ACHUR4_001547"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1455179.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10852..11823
                     /gene="lpxM"
                     /locus_tag="ACHUR4_001548"
                     /gene_synonym="msbB"
     CDS             10852..11823
                     /gene="lpxM"
                     /locus_tag="ACHUR4_001548"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1455180.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11954..13396)
                     /gene="pyk"
                     /locus_tag="ACHUR4_001549"
     CDS             complement(11954..13396)
                     /gene="pyk"
                     /locus_tag="ACHUR4_001549"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1455181.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13524..14393)
                     /gene="hexR"
                     /locus_tag="ACHUR4_001550"
                     /gene_synonym="yebK"
     CDS             complement(13524..14393)
                     /gene="hexR"
                     /locus_tag="ACHUR4_001550"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1455182.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14730..16205
                     /gene="zwf"
                     /locus_tag="ACHUR4_001551"
     CDS             14730..16205
                     /gene="zwf"
                     /locus_tag="ACHUR4_001551"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1455183.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16440..18251
                     /gene="edd"
                     /locus_tag="ACHUR4_001552"
     CDS             16440..18251
                     /gene="edd"
                     /locus_tag="ACHUR4_001552"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1455184.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18288..18929
                     /gene="kdgA"
                     /locus_tag="ACHUR4_001553"
                     /gene_synonym="eda"
     CDS             18288..18929
                     /gene="kdgA"
                     /locus_tag="ACHUR4_001553"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1455185.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18985..20163)
                     /gene="purT"
                     /locus_tag="ACHUR4_001554"
     CDS             complement(18985..20163)
                     /gene="purT"
                     /locus_tag="ACHUR4_001554"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1455186.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20297..20587
                     /gene="yebG"
                     /locus_tag="ACHUR4_001555"
                     /pseudo
     CDS             20297..20587
                     /gene="yebG"
                     /locus_tag="ACHUR4_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20654..21010
                     /gene="yebF"
                     /locus_tag="ACHUR4_001556"
     CDS             20654..21010
                     /gene="yebF"
                     /locus_tag="ACHUR4_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1455187.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21337..21996
                     /locus_tag="ACHUR4_001557"
     CDS             21337..21996
                     /locus_tag="ACHUR4_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1455188.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22206..24266
                     /gene="ptrB"
                     /locus_tag="ACHUR4_001558"
     CDS             22206..24266
                     /gene="ptrB"
                     /locus_tag="ACHUR4_001558"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1455189.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24263..24925)
                     /gene="exoX"
                     /locus_tag="ACHUR4_001559"
     CDS             complement(24263..24925)
                     /gene="exoX"
                     /locus_tag="ACHUR4_001559"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1455190.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24949..25605)
                     /gene="yobB"
                     /locus_tag="ACHUR4_001560"
                     /pseudo
     CDS             complement(24949..25605)
                     /gene="yobB"
                     /locus_tag="ACHUR4_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25707..25937)
                     /gene="holE"
                     /locus_tag="ACHUR4_001561"
     CDS             complement(25707..25937)
                     /gene="holE"
                     /locus_tag="ACHUR4_001561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1455191.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26076..>26185
                     /locus_tag="ACHUR4_001562"
     CDS             26076..>26185
                     /locus_tag="ACHUR4_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455192.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6486 c         6818 g         6229 t
ORIGIN      
        1 catcaaccgg ctgttttaat gcttcggcgt aatcggcaga ccagccaact cgcaccagaa
       61 acacgggctg accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct
      121 catcggcagt atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca
      181 ccaacgcagt ggttttagca ttaagttcaa gcatgctgac tcctgaaaaa acaaaatcgt
      241 gcctcacacc ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttttatga
      301 gttcgggcaa cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag
      361 tggtaacgag gcgttccagc gcgggccagg ctcttacggt gcggcagaat ttccagtata
      421 atagccgcct tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc
      481 gcagcagggg gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg
      541 tggacagctc cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg
      601 tcgtcgtgat cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca
      661 ggtatttttc gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga
      721 gttctgcatt caggttacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga
      781 tatggcgacc ggcgaaattg aagtgctggc gtcctcgctg actatcatca accgcgcaga
      841 tgttctgccg cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta
      901 cctcgacctg cgtcgcccgg aaatggcgca acgcctgaaa acccgcgcta aaatcaccag
      961 cctggtgcgc cgttttatgg atgaccacgg cttcctcgac atcgaaactc cgatgctgac
     1021 caaagccacg ccggaaggcg cgcgtgacta cctggtgcct tctcgtgtgc acaaaggtaa
     1081 attctacgca ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggttt
     1141 tgaccgttac tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca
     1201 gcctgaattt actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga
     1261 agtgatggaa gcgctggtgc gtcatttgtg gctggaagtg aagggcgtgg atctgggcga
     1321 tttcccggta atgacctttg cggaagcaga acgccgttat ggttctgata aaccggatct
     1381 gcgtaacccg atggaactga ccgacgttgc cgatctgctg aaatctgttg agtttgcagt
     1441 atttgcgggg ccagcgaacg atccgaaagg ccgtgttgcc gctctacgtg ttccgggtgg
     1501 cgcatcgctg acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc
     1561 gaaaggtctg gcttacatca aagttaacga acgcgcgaaa ggtctggaag gtatcaacag
     1621 cccggtagcg aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc
     1681 gcaagatggc gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgggat
     1741 gggcgcactg ctcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc
     1801 accgctgtgg gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc
     1861 aatgcaccat ccgttcacct caccgaaaga tatgactgct gcagaactga aagctgcacc
     1921 ggaaaatgcg gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg
     1981 ttcagtacgt atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa
     2041 cgaagaggaa cagcgcgaga aattcggctt cctgctcgac gccctgaaat acggtactcc
     2101 gccgcacgca ggtctggcat tcggtcttga ccgtctgacc atgctgctga ctggcaccga
     2161 caatatccgt gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga
     2221 agcaccgagc tttgctaacc cgactgcact ggctgagctg agcattcagg ttgtgaagaa
     2281 ggctgagaat aactgatatg actcaaatac acgaaatcat tcgcgttgca tcaaggcggc
     2341 aactgagtga actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca
     2401 acgaagaggc agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat
     2461 ctatgcacaa gatacgaaac gggtgctgat gttgcagcgg cgtgacgatc ccgatttctg
     2521 gcagtcggta accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga
     2581 agtaaaggaa gaggttacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca
     2641 gcgcacggta gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac
     2701 gcgtaatacg gaatcatggt tctgtcttgc gcttccgcac gagcggcaga tcgttttcac
     2761 tgaacatctg gcttacaagt ggcttgatgc gcctgctgcg gcggcgctca ctaagtcctg
     2821 gagcaaccgg caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat
     2881 ctggagatat ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg
     2941 cgcaggatgc taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg
     3001 ctaagctggg cggtggcgat ccggacgcta acccgcgtct gcgtgcggcg gtggataaag
     3061 cactgtctaa caacatgacc cgtgacacac tgaaccgcgc aattgcacgt ggtgtgggcg
     3121 gtgatgatga tgcaaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg
     3181 caatcatgat tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcacg
     3241 cattcagcaa atgtggcggt aacctcggca ctgacggttc cgtagcttat ctgttcagca
     3301 aaaaaggcgt gatctccttc gagaaaggcg acgaagacac catcatggaa gcagctctgg
     3361 aagcaggtgc tgaagacgtt gtgacatatg atgacggcgc gattgatgtc tacactgcat
     3421 gggaagaaat gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg
     3481 cggaagtttc catgatcccg tccaccaaag ctgatatgga tgcagaaacc gcgccgaaac
     3541 tgatgcgtct gatcgatatg ctggaagatt gcgacgacgt gcaggaagtt taccataacg
     3601 gtgaaatctc tgatgaggtt gcagcgactc tctgatgatg cctgctaaac agcaaaacgg
     3661 agacgcgtga tggctattat tctcggcatt gacccgggtt cgcgcgtgac cggctacggc
     3721 gtcatccgcc aggtaggtag gcaactgtcc tacctgggta gcggatgcat acgcaccaaa
     3781 gtggatgatt taccgtctcg tctgaagctc atctatgcgg gcgtgacgga aatcatcacc
     3841 cagttccagc ctgattattt cgccattgaa caagtcttta tggcgaagaa cgctgactca
     3901 gccctgaaac tgggccaggc gcgcggcgtg gcgattgttg cggcggtgaa tcaggaattg
     3961 ccggtatttg aatacgcggc acgtcaggta aagcaaacgg tggtaggtat gggtagtgcc
     4021 gaaaaaagcc aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag
     4081 gcggatgccg ccgatgcgct ggcgattgcc atcacccact gccacgttag tcagaatgcg
     4141 atgcagatga gcgaatcgcg gctgaacctg acgagagggc gactgcgtta agttataccg
     4201 cctcggtcag ttccggctga ggcgtttcaa ctccctccgc gtgaatcaac accaaacccg
     4261 gatgatgcca caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacctaaga
     4321 accagaattt ccagaaagtg agttttgcat tcggattgct atttaacagt tgtccaaacg
     4381 tctctatttc acccaccgga atacatagcg cctctttata aatatcaaag acaaattttg
     4441 aacagaactg gcgcgaagat tcgtatttaa aacccgtgtg gtaaagtttg cgtagccggg
     4501 agggaacctg ttcaacaatt cgttgttttt gttgttctgt cagcccagcg tctaatcgct
     4561 ttatagcata gcgttgatta gacgaacgtt taataaaacg agatagcgtg gtgatggttg
     4621 aaaggggaac ccggctttct gcaaccagga agtcttcacc gttatgaccg ataatgatcc
     4681 cgacgtgatt actccagcaa tttgatgcag ctgatatttg accaaataag gcagcaccta
     4741 tacatgtaaa gacgatatct ccaatttcat attcagcagg gtaattaata ttcacgttgt
     4801 cattccattg aaatagatac aaatatacgt aatagcataa aaaaattatt tttcaggagt
     4861 cgcaatgtat atttttatta cgcatttctt cactgaatat gtaatattca aatatttgct
     4921 tccaatataa cctgttgaat aaattatact gtgccatttt tcagatcatc gggacacctc
     4981 gcaagttttc ttcatccttc gctggatatc tatccagcat ttttttatca tacagcatta
     5041 tctttgattc attacgcagg agcgtcatgt gataggcaga ctcagaggca tcatcattga
     5101 aaaacaaccc ccgctggtgt taattgaagt gggcggcgta ggctatgaag tgcatatgcc
     5161 gatgacctgt ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt tcacccactt
     5221 tgtggtgcgt gaagacgcgc aactgctgta cggttttaac aataaacaag aacgcacgtt
     5281 gttcaaagag ttgatcaaaa ccaacggcgt cggcccgaag ttggccctgg cgatcctctc
     5341 cggaatgtca gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg gggcactggt
     5401 gaaactgccg ggtattggca aaaaaaccgc cgaacgcttg attgttgaaa tgaaagaccg
     5461 atttaaaggt ttgcatggcg atctctttac gccagccgcc gacctggtac tcacgtctcc
     5521 tgctagcccg gcgaccgacg atgctgaaca agaagcagtt gccgcgctgg tggcgctagg
     5581 ctataaacca caagaagcaa gccgcatggt gagcaaaatc gctcgccctg acaccagcag
     5641 tgaaacatta attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat gattgaagca
     5701 gaccgtctga tttctgccgg taccactttg ccggaagatg tagcagatcg cgccattcgc
     5761 cctaaattac tggaagagta tgttggtcag ccgcaggttc gttcacagat ggagattttc
     5821 atcaaagcag cgaaactgcg cggcgatgcc ctcgatcatt tgttgatttt tggtcctccg
     5881 gggttgggta aaactacgct tgccaacatt gtcgccaatg aaatgggcgt taatttacgc
     5941 acgacttctg gtccggtgct ggaaaaggcg ggcgatttgg ctgcgatgct cactaacctt
     6001 gaaccgcatg acgtgctgtt tattgatgag atccaccgtt tatcgccagt tgttgaagaa
     6061 gtgctgtacc cggcaatgga agactaccaa ctggatatca tgattggtga aggtccggcg
     6121 gcacgctcca ttaaaattga tttgccgccg tttaccctga ttggcgcaac cacgcgcgca
     6181 ggttcgctga catcaccgtt gcgcgaccgt tttggtattg tgcaacgtct ggagttttat
     6241 caggtgccgg atctgcaata tatcgtcagt cgcagtgcac gctttatggg gcttgagatg
     6301 agtgatgacg gtgcgcagga agtagctcgt cgcgctcgcg gtacgccacg tattgccaac
     6361 cgtctgctgc gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac catctcggca
     6421 gatatcgctg ctcaggcgct ggatatgttg aatgtcgatg ctgaaggttt cgattatatg
     6481 gaccgcaaat tgttgctggc ggtaatcgat aagttctttg gtgggccggt aggtctggat
     6541 aacctggcgg cagccattgg cgaagaacgt gaaaccattg aagatgtact ggaaccttat
     6601 ttgattcagc aaggcttttt gcagcgtaca ccgcgcgggc gtatggcgac gacgcgggcg
     6661 tggaatcact ttggcataac gccgccagaa atgccgtaag tcggattggt cgctcgcgcc
     6721 gcatccgaca ctggcagtgt gtctgatgcg acactgttcg cgtcttatca ggccagccag
     6781 agatagcgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat gtgttcagcg
     6841 atagattagc tggcctgctt tttcatcata ctgataataa ataacagtgc cgcacatagc
     6901 accaccgacg gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc gccagttact
     6961 gccaccatcc ccaccaaaac agcgacacca gccatctgtt ccggcgtacg ggcaaagcga
     7021 cgcgcagtag ctgcaggaat aatcagcaac gaagtaataa tcaacgcacc gacaaatttc
     7081 atcgctacac caatcgtcaa tgccgtcacc agcatcaaca acaatttcac ttgctgtaat
     7141 ttcacaccat caacaaacgc cagatccggg ctaatcgtca tcgacagcaa attgcgccat
     7201 tgccagaaca aaatagccac cacgatgacc acgccaatcg caatagagat gagatcttct
     7261 ggcgtcactg ccagtaaatc accgaacagg taagccatca aatcaacacg aatgttagac
     7321 atcagactaa cgaccactag gcccagcgac agggcactgt gcgccataat ccctaataac
     7381 gtgtcgatcg ccagctgtgg acgcttctcc agccatacca gaccgcccgc cagcagcagc
     7441 gtaacggcaa tcaccgcata gaatggattc acgtccagca acaaaccaaa cgcgacacca
     7501 agtaatgagg catgagccag cgtatcaccg aaataagaca tacgacgcca gactacaaac
     7561 gaacccagcg gacccgcggc acaggcgagc atgatcccgg ctaaccaacc gggaaataat
     7621 aattcaatca tgagcgatca tttccccgac gcaaaacaat tcgtccctgt aaatcgtgac
     7681 gatgattatg atgatggcga tagataccca gttgttcagc accacgagga ccaaacattg
     7741 aaataaactc cggatgcagg gaaacaactt ccggtgtgcc ggaacaacaa atgtggtgat
     7801 tcaggcacag cacttcatcg gtttttgcca tcaccagatg cagatcgtga gaaaccatta
     7861 aaacgccaca atccagttca cgacgcaact ggtcaataag gtcatataac gccacttggc
     7921 catttacatc cacgccttga gtgggttcat ccagcactaa taattgcggg caatttaaca
     7981 atgctcgcgc taatagtaca cgctgcgttt cgccaccaga gagcttttgc atcggtgcgt
     8041 taatcaggtg cccggcctgg acacgtttca gtgcaggcaa aatatcttct ttatgtgtac
     8101 cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg agatacagct
     8161 tctgcggtac atagccgatg cgcagttttc cgttgcgctt gataacccct tcatcgggtg
     8221 ttaccagccc gagcactacc cgtaccagtg tcgacttacc tgcgccattt ggcccaagta
     8281 aagtcaaaat ttttccaggt ttaagttcca gcgacacatc agagaggacg cggcgttggc
     8341 caaaagaaac cgagacattt tccagggaaa ccagacttgt catgttaatt ttagtcttgc
     8401 agtagtcatg aaatgttata atatcacact tctcatattc attacgatga ttggtcgcat
     8461 tatgttacat aaaaaaacgc ttcttttcgc agcattatcc gccgctctct ggggaggtgc
     8521 aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt tcatcgcttc
     8581 tgccattgct gatggtgtaa cagaaacgga ggttttactg cctgacggcg cttcagaaca
     8641 tgattattca ctgcgtccat cggatgtaaa acgcttacag aacgcggact tagtcgtttg
     8701 ggttggcccg gagatggaag cgtttatgca aaaaccggta agcaaattac cagaagcgaa
     8761 gcaggtaacg attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa gtattcacgg
     8821 cgatgatgat gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc acggcgattt
     8881 caacatgcat ctttggcttt ccccagagat agcgcgggct acagcggttg caatccatgg
     8941 aaaattagtg gaacttatgc cgcaaagtcg agccaaactt gacgccaacc tgaaggattt
     9001 tgaggcacaa ttggcctcaa ccgaaacgca ggttggtaac gagctcgcgc cgctcaaggg
     9061 gaaaggttat ttcgtttttc acgatgctta cggctatttc gaaaaacagt tcggactgac
     9121 accgcttggt cattttaccg ttaaccctga gattcaacct ggcgcgcagc gtttacatga
     9181 aataagaaca cagttggttg agcaaaaagc aacctgcgtt tttgctgagc cacagttcag
     9241 gccagcggtc gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa cgttggatcc
     9301 cctggggacg aatatcaaac tgggtaaaac aagctattca gaattcctga gtcaattagc
     9361 caaccagtat gcgagctgcc tgaaaggaga ttaatgagga agtgattacg tgcaacagat
     9421 agcccgctct gtcgccctgg cgtttaataa tttaccgcga ccacaccgcg ttatgttggg
     9481 gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg ccctatgttt atcaccgtga
     9541 tgccacgcca attgtcaaaa ccattgagct ggaacagaac gaaattcgtt cgctcttacc
     9601 tgaagccagt gagccgattg atcaagctgc acaagaagat gaagccattc cccaggacga
     9661 actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt ccactggcga
     9721 tacgctaagc agcattctca atcagtatgg tattgatatg ggtgatatca cccaacttgc
     9781 tgctgccgac aaagaattgc gtaacctgaa aatcggtcaa caactctcct ggacattaac
     9841 cgcggacggc gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa cccgaaccta
     9901 tgaccgtact gccgctaacg gttttaaaat gaccagcgaa atgcagcaag gagagtgggt
     9961 taacaatctg ctgaaaggta ccgtcggagg gagctttgtt gccagcgcca gaaacgccgg
    10021 tttaaccagc gccgaagtga gcgcagtgat taaagccatg cagtggcaaa tggatttccg
    10081 caaactgaaa aaaggcgatg aatttgcggt attaatgtct cgagaaatgc ttgatggtaa
    10141 acgtgagcaa agccagctgc tgggcgtacg tttgcgttca gaaggtaaag attattacgc
    10201 aatccgcgct gaggatggca aattctacga tcgtaacggt actggtctgg cgaaaggatt
    10261 cttgcgattc ccgacggcga aacagttccg tatctcgtct aactttaacc cgcgtcgaac
    10321 taatccggtg acaggtcgcg ttgcaccaca cagaggtgtt gatttcgcca tgccacaagg
    10381 tacgccagtg ctttcagtgg gtgacggtga agtggtggtt gccaaacgca gtggcgcagc
    10441 aggttattat gtggctattc gtcatggtcg cagctacacc acgcgttata tgcacttgcg
    10501 caagattctg gtgaaaccgg gacagaaggt gaaacgtggc gaccgtatcg cgctttccgg
    10561 taataccgga cgttcaactg ggccgcatct gcactatgaa gtatggataa accagcaggc
    10621 cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg ctgaccggct ccgatcgtcg
    10681 cgaattcctg gcgcaggcca aagagattgt gccgcagcta cggtttgatt aattaacatc
    10741 cattcgcagc cggtacgcag tcagtaccgg ctttttttat ttggtgcggg gcaagttgtg
    10801 ccgctacact atcaccagat tgatttttgc cttatccgaa actggaaaag catggaaacg
    10861 aaaaaaaata atagcgaata cattcctgag tttgataaat cctttcgcca cccgcgctac
    10921 tggggagcat ggctgggtgt agcagcgatg gcgggtatcg ctttaacgcc gccaaagttc
    10981 cgtgatccca ttctggcacg gctgggacgt tttgccggac gactgggaaa aagctcacgc
    11041 cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac gtagtgaagc tgaacgcgaa
    11101 gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat ggctgagttg
    11161 gcaatacgcg ggccggagaa aattcagccg cgcgttggct ggcaagggct ggagatcatc
    11221 gaagagatgc ggcgtaataa cgagaaagtt atctttctgg tgccgcacgg ttgggccgtc
    11281 gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat gttccataat
    11341 cagggcaacc cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt tggcggtcgt
    11401 ctgcatgcga gaaatgacgg tattaaacca ttcatccagt cggtacgtca ggggtactgg
    11461 ggatattatt tacccgatca ggatcatggc ccagagcaca gcgaatttgt ggatttcttt
    11521 gccacctata aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtatg ccgtgcgcgc
    11581 gttgtaccgc tgtttccgat ttatgatggc aagacgcatc gtctgacgat tcaggtgcgc
    11641 ccaccgatgg atgatctgtt agaggcggat gatcatacga ttgagcggcg gatgaatgaa
    11701 gaagtcgaga tttttgttgg tccgcgacca gaacaataca cctggatact aaaattgctg
    11761 aaaactcgca aaccgggcga aatccagccg tataagcgca aagatcttta tcccatcaaa
    11821 taaaaaagcc tctcgcgagg agaggccttc gcctgatgat aagttcaagt ttgcttcaga
    11881 atattcgaaa tctgttgaac tatcattgaa ctataggccg gatgtggcgt tttcgccgca
    11941 tccggcaacg tacttactct accgttaaaa tacgcgtggt attagtagaa cccacggtac
    12001 tcatcacgtc gccttgggtg acaatcacca ggtcaccaga catcaagtaa cctttatcgc
    12061 gcagcagatt aaccgcttcg ctggcagctg ctacgccgtc attagcgcta tcaaagggca
    12121 ccggcgtaac gccacgatag agagcagtca ggttcagcgt acgttcatgg cgcgacatgg
    12181 cgaaaattgg tagaccagag ctgatacggg aggtcatcag cgcggtacga cccgattcgg
    12241 tcatggtaat gatcgccgta acgcccttca ggtggttagc tgcgtacatt gccgacatgg
    12301 caatagcttc ttccacattg tcgaactgaa cgtccagacg gtgtttagaa acgttgatgc
    12361 tcgggatttt ttccgcaccc aggcaaacgc gcgccatggc tgcaacagtt tctgacggat
    12421 actgcccagc ggcagtttct gcagacagca tcacagcgtc agtaccatcc agaacggcgt
    12481 ttgctacgtc catgacttct gcacgcgtcg gcatcgggtt agtaatcatt gactccatca
    12541 tctgggtcgc cgtgattacc gctcggttta gctgacgcgc acgacggatc aacgctttct
    12601 gaatgccgac cagttccggg tcgccaattt ccacaccgag gtcgccacgt gcaaccatta
    12661 ccacgtcaga ggcgaggatg atgtcatcca ttgcatcctg gctgcaaacg gcttccgcac
    12721 gttcaacctt ggcaacaatt ttcgcatcac atcctgcatc gcgtgccagg cgacgggcat
    12781 agttcagatc ttcgccacag cgtgggaagg agacagccag gtaatctacg ccaatcaacg
    12841 ccgcagtctt aatgtctgct ttgtcttttt cggtcagcgc ttcagccgac aaaccgccgc
    12901 caagtttgtt gataccttta ttgttggaga ggggaccacc gacggtgact tcggtgaaca
    12961 ctttcatgcc ctgaacttcc agtactttta actggacgcg accatcgtcc agcagcagga
    13021 tgtcaccagg cacgacgtca gtaggcaggc ctttgtagtc gataccgact ttttctttgt
    13081 cgccttcacc tttacccagg ttggcgtcga gcaggaattt atccccaata ttgaggaaaa
    13141 ctttgccttc tttaaaggtg gatacacgga ttttgggccc ctggaggtca cccagaatag
    13201 ccacatgacg ccccagtttt gcggcaatct cacgaacttt atccgcgcgc attttgtgat
    13261 cttcaggcga gccgtgagaa aagttcatac gtacaacgtt ggcacccgcc gcgataactt
    13321 tttcaagatt attatcgcga tctgttgctg ggcctaacgt ggtaacgatt tttgttctgc
    13381 gaagcctttt ggacatgtaa tactccgttg actgaaacaa ccaggtgttg cttgaacatg
    13441 aaatccgaat gaaataacgc cgcgatggcg ataaattaat ctcattcgga tgtcgtataa
    13501 gattaggaca gtgacagtcg cttttagcga tcgtcactta aattaagtaa ctgcttatca
    13561 aaacgcgatt ccttcagcgc ttccttgacc cgcttcaagt tatctctgaa ttttgcaccg
    13621 cgtcgcaaag taaatcctgt cgccagcaca tctatcacgg tcagctgtgc aagtcgagaa
    13681 accatgggca tataaatgtc agtatcttcc ggtacgtcga gggtaattgc cagcgttgct
    13741 tcccgggcga gcggggtacc cgcagaggtg agggcaatca ccatggcgtc gttttcgcgt
    13801 gccagctgcg ccagctcgac cagatttttt gttcttcctg tgtgagaaat cagcaccacc
    13861 acgtctccgt cgctacaatt catacaactc atgcgttgca gcacgatatc atcggagtac
    13921 accaccggaa cattaaaacg aaagaactta ttcatcgcat cgtgggcaac ggcggcagaa
    13981 gagcctaatc cgaaaaaggc gatttttttt gcctgagtga gcaagtcgac ggcgcggttg
    14041 atggcagatt tatccagtga atgacggaca tgatcgagcg ttgccattgc ggactcaaat
    14101 attttccccg tgtatgattc aacgctgtca tcttcattga cgttgcgatt aacataggga
    14161 gtgccattcg ccagactttg tgccagatga agtttaaaat caggaaaacc gcgcgtgtcc
    14221 atgctgcgac agaaacgatt caccgtcggt tcgctaacat tggcttccag cgccagagca
    14281 gcaatactcg aatggatcgc gttatcgggc gaagccagaa tgacctcggc aactttgcgc
    14341 tctgatttgc tcaaatgttc cagctgagac tggatttttt ccagcatatt catgatgtaa
    14401 agagactcac gggtaatgac gatttccgca ctgaaagaaa tcgaaatgca gttttgtcag
    14461 atattacgcc tgtgtgccgt gttaatgaca aaagcagata aaaaagttgt tatttttttc
    14521 ataacatgat cagtgtcaga tttttaccga atggaaaacg atgatttttt tatcagtttt
    14581 gccgcgcttt gcgcgctttt cccgtaatcg cacgggtgga taagcgttta cagttttcgc
    14641 aagctcgtaa aagcagtaca gtgcaccgta agaaaattac aagtataccc tggcttaagt
    14701 accgggttag ttaacttaag gagaatgaca tggcggtaac gcaaacagcc caggcctgtg
    14761 acctggtcat tttcggcgcg aaaggcgacc ttgcgcgtcg taaattgctg ccttccctgt
    14821 atcaactgga aaaagtcggt cagctcaacc cggacacccg gattatcggc gtagggcgtg
    14881 ctgactggga taaagcggca tataccaaag ttgtccgcga ggcgctcgaa actttcatga
    14941 aagaaaccat tgatgaaggt ttatgggaca ccctgagtgc acgtctggat ttttgtaatc
    15001 tcgatgtcaa tgacactgct gcattcaacc gtctcggcgc gatgctggat caaaaaaatc
    15061 gtatcactat taactacttt gccatgccgc ccagcacttt tggcgcaatt tgcaaagggc
    15121 ttggcgaggc aaaactgaat gctaaaccgg cacgcgtagt catggagaaa ccgctgggga
    15181 cgtcgctggc gacctcgcag gaaatcaatg atcaggttgg cgaatacttc gaggagtgcc
    15241 aggtttaccg tatcgaccac tatcttggta aagaaacggt gctgaacctg ttggcgctgc
    15301 gttttgctaa ctccctgttt gtgaataact gggacaatcg caccattgat catgttgaga
    15361 ttaccgtggc agaagaagtg gggatcgaag ggcgctgggg ctattttgat aaagccggtc
    15421 agatgcgtga catgattcag aaccacctgc tacaaattct ctgcatgatt gcgatgtctc
    15481 cgccatctga cctgagcgca gacagcatcc gcgatgaaaa agtgaaagta ctgaagtccc
    15541 tgcgccgcat cgaccgctcc aacgtacgcg aaaaaaccgt acgcgggcaa tatactgcgg
    15601 gcttcgccca gggcaaaaaa gtgccgggat atctggaaga agagggcgcg aacaaaagca
    15661 gcaatacaga aaccttcgtg gcgatccgcg tcgacattga taactggcgc tgggccggtg
    15721 tgccattcta cctgcgtact ggtaaacgtc tgccgaccaa atgttctgaa gtcgtggtct
    15781 atttcaaaac acctgaactg aatctgttta aagagtcgtg gcaggatctg ccgcagaata
    15841 aactgactat ccgtctgcaa cctgatgaag gcgtggatat ccaggtactg aataaagttc
    15901 ctggccttga ccacaaacat aacctgcaaa tcaccaagct ggatctgagc tattcagaaa
    15961 cctttaatca gacgcatctg gcggatgcct atgaacgttt gctgctggaa accatgcgtg
    16021 gtattcaggc actgtttgta cgtcgcgacg aagtggaaga agcctggaaa tgggtagact
    16081 ccattactga ggcgtgggcg atggacaatg atgcgccgaa accgtatcag gccggaacct
    16141 ggggacccgt tgcctcggtg gcgatgatta cccgtgatgg tcgttcctgg aatgagtttg
    16201 agtaatatca gcgcttatcc tttatgggta ttttaccggt aacatgatct tgcacagatt
    16261 gtagaacaat ttttacactt tcaggcctcg tgcggattca cccacgaggc tttttttatt
    16321 acactgactg aaacgttttt gccctatgag ctccggttac aggcgtttca ctcataaatc
    16381 ctctgaatga aacgcgttgt gaatcatcct gctctgacaa ctcaatttca ggagccttta
    16441 tgaatccaca attgttacgc gtaacaaatc gaatcattga acgttcgcgc gagactcgct
    16501 ctgcttatct cgcccggata gaacaagcga aaacgtcgac cgttcatcgt tcgcagttgg
    16561 catgcggtaa cctggcacac ggtttcgctg cctgccagcc agaaaacaaa gcctctttga
    16621 aaagcatgtt gcgtaacaat atcgccatca tcacctccta taacgacatg ctctccgcgc
    16681 accagcctta tgaacactat ccagaaatca ttcgtaaagc cctgcatgaa gcgaatgcgg
    16741 ttggtcaggt tgcgggcggt gttccggcga tgtgtgatgg tgtcacccag gggcaggatg
    16801 gaatggaatt gtcgctgcta agccgcgaag tgatagcgat gtctgcggcg gtggggctgt
    16861 cccataacat gtttgatggt gctctgttcc tcggtgtgtg cgacaagatt gtcccgggtc
    16921 tgacgatggc agccctgtcg tttggtcatt tgcctgcggt gtttgtgccg tctggaccga
    16981 tggcaagcgg tttgccaaat aaagaaaaag tgcgtattcg ccagctttat gccgaaggta
    17041 aagtggaccg catggcctta ctggagtcag aagccgcgtc ttaccatgcg ccgggaacat
    17101 gtactttcta cggtactgcc aacaccaacc agatggtggt ggaatttatg gggatgcagt
    17161 tgccaggctc ttcttttgtt catccggatt ctccgctgcg cgatgctttg accgctgccg
    17221 ctgcgcgtca ggttacacgc atgaccggta atggtaatga atggatgccg atcggtaaga
    17281 tgatcgatga gaaagtggtg gtgaacggta tcgttgcact gctggcgacc ggtggttcca
    17341 ctaaccacac catgcacctg gtggcgatgg cgcgcgcggc cggtattcag attaactggg
    17401 atgacttctc tgacctttct gatgttgtac cgctgatggc acgtctctac ccgaacggtc
    17461 cggccgatat taaccacttc caggcggcag gtggcgtacc ggttctggtg cgtgaactgc
    17521 tcaaagcagg cctgctgcat gaagatgtca atacggtggc aggctttggt ctgtctcgtt
    17581 atacccttga accatggctg aataatggtg aactggactg gcgggaaggg gcggaaaaat
    17641 cactcgacag caatgtgatc gcttccttcg aacaaccttt ctctcatcat ggtgggacaa
    17701 aagtgttaag cggtaacctg ggccgtgcgg ttatgaaaac ctctgccgtg ccggttgaga
    17761 accaggtgat tgaagcgcca gcggttgttt ttgaaagcca gcatgacgtt atgccggcct
    17821 ttgaagcggg tttgctggac cgcgattgtg tcgttgttgt ccgtcatcag gggccaaaag
    17881 cgaacggaat gccagaatta cataaactca tgccgccact tggtgtatta ttggaccggt
    17941 gtttcaaaat tgcgttagtt accgatggac gactctccgg cgcttcaggt aaagtgccgt
    18001 cagctatcca cgtaacacca gaagcctacg atggcgggct gctggcaaaa gtgcgcgacg
    18061 gggacatcat tcgtgtgaat ggacagacag gcgaactgac actgctggta gacgaagcgg
    18121 aactggctgc tcgcgaaccg cacattcctg acctgagcgc gtcacgcgtg ggaacaggac
    18181 gtgaattatt cagcgccttg cgtgaaaaac tgtccggtgc cgaacagggc gcaacctgta
    18241 tcacttttta agacgacaaa tttgtaatca ggcgagagaa aactctgatg aaaaactgga
    18301 aaacaagtgc agaatcaatc ctgaccaccg gcccggttgt accggttatc gtggtaaaaa
    18361 aactggaaca cgcggtgccg atggcaaaag cgttggttgc tggtggggtg cgcgttctgg
    18421 aagtgactct gcgtaccgag tgtgcagttg acgctatccg tgctatcgcc aaagaagtgc
    18481 ctgaagcgat tgtgggtgcc ggtacggtgc tgaatccaca gcagctggca gaagtcactg
    18541 aagcgggtgc acagttcgca attagcccgg gtctgaccga gccgctgctg aaagctgcta
    18601 ccgaagggac tattcctctg attccgggga tcagcactgt ttccgaactg atgctgggta
    18661 tggactacgg tttgaaagag ttcaaattct tcccggctga agctaacggc ggcgtgaaag
    18721 ccctgcaggc gatcgcgggt ccgttctccc aggtccgttt ctgcccgacg ggtggtattt
    18781 ctccggctaa ctaccgtgac tacctggcac tgaaaagcgt gctgtgcatc ggtggttcct
    18841 ggctggttcc ggcagatgcg ctggaagcgg gcgattacga ccgcattact aagctggcgc
    18901 gtgaagctgt agaaggcgct aagctgtaaa agtcaaaatg cccgatctag gatcgggcat
    18961 ttttgtagcg tttttttgcc gggtttaacc ctgtactttt acctgtccgg cggcgtgctt
    19021 cgcgcgttca atggcgtcaa caacactctc tgaagtagcc agtaccaccc ccagatgacg
    19081 gctgccatca atttccggct taccaaataa acgaatctgc aaatctgcgc ctacggcatt
    19141 ctgcacatta tcaaacgtga cattctgact ggtcagttgt ggcagaataa cggcagaagc
    19201 tgcaggacca tactgacgga tcccgccaac aggaagtccg aggaaggcac gtacatgcag
    19261 ggcaaactct gagagatctt gagaaattaa cgtcaccatc ccggtatcat gtggacgagg
    19321 ggagacctca ctgaaaatca cctcatcacc acagacaaac agctcgacac caaacagacc
    19381 ataaccgccc agtgccagca ccactttacg ggcaatctcc tgcgcacgtt caagggcaag
    19441 cgggctcatt tgctgtggtt gccaggattc acggtagtcg ccatcttcct ggcgatgacc
    19501 tactggtgca cagaaatgga cgccatccac cgcgctgacg gttagcaggg taatttcgaa
    19561 gtcaaactta acgacgcctt caacaattac gcggcccgct ccggcgcgac cgccttgctg
    19621 agcgtattcc catgcctgag caagttgctc tgcagagcga ataaacgtct gccccttgcc
    19681 ggaagagctc atcaccggtt ttacaatgca gggatagcca atggcggcaa ccgcctcgcg
    19741 gaaaaggctt ttgctatcgg caaaacgata agtggaagtg ggcagctgca gctcttctgc
    19801 cgccaggcga cggataccct cgcgattcat cgttaatttc gttgcgcgag cgcaggggac
    19861 aacattcagt ccttcctctt caagttggat cagcatatcg gtggcaatag cttcgatctc
    19921 cggcacgata taatgtggtt tttccagttc aaccacacgg cgtaatgcat caccatcaag
    19981 catattaatg acatgggagc gatgcgcgac atgcatggct ggtgcgtcgg catagcgatc
    20041 gacggcaatc acctctacgc cgagacgctg acactcgatt gccacttctt tacccagttc
    20101 accggagcct aataacatca cgcgagttgc tgccggacgc agcgcagtgc ctaataacgt
    20161 cataacgaat atcccctgat taattccgcg cgcagtataa acgaaaacgt ttgcgtgtgt
    20221 ctttattgct gatgttgatt tctcaaccga aaagaaatat actgtataaa atctcagtta
    20281 ttatgagagg ctgcaaatgg cggttgaagt taaatacgta gtcattcgtg agggcgaaga
    20341 gaaaatgtcg tttaccagca aaaaggaagc cgatgcttat gacaaaatgc ttgatacggc
    20401 ggatctgctt gacacctagc tgacaaattc tccagtgcaa atggaagacg agcaacgtga
    20461 agccctttcg ctatggctgg cagaacaaaa agaggtgctg agcaccattc tgaaaaccgg
    20521 caaattaccg tccccacagg tcgtcggtgc tgaatccgaa gaggaagatg cctcacacgc
    20581 ggcctgaagc aacttgcgct cctgacaatt tgtaccatgc tttctcctga atcattgtgc
    20641 tatggagaaa aacatgaaaa aaagaggggc gtttttaggg cggttgttgg tttctgcctg
    20701 cgcatcagtt ttcgctgcca ataatgaaac cagcaagtcg gtcactttcc caaagtgtga
    20761 aggtctggat gctgccggaa ttgccgcgag cgtaaaacgt gattatcaac aaaatcgcgt
    20821 ggcgcgctgg gcagatgatc aaaaaattgt cggtcaggcc gatcccgtgg cctgggtcag
    20881 tttgcaggac attcagggta aagatgataa atggtcagta ccgctaaccg tgcgtggtaa
    20941 aagtgccgat attcattacc aggtctgcgt ggactgcaaa gcgggaatgg cggaatatca
    21001 gcggcgttaa tggcttctga taattcgatt gaccgttggc ggatgcggcg cgagtgcctt
    21061 gtccgtccca gaacgcacca caatcgtagg cctgagaaga cgcaacagcg tcgcatcggg
    21121 caatcagcat caaacaatgc cactcaaaac gacaaactac tttcacctaa aataccctta
    21181 tcacctgata tacagtaaat accttacctg gcgcgaattt ctttgaataa cggcctaccg
    21241 ccccgcttat ttgcgcatct tttgtctcga tatacatttt ctgacatcct catccgctat
    21301 gctacaggca ctaaaaatgg ttattgagga gcaaatatgg ctaactggtt aaatcaactg
    21361 caatccctgc ttgggcaaag cagttcttct acctcctcgt ctgcggatca gggattgggc
    21421 aaactgttag tgtcaggcgc attaggcgga ctggctgggc tgctggtcgc aaataaatca
    21481 gcacgtaaac ttcttacgaa atatggcacc aacgcgttac tggttggcgg cggagcggta
    21541 gcgggtacgg tgctgtggaa taaatacaaa gataaaattc gcgcggcgca tcaggacgaa
    21601 ccgcagtttg gcgcgcaaag tacgccgctg gatgagcgta cggaacgttt gatccttgcg
    21661 ctggtctttg ccgctaaaag tgatggtcat attgatgcca atgaacgtgc ggcaatcgac
    21721 cagcaattgc gtgaagccgg cgtggaagag caggggcgtg tactcattga gcaggcaatc
    21781 gaacaaccgc tggatccaca acgcctggct accggggtcc gcaatgaaga agaggcgctg
    21841 gaaatctatt tcctgagctg cgcggctatt gatattgacc attttatgga acgcagttac
    21901 ctgaatgcac tgggtgacgc gctgaaaatc ccccaggatg ttcgtgaagg cattgaacgc
    21961 gatctcgaac agcaaaaacg cactttggcg gaataattcc gctctctgga tagcctgaag
    22021 ttgactattt cgggctatcc agaaccgcac agccatttga acaggctgat tttctctgtc
    22081 tgcataacat tcgatatccg cgccattacc tgtcatcatc taagcaatga cgaccctgtt
    22141 tcgcttgcat ccccggtgag ttttgccacc cttataagat gtttccaacc agaaagaaca
    22201 ataacatgct accaaaagcc gcccgcattc cccacgccat gacgcttcat ggcgatacgc
    22261 gcatcgataa ttactactgg ctgcgggacg atacgcgttc tcagccaaaa gtcctggact
    22321 acctgcaaca agaaaatagt tacggtcatc gggtgatggc ctcacaacaa gccttgcagg
    22381 atcgcatctt aaaggaaatc atcgaccgca ttccgcaaag agaagtttct gcgccctaca
    22441 taaaaaatgg ctaccgctat cggcatattt atgaaccagg ctgtgaatat gctatctacc
    22501 agcgtcaatc ggcattcagc gaagagtggg atgagtggga aacattgctc gatgccaaca
    22561 agcgcgcagc tcatagtgag ttttattcga tgggcggaat ggcgattacg cccgataaca
    22621 ccattatggc gctggcagaa gattttcttt cccgacgcca gtacggcatt cgttttcgta
    22681 atctggaaac aggtaactgg tacccggaac tgctggataa cgttgaaccc agctttgtct
    22741 gggcaaatga ctcctggact ttctactatg ttcgcaagca tccggtgacg ctgctgcctt
    22801 atcaggtctg gcgtcacgcc atcggtacgc cagcatcgca agatacactg atctacgaag
    22861 aaaaagacga tacctattat gtcagcctgc ataaaacgac gtcgaagcac tatgtcgtca
    22921 ttcatctggc cagcgccacc accagtgaag ttcgcctgct ggacgcggaa atggccgatg
    22981 ccgagccgtt tgtttttctg ccgcgccgca aagatcacga atacagcctt gatcactacc
    23041 agcatcgttt ttatctgcgt tccaatcgcc acggcaaaaa ctttggctta taccgtaccc
    23101 gtatgcgtga tgagcaacag tgggaagagt taattccgcc acgcgaaaac atcatgctgg
    23161 aagggtttac gctgtttacc gactggctgg tggttgaaga gcgtcagcgc gggttaacca
    23221 gtttgcgcca aattaaccgc aagacccggg aagtcattgg cattgccttt gatgatccgg
    23281 cctatgtgac ctggattgcc tacaatccag aacctgaaac cgcgcgattg cgttatggtt
    23341 attcttccat gaccacacca gacactttgt ttgaactgga tatggatacc ggtgagcgtc
    23401 gtgtattaaa acaaacagaa gtgcctggtt ttgatgcggc gaattaccgc agtgaacacc
    23461 tgtggatagt cgcccgtgat ggcgtcgaag ttccggtttc gctggtctat catcgcaaac
    23521 attttcgcaa aggacacaac ccgttgctgg tgtatggcta tggttcttac ggcgcaagta
    23581 ttgatgccga tttcagtttt agccgcttga gtttgttaga tcgtggcttt gtctacgcca
    23641 ttgtccatgt tcgcggcggt ggtgagctgg ggcaacaatg gtacgaagac ggtaaatttc
    23701 tgaagaagaa aaatacgttt aatgattatc ttgatgcctg cgatgcattg ttaaaactgg
    23761 gctatggctc tccttcgctc tgttatgcga tgggcggaag tgcggggggc atgttgatgg
    23821 gcgttgcaat taatcaacgc ccggaattat tccacggcgt tatcgcccag gtaccgtttg
    23881 ttgatgttgt aacaacgatg cttgatgaat caattcctct taccactggt gagtttgaag
    23941 agtggggaaa tccgcaggat ccgcaatatt acgagtacat gaaaagctac agcccgtatg
    24001 acaacgtcac cgcacaggct tatccgcatt tactggtaac gaccggttta cacgattctc
    24061 aggtgcaata ttgggaaccg gcaaaatggg tcgctaaatt gcgcgagctg aaaaccgatg
    24121 accatctttt attgctctgt accgacatgg actcaggcca tggcggtaaa tctggccgct
    24181 ttaaatcgta cgaaggcgta gcgatggaat atgcttttct ggtcgcgctg gcgcagggaa
    24241 cattacccgc tacgcctgcg gattaagtat tttccagata atgtttcagt gttaaacgca
    24301 gctccgggct catgctgtcc aggttattaa ataaccagcg cagatagccc ggatagcgtt
    24361 cggcaacgtc ggaaaccgct ttgccacggt atttgccaaa ggtgaaggtt gtcatcaacg
    24421 acggacgtcc ggtgatatcg gccatctgtt ctgccgtcca gccggaggtg ttcataatat
    24481 cgataagcaa cgcggcggtg atataacagt catacaacgc gcggtgatga tgcaggccca
    24541 gcggtgtctg tacattgagc ttgcgtgttt tatataacgc catattgctg tacttgagcc
    24601 ctggccacaa acgacgggcc agtttcatag tgcaaatcca ctcaccgggc atctcaggca
    24661 gtacgcggcg gtcaaagctg gcgttgtgcg cgacatacca ttcactaccg taatagtgtg
    24721 ggatcacatc ttcaatccac ggtttatcgg cgaccatggc ttcggtgatg cgatgaatcg
    24781 ccatcgcttg tggactaata ggacgatcgg ggcgcaccag gaggctcatg gggttgacga
    24841 tttttccgtc aatgacatca acagaggcaa tctcaacgat ccctccctgc aaaccgcagg
    24901 tttctgtatc gataatgcgc aacatggcat gatccaggcc gaaaaagcct agcgtaatgg
    24961 aatgatatcg ccttgccaac cctgtgatga acgctgaccg actaacaata atgaaccacg
    25021 atcggcgcga acgatgagac gaccatattc atcccatagt gcactgttac cacgggcatt
    25081 tgccatcagc acggcaatcg agtactgatg tgaaaaaaat tgtaagcgac gggccgacgc
    25141 cagcaggtcg ggttctccca gacattgccc tgtcgtaaac agtgaacagg tcgggtccat
    25201 atccatacct tgcggttgtt catcgaccac cgtgatggtt ctggaacgac ggcccagaca
    25261 tgcaccatga ctctgatggt agttcccagg cgtttttcgc cacggtgcga acaccgcaat
    25321 tccacgaata aagcgatcgt tatattcaac gggaaggccg gcaatgatgg tcattcgcta
    25381 ggtcgttgcg gcataacaaa gcgggtctaa cagtgaaaga tcgggtgggg caggaagggc
    25441 acgtcgcgaa taatcacacc ccagtaaaga aagcgatgga aaaactaaca gctgacattg
    25501 ttgcctggcg gcggccctca caaactccag atgatgagct acctgttcgg tgagtgaggt
    25561 tttacgaggc tcatattgcg ctgctgcaac tttccagaac gacataatga cttccttttc
    25621 aaaatgactg caaattatgg gggcggttta gaaaatactc ataatttagc aggcgttatg
    25681 taagaaagtg taactctgta taagttttat ttaagtttgg gctcgtaagg taaacgtgac
    25741 agattgaccg aagccaaacg gtgggcaata agccgctcgc gaaaccagct gcgcaaatgt
    25801 tctggctgtt cacgttcaac cgcttcagcg atcaccggca tattgtagcg ttctttaaaa
    25861 gccaccccgg ccgccgccaa atcgacattc actttatcca tttctgtttg atccagttta
    25921 gccagattct tcagcattct taatctcctc atcattcgcg ggcaaaagtt actaagagtg
    25981 agcctggatg gcaagccgga attgatgatc ctcgactcag acattagaca aggttatgct
    26041 ctgtaaaaaa gttattacaa aaaggaatag ataatatggc ttcaactgca cgctcccttc
    26101 gctacgcgct ggcaatcctg accacttcgt tggtgacacc ttccgtctgg gcacatgcgc
    26161 atttaacgca tcagtatcct gcggc
//
LOCUS       DAZPFP010000038        25659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPFP010000038 DAZPFP010000000
VERSION     DAZPFP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.038"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUR4_001563"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUR4_001563"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1455193.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUR4_001564"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUR4_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1455194.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHUR4_001565"
     CDS             2630..3391
                     /locus_tag="ACHUR4_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1455195.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHUR4_001566"
     CDS             complement(3442..4326)
                     /locus_tag="ACHUR4_001566"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1455196.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUR4_001567"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUR4_001567"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1455197.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUR4_001568"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUR4_001568"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1455198.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHUR4_001569"
     CDS             6202..6474
                     /locus_tag="ACHUR4_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1455199.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHUR4_001570"
     CDS             6844..7920
                     /locus_tag="ACHUR4_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1455200.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHUR4_001571"
     CDS             7947..9326
                     /locus_tag="ACHUR4_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455201.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHUR4_001572"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHUR4_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHUR4_001573"
     CDS             10409..11245
                     /locus_tag="ACHUR4_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR1455202.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHUR4_001574"
     CDS             11250..12197
                     /locus_tag="ACHUR4_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1455203.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUR4_001575"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUR4_001575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR1455204.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHUR4_001576"
     CDS             13324..14082
                     /locus_tag="ACHUR4_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1455205.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHUR4_001577"
     CDS             14200..15558
                     /locus_tag="ACHUR4_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455206.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUR4_001578"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUR4_001578"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1455207.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUR4_001579"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUR4_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1455208.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUR4_001580"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUR4_001580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1455209.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHUR4_001581"
     CDS             19503..20054
                     /locus_tag="ACHUR4_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1455210.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHUR4_001582"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHUR4_001582"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1455211.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHUR4_001583"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHUR4_001583"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1455212.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHUR4_001584"
     CDS             23307..24347
                     /locus_tag="ACHUR4_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1455213.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUR4_001585"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUR4_001585"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1455214.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZPFP010000039        24959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPFP010000039 DAZPFP010000000
VERSION     DAZPFP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.039"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            167..1270
                     /gene="proB"
                     /locus_tag="ACHUR4_001586"
     CDS             167..1270
                     /gene="proB"
                     /locus_tag="ACHUR4_001586"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1455215.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1282..2535
                     /gene="proA"
                     /locus_tag="ACHUR4_001587"
     CDS             1282..2535
                     /gene="proA"
                     /locus_tag="ACHUR4_001587"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1455216.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2650..2725
                     /locus_tag="ACHUR4_001588"
     tRNA            2650..2725
                     /locus_tag="ACHUR4_001588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Thr,seq:cgt)
     gene            complement(2740..3894)
                     /locus_tag="ACHUR4_001589"
     CDS             complement(2740..3894)
                     /locus_tag="ACHUR4_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1455217.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3777..4124)
                     /locus_tag="ACHUR4_001590"
     CDS             complement(3777..4124)
                     /locus_tag="ACHUR4_001590"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1455218.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(4211..4555)
                     /locus_tag="ACHUR4_001591"
     CDS             complement(4211..4555)
                     /locus_tag="ACHUR4_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455219.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4656..4835)
                     /locus_tag="ACHUR4_001592"
     CDS             complement(4656..4835)
                     /locus_tag="ACHUR4_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR1455220.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4932..5861)
                     /locus_tag="ACHUR4_001593"
     CDS             complement(4932..5861)
                     /locus_tag="ACHUR4_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1455221.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5864..6055)
                     /locus_tag="ACHUR4_001594"
     CDS             complement(5864..6055)
                     /locus_tag="ACHUR4_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455222.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6057..6686)
                     /locus_tag="ACHUR4_001595"
     CDS             complement(6057..6686)
                     /locus_tag="ACHUR4_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1455223.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6683..7258)
                     /locus_tag="ACHUR4_001596"
     CDS             complement(6683..7258)
                     /locus_tag="ACHUR4_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR1455224.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7255..7419)
                     /locus_tag="ACHUR4_001597"
     CDS             complement(7255..7419)
                     /locus_tag="ACHUR4_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR1455225.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7430..7723)
                     /locus_tag="ACHUR4_001598"
     CDS             complement(7430..7723)
                     /locus_tag="ACHUR4_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR1455226.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7747..8130)
                     /locus_tag="ACHUR4_001599"
     CDS             complement(7747..8130)
                     /locus_tag="ACHUR4_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455227.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8130..8735)
                     /locus_tag="ACHUR4_001600"
     CDS             complement(8130..8735)
                     /locus_tag="ACHUR4_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR1455228.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8837..8995)
                     /locus_tag="ACHUR4_001601"
     CDS             complement(8837..8995)
                     /locus_tag="ACHUR4_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455229.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTETHVH
                     AESSLVSP"
     gene            complement(8992..9144)
                     /gene="kil"
                     /locus_tag="ACHUR4_001602"
     CDS             complement(8992..9144)
                     /gene="kil"
                     /locus_tag="ACHUR4_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR1455230.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(9129..9263)
                     /locus_tag="ACHUR4_001603"
     CDS             complement(9129..9263)
                     /locus_tag="ACHUR4_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455231.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(9458..9928)
                     /locus_tag="ACHUR4_001604"
     CDS             complement(9458..9928)
                     /locus_tag="ACHUR4_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455232.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9937..10263)
                     /locus_tag="ACHUR4_001605"
     CDS             complement(9937..10263)
                     /locus_tag="ACHUR4_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR1455233.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10744..10938)
                     /locus_tag="ACHUR4_001606"
     CDS             complement(10744..10938)
                     /locus_tag="ACHUR4_001606"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455234.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(11028..11660)
                     /locus_tag="ACHUR4_001607"
     CDS             complement(11028..11660)
                     /locus_tag="ACHUR4_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR1455235.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11764..11979
                     /locus_tag="ACHUR4_001608"
     CDS             11764..11979
                     /locus_tag="ACHUR4_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1455236.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            12096..12377
                     /locus_tag="ACHUR4_001609"
     CDS             12096..12377
                     /locus_tag="ACHUR4_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR1455237.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            12412..12573
                     /locus_tag="ACHUR4_001610"
     CDS             12412..12573
                     /locus_tag="ACHUR4_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455238.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12560..13390
                     /locus_tag="ACHUR4_001611"
     CDS             12560..13390
                     /locus_tag="ACHUR4_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR1455239.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            13387..14763
                     /locus_tag="ACHUR4_001612"
     CDS             13387..14763
                     /locus_tag="ACHUR4_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1455240.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14836..15162
                     /locus_tag="ACHUR4_001613"
     CDS             14836..15162
                     /locus_tag="ACHUR4_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455241.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            15159..15359
                     /locus_tag="ACHUR4_001614"
     CDS             15159..15359
                     /locus_tag="ACHUR4_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455242.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            15371..15559
                     /locus_tag="ACHUR4_001615"
     CDS             15371..15559
                     /locus_tag="ACHUR4_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455243.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15562..15726
                     /locus_tag="ACHUR4_001616"
     CDS             15562..15726
                     /locus_tag="ACHUR4_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455244.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15707..16147
                     /locus_tag="ACHUR4_001617"
     CDS             15707..16147
                     /locus_tag="ACHUR4_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR1455245.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            16144..16734
                     /locus_tag="ACHUR4_001618"
     CDS             16144..16734
                     /locus_tag="ACHUR4_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR1455246.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16731..16904
                     /gene="ninD"
                     /locus_tag="ACHUR4_001619"
     CDS             16731..16904
                     /gene="ninD"
                     /locus_tag="ACHUR4_001619"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR1455247.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16871..17046
                     /locus_tag="ACHUR4_001620"
                     /pseudo
     CDS             16871..17046
                     /locus_tag="ACHUR4_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            17049..17450
                     /locus_tag="ACHUR4_001621"
     CDS             17049..17450
                     /locus_tag="ACHUR4_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455248.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            17410..17640
                     /locus_tag="ACHUR4_001622"
     CDS             17410..17640
                     /locus_tag="ACHUR4_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR1455249.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17612..18223
                     /locus_tag="ACHUR4_001623"
     CDS             17612..18223
                     /locus_tag="ACHUR4_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR1455250.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            18220..18426
                     /locus_tag="ACHUR4_001624"
     CDS             18220..18426
                     /locus_tag="ACHUR4_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR1455251.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            18404..19069
                     /locus_tag="ACHUR4_001625"
     CDS             18404..19069
                     /locus_tag="ACHUR4_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1455252.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            19066..19689
                     /locus_tag="ACHUR4_001626"
     CDS             19066..19689
                     /locus_tag="ACHUR4_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1455253.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            20122..20445
                     /locus_tag="ACHUR4_001627"
     CDS             20122..20445
                     /locus_tag="ACHUR4_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR1455254.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            20429..20905
                     /locus_tag="ACHUR4_001628"
     CDS             20429..20905
                     /locus_tag="ACHUR4_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR1455255.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20902..21339
                     /locus_tag="ACHUR4_001629"
     CDS             20902..21339
                     /locus_tag="ACHUR4_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1455256.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21544..22086
                     /locus_tag="ACHUR4_001630"
     CDS             21544..22086
                     /locus_tag="ACHUR4_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1455257.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            22306..22521
                     /locus_tag="ACHUR4_001631"
     CDS             22306..22521
                     /locus_tag="ACHUR4_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1455258.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22669..22911
                     /locus_tag="ACHUR4_001632"
     CDS             22669..22911
                     /locus_tag="ACHUR4_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR1455259.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22947..23435
                     /locus_tag="ACHUR4_001633"
     CDS             22947..23435
                     /locus_tag="ACHUR4_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1455260.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            23413..24912
                     /locus_tag="ACHUR4_001634"
     CDS             23413..24912
                     /locus_tag="ACHUR4_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1455261.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
BASE COUNT         6677 a         5878 c         6109 g         6295 t
ORIGIN      
        1 ggtaatttat taaatctgta ataaaagcgt aaacaactgc cgctacgctt gctgatcccg
       61 cgcaacaaaa cgccatgctt tgctcgcaga tggttggcaa ccgacgacag tcctgctaaa
      121 acgttcgttt gatatcattt ttcctaaaat tgaatggcag agaatcatga gtgacagcca
      181 gacgctggtg gtaaaactcg gcaccagtgt gctaacaggc ggatcgcgcc gtctgaaccg
      241 tgcccatatc gttgaacttg ttcgccagtg cgcgcagtta catgccgccg ggcatcggat
      301 tgttattgtg acgtcgggcg cgatcgccgc cggacgtgag cacctgggtt acccggaact
      361 gccagcgact atcgcctcga aacaactgct ggcggcggta gggcagagtc gactgattca
      421 actgtgggaa cagctgtttt cgatttatgg cattcacgtc gggcaaatgc tgctgacccg
      481 tgctgatatg gaagaccgtg aacgcttcct gaacgcccgc gacaccctgc gtgcgttgct
      541 cgataacaat atcgttccgg taatcaatga gaacgatgct gtcgctacgg cagagattaa
      601 ggtcggcgat aacgataacc tttctgcgct ggcggcgatt ctggcgggtg ccgataaact
      661 gttgctgctg accgatcaaa aaggtttgta taccgctgat ccgcgcagaa atccgcaggc
      721 agaactgatt aaagatgttt acggcattga tgacgcactg cgcgcgattg ccggtgacag
      781 cgtttcaggc ctcggaactg gcggcatgag tactaaattg caggccgctg acgtggcttg
      841 ccgtgcgggt atcgacacca ttattgccgc gggcagcaag ccgggcgtta ttggtgatgt
      901 gatggaaggc atttccgtcg gtacgctgtt ccatgcccag gcgactccgc ttgaaaaccg
      961 taaacgctgg attttcggtg cgccgccggc gggtgaaatc acggtagatg aaggggcaac
     1021 tgccgccatt ctggaacgcg gcagctccct gttgccgaaa ggcattaaaa gcgtgactgg
     1081 caatttctcg cgtggtgaag tcatccgcat ttgcaacctc gaaggtcgcg atatcgccca
     1141 cggtgtcagt cgttacaaca gcgatgcatt acgccgtatt gccggacacc actcgcaaga
     1201 aattgatgca atactgggat atgaatacgg cccggttgcc gttcaccgtg atgacatgat
     1261 tacccgttaa ggagctggcc gatgctggaa caaatgggca ttgccgcgaa gcaagcctcg
     1321 tataaattag cgcaactctc cagccgcgaa aaaaatcgcg tgctggaaaa aatcgccgat
     1381 gaactggaag cacaaagcga aatcatcctc aacgctaacg cccaggatgt tgctgacgcg
     1441 cgagccaatg gccttagcga agcgatgctt gaccgtctgg cactgacgcc cgcacggctg
     1501 aaaggcattg ccgacgatgt acgtcaggtg tgcaacctcg ccgatccggt ggggcaggta
     1561 atcgatggcg gcgtactgga cagcggcctg cgtcttgagc gtcgtcgcgt accgctgggg
     1621 gttattggcg tgatttatga agcgcgcccg aacgtgacgg ttgatgtcgc ttcgctgtgc
     1681 ctgaaaaccg gtaatgcggt gatcctgcgt ggtggtaaag aaacctgtcg cactaacgcg
     1741 gcaacggtgg cggtgattca ggacgccctg aaatcctgtg gcttaccggc gggtgccgtg
     1801 caggcgattg ataatcctga ccgtgcgctg gtcagtgaaa tgctgcgtat ggataaatac
     1861 atcgacatgc tgatcccgcg tggtggcgct ggtttgcata aactgtgccg tgaacagtcg
     1921 acaatcccgg tgatcacagg tggtataggc gtatgccata tttacgttga tgaaagtgca
     1981 gagatcgctg aagcattaaa agtgatcgtc aacgcgaaaa ctcagcgtcc gagcacatgt
     2041 aatacggttg aaacgttgct ggtgaataaa aacatcgccg atagcttcct gcccgcatta
     2101 agcaaacaaa tggcggaaag cggcgtgaca ttacacgcag atgcagctgc gctgacacag
     2161 ttgcaggcag gccctgcgaa ggtggtggcg gttaaagccg aagagtatga cgatgagttt
     2221 ctgtcattag atttgaacgt caaaatcgtc agcgatcttg acgatgccat cgcccatatt
     2281 cgtgaacacg gcacacaaca ctccgatgcg atcctgaccc gcgatatgcg caacgcccag
     2341 cgttttgtta acgaagtgga ttcgtccgct gtttacgtta acgcctctac gcgttttacc
     2401 gacggcggcc agtttggtct gggtgcggaa gtggcggtaa gcacacaaaa actccacgcg
     2461 cgtggcccaa tggggctgga agcactgacc acttacaagt ggatcggcat tggtgattac
     2521 accattcgtg cgtaaataaa accgggtgat gcaaaagtag ccatttgatt cacaaggcca
     2581 ttgacgcatc gcccggttag ttttaacctt gtccaccgtg attcacgttc gtgaacatgt
     2641 cctttcaggg ccgatatagc tcagttggta gagcagcgca ttcgtaatgc gaaggtcgta
     2701 ggttcgactc ctattatcgg caccatttaa atcaataagt tacctcgcat ttaagtaaac
     2761 cacgttctcc tcttgtgccg tatttgtgcc attgcgactt ataatcgcat cgattttgct
     2821 tgcgtgctcg gtgagatgcc cggctgaaag gtgggcgtat ctttgaacca tttcgagagt
     2881 ttcccatcct cccatctctt taagtgcaag aagagagaca ccggactgaa ccagccagct
     2941 tgcccaggta tgcctcagat catggaagcg gaagttgcta atgcctgccc gctttaacgc
     3001 tcccttccat gccttgttgc tgtcggttct catcttcctt accgctgctg tttttgttcc
     3061 gtcgcttcgg taggcaggtt tggtgtggac aaatacccat ctcttatgga gcccctgctg
     3121 ttttcttaat atctggcatg cggtttcgtt aagaggaact ccgatcgcat tgccagcttt
     3181 tgtttcatca gggtgcatcc atgccatttt cttatccaga tcgacctgtg accactcaag
     3241 gtctgtaacg ttggaacggc gaaggcctgt cgtgattgca aacatgacca cagggaagaa
     3301 gtgaggagca atttctgcaa acaggcgctt cgattcctcc tctgtaagcc atctgatgcg
     3361 tccattctta acgcgtggtg ttgatatttt gggtgccctg tcaagccatc cccattcaac
     3421 agccatattg agaatggcgc gaagtattgc cagatgcctc gtcttcgttc ctttgcttgc
     3481 aagctttggt ttatactccg gcaccggatt gccaagtcgc aaacacctgt cccggctcat
     3541 ctcccagttc aggcgatggc ggcggttttc catcccgtct accgcctcca ttattttttc
     3601 tgttgttatg tcagagagaa tggcttctct gaagtgcaac atccagaacg atataatgct
     3661 cttgtcatca tcaatggact tcttatccga tttctcacgc agccaccgta tgcaggcttc
     3721 cttgaatagc tttttcggtg attccccgag attttttact ctccacgctt ctgctttcag
     3781 acgatcgtga agttcttgcg cttgcctttt gtccgatgtt tcaagagagc gtctaactct
     3841 tgatccatct ggcgcgacga aatcgcagtg ccacgtgcca ccgcgtagtt tgattgacat
     3901 gctttaacct cctgcacatc aaccgcattc accgcgctat tgtgtctcac agacttaagc
     3961 gccgcaatgc agtctgactt gcaaatgcga tatgggcttt taggtttatc tggatttatc
     4021 tttgcggcct gaagtcgtcc acttcgtatc cactgcgtga tagtgccttt gtctaccttc
     4081 agatacgacg ctgcctcttc acgagtgaag atttcttctt ccacctggaa tctccattta
     4141 ttggattggt attattgcgg taggtctgga tatcattgag caatgaacag gcctcatcga
     4201 gtgtgaggcg ttagtccttg cgtagctcgc tgattcttct gtaagtctct ggtgctttgt
     4261 tcccgtacgt cttcatttca gacttcaaca gagcaacgag tgaatcccat tcgttgagga
     4321 ttcctttgaa tgccggaacg cgctttgcaa ccttgtcgaa tgaatctctg atttctggaa
     4381 tctgctcaac aagtgcaacg catcgccgga aatcggctgc gtcatgtgga gcaccgaagc
     4441 tatgaccata gatattcttt ttcagtccac atgcgattga ggcaagagtt gcgctactga
     4501 tgccgacatc gtcagtcgat tgccatttca aaaccttcat agccaaatct gacatttctt
     4561 gtctccaata aaaaaccgcc atcaggcggc ttggtgttct ttcaattctt caattcgaat
     4621 attggttaca ttgttttcat atatgaataa ataaattagc ttttttcgtt gccttcgcgt
     4681 tctttattaa ttttgacaaa ctcgttttta ccacgctctc caaatgcgtc tttagagtcg
     4741 ttgtatccgc aatcgcagca cacataatca ccagaccatc cacgcattgt tttttctttt
     4801 gcaatatttc cagaaccgca ttttggacaa gacatatcac tacctccaaa gcatgagtga
     4861 gatgacaacg taacattgat tggagattaa caatagattg ctgatgtaaa agatatgtat
     4921 aagcttcgct ttcaaagtgg aggctctggt agcggcatcc agtgggttac attgctaatc
     4981 agaccatatt cattagtttg aggatggttg ccgttatcgt ctccgtattt aagactctcc
     5041 ataaaaccat aatgcttatt cccattaacg ctcacaaagc cgtaataagc tggtataacg
     5101 ccgatctcac acgtaatcag caaaggaaaa ctagttctcc aatttagctc gccgattaca
     5161 ggcattctct cactacagct tatccaacca tccggagtta ccggaacttg cggaatggct
     5221 gtctgctctc gaacgtcatt aggcgcgata ggttctgctg ccaactgact ggcatatttg
     5281 ttaatggtaa cgataagctc ttgctcagcc tcatccagac aatcaccgat acctcgcctg
     5341 tcaccgtcaa aatcatcgaa atcggcacga atcttggcaa ccttctggat tgcggacaac
     5401 acctcactag gaattaccgg atagttggtt gacgtttccg cgatttcacg aaaattattg
     5461 gttgacgaat tcttgttttc ccgaaagttt ccggactgaa gcatggctgc gcgataggcg
     5521 ttccagccga cagcttttcc gtgttcaaac gcgctgtcaa agtcatcatc catttccatc
     5581 gcatcaggca cagataccgt ctctggaggg gcggcgtaaa gcggagttac ggtaatttcg
     5641 tactcgttta tatcctcgtc gctgatattt gcgaaatagc gaaggatgcc tttctctcca
     5701 cgactacgag cgtcagagtc ctgaatgata tacgccacag gctctgcttc cagcgatgcc
     5761 agcgcaattc gtgccagttc catttgttcg ccacgagtaa gcccgttttc aagcggattt
     5821 ttaatgaaca attcaatacg ttctttggta atagtggtca tagctatttc accttaatct
     5881 caatatttcg cagctttagc tctactggca ggtctgactt tcctgttaac gctaatgcga
     5941 gattttctgg agtaatgaga gcagttattg ttttccccat cgccagacga ataatcagtc
     6001 gtatctcgcg atcgtcacat gctcctggtc gaacaattga tatttgtccg tccatctcac
     6061 tctcctttga tgcgaatgcc agcggcgcgg attgcagcga tgacctcaga aactttgtat
     6121 gccattaccg tttggtaatc atcgtgaaaa tctgttcgat gaagcatgct gctacgttcc
     6181 gggagcagta ttccccgctc ccccagttct gctatgcgat tctctgcgga ttccagcgcc
     6241 gcaaccaatt cgtctacagt tccggcagct tgcagtgcgt aatcggtaat agccatctca
     6301 tgatcaattt cagtaccgtt ctcattcgtt gaggtgatag caaaataatc agagtcgatt
     6361 tcgttatcag ctaagtggcg tagcgtatcg gcaacaagcc ggccgttttc gattagcagc
     6421 ttccctaccg ttagcgcaat atcctcgttc tcctggtcgc gggatttgat gtattgctga
     6481 ttccttaccc gttcatccag tagtgccagc acggtttctg gtccggccag aaatttgaag
     6541 gcgttgagcg catcaatatc cacaccgtaa tctttaagtt cctgttcact taacaaatca
     6601 tcagctggca acattaacag gcgttccatt gctggaattg cacgttccgc cgcctcacgc
     6661 agtgcctgat agttaatttc gctcactggc cgcctccttt gcgaagctgg gcggcgaact
     6721 cgttagccat cgctgaatat gggtgctcat gaccgccaac aaccttcatg ctggcagcaa
     6781 acatctccac gccctgcgcc cgcacttcat ccaggaaagt gtcggtggct ggggtttctg
     6841 gaatggcgtc tggaataacc tctgagtaga cgcgatccat tgccgactcc catccgtagt
     6901 aacaagccga atagccgtcg cgctggtagc cgtggtcttc aactccacat cccatgccct
     6961 ggtcatggta ttcaggctga ttgtcgggat taatgcagta ttcaatgacc gacttcatcc
     7021 ccgcattctc cgccgccagc gccgaaaact tctcgtgtgc caacttaaca gccgcatcag
     7081 cctgcttaat tgactcaatc gctttctggt ggtcttcgta cagagccgaa atcttggcct
     7141 ccgcttcagc aaatttacgc accagatatt cagcgtttgt ttcgttaacc tttaaatcac
     7201 atgggatgca tttacctttc agaaaaccat ccatctcaat tagtgacatt tgtttcattt
     7261 cttcccactc cgccacatag cattcagata tttgttgtca ttaacagaac cgaaactctt
     7321 tctcttaagt aattcctctc tcgatggcat tggctttacg cgttggcgaa taatcatttc
     7381 tgccggaaga atgccgggat tgtatgcaag tcctctcatg gtaaattcct cagtcattac
     7441 tgatagcgcc atagcgtgag cggtaattac gcaggcgcgg gtcaatttca gggaagtggg
     7501 tatatgtggc tttgcggaat ggtcggattg atgtctggta aattcgctcg agttcttctt
     7561 tctctgcaag ccatatacag tggcgaaatt ccttttcctc tttcgtttcc tgcggtagcg
     7621 acattatccg atcgtagttt tttctgaatt tatccagcac ctccgatacg gaattgccgg
     7681 aacagcggcg tgggtcatcc gcaccataca gaggcgctgg cataatggaa tccttatgtt
     7741 gctactttag aagggaattg aatcgtcgta ttcaggatga ttttgatgat tgctactttg
     7801 ctgctgttgg ctgtttcctg aagttgcaaa tccaatcttt gcattcagta attcaagagt
     7861 gattgattgt ccattttgcc cctgataaac atcaaccctg atgttttctc cggtaatttc
     7921 cacaatgcca ccttcaacaa gaacactacg gtagtaatcc gcttgcgctc ccggcttggc
     7981 aaatacaacg gcgctgtagt ttgtccattc tttctttttt gtctggcgat cgtaatactg
     8041 aacgccagca cggatgttga atccgatatt ttccccggcc tgaaactctc ttgcgggctt
     8101 gtttagtctt acagtaatcg aatgtgccat taagcagccg ctccttctaa ttcgtctcgt
     8161 ctgatgttgt aaatgtcctg cgctttgtgc tgctccggtg tgccttcgag catcttccac
     8221 gctttggcga acgcctgttt aagctcttcc acggtgtttt tctgcaatgc tgcgtcagtg
     8281 aatgctttta gaacctgttc aggtgtaggt gatggttttg attgctttgc tgctgcgttc
     8341 tgctgatgtt tatgctcgtc ggtatctgca tctttcgcat catcaatgcc gaacaaacca
     8401 ttgaggcaat acttgcgtgc ataagagctt gtggctcccg taacttgtgc agaatccatt
     8461 cctttcttgc tttcttcctc tcgtgcaaga gcggttgccg tatgactgtt ttcgccatcg
     8521 gtaatagttg ccgtggcttt cacgtaatac cgatcaccaa tcaacacaac ttcatcgctg
     8581 attgataaaa acagaccatt cagtaacggc ttaacgcctt caagaatgtc ttcgcagctt
     8641 ctgtatttat atttaccgaa tgagttgtac tggttctttg gcgcgttcaa gttctcctga
     8701 atagctgcca gtcttgcgta aaattctttg ctcatatgtt tgttctcaga atgggcatgg
     8761 cccaaggaaa taacgctgat ttaatacttc gactcgggac aaattaaggc atacccgcat
     8821 tccttcgcgg tcgccattat ggcgatacca gagagctttc tgcgtgtaca tgcgtctctg
     8881 taacttgctc tccttcactg tggttgcaag tgacatgaat atctccttcg ttaccgatta
     8941 attctttcat ctgacgaatg aattcttcgt ctgaccagtt atctgtaaaa ctcatttcct
     9001 gcgataccac ggaatattga tagctgattt catcgcttta tttgcttcaa gccacatttt
     9061 tgaatcacca ataaatctgg ctattactgc tttgttttgt gctgcacgaa gcatctggtg
     9121 attgatggct atttcattgc gcataacgcc tccagttgtt tctttgctgc actgattaat
     9181 tgtttaactc ggcgtgataa ttcagattcg tgcgggtaga aagcggacat gacgccgcta
     9241 cccgcgagct gaaagtgcat catggataac tccttctgtt tgattgcata acgaaaacgc
     9301 ctcgagtgaa gcgttattgg tatgcatata aaaaagccct catactggag ggcaaagaag
     9361 atttcaaata atcagaacaa gtcggctcct gtttagttac gagcgacatt gctccgtgta
     9421 ttcactcgtt ggaatgaata cacagtgctt actcgtacta ataaaatacc caattttctg
     9481 tttcttggtt gtgcccaaag ttatattcaa tatctggtgt tgatgtatca atattcttca
     9541 tcccatcaac aagagttgat acaacagcca aatcctgttt gattctcatt aaatggtatt
     9601 tcttccggcg caataaactt tcaatggcaa gtttcttcgt tgggaatgca aaagatcttt
     9661 ctgcattttt tgctactttc ttaattgcat atctatttct cttttgtttc cattcctgta
     9721 accactgatt tggtgctggt ttaaaattaa caatccaatg cgcaggaacc aaccatgcat
     9781 aatgctctgt ctgatgaaaa gctatatatt gaagtgcgaa tattttgatt ccatcttctt
     9841 caactgtcgc ctggaatctc cagaaaacag gcattccatc atgttcagtt tctgattcag
     9901 gaaaaggtac gctccatgat tttgtcatat ctcacctcaa ataagtggct tgctgcctaa
     9961 tttcattttc tggcgaccaa cacaagtcac acccatttca ctgcgtggct tgctgtacca
    10021 tgtgcgctga ttcttgcgct caatacgttg caggttgctt tcaatctgtt cgtggtattc
    10081 agccagcacc gtaaggtcta tcggattcag tgcgctttct actcgtgatt tcggtttgcg
    10141 attcagcgag agaatagggc ggttaactgg ttttgcgctt accccaacca acaggggatt
    10201 tgctgctttc cattgagcct gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc
    10261 catcttgatt ctcctgtcag ttagctttgg tggtgtggta ggtgggagac ccatttcgac
    10321 ctgcttcggc cgacttcaat tcggcaatag ttccgcaggc ctcgccgctt tacgtgcgac
    10381 atattcccat ccatgaaccc ttcaccacac cccaaagcct tctgctttga atgctgccct
    10441 tcttcagggc ttaattttta agagcctcac cttcatggtg gtcagtgcgt cctgctgatg
    10501 gcttaaaatt acaagaaaga ttgtatgttg taaacaagaa atattgtaaa aaggggcgta
    10561 aaaacaaact ccattgtttt taaatgaaaa atagtttgtt tttggttatc gagattgagg
    10621 tggggattac tggttgcagg ttccgactac atcaccaaca aatgatttgg ttgatgtaag
    10681 ttgttgcata cctgggatat tcattacttt ggagtaaaga gcttttttgt ctgtagtgat
    10741 tgatcaagtt tcaacggtta ttcctcctcc agactggtat tctcctacca tagtgttcga
    10801 tgacaaagca gtgtatttca tctctggata gacgccagaa actgattcat aaactgatga
    10861 tttatcgcca tttattgtta cgtggaaaac ggaatcttcc gtgctgtctt ttgtaaactc
    10921 gtaacgatcg ccattcattg acccgtaccc gtgcaggttt gtgacaatcc agcattcaga
    10981 attggcgctg gtagttaaga gtattgagag tagcgccgca atcctgatca tacgaatttt
    11041 accctcgctt ccacgacaac accgataatc ttgcagttcc cgttgatagg agtcataggc
    11101 catgaagggt tcaggccttt caggtacttc tgcccgccat ctataaccag tttcttgaat
    11161 gttgcttcgt tcgcgtcagt cagtttggct acaacaaggc ttccattcac tggctcgcgt
    11221 ccagtatcta ctaacaccat atgaccttca gggatgcttt gacctacagg tgaggtcatg
    11281 gaatcacctt caaccttcag ccagaatcca tcgcctaata agttaacgtc actgtcatac
    11341 cattcatcaa tgtccttgat atcgtagggt tcacaagctt cacaccacga accagctcta
    11401 accatgctaa tcaatggata tttccctttg ggctcaacat gcccaacaaa tctaacattc
    11461 gaatcagagg tgccattgag cagccagtca acacttacgc caagagctga cgcaagttct
    11521 ggtaaaaagc gtggtcgctt agttttaccg ttttcgagct gctctataga ctgctgggta
    11581 gtccccacct tttgagcaag ttcagcctgg ttaagtccaa gctgaattct tttgcttttt
    11641 accctggaag aaatactcat aagccacctc tgttatttac ctccaatctt cacaagaaaa
    11701 actgtatttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt tgatggaggc
    11761 gatatgcaaa ctctttctga acgcctcaag aagaggcgaa ttgcgttaaa aatgacgcaa
    11821 accgaactgg caaccaaagc cggtgttaaa cagcaatcaa ttcaactgat tgaagctgga
    11881 gtaaccaagc gaccgcgctt cttgtttgag attgctatgg cgcttaactg tgatccggtt
    11941 tggttacagt acggaactaa acgcggtaaa gccgcttaag acattcccgc tcttacacat
    12001 cccagccctg aaaaagggca ttaccagaaa caaatctcta tggttttgcg tttctttgcg
    12061 aagccaactc tatctaatca ttaaggaaat tatctatggg tactattgca actaaaagca
    12121 agaaagcggc tcgcatcgag tcagccttgc tgaacaaact ggcactgatg gggcagaaga
    12181 cattcgctcg agcaatgggg gttcctgaat atcaggtaag ccgatggaag aatggtttct
    12241 tctcgcaggt aagcatgatg cttgctgttc tggaatacgg aatcgaagac gatgaaatgg
    12301 ctgaattgac taagcggctt gccgattacc tgacaaaaga aaaagccccg aagaacggcg
    12361 aattcttcga ggcctaatgt agaaagactg gatcaatcca cgggagtaat tatgacaaaa
    12421 cgtcgtaaga aataccagga aaaagaagag attcgacacc ctgattcacc tgagggatta
    12481 gtggtagccg cagcaaataa cagggcgttc gcagagcgcc ttgttggtgt ttacagacta
    12541 gccaaagcag gagtgaaaca tgggcgtcgt taagttagct gattacaggc ctcaactgga
    12601 ggtcgtggag catcgcgtgg cagaactcga agatggctac actcggactg caaacacact
    12661 gttagaagcc gccatgcttt ctggacttac tctacatcag ttactgattg ttatggctgt
    12721 gtggcgcaag acatacggtt acaacaaaaa aatagattgg atcggaaacg aacagttcgc
    12781 tgaactcact ggcatggcac caaccaaatg ctctaccgcc aaaaacgagc ttatcagaat
    12841 gggagttctc attcaggtgg ggcgtcaagt tggtatgaat acaaacattt ccgagtggaa
    12901 aacgaaagtt aacggattcg gtaaaacatt taccaattcg gtaaaacaaa ccttcaccaa
    12961 atcggtaaaa agcaatttac cgaatcagtc aaacacaaaa gacaatatac aaaagacaat
    13021 aaatacaaat acccccttac cccctaacgg gggcggcgat gggcaggtta aacctgaacg
    13081 tcgcaaggca gaacgaatcg actacgaatc cttcctgaac gcctacaaca ccgaagtcgg
    13141 tgacagactg ccacacgctg ttgcggtcaa cgagaaacgc aaacgccgcc tgaagaaaat
    13201 catcccgcaa ctgaaaacgc caaacgtgga cggtttcaga gcgtatgtca gggcgtttgt
    13261 gcatcaggcc aagccgtttt acttcggaga caacgacacg ggctggacgg ccgattttga
    13321 ttacctgctg agggaagact cgttaacggg ggtacgggaa gggaagtttg cagacagggg
    13381 gattgcatga aacaggatat cgaagcgagc gttatcggtg gcctgctgat tggtggatta
    13441 acaccaaccg ccagcgacgt tctggcaacg ctggagccgg aagcgttttc aattccgctc
    13501 taccggaaag ccttcgaggt tatccgcaag caggcgcgaa acagaaacct aatcgacgcg
    13561 ctgatggttg ccgaggcgtg cggagaggag catttcacgt caatcctgat gaccagcaag
    13621 aactgcccga gtgccgcaaa cctgaaggga tatgctggaa tggtcgcgga taactatcac
    13681 cgccgtctgg tgctggaaat catggatgaa atgcgtgaac caatccaaag cggaaccatc
    13741 gacgcatcga gtcaggcgat ggatgaactt gtaaagcgtc tttcagccat cagaaagccc
    13801 cgtgacgagg taaaacctgt acggttaggg gaaatcatca ctgactacac tgacacgctt
    13861 gacaggcgtc tgaggaacgg agaagagtca gataccctga agaccggaat cgacgaactt
    13921 gacgccatca ccggagggat gaacgcagaa gacctggtga ttatcgccgc tcgtcctggt
    13981 atgggtaaaa ccgaactggc gctgaagatt gccgaaggcg ttgcaagccg cgttattcct
    14041 ggttctgacg tccggcgcgg agtgttgatt ttctcgatgg aaatgagcgc attgcagatt
    14101 gcagagcgga gcattgccaa cgccgggagg atgtcggtta gcgtgctgcg aaatcctgca
    14161 tcgatggatg acgaaggctg ggcgcgtgtt gctaacggca tgagtcagct tgcagatttg
    14221 gatgtatggg tagtcgatgc ctcgcggtta tcggtcgaag aaatacgctc aatcgcagaa
    14281 cggcacaaac aggaaaatcc aaacctgtca ctcatcatgg cggattatct tggcctgatt
    14341 gagaagccga aagcagaccg caacgacctc gcaattgctc acatctccgg aagcctgaag
    14401 gcgatggcga aagacctgaa aacgccagtt atctccctga gtcagctttc gcgcgatgtt
    14461 gagaagcgac caaacaaacg cccgacaaac gcagatttgc gtgattcagg aagcattgaa
    14521 caggacgcag actcaatcat catgctctat cgggaagcgg tatatgacga gaacagtagc
    14581 gccgcgccat ttgctgaaat catcgtgacg aaaaaccgtt ttggctcgct tggtacggtt
    14641 taccagcggt tctgtaacgg acactttgtt gcatgtgacc aggatgaagc cagacagatt
    14701 tgcacagcat caaatgcacc tgctgcgcgt ggcagacgat atgcacaagg ggctgacgta
    14761 tgaccatcta catcactgag ataataacag gcctgctggt aatcgcaggc ctttttattt
    14821 gggggagagg gaagtgtggc tgactggcaa attccaatca tcattcttgc cggagcttcg
    14881 ctggttgctg gctttatcct gctgaaaaag cataaagacc gtgatcaaaa agttgaagtt
    14941 ctctatgggt atccagcgaa cagcacaaca tggctgacca tttaccacta ccgaaaatca
    15001 ggacgctggg tattcgaatg ggatgatctg ttcgctgaaa agcgaccaaa gtcatgggga
    15061 gacatcagcg aatgcatgat gtttgaagaa agaaaatccg gcgcaacccg agaagagttt
    15121 aacgaagcgt gggcgcgatt aagtgagaga gggtatttgt gagcaagtac gaaaaattag
    15181 atcaaaacat tctttcaatg ctgagtgaaa gaccaacacc tgtttttgat atctggctta
    15241 aatggcggag caatggaatg tatatcgaaa ccatcgatcg ccgtatgcaa tacctgagaa
    15301 agaaagggct tgttgcaaat gtgcgtggga agggttgggt gaaaattaac ctgtcataac
    15361 ggggattgat atggacgata gcttagaaat atttgaagag tggtgcgctg atgaacttgg
    15421 tgtctccgct tacttcattc gacaaatgcg aagcaagaat attttgggcg tgattgagta
    15481 taaacgagtt gaaataaaca agagatacag ggcttggatg gccgcagttc gcgccgccgg
    15541 agtcaaagtg aaggagtgag tatgagcgca tacgaagaaa tcatgttagc cctgcgattc
    15601 tttttcggtg tggaagaaga tgaaaacgta aatgagatta tcggacaaga ccatgacccg
    15661 atagggacta ttgcagctgc acttgacgat tacaggagcg taaatggtga ggaaactaac
    15721 gtttgaacta agaagcccca tccatcagca gaacgccatt caagctatcc agcaaatcct
    15781 tccagaccca accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca
    15841 gaatcgaaag ctttgggctt gccttggtga cgtttcgcgt caggttgaat ggcatggtcg
    15901 ctggctggat gcagaaagct ggaagtgtgt gtttaccgca gcattaaagc agcaggacgt
    15961 tgttcctaac cttgccggga atggatttgt ggtaataggc cggtcaacca gcaggatgcg
    16021 tgtaggcgaa tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt
    16081 taaatggtca gacgaagcgc gactggctct ggagtggaaa gcgagatggg gagataaaac
    16141 tgaatgaaca aataccgact tatttacgca gatccgcctt ggcaatatcg cgacaaagcc
    16201 aacgatggca atcgcggtgc tggacataaa tacgatgtta tgaatgttca ggacatttgc
    16261 cgactgccag tatgggattt agcggatcca gaatcttgct tgttagcgat gtggtgggtg
    16321 ccgacacagc cagccgaagc gctaaaggta attgaggcgt ggggattcag gttgatgact
    16381 atgaaaggct ttacttggca caaaaccaat aagcacaaag gcaacagtgc gatcggaatg
    16441 gggcatatga ccagggcaaa tagcgaggat tgcttgtttg ccgttcgagg gcggttacct
    16501 gagagaatgg acgcttcaat atgccagcac tttaccgcac cgagaatgga gcacagtgca
    16561 aaaccaccga tcgtaagaga catgttagct aagttgcttg gcgacgtgcc gcgctgtgag
    16621 ttatttagcc gcgacaaagt gaccgggtgg gatatgtggg gaaatcagtg cgactccgat
    16681 tttgaacttg ctcccggcat ggcgattaaa ccatgcaaaa tggtgatcgc atgaagcact
    16741 gttatcgatg tggagagcga aaggaagacg atcgctttcg accaggacag ccttactgga
    16801 atcgatggtg tctccggtgt gaaagaacac caacaggggt gttaccacta ccgcaggaaa
    16861 aggaggacgt gtggcgagac agcgacgaag tatcaccgac ataatctgtg aaactgcaaa
    16921 taccttccaa cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga atctgacgta
    16981 aaaaccttca actacacggc tcacctgtgg gatatccggt ggctaagaca tcgtgcgagg
    17041 aaatgacaat gcttttaatt caacctggat ttggacttag catcaaaaaa ggccacatgt
    17101 ttggcgagaa agagtcacaa cgaaaaatga tgtctatccg gttgccattt atcagtattt
    17161 attggctaaa cagggaggca acaaattatt ggtatacatg cgccagagca gcatttaacg
    17221 accctgactg gtttgtggaa aaccaccacg cagttcgtca ggcaaagaga aaggccaaca
    17281 tgacatacat gaaggcgtat cgaaaagcat ggaaagaaca ccgcgatcga taccagcaag
    17341 atatggaaaa gcttgaatca gaaaacatgg aattaagacg aaagcttggt gaagcaaaac
    17401 gagacattga tgcttacaag cgacttttta atggtgaaag ccatgcttag tccatcccaa
    17461 tctcttcaat accagaaaga aagcgtcgag cgggctttaa cgtgcgctaa ctgcggtcag
    17521 aagctgcatg tgctggaagt tcacgtgtgt gagcactgct gcgcagaact gatgagcgat
    17581 ccgaatagtt caatgtacga ggaagaagac gatggccaaa ccagcgcgaa gacgatgtaa
    17641 aaacgaagaa tgtcgggaat ggtttcaccc tgcattcgct aatcagtggt ggtgctctcc
    17701 agagtgtgga accaagatag cactcgaacg acgaagtaaa gaacgcgaaa aagcggaaaa
    17761 agcagcagag aagaaacgac gacgagagga gcagaaacag aaagataaac ttaagattcg
    17821 aaaactcgcc ttaaagcccc gcagttactg gattaaacaa gcccaacaag ccgtaaacgc
    17881 cttcatcaga gaaagagacc gcgacttacc atgtatctcg tgcggaacgc tcacgtctgc
    17941 tcagtgggat gccggacatt accggacaac tgctgcggca cctcaactcc gatttgatga
    18001 acgcaatatt cacaagcaat gcgtggtgtg caaccagcac aaaagcggaa atctcgttcc
    18061 gtatcgcgtc gaactgatta accgcatcgg gcaggaagca gtagacgaaa tcgaatcaaa
    18121 ccataaccgc catcgctgga ctgtcgtaga gtgcaaggcg atcaaggcag agtaccaaca
    18181 gaaactcaaa gacctgcgaa atagcagaag tgaggccgca tgacgttctc agtaaaaacc
    18241 attccagaca tgctcgttga agcatacgga aaccagacag aagtagcacg cagactgaaa
    18301 tgtagtcgcg gtacggtcag aaaatacgtt gatgataaag acgggaaaat gcacgccatc
    18361 gtcaacgacg ttctcatggt tcatcgcgga tggagtgaaa gagatgcgct attacgaaaa
    18421 aattgatggc agcaaatacc gaaatatttg ggtagttggc gatctgcacg gatgctacac
    18481 gaacctgatg aaaaaactgg agacgatagg attcgacacc aaaaaagacc tgcttatctc
    18541 ggttggcgat ttggtcgatc gcggtacaga gaacgtcgaa tgcctggaat taatcacatt
    18601 cccctggttc agagctgtac gtggaaacca tgagcaaatg atgattgatg gcttatcaga
    18661 gcgtggaaac gtcaatcact ggctacttaa tggcggcggc tggttcttta atatcgatta
    18721 cgacaaagaa attctggcta aagctctttc ccataaagca gatgaacttc cgttaatcat
    18781 cgaactggtg agcaaagata aaaaatatgt catctgccac gccgattatc cttgtgacga
    18841 atacgagttt ggaaagccag ttgatcatca gcaggtaatc tggaaccgcg aacgaatcag
    18901 caactcacaa gacgggatcg tgaaagaaat caaaggcgcg gacacgttca tctttggtca
    18961 tacgccagca gtgaaaccac tcaaatttgc caaccagatg tatatcgata ccggcgcagt
    19021 gttctgcgga aacctcacat tgattcaggt acagggagaa ggcgcatgag actcgaaagc
    19081 gtagctaaat ttcattcgcc aaaaagcccg atgatgagtg actcaccacg ggccacggct
    19141 tctgactctc tttccggtac tgatgtgatg gctgctatgg ggatggcgca atcacaagcc
    19201 ggattcggaa tggctgcatt ctgcggtaag catgaactca gccagaacga caaacaaaag
    19261 gctatcaact atctgatgca atttgcacac aaggtatcgg ggaaataccg tggtgtggca
    19321 aagctcgaag gaaatactaa ggcaaaggta ctgcaagtgc tcgcaacatt cgcttatgcg
    19381 gattattgcc gtagtgccgc gacgcctggc gcaagatgca gagattgcca cggtacaggt
    19441 cgtgcggttg atatagccaa aacagagcag tgggggagag ttgtcgagaa agagtgcgga
    19501 agatgcaaag gcgtcggcta ttcaaggatg ccagcaagcg cagcatatcg cgctgtaacg
    19561 atgctaatcc caaaccttac ccaacccacc tggtcacgca ctgttaagcc gctgtatgac
    19621 gctctggtgg tgcaatgcca caaggaagag tcaatcgcag acaacatttt gaatgcggtc
    19681 acacgttagc agcatgattg ccacggatgg caacatatta acggcatgat attgacttat
    19741 tgaataaaat tgggtaaatt tgactcaacg atggataaat gcactcgtta aataaagccc
    19801 tgagttaata gctcggggct ttttgcgttt taagcacggc ctttctgaaa gcacatcaag
    19861 ccaaatacca gacagacaaa aataatcacc ttatccgctt ggctacggtg cggtgtgctt
    19921 tgcataaaag aaaaccagcg caatggctgg cttcgtgaaa gcgggtggca agaggctgcg
    19981 ctaacaacct catgccgttt tgcccgtgca tatcggtcac gaacaaatct gattactaaa
    20041 cacagtagcc tggatttgtt ctatcagtaa tcgaccttat tcctaattaa atagagcaaa
    20101 tccccttatt gggggtaaga catgaagatg ccagaaaaac atgacctgtt agccgccatt
    20161 ctcgcggcaa aggaacaagg catcggggca atccttgcgt ttgcaatggc gtaccttcgc
    20221 ggcagatata atggcggtgc gtttacaaaa acagtaatcg acgcaacgat gtgcgccatt
    20281 atcgcctggt tcattcgtga ccttctcgac ttcgccggac taagtagcaa tctcgcttat
    20341 ataacgagcg tgttcatcgg ctacatcggt actgactcga ttggttcgct tatcaaacgc
    20401 ttcgctgcta aaaaagccgg agtagaagat ggtggaaatc aataatcaac gtaaggcgtt
    20461 cctcgatatg ctggcgtggt cagagggaac agataacgga cgacagaaaa ccagaaatca
    20521 tggttatgac gtcattgtag gcggagagct atttactgat tactccgatc accctcgcaa
    20581 acttgtcacg ctaaacccaa aactcaaatc aacagcagcc gggcgctatc agcttctttc
    20641 ccgttggtgg gatgcctacc gtaagcagct tggcctgaaa gacttctctc cgaaaagcca
    20701 ggacgctgtg gcattgcaac agattaaaga gcgtggcgct ttgccgatga ttgatcgcgg
    20761 tgatatccgt caggcaatcg accgttgcag caatatctgg gcttcgttgc ccggggctgg
    20821 ttatggccag ttcgagcata aggctgacag cctgattgca aaattcaaag aagcaggcgg
    20881 aacggtcaga gagattgagg tatgagcaga gtaaccgcga ttatctccgc tctggttatc
    20941 tgcatcatcg tctgcctgtc atgggctgtt aatcattacc gtgataacgc catcgcctac
    21001 aaagagcagc gcgacaaagc cgcatccact atcgctgata tgcagaagcg tcaacgtgac
    21061 gtagcagaac tcgacgccag atatacaaag gagcttgctg atgctaacgc gactatcgaa
    21121 agcctccgtg ctgatgtttc tgctggtcgt aagcgcctgc aagtcgccgc cacctgtgca
    21181 aagtcaacga ccggagccag cagcatgggc gatggagaaa gcccaagact tacagcagat
    21241 gctgaactca attattaccg tctccgaagc ggaatcgaca ggataaccgc gcaggttaac
    21301 tacctgcagg aatacatcag gactcagtgc ctgaaataat ttttttgcaa atcacaaagt
    21361 ccatttaatg agcctcgcga tgcggggctt ttttacatct gaatttcaca gcgcatctca
    21421 cgcgcatatt acatcacccg agcctttcag aaagttgagc ctgagaactg ccgtatatgg
    21481 tggcgaccat ctcgggacgg cttttctgtg agacaggctc actttctaaa aggtaaagac
    21541 gcaatgaact acccaaccgt tgttaacgat atagatttca gagacctaat ttttgtagca
    21601 aacaacgatc cggttacaga ttcttttatg gtggcaaaag catttggaaa gctgccgaag
    21661 aacgtagttc gtgacattga gcgaaccata gaagcttgcc ctcctgagtt tgatacaaag
    21721 ctcaactttg agctttgcta taaaaacaat gagttacaga atggtaagcc gcaaaaattc
    21781 taccgtctcc gcaaggatgg gttgatgctt ttggttatgt ccgacaccaa aaaagaagca
    21841 atgcgtatca aaattgctta catcaacgca tttaactgga tgtacgccat gcttcaggtt
    21901 ggtcatcgtc aatttgaaga agagagaaat gccgtaatgc tggagtacat gaaagagaag
    21961 gatgttgcca gcatgtcagg tcgcctgcta aatcgctggg gcaaaattaa gaagccacag
    22021 ctgatggcta gaattgaacg ccttgaacag cacgggcaaa ccgtaatccc cggactcatc
    22081 aattaacggc agcaccgcga aacaacccaa gccagtaagt ggggaaataa cactggcagc
    22141 cactgaaaga tgaacctcca gccttatggc aaaaaagatt ctttgtggtg gcggactgat
    22201 ggaaagacat cggatagaat caagcagtgg ctagggtagc tcccgaaaag cggactcgtc
    22261 accgcctgcc actgaatcta tggcgaacaa ctagacgagg ttgtgatgga tgataacgga
    22321 aaccaatggg tcaatgttga acaccggcta ccagaaagca aagaagggat gtggtctaaa
    22381 gaggttatag ctcttactga tactggtgac gtgttcaaac tatcatgtat gggctcttac
    22441 tggcagagaa ccaaggcatt catcgattca ggagcaagca aggttacaca ctggatgccg
    22501 cttaattacc cagatgatta aaacggatga aaaatgaaag gtcgctcagg cggccttttt
    22561 tattgccatc acaaaagcca ttccttactg agtggctttg ataatggctt ataccctaca
    22621 cgggataact taactgatat cccttttaac ggataaatgg agccaacaat ggcagagatt
    22681 attcccatga ctgaagaaca gaaattccag ttagagattt acaaactggt catgaaccag
    22741 aacgcagccg cagaagaagc atttcaattc attggcactg acgaactgaa gcttgagcta
    22801 ttcaaaattc acttccagtc aggtggcgct aattcagata tcacgatccg cacatttgaa
    22861 gcggtgcgta aatcgaagga agcgttagac ctgttcacca ccggagcatg atgctcaacc
    22921 tgaaataacg attaagtgag atgaatatgg cagcaccaaa gggcaaccga ttctgggagg
    22981 cccgcagtag tcatgggcga aaccctaaat tcgaatcgcc tgaggcgctg tgggctgctt
    23041 gttgtgaata cttcgagtgg gctgatgata acccgctatg ggagggtaag gtattttcat
    23101 atcagggaga aataattaag gctaatgtcc ctaagatgcg agccatgact atttcaggat
    23161 tgtgtacctt ccttgatatc accaggcaaa catggggaac cttccggtca atggaaggtt
    23221 tttctgacgt cacatcacga gcggaagaca tcatctacga ccagaaattc tctggcgcag
    23281 ccgctgacct tctcaacgct aacatcatcg cccgtgattt gggcctcaaa gagcagtcgc
    23341 aagttgaaga cgtgacacct gataagggag atcgcgataa gcggcgctct cgtatcaagg
    23401 agctattcaa ccgtggaact ggacgcgatt cttgataact tgagcgacga agagcaaatc
    23461 gaattgctcg agctactcga agaagaagag aactaccgta acacacacct gctatatgaa
    23521 tttacgccat acagcaaaca gcgtgagttc atcgacgccg ggcatgacta tccagagcgc
    23581 tgttttatgg ctggtaacca gcttggtaag tcatttactg gtgctgctga agtcgcgttt
    23641 caccttaccg ggcgttatcc gggcacaaaa ggctatcctg ctgatggtaa atatggcggt
    23701 gagtggaaag gtaagcgttt ttatgagcct gttgtctttt ggattggtgg cgagacaaac
    23761 gagactgtaa ccaaaacgac tcaacgcatc ctgtgtggtc gtatcgaaga gaatggtgag
    23821 ccaggctacg gttccatacc taaagaagac atcattagct ggaagaagtc tcctttcttt
    23881 ccgaaccttg ttgatcacct tctggttaag catcacacgg ctgatggcgt tgaagatggc
    23941 atttcaatct gctacttcaa gccatactcg caaggtcgtg ctcgctggca gggtgacaca
    24001 atccacggcg tgtggtttga cgaagagcca ccatacagca tttatggtga aggccttacc
    24061 cgtaccaaca aatacgggca attctcaatt ctgacgttta ccccgctgat ggggatgtct
    24121 gacgttgtta ccaagttcct gaagaatcca agcaagtcgc agaaagtggt caacatgacc
    24181 atctacgacg ctgagcacta cacagacgaa cagaaagagc aaatcatcgc atcttatcct
    24241 gagcatgaga gagaggcgcg tgctcgcggt attcctacga tgggtagtgg tcgaatcttc
    24301 cagataccgg aagagacgat taagtgtcag ccgttcgagt gtcctgatca cttctacgta
    24361 attggcggga tggatttcgg atgggatcac ccgcaggcgc aggttcagct ttggtgggac
    24421 aaggacgcag acacaatata cgtttcacgc gtgtggaagg cgaaagaaaa aacagccgtt
    24481 caggcgtggg gagccgttaa atcatgggcg cataaagtgc caactgcatg gcctcatgac
    24541 ggaaaccagc acgagaaggg cggcggtgag cagctcaaag ggcagtatgc agatgctggt
    24601 tttatgatgt tgcaggagca tgcgacatgg cctgatggcg gtaatgctgt ggagcctggc
    24661 atcactgaat tgcgcgacat gatgctcgat ggtcgcttca aagtattcaa cacctgtgag
    24721 ccattctttg aggagttccg cctctatcac cgtgatgaaa acgggaaaat cgtcaagctt
    24781 aacgacgacg ttctctcagc cgttcgctat gcatacatga tgcgccgctt cgccaaaatg
    24841 atgcgcgaca tcaaaaaacc aaaagagaaa aagataccag ccccaatcag gcccatcgca
    24901 cggagaactt aaatggccga cgaaaacaga ctcaattcca ttctgtgtaa gtttgacgc
//
LOCUS       DAZPFP010000040        24606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPFP010000040 DAZPFP010000000
VERSION     DAZPFP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.040"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..806)
                     /locus_tag="ACHUR4_001635"
     CDS             complement(<1..806)
                     /locus_tag="ACHUR4_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1455262.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIRLIILFLLAA
                     LSFIGLFFMINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNT
                     EEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGR
                     VIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKI
                     HVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGN"
     gene            complement(906..1817)
                     /gene="gsiD"
                     /locus_tag="ACHUR4_001636"
     CDS             complement(906..1817)
                     /gene="gsiD"
                     /locus_tag="ACHUR4_001636"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1455263.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1820..2740)
                     /gene="gsiC"
                     /locus_tag="ACHUR4_001637"
     CDS             complement(1820..2740)
                     /gene="gsiC"
                     /locus_tag="ACHUR4_001637"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1455264.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2758..4296)
                     /gene="gsiB"
                     /locus_tag="ACHUR4_001638"
     CDS             complement(2758..4296)
                     /gene="gsiB"
                     /locus_tag="ACHUR4_001638"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1455265.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4316..6187)
                     /gene="gsiA"
                     /locus_tag="ACHUR4_001639"
     CDS             complement(4316..6187)
                     /gene="gsiA"
                     /locus_tag="ACHUR4_001639"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1455266.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6174..7139)
                     /gene="iaaA"
                     /locus_tag="ACHUR4_001640"
     CDS             complement(6174..7139)
                     /gene="iaaA"
                     /locus_tag="ACHUR4_001640"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1455267.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7343..8578
                     /gene="moeA"
                     /locus_tag="ACHUR4_001641"
     CDS             7343..8578
                     /gene="moeA"
                     /locus_tag="ACHUR4_001641"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1455268.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            8578..9327
                     /gene="moeB"
                     /locus_tag="ACHUR4_001642"
     CDS             8578..9327
                     /gene="moeB"
                     /locus_tag="ACHUR4_001642"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1455269.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9506..10168)
                     /gene="fsa"
                     /locus_tag="ACHUR4_001643"
     CDS             complement(9506..10168)
                     /gene="fsa"
                     /locus_tag="ACHUR4_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1455270.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            10299..11198
                     /locus_tag="ACHUR4_001644"
     CDS             10299..11198
                     /locus_tag="ACHUR4_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1455271.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11204..13636
                     /locus_tag="ACHUR4_001645"
     CDS             11204..13636
                     /locus_tag="ACHUR4_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1455272.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13782..14597
                     /gene="ybiV"
                     /locus_tag="ACHUR4_001646"
     CDS             13782..14597
                     /gene="ybiV"
                     /locus_tag="ACHUR4_001646"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1455273.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14749..16014
                     /locus_tag="ACHUR4_001647"
     CDS             14749..16014
                     /locus_tag="ACHUR4_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1455274.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16255..17847)
                     /locus_tag="ACHUR4_001648"
     CDS             complement(16255..17847)
                     /locus_tag="ACHUR4_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1455275.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18066..18986
                     /gene="ldtB"
                     /locus_tag="ACHUR4_001649"
     CDS             18066..18986
                     /gene="ldtB"
                     /locus_tag="ACHUR4_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1455276.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19044..20162)
                     /locus_tag="ACHUR4_001650"
     CDS             complement(19044..20162)
                     /locus_tag="ACHUR4_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1455277.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20159..20626)
                     /gene="mntR"
                     /locus_tag="ACHUR4_001651"
     CDS             complement(20159..20626)
                     /gene="mntR"
                     /locus_tag="ACHUR4_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1455278.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20812..20940
                     /gene="mntS"
                     /locus_tag="ACHUR4_001652"
     CDS             20812..20940
                     /gene="mntS"
                     /locus_tag="ACHUR4_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1455279.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21212..22795
                     /gene="opgE"
                     /locus_tag="ACHUR4_001653"
     CDS             21212..22795
                     /gene="opgE"
                     /locus_tag="ACHUR4_001653"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1455280.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22843..23358)
                     /gene="ompX"
                     /locus_tag="ACHUR4_001654"
     CDS             complement(22843..23358)
                     /gene="ompX"
                     /locus_tag="ACHUR4_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1455281.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23411..23476)
                     /locus_tag="ACHUR4_001655"
     CDS             complement(23411..23476)
                     /locus_tag="ACHUR4_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455282.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23711..24598
                     /gene="rhtA"
                     /locus_tag="ACHUR4_001656"
     CDS             23711..24598
                     /gene="rhtA"
                     /locus_tag="ACHUR4_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1455283.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         6036 a         6665 c         6088 g         5817 t
ORIGIN      
        1 taattacctt cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg
       61 atctcatgac cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac
      121 tcgttcagat accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga
      181 tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa
      241 ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc
      301 gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact
      361 tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag ataatattta
      421 ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc
      481 acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg
      541 gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg
      601 ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatcat gaaaaaaaga
      661 ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt ttcgtataaa
      721 ctcagcatgt taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata
      781 gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac
      841 aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa
      901 cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg
      961 aacgccagta cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata
     1021 accatatccg ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca
     1081 ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg
     1141 aaaaacacca cgatagaaga gacggtccca ggcaggatat gacgcaataa aatggtcata
     1201 tcgctggcac caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg
     1261 ttgccgcgca ccaggcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg
     1321 ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag
     1381 gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca
     1441 gccaacaagc ccagcaacgt ccctatcgcc acaccgataa acacggcaaa cacgcccgcc
     1501 gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg
     1561 acgccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt
     1621 tcggcgtcat agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg
     1681 aataatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac
     1741 ggtgtacgta cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc
     1801 cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca
     1861 catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa
     1921 tcaccgggta atcgcgcatt tctacagagt caacgagtaa gcgtccaagt ccgggccagt
     1981 tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca
     2041 tggtcactac cgggatcatc gcgttgcgta gcccgtgttt gaggacaacc caggtttcgc
     2101 tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacgtcg acaaatgacg
     2161 cacgggtaaa gcgcgccatc acggcggcca ctgccgcgcc gagcgtcagg gagggtaaaa
     2221 tgtagtgctg ccaactgtct gctcccacag taggcagcca gcccagttca acggagaata
     2281 cctgaattaa aaacatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg
     2341 tcatactcaa tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca
     2401 taccaaatat caccgcccag accatactgg ttatggtcag ccatagcgtt ggcataaagc
     2461 ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct
     2521 gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct
     2581 gctgacgcac cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg
     2641 gatcgccggg cagcatgtgg acaaataaaa acaccagcac cgagacgata aacagcgtcg
     2701 gaatcaaccc cagtaatcgt ttgataacgt aataaagcat tccactccct gcgttgctta
     2761 ttgcaaatcc gcgtcttcaa agctgaagcc ggtatctggc atgatccaaa aaccggtcag
     2821 gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgattcttg
     2881 ccagatgata tcctgcgccg ccttatataa gcgggtcttt tccgccggat cattcgtttt
     2941 cagtgcctga gccaggaagt catccacctg tttattgctg taaaacgcgg tattaaacag
     3001 cgtcggtggc cagttttgcg aggcaaacag cggcgatagt gcccagtcgg cttcgccggt
     3061 tgaagccgac cagccagtgt agaacatccg cacgccactc tctttttgcc ctttaccttc
     3121 aacttctgcc gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg
     3181 cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga
     3241 ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt
     3301 cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac
     3361 accagtagct ggagttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat
     3421 ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat
     3481 actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc
     3541 cagcagtgtg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag
     3601 cattgccgcg cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg
     3661 caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca
     3721 ggtatccagt tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt
     3781 ttccagcgct gtcggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc
     3841 tgagaacggc tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc
     3901 aatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag
     3961 attcgctttt accgccactg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg
     4021 caatttcacg gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt
     4081 tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc
     4141 ctgagataac gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc
     4201 caccaccaca tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag
     4261 cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc
     4321 tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt
     4381 gcaacgagac ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg
     4441 acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta
     4501 atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc gcaccgtgaa ccaatttcaa
     4561 caatttgccc gagatacatc accgccacac gatgactaat ccgctctacc acagccatat
     4621 cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga
     4681 taatctgccc gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt
     4741 ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact
     4801 catgcgggta acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa
     4861 cgcgtgcagc cgcgtcttta cctggcaata aaccgtgtac acgcagcggt tcgataatcg
     4921 aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa
     4981 tatcccggcg caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa
     5041 agataatttc gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt
     5101 taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga
     5161 ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga
     5221 aacgggtgac aagattacgc acccgtaaaa caggttcgcc atccaccacc gttttctgct
     5281 cgatgggggg ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat
     5341 aatctaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag
     5401 gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat
     5461 acatcaccag tacccgatcg gcaatctctg ccaccacgcc catatcgtga gtgataaaga
     5521 taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa
     5581 tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca
     5641 gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc gaataacgtg
     5701 aaagaatggt ttgtgcctcc ggaatgcgta cctgatccag catccgcttc gcctcgacca
     5761 tcgcttcttc acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca
     5821 cagtaaatac tgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac
     5881 cgcgcacatg gcgcatttgt gcagcgctct gctcgctaag ttcaatcaca tcacgactgc
     5941 gccgccgcaa cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacagacgca
     6001 tcaatgccag cgcagtcact gacttaccgg agccggattc gcctacaatt gccagcgtct
     6061 caccgcgttg cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca
     6121 taaaggcaat attaagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt
     6181 gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag
     6241 ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggtcg
     6301 atagcgatta agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg
     6361 caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc
     6421 agcgcgcgga tgaagacttc gcctgtgccg gtacaagaaa ccgccacact ggcgttattg
     6481 gcgtagcatc cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc
     6541 attccgcctg tggacgtggc tgccgccaga ttgccgtcta aatccaacgc cacggccccc
     6601 acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gactgttgcc
     6661 ccttcctcgc gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggt
     6721 gagacgcgct ccatgccatg agcaaacgca aaattttctg ccccttcgcc aatcatcatc
     6781 acatgcgggc tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta
     6841 acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca
     6901 tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc
     6961 agcagacgca ccgcttccgt caccacatcc agcgcacttt cgccggcttc cagcattttc
     7021 tgcccggttt caacaatggc agacaacgcc tcgatgtagc gtaattcctg ttgcagactc
     7081 atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata
     7141 gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat
     7201 tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg
     7261 ctacgcccga ggtggcatac agtctatggc gtttttctga cataataggc aaattcgatt
     7321 ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc
     7381 gcttaatgag atgctttctc gcgttacccc actgaccacc caggaaacgc tgccactggt
     7441 tcagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccactggatg tcccggggtt
     7501 tgataactcc gctatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc
     7561 gctgcccgtt gccggtaaat cctttgccgg tcagccatac catggtgaat ggcctgcggg
     7621 tacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat
     7681 gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg
     7741 gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg
     7801 aactcgcctg actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc
     7861 ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg
     7921 tcagccgctg ggcgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt
     7981 agaacagctg ggttgcgagg taatcaactt agggattatc cgcgacgatc cacatgccct
     8041 gcgcgccgca tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt
     8101 ttcagtgggt gaagcggatt acaccaaaat gattcttgaa gagctggggg agatcgcctt
     8161 ctggaagctg gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg
     8221 gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca
     8281 gcctttgctg gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt
     8341 acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct
     8401 gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat
     8461 atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt
     8521 ggacgtgcgc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg
     8581 gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt
     8641 gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tggtagtcgg cctcggtgga
     8701 ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc
     8761 gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag tgatgccacg
     8821 gtcgggcaac cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc
     8881 gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa
     8941 cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc
     9001 tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc
     9061 acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc
     9121 gagaacgcgt taacctgcgt agaagcaggc gtgatggcac cgttgatagg cgtaattggt
     9181 tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg
     9241 aaaatcgtca tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgcaat
     9301 ccggggtgtg aggtgtgcgg gcagtaattg ccacctccaa gcctgataag acgcgacaag
     9361 cgtcgcatca ggcatttgct ccagacgcca gatgcggcgt gaacgcctta tctggcctac
     9421 ggttcgggca cagatttcca agcgcggcag gcatcggtgc actccgccac gtcatcagca
     9481 atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct ccctgccagt
     9541 cctgctcaaa cttcgtcaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca
     9601 catccagtgg cagagtaatt gattcacatc cggccagtaa gcagtccagc gcctgacgcg
     9661 gggttttgaa actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt
     9721 gtaagtcggt cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag
     9781 gcgcaacata ttccgcacct gccagcgccg acagcagccc ttgtgccgcg ccatataccg
     9841 cggttcccag cgtcggaatc ccttccgctt ttaacatctt aatcgctgcc agcccctcgg
     9901 ctgtcaccgg aactttcacc acgatatccg caataataga acgcagctta agcgcgtcat
     9961 tcaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca
    10021 tcgcttcatg aagttgcgga agcacaacat ccagcggttt tttacccgcg gcgataatgc
    10081 ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa
    10141 cgtctgaagt atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat
    10201 gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg
    10261 aaagtatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct
    10321 actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg
    10381 gctgccgctg gtgtcagaac ccggaaagcc gcgcccgtac gcaggatctg ctgtatgacg
    10441 cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc cgcgccggaa gtgattgagc
    10501 gcgcgctgaa tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgt
    10561 taaccgactg ctgtccgaca caggcgttaa ccgtgtgtgg tgaagtgaaa agcgttgagg
    10621 agatcatggc gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggcttaa
    10681 cgctttcggg tggtgaaccc ttcatgcagc cggaaatggc gacggcgctg ctgcaagcct
    10741 gccacgaggc aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata
    10801 tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg
    10861 cgccgtttaa acagtggacc gacggtaacg ccgccagggt gctggataac ctgaaaaaac
    10921 tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg
    10981 acgaaacctc tgtaaaagcc attaccgatt ttgccgccga cgagctgcac gttggcgaaa
    11041 ttcattttct gccctaccac acgctgggca tcaacaaata tcacttactt aatctgccct
    11101 atgacgcccc ggaaaaaccg cttgatgcgc cagagctgct cgattttgcc cagcagtatg
    11161 cctgccagaa agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact
    11221 ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaagcc
    11281 gccagtctgt accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa
    11341 gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc gcaccatctg
    11401 gatcaaacac gatgagttga tcattggcaa ccaggcaagc gaagttcgcg ccgcgccgat
    11461 cttcccggaa tatactgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc
    11521 cggtgctggc tttgcggtga gcgaagagaa caaacgtgtt ctgcatgaag tgtgcccgtg
    11581 gtggcgcggt cagacggtac aggatcgctg ctatggcatg tttaccgatg agcaaaaagg
    11641 tctgctggcg accggcatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct
    11701 ggcggtcaat ttcccgctgc tgctggaaaa agggcttgat ggtctgcgtg agaaagtagc
    11761 ggaacgtcgc tcgcgcatca atctgacggt gctggaagat ttgcacggcg aacagttcct
    11821 gaaagcgatt gatatcatgc tggtggcagt cagtgaacac attgaacgtt tcgctgccct
    11881 ggcgcgtgca atggccgcga ccgaaacccg cgaaagccgt cgcgatgaac tgctggcgat
    11941 ggcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca
    12001 actgtgttac ttcatccagt tgattttgca gattgaatct aacggtcact cggtgtcgtt
    12061 tggtcgtatg gatcagtatc tctacccgta ctatcgccgc gacgttgaac tgaaccagac
    12121 gctggatcgc gaacacgcca tcgaattgct gcatagctgc tggttgaaat tgctggaagt
    12181 gaacaaaatc cgctccggct cccactcaaa agcctctgcg ggaagtccgc tgtatcagaa
    12241 cgtcaccatt ggcggacaaa atctggttga tggtcaacca atggacgcgg tgaatccact
    12301 ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt
    12361 gcgttaccat gcaggaatga gtaacgattt cctcgacgcc tgcgtacatg tgatccgctg
    12421 cggcttcggg atgccggcat tcaacaacga cgaaatcgtg atcccggaat ttattaaact
    12481 cggtattgaa ccgcaggacg cttatgacta cgcagcgatt ggttgtatag aaaccgccgt
    12541 cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat
    12601 gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga
    12661 aaaagcgttg tcggcaggta acttcaacaa cttcgatgaa gtgatggacg cgtgggatac
    12721 gcaaatccgt tactacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct
    12781 ggaagagaac gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc
    12841 gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggtcggtat
    12901 tgccaacctc ggcaacagcc tggcggcagt gaagaaactg gtgtttgaac agggcgcgat
    12961 tggtcagcaa cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca
    13021 actgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg ataccgtcga
    13081 tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataaccc
    13141 acgctacggt cgtggtccgg ttggcggcaa ctattacgcg ggtacgtcgt ctatctccgc
    13201 taacgtaccg tttggcgcgc aaactatggc aacgccggac gggcgtaaag cccacacccc
    13261 gctggcagaa ggcgctagtc cggcctctgg tactgaccat ctcggcccta ctgcggtcat
    13321 tggctcagtg ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa
    13381 actgaatccg gcaacgctgg agaacgaatc tgacaagcag aaactgatga tcctgctgcg
    13441 taccttcttt gaagtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac
    13501 gctgctggag gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg
    13561 ttattccgcc ttcttcaccg cgctctctcc agacgctcag gacgatatca tcgcccgtac
    13621 tgaacatatg ctgtaatctc ttcggcccgg ctgcggcgtc gccgggctaa atcattcaca
    13681 tcatcaattt catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta
    13741 ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg
    13801 tcacagatat ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta
    13861 tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc agcggtaatc
    13921 agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg
    13981 aaaacggcgc actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac
    14041 atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct
    14101 gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg
    14161 caaaacacta ccatcgcctg aaacctgtaa aagattatca ggagattgac gacgtactgt
    14221 tcaagttttc gctcaacctg ccggatgaac aaatcccgtt agtgatcgac aaactacaca
    14281 tagcgctgga tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta
    14341 ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac
    14401 cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc
    14461 gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta
    14521 ccgatgataa taatcatgaa ggcgcgctga atgtgattca ggctgtgctg gataacacat
    14581 ccccttttaa cagctgacct ctctaccgga gccatgcgct ccggttttcc cttcttttta
    14641 catcctgtac atcacatcaa atcattaaac ttgcattaac ttatttataa cttgtattgt
    14701 cacatgcaaa ttagcatagt ttcgaatgaa agttaatgca aggtggttat ggcttctact
    14761 tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg
    14821 ctgcgggcgc agcttggcga tgtccagcag atctttaatc agctaagcga tgacattgcc
    14881 acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg
    14941 tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt
    15001 cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag
    15061 tcgatgctgg actatctgaa ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat
    15121 aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag
    15181 gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt
    15241 ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac
    15301 cctgaccgta tccgccgcct tccgcccgga acgacctcca aaggtcttgg agcgcatacc
    15361 gactccgggg cgctggaacg ctggctgctt ccagcgtatc agcacgtttt cgccaacgtc
    15421 tttaatggca atctggcgaa atacgatccc tggcatgcgg cacatcgtac ggaagttgaa
    15481 gagtacacgg tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggact
    15541 gcgctctcgg atatgctgcc tggtcagggg ttgctgcacg tcgtgcccat tcctgaagcc
    15601 atggcgtacg tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc
    15661 gtagcgcccg gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg
    15721 ttaaccagca ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc
    15781 cattccgttg cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg
    15841 gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa
    15901 ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt
    15961 acgcttgccg acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta
    16021 atggaagtga attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca
    16081 agtgcgcttt cttaatgcct gatgcggcgt taacgcctta tccggcctac aaagtcctgc
    16141 agattcaata tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt
    16201 gcatttgtca tcagccgtcg tcaccccgac agtatcgggg tgacggataa tcacttactc
    16261 gatcccttta ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg
    16321 ggtgatttcc agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa
    16381 gatcagcgtg ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc
    16441 caggtggttg gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc
    16501 aaacagcatc cgcccttttt ccccaccgga aagcacttta gctggctttt tgatgtcgtc
    16561 ctggctgaac agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg
    16621 cttccactga ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc
    16681 ctgggcgtag taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg
    16741 cagatcgccc accagcgttt tcagcagcgt tgatttaccg acgccgttgg tacccagtac
    16801 cgcaagtttt tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc
    16861 aaaccctttg gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc
    16921 aaaacggata aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc
    16981 aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct
    17041 aacgaaagat tgcaactcag caatctgcgc tttcttcttg gcgttatcgg ccagcagacg
    17101 ttcacgcgct tgggtcgccg ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag
    17161 ctcgccgtaa tccagatccg ccatgtgggt acagaccatg ttaaggaagt gacggtcgtg
    17221 cgagatgatg atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt
    17281 gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga atatccggct cagcaaacag
    17341 cgcctgcgcc agaagcacac gcagcttcca gccaggagca acttcactca tcgggccgta
    17401 gtgctgttcc actggaattc ccacgccaag caacagttca ccggcgcgag cttccgcaga
    17461 gtaaccgtcc atttcgccgt atttcacttc cagatcggcc actttatagc cgtcttcttc
    17521 actcatttcc ggcaaagcat agatgcggtc gcgctcctgc ttcacttccc acaactcttt
    17581 atgccccatg atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg
    17641 cagcttacca atgcgctcgt tgggatcgag ggaaacgtta cccagcgtcg gctcaaggtc
    17701 gccgccgaga atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta
    17761 acggttgccg ccgccgaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg
    17821 catggtgacg ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc
    17881 gcattatgcc ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc
    17941 aaaggcaaaa aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat
    18001 aatgcgcttc caatactcta atattctcaa cccaatggcc tgccaggcac aaaatctcgc
    18061 ttaacatgaa tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt
    18121 gcagtaccgc ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc
    18181 agaatcaggt gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg
    18241 agtaccagat ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc
    18301 cgaaaggcgg tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg
    18361 aaggcatcgt cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca
    18421 ccgttatcgt gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga
    18481 ccaccaaagt tgagcataag aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg
    18541 cagagtaccg cgctgcgggc gaaccgctac cggctgtcgt tccggcaggt ccggataacc
    18601 cgatgggtct gtatgcactc tacatcggtc gcctgtatgc tatccatggc accaacgcca
    18661 acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca
    18721 aattcctgtt cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa
    18781 aagcgaccac cgagccagac ggcagccgtt atattgaagt ccataacccg ctgtctacca
    18841 ccgaagccca gtttgaaggt caggaaatcg tgccaattac cctgacgaag agcgtgcaga
    18901 cagtgaccgg tcagccagat gttgaccagg ttgttcttga tgaagcgatc aaaaatcgtt
    18961 ccgggatgcc ggttcgcctg aattaatcgt caaaacgtaa aacaaaggcg gactcataat
    19021 ccgccttttt tatttgccag accttaccag gccgggatca taactaacaa aacgtatccc
    19081 accagcgccg cccacaacag catcggtatt gaatagagat ggaaacgcca ccagatgcgg
    19141 cgatcgttcg ccatacgtag cgcaatcaaa tttgccagcg atcctggtaa taaaccaaag
    19201 ccccccacgt ttaccgccca tgccagtaat aaagacggag gtacataatt gagcagcaaa
    19261 atggtactcg gcacgttact gatcacctgc gataaaccga ctgccgttaa ccataacccg
    19321 ggttcagata aatgactcac gttacccaac acgccttgca acgctggaag ctgggtcagt
    19381 aaatggacgt cgataaacat cgccataaac accagcagca acgtccagtc cacactcaac
    19441 accacgcgac gcgccagcag cacaaagccc gccgccacaa tcaccagtcc ccacagctct
    19501 tgtttgaact ccagcgccgt cagaaagacg atatacagcc ccagacaatt ccacaccagc
    19561 cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcgcctt tccagggaaa
    19621 caacaccagc acaacagcag gagtgtcagc atcattgcgc cagccatcgg tgccatttgg
    19681 gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg
    19741 ccaattggcg tcagtagcga accagcgttg actgccagcg cctcaaaaat aatcagccga
    19801 ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc
    19861 gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc
    19921 agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg
    19981 cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat ggtgtgccag
    20041 tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca
    20101 acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa aggcaggctc
    20161 atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct tcactaacat
    20221 ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaaca
    20281 agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg
    20341 ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt gccagccgct
    20401 taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg tccacctgac
    20461 gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct
    20521 cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta
    20581 actgcgtcac tttttttgct attggcgtac ctgcgcgacg actcattgtg cttcctcatt
    20641 atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag
    20701 gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg
    20761 taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag
    20821 ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatgctgctc
    20881 tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc agataaatag
    20941 cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcataacgcg ttttggtctg
    21001 aaaaaaacca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag
    21061 ttgacctgaa cggcggctcg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg
    21121 atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg acgccagggt
    21181 aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt
    21241 tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat
    21301 taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta cggctatttt
    21361 gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct
    21421 ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt
    21481 gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg
    21541 gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa
    21601 aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt
    21661 ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa
    21721 agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt
    21781 gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct ggacggtcac
    21841 tgccgttaag ccgaagtatc agacctatgt ggtagtgatc ggtgaaagcg cgcgtcgcga
    21901 tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg
    21961 tttgatattt gctgactaca ttgctgccag tggctccacg cagaaatcgc ttggcttaac
    22021 gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca ccctggcaaa
    22081 tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac
    22141 tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag aaggtaattt
    22201 tgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc aagtgctggc
    22261 gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc
    22321 ctgcgacagg acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa cgtcgtgcta
    22381 tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa
    22441 cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct ttaaagagcg
    22501 cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc
    22561 ttttatggtc atttccagcg gcgataaagc gcatcgggtg attaaagccc gccgctcagc
    22621 caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg aaataaatat
    22681 caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt
    22741 acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca
    22801 aaaatccgcc ccgagaggcg gatttttata tcaccaaagt gattagaagc ggtaaccaac
    22861 accggcaatc caggtgccta cgtcaacgct acgaatacgg ctctgctcgt aagagaagtc
    22921 cagagcaacg ttttccatcg ggttgaactg cagaccagca ccgtaggaga aaccgtagtc
    22981 gctggtgtcg tgtttgtagg tcgggtattc agtggtctgg aatttaccat aacccacacc
    23041 cactacaccg tagatgcttg cccagtcgtt aatgcggtaa gccggaccag cagtgatgcc
    23101 gtagtactgg tttttgttgt agtcaccaga gcttgcagta cggcttttct cggtgtaagt
    23161 gaaagaaccg atcacaccca gcgggctgtt gtcttcttca tagcggtatt tcaggttgaa
    23221 accgcccatt ttgttcattt ggccctgagc gtcgctctgt gcgtaaccgc cagttacggt
    23281 agaagtcgca gctacggaag tacctgcggt gaaagccaga actgcggcca gtgctgaaag
    23341 acatgcaatt tttttcataa ccacctcaaa tgtgattcaa ataagtccta agttttaaat
    23401 atatcaaaaa ttaatgggaa actcttcgcg atttgtgatg tctaacgggc catttcatgt
    23461 aacagaacgt ttccatacac cgctatccat ctaaatttaa atcacttttt cagagaactg
    23521 cgtaagtatt acgcatgttt tccctgtcat tcatccagat tattcctaat caccagacta
    23581 atgattccat caatcctggc gcattttagt caaaacgggg gaaaattttt tcaacaaatg
    23641 ctcaaccagc attgggtata tccagtacac tccacgcttt acttaagtct agatatttgt
    23701 gggagaaagg atgcctggtt cattacgtaa aatgccggtc tggttaccaa tagtcatatt
    23761 gctcgttgcc atggcgtcta ttcagggtgg agcctcgtta gctaagtcac tttttcctct
    23821 ggtgagcgca ccgggtgtca ctgcgctgcg tctggcatta ggaacgctga tcctcatcgc
    23881 gttctttaag ccatggcgac tgcgctttgc caaagagcaa cggttaccgc tgttgtttta
    23941 cggcgtttcg ctgggtggga tgaattatct tttttatctt tctattcaga cagtaccgct
    24001 gggtattgcg gtggcgctgg agttcaccgg accactggcg gtggcgctgt tctcttctcg
    24061 tcgcccggta gatttcgtct gggttgtgct ggcggttctt ggtctgtggt tcctgctacc
    24121 gctggggcaa gacgtttccc atgtcgattt aaccggctgt gcgctggcac tgggggccgg
    24181 ggcttgttgg gctatttaca ttttaagtgg gcaacgcgca ggagcggaac atggccctgc
    24241 gacggtagca attggttcgt tgattgcagc gttaattttc gtgccaattg gagcgcttca
    24301 ggctggtgaa gcactctggc actggtcggt tattccattg ggtctggctg tcgttattct
    24361 ctcgaccgct ctgccttatt cgctggaaat gattgccctc acccgtttgc caacacggac
    24421 atttggtacg ctgatgagca tggaaccggc gctggctgcc gtttccggga tgattttcct
    24481 cggagaaaca ctgacaccca tacagctgct ggcgctcggc gctatcatcg ccgcttcaat
    24541 ggggtctacg ctgacagtac gcaaagagag caaaataaaa gaattagaca ttaattaaat
    24601 ttacat
//
LOCUS       DAZPFP010000041        24317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPFP010000041 DAZPFP010000000
VERSION     DAZPFP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.041"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHUR4_001657"
     CDS             complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHUR4_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1455284.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHUR4_001658"
     CDS             complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHUR4_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1455285.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2464..2706)
                     /locus_tag="ACHUR4_001659"
     CDS             complement(2464..2706)
                     /locus_tag="ACHUR4_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1455286.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHUR4_001660"
     CDS             complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHUR4_001660"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1455287.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHUR4_001661"
     CDS             complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHUR4_001661"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1455288.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5623..6597)
                     /locus_tag="ACHUR4_001662"
     CDS             complement(5623..6597)
                     /locus_tag="ACHUR4_001662"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1455289.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6749..8689
                     /gene="csrD"
                     /locus_tag="ACHUR4_001663"
     CDS             6749..8689
                     /gene="csrD"
                     /locus_tag="ACHUR4_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1455290.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8994..10037
                     /gene="mreB"
                     /locus_tag="ACHUR4_001664"
     CDS             8994..10037
                     /gene="mreB"
                     /locus_tag="ACHUR4_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1455291.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10103..11206
                     /gene="mreC"
                     /locus_tag="ACHUR4_001665"
     CDS             10103..11206
                     /gene="mreC"
                     /locus_tag="ACHUR4_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1455292.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11206..11694
                     /gene="mreD"
                     /locus_tag="ACHUR4_001666"
     CDS             11206..11694
                     /gene="mreD"
                     /locus_tag="ACHUR4_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1455293.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHUR4_001667"
     CDS             11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHUR4_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1455294.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12286..13755
                     /gene="rng"
                     /locus_tag="ACHUR4_001668"
     CDS             12286..13755
                     /gene="rng"
                     /locus_tag="ACHUR4_001668"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1455295.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHUR4_001669"
     CDS             13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHUR4_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1455296.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17779..19224
                     /gene="tldD"
                     /locus_tag="ACHUR4_001670"
     CDS             17779..19224
                     /gene="tldD"
                     /locus_tag="ACHUR4_001670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1455297.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHUR4_001671"
     CDS             complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHUR4_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1455298.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHUR4_001672"
     CDS             20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHUR4_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1455299.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHUR4_001673"
     CDS             20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHUR4_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1455300.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHUR4_001674"
     CDS             21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHUR4_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1455301.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23672..23944
                     /locus_tag="ACHUR4_001675"
     CDS             23672..23944
                     /locus_tag="ACHUR4_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1455302.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24000..24263)
                     /gene="yhcN"
                     /locus_tag="ACHUR4_001676"
     CDS             complement(24000..24263)
                     /gene="yhcN"
                     /locus_tag="ACHUR4_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1455303.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5759 a         6128 c         6598 g         5832 t
ORIGIN      
        1 taagttctca atttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc
       61 ggcaaattta cgtgcctttt gtgagcttgc tcgcacttcg ccccgcgtca ccctacggcg
      121 atgcgaaggt caattcttac gaccggtaat acggcaccac tcttcttttt ccacgaccgg
      181 gtccagtgcg aagctatcgg cataagcttc acaaacgctc tctgcctggc ttgccagaat
      241 accggaaagg cccagcaaac cgcctgaaac tggcaggacg ctgattaacg gtgccagttc
      301 acgtaatggg cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg
      361 atctttcggt aagtagagtt ccagacggtc agaaacgcca ttacgttcgg cgttatcgcg
      421 gctggcctga atcgcctgcg gatcgatatc aataccaatg gcttttgctg cacccagttt
      481 cagcgccgcg atcgccagaa tgccggaacc acagccaaag tcgatgactg ttttaccggt
      541 taaatcgagg ctgtcgagcc attgcaggca cagagaggtg gttggatggg tacccgtacc
      601 aaacgccagc cctggatcta acatcacgtt gacggcgttt tcgtccggca catcacgcca
      661 gctagggcag atccacagtc gttcaccaaa gcgcatcggg tggaaattat ccatccattc
      721 gcgctcccag tctttatctt ctagttgttc gattttatgc gcgaagcctg cgccgagcag
      781 cggatggttt tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc
      841 aatcacatcg gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa atactggcgt
      901 atcgtgggta tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag
      961 atcttccgcg ttcgcgccgg tggtgttcag tttcagttgg atccaaggca tggcaaaact
     1021 ctttatttat cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg
     1081 accaggaaag ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac
     1141 tgaatattca gcgtcgcgag tacggcatac agcacgccgc caacgatcat cgcacttagc
     1201 gcgcctttgg cgttggcgcg ttcccagtaa agacccagca ccagcggcca caggaaaacg
     1261 gcttccagcc caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag
     1321 gcggcaagca gcagcaacgc gccgagaact aacgtaatta ccgccgacat ccgcttcaga
     1381 cgcgtctcgt tttgcatttg atccggacgg atattcagat agagatcttt aatgatcgta
     1441 gcggaacttt gcagcagttg ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc
     1501 agaaagatcc cggcagcaaa aggtggcagc acttttacca ttaacgtcgg gatcaccaga
     1561 tctggtacgg tgagatcggg gatcactgca cgacctaacg ctccggccag gtgcataccg
     1621 aacatcagga ttgcgactat aatcgtaccg atgatgatcc cccggtgtac tgctttgctg
     1681 tctttataag agatacagcg caccgcagta tgcggcaggc caatcacgcc aaagcacacc
     1741 agtacccaga acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta
     1801 accagttgcg gatcgatggt ttgcaaggtc tgtactgcgt tgcttaagcc gccagcggca
     1861 tgtaccacgc caataagcag cacaacggtg ccaatcagca tcacaagccc ttgcatggtg
     1921 tcgttcagca cgctggcgcg aaagccacca aaggcggtat ataacgcaat gctgatacca
     1981 aaaatcagca gcccggtttc ataaggaata cccgccgcgg tttccagcag gcgcgcaccg
     2041 ccgataaact gcacggtcat cgcaccaacg aacgcaacca gcaaactcaa actcgccagc
     2101 cacaccagaa gacgactctg gtagcgggca aacagcatat cgttcagcgt cactgcattg
     2161 tagcggcgcg caagaatcgc aaacttcttg ccgagaatac cgagtgaaag ccagacagca
     2221 ggaagctgaa tcatcgccag caatacccag cccagcccgt atttataagc agctcctggc
     2281 ccgccgataa acgaactggc actgatatag gtcgcggtga gagtcatcgc cagcacaata
     2341 ccgcccatag agcggctgcc gaggaaatac tcattaagga aggtgccggt gctccgttta
     2401 cgcatcgcat aaaccgagat accgaacacc accaccagat aggcgaccag cggtagaatt
     2461 acttcaagct gcatcgtcat cctccagtgg gatatcgcga tagataaatt tcaccatcgc
     2521 ccagcacagt ccaataaaca gtagcggcgt caggatgcag gccatctcaa accagcgcgg
     2581 aaagccggta aaaccggggg caacgccagg taagtaagcg tctactaacc aaactgccag
     2641 atacaaaagg gtcagcccca gcgcccagcg ggcctcttta tgggcctgaa caaaacgagt
     2701 gtccattttt tgtccctgat gggtgaagaa agcgaggatt gtaccttatg ggggttgtcg
     2761 atccccagta ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt
     2821 ttcctgaaga ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt
     2881 ctcgtcattc atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag
     2941 ctcctgcagc gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat
     3001 cagcttaccg atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga
     3061 ctcccaacgt acgccaaaac cgccaggtgc gtggaaacgg gtgattttgc ctgggcttgg
     3121 caggaaggtg ttcggatctt cggcgttgat acgacattcc accgcatggc cgcgaacgtg
     3181 aacttcttct tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat
     3241 caggtcaacg ccggtgatca tttctgtaac cgggtgttct acctgaatac gggtgttcat
     3301 ttcgatgaaa tagaactcgc cgttttcgaa caggaactcg aaagtacctg caccgcgata
     3361 gccgatatca acacacgctt tagcgcaacg ttcgccgatg tagcgacgca gttccggggt
     3421 aatgcccggt gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc
     3481 acgttccgcc agatagatag cgttgccctg accgtcagcc agtacctgaa tctcgacgtg
     3541 gcgaggattc tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc
     3601 ttccgcacgg gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat
     3661 accgcgaccg ccgccgccgc cggaggcttt gataatcacc ggataaccaa tgcgtttagc
     3721 aatggcgcgg tttttatcca tatcgtcgcc cagcgggccg tcagaaccag gtacgcaagg
     3781 aacgcccgct tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt
     3841 ttctgctttc gggccgatga agataaagcc ggagcgttca acctgctcgg caaagttggc
     3901 gttctcggag aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc
     3961 gctgatgatt gccgggatgt tcagataact ttttactgac ggagcagggc caatacagac
     4021 cgtttcatct gccagtaata cgtgttttag atcgcgatcc gtgctggagt gcacagcgac
     4081 agtcttgatg cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt
     4141 ggcaataaca attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt
     4201 caaattctac cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg
     4261 cttcgatctg gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga
     4321 ctttctgacc cacttcgatg aacgcttttg cgtccgggct tggggtgcgg tagaaagtac
     4381 caaccatcgg ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg
     4441 aaggaacggt cgccggagcg gctgcgttag atttagctgg ctgctgcatc attggtgcag
     4501 cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg
     4561 actcttcgcc ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca
     4621 gttttttaat cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt
     4681 gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata
     4741 aattccagca ggccgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac
     4801 ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc
     4861 gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta
     4921 taacgatttc gtagcaattg gcagctaaat actggtctta tcagggaata taatcaacag
     4981 ctaacatgta aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa
     5041 ttagcgccat catcgacgga acttcttata acgtaaggtg aaaagctgaa aagccagccc
     5101 tgcgaagatg aggggctgca atgagataat cttcacagac cacaaataat gtagggatgc
     5161 caggatcgcg acaagataga cgacattacg cagcggttgc tggggattac ccaattttca
     5221 ctgcatcgtc tggatagatg tgaatgctaa agcacgcaaa atcaaccaac tgacaattct
     5281 cagcattaaa taggttcgag tggttaattt gtagtcaaat aaaatcagat tattcacccc
     5341 atggtaccag aaatcagtaa caggttaagc gtagtgtcgc ccaggcaaag caccataaac
     5401 ctaacaggcg acggaggcgt atcaataatg gctgtttaga ggagtatcaa tgcgcatcaa
     5461 tttgtggtca gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcctgacc
     5521 aaggtgttcg acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga
     5581 tgtcaaaatg agttccctct cttattattc cttgctaaat ggttagttaa ccttcaccag
     5641 cgtgcgaccc tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag
     5701 agatatctct tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg
     5761 ccaggcttgt gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac
     5821 attacgcaga ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca
     5881 ggccgccacg cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc
     5941 aacagtgtca attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc
     6001 atcacgaggg agaataccgc tggcacctaa acttttcaga tattcatggg tactttcgcg
     6061 accggaaacg gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc
     6121 gacgccacca ctggcacccg tcacgacaat ctcaccgtcc tgcgggcgaa caccggcatc
     6181 ttccagcgcc atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt
     6241 acgcgcgtcc agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgctc
     6301 cgccagccca ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc
     6361 ggcatgaaaa cgcggatctt cgctggtgcg tacagttccg gcaaaatcga tcccaggaat
     6421 catcggaaaa ttacggatga tttttccctt accggtaatc gccagcgcat ctttatagtt
     6481 caggctcgac cagtgaacat cgaccgtgac atcgccctcc ggcaggcgac gttcgtccag
     6541 agtctgtact gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag
     6601 tggtcctcat gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga
     6661 aaagcaaagc cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg
     6721 cttcctcact atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt
     6781 tacgctgctc accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta
     6841 caacgccatt cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac
     6901 ccaccttgtg tcgaatgact tcagcgcatt aaggccacaa attaccgaat taatggtgtc
     6961 agcagatatc gttcgtgtag acctgctgca tggtgataaa caggtttata ccctggccag
     7021 aaatggtagt tatcgtccgg ttggcaccag cgatctgttt cgtgaactga gcgttccgtt
     7081 gataaagcat ccggggatgt cgttgcgtct ggtttatcag gatccgatgg gcaactattt
     7141 ccattcgttg atgaccaccg cgccgctcac gggggcgatt ggctttatca ttcttatgct
     7201 ctttctggcg gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg
     7261 ggctactcgt atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg
     7321 gccgcccaga accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga
     7381 acaacacagc cgtcttgata cgctgatccg ctcttatgcc gcacaggacg tgaaaaccgg
     7441 cctcaataac cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa
     7501 agtaggtacc cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga
     7561 tacctggggg cacagccagg ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac
     7621 atttatgatg cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc
     7681 gctgttaccg caccggacgt taaaagaggc agagagcata gccagtcagt taatcaaagc
     7741 cgtcgatacc ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat
     7801 ctgtgcctgg cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac
     7861 gcgtaatgcg ggattgcagg gtggcaatag ctgggctatt tatgatgact cgttgcctga
     7921 aaaaggacgc ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg
     7981 cccgcgcctt tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact
     8041 catgtgccgc atcttcgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt
     8101 cttgcagttt ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact
     8161 attgcgttac tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg
     8221 tccgcgtttt cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa
     8281 acgcataatt attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc
     8341 tgttattcgt ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac
     8401 gctggtaagc accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg
     8461 gctggtcagg aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga
     8521 agcctgctcc gggaccagca cccacgttta cgccaccggc gtgcgttcgc gaagcgagtg
     8581 gcagaccctg attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca
     8641 gccacttgat actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta
     8701 atccgttttc acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc
     8761 cagattgttg cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa
     8821 tgctgctgat gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa
     8881 ctgcactaat tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg
     8941 gtaaagtaag cggattttct tttccgcccc agctttcagg attatccctt agtatgttga
     9001 aaaaatttcg tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc
     9061 tcatttatgt aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc
     9121 aggatcgtgc cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc
     9181 tgggccgtac gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg
     9241 acttcttcgt gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct
     9301 ttatgcgtcc aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac
     9361 gccgcgcaat tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag
     9421 aaccgatggc tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg
     9481 tggttgatat cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt
     9541 actcctcttc cgtgcgcatt ggtggtgatc gtttcgacga agctatcatc aactatgtgc
     9601 gtcgtaatta cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg
     9661 gttcggctta cccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag
     9721 aaggtgttcc acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac
     9781 cgctgaccgg tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg
     9841 cttccgacat ctccgagcgc ggcatggtgc tcaccggtgg tggcgcactg ctgcgtaacc
     9901 ttgaccgttt gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga
     9961 cctgtgtggc gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc
    10021 tgttcagcga agagtaatcg gatgcaggca ggggaagcgt ctgtttaccc tgcctggtct
    10081 gatacgagaa tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat
    10141 tcgccttatt ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg
    10201 gacgttcagt caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc
    10261 caatgctcct cgtgaattgc tggatggcat atcgcagacg ctggcctcgc gtgaccaact
    10321 agaacttgaa aaccgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat
    10381 gcttggacaa tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg
    10441 tcaggatgag cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga
    10501 tcaagttgtt atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag
    10561 cgacaaaggt gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct
    10621 gatttgtgat gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat
    10681 tgcagccggt aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga
    10741 tattcgtgtt ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta
    10801 tccggtcgcg gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgatcca
    10861 ggcgcgtccg actgcaggac tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga
    10921 tcgtaacggc gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct
    10981 gatgcagatg atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga
    11041 accggcaacg gggatcgctc agccgactcc gcagcaaccg gcgacaggaa atgcagctac
    11101 tgcgcctgtt gcgccgacac agcccgctgc taatcgctct ccacaaaggg ctacgccgcc
    11161 gcaaagtggt gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt
    11221 agccagggac gctgggtaat ctggctctct ttcctcattg cgctgttgct gcaaatcatg
    11281 ccctggccgg ataacctgat tgttttccgg ccaaactggg tgttacttat cttgttgtat
    11341 tggatcctgg ccttgcccca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata
    11401 ctggatctga tcagcggctc gatgcttggc gtacgcgtat tggcgatgag catcattgct
    11461 tacctggtgg cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg
    11521 gtcgtcatgt tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg
    11581 attaacgtct ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg
    11641 ccgtggattt tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt
    11701 ctatgacttc tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac
    11761 ttggcgtgac ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga
    11821 gcgcgcagca gtatgttgtg cgtctggcac gcgagaaagc acaggcgggt gtcgcgcaaa
    11881 cggcacagga tctcccggtg ctgggtgcgg atattatcgt tatcctgaac ggagaagtgc
    11941 tcgagaaacc gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga
    12001 cccatcaggt gatgacggca gtggcgttgg ccgacagcca gcacattctc gattgcctgg
    12061 tggtcaccga tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca
    12121 gcggtgaacc gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg
    12181 tcaggaagat aaatggcagc tatcacgccg tagtcggctt accgctggtt gaaacgtatg
    12241 aattattaag taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg
    12301 ttagtaaacg taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag
    12361 gaaattcata ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt
    12421 gtaagtcgtg tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc
    12481 gcgtttcttc atgcatccga catcatgccg cataccgaat gtgtggcggg tgaagaacaa
    12541 aagcaattca cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg
    12601 caggtggtga aagatccgct tggcactaaa ggtgcgcgcc tgaccaccga tatcacgctg
    12661 ccttctcgct atctggtatt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt
    12721 gaaagcgaat cagaacgtga acgtctgaaa aaagtggtcg cagagtattg cgacgagcag
    12781 ggcgggttta tcatccgcac cgcagcggaa ggggttggcg aggctgaact ggcctccgat
    12841 gccgcttatc tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg cccgcagacc
    12901 cgttatcagc tgtacggcga actggcgctg gcgcagcgtg ttctgcgtga ttttgccgac
    12961 gccgaactgg accgcattcg cgttgactca cgcctgactt acgaagcatt gctggagttt
    13021 acctcggagt acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg
    13081 attttcgatc tctttgatgt cgaaaacgaa atccagcgag cgttggaacg caaagtagaa
    13141 ctgaaatccg gtggttatct gattatcgac cagaccgaag cgatgaccac cgtggacatc
    13201 aataccggag cgtttgtcgg tcatcgcaat ctggacgaca ctattttcaa taccaatatt
    13261 gaagcgacgc aggctatcgc tcgccagtta cggttgcgta atctgggcgg gattatcatt
    13321 attgatttca tcgatatgaa taatgaagat caccgccgcc gcgtgctgca ttcgctggag
    13381 caggcgttga gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg
    13441 gtggagatga cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg taacgaatgc
    13501 ccaacctgcc acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg
    13561 cgcgagattg ttcgtgtcca ccatgcttac gactccgacc gtttcctggt ctatgcttct
    13621 ccggcagtag ccgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt
    13681 ttcgttggca aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac
    13741 gtcgtaatga tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt
    13801 tttagcagac aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc
    13861 cgcgctcgtt gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct
    13921 taacgcctgg cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga
    13981 agccagtcag ctctcagcca gctggcagaa ttttggcccg acgcttgaag cacacgacat
    14041 ccgtgcagaa ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga
    14101 tgtctggcag agcctgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct
    14161 gcgctttcgc accaacactc ctatcaccag cggtggtagc gatgacagtc tggaagccag
    14221 tcacatcagc gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt
    14281 cagttttctg acgccatccg gtcagcgcgc cgagctggcg atcccacaac tcacctggct
    14341 gaacgatcca cgtcgacacc gtgcggaagg cctggtaagc ctctccagcc ttaccggaca
    14401 gcacggcgtg atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg
    14461 tcgcgtctgg ctacaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca
    14521 ggacaatatt gctctggaaa cggcacagtt ctcccttgaa ggctggatga cgatcgacaa
    14581 aggcgatgta accggcggtg acgtctggct gaaacagggc ggtgccagct ggttgggcga
    14641 gaagcaaacg catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc
    14701 gggctggcag ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag
    14761 cggagcattg acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg
    14821 cagtgacgaa ctccggattc gcgctagtaa tctggagctg gcaggcctgg agggcatacg
    14881 cccgctggcc gcgaaacttt cacctgcact gggtgatgtt tggcgctcca cacaaccgag
    14941 cggcaagatt aacactctgg cgctggatat cccgcttcag gcggcagaca agacccgttt
    15001 tcaggcatcg tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca
    15061 cttctccggg acgctttccg gcagcgttga aaatggtttg cttaccgcgt cgatgaagca
    15121 ggcaaagatg ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc
    15181 aactataagc tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt
    15241 taaagccaaa gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga
    15301 accctggctg ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta
    15361 tttcccggaa aacttgatgg gtaaagacct ggttgattat ttaagtggtg cgattcaggg
    15421 cggtgaagcg gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa
    15481 acacaacgaa ggtcagtttg aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca
    15541 gccggactgg cctgcattaa ctaaccttga tattgaactg gactttatta acgacggttt
    15601 atggatgaaa accgatggcg ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt
    15661 gatccctgac tactcaaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa
    15721 agccgttggc ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca
    15781 agaactccag ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg
    15841 cgaactggta accgcgaaag gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc
    15901 actcgacagc atcctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca
    15961 aagtgaacca ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa
    16021 agaaggggca aaagcctacc aggttgcggt gaatctcaac ggtaactggc aaccggcgaa
    16081 aaccggcgtt ctgcctgaag cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg
    16141 taaagtgggc attgatctgc cttatcatgc tggcgcgacc tataacgtag agctgaacgg
    16201 cgatctgaat aatgtgagta gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc
    16261 actagcggta aacgttaagg ttgatggcaa tctcaacagc tttgaattaa ccggacaggc
    16321 tggtgcggat aaccatttca atagccgctg gttgcttggt caaaagctga cgctcgatcg
    16381 tgctatttgg gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga
    16441 acttaatatg ccgccgatga atggtgccga gtggctggcc ctgttccaga aaggcgctgc
    16501 ggagagtgtc ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt
    16561 gtcactggga aatcagcaat ggaataacct gagtattgtt tcgcaaccga cggcaaatgg
    16621 caccctggtt gaggcgcaag ggcgtgaaat caacgccacg ctagcgatgc gtaataacgc
    16681 gccgtggctg gcgaatatca aatatcttta ttacaacccg agcgtggcga aaactcgtgg
    16741 tgattcaacg ccgtcatcac ctttcccgac aacggagcgc attaacttcc gtggctggcc
    16801 ggacgcacaa atacgatgca cagagtgctg gttctggggg caaaaattcg gtcgcattga
    16861 cagtgatatc accatttctg gcgatacgtt aacgctgacc aatggactga ttgatactgg
    16921 tttctcgcgg cttactgccg atggtgaatg ggttaataat ccggggaatg aacgtacctc
    16981 gctgaaagga aaactgcgtg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac
    17041 gacgcccata cgccagtcgt catttaatgt ggattacgat ttacactggc gcaaagcacc
    17101 ctggcagcca gatgaagcga cgttgaatgg catcattcat actcaactgg gtaaaggcga
    17161 aattaccgaa atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc
    17221 cctgatgcgt aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga
    17281 ctccattcgc agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt
    17341 ggatggcctg gaggcggata tcgccatgaa agggtcggta aatctggtac gccgcgacct
    17401 gaatatggaa gcggttgtcg caccagagat ttctgcgacg gtgggcgtgg ctgcggcttt
    17461 tgcggttaac cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct
    17521 gtggagcaaa gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat
    17581 caacgaagtg ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta
    17641 attgccccaa tctcatatga taatcgttgc caaaggccaa cgagccggaa cataactgta
    17701 ggccggatag ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac
    17761 taacgagtag caaaaacgat gagtctgaac ctggtaagtg aacaattgct agcggcgaac
    17821 ggcctgaaac atcaggactt gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat
    17881 tatggcgatc tctattttca gtcgagctat cacgaatcct gggttttaga agaccgcatt
    17941 attaaagatg gttcttacaa catcgatcag ggcgttggcg tgcgtgcaat cagcggtgaa
    18001 aaaaccggat ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa
    18061 gcggcgcgca ccatcgtccg tgatagtggt gatggcaaag tacagacgct gggcgcggta
    18121 gagcatagcc cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag
    18181 ctggatatcc tgcgtcgcgt cgataaggtt gctcgcgaag cggacaagcg cgtacaggaa
    18241 gtgactgcca gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg
    18301 ctagcggcgg atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt cgaagaagat
    18361 ggcaaacgcg aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt
    18421 gccgatcttg acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg
    18481 ctggtcaatc tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca
    18541 ggttggccgg gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac
    18601 cgccgtggca cttcagtatt tagtggacag gtcggggagc tggtggcttc agaactgtgt
    18661 accgtggttg atgacggcac gatggtcgat cgccgtggtt cggtggcgat tgatgatgaa
    18721 ggtacgccag gccagtacaa cgtgctgatt gagaacggca ttctgaaagg ctacatgcag
    18781 gataaactca acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc
    18841 tacgcccatc tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc
    18901 ccgcaagaaa ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt
    18961 caggtggata tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa
    19021 aacggtaaag taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc
    19081 atgcagcaga tttcgatggt tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc
    19141 ggtaaagaag ggcaaagttt gccggttggc gtgggccagc caacgttgaa agtcgataac
    19201 ctgactgttg gcggtactgc gtaataattt cattattttc agaaggataa ttaatctttc
    19261 tacgtgcacg aacggtcccc tcgctcctct ggggttaggg tgaggggaac ccgttggcac
    19321 aggtttgtag aacgtaacag tacaatatga attacttctc tttcccgcgc ccgtgcatct
    19381 cctgaaacaa tttaccgacc tcaacaaaat aatccgtcag cgagttgatc acgacctgta
    19441 ccttcagcgg cagcttatct ttttcggtat ataacgcata aaccgggcgt ggatctgact
    19501 gataacgcgg cagcaggatc tccagctccc cacgattgat ctcgttgatc acccacatca
    19561 gcggcacgta ggcgatcccg gcacccgccg ttagccagcg caccagcgtc atcggatcat
    19621 tagtcacaaa tctcccttgc gggatcaggc gagtcgagat cccttccggt gcaatcagtt
    19681 caaattcatt gtcgggccgc acgctgtatt caagccatga atggctactt aaatcggcgg
    19741 gtttttccgg tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc accattggca
    19801 tcgcgccaag acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg cggatcacca
    19861 catccagacc gtcggcaatc aggtcggggg ctggaattcc ggtaaccaga ttggcgctca
    19921 aacctgggta ttctttcagc attttggctg tcagcccggc gagaacattt tgtgccatag
    19981 ttgaagaaca gccaatgcgt agcgtcccga tgggggtgtt attgaaggca tacagttgct
    20041 catgaacatc ctgcacttca tgaagcatac gacggcagcc ctggtagtaa attctaccgg
    20101 cttcggtcag gccaatgctg cgtgtgctac ggtttaacag ctttacctgc aactcatctt
    20161 ccagttttga caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg
    20221 cggtaaaaga gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca
    20281 ttattcactc tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt
    20341 acgctaatat attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc
    20401 ttctctgcgg cgacagatgc tgaaaataat aacgcctgct ctctctatac aaccaaggtc
    20461 aacatgagtc tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt
    20521 gaattgcttt tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt
    20581 atctacgact ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt
    20641 tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc
    20701 ccgtacggcc atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg
    20761 ggtctattac accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc
    20821 gatcgcgccg gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt
    20881 gaaaaaagga cagatactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga
    20941 agcgcaagcc gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg
    21001 acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggctaacaa
    21061 cgtactacaa acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa
    21121 actggatctt gaacgcacgg tgatccgagc gccagcagat ggctgggtga ccaacctcaa
    21181 cgtctatacc ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa
    21241 ctccttctat gtactggcct atatggaaga aactaagctg gaaggggtgc gtccggggta
    21301 tcgtgcagag atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt
    21361 tgccgctggg gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgaccatcga
    21421 ctctgacctt gaatgggtgc gtttggcgca acgtgttccg gttcgtattc gtctcgacaa
    21481 ccagcaagag aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca
    21541 agatcgcgac gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt
    21601 tggttaatca cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa
    21661 ctggcgaccg ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca
    21721 cgctgggcgg tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt
    21781 gaaccgtatt ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt
    21841 ggctgtattg ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt
    21901 ctggtgtgct gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa
    21961 aactcgtacg cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag
    22021 ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt
    22081 attgtgtgtg cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagta
    22141 gatcgagagc tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag
    22201 catggcgatg gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg caccacggca
    22261 ctacaaggca tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga
    22321 cgtttaaaag cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat
    22381 cttattcaga atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg
    22441 ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc
    22501 tggactgggg aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt
    22561 tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag
    22621 atcctgcaag gcgaaccgga agtaaaagta gagtcagccg aacgtcatca tgcaatggtt
    22681 aacttctggc gaaccacact ttcctgcatt ctgggcacgc atttctggct gtggacgggc
    22741 tggacttccg gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt
    22801 ttgccgaatc cacgcatggt ggcgatcgac tttatctatg ggacgctggc cgcgctgccg
    22861 ttagggctgc tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg
    22921 tgtattagcc tggcagtgct gggattcttc ctcggtatag aagtacagaa acggcgactg
    22981 ggctcgatgg gggcactggc cagcaccata aatattatcg tgctggataa cccgatgact
    23041 ttccatttca gtcagtttct cgacagcgca ttaggacaaa tcgtcggctg tgtgctcgcg
    23101 ttcaccgtta ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt
    23161 aatcagtttg tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag
    23221 aaccacctcc cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat
    23281 ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg
    23341 ccgatcccgg ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac
    23401 catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa
    23461 gaactggaaa tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa
    23521 ccggttcatc ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa
    23581 gtcaaaaccg acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag
    23641 aactcgccgc gaaacgtgac ggtggcaaca gatgaatatt tatgcctttg attttgatga
    23701 gattgagagt caggaggatt tttatcgtga ctttagccaa acctttggtc tggctaaaga
    23761 taaggtacgc gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc
    23821 acttgagatt gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat
    23881 attgctgttt gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca
    23941 ttagcgcaaa aaaaagccca cgaaccgggg gcaatatcgt cggacaaaac gatgagggtt
    24001 tatttgtaca gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg
    24061 taggccgttg cgcctttctc ttccgctttt ttgttcagca tttcacgtat atccattggc
    24121 gaagacgcca caccacttac ggatacggtc ccgattgctt cacgattttg tgcttgtgca
    24181 gcatcaatgg agtcggcagc gaatgcaccg aaagagagaa cagaaagtac gcttaatgca
    24241 gctacagtgg ttttgatttt catgattttt acctcgacat aatcttttag ctgggccttt
    24301 gtttcgtgac ccatatc
//
LOCUS       DAZPFP010000042        24184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPFP010000042 DAZPFP010000000
VERSION     DAZPFP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.042"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(25..597)
                     /locus_tag="ACHUR4_001677"
     CDS             complement(25..597)
                     /locus_tag="ACHUR4_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1455304.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACHUR4_001678"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACHUR4_001678"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1455305.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHUR4_001679"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHUR4_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1455306.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHUR4_001680"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHUR4_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1455307.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACHUR4_001681"
     CDS             complement(3559..5340)
                     /locus_tag="ACHUR4_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1455308.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACHUR4_001682"
     CDS             complement(5333..7105)
                     /locus_tag="ACHUR4_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1455309.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHUR4_001683"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHUR4_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1455310.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHUR4_001684"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHUR4_001684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1455311.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACHUR4_001685"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACHUR4_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACHUR4_001686"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACHUR4_001686"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1455312.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHUR4_001687"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHUR4_001687"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1455313.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACHUR4_001688"
     CDS             complement(11661..12032)
                     /locus_tag="ACHUR4_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1455314.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHUR4_001689"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHUR4_001689"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1455315.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHUR4_001690"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHUR4_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1455316.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHUR4_001691"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHUR4_001691"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1455317.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHUR4_001692"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHUR4_001692"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1455318.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHUR4_001693"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHUR4_001693"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1455319.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHUR4_001694"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHUR4_001694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1455320.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHUR4_001695"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHUR4_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1455321.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACHUR4_001696"
     CDS             21803..22381
                     /locus_tag="ACHUR4_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1455322.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACHUR4_001697"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACHUR4_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1455323.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5827 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccacatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DAZPFP010000043        22436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPFP010000043 DAZPFP010000000
VERSION     DAZPFP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.043"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHUR4_001698"
     CDS             82..2682
                     /locus_tag="ACHUR4_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR1455324.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHUR4_001699"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHUR4_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHUR4_001700"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHUR4_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHUR4_001701"
     CDS             4302..4817
                     /locus_tag="ACHUR4_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1455325.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHUR4_001702"
     CDS             4783..5520
                     /locus_tag="ACHUR4_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1455326.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUR4_001703"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUR4_001703"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1455327.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUR4_001704"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUR4_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1455328.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUR4_001705"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUR4_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1455329.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUR4_001706"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUR4_001706"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1455330.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHUR4_001707"
     CDS             10827..12734
                     /locus_tag="ACHUR4_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1455331.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUR4_001708"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUR4_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1455332.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUR4_001709"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUR4_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1455333.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUR4_001710"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUR4_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1455334.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUR4_001711"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUR4_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1455335.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUR4_001712"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUR4_001712"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1455336.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHUR4_001713"
     CDS             complement(17402..19162)
                     /locus_tag="ACHUR4_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1455337.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHUR4_001714"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHUR4_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1455338.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUR4_001715"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUR4_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1455339.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUR4_001716"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUR4_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1455340.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUR4_001717"
                     /pseudo
     CDS             22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUR4_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5725 a         5267 c         5879 g         5565 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tgggtagact ggccccctga atctcc
//
LOCUS       DAZPFP010000044        22192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPFP010000044 DAZPFP010000000
VERSION     DAZPFP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.044"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(467..1558)
                     /gene="ychF"
                     /locus_tag="ACHUR4_001718"
     CDS             complement(467..1558)
                     /gene="ychF"
                     /locus_tag="ACHUR4_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1455341.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1675..2259)
                     /gene="pth"
                     /locus_tag="ACHUR4_001719"
     CDS             complement(1675..2259)
                     /gene="pth"
                     /locus_tag="ACHUR4_001719"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1455342.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2537..2815
                     /gene="ychH"
                     /locus_tag="ACHUR4_001720"
     CDS             2537..2815
                     /gene="ychH"
                     /locus_tag="ACHUR4_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1455343.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2870..4549)
                     /gene="dauA"
                     /locus_tag="ACHUR4_001721"
     CDS             complement(2870..4549)
                     /gene="dauA"
                     /locus_tag="ACHUR4_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1455344.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4674..5621)
                     /gene="prs"
                     /locus_tag="ACHUR4_001722"
     CDS             complement(4674..5621)
                     /gene="prs"
                     /locus_tag="ACHUR4_001722"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1455345.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5772..6623)
                     /gene="ispE"
                     /locus_tag="ACHUR4_001723"
     CDS             complement(5772..6623)
                     /gene="ispE"
                     /locus_tag="ACHUR4_001723"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1455346.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6623..7246)
                     /gene="lolB"
                     /locus_tag="ACHUR4_001724"
     CDS             complement(6623..7246)
                     /gene="lolB"
                     /locus_tag="ACHUR4_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1455347.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7460..8716
                     /gene="hemA"
                     /locus_tag="ACHUR4_001725"
     CDS             7460..8716
                     /gene="hemA"
                     /locus_tag="ACHUR4_001725"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1455348.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8758..9840
                     /gene="prfA"
                     /locus_tag="ACHUR4_001726"
     CDS             8758..9840
                     /gene="prfA"
                     /locus_tag="ACHUR4_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1455349.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9840..10673
                     /gene="prmC"
                     /locus_tag="ACHUR4_001727"
     CDS             9840..10673
                     /gene="prmC"
                     /locus_tag="ACHUR4_001727"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1455350.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10670..11062
                     /gene="sirB2"
                     /locus_tag="ACHUR4_001728"
     CDS             10670..11062
                     /gene="sirB2"
                     /locus_tag="ACHUR4_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1455351.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11066..11875
                     /gene="sirB1"
                     /locus_tag="ACHUR4_001729"
     CDS             11066..11875
                     /gene="sirB1"
                     /locus_tag="ACHUR4_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1455352.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11911..12765
                     /gene="kdsA"
                     /locus_tag="ACHUR4_001730"
     CDS             11911..12765
                     /gene="kdsA"
                     /locus_tag="ACHUR4_001730"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1455353.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12914..13021)
                     /locus_tag="ACHUR4_001731"
     CDS             complement(12914..13021)
                     /locus_tag="ACHUR4_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1455354.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13426..14526)
                     /gene="chaA"
                     /locus_tag="ACHUR4_001732"
     CDS             complement(13426..14526)
                     /gene="chaA"
                     /locus_tag="ACHUR4_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1455355.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14796..15026
                     /gene="chaB"
                     /locus_tag="ACHUR4_001733"
     CDS             14796..15026
                     /gene="chaB"
                     /locus_tag="ACHUR4_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1455356.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15184..15879
                     /gene="chaC"
                     /locus_tag="ACHUR4_001734"
     CDS             15184..15879
                     /gene="chaC"
                     /locus_tag="ACHUR4_001734"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1455357.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15923..16276)
                     /gene="ychN"
                     /locus_tag="ACHUR4_001735"
     CDS             complement(15923..16276)
                     /gene="ychN"
                     /locus_tag="ACHUR4_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1455358.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16461..17855
                     /gene="ychO"
                     /locus_tag="ACHUR4_001736"
                     /gene_synonym="iatA"
     CDS             16461..17855
                     /gene="ychO"
                     /locus_tag="ACHUR4_001736"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1455359.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17856..18506)
                     /gene="narL"
                     /locus_tag="ACHUR4_001737"
     CDS             complement(17856..18506)
                     /gene="narL"
                     /locus_tag="ACHUR4_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1455360.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18499..20295)
                     /gene="narX"
                     /locus_tag="ACHUR4_001738"
     CDS             complement(18499..20295)
                     /gene="narX"
                     /locus_tag="ACHUR4_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1455361.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20634..22025
                     /gene="narK"
                     /locus_tag="ACHUR4_001739"
     CDS             20634..22025
                     /gene="narK"
                     /locus_tag="ACHUR4_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1455362.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5491 a         5508 c         5722 g         5471 t
ORIGIN      
        1 ttttgcatta ctaataagaa aaagcaaaaa acatcgatat tcatttttgt tcaatggatt
       61 ttctggccta tcgttattat tgtgctactg gttatcattt tcatcttgcc aggatcgcac
      121 gtaatcacgt tattcgggga ctttatctgc gggatattac ccttcaggta agtccttttc
      181 tcgccagcat attggagcga gatcaatatt gatcagctgt gaagagggct gatgtgtagt
      241 ggaacaaaaa ctgacgttat atcactgcta atgagatatc agtcatctga acacttatgt
      301 ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg atgttacaag
      361 aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt atatactttt
      421 gcgctcttca tgagatttag cgaaacctca tgagacaata aattaattag acgttgaaaa
      481 ggaagttcat cacatcgcca tctttcacga tgtaatcttt accttctgca cgcattttgc
      541 ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac gagatggttt
      601 gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc tgcggcgcgg
      661 ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg aagtaagttt
      721 gcaggttcag cagcttataa ccggcacgga tcacacggtt caggcccggt tcttccagcc
      781 caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg tctgcttcaa
      841 cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca cgcacctggt
      901 caagatatgg gttgttttca aaaccatctt cgttgacgtt ggctatgtac attgttggtt
      961 tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt aaatccagcg
     1021 cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttccaggacc gccagctcag
     1081 ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc gcacgttcgc
     1141 aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg tcagccgggt
     1201 taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca acgtgaccga
     1261 tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg cctttcgatg
     1321 cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc gtacgttgcg
     1381 gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg acgcctgtgt
     1441 tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg gtcagcgcgt
     1501 tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg aatcccatga
     1561 tttaaatcac cttaatatct taataatcaa cctgttattg ctaacagatt gcagaaatgg
     1621 aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa cgacttattg
     1681 cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa accacatttc
     1741 agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac taacaggcgg
     1801 tttgcctaac acaaaaccga caactttatt tttatcgccc ggatgaccga ttccgatgcg
     1861 taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca gtccattgtg
     1921 accaccatgg ccaccgccca acttaaattt ggcgacgcca ggaggcagat ccagttcgtc
     1981 gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg ccgcaacggc
     2041 tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt cgcttccaag
     2101 ggtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag cgcgcaaacg
     2161 ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg cgtattcagc
     2221 accggggttc gccaggccga caatcaattt aatcgtcacg tttttttgtc ctgagtgtgt
     2281 acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact gcgtatcaaa
     2341 tgcagataac gtaataattg cctgagtgga ctattagaaa gtcaaggtgt tcaggcgttt
     2401 atttgtaaag ttttgttgaa ataagggttg taattgtgat tacgcccgca cataacccac
     2461 agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac aacccagaaa
     2521 gttccggagg tgacatatga aacgcaaaaa cgcttcgata ctcggtaacg tgctcatggg
     2581 gttgggtctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc agttaccaca
     2641 gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg tcggtgccat
     2701 tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc gttactggtg
     2761 ggttcgtcac tatgacaaac gttgccgccg tagcgataat cgccgtcata gctaacaaat
     2821 aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact tataaatccg
     2881 ccatcgccgc gcgacgattc gggaagaacg cgaggcgtcc cgggatcggt tgaatgcctg
     2941 cgcgagccat agttcgcagt ggctggaatt ccacgttgca cacgcgcagt tcacatcctt
     3001 cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca agtaccggaa
     3061 cggcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc aggtccgtga
     3121 ataagccttc agcagcagca aaaaacagcg ggccaataac gcgcagaacc aggacatcgt
     3181 ctggaacatc tacgactacc ggtgccaggc gagtcatacg tgcgatacga cgcataaaca
     3241 gcagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac agcacggtca
     3301 gtgacataca cagcagcatg acgatgatgt catctttcgg cgcatgacgc agcaagtcga
     3361 ccactttgtg cgcttcactc atgttccacg ccaccatcaa cagcagggct gccatggcgg
     3421 aaagcggcag ccaggagagc agtggtgcca gtaccagcag ggcgagaata accagaatag
     3481 agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg gcagaacgcg
     3541 cgatggcagc agtagcggta ataccaccaa agaatggagc gatgatattc cccagcccct
     3601 gaccgaccag ttcactattc gccttgtgct tagtcccggt cataccatcc agtaccacag
     3661 cgcagagcag agattcgatc gccccgagca ttgccattga gaatgccgca ggcagcaggg
     3721 tgcgaataga atcccaggtt agcgtgaatt ctgaattagg cagatcccac ggcagcacca
     3781 gttgcggcag cagttgcgga ataccgttac cctgagaacc atcggccagg acgtagtgga
     3841 attgcgaacc gatggtagca acatgtccgc cgagcaggtt aacaatcccc atcaccgcgc
     3901 aaccagccag caaggccgga aggtgaccgg gtaaacgaat gcccagacgc ggccagaaaa
     3961 ccagaatgcc aagcgtcaca atgccaatgg cagcatcacc cacattaatg gtcggcagcg
     4021 ccataaataa tgcgccgact ttttgtagat aatgttccgg gacatgggcc atttgcagac
     4081 cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag gtgaaaccta
     4141 aggtgacgga aaccggaata tactcaatca gacgaccaaa gcgtgccagc cccatcagaa
     4201 tcaaaaagat tcccgacaac aaggtcgcaa ccagcagtcc tgccagtcca aactgttgcg
     4261 aaacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta aagcgtgacc
     4321 cacccgttag agcaatgaca atccccgcaa cagctgcggt atataaaccg tactgggggg
     4381 ccacaccact accaatagcc aacgccatcg ccagcgggat agcaataatc ccgacggtta
     4441 tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc cagcaaacgt
     4501 cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca ataatgtttc
     4561 atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag gtcatacaaa
     4621 tggatattac agacaaaaaa acccgccgca gcgggtcttt gagccgggtt cgattagtgt
     4681 tcgaacatgg cagagatcga ttcttcgttg ctgatacgac gaatcgcttc ggccagcata
     4741 cctgacaggg tcagagtacg cacgttcggc agtgatttga tttcatcgct cagcggaatg
     4801 gtatcgcaga caacgacttc atcaattaca gagttacgca ggttgttcgc cgcgttgcca
     4861 gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag caccacgttc tttcagcgct
     4921 tcagcagctt tacacagcgt accgccagtg tcgatcatat catcgaccag tacgcagtca
     4981 cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg cggacgacgt
     5041 ttgtcgatga ttgccatatc ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca
     5101 acgccgccga tgtccggaga aaccacaatt gggttatcca gattcagctg cagcatgtct
     5161 tccagcagga tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc
     5221 tgttcagcgt gcagatccac tgtcagcaca cggtcaacac cgacgctgga gaggaagtct
     5281 gcaaccactt tcgcagtgat tggtacacga gcggaacgga cgcgacggtc ctggcgcgca
     5341 tagccaaagt aggggataac agcggtgata cgacctgcgg aagcacgacg cagggcatca
     5401 accataacga ctaattccat caggttgtcg ttagtagggg cacaagtgga ctggatgatg
     5461 aaaatatcac caccgcgtac attttcgtta atttgtacgc tgacttcgcc gtcgctaaag
     5521 cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt
     5581 accggggtgg cgttaccagc aaaaagcttc atatcaggca cgagaagaac ctcaggcatg
     5641 cgtccattgg tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat cctttccagg
     5701 cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca
     5761 gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa gattaacgcc tttcgccaca
     5821 aagccattga gccattccgg ggcttgctct agcacctggc gggcttcaga ctctgtatca
     5881 aattcagcaa agacacaggc ccctgtccca gtcaggcgtg acggggcgta ttctaacagc
     5941 caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc gcaatcattg
     6001 ctgaattcac attttagcaa cgtttctatt gacctttttg gcgtattgcg cgggagttca
     6061 ggatctttaa aaatcaccgg agtcggaata cttacaccag ggtgcgccac cagataccac
     6121 ttctctggcg gatccaccgg cgttagtatt tcaccaacgc cttcggcaaa cgcggcatgc
     6181 ccccgaacaa agacaggaac atctgcgccc agcgtcagcc ccatttccgc cagctcatcc
     6241 atgcttagcc cgcattgcca gagatgattt aatgccacca ggaccgtcgc ggcattggat
     6301 gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt caatgctgat attcgcaccg
     6361 cttcccgtcg gaagacgccc gctgtctgcc gcagttttca tcaacaaccg cgctgcgcga
     6421 acgatcaggt tatcttcatg ttccacgcct tcaacgggcg ttaacagacg aatatcccca
     6481 tcgtcacgaa gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc
     6541 agcgtgtggt aaccatccgc acgctgaccg gtaatgtata aaaacagatt aagttttgcc
     6601 ggagagggcc actgtgtccg cattatttca ctatccagtt atccattttt aacttgatgc
     6661 gttgaccacc gtcggtgagt tccatattgg ctggcatcgc aggttgcgtt ttggtgtcat
     6721 aaccaccata aacaaccttc cagtttttgc cattctggct gtaggtaatt tcgctcaggc
     6781 ggtactggtc gtccagtttg tagtcggttg catcacccgg taaacctaaa atccactggc
     6841 gcaagctgtt gagcggaatt ggcattccgg tcaatttgcc aatcatctct tcggcgtcat
     6901 cggcggtata acgctgacct ttattgtcga ctaactgcac gttacccggt tgagcattca
     6961 gctccagttc cgtgctgccc aatgggttag tgagcagcag acggtagcga tcctggccgg
     7021 tttgctgcca gaaaaagcgg gcgtacactt tttgttggtc agaaatataa gcgaacgcgc
     7081 cgcgagtctg atactgatta agattgcgca cgtcttgctg atgctgacgc cattgtggcg
     7141 aatccgggct tttgccagga cctttgggcg tggtaacgga acaggcagtg agcacaagag
     7201 cagccagcgg tagcaggcgg ataagacgaa aatcgggcag gggcatagtg atgacaagtc
     7261 cttgagatac gttgcagtta taacccttaa tgctagcgtt accgtccgct atcgtctatg
     7321 ttcaagttgt cttaattgcc agaatctaac ggctttcggc aattactcca aaagggggcg
     7381 ctctctttta ttgatcttac gcatcctgta tgatgcaagc agactaaccc tatcaacgtt
     7441 ggtattattt cccgcagaca tgaccctttt agcactcggt atcaaccata aaacggcacc
     7501 tgtatcgctg cgagaacgtg tatcgttttc gccggataag ctcgatcagg cgcttgacag
     7561 cctgcttgcg cagccgatgg tgcagggcgg cgtggtgctg tcgacgtgca accgcacgga
     7621 actttatctt agcgttgaag agcaggataa cctgcaagag gcgttaatcc gctggctttg
     7681 cgattatcac aatcttaatg aagaagatct gcgtaaaagc ctctactggc atcaggataa
     7741 cgacgcggtt agccatttaa tgcgtgttgc cagcggcctg gattcactgg ttcttgggga
     7801 gccgcagatc ctcggtcagg ttaaaaaagc gtttgtcgat tcgcaaaaag gtcatatgaa
     7861 ggccagcgaa ctggaacgca tgttccagaa atctttctct gtagcgaaac gcgttcgcac
     7921 tgaaacagat atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg
     7981 gcagatcttt gaatcgctct ctacggtcac agtgttgctg gtaggcgcgg gcgaaaccat
     8041 cgagctggta gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta tcgccaaccg
     8101 cactcgcgaa cgtgcccaaa ttctggcaga tgaagttggc gcggaagtga ttgccctgag
     8161 tgagatcgac gaacgtctgc gcgaagccga tatcatcatc agttccaccg ccagcccgtt
     8221 accgattatc gggaaaggca tggtggagcg cgcattaaaa agtcgtcgta accaaccaat
     8281 gctgttggtg gatattgccg ttccgcgcga tgttgagccg gaagttggca aactggcgaa
     8341 tgcttatctt tatagtgtgg acgatctgca aagcatcatt tcgcacaacc tggcgcagcg
     8401 taaagccgca gcggttgagg cggaaactat tgtcgctcag gaaaccagcg aatttatggc
     8461 gtggctgcga gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc aggcagagca
     8521 cgttcgcgat gagttaaccg ccaaagcgtt agcggccctt gagcagggcg gcgacgcgca
     8581 agccattatg caggatctgg catggaaact gactaaccgc ttgatccatg cgccaacgaa
     8641 atcacttcaa caggccgccc gtgacgggga taacgaacgc ctgaatattc tgcgcgacag
     8701 cctcgggctg gagtagcagt acatcatttt ctttttttac agggtgcatt tacgcctatg
     8761 aagccttcta tcgttgccaa actggaagcc ctgcatgaac gccatgaaga agttcaggcg
     8821 ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt atcacgcgaa
     8881 tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat
     8941 atcgaaaccg cacagatgat gctcgacgat cctgaaatgc gtgagatggc gcaggatgaa
     9001 ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt tctattacta
     9061 ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag tccgtgccgg aaccggcggc
     9121 gacgaagcag cgctgtttgc aggcgatctg ttccgtatgt acagccgtta cgccgaagcc
     9181 cgccgctggc gggtagaaat catgagcgcc agcgagggtg aacatggtgg ttataaagag
     9241 atcatcgcca aaattagcgg tgatggtgtg tatggtcgtc tgaaatttga atccggcggt
     9301 catcgcgtgc aacgtgttcc tgctacggaa tcgcagggtc gtattcatac ttctgcttgt
     9361 accgttgcgg taatgccaga actgcctgac gcagaactgc cggacatcaa cccagcagat
     9421 ttacgcattg atactttccg ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat
     9481 tcggcaattc gtattactca cttgccgacc gggattgttg ttgaatgtca ggacgaacgt
     9541 tcacaacata aaaacaaagc gaaggcgctt tcagtgctcg gtgctcgcat ccacgctgct
     9601 gaaatggcaa aacgccaaca ggccgaagcg tctacccgtc gtaacctgct ggggagtggc
     9661 gatcgcagcg accgtaaccg tacttacaat ttcccgcagg ggcgcgttac cgatcaccgc
     9721 atcaacctga cgctctaccg cctggatgaa gtgatggaag gtaagctgga tatgctgatt
     9781 gaaccgatta tccaggaaca tcaggccgac caactggcgg cgttgtccga gcaggaataa
     9841 tggaatatca acactggtta cgtgaagcaa taagccaact tcaggcgagc gaaagcccgc
     9901 ggcgtgatgc tgaaatcctg ctggagcatg ttaccggcag agggcgtact tttattctcg
     9961 cctttggtga aacgcagctg actgacgaac aatgtcagca acttgatgcg ctactgacac
    10021 gtcgtcgcga tggtgaaccc attgctcatt taaccggggt gcgagaattc tggtcgttgc
    10081 cgttatttgt ttcgccagcg accttaattc cgcgcccgga tacggagtgt ctggtggagc
    10141 aggcactggc gcggttgcct gaacaacctt gccgtattct cgatctcggg acgggtaccg
    10201 gggcgattgc gcttgcgctg gctagcgagc gcccggactg cgaaattatc gctgtagatc
    10261 gtatgcctga tgctgtctcc ctggcacaac gtaatgccca gcatctggcg atcaaaaata
    10321 tccacattct gcaaagcgac tggtttagcg cgctagccgg gcagcagttt gcgatgattg
    10381 tcagcaatcc gccgtatatt gacgagcagg acccacatct tcaacaaggc gatgtccgct
    10441 ttgagccgct cactgcgctg gttgcggcag acagtggaat ggcagacatc gtgcatatca
    10501 tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct gcttctggaa catggctggc
    10561 agcagggcga agcggtgcga caagcattta tcctcgcggg gtatcatgac gtcgaaacct
    10621 gccgtgacta tggtgataac gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt
    10681 ttctacactg cttagtgttc atcttattag tatcgcgctt tctgttgggc tattaacctt
    10741 acgtttctgg ctacgttatc agaagcatcc acaggcattt gctcgctgga cgcgcattgt
    10801 gccgccggtt gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca
    10861 catcctgcca ttttccgggc aggcacagtg gctgactgaa aagctgtttg gagttatcat
    10921 ttatatcgtt ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc agcaggcgcg
    10981 cattattgcc ttcccgctgg cgttggtggt gctgtacatc atcattaaac tcgccaccac
    11041 aaaagtaccg ttactggggt aagtcatgag atcgttagct gatttcgaat ttaataaagc
    11101 gccattgtgc gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca
    11161 agatgtttac gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa tcagccagct
    11221 tctgccttta gaagagcagc tggaaaaact gatcgcgctg ttttacggcg actggggatt
    11281 taaagcctca cgcggcgttt atcgtctttc cgatgcatta tggctggacc aggtgttaaa
    11341 gaatcgacag ggcagtgcgg tatcattagg tgcggtttta ttatgggtcg cgaatcgtct
    11401 cgatttgccg ctgctgccgg tgattttccc tacgcagctg atattgcgca ttgaatgtcc
    11461 ggatggcgaa atttggctga ttaatccttt taacggtgaa tcgttaagcg aacatatgct
    11521 ggacggatgg ttaaagggaa atatcagccc gtcggcggaa ctgttttatg aagaccttga
    11581 tgaagctgat aacattgagg taatccgcaa attgctggat acactcaaag cctcgttgat
    11641 ggaagaaaat cagatggagc tggcgttacg caccagcgaa gctttattac aattcaaccc
    11701 tgaagatccc tatgaaattc gcgatcgtgg gttgatttat gcgcaactgg attgcgaaca
    11761 cgttgcgttg aacgatttaa gttatttcgt tgaacggtgt ccggaagacc cgatcagcga
    11821 aatgatccgt gcgcaaataa ataacatcgc gcataaacat attgtgctgc attaattaat
    11881 cgacatttta ctcaagatta aggcgatcct atgaaacaaa aagtggttag cattggcgac
    11941 atcaacgtag caaatgacct gccgttcgta ctgtttggcg gtatgaacgt gctggaatct
    12001 cgcgatctgg cgatgcgcat ttgcgagcac tacgtaactg tgacccagaa actgggtatc
    12061 ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct cctccatcca ctcttatcgt
    12121 ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt tgaagcagac ttttggcgtg
    12181 aaaattatca ccgacgttca cgaaccaagt caggcacagc ccgttgctga tgtcgtggat
    12241 gtgattcagt tgccggcgtt tcttgctcgc cagactgacc tggttgaagc catggcgaaa
    12301 accggtgcgg taattaacgt caagaaacca cagtttgtca gcccgggaca gatgggtaat
    12361 atcgttgata aattcaaaga aggcggcaac gaaaaagtga ttctttgcga tcgtggtgct
    12421 aacttcggct atgacaacct ggttgtcgat atgctgggct tcagcattat gaagaaagtg
    12481 tctggtaact cgccggtgat tttcgacgtg acccacgcac tgcaatgccg cgatccgttt
    12541 ggcgcagctt ccggtggtcg tcgtgctcag gtggctgagc tggcacgagc cggtatggcg
    12601 gtaggtctgg cggggctgtt tattgaagcg catccggatc cggaacatgc gaaatgtgat
    12661 ggtccatccg cgctgccgct ggctaaactg gaaccgttcc tcaagcagat gaaagcgatt
    12721 gatgatctgg tgaaaggttt cgaagaactg gataccagca agtaatcttt tttgcttgaa
    12781 aaataaagtc ttagcgttct gcgttaagac ttttttcatg ggtgccagat acaaaaaagg
    12841 ccgcaggctg ttacccctgc ggccggtttc gggcgcatat tgccatcacg gcagcctgac
    12901 gcccgttttc accttacttc cggttacgcc accagctgac aatcgctgcg gcaataatcc
    12961 ccgccaggat cggtgctgcc aggtcgtgcc agaaagtcat ggcaaactgc gcgagcgtca
    13021 tatagccgcc ttgttgtaat gacaacattt tgcggctatt cttgaagtgt ctggtttcaa
    13081 gattagcccc cgttttgttg tcaggtttta cctctcaacg tgcgggggtt ttctctttcc
    13141 agcaaccaat gccaccaggg ataaagcccc cgcaacattg cgcctcaccg gataattccg
    13201 gcttggtgtg gatactacgt ctcaattcat cttcacttca tccctgaaat gtttgcaata
    13261 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
    13321 gctcgcaatt ctgattaacg agctgtccag gtagtatgaa gaaatacaat aagcaggaat
    13381 ttatcttatc tctcccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
    13441 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
    13501 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
    13561 accaagtgca aactgcaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
    13621 ggtaagcgaa attgttgcta acactgaacc aaagaacaga ttcatcgcgc gctgaacctg
    13681 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagtgccac
    13741 caggaagcca gtaaaggcaa caggggcatt catgctgtcg agcaatgtct ccagcgagct
    13801 ggcgttcatt ttggtgactg caataacggc aatcagatgg ataatcaacc agatagcatg
    13861 ccacaggctg ctatgggcag acgttttacc gtggtgcggg tcgtcgtcat cactgtcatc
    13921 ttcgtgctcg tagacaaaca aactttgatg cgttttggcc tggatcagca aaaatacgcc
    13981 atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc
    14041 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
    14101 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
    14161 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
    14221 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
    14281 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
    14341 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
    14401 gatggcaatg accactggta gtgtctggct acttccccac aggaacagca ctaccagcac
    14461 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
    14521 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa
    14581 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cgctgtcgat
    14641 tttttactca ttggggcata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
    14701 tcttaagaac agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
    14761 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
    14821 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
    14881 tcaacagcgc atgggatcaa tataaagata aagaagatcg gcgtgatgac gccagtcgcg
    14941 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
    15001 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
    15061 ttgccttgca agtggtccgt ggattgcata ttgttccgtt agtggtttca aaatgagcgg
    15121 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
    15181 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
    15241 gaagaatcac tcttatggtc agcagaacaa cgggcggctt cgctggcggc gacgctggct
    15301 tgtcgacctg atgagggacc ggtgtggatc ttcggttatg gatcgttgat gtggaatccg
    15361 gcactggagt ttaccgaatc atgcaccggt acactggttg gatggcatcg cgcgttttgc
    15421 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
    15481 gagggcggac gcaccacagg cgtcgcctat cgactaccag aagagacgct ggagcaggaa
    15541 ctaaccctgt tgtggaagcg cgagatgatt accggctgtt atctgccaac ctggtgtcag
    15601 cttgatcttg atgatggacg cacagtaaac gccattgtgt ttattatgga cccgcgacat
    15661 ccagaatatg aatctgacac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
    15721 ggtccgctgg gaaccaatgc acaatacctg ttttcactgg aacaggagct tatcaaactg
    15781 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact actggcggag
    15841 aattatccgg atggtgtgtt aaggccggga ttcgcctgag taaacttccc tcatagtggg
    15901 gcgtcagacg cccccttaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
    15961 gcgccagttc cacaagagta ccgatttcca ccccatcaat cagaggaagt gtgctaatcc
    16021 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
    16081 ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga
    16141 ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
    16201 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
    16261 tcactatttt ttgcattatt tactcctgta ttcagggaat tagacactca tcttctatct
    16321 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
    16381 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
    16441 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
    16501 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
    16561 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
    16621 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
    16681 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
    16741 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg
    16801 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
    16861 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggata
    16921 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
    16981 gttataacac tttttatgac aacttgcagg acgaaaatct tcagcgagcg ggctttggtg
    17041 ccgaagcgtg gggcgaatat ttgcgattat cggcaaactt ttatcagccg tttgctgcat
    17101 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtacgac ctgacagctc
    17161 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcctagaa cagtattttg
    17221 gtgatcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
    17281 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacaggggg
    17341 aaagtggcga aaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
    17401 tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
    17461 atgacaatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
    17521 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
    17581 tacaaatccg cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat
    17641 taagtttgac gccgggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
    17701 actggcagaa cggggaaggg gcaagcaatc actggcgatt gtctgtggtg gtggaagata
    17761 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg
    17821 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
    17881 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
    17941 acttttactg tgctttcggt gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc
    18001 tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgctca
    18061 gtagtggcac ggttagcgcg caagctggcg gccagaacag gcgttaatgc ttcgcttaat
    18121 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
    18181 tttaacagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
    18241 gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
    18301 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagat
    18361 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt
    18421 gccatactga taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg
    18481 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
    18541 gggaataaag gtgaccacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc
    18601 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
    18661 ggcattttca ggcacgccac agccgttatc ctggacggtc agtttgacct gattatcgtt
    18721 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
    18781 ctcacgggca atttgcaaca agtggattgc ctgatgtgaa ggcaccagac gaggcggcaa
    18841 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcttc
    18901 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
    18961 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
    19021 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag
    19081 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
    19141 caactgttgc tgacgttcct gatggcgatc cagcgccagc gtggcggtaa gttgttcaac
    19201 cagggtatcc accagttgtt gttggtcatg gctaagatga cgcccctgcg gcagggtcgc
    19261 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg tcgtgccgcg
    19321 atcaccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
    19381 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcag
    19441 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtaccggtg acaggcgttc
    19501 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg
    19561 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
    19621 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
    19681 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
    19741 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
    19801 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcaa tacgcatttc
    19861 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
    19921 cgctgacacc gtttctcggt tttgtgcacg catcagcgca gggatcagtt cattacgcca
    19981 gtaatcttgt aagccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
    20041 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
    20101 tggcactgcc gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
    20161 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
    20221 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
    20281 acaacgttta agcatgtaaa ccccttcctt caggctttaa atgagcaata accttaatga
    20341 atgtgacgat atattctgga atggcagtat tctcggctat tggctgaagt atacccatag
    20401 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
    20461 gattctacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
    20521 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
    20581 tttagctcca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
    20641 actcatctgc ctccgaaagg gctactggag ctgtcataac agattggcga ccggaagatc
    20701 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc
    20761 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
    20821 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg
    20881 tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc
    20941 gctggacggc gttcagcacc ggtattctga ttattccttg cgtctggctg ggttttgccg
    21001 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgcta tgcggctttg
    21061 ctggcgcgaa cttcgcatcc agtatggcaa acatcagctt cttctttccg aaacagaagc
    21121 agggtggcgc gctgggtctg aatggtggtc tgggaaacat gggcgtcagc gtcatgcagt
    21181 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
    21241 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
    21301 ttgccatctt caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
    21361 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
    21421 atctggcaac cttcggctcc ttcatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
    21481 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
    21541 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
    21601 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg
    21661 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
    21721 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
    21781 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccggca
    21841 cggcagcggc gctgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga
    21901 aagcgtttgg tagctcgctg gcattaacgg gttcgccagt aggtgcaatg aaagtatttt
    21961 tgattttcta tatcgcctgc gtagtgatta cttgggcggt atatggtcgg cattctaaaa
    22021 aataaatctt aatatttgat tatcctttgc gcggcatgat gccgcgcttt ttttatgcgt
    22081 catttagtta caacatatta gtgttatatg gcttatttcg ccggatttca ttaagagcca
    22141 ttaatatgtt acccatgggg aatactcctt aatacccatt tgcataaaaa tc
//
LOCUS       DAZPFP010000045        22001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPFP010000045 DAZPFP010000000
VERSION     DAZPFP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.045"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..165)
                     /locus_tag="ACHUR4_001740"
     CDS             complement(<1..165)
                     /locus_tag="ACHUR4_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1455363.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQ"
     gene            complement(175..726)
                     /gene="ybcL"
                     /locus_tag="ACHUR4_001741"
     CDS             complement(175..726)
                     /gene="ybcL"
                     /locus_tag="ACHUR4_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1455364.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(895..1227)
                     /gene="emrE"
                     /locus_tag="ACHUR4_001742"
     CDS             complement(895..1227)
                     /gene="emrE"
                     /locus_tag="ACHUR4_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1455365.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1572..1886)
                     /gene="fliE"
                     /locus_tag="ACHUR4_001743"
     CDS             complement(1572..1886)
                     /gene="fliE"
                     /locus_tag="ACHUR4_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1455366.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2100..3759
                     /gene="fliF"
                     /locus_tag="ACHUR4_001744"
                     /pseudo
     CDS             2100..3759
                     /gene="fliF"
                     /locus_tag="ACHUR4_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3752..4747
                     /gene="fliG"
                     /locus_tag="ACHUR4_001745"
     CDS             3752..4747
                     /gene="fliG"
                     /locus_tag="ACHUR4_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1455367.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4740..5426
                     /gene="fliH"
                     /locus_tag="ACHUR4_001746"
     CDS             4740..5426
                     /gene="fliH"
                     /locus_tag="ACHUR4_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1455368.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5426..6799
                     /gene="fliI"
                     /locus_tag="ACHUR4_001747"
     CDS             5426..6799
                     /gene="fliI"
                     /locus_tag="ACHUR4_001747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1455369.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6821..7162
                     /gene="fliJ"
                     /locus_tag="ACHUR4_001748"
                     /pseudo
     CDS             6821..7162
                     /gene="fliJ"
                     /locus_tag="ACHUR4_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7159..8286
                     /locus_tag="ACHUR4_001749"
     CDS             7159..8286
                     /locus_tag="ACHUR4_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1455370.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8391..8855
                     /gene="fliL"
                     /locus_tag="ACHUR4_001750"
     CDS             8391..8855
                     /gene="fliL"
                     /locus_tag="ACHUR4_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1455371.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8860..9864
                     /gene="fliM"
                     /locus_tag="ACHUR4_001751"
     CDS             8860..9864
                     /gene="fliM"
                     /locus_tag="ACHUR4_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1455372.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9861..10274
                     /gene="fliN"
                     /locus_tag="ACHUR4_001752"
     CDS             9861..10274
                     /gene="fliN"
                     /locus_tag="ACHUR4_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1455373.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10277..10642
                     /gene="fliO"
                     /locus_tag="ACHUR4_001753"
     CDS             10277..10642
                     /gene="fliO"
                     /locus_tag="ACHUR4_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1455374.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10642..11407
                     /gene="fliP"
                     /locus_tag="ACHUR4_001754"
                     /pseudo
     CDS             10642..11407
                     /gene="fliP"
                     /locus_tag="ACHUR4_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11417..11686
                     /gene="fliQ"
                     /locus_tag="ACHUR4_001755"
     CDS             11417..11686
                     /gene="fliQ"
                     /locus_tag="ACHUR4_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1455375.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11695..12480
                     /gene="fliR"
                     /locus_tag="ACHUR4_001756"
     CDS             11695..12480
                     /gene="fliR"
                     /locus_tag="ACHUR4_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1455376.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12765..13389
                     /gene="rcsA"
                     /locus_tag="ACHUR4_001757"
                     /pseudo
     CDS             12765..13389
                     /gene="rcsA"
                     /locus_tag="ACHUR4_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13433..13621)
                     /gene="dsrB"
                     /locus_tag="ACHUR4_001758"
     CDS             complement(13433..13621)
                     /gene="dsrB"
                     /locus_tag="ACHUR4_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1455377.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13784..14015
                     /gene="yodD"
                     /locus_tag="ACHUR4_001759"
                     /pseudo
     CDS             13784..14015
                     /gene="yodD"
                     /locus_tag="ACHUR4_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            14313..15128
                     /locus_tag="ACHUR4_001760"
                     /pseudo
     CDS             14313..15128
                     /locus_tag="ACHUR4_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15125..16819)
                     /gene="dgcQ"
                     /locus_tag="ACHUR4_001761"
                     /pseudo
     CDS             complement(15125..16819)
                     /gene="dgcQ"
                     /locus_tag="ACHUR4_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16990..17172)
                     /locus_tag="ACHUR4_001762"
     CDS             complement(16990..17172)
                     /locus_tag="ACHUR4_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1455378.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17251..18168)
                     /locus_tag="ACHUR4_001763"
     CDS             complement(17251..18168)
                     /locus_tag="ACHUR4_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1455379.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18341..19261
                     /gene="yedA"
                     /locus_tag="ACHUR4_001764"
     CDS             18341..19261
                     /gene="yedA"
                     /locus_tag="ACHUR4_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR1455380.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19250..19720)
                     /gene="vsr"
                     /locus_tag="ACHUR4_001765"
     CDS             complement(19250..19720)
                     /gene="vsr"
                     /locus_tag="ACHUR4_001765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1455381.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19701..21119)
                     /gene="dcm"
                     /locus_tag="ACHUR4_001766"
     CDS             complement(19701..21119)
                     /gene="dcm"
                     /locus_tag="ACHUR4_001766"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1455382.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21186..21687)
                     /locus_tag="ACHUR4_001767"
                     /pseudo
     CDS             complement(21186..21687)
                     /locus_tag="ACHUR4_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21766..>22001)
                     /gene="drpB"
                     /locus_tag="ACHUR4_001768"
     CDS             complement(21766..>22001)
                     /gene="drpB"
                     /locus_tag="ACHUR4_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1455383.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         5391 a         5801 c         5591 g         5218 t
ORIGIN      
        1 ctgattatta tcaacagtta gactatgcgt ctcccaattg ataacttttt ttccctgacg
       61 gatacgacat aaagctggca taagaggctt aaccctgtga atctcatgat gtttatgcat
      121 ccgtatttct tcaatcttta ggttagactt atctcttgcc agcatactct taccctattt
      181 tatctcataa actggtgtta tctcagcggt tgcaatttta ttggcattaa gcatataacc
      241 aactaacgct ccgctggaat tagaatccac aggaatctta tcagttttta gagcccatac
      301 tttaaactgg taatgatggg gtttatctcc tttgggagga catgctccac caaatcctgc
      361 ataaccaaaa tcatttcggc cttgaacagc accagtcgga agttttgtac catcacgtct
      421 tcctgcatca gtgggcaaat atgttacagt tgctggaata ttagcaacag tccagtgcca
      481 ccatccactg cctgtaggag catctggatc atacacagtt acggcaaagc ttttggtgcc
      541 ttcaggagca ccagaccagg ttaatgaggg cgatgtatta ccaccttcac acccaaatcc
      601 agaaaataca tgagacgttg taagttgctc tcctgttttt atttcattac tagtgacctg
      661 aaatgctgcg gcctgcgcag aaaatgttat gaatgccaat acacttgaaa cgataagttt
      721 tttcataaaa acctcattgt tgtgacctat cgttatttta tttgatgtgc cttgatctca
      781 ttatgcataa aggcgcaatg atcgcgcaaa tgcaatcata attataaagg tgccccgaac
      841 attagccata atatgcacaa atcagattcc tgttatctca aggagaatta ttttttaatg
      901 cggtgcactt cgtgacaaca agttaatgac taacacaccg gcacaaatca acatcatgcc
      961 tatgatagct ggcaggtcca gtcgttgacc aaaaaatccc caggaaagca aactaatcag
     1021 aacgataccg actcctgacc agatagcata agcaatccct gtaggtatat aagccagcgt
     1081 ctgagctaat aaccagaatg atgcacaata gcaaataatt gtgccaacag agggccataa
     1141 ccgtgtaaac ccctctgaaa acttcattaa ggttgtacca atgacctctg caagtattgc
     1201 gccaccaaga taaatataag ggttcatagc atattctttc ctgttcatac tggagagaat
     1261 tgtactacag tttgaacaca actcaccgtt ttcatcctgg tgtatgtgta tccaaggggc
     1321 tctggcgtgg caagagataa aggcaggaac aataatgatc agaaggaacc ccaggctaac
     1381 gaatgagttc actcttcctg ggagcaaagc gaagacaaaa catgttatcg cgtttacgga
     1441 tcgtggatgg cagaaaacaa ccaggatcag gtatccattt taaaccagaa attgagtgag
     1501 tttgccccat ccatgcccca cgctgtggga tcggatataa ttaaacaagc atagtcaaca
     1561 ggttacacaa cctacacctg catggacatc acttcctgat acgccgccac cagcttatta
     1621 cgcacctgaa tccccatttg catagaaact gaggcttttt gcatatcggt catcacatcg
     1681 tttagcgcca cgccgggttc accgagggtg aatttttctg cctgcgtgcg ggcgactgtt
     1741 tgtgtatcgc ttatgcgatc gagcgcggcg tgcagctgcc cggcaaaact aatggtcggt
     1801 tgcggcagtg attcctgcgc acgcgcactc atcgccgtag cctgtaactg gctgataacc
     1861 ccttcaatcc cctgtatcgc tgacattttc gtctcccgga taatttctgg taacaaagcc
     1921 taccagtaag tcaataagac aaaggcgcta aatagcaaca aaaaaactgg tttattggcg
     1981 gatagaaaaa acgaaagcac aaataatggg agcgtcaatt tttcgagtgt gctgacccga
     2041 gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg aagtgcgcga
     2101 tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc
     2161 cgaaaattcc attgattgtt accggttccg cggcagtggc ggtcatggtc gcactgatcc
     2221 tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg
     2281 gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg
     2341 gcgctattga ggttccggca gataaagttc acgaactgcg cctggcgcaa caaggtttgc
     2401 ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt
     2461 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa
     2521 ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
     2581 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
     2641 tcgatgaagg gcaaattagc gctattgtac atctggtttc cagcgcggtt gctggtctgc
     2701 cgcctggaaa cgtcacgctg gtggatcagg gcgggcatct gttaacccag tccaatacca
     2761 gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc
     2821 agcagcgtat tgaagcgatc ctgtcaccta ttgttggtaa cggtaatatt cacgcctagg
     2881 tcacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
     2941 gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
     3001 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg
     3061 cgccaatcag cacgcctccg acaaatcaaa ataaccgcca gcagcaggcg agcaccacca
     3121 gcaatagtgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
     3181 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg
     3241 tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc
     3301 aaattgaagc tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca
     3361 atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagcactg ccgttttggc
     3421 aacagcaagt gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gttgctggta
     3481 ctgctggtgg cgtggttgct gtggcggaaa gcggtacgcc cgcagctaac acgtcgcgcc
     3541 gaggcggtga agactgtgca gcaacaggcg caggcccgcg aggaagtgga agatgcggtg
     3601 gaagtccgtc tgagcaaaga cgaacaactc caacaacggc gcgctaacca acgactgggg
     3661 gcagaagtca tgagccagcg tatccgtgaa atgtctgata acgatccgcg cgtggtggcg
     3721 ctggtcattc gccagtggat aaataacgat catgagtaac ctgacaggca ccgataaaag
     3781 cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca gaggtgttca agcacctctc
     3841 ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga tctccaacaa
     3901 gcagctaacc gatgtgctgg cggagtttga gcaagaagct gaacagtttg ctgcactgaa
     3961 tatcaacgcc aacgattatc tgcgttcggt attggtcaaa gctctgggtg aagaacgtgc
     4021 cgccagcctg ctggaagata ttctcgaaac ccgcgatacc gccagcggta ttgaaacgct
     4081 caactttatg gagccgcaga gcgccgccga tctgattcgc gatgagcatc cgcaaattat
     4141 cgccaccatt ctggtgcatc tgaggcgcgc ccaggccgcc gatattctgg cgttgttcga
     4201 tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc tttggcggcg tgcagccagc
     4261 cgcgctggcc gaactgaccg aagtactgaa tggcttgctc gacggtcaga atctcaagcg
     4321 cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc aacctgatga aaacgcagca
     4381 ggaagaagcc gttattaccg ccgtgcgtga attcgacggc gagctggcgc agaaaatcat
     4441 cgacgagatg ttcctgttcg agaatctggt ggatgtcgat gatcgcagca ttcagcgtct
     4501 gttgcaggaa gtggattccg aatcgctgct gatcgcgctg aaaggggccg agcagccact
     4561 gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc gcgacgatct
     4621 cgccaagcgt ggtccggtgc gtctgtcgca ggtcgaaaac gaacagaaag cgattctgct
     4681 gattgtgcgc cgtcttgccg aaactggcga gatggtgatt ggcagcggcg aggataccta
     4741 tgtctgataa tctgccgtgg aaaacctgga cgccggacga tctcgcgcca ccaccggcag
     4801 agtttgtgcc aatggtcgag tcggaagaaa ccattattga agaggccgaa cccagccttg
     4861 agcagcaact ggcgcaactg caaatgcagg cccacgagca gggttatcag gcggggattg
     4921 ccgaaggtcg ccagcaaggt cacgaacagg gctatcagga aggactggcc caggggctgg
     4981 aacaaggtct ggcagaggcg aaggctcaac aagcgccaat tcatgcccgg atgcagcaac
     5041 tggtcagcga atttcaaact acccttgatg cgcttgatag tgtgatagct tcgcgcctga
     5101 tgcagatggc gctggaggca gcacgtcagg tgatcggtca gacaccaacg gtggataact
     5161 cggcactgat caaacagatc caacagttgt tgcagcaaga accgttattc agcgggaaac
     5221 cacagctgcg cgtacacccg gatgatctgc aacgtgtgga tgatatgctc ggcgcaactt
     5281 taagtttgca tggctggcgc ttgcggggcg atcccaccct ccaccctggc ggatgtaaag
     5341 tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac tcgctggcaa gaactctgcc
     5401 gtctggcagc accaggagtg gtgtaatgac cacgcgcctg actcgctggc taaccacgct
     5461 ggataacttt gaagccaaaa tggcgcagtt gcctgcggta cgtcgctacg ggcgattaac
     5521 ccgcgctacc gggctggtgc tggaagccac cggattacaa ttgccgctcg gcgcaacctg
     5581 tgtcattgag cgccagaacg gcagcgaaac gcacgaagta gaaagcgaag tcgttggctt
     5641 taacggtcaa cggctgtttt taatgccgct ggaggaagtg gaaggtgtcc tgcccggcgc
     5701 gcgtgtatat gccaaaaaca tttcggcaga agggctgcaa agcggcaagc agttgccgct
     5761 cggtccggcg ttattaggtc gtgttctgga cggtagcggt aaaccgctcg atggcctgcc
     5821 cttccccgat acgacggaaa ccggtgcgct gattaccccg ccatttaacc cgttgcaacg
     5881 tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca atcaatgccc tgcttaccgt
     5941 cggacgcggg cagcgtatgg ggctgtttgc cgggtcgggc gttggtaaaa gtgtgctgct
     6001 ggggatgatg gcacgctaca cccgcgccga tgtcattgtc gtgggtttga ttggtgaacg
     6061 tgggcgcgaa gtaaaagatt ttattgagaa tatcctcggt gccgaaggac gtgcacgctc
     6121 agtggtgatt gccgctccgg cggatgtttc tccgctcctg cgaatgcaag gtgccgccta
     6181 tgccacgcgc attgccgaag attttcgcga tcgtggtcag catgtgttgc tgattatgga
     6241 ctccctcacc cgctacgcga tggcccagcg tgaaattgcg ctggcgattg gcgaaccacc
     6301 tgccactaaa ggttatccac cgtcggtgtt tgccaaatta ccggcactgg tcgagcgtgc
     6361 cggtaatggc attagcggcg gcggctcgat taccgcgttt tataccgtgc tcaccgaagg
     6421 cgatgaccag caggacccca ttgctgactc cgcgcgggca atcctcgacg gtcacattgt
     6481 gctgtctcgc cgactggcgg aagccgggca ctatccggct atcgatattg aagcgtcgat
     6541 cagtcgcgca atgacggcgt tgatcagtga gcaacattac gcgcgagtgc gcaccttcaa
     6601 acagctgttg tcgagttttc agcgtaaccg cgatctggtt agcgtcggcg cgtatgccaa
     6661 aggcagcgat ccgatgctcg ataaagccat cgccctgtgg ccgcagctgg agggctattt
     6721 gcaacaaggc atttttgaac gcgcggactg ggaagcgtct ctccaggggc tggagcgtat
     6781 tttcccgaca gtgtcataac ccaggagata acggcagatg gcggaagaac agctcaaaat
     6841 gttgatcgat tatcagaatg aatatcgcaa taacctcaat agcgatatga gtgccgggat
     6901 gaccagcaac cgctggatca actatcagca gtttatccag acgctggaaa aagccattac
     6961 tcagcatcgc cagcaactta atcagtggac gcagaaagtt gacattgccc tgaacagctg
     7021 gcgagaaaag aaacaacgtt tgcaggcctg gcagacactg caggaacggc aatccacggc
     7081 ggcactgctt gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc
     7141 cgccatgagg aaacctgaat gattcgctta gcgcccttga ttaccgccga cgttgacacc
     7201 accacattgc ctggcggcaa agccagcgat gctgcacaag attttctcac gttgttgagc
     7261 gaagcattag caggcgagac aacaactgac aaagcggccc cccagttgct ggtagcaaca
     7321 gataagccca cgacaaaagg cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa
     7381 gcggatttac tgatccctgt ggatgaaaca cttcctgtca tcaacgacga acaatccaca
     7441 tcaacaccgt taaccaccgc tcagacgatg acgttggctg cggtggctga caaaaatacg
     7501 acaaaagacg aaaaagcgga tgatcttaat gaagacgtca ccgcaagtct gagcgccctt
     7561 tttgcgatgt tgccgggttt tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg
     7621 ttaccggcag agaaaccaac gctcttcaca aaactgactt ctgcgcaact cactacagca
     7681 cagcctgatg acgcccccgg cacaccagct cagccattaa caccgctggt agcagaagcc
     7741 cagagtaaag cggaagtcat cagcacgcct tcgccggtga ccgctgacgc cagcccgcta
     7801 atcactccac accagacaca gccactgccc accgtcgccg cgcctgtttt gagtgcaccg
     7861 ctgggttctc acgaatggca acaatcattg agccagcata tttcgctgtt cacccgccag
     7921 gggcaacaaa gtgcagagtt gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc
     7981 ctcaaagtgg atgataacca ggcgcagatc cagatgattt caccgcatca gcacgtacgc
     8041 gccgccctgg aagcagcgct gccggtactg cgtacgcagc tggccgaaag tggcattcag
     8101 ttagggcaaa gcaacattag tggcgaaagc tttagtggtc agcagcaggc cgcttcccag
     8161 caacagcaaa gccaacgcac agcaaaccat gaacctctgg cgggggaaga cgacgatacg
     8221 cttccggttc ccgtctcttt acaagggcgt gtaacaggta acagcggcgt tgatattttc
     8281 gcctaacgtc agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga
     8341 cgcaggataa ttagccgata agcagtagcg acacaggaag accgcaacac atgactgatt
     8401 acgcgataag caagaaaagc aagcgatcgc tttggatccc gattctggta ttcattaccc
     8461 tcgcggcctg tgccagcgca ggttacagct actggcattc gcatcaggtt gccgctgacg
     8521 acaaagcgca gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat acctttacgg
     8581 tcaatttggg cgatgcggat cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg
     8641 aagcaacccg ctcgcggctg agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac
     8701 tgttttcacg tcaggatgct gccgtactgg cgacagaaga aggcaagaaa aacctgattg
     8761 ccgagattaa aaccacactt tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca
     8821 ccgacgtgct gtataccgct tttattctgc gataacgaca tgggcgatag tattctttct
     8881 caagctgaaa ttgatgcgct gttgaatggt gacagcgaag tcaaagacga accgacagcc
     8941 agtattagcg gcgaaagtga cattcgtccg tacgatccga atacccaacg acgggttgtg
     9001 cgcgaacgtt tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg
     9061 gggctgttca acctgctgcg ccgtagcccg gacattaccg tcggggccat ccgcattcag
     9121 ccgtaccatg aatttgcccg caacctgccg gtgccgacca acctgaacct aatccacctg
     9181 aaaccgctac gcggcacagg gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg
     9241 gataacctgt ttggcggcga tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc
     9301 catactgaac agcgcgtcat caaccgtatg ttgaagctgg cgctggaagg ctatagcgac
     9361 gcctggaaag cgattaatcc gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa
     9421 tttaccaata tcaccacctc gccgaacgac attgtggtta acacgccgtt ccatgtggag
     9481 attggcaacc tgaccggcga atttaatatc tgcctgccat tcagcatgat cgagccgcta
     9541 cgggaattgt tggttaaccc gccgctggaa aactcgcgta atgaagatca gaactggcgc
     9601 gataacctgg tgcgccaggt gcagcattca cagctggaac tggtggccaa ctttgccgat
     9661 atctcgctac gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa
     9721 aaacccgatc gcatcatcgc ccatgttgac ggcgtcccgg ttctgaccag tcagtacggt
     9781 actctcaacg gtcagtatgc gttacggata gaacatttga ttaacccgat tttaaattct
     9841 ctgaacgagg aacagcccaa atgagtgaca tgaataatcc ggccgatgac aacaacggcg
     9901 caatggacga tctgtgggct gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg
     9961 ctgccgacgc ggtgttccag caatttggcg gtggtgatgt cagcggaacg ttgcaggata
    10021 tcgacctgat tatggatatt ccggtcaagc tgaccgtcga gctgggccgt acgcggatga
    10081 ccatcaaaga gctgttgcgt ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg
    10141 gcgaaccgct ggatattctg atcaacggtt atttaatcgc ccagggcgaa gtggtggttg
    10201 ttgccgataa atatggcgtg cggatcaccg atatcattac cccgtccgag cggatgcgcc
    10261 gcctgagccg ttagtgatga ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc
    10321 tgagccactg ctgcaggtga gcggcgcact catcgccatt attgccctga tcctcgctgc
    10381 tgcctggctg gtaaaacggc tgggatttgc ccctaaacgc actggcgtta acggtctgaa
    10441 aattagcgcc agtgcttcgc tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga
    10501 tgcacggctg gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata aacttccccc
    10561 ttcagcacca acggaagaga taccgcagac cgattttcag tcggtcatga aaaatttgct
    10621 taagcgtggc gggagatcct gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg
    10681 ctgattacgc ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc gctacctggc
    10741 gggtatcacc agccagccgc tacctggcgg tggacaaagc tggtcgctcc cggtgcagac
    10801 gctggtgttc atcacctcgt tgacgttcat tccagcgatt ttactgatga tgaccagttt
    10861 cacccgcatc attattgttt ttggtttatt gcgtaacgcg ctgggaacgc cctccgcgcc
    10921 acctaaccag gtattgctgg ggctggcact gtttttgacc ttttttatta tgtcgccagt
    10981 gatcgacaaa atttatgtcg atgcgtacca gccattcagc gaagagaaaa tctcaatgca
    11041 ggaggcgctg gaaaaagggg cgcagccgct gcgtgagttt atgctgcgtc agacccgcga
    11101 ggcagattta gggttgtttg ccagactggc gaataccggc ccgttgcagg ggcctgaagc
    11161 tgtgccgatg cgcattttgc tcccggccta cgtgaccagc gagttgaaaa ccgcatttca
    11221 gataggcttc acgattttca tccctttttt gattatcgac ctggtgattg ccagcgtgtt
    11281 gatggcattg gggatgatga tggttccccc cgccaccatt gctctgccct ttaaactgat
    11341 gctgtttgta ctggtggatg gctggcaatt gctggtcggt tcgctggcgc agagctttta
    11401 cagctagaga ggcaaaatga cacctgaatc ggtcatgatg atgggaactg aagcgatgaa
    11461 agtcgcgctg gcactggcag ccccgctgtt gctggtagcg ctggtcacgg gccttatcat
    11521 cagtattttg caggcagcca cgcagattaa cgaaatgacg ctgtcgttta ttccgaaaat
    11581 catcgccgta tttatcgcca tcattattgc cggaccgtgg atgctcaatc tgttgctgga
    11641 ttacgtccgc accttgttca ctaacctgcc gtatatcatc gggtagcccg tacaatgatg
    11701 caggagacaa gcgatcaatg gctttcctgg ttaagcctgt acttctggcc gttactgcgc
    11761 gtgctggcgc tcatttctac cgcgccaatt ctgagcgaac gcagcgtgcc gaaacgggta
    11821 aaactgggtc tggcaatgat gatcacgttc gccattgccc catcattacc tgccaacgat
    11881 gttcctgttt tttcgttctt tgctctgtgg ctggccgtac agcagatcct gatcggcatt
    11941 gcgcttggtt ttaccatgca atttgccttt gccgctgtgc gaaccgctgg cgaaattatc
    12001 ggtctgcaaa tggggctgtc atttgcgaca tttgtcgatc ctggcagcca tctcaatatg
    12061 cccgttttag cgcgcatcat ggatatgctg gcattactgc tgttcctgac atttaacggt
    12121 catttatggt tgatttcact gctggtcgat acctttcaca ccctgccgat tggtagcgaa
    12181 ccgttgaaca gcaatgcgtt tctggcaccc accaaagcag ggagtttgat tttccttaac
    12241 gggctgatgc tggcgttacc gctcattact ctgttgctga ccctgaatct ggcattaggt
    12301 ttactgaatc gtatggcccc gcaattatcc atttttgtta ttggatttcc attaactctg
    12361 actgtcggca tctctttaat ggcggcatta atgccgttaa ttgcgccttt ttgcgaacat
    12421 ttattcagtg aaatttttaa tttgctggct gatattatta gtgaattacc attaatataa
    12481 ttccgtaacg tttatcatgc taccctaagg attatccgaa aaataatatc tgcgaacatc
    12541 ttccaggata ctcctgtagc gaaatatttg ttttaagctc actcacatat cgcaacattt
    12601 actttaagac aattccaggc aaattataca acactttacg ggatagtaag tccgcctgaa
    12661 aaattgcgag agtggcgcat taggtgaccc atgttgttcc gtttagtcat gatgaaatat
    12721 tcaggtaagg ggaattatcg ttacgcattg agtgagggta tgccatgtca acgattatta
    12781 tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt agaggggtta
    12841 aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct tgtgattcac
    12901 agcgtccttc agtggtgttt attaatgagg actgtttcat ccacgatgct tctaacagtc
    12961 agcgcatcaa gcacatcatt aatcaacatc ccaatacgtt atttatcgtt tttatggcaa
    13021 ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaaatttatt gatcagttct
    13081 aaatcgatta aaccggaatc tctcgacgat atccttggcg atattctgaa aaaagagaca
    13141 acgataacct cgtttttaaa tatgccgacg ttatcattga gccgaaccga atcgagtatg
    13201 ttgcgaatgt ggatggcagg tcagggaacc attcaaatct ccgaccaaat gaatatcaaa
    13261 gccaagaccg tttcatcgca taaaggtaat attaaacgta agatcaaaac gcataataaa
    13321 caggttatct accatgtcgt ccgactgacg gataatgtga ctaatggtat ttttgtcaac
    13381 atgcgctaac acattctgac tggtggtttc ccaccagtca ggctgaataa gattactctg
    13441 ctttctcaac aaagataccg tcctgatgcc ctgcttcatt aaagaaccag atgccgagcg
    13501 ggtagtcttc cagcgcaacc aggtacattg tgccttcact aaactcctca actgccagta
    13561 ccactccagg acgacgcgga ccgccatccg ttttgactgt tacccgatca ttcaccttca
    13621 tcgttttcct cctgtggctt tgtgccagtg tagaacaatt tcgtttttct ggcagcgcca
    13681 agcgcgcgcg agtgctgatt ttctcgacgg tctatactta agagatgcca ggcggactta
    13741 acgactggcg gcaacaacag agtaacggtt gcgaggaaag atgatgaaaa ccgcaaaagt
    13801 gtacagcgat accgcaaaac gtgaggtcag cgtcgatgtc gatgccctgc tggcggcgat
    13861 caatgaaatt agcgaaagcg aagttcattg atcgccgcca gaacgattct gaacacgtta
    13921 gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc gaactggata
    13981 ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa atcccgaccc
    14041 tgagggtgtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc gttacaccag
    14101 gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga aaatattcgc
    14161 gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc ccttggtggc
    14221 tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg ctgttttgag
    14281 aatcatcctg gtaaggggag tactgcgcga ccatgttttc aattcaacaa ccactactgg
    14341 tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg caaccggcag
    14401 ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt agcagtaaaa
    14461 ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta ccgctgattg
    14521 cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac ggttttccac
    14581 gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg ctacgcgaga
    14641 aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt gccgaatgga
    14701 cgggattaag ccgtagctag gcggcgctga cgcagctgca tgaggcgtcg gtaacgctaa
    14761 tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac gaactgggct
    14821 tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc ggaaaagatc
    14881 aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcagacgc ccaaccacac
    14941 ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat tacgcggtga
    15001 ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga tgaggatccg gcccgcgtct
    15061 ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat tttttctccg
    15121 cccgttaagc gttatcgctc gcgaataccc gattgcgccc ggcctgtttt gcgaggtaaa
    15181 gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt tcctcgctgc
    15241 tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc agcatctctt
    15301 tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc agattcgcgc
    15361 ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc tgcgcacgca
    15421 aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc tgatgaccaa
    15481 agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa aaaggatgtt
    15541 ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacagtgcg ccacggttat
    15601 ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc agaacataca
    15661 tgttgctgac catccggcga atcacatacc aggagaggag taacatggtg gtaaagagcg
    15721 cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg ccttcgctta
    15781 gcgtatggac gcgcgccagc acaccgtcga aatggtccag acgttcccag ctaacatagc
    15841 gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc agcaaggcca
    15901 gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag gtcaaaaatc
    15961 tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg gcgtttctta
    16021 aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac cagtagttat
    16081 tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg tgttccggtt
    16141 gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg ccgataaacc
    16201 agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac aaggtcggct
    16261 gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc ctgttcaacc atcgacgagg
    16321 agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt tcattgtcga
    16381 ggctttcatt ttcgcgagac aggaaattcc cctcgctgac taatgcatcc gatacgccat
    16441 tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg tcgcgatgct
    16501 gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc acgagagctt
    16561 cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga
    16621 gcgcgttggc tacgttttcc agatgattac gctagctgga gatataggca tcttccagca
    16681 ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata aagcagagat
    16741 taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc tggttttcca
    16801 tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat tctggcataa
    16861 cccggcaata gcgtaccgca aaacagcgat aattttataa tggtggcatg ataagacgcg
    16921 actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc tggtgttaat
    16981 gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa gatcggtttc
    17041 atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa ccataccgct
    17101 ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt cctcactaac
    17161 cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta gttgaagatg
    17221 gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca ttttcgctac
    17281 tgctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca gatttaaaac
    17341 agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgatatgtt caatagcgtg
    17401 atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca tcgccagcgt
    17461 gccgacaatc gataatgctt tcatcagcca aggcgcgata atcaataacc ctttacctaa
    17521 tgcctgcatc agcgcgctag atttttccgc cagccaatac cctaggtcat caatcttaac
    17581 gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc ctgaaagcac
    17641 cagcacctga ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg cgacgatttc
    17701 cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt caaacttcag
    17761 cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgctggat cttctttatg
    17821 tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga gaaacgcgcc
    17881 accaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga tcagcgccag
    17941 cggcaccaga atcactttat tgatcagcga tcctttcgcc acgccccaga ccacgggaag
    18001 ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataaat catcccctaa
    18061 tacaccggcg gttttcttcg ccgccagttt gcccatcaca gagatatcgt ccagcagtgt
    18121 ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc cctctcattt
    18181 ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt gcttcacagg
    18241 tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat gattttcacg
    18301 tttactatga accacctttt tatttccgcc gtgagggatt atgcgtttcc gccagttgtt
    18361 accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct attttgtcat
    18421 tcggattggc gtgggaagct ggcctccgtt aatgatggcg ggcgttcgat tccaggcagc
    18481 cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc cgctacgtcc
    18541 gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg gcatggtgac
    18601 ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtgattg caaccgtgcc
    18661 cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa acgcgcaaac tggaatgggt
    18721 gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa atttaagcgg
    18781 caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt ttggctcagt
    18841 ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg agatgctggc
    18901 ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt gaaaaactga cggcgctccc
    18961 ttccctgtca ggcttccttg cggtcggcta tctggcgctg tttggttcga ttatcgccat
    19021 taacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca gctacgccta
    19081 cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa cgctgtcgaa
    19141 gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca cgttgggaaa
    19201 atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat caagcgagta
    19261 aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc cactcttcca
    19321 gacgctcggt aagcgccgca tccgtcagct tctcgcgcct acgtaacgca cactcccaga
    19381 caatcagtac gcgccagccg agctcctgca agcgactgat atcgcggcga tcgcgttcaa
    19441 catttttacc tatcttctcc agccagaact cagttcgagt cgcaggcact ttaaacagat
    19501 agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga tattcatcaa
    19561 cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca aggccctgcc
    19621 cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca atcgcgcgca
    19681 tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc ttgctgacgc
    19741 aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc aaagaccggc
    19801 acgaccaccg agttgccgaa ctggcgatag gcctgagtgt ccgaaaccgg aatgcggaat
    19861 ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg cgttaaccga
    19921 cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt ggccatatcc
    19981 caaccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgagcaga cagcgtacgg
    20041 gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt accgcgcgcc
    20101 tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag gatatatttc
    20161 gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg gaaacattcg
    20221 ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa acccaccagc
    20281 acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat cgatgatttt cggatcgtcc
    20341 ggcccgttat cttctgcatc ggcaacgtca tagcccagtt cgtccagcgt ctgcatgatg
    20401 atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacgttttc gagcacaaac
    20461 atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt gccctgggta
    20521 tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc agccagcgaa
    20581 aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg ttgacgaata
    20641 tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat gtcgcggata
    20701 tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata agtgcgtacc
    20761 gcatgtttgt tccattcgct ggtaaacacg cactgtccac caatcgattc aaaaccacga
    20821 cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg cggatgatgt
    20881 tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact taaacggtgc
    20941 catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg attctcaccc
    21001 acaccattaa gctgcgccac cagcatttta acatcataaa tttgcagcag tttctccagc
    21061 attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt ttcctgcata
    21121 gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg tttagggcca
    21181 cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc ttcattctcc
    21241 cccgccagtt cggcaccgag cttcgccatc aaacagcgcc aagctcaatg cccctgaaac
    21301 ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac
    21361 gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc
    21421 atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcac ggagcaggcg
    21481 acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat ttttcgccag
    21541 gctcacgata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc
    21601 tgccgccagt ttctgtgccg tagcccagac acggcgaaaa tgacacaggt catgcgccgc
    21661 gtcctgatgt tgatgatggt ttttcaacca gttctcgaac tatgcctgcc agtgttgtaa
    21721 ctccatacgc gctccgttgc caaagagtct caaccttagc agtttttatt catagcgtct
    21781 gcaacgtgcg gaacgaggtc gggtgtacca tgcaatgccg cctaacaagg caccaaccag
    21841 ggccataaac agaaatccga ccaatgctcc taaccacttc cccgcggtag atcctaaatc
    21901 actttcaaat cccgacacgg gggcatcggg gatccggctc atcacccaga tgtatcgcgc
    21961 cattgcccag acgaaatagc cacaccatgt atagaaagcc c
//
LOCUS       DAZPFP010000046        21865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPFP010000046 DAZPFP010000000
VERSION     DAZPFP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.046"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            181..333
                     /gene="mokC"
                     /locus_tag="ACHUR4_001769"
     CDS             181..333
                     /gene="mokC"
                     /locus_tag="ACHUR4_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1455384.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(437..1567)
                     /gene="dnaJ"
                     /locus_tag="ACHUR4_001770"
     CDS             complement(437..1567)
                     /gene="dnaJ"
                     /locus_tag="ACHUR4_001770"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1455385.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1656..3572)
                     /gene="dnaK"
                     /locus_tag="ACHUR4_001771"
     CDS             complement(1656..3572)
                     /gene="dnaK"
                     /locus_tag="ACHUR4_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1455386.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3949..4353
                     /locus_tag="ACHUR4_001772"
     CDS             3949..4353
                     /locus_tag="ACHUR4_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1455387.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4379..5092
                     /gene="msyB"
                     /locus_tag="ACHUR4_001773"
     CDS             4379..5092
                     /gene="msyB"
                     /locus_tag="ACHUR4_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1455388.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5241..5807
                     /gene="satP"
                     /locus_tag="ACHUR4_001774"
     CDS             5241..5807
                     /gene="satP"
                     /locus_tag="ACHUR4_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1455389.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5842..6429)
                     /gene="mog"
                     /locus_tag="ACHUR4_001775"
     CDS             complement(5842..6429)
                     /gene="mog"
                     /locus_tag="ACHUR4_001775"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1455390.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6544..7497)
                     /gene="tal"
                     /locus_tag="ACHUR4_001776"
     CDS             complement(6544..7497)
                     /gene="tal"
                     /locus_tag="ACHUR4_001776"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1455391.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7776..9206
                     /locus_tag="ACHUR4_001777"
                     /pseudo
     CDS             7776..9206
                     /locus_tag="ACHUR4_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9276..10052
                     /gene="yaaA"
                     /locus_tag="ACHUR4_001778"
     CDS             9276..10052
                     /gene="yaaA"
                     /locus_tag="ACHUR4_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1455392.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10205..10501)
                     /locus_tag="ACHUR4_001779"
     CDS             complement(10205..10501)
                     /locus_tag="ACHUR4_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1455393.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10715..12001)
                     /gene="thrC"
                     /locus_tag="ACHUR4_001780"
     CDS             complement(10715..12001)
                     /gene="thrC"
                     /locus_tag="ACHUR4_001780"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1455394.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(12002..12934)
                     /gene="thrB"
                     /locus_tag="ACHUR4_001781"
     CDS             complement(12002..12934)
                     /gene="thrB"
                     /locus_tag="ACHUR4_001781"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1455395.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12936..15398)
                     /gene="thrA"
                     /locus_tag="ACHUR4_001782"
     CDS             complement(12936..15398)
                     /gene="thrA"
                     /locus_tag="ACHUR4_001782"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1455396.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15424..15543)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15479..15544)
                     /gene="thrL"
                     /locus_tag="ACHUR4_001783"
     CDS             complement(15479..15544)
                     /gene="thrL"
                     /locus_tag="ACHUR4_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1455397.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15758..16444)
                     /locus_tag="ACHUR4_001784"
     CDS             complement(15758..16444)
                     /locus_tag="ACHUR4_001784"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1455398.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16844..16984)
                     /gene="yjjY"
                     /locus_tag="ACHUR4_001785"
     CDS             complement(16844..16984)
                     /gene="yjjY"
                     /locus_tag="ACHUR4_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1455399.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17080..17796
                     /gene="arcA"
                     /locus_tag="ACHUR4_001786"
     CDS             17080..17796
                     /gene="arcA"
                     /locus_tag="ACHUR4_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1455400.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17855..19207)
                     /gene="creD"
                     /locus_tag="ACHUR4_001787"
     CDS             complement(17855..19207)
                     /gene="creD"
                     /locus_tag="ACHUR4_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1455401.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19265..20689)
                     /gene="creC"
                     /locus_tag="ACHUR4_001788"
     CDS             complement(19265..20689)
                     /gene="creC"
                     /locus_tag="ACHUR4_001788"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1455402.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20689..21378)
                     /gene="creB"
                     /locus_tag="ACHUR4_001789"
     CDS             complement(20689..21378)
                     /gene="creB"
                     /locus_tag="ACHUR4_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1455403.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21391..21864)
                     /gene="creA"
                     /locus_tag="ACHUR4_001790"
     CDS             complement(21391..21864)
                     /gene="creA"
                     /locus_tag="ACHUR4_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1455404.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         5200 a         5850 c         5344 g         5471 t
ORIGIN      
        1 atgggttaaa gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt
       61 agctgaacgg cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc
      121 tatatgctga acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca
      181 atgaagcagc ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg
      241 gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag
      301 gttgctgttt tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc
      361 actttacagg tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg
      421 caggcttttg gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga
      481 agctctttga gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct
      541 cttgcagcag ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc
      601 acagcaaatc accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga
      661 atagcttacc ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg
      721 gtacttcgat ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca
      781 ggttgttgcc ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat
      841 cgcctgccgg tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc
      901 cagtgtccac ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat
      961 gaccatgaca tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac
     1021 aggtctgctg tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg
     1081 tcggacaggt ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact
     1141 cttccagagt cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga
     1201 gctccatgtt atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc
     1261 caaaaatatc gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac
     1321 caccgccgcc catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac
     1381 gtttttgcga gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg
     1441 cctctttgtc accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct
     1501 ttctgatttc acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct
     1561 tagccatctt ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc
     1621 gcccgtgtca gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca
     1681 aattcagcgt cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca
     1741 gtctgctgct gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc
     1801 agttcctgca ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt
     1861 gcagtcagcg cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct
     1921 tcaacctgct tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc
     1981 tcttcaaact tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt
     2041 tcatcttcgt tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt
     2101 ttatctttcg cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc
     2161 tgcggcatgc cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg
     2221 ttatcagccg cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg
     2281 tcttcagcgg tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc
     2341 agcgtcgtca tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc
     2401 agcagtacgt cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct
     2461 acagcttcgt ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc
     2521 ttctgaacca ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat
     2581 acggacaggc cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga
     2641 tcttcaacca ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg
     2701 gtcgcgtctg cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg
     2761 attttcgctt tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg
     2821 tcaatgccct gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag
     2881 tcttcaccac ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg
     2941 tcaacttcgt cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc
     3001 gcgatagtac ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt
     3061 tcgttgatga tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga
     3121 cgctgagcat cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc
     3181 aggtaatctt cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt
     3241 gccattttct ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg
     3301 aacggcatga tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg
     3361 cgtttaatcg caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga
     3421 ccaaccagag tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct
     3481 tcggcgttct ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta
     3541 gtacccaggt cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca
     3601 tcatgtggtt gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat
     3661 ttttttgcag actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca
     3721 agggggagat gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca
     3781 agacgtaaaa ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca
     3841 tcaaaaaatt gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta
     3901 aactagtggg tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt
     3961 tttacgattt ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac
     4021 gaccataaac tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg
     4081 aaacttcctg tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta
     4141 cagttaagtg gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat
     4201 attccttcag aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac
     4261 gacaataaac gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat
     4321 atggccacgc tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat
     4381 gaatgttaat tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca
     4441 gttggcaaat ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag
     4501 catactgatg cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg
     4561 caactggcag ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa
     4621 actgcgcgga cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa
     4681 gctgaaagcc gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt
     4741 tctgcgtaat acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt
     4801 cgatgccagg gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt
     4861 ggcaaaaggt gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc
     4921 agcaatgagc gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg
     4981 cgcagcatta aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc
     5041 cgtactgcaa atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa
     5101 accggggaaa attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt
     5161 attatgccgc gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg
     5221 atgatgattt ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc
     5281 tgatgggctt cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc
     5341 tggacggtat tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg
     5401 gtctgctgga gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt
     5461 ctttctggct gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc
     5521 caaatgcaca gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt
     5581 tcttcggcac tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc
     5641 tgtttgccct gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg
     5701 gctggattgg tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga
     5761 acgagcagtt tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc
     5821 cccccgctcg gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa
     5881 tgcagcaacc acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta
     5941 cggtacgctg gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc
     6001 cagcgtctct ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg
     6061 aatcacgccc acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg
     6121 catctgttca ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt
     6181 tacgtcacga cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc
     6241 atccaccagc tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt
     6301 ttccagttca aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc
     6361 tttatcctga taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa
     6421 agtattcatc aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa
     6481 gggagtgaca gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa
     6541 tgattacagc agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg
     6601 gataccttcc gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc
     6661 ggactcagtg atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc
     6721 gatagcccct tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc
     6781 acagcctgcc agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt
     6841 ttcataaccg tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc
     6901 ttctgccgga gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc
     6961 aacaaacggc gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga
     7021 gaacagcagg gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat
     7081 accctgccag gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt
     7141 gtagagtttg atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg
     7201 cgcatcaact tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt
     7261 tactgccagt ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt
     7321 cgcccaggcg acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag
     7381 aatgagagaa gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc
     7441 cccagtgtcg gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat
     7501 agtatttctc tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac
     7561 gacgcaacgt cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat
     7621 ttctgacact gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca
     7681 gaagttatca agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag
     7741 gaaagtcatc gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta
     7801 acagcgtcct ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca
     7861 cttttcgcac cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa
     7921 atagcattca tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg
     7981 cggcgcgcgt gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac
     8041 caggtgccgt cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg
     8101 aatgttccct tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac
     8161 cggcatggta tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct
     8221 ttttgctcat cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag
     8281 ccctgagttt ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg
     8341 ctctgctggc aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc
     8401 cgttgatggc gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc
     8461 aacttcccca cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag
     8521 gcggcgcggc gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt
     8581 tttccaatga ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc
     8641 ctccgcatcc ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg
     8701 tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc
     8761 cgctggaagg tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg
     8821 ctgaatttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca
     8881 tttatgccga aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt
     8941 tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt
     9001 ggcaactggc agatatcata atggcctgca tggctattac caatttgacc gccattttac
     9061 tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg
     9121 tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg
     9181 cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg
     9241 ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga
     9301 aaacgcttga ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag
     9361 acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc
     9421 tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc
     9481 cagatttcac gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca
     9541 ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc
     9601 gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc
     9661 tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag
     9721 atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta
     9781 atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta
     9841 tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta
     9901 agaaagcacg cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc
     9961 aactgactgg ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg
    10021 aactggtgtt taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg
    10081 aacgccttat ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc
    10141 ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg
    10201 tcatttagcg gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct
    10261 tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat
    10321 aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat
    10381 tatcacgatc gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg
    10441 ctgccatggg agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca
    10501 caggttactc cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga
    10561 acgacggttg ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc
    10621 tcctttttcc ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa
    10681 gcaaacccgg cctgattgag ataatgaata gattttactg atgattcatc atcaatttac
    10741 gcaacgcaga aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg
    10801 ccagctcttt tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt
    10861 tcgccggatg cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca
    10921 gcgcacgata cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac
    10981 gcattgtctg ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc
    11041 agattttgcg gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca
    11101 tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac
    11161 gtggcacggt atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg
    11221 acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca
    11281 gctggttgcg cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg
    11341 ccagcaaacg gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt
    11401 cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg
    11461 caacagtttc gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt
    11521 tgcctcgtgg atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca
    11581 ctgcagctcc ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa
    11641 tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc
    11701 cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg
    11761 caaacgccgc gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg
    11821 ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag
    11881 tcaggctgaa ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct
    11941 gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca
    12001 tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt
    12061 cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct
    12121 tgtcacatag agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga
    12181 tttccgcgac cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt
    12241 cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt
    12301 gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg
    12361 gtaaaatagc ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca
    12421 gccactcatc aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact
    12481 gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc
    12541 cttccagctc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt
    12601 cattcatcgc catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga
    12661 tcggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa
    12721 aacgctccca gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa
    12781 agcgtccgag tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga
    12841 gcaatgcacc atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat
    12901 tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg
    12961 gtacgtagca gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg
    13021 cgcagcacca acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca
    13081 tttttcactt tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca
    13141 ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc
    13201 acgcgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca
    13261 ccctcggcgt taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt
    13321 tcacgtcccg tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga
    13381 tcatctcgcg ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag
    13441 aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg
    13501 gagaacttca ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt
    13561 aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa
    13621 cgcaactgat gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg
    13681 tggaaacctt cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag
    13741 tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga
    13801 ttaaacggct ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc
    13861 acactagtga gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta
    13921 ttcttcagcc agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg
    13981 acgccaatca caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg
    14041 cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat
    14101 ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc
    14161 gagatcccac gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc
    14221 gtcaccgcca gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt
    14281 gcccgttcag ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa
    14341 gatgattgcg taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc
    14401 gccgccatgc caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta
    14461 ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc
    14521 gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg
    14581 atgggggtaa tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct
    14641 tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta
    14701 tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc
    14761 accgccgcgg agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta
    14821 ccggcggtga aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata
    14881 cggcgggtgg actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt
    14941 ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata
    15001 atggcgatcg acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac
    15061 tgccccaaca aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg
    15121 aaagttttca attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt
    15181 tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt
    15241 ttttcaatca tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc
    15301 acctgcccct gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca
    15361 tttgccactg atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg
    15421 gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc
    15481 agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt
    15541 tcatggatgt tgtgtactct gtaattttta tctgtttgtg cgctatgcct atattggtta
    15601 aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca
    15661 gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc
    15721 agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa
    15781 tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt
    15841 gctgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca
    15901 tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg
    15961 gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga
    16021 gcgaaggata atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt
    16081 cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag
    16141 atttttcctc taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc
    16201 gactacgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg
    16261 ggaaaacttt aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg
    16321 gctccaggtg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg
    16381 cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac
    16441 gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt
    16501 acgcggtgcg aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt
    16561 aaaaaaggct aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat
    16621 atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca
    16681 cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc
    16741 atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc
    16801 aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa
    16861 tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca
    16921 agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag
    16981 tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta
    17041 cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt
    17101 atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc
    17161 tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac
    17221 atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt
    17281 gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc
    17341 gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg
    17401 cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc
    17461 agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac
    17521 agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc
    17581 gccatgcttc acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag
    17641 aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt
    17701 attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt
    17761 gaaggttatc gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa
    17821 cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt
    17881 ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat
    17941 tacgagtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca
    18001 gcgcgctatc ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga
    18061 gggtaaacaa catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca
    18121 ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa
    18181 gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg
    18241 ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa
    18301 tcagcagaat ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg
    18361 tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac
    18421 gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc
    18481 tagcttcccg tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg
    18541 ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta
    18601 aattcagtgc catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga
    18661 tatgcacgcc ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc
    18721 cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa
    18781 taaagggctt gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga
    18841 aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc
    18901 gttcttccac gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa
    18961 aacttcgttt ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag
    19021 gaatagcgat aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat
    19081 cttccacatc gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg
    19141 gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt
    19201 tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt
    19261 gaagctatgt gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt
    19321 tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca
    19381 gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg
    19441 acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct
    19501 gatccacttc ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat
    19561 ccagtaaatt ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg
    19621 tgggcataac atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga
    19681 cgcggcgaaa taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca
    19741 gtcttgcctg gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa
    19801 tgttgtcagt aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg
    19861 cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct
    19921 caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac
    19981 gcagctcgct actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag
    20041 catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca
    20101 ccagtgcaat ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa
    20161 tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc
    20221 cgcccataat cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca
    20281 acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat
    20341 aatcctgtcc aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca
    20401 tatagacatg atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc
    20461 gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca
    20521 aatcgggacg cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg
    20581 ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca
    20641 ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct
    20701 caggctatat cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg
    20761 cagcttggca cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc
    20821 atcttcccac acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag
    20881 taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct
    20941 gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt
    21001 cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga
    21061 aaacggttta gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc
    21121 ttcacttcgg gccgtcagga acagtacagg taacgccgga tagagcgcca gtaactggcg
    21181 gcacaattca aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac
    21241 ctgctggcga gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc
    21301 ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca
    21361 gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc
    21421 gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag
    21481 cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt
    21541 gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc
    21601 aatcggcccg acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa
    21661 tcccccttta ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc
    21721 gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt
    21781 gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga
    21841 caggatcaaa tgcttgtatt tcata
//
LOCUS       DAZPFP010000047        21512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPFP010000047 DAZPFP010000000
VERSION     DAZPFP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.047"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUR4_001791"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUR4_001791"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1455405.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUR4_001792"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUR4_001792"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1455406.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUR4_001793"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUR4_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1455407.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUR4_001794"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUR4_001794"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1455408.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUR4_001795"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUR4_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUR4_001796"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUR4_001796"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1455409.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUR4_001797"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUR4_001797"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1455410.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUR4_001798"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUR4_001798"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1455411.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUR4_001799"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUR4_001799"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1455412.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUR4_001800"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUR4_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1455413.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUR4_001801"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUR4_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1455414.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUR4_001802"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUR4_001802"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1455415.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUR4_001803"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUR4_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1455416.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUR4_001804"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUR4_001804"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1455417.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUR4_001805"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUR4_001805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1455418.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUR4_001806"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUR4_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1455419.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHUR4_001807"
     CDS             complement(18170..18565)
                     /locus_tag="ACHUR4_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1455420.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUR4_001808"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUR4_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1455421.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHUR4_001809"
     CDS             19481..20074
                     /locus_tag="ACHUR4_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1455422.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUR4_001810"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUR4_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1455423.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHUR4_001811"
     CDS             20794..>21512
                     /locus_tag="ACHUR4_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1455424.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZPFP010000048        21505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPFP010000048 DAZPFP010000000
VERSION     DAZPFP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.048"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHUR4_001812"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHUR4_001812"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1455425.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHUR4_001813"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHUR4_001813"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1455426.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHUR4_001814"
     CDS             complement(3121..3420)
                     /locus_tag="ACHUR4_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1455427.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHUR4_001815"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHUR4_001815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1455428.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHUR4_001816"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHUR4_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR1455429.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHUR4_001817"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHUR4_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1455430.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHUR4_001818"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHUR4_001818"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1455431.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHUR4_001819"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHUR4_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHUR4_001820"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHUR4_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1455432.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHUR4_001821"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHUR4_001821"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR1455433.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHUR4_001822"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHUR4_001822"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR1455434.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHUR4_001823"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHUR4_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACHUR4_001824"
     CDS             complement(13973..14797)
                     /locus_tag="ACHUR4_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR1455435.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHUR4_001825"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHUR4_001825"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1455436.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHUR4_001826"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHUR4_001826"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR1455437.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACHUR4_001827"
     CDS             complement(18729..19202)
                     /locus_tag="ACHUR4_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1455438.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHUR4_001828"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHUR4_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR1455439.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHUR4_001829"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHUR4_001829"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR1455440.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21505
                     /locus_tag="ACHUR4_001830"
     CDS             21362..>21505
                     /locus_tag="ACHUR4_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR1455441.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5404 a         5489 c         5277 g         5335 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgctgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaa
//
LOCUS       DAZPFP010000049        20780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPFP010000049 DAZPFP010000000
VERSION     DAZPFP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.049"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHUR4_001831"
     CDS             <1..579
                     /locus_tag="ACHUR4_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1455442.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHUR4_001832"
     CDS             597..1235
                     /locus_tag="ACHUR4_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1455443.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUR4_001833"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUR4_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1455444.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUR4_001834"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUR4_001834"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1455445.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUR4_001835"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUR4_001835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1455446.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUR4_001836"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUR4_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1455447.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHUR4_001837"
     CDS             complement(5552..6613)
                     /locus_tag="ACHUR4_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1455448.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHUR4_001838"
     CDS             complement(6610..8007)
                     /locus_tag="ACHUR4_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1455449.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHUR4_001839"
     CDS             8163..9161
                     /locus_tag="ACHUR4_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1455450.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUR4_001840"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUR4_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHUR4_001841"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHUR4_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUR4_001842"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUR4_001842"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1455451.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUR4_001843"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUR4_001843"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1455452.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHUR4_001844"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHUR4_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHUR4_001845"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHUR4_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHUR4_001846"
     CDS             complement(16089..17141)
                     /locus_tag="ACHUR4_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1455453.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHUR4_001847"
     CDS             complement(17172..18014)
                     /locus_tag="ACHUR4_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1455454.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHUR4_001848"
     CDS             complement(18001..18882)
                     /locus_tag="ACHUR4_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1455455.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHUR4_001849"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHUR4_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHUR4_001850"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHUR4_001850"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR1455456.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5669 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttgccctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZPFP010000050        20706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPFP010000050 DAZPFP010000000
VERSION     DAZPFP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.050"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..137)
                     /locus_tag="ACHUR4_001851"
     CDS             complement(<1..137)
                     /locus_tag="ACHUR4_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1455457.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     I"
     gene            complement(315..1964)
                     /gene="actP"
                     /locus_tag="ACHUR4_001852"
     CDS             complement(315..1964)
                     /gene="actP"
                     /locus_tag="ACHUR4_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1455458.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1961..2275)
                     /locus_tag="ACHUR4_001853"
     CDS             complement(1961..2275)
                     /locus_tag="ACHUR4_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR1455459.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2475..4433)
                     /gene="acs"
                     /locus_tag="ACHUR4_001854"
                     /pseudo
     CDS             complement(2475..4433)
                     /gene="acs"
                     /locus_tag="ACHUR4_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4826..6262
                     /gene="nrfA"
                     /locus_tag="ACHUR4_001855"
     CDS             4826..6262
                     /gene="nrfA"
                     /locus_tag="ACHUR4_001855"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1455460.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6307..6873
                     /gene="nrfB"
                     /locus_tag="ACHUR4_001856"
     CDS             6307..6873
                     /gene="nrfB"
                     /locus_tag="ACHUR4_001856"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1455461.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6870..7541
                     /gene="nrfC"
                     /locus_tag="ACHUR4_001857"
     CDS             6870..7541
                     /gene="nrfC"
                     /locus_tag="ACHUR4_001857"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1455462.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7538..8494
                     /gene="nrfD"
                     /locus_tag="ACHUR4_001858"
     CDS             7538..8494
                     /gene="nrfD"
                     /locus_tag="ACHUR4_001858"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1455463.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8514..10232
                     /locus_tag="ACHUR4_001859"
     CDS             8514..10232
                     /locus_tag="ACHUR4_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1455464.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10225..10608
                     /gene="nrfF"
                     /locus_tag="ACHUR4_001860"
     CDS             10225..10608
                     /gene="nrfF"
                     /locus_tag="ACHUR4_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1455465.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10605..11201
                     /gene="nrfG"
                     /locus_tag="ACHUR4_001861"
     CDS             10605..11201
                     /gene="nrfG"
                     /locus_tag="ACHUR4_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1455466.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11543..12856
                     /gene="gltP"
                     /locus_tag="ACHUR4_001862"
     CDS             11543..12856
                     /gene="gltP"
                     /locus_tag="ACHUR4_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1455467.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12934..13623)
                     /gene="yjcO"
                     /locus_tag="ACHUR4_001863"
     CDS             complement(12934..13623)
                     /gene="yjcO"
                     /locus_tag="ACHUR4_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1455468.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13717..15864)
                     /gene="fdhF"
                     /locus_tag="ACHUR4_001864"
                     /pseudo
     CDS             complement(13717..15864)
                     /gene="fdhF"
                     /locus_tag="ACHUR4_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(16062..17528)
                     /gene="mdtP"
                     /locus_tag="ACHUR4_001865"
     CDS             complement(16062..17528)
                     /gene="mdtP"
                     /locus_tag="ACHUR4_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1455469.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17525..19567)
                     /gene="mdtO"
                     /locus_tag="ACHUR4_001866"
     CDS             complement(17525..19567)
                     /gene="mdtO"
                     /locus_tag="ACHUR4_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1455470.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19567..20598)
                     /gene="mdtN"
                     /locus_tag="ACHUR4_001867"
     CDS             complement(19567..20598)
                     /gene="mdtN"
                     /locus_tag="ACHUR4_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1455471.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20617..>20706)
                     /locus_tag="ACHUR4_001868"
     CDS             complement(20617..>20706)
                     /locus_tag="ACHUR4_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455472.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4647 a         5459 c         5666 g         4934 t
ORIGIN      
        1 tcaattaacg tgtttttatg atgtttgctg taattatctg attttgtttt ctttgtatgt
       61 gatgaacttg tgatagagtg gttatcgtta atctggcaaa gagggaaaaa catattattt
      121 aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg catattctct
      181 gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta
      241 atattatggg aaaaggtgat gcatgttgga gaggaagcgt attccccggt caggcgaccg
      301 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
      361 ataaactgcg cgcggaacag ttcacgctcg cgcgcccctt ccgctgagtt atcggttgcc
      421 gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
      481 gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tagtcgggcc gaggatcatc
      541 agcaccactg cggtaatcag ccccagccag ccgcccagca tcgcgccacg ggtagtcagt
      601 ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac
      661 gcaaggccta ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatcgca
      721 atcacgccga ggatcagtac ggtgattttt gatacccgca gctcttcacg ttcggtcgcg
      781 ccttttttga agacgttagc gtacaagtca tgcgaaaccg ccgatgcgcc cgccagcgtc
      841 agatccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa accgaggaat
      901 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
      961 tggcccgccg cgtctttata ttccggattc gcaccaacaa gcatgatcgc gccgaagccg
     1021 ataataaagg tcagaatata gaagtagccc ataaatccgg tggcgtagaa cacgctctta
     1081 cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc
     1141 gtaccaaaca tcagtcccag ccccagagag agcgcggaga tcggatcttt caccagtccg
     1201 cccggtttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct gaacagattg
     1261 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     1321 aacagcacag ctttgataat ttgtacccag gtggtcgcca gcatgccgcc gaacaggacg
     1381 tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc
     1441 agctcgatca gtttacctgc gcctaccatc tgagcgataa ggtaaagcgc caccactacc
     1501 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacgata agaggccaca
     1561 tcggcagagg tgtagcgtcc caggttacgc agacgttcgg caatcagaaa cagaatgatt
     1621 ggccagccca ccaggaagcc caacgagtag atcagcccgt catagccgga ggtaaacacc
     1681 agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg
     1741 ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
     1801 tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     1861 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
     1921 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     1981 gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
     2041 tgagaacaaa ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg
     2101 tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     2161 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     2221 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag
     2281 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     2341 cgctcgggta acccttgccg aatgtaggcc ggataaggcg tttacgccgc atccggcaat
     2401 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac cgattcgcat
     2461 ccggcaattg tgggttacga tggcatcgcg atagcctgct tctcttcaag cagcttctcg
     2521 accacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc gcccgccgca
     2581 attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag ggagtcggtc
     2641 cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca gttgcggact
     2701 tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacata ggcgtagatc
     2761 gcctgacctt taatattgtg cggaatacct actacggcgg cttcggcaat cttcggatgc
     2821 gccaccagcg ccgactctat ctctgccgtc cccagacggt gaccggagac gttcagcacg
     2881 tcgtccacac gcccggtgat ccagtaatag ccatcttcat cgcgacgcgc gccgtcgccg
     2941 ctgaaataca tatttttgaa ggtggaaaaa taggtctgct caaaacgttc gtgatcgcca
     3001 aacagcgtac gcgcctgacc aggccaggag tcggtgatca ccaggctacc ttcggtagcc
     3061 ccttccagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa gaacggacgt
     3121 gttgccgaac cggctttcag ctcggtagcg ccaggcagcg gggtgatcat gaaaccgccg
     3181 gtttcggtct gccaccaggt atcgaccacc ggacatttct cgttgccgat ttttttccag
     3241 taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat gcgtagcgac
     3301 gaacggtcgg tgccttcaat cgctttatcg ccttccgcca tcagcgcgcg gatcgccgtg
     3361 ggtgcggtat agagaatatt gacctgatgc ttgtccacca cctgcgccat acgggcaggc
     3421 gtcggccagt tcggtacgcc ttcaaacatc agcgtggttg cgccgcaggc cagcgggccg
     3481 tacagcaaat aactgtgtcc ggtaacccag cccacatcgg cggtgcacca gtagatatca
     3541 cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag ataaccgccg
     3601 gtagtgtgca gtacgccttt tggttttccg gtagaaccgg aggtatagag aataaacagc
     3661 ggatcttcgg cgttcatctc ttccgcctag tgctgatcgc tggcttgctc aaccaggtcg
     3721 tgccaccaca ggtcgcgccc ttcctgccag tcgatgtttc cgccagtacg cttcagtacc
     3781 accacatgct ctacgctggt gacgttcggg ttttttagtg cgtcatcaac gtttttcttc
     3841 agcggaatac tacgcccggc gcgcacgcct tcgtcggaag tgatcaccag tcgtgagttg
     3901 gaatcaataa tgcgcccggc aacggcttcc ggcgagaagc cgccgaaaat caccgaatgc
     3961 atcgcgccaa tgcgggcgca ggccagcatc gcaaccgcgg cttccggcac catcggcata
     4021 taaatcgcca ccacatcacc ttttttaatg cccagctcga gcagggtatt ggcgaagcgg
     4081 cagacgtcgc ggtgcagctc tttatagctg atatgtttgc tctggctggc gtcgtcgcct
     4141 tcccagatga tggcggtgcg atcgccgttt tcttgcagat ggcggtcaag gcagtttgcc
     4201 gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg ggcaaaggag
     4261 gtatttttca ccttctggta cggtttgatc cagtcgagaa tttttccctg ttcgccccag
     4321 aaggtatcag gtacgttaat agattgttga tacatcgcct cgtactgctg agggtttatc
     4381 aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttggct catgcttttg
     4441 ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg gtattggcag
     4501 ttttgtttag tatttgggcg acagatcacg caaaagtaga atggtgcaaa taaacggcag
     4561 ggtaattttt gaaggtcacc aacaaaagtt gattaattct ttgaggaaca tgcagttatg
     4621 catgctgttg aaaaagagga agatactgac taactctaaa gtggtatttc acatgcactt
     4681 acaattgatt aaagacaaca ttcacagtgt ggttatttgt tacacatagg ggtgagcaat
     4741 gtcatgacag tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc cattgcaaca
     4801 atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt
     4861 cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc
     4921 cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcacgatca
     4981 atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga
     5041 tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagcctcg
     5101 tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa
     5161 cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg
     5221 tctgatccag aaagacggcg aagatggcta cttccacggt aaatgggcgc gcggcggccc
     5281 ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc
     5341 caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat
     5401 tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg
     5461 ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg
     5521 catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa
     5581 ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc
     5641 gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa
     5701 cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca
     5761 gacttgtgcg aactgccata cccaggataa agctgccttg caaaaagtgg tcgcggaacg
     5821 taagcaatcg attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt
     5881 cgaagcgaaa gcggcgctgg atgcaggcgc gacggaagcc gaaatgaagc cgattcagga
     5941 cgatatccgt catgcccagt ggcgttggga tctggcgatt gcttcccacg gtattcatat
     6001 gcacgcaccg gaagaaggtc tacggatgct cggtacggcg atggataaag cggcggatgc
     6061 gcgcaccaaa ctggcgcgcc tgctggcgac caaaggtatc acccatgaaa tccagatccc
     6121 ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa
     6181 ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagagc aggcacgtaa
     6241 aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga
     6301 gtgaatatga gcgtattacg ttcgttatta actgctgggg tgctggcgtc gggcctgttg
     6361 tggagcctga acggaattac cgctacccct gcggtgcagg catctggtga tcgctacgaa
     6421 gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa
     6481 ggtatgcatg gcaaacatgc ttccgttatc aacccgaata acaaattgcc ggtcacctgc
     6541 accaactgtc acggccaagc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc
     6601 tttaacgagc cgatgtataa ggtcggagag cagaacagcg tctgtatgtc ctgtcatctg
     6661 ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc
     6721 gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga
     6781 cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg
     6841 gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg
     6901 gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga
     6961 atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg
     7021 cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga
     7081 ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt
     7141 cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc
     7201 gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact
     7261 gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca
     7321 aatgcgattt ctgtcgtaag accaatttgc aggccggtaa gcttcccgcg tgcgttgaag
     7381 cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc
     7441 aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc
     7501 tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca
     7561 ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc
     7621 tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc
     7681 agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct
     7741 gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta
     7801 cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct
     7861 ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt
     7921 gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact
     7981 ggaaacgctg atgctggtgt tggcggtgct gctgggggct tataccggct ttctgctgtc
     8041 ggcgctgaaa tcgtatccgt tcctcaataa cccgagcctg ccggtgctgc tcctcttctc
     8101 cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa
     8161 cccgcattcc acggaagcgc actttgttca ccggatggaa atccccgtgg tatggggtga
     8221 aatcttcctg ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg gtaaagttcg
     8281 tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc
     8341 cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat
     8401 tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt
     8461 cattctctac gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg
     8521 tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga
     8581 ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt
     8641 gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt
     8701 gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac agcctgctgt
     8761 cgtgggaact caaactggcg gcggtgtggg gcggtcatga aggttcgctg ctgctttggg
     8821 tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaagcgatc
     8881 cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg ctactgtttg
     8941 tggtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc
     9001 tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct
     9061 atggcggttt gatggtagcg gcgagcgtgg cgctggcgag tctactgcgc ggcgagtttg
     9121 atgcggccag cgcccgaatt tgctggcgct gggcgttacc tggctggagt gcattaacgg
     9181 cggggatcat cctcggttcc tggtgggcct actgcgaact cggctggggc ggctggtggt
     9241 tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc
     9301 acagtttgtc cctgacacgc cagcggggga ttttccgcca ctggtcgctg ttgctggcga
     9361 tagttactct gatgctgtcg ctgctgggca ccttaattgt ccgttctggc attctggttt
     9421 cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac
     9481 tgattagcct tgcgtctttg gctctgtatg gctggcgagc gcgggacggt ggcccggcgg
     9541 tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg
     9601 cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggc ctgctgggct
     9661 ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggtctgt
     9721 tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg
     9781 cgctggtagc tcatgctggc gtgctgttat ttgccgcggg gatcgtggtt tccagtgtca
     9841 gtcgtcagga gaacagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca
     9901 ccttccgttt tgagcgactc gatctgcaag ccagaggcaa ttacaccagc gaaaaagcga
     9961 tagtggcgct gtttgaccat cagcaacgca ttggtgaatt aacgccggag cggcgttttt
    10021 atgaagcacg ccgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact
    10081 ggtatgcggt catgggtgaa aaaaccagag cggatcgtta cgcttttcgt ttgtatgtac
    10141 aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa
    10201 gcggatggcg ggggaagaag cgcgatgaat aaagggcttc tcacgctact gctgttattt
    10261 acctgttttg cgcgcgctca ggttgtagac acctggcaat tcgccaatcc gcaacaacag
    10321 caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta
    10381 ctcgaatcca atgcgccggt ggcggtcagt atgcgccatc aggtttatag catggtgtcg
    10441 gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt
    10501 cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg
    10561 ttattgctga tggcactgat cctctggcga gtgagggcta agcgatgaaa cagcctcaaa
    10621 taccggtgaa aatgctgaca acactcacga tattgatggt attcctctgt gtcggcagtt
    10681 atctgttaag cccaaaatgg caggcggtac gtgcggagta tcagcgtcag cgcgatccgc
    10741 tacatcagtt tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa
    10801 tccgcgctaa tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc
    10861 aaaacgatta cagcaattcg ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga
    10921 acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga
    10981 ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg
    11041 ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac
    11101 tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt
    11161 cgattaatat ggcaaaactg ttgcagcgga gatcggatta atcatctggt gatgaagatt
    11221 atgtcattga gctgcataaa aaataatcga atgaacatat gccaaaaata atcaccaatc
    11281 agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc
    11341 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
    11401 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
    11461 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
    11521 ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct ggcagattct
    11581 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
    11641 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
    11701 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
    11761 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
    11821 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
    11881 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
    11941 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
    12001 gtcgatggcg aaaggcgaaa tgctgccgat catctttttc tcggtgctgt ttggtctggg
    12061 gctttcttcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
    12121 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
    12181 gctgattgcg gtgacggtgg ctaactttgg tttctcgtct ctgtggccac tggcgaaact
    12241 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
    12301 acgtctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
    12361 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
    12421 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
    12481 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
    12541 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
    12601 gattgctggc gtggctggcg tgtcgtttgt ggtgttgctg gcaacgctgg gtagcgtagg
    12661 tattccgctg gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
    12721 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
    12781 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
    12841 aactgcggat caataattga agattgccgg ggatatcccc cggcaatgtg tggatgcctg
    12901 atgcgacgct tgccgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
    12961 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
    13021 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
    13081 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
    13141 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
    13201 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
    13261 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
    13321 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
    13381 cggattggtg attttgatct gcgccaacag cgcgcaggca tcagcatcac cgctgtcggc
    13441 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
    13501 aagaaaatat tgtgcacgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
    13561 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
    13621 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
    13681 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
    13741 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcgga
    13801 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
    13861 gctttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
    13921 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
    13981 cactcaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
    14041 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
    14101 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
    14161 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt tcggggtatc
    14221 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
    14281 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
    14341 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
    14401 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
    14461 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
    14521 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtta taaagatgtc
    14581 ctgaacgatg accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcatc
    14641 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
    14701 gtgcggcagc tcgctgatgc gatagccggt atgagcaggc aggctttcca cgccccaggc
    14761 tttggcgaat ttttcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
    14821 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
    14881 acccgcatgc ggcttaccga ggttactggt cagcatcgcg aggctggtca gagaacgcac
    14941 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
    15001 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacac cggtgatatc
    15061 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
    15121 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
    15181 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
    15241 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
    15301 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
    15361 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
    15421 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
    15481 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
    15541 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
    15601 ggcaacgtaa ttcagtgcct catctcagga aacaggttcg agtttgccgc cacgctggcg
    15661 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
    15721 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
    15781 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
    15841 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
    15901 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
    15961 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
    16021 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
    16081 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
    16141 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
    16201 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
    16261 cgcgttgctt ccacgtgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
    16321 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
    16381 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
    16441 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
    16501 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg atagaacaac
    16561 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
    16621 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
    16681 ggtaatgcca caggtttgat ctccggcata tcgctggctc ccgcgccaat caatgcgcgc
    16741 agagattctc tcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
    16801 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
    16861 ctttggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
    16921 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
    16981 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
    17041 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
    17101 gtatagtacg gcccgtccat ccccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
    17161 ccgttcgccg agacacgttg gcggttaagc atccctaacg ctgccacctg caactgtgaa
    17221 ccggcatcta acaaatcggc ctgcgattgc gctttttctt cccgcagttt cgcttcggcg
    17281 agggtgtgtg aaccacttag cgtccgttgg atcagggcat ccaactgcgg gtcattgagt
    17341 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc atcggccagt
    17401 ttgatttgtt ccggtttgag ctgttgatga ggcgcagaat ccttacgtac cagggcacag
    17461 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
    17521 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
    17581 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
    17641 ggatggcctg cgaggtgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
    17701 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgacctgct ctgtccggct
    17761 gtagtgcgtt atccagcgcc tgcgccgaat tccaggtggc atcccaggcg tgaaggatat
    17821 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
    17881 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
    17941 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
    18001 tccgcattac tttacttaac atacccagcg cgccagccag tttttgcggc agcgtgcgcg
    18061 cttcactttc aggccagaca aaggtgtaaa tcatcgcgga caccacggta ccaatgatga
    18121 tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga
    18181 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
    18241 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
    18301 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
    18361 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt ggattagcga
    18421 cgatcacgca ggtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg
    18481 tgtaacagat caaacaggcg agcaatgttt ttaccgcgta gcgtatatag tctggattgg
    18541 taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt
    18601 tcatctggcc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
    18661 ccaccgcttc actttcactg agccgccagt cgctttgcca gcactgacct tcagcaacgg
    18721 catgctgcag cttgttgatt tctgaggcta atttttgccg aaattcaata attgcctgag
    18781 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
    18841 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
    18901 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
    18961 cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcttgatgca
    19021 tttgcgtaat ggcacgattg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
    19081 gatagaggcc aacaacgata caccacagcg ttaagcgcac gactacttcc ggatagtcga
    19141 gcatggcggg gaaggtttgc ccgtaaatgg cgacagcgaa aaagaccagt cccagccgat
    19201 gggtgcgcat caaaaacatg cagcccatca ggatcggccc ggcgatgatc aatcggatca
    19261 acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg
    19321 caaccacaaa caagatcgcc acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa
    19381 acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca
    19441 ccagcaggca gcctacccag agttgcacga tctgcggcac gcggccgggt cgccgctcgc
    19501 ttaactcttc atgaaagaac gccagcagcc tgaccaccgg taatggcagg gagttgagcg
    19561 cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg
    19621 gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat
    19681 agaacgagac acttttggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc
    19741 gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca tcaggcgaat
    19801 cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac
    19861 gtaccagtga cgagtgtcga tgagggtaaa aatagggcgc atggcagaag caaattgccc
    19921 gacggaggtt ttgagggaaa tgatccggcc atcaaatggc gcgcgaacgg tcgtcatttc
    19981 cagatgtagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc
    20041 atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg cattaagatc
    20101 cgcttctgcg gcgcgctgcg ccgttcttgc acggtcaacg tcttctgctg agacaaaacc
    20161 ctctttcagt aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttcg cggcggcacg
    20221 ggctttttct accgtggcat taaccgagtc ggcaccaaac tgttgggcgt caacgctacg
    20281 ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcggctt tcgccaggtt
    20341 ggcttcgtac gggcgcgggt cgatgcggaa cagcaaatcg ccctgtttca ccgcctggtt
    20401 gtcggtgacc gccagttcta caatgcgacc gctgacttcc ggcaccacat caatggtatc
    20461 tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac
    20521 aagggcaacc agcgccaacg caaccactaa cagagcaggg aatttactgc gaggtgcttt
    20581 tttcggcgta ctttccatga gcgcgtctca aatagattag aaaaatgcca gccagaacag
    20641 catggcgtag agagcaaaaa gggcggtata aataattccg acaaatgcca gattgatatt
    20701 tgcccg
//
LOCUS       DAZPFP010000051        20691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPFP010000051 DAZPFP010000000
VERSION     DAZPFP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.051"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHUR4_001869"
     CDS             81..350
                     /locus_tag="ACHUR4_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1455473.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHUR4_001870"
     CDS             344..709
                     /locus_tag="ACHUR4_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1455474.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUR4_001871"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUR4_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1455475.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUR4_001872"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUR4_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1455476.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUR4_001873"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUR4_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1455477.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUR4_001874"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUR4_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1455478.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUR4_001875"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUR4_001875"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1455479.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUR4_001876"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUR4_001876"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1455480.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHUR4_001877"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHUR4_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUR4_001878"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUR4_001878"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1455481.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUR4_001879"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUR4_001879"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1455482.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUR4_001880"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUR4_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR1455483.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHUR4_001881"
     CDS             complement(16429..17187)
                     /locus_tag="ACHUR4_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1455484.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUR4_001882"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUR4_001882"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1455485.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUR4_001883"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUR4_001883"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1455486.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUR4_001884"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUR4_001884"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1455487.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHUR4_001885"
     CDS             complement(20306..20587)
                     /locus_tag="ACHUR4_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455488.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4888 a         5692 c         5217 g         4894 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg ccctgatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZPFP010000052        20597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPFP010000052 DAZPFP010000000
VERSION     DAZPFP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.052"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUR4_001886"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUR4_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1455489.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUR4_001887"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUR4_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1455490.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUR4_001888"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUR4_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1455491.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUR4_001889"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUR4_001889"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1455492.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUR4_001890"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUR4_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1455493.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHUR4_001891"
     CDS             complement(8217..9110)
                     /locus_tag="ACHUR4_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1455494.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUR4_001892"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUR4_001892"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1455495.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUR4_001893"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUR4_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1455496.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUR4_001894"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUR4_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1455497.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUR4_001895"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUR4_001895"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1455498.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUR4_001896"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUR4_001896"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1455499.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHUR4_001897"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHUR4_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUR4_001898"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUR4_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1455500.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUR4_001899"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUR4_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1455501.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUR4_001900"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUR4_001900"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1455502.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUR4_001901"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUR4_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1455503.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4935 a         5702 c         5016 g         4944 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaa
//
LOCUS       DAZPFP010000053        20523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPFP010000053 DAZPFP010000000
VERSION     DAZPFP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.053"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHUR4_001902"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHUR4_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1455504.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHUR4_001903"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHUR4_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR1455505.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHUR4_001904"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHUR4_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1455506.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUR4_001905"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUR4_001905"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1455507.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUR4_001906"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUR4_001906"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1455508.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACHUR4_001907"
     CDS             4451..4816
                     /locus_tag="ACHUR4_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1455509.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACHUR4_001908"
     CDS             4966..5484
                     /locus_tag="ACHUR4_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1455510.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUR4_001909"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUR4_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACHUR4_001910"
     CDS             6715..7197
                     /locus_tag="ACHUR4_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1455511.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUR4_001911"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUR4_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1455512.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUR4_001912"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUR4_001912"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1455513.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUR4_001913"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUR4_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1455514.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUR4_001914"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUR4_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1455515.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUR4_001915"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUR4_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1455516.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACHUR4_001916"
     CDS             10941..11732
                     /locus_tag="ACHUR4_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1455517.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACHUR4_001917"
     CDS             11753..13039
                     /locus_tag="ACHUR4_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1455518.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUR4_001918"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUR4_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1455519.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUR4_001919"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUR4_001919"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1455520.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACHUR4_001920"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACHUR4_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1455521.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUR4_001921"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUR4_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1455522.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACHUR4_001922"
     CDS             complement(16987..17277)
                     /locus_tag="ACHUR4_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1455523.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUR4_001923"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUR4_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1455524.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUR4_001924"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUR4_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1455525.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUR4_001925"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUR4_001925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACHUR4_001926"
     CDS             19139..20380
                     /locus_tag="ACHUR4_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1455526.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAZPFP010000054        20186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPFP010000054 DAZPFP010000000
VERSION     DAZPFP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.054"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHUR4_001927"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHUR4_001927"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1455527.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUR4_001928"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUR4_001928"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1455528.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUR4_001929"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUR4_001929"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1455529.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUR4_001930"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUR4_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1455530.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUR4_001931"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUR4_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1455531.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUR4_001932"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUR4_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1455532.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUR4_001933"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUR4_001933"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1455533.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHUR4_001934"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHUR4_001934"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1455534.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUR4_001935"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUR4_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1455535.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUR4_001936"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUR4_001936"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1455536.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUR4_001937"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUR4_001937"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1455537.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHUR4_001938"
     CDS             8212..9075
                     /locus_tag="ACHUR4_001938"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1455538.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUR4_001939"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUR4_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1455539.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHUR4_001940"
     CDS             10411..11028
                     /locus_tag="ACHUR4_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1455540.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUR4_001941"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUR4_001941"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1455541.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUR4_001942"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUR4_001942"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1455542.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUR4_001943"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUR4_001943"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1455543.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUR4_001944"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUR4_001944"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1455544.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUR4_001945"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUR4_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1455545.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHUR4_001946"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHUR4_001946"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1455546.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUR4_001947"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUR4_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1455547.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
BASE COUNT         4947 a         5499 c         5099 g         4641 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgt
//
LOCUS       DAZPFP010000055        20169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPFP010000055 DAZPFP010000000
VERSION     DAZPFP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.055"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUR4_001948"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUR4_001948"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1455548.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUR4_001949"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUR4_001949"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1455549.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHUR4_001950"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHUR4_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHUR4_001951"
     CDS             complement(3183..4388)
                     /locus_tag="ACHUR4_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455550.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHUR4_001952"
     CDS             complement(4488..4844)
                     /locus_tag="ACHUR4_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1455551.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHUR4_001953"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUR4_001953"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUR4_001954"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUR4_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1455552.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHUR4_001955"
     CDS             6774..9830
                     /locus_tag="ACHUR4_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1455553.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHUR4_001956"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHUR4_001956"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1455554.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHUR4_001957"
     CDS             10337..10525
                     /locus_tag="ACHUR4_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1455555.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUR4_001958"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUR4_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1455556.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUR4_001959"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUR4_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1455557.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUR4_001960"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUR4_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1455558.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUR4_001961"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUR4_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1455559.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUR4_001962"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUR4_001962"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1455560.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUR4_001963"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUR4_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1455561.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUR4_001964"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUR4_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1455562.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUR4_001965"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUR4_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1455563.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHUR4_001966"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHUR4_001966"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1455564.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19768..19962)
                     /locus_tag="ACHUR4_001967"
     CDS             complement(19768..19962)
                     /locus_tag="ACHUR4_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455565.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5283 a         4849 c         5157 g         4880 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtg
//
LOCUS       DAZPFP010000056        20144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPFP010000056 DAZPFP010000000
VERSION     DAZPFP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.056"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(49..261)
                     /gene="cspA"
                     /locus_tag="ACHUR4_001968"
     CDS             complement(49..261)
                     /gene="cspA"
                     /locus_tag="ACHUR4_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1455566.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(542..832)
                     /locus_tag="ACHUR4_001969"
                     /pseudo
     CDS             complement(542..832)
                     /locus_tag="ACHUR4_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1266..1976
                     /locus_tag="ACHUR4_001970"
     CDS             1266..1976
                     /locus_tag="ACHUR4_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1455567.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2026..3000)
                     /gene="ghrB"
                     /locus_tag="ACHUR4_001971"
     CDS             complement(2026..3000)
                     /gene="ghrB"
                     /locus_tag="ACHUR4_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1455568.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3104..3764)
                     /locus_tag="ACHUR4_001972"
                     /pseudo
     CDS             complement(3104..3764)
                     /locus_tag="ACHUR4_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            3917..6115
                     /locus_tag="ACHUR4_001973"
     CDS             3917..6115
                     /locus_tag="ACHUR4_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR1455569.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6084..6524)
                     /locus_tag="ACHUR4_001974"
     CDS             complement(6084..6524)
                     /locus_tag="ACHUR4_001974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1455570.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6521..7084)
                     /gene="tag"
                     /locus_tag="ACHUR4_001975"
     CDS             complement(6521..7084)
                     /gene="tag"
                     /locus_tag="ACHUR4_001975"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1455571.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7242..7940
                     /locus_tag="ACHUR4_001976"
     CDS             7242..7940
                     /locus_tag="ACHUR4_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1455572.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8169..9377
                     /locus_tag="ACHUR4_001977"
     CDS             8169..9377
                     /locus_tag="ACHUR4_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455573.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9701..11392
                     /gene="eptB"
                     /locus_tag="ACHUR4_001978"
     CDS             9701..11392
                     /gene="eptB"
                     /locus_tag="ACHUR4_001978"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1455574.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11484..11560
                     /locus_tag="ACHUR4_001979"
     tRNA            11484..11560
                     /locus_tag="ACHUR4_001979"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11518..11520,aa:Pro,seq:cgg)
     gene            12304..13911
                     /gene="dppA"
                     /locus_tag="ACHUR4_001980"
     CDS             12304..13911
                     /gene="dppA"
                     /locus_tag="ACHUR4_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1455575.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14219..15238
                     /gene="dppB"
                     /locus_tag="ACHUR4_001981"
     CDS             14219..15238
                     /gene="dppB"
                     /locus_tag="ACHUR4_001981"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1455576.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15248..16150
                     /gene="dppC"
                     /locus_tag="ACHUR4_001982"
     CDS             15248..16150
                     /gene="dppC"
                     /locus_tag="ACHUR4_001982"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1455577.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16161..17144
                     /gene="dppD"
                     /locus_tag="ACHUR4_001983"
     CDS             16161..17144
                     /gene="dppD"
                     /locus_tag="ACHUR4_001983"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1455578.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17141..18145
                     /gene="dppF"
                     /locus_tag="ACHUR4_001984"
     CDS             17141..18145
                     /gene="dppF"
                     /locus_tag="ACHUR4_001984"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1455579.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18175..19446)
                     /locus_tag="ACHUR4_001985"
     CDS             complement(18175..19446)
                     /locus_tag="ACHUR4_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1455580.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            19922..20029
                     /locus_tag="ACHUR4_001986"
     CDS             19922..20029
                     /locus_tag="ACHUR4_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1455581.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4802 a         5008 c         5334 g         5000 t
ORIGIN      
        1 tccccgccaa atggcaggga tcttagattc tgtgctttta agcagagatt acaggctggt
       61 tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca ctttctgacc
      121 ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga acacatcttt
      181 agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc attttacgat
      241 accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc ctttcggcga
      301 tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag gggtatcaac
      361 gataactctt gaagggactt gccttactac actgctttaa tggtctgtac gtcaaaccgt
      421 tgatgtgcat taagccactc attggcgggt gatgcaacaa ttatttttca tatttatgat
      481 taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag tggataaaag
      541 tctatttcat caactgctta cttaatgccg ggttggcttg aatcaaacgc atcaatttta
      601 gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg gcgactgata
      661 cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt cccttttcat aactgctcaa
      721 tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca tctttaaaaa
      781 caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc attgaaaact
      841 cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat agtcgggaaa
      901 agcagacaca gcggggtgaa gtcagggcat gctctggcta tgattcgatt cagcctgtca
      961 tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct cttatcactc
     1021 ctgttttgta aagatttcgc aaatctaacc ttctgcgaag gcgtaaatct ctggctacaa
     1081 aaaaggcaac ggcactatca ttttttaaca atgaaacaac aaattaacct tgtttcattt
     1141 tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc tgtcctggac
     1201 tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt gaaaggagta
     1261 agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg gcgttattaa
     1321 tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa gcgtttatcg
     1381 acttcctgca aaatacagtg atgcgtagcg gtgaacgtct accaaccctg actgccgatc
     1441 agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt tattctcagc
     1501 aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt aacgcaattc
     1561 gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaat ggttcgctgg
     1621 gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg cactctgcgc
     1681 taaaacagag cgatgacctg aaaccggtat ttgatcaggc attcaccaaa gtggtaacga
     1741 cgccagctga tgcattgcaa ccgttgattc cggcggcaca aactttcacg caacaactgg
     1801 tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc gcaaatggca
     1861 tccagttccc gacttcacag caggcaagtg aatataacaa actgattgcg ccattaccgg
     1921 cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc caataaagag
     1981 taaaagcccg agcgattatc gggctttact tcacgcagtc gcagcttagt ccgcgacgtg
     2041 aggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat tatccacggc
     2101 acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg gtactgcgac
     2161 gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt cgaagacatc
     2221 cagcccggcg gcgtgaattt cgcctttctg caatgctgcg atcagtgcat tttcgtcaac
     2281 caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg cgaattgttc
     2341 tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa cgaaatctga
     2401 ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt ctgcttcttt
     2461 atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgca cacgttgtgc
     2521 cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt gaacgtcagt
     2581 gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttaccc gttctgctac
     2641 ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg cgacggtttc
     2701 tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg catcgacatc
     2761 aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca ttttttccag
     2821 caatgcggca tctacattct cgtttgaacc cagtaaacct tcagcttctg caaaaattgc
     2881 tgcattttga acgacggttt gtgggctgag gtttgccacc tggtgaacgg tgaaatgcgc
     2941 ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg acggcttcat
     3001 gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg aaggaaaact
     3061 gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc gggcttaagg
     3121 taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg gcgattgggt
     3181 tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg gtgatcaacg
     3241 cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg tgaccccccg
     3301 tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc agcaccattg
     3361 ccacgccggt cagggtgtta gcgcctgccg gtttcagggg agcgctgctg ctgtcgaagg
     3421 tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg ctaacaccag
     3481 tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa cccacgccgc
     3541 cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga tctttcttcg
     3601 aagaagagag cgcaccaata cccgcgccca cgagagagcc cagacctgcg ccgatagcag
     3661 atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat accgccagag
     3721 cgccactcac tacggcggca agtaagataa acacgtttct tcattgttaa tccttaataa
     3781 cctttttatt ctttgtcacg ggttccgtgg cgggagatta tgccgcgtga acatgaagat
     3841 tattcctggg aatactcgga aatttgtaag taatatttaa ctgctcaata catctaacct
     3901 ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat tccgttctga ctgccgccca
     3961 ctgggggccc atgctggttg aaaccgacgg cgaaaccgtg tttagctcgc gtggcgcgtt
     4021 agccacagga atggaaaact ccttgcagag cgcggttcgc gaccaggttc acagcaatac
     4081 gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg tcaccggaaa acccgcaagg
     4141 cattcgtggg caggatgaat ttgttcgcgt gagttgggat gaggcgctgg caggcggtta
     4201 taccgggcat ctgggggatt attcgaccgg tgcggcacag gcgatcatgc cgtatgtcgt
     4261 aggtggcagc gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga
     4321 tgtcgtggtg ttgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc
     4381 cgatgagcag gggctttctt acttttccgc actgcgtgac agtgggaaaa agctgatctg
     4441 cattgatcca atgcgatcgg aaaccgtcga tttctttggc gataaaatgg agtgggtggc
     4501 accgcacatg ggcaccgatg ttgcgctgat gctggggatc gcccatacgc tggtggaaaa
     4561 tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc
     4621 ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg cagagatttg
     4681 tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa ataccaccat
     4741 gctgatggca ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgat
     4801 cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc
     4861 ttaccatttt gccaatggtg gtgaccccac gcggcgttct gcggtgctct cttccatgca
     4921 gggcagcttg ccgggtggct gcgatgcggt ggataaaatc cctgttgccc gcattgttga
     4981 agcactggaa aaccctggtg gcgcatatca acacaacggt atgaaccgac atttcccgga
     5041 tattcgtttt atctggtggg cgggcggtgc caactttact catcatcagg ataccaatcg
     5101 tctgatccgt gcctggcaaa aaccggagct ggtggtgatc tctgaatgct tctggacggc
     5161 ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc tcttttgagc gtaatgatct
     5221 caccatgacc ggtgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc
     5281 acgctatgaa gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac gttgggagaa
     5341 gggcggctat gcgcggttta cggaaggaaa aagtcagctg caatggctgg aaacgtttta
     5401 taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt
     5461 ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg
     5521 cttcgctgat ttttgccgcg atccgctggc gcatccgtta aaaaccgcca gcggcaagat
     5581 tgaaatcttc tcacagcgta ttgccgatta cggttacccg gattgccctg ggcatccaat
     5641 gtggctggag ccggacgaat ggcagggcaa tgccgaacca gaacagttgc aggtactttc
     5701 tgcccatccg gcgcaccgcc tgcacagcca gctgaattac agttctctgc gcgaattgta
     5761 cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat
     5821 aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg cagatccttg ccggagcggt
     5881 cattagcgag ggaattaaac ctggcgtgat ttgcattcac gaaggggcat ggccggatct
     5941 ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct
     6001 ccccagctcg cggctgggga atggctgtgc gggtaatacg acgctggcat ggctggaaaa
     6061 atacaacggt ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag
     6121 gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg
     6181 taaaaatcta tcgccggttg atttttttga taaacctcca gcatcagatg gggatagcgc
     6241 tgctgcacat actgcatcag cgccttacca ataccgcgtc tgacggcctt cggtgcgaca
     6301 aacattgctg ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtcc
     6361 tcttcccaga cccagttttg cgcgttggca agataggcat cccgcaccag cggaatgcag
     6421 tcacgccagt aattcgcttt tataaaggga tgcccccagg ttgtactttc cagccacagt
     6481 tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa tcatggttta tttcccagat
     6541 agcaacagca gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac
     6601 agattgtggt gccgacaaac ttaaaaccac gttttttcag tgccttagat agggcgtcgg
     6661 aggcggacgt agatgtggga atttcgctca acgttgtggc ttgtgtcacc tgtggctgat
     6721 gatttacaaa cgaccagaca aagtcgggaa acggttcgcc gttctgttcc atttgcaggt
     6781 acgcccgcgc attaccaata attgcctgaa ttttccctcg atggcggata atcccggcgt
     6841 cctgtaccag tctttcgaca tcctcttcct gcattgctgc gaccttcacc ggatcgaact
     6901 gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataacccag
     6961 cctgctgccc ttcaaagcag atcatttcga acagtttttt actgtcagtt tcaggcacgc
     7021 cccactcatt atcatggtag gcaatataaa gcgggccctg actcacccag ccgcaacgtt
     7081 ccatactttc cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat
     7141 cggtgaaaaa gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac
     7201 aataatattg ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg
     7261 gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat cagtgccttt tttctcaaca
     7321 cagcttacgc ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc
     7381 gttacacctg ggatagtgat catcaacctg attacaacga tattttgtcg caacgtattc
     7441 aaagtagcca aagggcgctg ggactggaag tcaatctggc ggaagaaact cctgtggatg
     7501 tgaccagcag tatgagtatg ggctggaatt ttcctttata tgaacaggtt acaaccggcc
     7561 cggtcgcggc attacattac gatggcacaa ccacctcgat gtataacgag tttggcgaca
     7621 gtactaccac gctgaccgat ccgttatggc atgccagcgt gagtagctta ggctggcgtg
     7681 ttgactcccg gcttggcgat ctccgaccct gggcgcaaat cagctataac cagcaatttg
     7741 gcgagaatat ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg
     7801 gcaactggct ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgccgcct
     7861 atgctgcgct aactcaggca gaaaatacca ctaataatag cgactatctg tatacgatgg
     7921 gggttagcgc cagattttaa cgtaacagtc acaattgaaa ccattgaata acaacagttg
     7981 tggcgatagt gggtgctaac ttaccaaata ataaatttgg tgaataattg tcgcgtcatt
     8041 cattcctgaa ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccaa
     8101 gcataaaatg cacttcgtta tggttgtcgg cagagatttt tcctttttat tactgcagga
     8161 atactgccat gacaccttca aattatcagc gtacccgctg gctgacactc atcggtacta
     8221 tcattaccca gtttgcgctg gggtcggttt atacccggag cctgtttaat ggcgcgcttt
     8281 ctgccaagct ggatgcgccg gtaagccagg tcgctttctc tttcggcttg ttaagtctgg
     8341 ggctggcaat ttcgtcttct gttgcgggca aattgcagga acgttttggc gttaaacgcg
     8401 tcaccgtggc ttccggcatt ttgctgggat taggcttctt cctgaccgcg cattccaaca
     8461 acctgatgat gctgtggtta agcgccggtg tgctggtcgg tctggcggat ggcgcgggtt
     8521 acctgctgac gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt ctgatctccg
     8581 cgttcgctat cggttcttat ggtctgggta gcctgggttt caaatttatc gacacgcagc
     8641 tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga
     8701 tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca
     8761 atggtgtggt ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg cagtactgga
     8821 tgttagcggt aatgttcctg actgcctgca tgagcggcct gtacgtgatt ggggtagcga
     8881 aagatatcgc ccaaagtctg gcacaccttg atgtggtttc cgcagccaat gcagtaacgg
     8941 ttatttccat cgctaacctt tcaggtcgtc tggtgctggg cattctgtct gacaaaattg
     9001 cccgtatccg tgttatcacc attggtcagg tgatttcgct ggtgggtatg gcggccctgc
     9061 tgtttgcacc attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact
     9121 ttggcggcac tattaccgtc tttccatcac tggtcagtga gttcttcggc ctcaataacc
     9181 tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat cggtagcatt tttggttcga
     9241 ttatcgcctc actgtttggc ggtttctatg tgaccttcta cgtgattttc gccctgctga
     9301 ttctgtcatt ggcgctttct acgacgattc gtcagccaga gcagaaaatg ttgcgtgagg
     9361 cgcatggctc cctttaatgc tcagccgcga gctactgggc ttcagcttcc gtgtgcatca
     9421 aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt tgggccagtt aactggccct
     9481 ttttactgct ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg
     9541 cttcatgcac actctttccc cacacttttt cgctttgctg tggtctactt attcgcgcgt
     9601 gtagatttta cttatctgac tacctccgca cttttcccct gccgggcctg aaaagccgct
     9661 aagcagggtg ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat
     9721 tacacagcag aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc
     9781 ggttttttat cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcgtttc
     9841 tgctgttgtt gaactggccg ccaccgtact ggtgaccttc tttttactac gtcttctatc
     9901 gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg gtggtgctct tttccgcagg
     9961 tgccagctat tacatgacct tccttaatgt ggtcattggt tatggcatca tcgcttccgt
    10021 catgaccacc gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt
    10081 aatcgccgtt agtgcattgc ctcttatcct tatctggaat aaccgctgtc gctacacctt
    10141 gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc ctggtggtcg tcgtactggc
    10201 gggtattatg gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag
    10261 ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta gcgaactctt atctgccatc
    10321 aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg gtcgatgaat cttccgataa
    10381 taattcattg cttaatccgg cgaagaagtt tacataccag gctccacaaa acgttgatga
    10441 tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg gaccatatgg gtattttcgg
    10501 ctatgagcgt aataccacgc cgaaactggc ccaggagaaa aatctggcgg cgttccgtgg
    10561 ttactcctgt gataccgcaa ccaaactctc actgcgttgc atgtttgtac gtcagggggg
    10621 cgcggaagat aatccgcagc gcacattaaa agaacagaac attttcgcgg ttctgaagca
    10681 gttaggattc agttctgacc tctacgctat gcagagcgaa atgtggttct acagcaacac
    10741 gatggcagac aacattgctt atcgtgagca gattggtgcg gagccacgta atcgtggcaa
    10801 gccggtagat gatatgttgc tggtagacga aatgcagcaa tcgctagggc gcaacccgga
    10861 tggtaagcat ctgatcattc tgcataccaa aggttcgcat tttaactaca cccagcgtta
    10921 tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa
    10981 agcgcagatg atcaactcct atgacaactc ggtgacctat gtggatcact ttatctccag
    11041 cgtgattgat caggttcgcg ataagaaagc gattgtgttc tacgcagctg accacggtga
    11101 gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggagca
    11161 gttccgcgta ccgatgatgg tctggatgtc agataaatat ctggaaaatc cggccaatgc
    11221 gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc gtcacgtaga
    11281 gctgtacgac accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga
    11341 aaacaacaac tggtgtcaca tcccgcagac aaaagtggca gcggctaact aagcggcctg
    11401 ctgactttct cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg
    11461 cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg
    11521 gacgaagggg tcggaggttc gaatcctcta tcaccgacca aattcgaaaa gcctgctcaa
    11581 cgagcaggtt tttttgcatt tgtagctcat gaggatgaga acctccgggg gcagggaggc
    11641 tcgactcgac gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact
    11701 tattgtcgga tgcggcgcga ccgccttatt cgaccaacac accacacatt ttcctgccaa
    11761 ctttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt
    11821 ttatatttgc ttaaatcttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg
    11881 cgttttagcg ttcatggtat taatcgctca gatatccacc ttttcctcag atttttttca
    11941 ttaaatgcat aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggtta
    12001 tgttggagta gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg
    12061 tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct
    12121 gttgacagat tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg
    12181 ttaattagta cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag
    12241 gtcaggcagc gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga
    12301 ataatgcgta tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct
    12361 atgaccgtcg cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg
    12421 gaagggttta acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtcccg
    12481 ctttataacc gcctggttga atttaaaatc ggcaccaccg aagtgatccc gggcctcgct
    12541 gaaaagtggg aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg
    12601 aagtggcacg acaataaaga attcaaaccg acgcgcgaaa tgaacgccga tgacgtggtg
    12661 ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc tggcggcagc
    12721 tacgaatact tcgaaggtat gggcttgccg gagctgatca gcgaagtgaa aaaggtggac
    12781 gataacaccg ttcagtttgt gctgactcga ccggaagcgc cgttcctcgc tgacctggca
    12841 atggacttcg cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca
    12901 ccggaaaaac tggacctcaa cccaatcgga accggtccgt tccagttaca gcagtaccaa
    12961 aaagattccc gtatccgcta caaagcgttt gatggctact ggggcaccaa accgcagatc
    13021 gatacgctgg tcttctctat tacccctgat gcttccgtgc gttacgcgaa attgcagaag
    13081 aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca tcgctcgcat gaagcaggat
    13141 aaatccatca acctgatgga aatgccgggg ctgaacgtcg gctacctctc gtataacgtg
    13201 cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac taacctacgc ggtgaacaaa
    13261 gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg
    13321 ccaaccatgt ggggctataa cgacgacgtt caggattaca cctacgatcc tgaaaaagcg
    13381 aaagccttgc tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg
    13441 ccggtacaac gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac
    13501 tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg aatggggtga gtacctcaag
    13561 cgtgcgaaag atggcgaaca tcagacggta atgatgggct ggactggcga taacggggac
    13621 ccggataact tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ttctaactac
    13681 tcaaaatggt gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac
    13741 cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg
    13801 gcactgatca tcgctcactc caccgtgttt gaaccggtac gcaaagaagt caaaggctat
    13861 gtggttgatc cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc
    13921 atacaagact gatggcaaag gcaaaactgc ctgatgtgct ccgcttatca ggcctacgaa
    13981 aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg gataaggcgt taacgccgca
    14041 tccggcataa acaaagcgca ctttgtcaac aatctgtata cccggtggcg ctgtgcctct
    14101 gcgtgcagcg ccatccggca gcaatacttc attctctgtc cgacaagggc gggaattgat
    14161 ttgtgagcaa tacagacacg cagttccagg ctgcgagtca ctatagagaa tccgggttat
    14221 gttgcagttt attctccgac gtttgggact cgtcatcccc acgtttatcg gtattaccct
    14281 tctcacattt gcctttgtcc acatgatccc gggcgatccg gtgatgatca tggcaggcga
    14341 acgtgggatc tcccctgagc gtcacgtgca gctgctggct gaactcggct tagataaacc
    14401 gatgtggcag cagtatctcc attacatttg gggcgtaatg cacggcgatc taggcatttc
    14461 aatgaaaagc cgaattccag tatgggaaga gttcgtgccg cgctttcagg ccacgctgga
    14521 acttggcgtc tgcgcgatga tttttgctac ggcagtcggt attccggttg gtgtgctggc
    14581 tgcggttaaa cgcggttcca ttttcgatca cactgcagtt ggcctggcgc tgaccggcta
    14641 ctcgatgccg atcttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa
    14701 cctgacgccc gtctccggtc gcgtgagcga tatggtgttc ctcgatgact ccaatccgtt
    14761 aaccggtttt atgctaatcg acaccgccat ctggggtgaa gacggcaact ttatcgatgc
    14821 cgtcgcccat atgatcttgc ccgccattgt gctgggtact attccgctag cggtcattgt
    14881 gcgtatgaca cgctcctcga tgctggaagt gctgggcgag gattacatcc gcaccgcgcg
    14941 cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct
    15001 gccggtagtg accgttatcg gcctccaggt gggaacattg ctggcggggg cgattctgac
    15061 cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg
    15121 cgactatccg gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa
    15181 cttgctggtc gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg
    15241 ggacatcatg tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc
    15301 gttacaggag ttctggcact attttaaacg caacaaaggc gcggtcgtcg ggctggttta
    15361 cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg attgcgcctt ataacccggc
    15421 ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg caggaaggcg gcagtatggc
    15481 acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg tcgcggctga tgtacggcgc
    15541 gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg tcgctgatta tgggcgttat
    15601 tctcggcctg atcgccggtt acttcggcgg cctggttgat aacattatca tgcgcgtggt
    15661 cgatatcatg ctggcgctgc caagtctgct gttggcactg gtgctggtgg cgattttcgg
    15721 cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt gccttgccgc actatgtgcg
    15781 cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg cctctcgcgt
    15841 ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc ttcccgaact gccttgcgcc
    15901 gctgattgtt caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg
    15961 tttcctcggc atgggggcac agccgccaac gcctgagtgg ggcaccatgc tctccgacgt
    16021 gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc cctggtctgg cgatcctgtt
    16081 gacggtgctg gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact
    16141 aaagcagtaa gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg
    16201 gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggcg
    16261 aagtggtcgg gattgtgggt gagtccggct ccggtaagtc ggtcagttca ctggcgatta
    16321 tggggctgat tgattatccg ggccgcgtaa tggcagaaaa actggagttt aacggccagg
    16381 atttgcagcg tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga
    16441 tcttccagga cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg
    16501 aagcgattaa agtgcatcag ggcggcaaca aaagtacccg ccgtcagcgg gcgattgacc
    16561 tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac ccgcatcagc
    16621 tttccggcgg catgagccag cgcgtgatga tcgccatggc gattgcctgt cggccaaaac
    16681 tgctgattgc cgatgaaccg accaccgcgc tggacgtgac cattcaggcg caaatcatcg
    16741 aactactgct ggagctacag cagaaagaga acatggcgct ggtgttaatt acccatgacc
    16801 tggcgctggt ggcggaagct gcgcataaaa tcattgtgat gtatgcaggt caggtagtag
    16861 aaacgggcga tgcacacgcc atcttccatg cgccgcgtca cccgtatact caggcattgc
    16921 tgcgtgcgct gccagaattt gctcaggaca aagaacgtct ggcgtcgttg ccaggtgttg
    16981 ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca
    17041 ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct cgctgatggg cgtcagtcca
    17101 aatgccatta cccacttgat gatgccggga ggcccacact atgagtacgc aagaggccac
    17161 ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg
    17221 catgttcgtg ccggaacgtc tggtgaaggc gctggacggc gtttcgttta accttgaacg
    17281 tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt aaatcgaccc tcggtcggtt
    17341 gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat tatcaggggc aggatctgct
    17401 caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa atccagatcg tcttccagaa
    17461 tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa attctcgaag agccgctgct
    17521 tattaacacc agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcaaa
    17581 agtcggcctg aaaaccgagc actatgaccg ctatccgcac atgttttccg gcggacaacg
    17641 tcagcgtatc gctattgccc gtggtctgat gctcgacccg gatgtggtga ttgccgatga
    17701 gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt
    17761 gcagcaggag ttggggcttt cttatgtctt tatctcccac gacctgtcgg ttgtggagca
    17821 cattgctgat gaagtgatgg tgatgtacct aggccgctgc gtggagaaag gaacgaagga
    17881 ccaaatcttc aataacccgc gccatccgta cacccaggcg ctgctctccg cgacgccacg
    17941 cctgaacccg gacgatcgtc gtgaacgcat caaactcacc ggtgaactgc caagcccgct
    18001 gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcac
    18061 ccagttgcag ccgcagctaa aagactacgg aggtcaactg gtagcttgtt ttgctgttga
    18121 tcaggatgaa aatccgcagc gttaactcac aaaaccgggg ccatgcgctc cggtttactc
    18181 aatcagcttc aacagtggcg acaagcatag caaaatgccg taaagcagaa tcagccaggc
    18241 tttaaatccg cgaagttttt ccagttgtgc gactttatag atcaggaaaa acggaatgag
    18301 gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagca ctgatacacg
    18361 aaacgaaacc caaatcgtca acgtaatgac gataaaagcg cagatcgcca gagtcagcat
    18421 gcgtgagtta attttcttgg tatcaataat tcggcttaac agattgagaa taatgccttt
    18481 aatagcctcg tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg caaagatatt
    18541 cagcaccgta gaggtgatat gaatgatatg tccagggatc acctgcgcgg ccagtgccag
    18601 tgccgagata ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa
    18661 cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata taactaattc ggtgggtacg
    18721 cagcgcgagc cgggttgcca gtactttatc tgcttcccgt ttacgatagg caatattcat
    18781 tgggttaagt acctgaataa ataccgcaga aaagaagcaa aatggaatgg taagcaggac
    18841 atcgcggaaa aagacggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg
    18901 gatcatcgca aaaccgaaca cgacaataat ccctactttg accaccacca ttggcccaga
    18961 aatcttaaac agtaatcgtt caccaccaga cgcaatcgct atcagtacgg cgaaaatagc
    19021 gactttataa agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta
    19081 cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa
    19141 aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac tgataatatc
    19201 ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgcactac
    19261 ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc
    19321 aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac
    19381 ccagccgaag tcgtagcgtg taaacggcaa tttctgattg tgtttcgata gtgtgttgtg
    19441 ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct
    19501 cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca
    19561 ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta ccagtctgga
    19621 gtcagcccgg atatgaactg caactattgc aaataaagaa attaagctgg aaggcatcgc
    19681 gcaatgagga agattgccgg gtgctgacag taaaaagcaa tcgtagaatc agcgtcaagc
    19741 cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc
    19801 tggagagaga aaacccccgc acgttgcagg tatgtacctg acaacataac gggggctaat
    19861 cttgaaccta gaccactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta
    19921 tatgacgctc gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc
    19981 tggcattctt gccagtatga tcgtgaactg gctgaacaag cggaagtagc gtgtcatgtg
    20041 ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag gccgcagggg tgaaactctg
    20101 cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taat
//
LOCUS       DAZPFP010000057        20031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPFP010000057 DAZPFP010000000
VERSION     DAZPFP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.057"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            252..1346
                     /gene="gcvT"
                     /locus_tag="ACHUR4_001987"
     CDS             252..1346
                     /gene="gcvT"
                     /locus_tag="ACHUR4_001987"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1455582.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHUR4_001988"
     CDS             1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHUR4_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1455583.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1878..4751
                     /gene="gcvP"
                     /locus_tag="ACHUR4_001989"
     CDS             1878..4751
                     /gene="gcvP"
                     /locus_tag="ACHUR4_001989"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1455584.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5018..5761
                     /locus_tag="ACHUR4_001990"
     CDS             5018..5761
                     /locus_tag="ACHUR4_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1455585.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5818..7251)
                     /gene="bglA"
                     /locus_tag="ACHUR4_001991"
     CDS             complement(5818..7251)
                     /gene="bglA"
                     /locus_tag="ACHUR4_001991"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1455586.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7296..7606
                     /gene="yqfB"
                     /locus_tag="ACHUR4_001992"
                     /pseudo
     CDS             7296..7606
                     /gene="yqfB"
                     /locus_tag="ACHUR4_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            7770..8429
                     /locus_tag="ACHUR4_001993"
     CDS             7770..8429
                     /locus_tag="ACHUR4_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1455587.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8625..9605)
                     /gene="ygfZ"
                     /locus_tag="ACHUR4_001994"
     CDS             complement(8625..9605)
                     /gene="ygfZ"
                     /locus_tag="ACHUR4_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1455588.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9848..10114
                     /gene="sdhE"
                     /locus_tag="ACHUR4_001995"
     CDS             9848..10114
                     /gene="sdhE"
                     /locus_tag="ACHUR4_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1455589.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10095..10502
                     /locus_tag="ACHUR4_001996"
     CDS             10095..10502
                     /locus_tag="ACHUR4_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1455590.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10542..11063)
                     /gene="fldB"
                     /locus_tag="ACHUR4_001997"
     CDS             complement(10542..11063)
                     /gene="fldB"
                     /locus_tag="ACHUR4_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1455591.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11175..12071
                     /gene="xerD"
                     /locus_tag="ACHUR4_001998"
     CDS             11175..12071
                     /gene="xerD"
                     /locus_tag="ACHUR4_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1455592.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12096..12806
                     /gene="dsbC"
                     /locus_tag="ACHUR4_001999"
     CDS             12096..12806
                     /gene="dsbC"
                     /locus_tag="ACHUR4_001999"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1455593.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            12812..14545
                     /gene="recJ"
                     /locus_tag="ACHUR4_002000"
     CDS             12812..14545
                     /gene="recJ"
                     /locus_tag="ACHUR4_002000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1455594.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14636..15734
                     /gene="prfB"
                     /locus_tag="ACHUR4_002001"
     CDS             join(14636..14710,14712..15734)
                     /gene="prfB"
                     /locus_tag="ACHUR4_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1455595.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15744..17261
                     /gene="lysS"
                     /locus_tag="ACHUR4_002002"
     CDS             15744..17261
                     /gene="lysS"
                     /locus_tag="ACHUR4_002002"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1455596.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17304..17852)
                     /gene="idi"
                     /locus_tag="ACHUR4_002003"
     CDS             complement(17304..17852)
                     /gene="idi"
                     /locus_tag="ACHUR4_002003"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1455597.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17907..17978
                     /locus_tag="ACHUR4_002004"
     CDS             17907..17978
                     /locus_tag="ACHUR4_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455598.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(17975..18100)
                     /gene="yqfG"
                     /locus_tag="ACHUR4_002005"
     CDS             complement(17975..18100)
                     /gene="yqfG"
                     /locus_tag="ACHUR4_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1455599.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18102..19550)
                     /gene="uacT"
                     /locus_tag="ACHUR4_002006"
     CDS             complement(18102..19550)
                     /gene="uacT"
                     /locus_tag="ACHUR4_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1455600.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4938 a         5005 c         5374 g         4714 t
ORIGIN      
        1 ctcatcacat catctttgta tagaaattgg tgtatttttt ggttttttat tctgtcgcga
       61 tttttgcatt ttttaaccat aagctaatgt gatgatcaat tttaccttat ggttaacagt
      121 ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg ttggggaccg ggagtggctc
      181 cgatgctggg ttttgtggtg ataatttcac catgaaaaag ttgtcagccc cgcttattca
      241 atgaggacaa gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc
      301 gcatggtgga tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac
      361 atcatgcggt acgtaccgat gccggaatgt ttgatgtgtc gcatatgacc atcgtcgatc
      421 tccgcggcag ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc
      481 tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag
      541 atgatctcat cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca
      601 cccgcgaaaa agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta
      661 ccgttcgtga tgacctttcc atgattgccg tgcaagggcc gaatgctcag gcaaaagctg
      721 ccacactgtt taatgatgct cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg
      781 tgcaggtggg cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa
      841 ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gcgggcgtta
      901 agccctgcgg tctgggggcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg
      961 gtcaggagat ggacgaaact atttctcctt tagccgccaa catgggctgg actatcgcct
     1021 gggaaccgac agatcgtgac tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg
     1081 gtacagaaaa actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc
     1141 cggtacgctt taccgatgcg cagggcaacc agcatgaagg cattatcacc agcggtactt
     1201 tctccccgac gctgggttac agcattgcgc tggcgcgcgt gccggaaggt attggcgaaa
     1261 cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa agtgacaaaa cctgtttttg
     1321 tgcgtaacgg caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt
     1381 accagcagaa ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac
     1441 ttacaccgtt ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga
     1501 cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcggt
     1561 aaaagcggcg tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc
     1621 actgagcgat tccccggaac tggtgaacag cgaaccgtat gcaggcggct ggatctttaa
     1681 aatcaaagcc agcgatgaaa gcgaactgga atcactgctg gatgcgaccg catacgaagc
     1741 attgttagaa gatgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga
     1801 aaatttatgc ctcaccctca ctctcttcgt aaggagagag gttcacaatt cactgcacgt
     1861 ttcaggaacc atcgctcatg acacagacgt taagccagct tgaaaacagc ggcgctttta
     1921 ttgaacgcca tatcggaccg gacgccgcgc aacagcaaga aatgctgaat gccgttggtg
     1981 cacaatcgtt aaacgcgctg accggccaga ttgtgccgaa agatattcag cttgcgacac
     2041 caccgcaggt tggcgcaccg gcgaccgaat acgccgcact ggcagaactc aaggctattg
     2101 ccagtcgcaa taaacgcttc acgtcttaca tcggcatggg ttacaccgcc gtgcagctac
     2161 cgccggttat cctgcgtaac atgctggaaa atccgggctg gtataccgcg tacactccgt
     2221 atcagcctga agtctcccag ggccgccttg aagcactgct caacttccag caggtaacgc
     2281 tggatttgac tggactggat atggcctctg cttctcttct ggacgaggcc accgctgccg
     2341 ccgaagcaat ggcgatggcg aaacgcgtca gcaaactgaa aaatgccaac cgcttcttcg
     2401 tggcttccaa tgtgcatccg caaacgctgg atgtggtccg tactcgtgct gaaacctttg
     2461 gttttgaagt gattgtcgat gacgcgcaaa aagtgctcga ccatcaggac gtcttcggcg
     2521 tgctgttaca gcaggtaggc actaccggtg aaattcacga ctacactgcg cttattagcg
     2581 aactgaaatc acgcaaaatt gtggtcagcg ttgccgccga tattatggcg ctggtgctgt
     2641 taactgcgcc gggtaaacag ggcgcggata ttgtttttgg ttcggcgcaa cgcttcggcg
     2701 tgccgatggg ctacggtggc ccacacgcgg cattctttgc ggcgaaagat gaatacaaac
     2761 gctcaatgcc gggccgtatt atcggtgtat cgaaagatgc agctggcaat accgcgctgc
     2821 gcatggcgat gcagactcgc gagcaacata ttcgtcgtga gaaagcgaac tccaacattt
     2881 gtacttccca ggtactgctg gcaaacatcg ccagcctgta tgccgtttat cacggcccgg
     2941 ttggcctgaa acgtatcgct aaccgcattc accgtctgac cgatattctg gcggcgggcc
     3001 tgcaacaaaa aggtctgaaa ctgcgccatg cgcactattt cgacaccttg tgtgtggaag
     3061 tggccgacaa agcgggcgta ctgacgcgtg ccgaagcggc tgaaatcaac ctgcgtagcg
     3121 atatcctgaa cgccgtgggg atcacccttg atgaaaccac cacgcgtgaa aacgtaatgc
     3181 agcttttcag tgtgctgctg ggcgataacc acgggctgga gatcgacacg ctggacaaag
     3241 acgtggctca cgacagccgc tctatccagc ctgcgatgct gcgcgacgac gaaatcctca
     3301 cccatccggt gtttaatcgc tatcacagcg aaaccgaaat gatgcgctat atgcactcgc
     3361 tggagcgtaa agatctggcg ctgaatcagg cgatgatccc gctgggttcc tgcaccatga
     3421 aactgaacgc cgccgccgag atgatcccaa tcacttggac ggaatttgcc gaactgcacc
     3481 cgttttgccc gccggaacag gctgaaggtt atcagcagat gattgcgcag ctggctgact
     3541 ggctggtgaa actgaccggt tacgacgccg tttgtatgca gccgaactcc ggcgcacagg
     3601 gcgaatacgc gggcctgctg gcgattcgtc attatcatga aagccgcaac gaagggcatc
     3661 gcgatatctg cctgatcccg gcttctgcgc acggaactaa ccccgcttct gcacatatgg
     3721 caggaatgca ggtggtggtt gtggcgtgtg ataaaaacgg caacatcgat ctgactgatc
     3781 tgcgcgcgaa agcggaacag gcgggcgata acctctcctg tatcatggtg acttatcctt
     3841 ctacccacgg cgtgtatgaa gaaacgatcc gtgaagtgtg tgaagtcgtg catcagttcg
     3901 gcggtcaggt ttaccttgat ggcgcgaaca tgaacgccca ggttggcatc acctcgccgg
     3961 gctttattgg cgcggacgtt tcgcacctca acctgcataa aactttctgc attccgcacg
     4021 gcggtggtgg tccgggtatg ggaccgatcg gtgtgaaagc gcatctggca ccgtttgtac
     4081 cgggtcatag cgtggtgcaa atcgaaggca tgttaacccg tcagggcgca gtttctgcgg
     4141 caccgttcgg tagcgcctct atcctgccaa tcagctggat gtacatccgc atgatgggcg
     4201 cagaagggct gaaaaaagca agccaggtgg caatcctcaa cgccaactat attgccagcc
     4261 gcctgcagga tgccttcccg gtgctgtata ccggtcgcga cggtcgcgtg gcgcacgaat
     4321 gtattctcga tattcgcccg ctgaaagaag aaaccggcat cagcgagctg gacattgcca
     4381 agcgcctgat cgactacggt ttccacgcgc cgacgatgtc gttcccggtg gcgggtacgc
     4441 tgatggttga accgactgaa tctgaaagca aagtggaact ggatcgcttt atcgacgcga
     4501 tgctggctat ccgcgcagaa attgaccagg tgaaagccgg tgtctggccg ctggaagata
     4561 acccgctggt gaacgcgccg cacattcaga gcgaactggt cgccgagtgg gcgcatccgt
     4621 acagccgtga agttgcggta ttcccggcag gcgtggcaga caaatactgg ccgacggtga
     4681 aacgtctgga tgatgtttac ggcgaccgta acctgttctg ctcctgcgta ccgattagcg
     4741 aataccagta attcactgat tcgagtattt tctaaaggcg cttcggcgcc tttttagtca
     4801 gatgacaaag tacaaaagtg ctcagacagt cccctcgccc ctttggggag agggttaggg
     4861 tgaggggaac aggccggcac tggcgcgaat atttaccctc accccgaccc tctccctgaa
     4921 agggcgaggg ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa
     4981 agatacgtca acgaattaat ttttctcgga aaaacaaatg gctatagcac ttgtgaccgg
     5041 tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgcaagaag ggtatacggt
     5101 ggcggttaat tatcagcaaa acctccacgc ggcgcaggaa gtgatgaact taataatgca
     5161 agccggtggc aaagcattcg tgctccaggc ggatatcagc gacgaaaacc aggtcgttgc
     5221 gatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg
     5281 gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact
     5341 ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct
     5401 taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg
     5461 ttcgccaggg gaatatgttg attacgcggc atcgaaaggg gcgattgatt cgttaaccac
     5521 cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt
     5581 tatttatacc gaaatgcacg ccagcggcgg cgagcctgga cgcgtcgatc gcgttaagtc
     5641 gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct
     5701 actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata
     5761 aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta
     5821 aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct tcttacgtga
     5881 acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt
     5941 gctgtactgc ccggtagtga atgacacgca gtcgatgcag ccccacggtg tgtagcccat
     6001 cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg cgcgcaggta
     6061 gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa
     6121 accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata
     6181 gcgcagacct actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg
     6241 tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt
     6301 ggtcatgtaa tagctgaaac caagataatc gcaggtgcct tcgcgcagta catcgagatc
     6361 gccgtcttcc attttgatat taaatccgcg acgttcccac tcgttcaata cataggacgg
     6421 gtaatagccg catagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc
     6481 gaacatcaca tcgtccgggt tacatgagta gggatagagc ggcaccatcg ccagcataca
     6541 gccgactttc atctccgggt taatgcgacg cgcggctttc actgccaggg cgctggcgac
     6601 aaactggtga tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtatacac
     6661 cacgccggag cagcagtaac caaacagcgg cgcacgccag ttacgctggt tgtttatttc
     6721 gttgaaggtc atccagtatt tgactttatg cttgtagcgt tcgaatacca cttccgcgaa
     6781 acggacaaag aaatcaacga atttacggtt ggtccagcta ccgtattgct gcaccagatg
     6841 cagcggcatt tcaaagtggg agagggttat caccggttcg atgttgtatt tcagcagttc
     6901 atcgaacaga gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt catcaccttt
     6961 tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa
     7021 tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta
     7081 ttttcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc cacaggtcag
     7141 aacgtcacaa atgctcggtc ctttgccgcc tttgttccag ccgccttcga cctgatgagc
     7201 ggcaacggcg ccaccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg
     7261 ctccagatca gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt
     7321 tcaacgtttc caggatgaca ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc
     7381 tgaatcgcac ttcaaaacgg gtgatgtgct tcgtgtcgga cgttttgaag atgacggtta
     7441 tttttgcacg attgaagtca ccgcaacctc aaccgtaacg ctggatacac tgacggaaaa
     7501 acatgcagag caggaaaata tgaccctgcc tgaactgaaa aaggtcattg ctgacatcta
     7561 tcccggtcag acacaatttt atatgattga atttaaatgt ctttaaattg aactcacgcc
     7621 tgttagttga aatttcgcat ttaatttcat gatttttagg agattagtct caaatcagaa
     7681 tttattttag ctaacaggtg ttcactggaa ctattctcag ttacgctgga gaggtataca
     7741 tgtgcgtgta cgtttctctg gagtaagtta tggttcagaa gcccctcatt aagcagggat
     7801 attcgctggc agaggaaatt gccaacagcg tcagtcacgg cattgggctg gtgtttggta
     7861 tcgttgggct ggtgttgcta ctggttcagg cggtggatct caatgccagc gccacagcga
     7921 taaccagtta cagcctctat ggcggcagta tgatcctgct gttcctcgct tcgacgctct
     7981 atcacgccat tcctcatcaa cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta
     8041 tttacctgtt gattgccgga acctacacgc cgtttttgct ggtggggctg gattctccgt
     8101 tagcgcgcgg gttgatgatt gttatctgga gcctagcatt gctggggatt ctgttcaaac
     8161 tgaccatcgc gcaccgattc aaaattttat ctctggtgac ttatctggcg atgggctggc
     8221 tgtcgctggt ggtaatttat gaaatggcag ttaagctcgc ggcgggcagc gttaccttac
     8281 tggcggtagg cggcgtggtt tattcgctcg gggtgatttt ctacgtctgc aaacgcattc
     8341 catacaacca tgccatctgg cacggcttcg tgctcggcgg tagtgtgtgc cactttctgg
     8401 cgatctattt gtatattggg caggcgtaat gggtatgcct ttgtaggcct gataagacgc
     8461 gtcagcgtcg catcaggcat cggagcgcaa ccgccggatg cggcgtgaac gccttatccg
     8521 atctacgggc ctcgtccctg ttgtgataaa ggcatcaaat cccagacatg ataagacgcg
     8581 tcagcgtcgc atcaggcgat aaatgcctga tgcgatacag agatttactc ttcgagcgaa
     8641 tacggcagcg gctcgatatg cagtgtattc gcatcgtcgc gtacgcggaa gatgctgtcc
     8701 ggttccatat cgttattcat gacgacctgt accacgacct gaccatcttc cagttttacc
     8761 gcagccagca ccgtaccggt acgacgccag ttctcgccca ttttcagctc taagtcttca
     8821 ccagcttccg gcagtcggct ggcactacct gccagcaacc agagcgcgcg tttgttggca
     8881 ccacggaatt ttgctcgcgc caccatctct tgtccggtat aacagccttt cttaaagctg
     8941 ataccgccca gcgcctggag attggtcgcc tgtgggataa actgcccgct gttggcggca
     9001 tcaatcaccg ggaaaccagc ttcaatgttt aatgccagcc actgttggct attgttcagt
     9061 tccgcttcac cgcgcagttt atcggtcagc atattagcag tagcttcatc ggttacgatc
     9121 aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc tttgactacc
     9181 tgtttttctt tcgaaggcag ttcgctaaag agatttgcca gcgcggcgcg cgcctgaaaa
     9241 ccggcaacac caagcagcac acgctcgtcg tctggcgcga tggtcacttt agagaatacc
     9301 gcatattttt tcagttcagt cagctgcggt tcacgcacgc tgcgccgttc aatccatgca
     9361 aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa
     9421 tgggcggcga gcaggtgctg atcttctgcc atctggctga catctgctgt cacctgaccc
     9481 tgcatatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc
     9541 atcagcgtca gcggtaaacg ggcagaagcc gtaggctgac gtggaggaaa aggtgtaaaa
     9601 gccataatag agtcctgagt tgcttaacgg cgtattaatg actaatggta aaagagctat
     9661 gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgcgaggcga ctgccaaaaa
     9721 aatgaagccc ttgaattgtt gtgaattaat ttcgcgatcg cgatggtgtt tactgatatt
     9781 cccgtaaaag aagtcgaaaa acacgttaca atagccgggt actggatttt cgtgagacag
     9841 gaagaagatg gacattaaca acaaagcccg aattcattgg gcatgccgcc gtggtatgcg
     9901 cgaactcgat atttcaatca tgtcgttttt cgaacatgag tacgacagct taagcgatga
     9961 cgaaaaacgc atctttattc gtctgctgga atgtgacgat ccggacctgt ttaactggct
    10021 gatgaatcac ggtaaaccgg ccgatgcaga actggaaata atggtccgac tcatccagac
    10081 acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt
    10141 ggctttcctt gctgattcat gggctggttg ccgctgttat tttactcatg ccctggccgc
    10201 tcagttacac cccgttatgg atggtgttac tttcgctggt ggtatttgat tgcgttcgca
    10261 gccagcggtg tattaatgct cgccaggggg aaattcgctt gttgatggac gggcgtttgc
    10321 gttggcaagg gcaggagtgg agcatcgtca aagcaccgtg gatgattaag agcggcatga
    10381 tgctgcgttt acgttctgat ggcggtaaac ggcaacattt atggctggca gccgacagca
    10441 tggacgaagc cgaatggcgg gatttacggc ggattttgtt gcaacaagag acgcaaagat
    10501 aaagatgacg gcaaagcata tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc
    10561 atttcgttga ggatttgctc gcaccagctc tgaatacgct cgtcgctaag gtcatactgg
    10621 ttagtttcat ccagcgccag acccacgaac agttgcccgt cagcaatcac cggtttcggg
    10681 ctggtaaatt catatccttc cgttggccag tagccgacga acttcacgcc tttggtcgag
    10741 agtttgtcat gcagcatacc gagcgcatcg aggaaccact cgccgtatcc cagttgatcg
    10801 ccaagcccat acagcgcaat aattttacct tcaaggttca ggtcgtcgag ctgatcccag
    10861 acggcttccc agtcttcctg gatttcacca aaatcccagg tcgggatacc cagaatgagc
    10921 acatcgtact gctccattaa tttcggggag tcgtccttga ggttatgtaa ggtcaccagt
    10981 tctgggccga taatatcgcg gattttttct gccgccattt cggtgtaaca ggtgctggaa
    11041 ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat tctgctgtgg cttttgcagc
    11101 tagtgtatca gaatcgcttt agtcacggca caataaggca taatggatca aatgagtgac
    11161 cataaggggc gcaagtgaaa caggagctgg cacgcatcga gcagtttctt gatgctctgt
    11221 ggctggaaaa aaatctggct gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga
    11281 tggtggagtg gttgcatcac cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc
    11341 aggcattact ggcagaacgg ctggagggcg ggtataaagc caccagctca gcgcgtttgc
    11401 tgagtgcagt gcgccgattg ttccagtatc tttatcgcga aaagtttcgt gaagacgatc
    11461 ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag
    11521 cgcaggtcga acgtttatta caggcgccat taattgatca gccactggag ctacgcgata
    11581 aagccatgct tgaagtgttg tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga
    11641 caatgagtga tatcagcctg cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag
    11701 agcgcctggt gccgttaggg gaagaggcgg tttactggct ggaaacctat ctggaacatg
    11761 ggcgtccgtg gctgctgaat ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc
    11821 agcagatgac gcgacagacc ttctggcacc gcattaaaca ttatgcagtg ctggcgggta
    11881 tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc ttttgccact catttattaa
    11941 atcatggtgc ggatttacgc gtggtgcaga tgctactggg ccacagcgat ctttccacca
    12001 cgcaaattta tacgcatgtc gctaccgagc gtctgcggca acttcatcaa cagcatcacc
    12061 cgcgggcgtg atgtctgaaa agaacgggaa gatttatgaa gaaaggtttt atgttgttta
    12121 ctttgttagc ggcgttttca ggctttgctc aggctgatga cgcggcaatt caacaaacgt
    12181 tagccaaaat gggcatcaaa agcagcgata ttcagcccgc gcccgtagct ggcatgaaga
    12241 cagttctgac taacagcggt gtgttgtaca tcaccgatga tggtaaacat atcattcagg
    12301 ggccaatgta tgacgttagt ggcacggctc cggtcaatgt caccaataag atgctgttaa
    12361 agcagttgaa tgcgcttgaa aaagagatga tcgtttataa agcgccgcag gaaaaacacg
    12421 tcatcaccgt gtttactgat attacctgtg gttactgcca caaactgcat gagcaaatgg
    12481 cagactataa cgcgctgggg atcaccgtgc gttatcttgc tttcccgcgc caggggctgg
    12541 acagcgatgc agagaaagaa atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt
    12601 ttgatgatgt gatggcaggt aaaagcgtcg caccagccag ctgcgacgtg gatattgccg
    12661 accattacgc acttggcgtc cagcttggcg ttagcggtac tccggcagtt gtgctgagca
    12721 atggcacact tgttccgggt taccagccgc cgaaagacat gaaagaattc ctcgacgaac
    12781 accaaaaaat gaccagcggt aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg
    12841 tgaagtcgat gaaacggcag acttgcccgc tgaattgcct cccttgctgc gccgtttata
    12901 cgccagccgg ggagtacgca gtgcgcaaga actggaacgc agtgttaaag gtatgttgcc
    12961 ctggcagcaa ctaagcggtg tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga
    13021 aggaacgcgg attattgtgg tcggtgattt cgacgccgac ggcgcgacca gcacggctct
    13081 aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc gactacctgg taccaaaccg
    13141 tttcgaagac ggttacggct taagcccgga agtggtcgat caggcccatg cccgtggcgc
    13201 gcagttaatt gtcacggtgg ataacggtat ttcctcccat gcgggagttg agcacgctcg
    13261 ctcgttgggc atcccggtta ttgttaccga tcaccatttg ccgggcgaca cattacccgc
    13321 agcggaagcg atcattaacc ctaacttgcg cgactgtaat ttcccgtcga aatcactggc
    13381 aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc acctttttgc gcgatcaggg
    13441 ctggtttgat gagcgcggca tcgcaattcc taacctggca gaactgctgg atctggtagc
    13501 actggggaca gtagcggacg tcgtgccgct ggacgctaat aatcgcattc tgacctggca
    13561 ggggatgagt cgcatccgtg ccggaaagtg ccgtccaggg attaaagcgc tgctggaagt
    13621 ggcaaatcgc gatgcacaaa aactagccgc cagcgattta ggttttgcgc tgggaccgcg
    13681 tctcaatgct gccgggcgac tggatgatat gtccgttggt gtggcgctgt tgctgtgcga
    13741 caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa
    13801 agagatcgaa caaggaatgc aggttgaagc cctgaccctg tgcgagaaac tggagcgcag
    13861 ccgtgacacg ctacccggcg ggctggcaat gtatcacccc gaatggcatc agggcgttgt
    13921 cggtattctg gcttcgcgta tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc
    13981 agcaggtgac ggtacgctga aaggttccgg tcgctccatt caggggctgc atatgcgtga
    14041 tgcgctggag cgattagaca cactctaccc cggcatgatg ctcaagtttg gcggtcatgc
    14101 gatggcggcg ggtttgtcgc tggaagagga taaattcgaa ctctttcaac aacggtttgg
    14161 cgaactggtt actgagtggc tggccccttc gctattgcaa ggcgaagtgg tgtcagacgg
    14221 tccgttaagc ccggccgaaa tgaccatgga agtggcgcag ctgctgcgcg atgctggccc
    14281 gtgggggcag atgttcccgg agccgctgtt tgacggtcat ttccgtctgc tgcaacagcg
    14341 gctggtgggc gaacgtcatt tgaaggtgat ggtcgaaccg gtcggcggcg gtccactgct
    14401 ggatggtatt gcttttaatg tcgataccgc tctctggccg gataacggcg tgcgcgaagt
    14461 gcaactggct tataagctcg atatcaacga gtttcgcggc aaccgcagcc tgcaaattat
    14521 catcgacaat atctggccaa tttagcgtca tcttctctat aaaaaagagc gtggattggg
    14581 tacaatcccg ctcttatcac cgcattttga ctagctcaat aaaagaaatc agaccatgtt
    14641 tgaaattaat ccggtaaata atcgcattca ggacctcacg gaacgctccg acgttcttag
    14701 ggggtatctt tgactacgac gccaagaaag agcgtctgga agaagtaaac gccgagctgg
    14761 aacagccgga tgtctggaac gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct
    14821 ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca ggggctggaa gatgtttctg
    14881 gtctgctgga actggctgta gaagctgacg acgaagaaac ctttaacgaa gccgttgctg
    14941 aactcgacgc cctggaagaa aaactggcgc agcttgagtt ccgccgtatg ttctctggcg
    15001 aatatgacag cgccgactgt tacctcgata ttcaggcggg gtctggcggt acggaagcac
    15061 aggactgggc gagcatgctt gagcgtatgt atctgcgctg ggcagaatcg cgtggtttca
    15121 aaactgaaat catcgaagag tcggaaggtg aagtggcggg tattaaatcc gtgacgatca
    15181 aaatctccgg cgattacgct tacggctggc tgcgtacaga aactggcgtt caccgcctgg
    15241 tgcgtaaaag cccgtttgac tccggcggtc gtcgccacac gtcgttcagc tctgcgtttg
    15301 tttatccgga agttgatgat gatattgata tcgaaatcaa cccggcggat ctgcgcattg
    15361 acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc
    15421 gtattaccca catcccgacc gggatcgtga cccagtgcca gaacgaccgt tcccagcaca
    15481 agaacaaaga tcaggccatg aagcagatga aagcgaagct ttatgaactg gagatgcaga
    15541 agaaaaatgc cgagaaacag gcgatggaag ataacaaatc tgacatcggc tggggcagcc
    15601 agattcgttc ttatgtcctt gatgactccc gcattaaaga tctgcgcacc ggggtagaaa
    15661 cccgcaacac gcaggccgtg ctggacggca gcctggatca atttatcgaa gcaagtttga
    15721 aagcagggtt atgaggaacc aacatgtctg aacaacacgc acagggcgct gacgcggtag
    15781 tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct ggcgaacctg cgtgagcagg
    15841 gaattgcctt cccgaacgat ttccgtcgcg atcatacctc tgaccaattg cacgcagaat
    15901 tcgacggtaa agagaacgaa gaactggaag cgctgaacat cgaagtcgcc gttgctggcc
    15961 gcatgatgac ccgccgtatt atgggtaaag cgtctttcgt taccctgcag gatgtgggtg
    16021 gccgcattca gctgtacgtt gcccgtgacg atctgccgga aggcgtttac aacgagcagt
    16081 tcaaaaaatg ggacctcggc gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa
    16141 ccggcgaact gtctatccac tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc
    16201 tgccggataa attccacggc ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg
    16261 atctcatctc taacgatgaa tcccgtaaca cctttaaagt acgctcgcag atcctctctg
    16321 gtattcgcca gttcatggtg aaccgcggct ttatggaagt tgaaacgccg atgatgcagg
    16381 tgatcccagg cggtgccgct gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg
    16441 acatgtacct gcgtatcgcg ccggaactgt acctcaagcg tctggtggtc ggtggcttcg
    16501 agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg tatttccgta cgtcataacc
    16561 cagagttcac catgatggaa ctctacatgg cttacgcaga ttacaaagat ctgatcgagc
    16621 tgaccgaatc gctgttccgt actctggcac aggatattct cggtaagacg gaagtgacct
    16681 acggcgacgt gacgctggac ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga
    16741 tcaagaaata tcgcccggaa accgacatgg cggatctgga taacttcgac tctgcgaaag
    16801 cgattgctga atctatcggt atccacgttg agaagagctg gggtctgggc cgtatcgtta
    16861 ccgagatctt cgaagaagtg gcagaagcac atctgattca gccgaccttc attactgaat
    16921 atccggcaga agtttctccg ctggcgcgtc gtaacgacat taacccggaa atcacagacc
    16981 gctttgagtt cttcatcggt ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg
    17041 cggaagatca ggcgcaacgc ttcctggatc aggttgccgc gaaagacgca ggtgacgacg
    17101 aagcgatgtt ctacgatgaa gattacgtta ccgcactgga acatggctta ccgccgacag
    17161 caggtctggg gattggcatc gaccgtatgg taatgctgtt caccaacagc cataccatcc
    17221 gcgacgttat tctgttcccg gcgatgcgtc cggtgaaata agcattacgt tatgctcaca
    17281 accccggcaa atgtcggggt tttttattta agctgggtaa atgcagataa tcgtattctg
    17341 gcttcgcgat ttgtcgcctg catcaccatc cacggactga acgcccacgg cgtggcatca
    17401 ataccgcgta atacatcagc taaatcacac cattgataat ccatcacttc atcatcgttg
    17461 atctgtaacg cactggtggt gcgtgcggca aataccggac acacttcatt ttccacaatg
    17521 cctctcggat cggtggcgcg gtagcgaaag tcaggataga tagattcagg aggcgtaatt
    17581 tccacgccaa gctcataacg gcaacggcgg atcaccgcgt cttcgctgct ttctcccagt
    17641 tgtgggtgcc cacaaaccga gttagtccac acgccaggcc acgctttttt gctcagtgcg
    17701 cggcgggtaa ctaataattg ccccttggca ttaaacagcc aactggagaa cgcgagatgt
    17761 aagagggtgt ctgccgtgtg tgcggcatac ttttccagcg tacccgtggg aactccctgt
    17821 gcattcaata aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc
    17881 atcattatag ataattgaag agtgaaatga ttaaccaaga gagcgtttat cgacaaccgc
    17941 ccgttttgag cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga
    18001 agcgactttc aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa
    18061 gcaacaacag actgaatata gcgcgcatta aaaaattcat attattctcc atgctcattt
    18121 ttcttcagct tgcgcatcag aagccacata cgtactgtcc tgacttctaa atctttatca
    18181 gagacggatt ccttcaccag gtcagcatga tgctgatagc cattaaagaa gacgttcagc
    18241 acaacggcgc taagggttgc gagcataatg ccgctatgta gcagcggttg cagtacggcc
    18301 ggtaacttag aaaagaaatc gtgagagagc gtcggagtca tgccaacgcc gagactgatc
    18361 gccacaatat agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc
    18421 cctgtcgcca gtaccatgcc gaacatcact agaccagcgc cgcccagcac aaattgcgga
    18481 atggaggcta ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc
    18541 gaagaaatac acacccagcg gctatgaacg cgcatcacgc taaccaggcc aacgttttga
    18601 gaaaatgacg tgtgggggaa gctgttaaac gtgccgccta tcattgtccc tacgccatcg
    18661 acacgcagcc cgcaaataat atcgtgtgaa gagagtttac gaccgactat ttcacccagt
    18721 gccaggaaca tccccatcga ctcgataaac acgatgatta acacggcagt catggtcaga
    18781 atggaaacgg gatcgaaaat cggcatacca aacgacatcg gggtgacaat cgcaaaccac
    18841 gaggcatcat gtagcccgga taaattgact tcattcatca tccacgaaag taaaaagcca
    18901 aatacaatcc ccagtaatac ggcgacgttg gacataaatc ctttcgcata gcgagtaatg
    18961 agcaagataa aaattaagac ggcaaaggag atacctaaat aaacgggatt accatattgc
    19021 ggattccctt tacctccagc ggcccagtca atacccacct gaatgatgct aagcccaata
    19081 gaagtaataa ccacaccggt aaccagtggc gggaataaag gcatcaagcg accaataagt
    19141 ggcgctaata atgtggtgat aaaacccgcg gcgatagtgg caccaaatat ccccagcagg
    19201 ccgatatccg ggttcatccc aatggctatc attggtgtta cagcagcaaa ggtcaccgac
    19261 ataatcaccg gcaggcggat ccccataaag cggccgatac cgatacattg caataatgtg
    19321 acgatcccgc agcaaaagag atccgagcta atgagcatcg caatagcatc ttttgagagg
    19381 cccagtcggt caccaatcat aagaggaaca gcgactgcac ctgcgtacat gacaaggacg
    19441 tgttgcagac cgagtatgat cagctttcct ggtgataata tgcggtcaac ctcatcagtt
    19501 gggcggtctt gcccagaaga tgagggaagt tgggaatcta tggcgctcat cagtgtttct
    19561 cctttcgccc gccagaataa aaagcaaccg tttacgttaa tgaaactgtc taaacacatg
    19621 ctcgtggagt gccagttttt gcgaggaagg ggagttagag acaggaattg cggaggcaat
    19681 cacataatcg tgcttaaacc acgaaaatga caagagaaac tgacaatgga attatctgta
    19741 attttctcat attgatattt cattaatcat tctggcatgt cgcactctcg catttaatcg
    19801 tttttatctg gatagcgctc ttttgatcgg caatgttgaa ataatgaaag cggcatagat
    19861 tgaggggcaa taatttgaac tcagtcgcaa aattataatt aaaacggtca tgctacctaa
    19921 tatatgcccg tttattgcat cgcgaaattt gagaagacat aactcattaa atgaaaggga
    19981 tgcaaatgaa tacgtttatc gctgctgaag ctgcggaatg tataggctgt c
//
LOCUS       DAZPFP010000058        20019 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPFP010000058 DAZPFP010000000
VERSION     DAZPFP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.058"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..974
                     /gene="ybeQ"
                     /locus_tag="ACHUR4_002007"
     CDS             <1..974
                     /gene="ybeQ"
                     /locus_tag="ACHUR4_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1455601.1"
                     /translation="FTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSPDD
                     EKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASNAL
                     GWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYKQA
                     ALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGV
                     DQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATA
                     WYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(1044..1526)
                     /locus_tag="ACHUR4_002008"
     CDS             complement(1044..1526)
                     /locus_tag="ACHUR4_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1455602.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            1761..4343
                     /gene="leuS"
                     /locus_tag="ACHUR4_002009"
     CDS             1761..4343
                     /gene="leuS"
                     /locus_tag="ACHUR4_002009"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1455603.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4358..4939
                     /gene="lptE"
                     /locus_tag="ACHUR4_002010"
     CDS             4358..4939
                     /gene="lptE"
                     /locus_tag="ACHUR4_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1455604.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            4939..5970
                     /gene="holA"
                     /locus_tag="ACHUR4_002011"
     CDS             4939..5970
                     /gene="holA"
                     /locus_tag="ACHUR4_002011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1455605.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5972..6613
                     /gene="nadD"
                     /locus_tag="ACHUR4_002012"
     CDS             5972..6613
                     /gene="nadD"
                     /locus_tag="ACHUR4_002012"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1455606.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6637..7248
                     /locus_tag="ACHUR4_002013"
     CDS             6637..7248
                     /locus_tag="ACHUR4_002013"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR1455607.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7508..7825
                     /gene="rsfS"
                     /locus_tag="ACHUR4_002014"
     CDS             7508..7825
                     /gene="rsfS"
                     /locus_tag="ACHUR4_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1455608.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7829..8296
                     /gene="rlmH"
                     /locus_tag="ACHUR4_002015"
     CDS             7829..8296
                     /gene="rlmH"
                     /locus_tag="ACHUR4_002015"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1455609.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8327..10228
                     /gene="mrdA"
                     /locus_tag="ACHUR4_002016"
     CDS             8327..10228
                     /gene="mrdA"
                     /locus_tag="ACHUR4_002016"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1455610.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10231..11343
                     /gene="mrdB"
                     /locus_tag="ACHUR4_002017"
                     /gene_synonym="rodA"
     CDS             10231..11343
                     /gene="mrdB"
                     /locus_tag="ACHUR4_002017"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1455611.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11354..12442
                     /gene="rlpA"
                     /locus_tag="ACHUR4_002018"
     CDS             11354..12442
                     /gene="rlpA"
                     /locus_tag="ACHUR4_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1455612.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12581..13792
                     /gene="dacA"
                     /locus_tag="ACHUR4_002019"
     CDS             12581..13792
                     /gene="dacA"
                     /locus_tag="ACHUR4_002019"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1455613.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13903..14166
                     /gene="ybeD"
                     /locus_tag="ACHUR4_002020"
     CDS             13903..14166
                     /gene="ybeD"
                     /locus_tag="ACHUR4_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1455614.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14267..14908
                     /gene="lipB"
                     /locus_tag="ACHUR4_002021"
     CDS             14267..14908
                     /gene="lipB"
                     /locus_tag="ACHUR4_002021"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1455615.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15167..16120
                     /locus_tag="ACHUR4_002022"
     CDS             15167..16120
                     /locus_tag="ACHUR4_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1455616.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            16329..17294
                     /gene="lipA"
                     /locus_tag="ACHUR4_002023"
     CDS             16329..17294
                     /gene="lipA"
                     /locus_tag="ACHUR4_002023"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1455617.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17395..17598)
                     /gene="tatE"
                     /locus_tag="ACHUR4_002024"
     CDS             complement(17395..17598)
                     /gene="tatE"
                     /locus_tag="ACHUR4_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1455618.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17727..18515)
                     /locus_tag="ACHUR4_002025"
     CDS             complement(17727..18515)
                     /locus_tag="ACHUR4_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1455619.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            18608..18991
                     /gene="crcB"
                     /locus_tag="ACHUR4_002026"
     CDS             18608..18991
                     /gene="crcB"
                     /locus_tag="ACHUR4_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1455620.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(19045..19254)
                     /gene="cspE"
                     /locus_tag="ACHUR4_002027"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19045..19254)
                     /gene="cspE"
                     /locus_tag="ACHUR4_002027"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1455621.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19429..19989)
                     /gene="pagP"
                     /locus_tag="ACHUR4_002028"
     CDS             complement(19429..19989)
                     /gene="pagP"
                     /locus_tag="ACHUR4_002028"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1455622.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5043 a         5067 c         5038 g         4871 t
ORIGIN      
        1 tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa
       61 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
      121 actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag caaggtcatt
      181 gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata
      241 atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
      301 acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa gcggttacct
      361 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt ggatggatgt
      421 acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg tacaaacaag
      481 ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat aaagacggag
      541 aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc gcacagcagg
      601 gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
      661 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg
      721 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
      781 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
      841 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
      901 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
      961 aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc atattctctt
     1021 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcataa
     1081 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
     1141 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
     1201 ttgaggtcct ggaaaatttc gcgccattca agctgcgttt tatcggtgat gtctgccagc
     1261 tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg
     1321 ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca
     1381 tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
     1441 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc gaccagttca
     1501 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
     1561 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
     1621 cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
     1681 ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga
     1741 aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag
     1801 tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga
     1861 agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac
     1921 gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc aaaaacgttc
     1981 tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca
     2041 acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa
     2101 tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc
     2161 gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat aaaaagactt
     2221 ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg
     2281 gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca
     2341 aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat cactggccag
     2401 acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct
     2461 tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta
     2521 tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa
     2581 ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg
     2641 aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa
     2701 cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg
     2761 cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc
     2821 tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt tctcagcaag
     2881 ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt gaccatgaag
     2941 cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga
     3001 actaccgcct gcgcgactgg ggtgtttctc gccagcgtta ctggggcgcg ccgattccga
     3061 tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg ccggtgatcc
     3121 tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg
     3181 caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta
     3241 tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg
     3301 attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg
     3361 ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg
     3421 tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct
     3481 tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg
     3541 aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt
     3601 ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg
     3661 ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata
     3721 tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct
     3781 ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc
     3841 tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct aaagtgaccg
     3901 atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga
     3961 acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg caggaagcac
     4021 tgcaggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc acgctgtggc
     4081 aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag
     4141 cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa
     4201 tcaccgtccc ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc
     4261 tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta ccaggtaaac
     4321 tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacactgtt
     4381 gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg acaccacgca
     4441 ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag
     4501 ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac
     4561 gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc
     4621 ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt
     4681 gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cattcttcga
     4741 taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta
     4801 cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg
     4861 ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc
     4921 caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat
     4981 gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc
     5041 caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca cactttttcc
     5101 attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt
     5161 gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat
     5221 gaacaacttc tcacactcac cggacttctg catgacgacc tgctattgat cgtccgcggt
     5281 aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc
     5341 gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgtgcgc
     5401 gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg ctactgttat
     5461 gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac
     5521 ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct
     5581 tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag
     5641 caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca acgtgaactg
     5701 ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat
     5761 aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt
     5821 cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa
     5881 gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa
     5941 cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg
     6001 cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg cgaatttgat
     6061 tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga
     6121 agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt
     6181 tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa
     6241 agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg gtcaggattc
     6301 attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat
     6361 cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct
     6421 ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta
     6481 tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa
     6541 cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta accaacaagg
     6601 cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat
     6661 ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac acccctgacc
     6721 gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt ttcctttgac
     6781 ttggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac
     6841 cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag
     6901 atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat
     6961 gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg
     7021 gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt aatcgtcagc
     7081 catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc cgagtctatg
     7141 tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca aaaattcgcg
     7201 acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga acgcatgact tttctgtttt
     7261 tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt ctccgcagcc
     7321 agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc
     7381 tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg
     7441 aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat tcacccaggg
     7501 ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa
     7561 aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat
     7621 catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga
     7681 gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat
     7741 tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta
     7801 tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa
     7861 aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat
     7921 gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg acatcaagcg
     7981 catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac
     8041 cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg
     8101 gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt tgtcgcctgc
     8161 ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc cccatccgct
     8221 ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc
     8281 ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa
     8341 ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt
     8401 tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt
     8461 tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc
     8521 gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca accgcactat
     8581 ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc tggacgcttt
     8641 gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg
     8701 ttcacaccgt ttcacctcta ttccggtgaa aactaaccta accgaagtac aagtagctcg
     8761 ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta
     8821 ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa
     8881 agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat
     8941 tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga
     9001 agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca
     9061 agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct
     9121 gctggcgggt agccgcgcag ctgtggttgt taccgaaccg cgtacaggtg gggtgctggc
     9181 gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga
     9241 ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt
     9301 ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa gcgccggggt
     9361 gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag ggtcggaaaa
     9421 acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga
     9481 agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc
     9541 cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg
     9601 ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca
     9661 gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc caatccagat
     9721 gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc atttgctgat
     9781 gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac cgcccgtcgg
     9841 cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg
     9901 ccctaatggt acggcgaata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc
     9961 tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc
    10021 cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt
    10081 ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta cgctgatgcg
    10141 ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc
    10201 agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc
    10261 tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt gctggtttac
    10321 agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaatc
    10381 ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt
    10441 tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat
    10501 gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat tgttcgtttt
    10561 cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc
    10621 gacgtttgcc cgccatcgtt gaagaatact ggcatcgcgc tggtgctgat atttatgccc
    10681 acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt
    10741 ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg
    10801 gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc
    10861 gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag
    10921 tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag
    10981 tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt actggcggaa
    11041 gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc
    11101 gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta
    11161 atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg
    11221 gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg
    11281 gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg
    11341 taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc
    11401 ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg
    11461 taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac
    11521 ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg
    11581 atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc
    11641 ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc ttccgatccc
    11701 cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga
    11761 tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct
    11821 taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc
    11881 gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc
    11941 acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat
    12001 tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag
    12061 tagcggtttc ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc
    12121 gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa cctcgcctgc
    12181 aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg
    12241 ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg
    12301 cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc
    12361 cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc
    12421 atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg
    12481 acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg
    12541 gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    12601 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    12661 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    12721 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    12781 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    12841 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    12901 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    12961 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat
    13021 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    13081 cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca
    13141 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    13201 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    13261 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    13321 caaagcaggt tacaatcttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    13381 ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    13441 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    13501 accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    13561 gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    13621 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    13681 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    13741 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    13801 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    13861 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    13921 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    13981 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    14041 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    14101 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    14161 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    14221 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    14281 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg ctatgcatga
    14341 attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg agcactatcc
    14401 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    14461 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    14521 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    14581 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    14641 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    14701 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    14761 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    14821 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    14881 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    14941 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    15001 tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg attaacctat
    15061 tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt
    15121 aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa
    15181 tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac
    15241 cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg
    15301 gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca
    15361 gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc
    15421 taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg
    15481 cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc
    15541 ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca
    15601 gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac
    15661 cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt
    15721 caccgacaat atggtgttaa tttgccgcaa gggaaatcca ctactctctt tagaagatga
    15781 cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagggc aaaatttcag
    15841 cggtctgcgg cagagagttc aagagatgtt tccggaccgg caaatcaatt tcaccagcta
    15901 caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag
    15961 ccgtttttat aacctgttta gtcgttgctg gccgctggaa aaactgcctt ttccgtcctt
    16021 aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcatcctgc gtgatcccat
    16081 cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag
    16141 atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg
    16201 ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt
    16261 tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca
    16321 cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata
    16381 agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc
    16441 cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg
    16501 caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg
    16561 aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt
    16621 gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga
    16681 aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc
    16741 gtgatgacct gcgcgatggc ggtgcccaac actttgcgga ttgtattact gccattcggg
    16801 aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgaatggatc
    16861 gtgctctgga tattctgact gcaacgcccc cagatgtgtt caaccataac ctggaaaacg
    16921 taccgcgtat ttaccggcag gtacggcctg gcgcagatta caactggtcg ctgaagctgc
    16981 tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc
    17041 tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga
    17101 cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt
    17161 acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc
    17221 atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggaa
    17281 tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt
    17341 gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc
    17401 tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc
    17461 gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt
    17521 acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt
    17581 aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta
    17641 aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg
    17701 tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg
    17761 tttaagatgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata
    17821 atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg
    17881 ctttgcccga tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc
    17941 agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc acgaacccag
    18001 gcggcaggaa gtaccaggat ttcagctccc tgtaaagcct gtgccagcgc cagctctgga
    18061 aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc cagtaacgga
    18121 gctatttcat taccagcatc aacatggcgt gattcctgaa tggcaaatgc atcatagaga
    18181 tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag catattccat
    18241 gcgcgccccg gcgttgaagg aagatgaatc gtcagaattg tcgtcatcat gttacgttta
    18301 ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat
    18361 agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacaaa cagcgatacg
    18421 tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca
    18481 gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa
    18541 attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
    18601 ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtagc
    18661 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
    18721 ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
    18781 gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
    18841 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
    18901 ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgaccg cactggcatt
    18961 ctggctgttt tcggcctcaa ccgtacacta aagaccaaaa aaaacccgct gattaagcgg
    19021 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
    19081 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
    19141 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
    19201 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
    19261 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt atcagtacac caattgtggt
    19321 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgttg
    19381 cttattttag ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
    19441 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
    19501 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
    19561 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
    19621 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
    19681 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
    19741 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
    19801 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
    19861 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc ctctcatctg cgttagcaaa
    19921 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
    19981 cacgttcatt tgtgaccata aaacatttat caaaaatct
//
LOCUS       DAZPFP010000059        19774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPFP010000059 DAZPFP010000000
VERSION     DAZPFP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.059"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHUR4_002029"
     tRNA            complement(2..94)
                     /locus_tag="ACHUR4_002029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUR4_002030"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUR4_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1455623.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUR4_002031"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUR4_002031"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1455624.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUR4_002032"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUR4_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1455625.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHUR4_002033"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHUR4_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1455626.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHUR4_002034"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHUR4_002034"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1455627.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHUR4_002035"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHUR4_002035"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1455628.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACHUR4_002036"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACHUR4_002036"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACHUR4_002037"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACHUR4_002037"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1455629.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACHUR4_002038"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACHUR4_002038"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1455630.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACHUR4_002039"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACHUR4_002039"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR1455631.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="ACHUR4_002040"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="ACHUR4_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1455632.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACHUR4_002041"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACHUR4_002041"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1455633.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHUR4_002042"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHUR4_002042"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1455634.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHUR4_002043"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHUR4_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1455635.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACHUR4_002044"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACHUR4_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1455636.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACHUR4_002045"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACHUR4_002045"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1455637.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHUR4_002046"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHUR4_002046"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1455638.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHUR4_002047"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHUR4_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1455639.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4653 a         5513 c         5034 g         4574 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agttgcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatc
//
LOCUS       DAZPFP010000060        19770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPFP010000060 DAZPFP010000000
VERSION     DAZPFP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.060"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUR4_002048"
     CDS             <1..122
                     /locus_tag="ACHUR4_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1455640.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHUR4_002049"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHUR4_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1455641.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHUR4_002050"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHUR4_002050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1455642.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUR4_002051"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUR4_002051"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1455643.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUR4_002052"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUR4_002052"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1455644.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUR4_002053"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUR4_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1455645.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUR4_002054"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUR4_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1455646.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUR4_002055"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUR4_002055"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1455647.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUR4_002056"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUR4_002056"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR1455648.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUR4_002057"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUR4_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR1455649.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUR4_002058"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUR4_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR1455650.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUR4_002059"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUR4_002059"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR1455651.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUR4_002060"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUR4_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR1455652.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHUR4_002061"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHUR4_002061"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR1455653.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUR4_002062"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUR4_002062"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1455654.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUR4_002063"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUR4_002063"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR1455655.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUR4_002064"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUR4_002064"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1455656.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHUR4_002065"
     CDS             18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHUR4_002065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR1455657.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19444..>19770
                     /gene="wzx"
                     /locus_tag="ACHUR4_002066"
     CDS             19444..>19770
                     /gene="wzx"
                     /locus_tag="ACHUR4_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR1455658.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLK"
BASE COUNT         4936 a         4744 c         5356 g         4734 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgattatt ttatatgata aagaaatgta
    19441 ataatgagca taataaaaaa tagtgtctgg aacctttttg gctatgcaat accaacttta
    19501 attgctatcc cctcgctagg atttctcgct cgaggtttgg ggcctgaagg tttcggtgtt
    19561 tatacaattg caattgcact tgttgggtat gctggaattt ttgatgtagg cctgactcgc
    19621 tctgttatta gagaaattgc aattcatcgt gataatcatc atgaaagaac caaggtaatt
    19681 tcaacaagta catctttttt ggtgctattt tcatgctttg gtgctttttt attattgatt
    19741 ttctctgatg gaattgttaa ttatctaaaa
//
LOCUS       DAZPFP010000061        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPFP010000061 DAZPFP010000000
VERSION     DAZPFP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.061"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUR4_002067"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUR4_002067"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1455659.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUR4_002068"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUR4_002068"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1455660.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUR4_002069"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUR4_002069"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1455661.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHUR4_002070"
     CDS             6038..6658
                     /locus_tag="ACHUR4_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1455662.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHUR4_002071"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHUR4_002071"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1455663.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUR4_002072"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUR4_002072"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1455664.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUR4_002073"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUR4_002073"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1455665.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUR4_002074"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUR4_002074"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1455666.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHUR4_002075"
     CDS             complement(10068..11069)
                     /locus_tag="ACHUR4_002075"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1455667.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUR4_002076"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUR4_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUR4_002077"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUR4_002077"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1455668.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUR4_002078"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUR4_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1455669.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUR4_002079"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUR4_002079"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1455670.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUR4_002080"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUR4_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1455671.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUR4_002081"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUR4_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1455672.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUR4_002082"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUR4_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1455673.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZPFP010000062        19231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPFP010000062 DAZPFP010000000
VERSION     DAZPFP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.062"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            103..1686
                     /locus_tag="ACHUR4_002083"
     CDS             103..1686
                     /locus_tag="ACHUR4_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1455674.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2138..3991)
                     /gene="asmA"
                     /locus_tag="ACHUR4_002084"
     CDS             complement(2138..3991)
                     /gene="asmA"
                     /locus_tag="ACHUR4_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1455675.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4013..4594)
                     /gene="dcd"
                     /locus_tag="ACHUR4_002085"
     CDS             complement(4013..4594)
                     /gene="dcd"
                     /locus_tag="ACHUR4_002085"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1455676.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4686..5327)
                     /gene="udk"
                     /locus_tag="ACHUR4_002086"
     CDS             complement(4686..5327)
                     /gene="udk"
                     /locus_tag="ACHUR4_002086"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1455677.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5645..5887
                     /locus_tag="ACHUR4_002087"
                     /pseudo
     CDS             5645..5887
                     /locus_tag="ACHUR4_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            5886..6827
                     /gene="mdtA"
                     /locus_tag="ACHUR4_002088"
                     /pseudo
     CDS             5886..6827
                     /gene="mdtA"
                     /locus_tag="ACHUR4_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            6827..9949
                     /gene="mdtB"
                     /locus_tag="ACHUR4_002089"
                     /pseudo
     CDS             6827..9949
                     /gene="mdtB"
                     /locus_tag="ACHUR4_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            9950..13015
                     /gene="mdtC"
                     /locus_tag="ACHUR4_002090"
     CDS             9950..13015
                     /gene="mdtC"
                     /locus_tag="ACHUR4_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1455678.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLPALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            13016..14431
                     /locus_tag="ACHUR4_002091"
     CDS             13016..14431
                     /locus_tag="ACHUR4_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455679.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            14428..15831
                     /gene="baeS"
                     /locus_tag="ACHUR4_002092"
     CDS             14428..15831
                     /gene="baeS"
                     /locus_tag="ACHUR4_002092"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1455680.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            15828..16550
                     /gene="baeR"
                     /locus_tag="ACHUR4_002093"
     CDS             15828..16550
                     /gene="baeR"
                     /locus_tag="ACHUR4_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1455681.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            16741..17073
                     /locus_tag="ACHUR4_002094"
     CDS             16741..17073
                     /locus_tag="ACHUR4_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1455682.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            17220..18581
                     /gene="yegQ"
                     /locus_tag="ACHUR4_002095"
     CDS             17220..18581
                     /gene="yegQ"
                     /locus_tag="ACHUR4_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1455683.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(18923..>19231)
                     /locus_tag="ACHUR4_002096"
     CDS             complement(18923..>19231)
                     /locus_tag="ACHUR4_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455684.1"
                     /translation="IAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDNQYY
                     TEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE
                     "
BASE COUNT         4326 a         5199 c         5162 g         4544 t
ORIGIN      
        1 ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga
       61 attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat
      121 ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat
      181 aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca
      241 cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctagc gtcaatctcc
      301 tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt tagcgcccgc
      361 gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac
      421 gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg
      481 ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc agtgattacc
      541 gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc
      601 ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt
      661 attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc
      721 ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggca
      781 ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaatca gacgctacgc
      841 cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg
      901 gacgccaaaa ccgcgtccat gctggtggat catggaaacc agcagatctt taatccgcag
      961 gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtaag cagcattatg
     1021 acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga aatccgccag
     1081 ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat
     1141 ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt cactgcgcgg cgaaccgctc
     1201 aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacca
     1261 gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga tgagtttggc
     1321 tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg
     1381 aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg
     1441 acggcgaatg gtcatatgcc gctggaggat ctggtgcaat atgtgccgct gccgctggat
     1501 gaaaaacgtg aatatcacac cattgccggg ctgttgatgg agtatttgca gcgtattcca
     1561 aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc
     1621 catcgcgtgc agaaggtgca gattataccg ctgcgtaagg atggcgagat ggagtacgag
     1681 gtgtgatggt atcggccatt ttacccaatg ctgcgtaacg ccttataact tcagcaatga
     1741 agcgttgtct gtggtaaacg gacatcacag cgggggtgtg ggtccggctg taaatcgccg
     1801 aatcgtttcc tgtaggctgg ggcagcccac atggatgtgg gctgagggcg cgttttacag
     1861 ggatgttacc tcgcgcccgt acccgatagc cgaaaggaat aagatgaggg aaccgcgcca
     1921 gcggcgattt accgccggga gcccgggttg ccagggtggt ggcgattgag ccaccctggc
     1981 acgttcaccg gcttgagcgt tccatagtag cgaggaacat caagtgaacg gaacagcctt
     2041 cgatcttaca tattcagaat ataatagtgc cgccgcgaac gcctgatgcg acgctttacg
     2101 cgtcttatca ggcctacagg acaggtcacc tggtaaccta catcttctcc agcaacttct
     2161 tcacatcttt gccattgcgg gaatctttat tccgctcggc ccagtcattc aggcgacgtt
     2221 tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac ttgcaggctg taattgagtt
     2281 gctgccagtt gccataaacc cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt
     2341 tgctttcccc gttccagcca cccacgaccc gaatatcaaa ctgggtgtcg caggtttgat
     2401 ctgccagatt caacatgcct tcccctgtca gcgccagcac aggcgattga ccttgcatgt
     2461 cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg tcaagccgcg
     2521 tcacgttatc gaagttttca gcggccttca catcaccgcc attacgctct accgcttgct
     2581 gaatcatctg ctggaagttc atcccttcca tacgtgtgtc ggtcatctca acatgcgcct
     2641 gtccttgcca gttgtggcgg aatgcgtcgg catctatgtc agcaccggag aaatcaccag
     2701 ccagtgacat ttttccggtc aacgaaatcg gatagttaaa cgccttcaga atggtaccaa
     2761 tctcaacgtt ctccagccgt ggctggaagt ttatccgcgg atttattgat gtcgcgtcca
     2821 gcgtgcccgg cagtgaaacc tttccaccgt taagcttgcc ctgcagttga gtaatttcca
     2881 gcaaacccga cttgttggtc atttgcgtgg caacatctgt aaaattcatt ccgcgccagc
     2941 gcacgttact ggcctgcaac aaaatatcag ctgtaaagcc ttgcagtccc tgataggccg
     3001 gttcatcaat acgcgaagaa atgaccgggc gcggcaacgt tgattggctc tgcccctgct
     3061 gcgcggcacc gttttcacca ttcgcggttt cattaagcgg gatgaggttg tcaagattca
     3121 gttgcgggaa ttgcagtctc agctgccatt ccggtttctc tgtcagcgtg acctgtgctt
     3181 gtccgctcag cgtactgtca ttggcggtca aactaatttg gttaaatgaa aggcgtttat
     3241 gtgactcctg ccactgggac tggaaactcc cctgcccctg aataccttgt tttggtaaat
     3301 cggcaccctg caactgccag ttaatttgtt caatagccgc tgttaaatca tgcggataat
     3361 cagaagcatc taccgtaccg ttaagggata tcgtgagatc gcgctgatcg cgattaaccc
     3421 gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc aggcggatat
     3481 tgcggattgt cacctgctcg tcatcttcat gctggaacac cagaacgctg tccgccacct
     3541 taagactgga tatatcaaac gaccatccgc gatcgtctga cagatccggc aaggtattgt
     3601 cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc gtcagttgga
     3661 tcactgcccc ttttagcatc acctgcttaa cgctcagttg atgactcagt agtggtaaaa
     3721 gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg ctggcttgcg ccctgggcgg
     3781 tgagagacat tcgcccggag aggatactaa gctgcggcca gacgtgccaa cgcagtggcc
     3841 cgtcgagctg caattgataa ccgctacgtg cagcaacttg cttgaccata tagtcgcgga
     3901 aatcattcgg attcaccagc aacactaacg cagataaccc ggccaccagc acgaccaaga
     3961 gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg aattagtctt
     4021 tatcgattcg gctggctacc gcgccctgct ggttgcgata tttcgcatct tcacggcggt
     4081 tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct cagcgcacca attaacatgc
     4141 ccggacgcag cgccagcggc agcttaccgg agttgtagaa ctccagcaca atgcaaccag
     4201 accagcccgg atcgatacgg tgcgcggtga cgtgcaccat cagccccaga cgcgccaaag
     4261 aggaacgccc gtccagccag cccaccagat cggctggcag cgtcaccgac tccagtgtca
     4321 ccgccagcgc cagctctcct gggtgaagat agaacgcctc gccctcgtcg agaacgatct
     4381 catcgctcat cacgcggtca agcgcggcgc tcacttcatc tttgggaccg ctcagatcga
     4441 taaacgctgc cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt acatccaccg
     4501 tcgcgccgtt aatacgctcc actggcggac gtgggttgat cgacaaacgg ccttcatcaa
     4561 gccaggcttc aatatctcgg tcacacagac gcatggcatt ttctcctttc gcacatcact
     4621 cccttaacgc caattacgtc aagggcatac caacttatca ctgaacggta cacaatttat
     4681 caagcttatt caaagaactg acttattttc gctttcaata tatcgatcgc gatgcggttt
     4741 ttcccgccgc gcggcacgat aatgtccgca tattgtttag aaggctcaat gaattgcagg
     4801 aacatcgggc gcacggtttt ttgatattgc gccatcacgg aatccattga acgcccacgc
     4861 tcgttaacgt cacgcttgat gcggcgcatc aggcagatat ccagcggggt atcaacgaaa
     4921 atggagaagt taagttcgtc acgcaaacgc gcatccgtca gcaacaaaat gccttcgaga
     4981 atgatgacct tcttcggctc aaccgtcacc gtttctttca tacgcgtatg ttcaacatag
     5041 ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg ctcaagcaac
     5101 aggctgtgat ccatcgcgct gggatggtcg tagttggtct taacgcgttc ttccatcgac
     5161 agatggcttt gatctttgta atagcagtct tcgggaatta cgccgatgtg ttcatcaccg
     5221 acttgctcac gtaattcacg ataaagggta ctggcaataa gactcttgcc ggaagccgat
     5281 gcgccagcga taccgataat gacgcactga tgagactgat cagtcatata tttagcgacc
     5341 tgattaacct ggatgttagg aagggggcga cgaagcgcca aacgcggcaa ttatagggat
     5401 ttcatccgtc tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacaa
     5461 agaattcccg gtggcaaatt acgttgatca gttttatgta aggtaaaaaa tgttatacgc
     5521 agttgcgcaa attatccgcc tttacgtcac tttatgagca attcgcatat aaaatgtaaa
     5581 acttttgtac tagcataaac acagaaacga atactggcga cctggccttg cggataaagc
     5641 ggtaataagc aaacaatcac agcatgtatt aattgccctg ccccacccgc tgcttcacct
     5701 ggtcagttta ggtttagtct cgtttatctt tacccttgtc tcgcttgagc tttcgcagtt
     5761 tggcacccaa ctcgccccac tgtggttccc gacgtccatc atgatggtgg cgttttatcg
     5821 ccatgccggg cgcatgtggc cgggaattgc gctgagctgt tcgctgggaa atatcgccgc
     5881 atccattcca ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca
     5941 gccagttcaa agttgcatta gcacaaaccc agggccaact ggcaaaagat aaagccacgc
     6001 ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtct
     6061 cccgtcagga gctggatgcc caacaggcgc tggtcagtga aaccgaaggc accattaagg
     6121 ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga ctggagccgc atcaccgcac
     6181 cagtcgatgg tcgcgttggt ctcaagcaag ttgatgttgg taaccaaatc tccagtggtg
     6241 ataccaccgg aattgtggtg atcacccaga cgcatcctat cgatttagtc tttaccctgc
     6301 cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc cggaaaaccg ctgatggtag
     6361 aagcctggga tcgcaccaat tcgaagaaat taagtgaagg cacgctgtta agcctcgata
     6421 accaaatcga tgccacaacc ggtacgatta aagtgaaagc acgctttaat aatcaggatg
     6481 atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg
     6541 ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga aggccatttt gtctgggtgc
     6601 tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc gggcattcag gacagtcaga
     6661 aagtggtgat ccgtgcaggt atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc
     6721 gcctgaccga aggggcgaaa gtggaagtgg tggaagccca gagcgccacc actccggaag
     6781 agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc
     6841 cccgagcagc acaggcggcc cgtcgcgcct gtttattatg cgtcctgtcg ccaccacgct
     6901 gctgatggtg gcgatcttac tcgccgggat tatcggttat cgcgccctgc ccgtttcggc
     6961 gctgccggaa gtggactatc cgaccattca ggtggtcacg ctctacccag gtgccagccc
     7021 ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg
     7081 cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt tgcagttcca
     7141 gctaacatta ccgctcaatg tcgccgagca ggaagtgcag gccgcgatta acgctgcgac
     7201 caacttgttg ccgagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga
     7261 tccgccgatc atgacgctcg ccgtcacctc aaccgccatg ccgatgacgc aagtggaaga
     7321 tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtcg gcctggtgac
     7381 gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc
     7441 cctcggcctg accagcgaaa ccgtgcgcac cgccattacc ggcgctaacg ttaactcggc
     7501 aaaaggtagc ctcgacggcc cttcccgtgc ggtcacgctt tccgcgaacg accagatgca
     7561 atccgccgaa gagtatcgcc agctaatcat cgcctaccag aacggcgcgc caattcgtct
     7621 gggcgatgtc gcaactgtag agcaaggtgc agaaaacagc tggctcggcg cgtgggcgaa
     7681 caaagaacag gccattgtga tgaatgttca gcgccagccc ggtgctaaca ttatctccac
     7741 cgccgacagc attcggcaga tgctgccaca gctcactgag agtctgccga aatcggtgaa
     7801 ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca tccgtcgatg atactcagtt
     7861 tgaattgatg atggctatcg cgctggtagt catgattatc tacctgtttt tgcgcaatat
     7921 tccggcgacc atcattcccg gtgttgctgt accgctgtcg ttaatcggca ctttcgcggt
     7981 tatggtgttt ctcgattttt caatcaataa cctgacactg atggctttaa ctatcgccac
     8041 cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa
     8101 aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt gaaatcggct ttaccattat
     8161 ctcgctgacc ttctcactga ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt
     8221 cgggcgactg ttccgcgaat ttgctattac cctggcggta gcgattttga tctcagcggt
     8281 ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg ctcagccagg agtcgttgcg
     8341 taaacagaac agcttctccc gtgcctcgga aaaaatgttc gacaggataa tcgccgccta
     8401 tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg accttaagcg tggcactcag
     8461 cacgctgctg cttagcgtgc tgctgtgggt gttcattccg aaaggtttct tcccggtaca
     8521 ggacaacggc attattcagg gcactttgca ggcaccgcaa tccagctcct ttgccaatat
     8581 ggcccagcga caacgccagg tcgcggacgt gattttgcag gatccggcag tgcaaagcct
     8641 gacctcattt gttggcgttg atggcactaa cccgtcgctg aatagtgcac gtttacaaat
     8701 caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa aaagtcatcg cccgtctgca
     8761 aacggcggta gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggacctgac
     8821 catcgatact caggtcagcc gcacccagta ccagtttacc ttgcaggcca cgtcactgga
     8881 tgcgctcagt acctgggtgc cacagttgat ggaaaaactc cagcaactgc cacagctttc
     8941 tgatgtctcc agcgactggc aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga
     9001 cagcgccagc cgtctgggga tcagcatggc ggacgtcgat aacgccctgt acaacgcgtt
     9061 tggtcagcga ctgatttcca ctatttatac tcaggctaac cagtatcgcg tggtgctgga
     9121 gcacaatacc gaaaataccc caggcctcgc ggcgctggat accattcgcc tgaccagcag
     9181 cgacggtggc gtggtgccgt taagctcaat tgccaaaatt gagcagcgtt ttgcgccgct
     9241 ttccatcaac catctggatc agttcccggt aacaaccatc tcctttaacg tgccggataa
     9301 ctattcgctg ggcgatgcgg tgcaggcgat tatggacacc gaaaagacgc tgaatctgcc
     9361 ggtggatatc accacgcaat tctagggcag caccctcgcc ttccagtcgg cgctgggcag
     9421 cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta
     9481 cgagagcttt attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc
     9541 gctgctggca ttaatgattg ccggtagcga actggatgtg attgcgatta tcggcattat
     9601 tttgctgatc ggtattgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc
     9661 tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt
     9721 tcgtccgatc ctgatgacca ccctggcggc tctgcttggc gcgctgccgc tgatgttgag
     9781 taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat
     9841 tgtcagccag gtgctgacgc tgttcaccac gccggtgatt tatctgctgt tcgaccgcct
     9901 ggcattgtgg accaaaagtc gctttgctcg ccatgaagag gaggcgtaag tgaagttttt
     9961 tgccctcttc atttaccgcc cggtggcgac gattttactg tcggtcgcca ttaccctgtg
    10021 cggcatactg ggtttccgta tgctgccggt cgccccgctg ccgcaggtcg attttccggt
    10081 gattatggtc agcgcctcgc tgcccggtgc gtcgccggag acaatggcgt cttccgttgc
    10141 cacgccgctg gagcgctcac ttgggcgcat tgccggggtt agtgaaatga cctccagcag
    10201 ttcgctcggc agcacgcgca ttattttgca gtttgatttt gaccgggata tcaacggcgc
    10261 agcgcgtgat gtgcaggcgg cgatcaacgc tgcacaaagt ttgctgccca gcgggatgcc
    10321 cagccgcccg acctatcgca aagcgaaccc gtcggatgcg ccaattatga tcctcacgct
    10381 gacatccgat acttattcgc agggtgaact gtacgatttc gcctcgacgc agctggctcc
    10441 gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtc ggtggcagct cgctgcccgc
    10501 cgtgcgcgtc gggctgaccc cgcaggcgct gtttaatcag ggcgtgtcgc tggacgacgt
    10561 acgcactgcc atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg aagatggcac
    10621 tcaccgctgg cagatccaga ccaacgatga actgaaaacc gccgctgaat atcagccgct
    10681 gattattcac tacaacaacg gcggcgcggt tcgtctgggc gatgtggcga cggtgactga
    10741 ctcagtgcag gatgtgcgca acgccgggat gaccaacgcc aaaccggcta ttttactgat
    10801 gatccgcaaa ctgccggaag ccaatattat ccagacggta gacagcatcc gggcaaaatt
    10861 accagagttg caggagacca ttccggcggc gattgatctg caaattgccc aggatcgctc
    10921 ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg ctgattatct cggtggcgct
    10981 ggtgattctg gtggtctttt tattcctgcg ctcgggtcgc gccactatta ttcccgccgt
    11041 tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct
    11101 caataatctt tcgctgatgg cgctcaccat tgctactggt ttcgtggtgg atgacgccat
    11161 tgtggtgctg gaaaacattg cccgtcatct ggaagcgggg atgaaaccac tacaggccgc
    11221 gctgcaaggt actcgcgaag tcggctttac ggtgctgtcg atgagtctgt cactggtggc
    11281 ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc cgactgttac gcgagtttgc
    11341 cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt tccctgacgc taacgccaat
    11401 gatgtgcggc tggatgctaa aagccagcaa gccgcgtgag caaaagcgac tgcgtggttt
    11461 tggccgcatg ttggtagccc tgcaacaagg ctacggcaag tcgctgaaat gggtgctcaa
    11521 tcatacccgt ctggtgggcg tggtgctgct tggcaccatt gcgctgaata tctcgatccc
    11581 gaaaaccttc ttcccggagc aggacaccgg cgtactgatg ggtgggatac aggcggatca
    11641 gagtatttcg tttcaggcga tgcgcggcaa attgcaggat ttcatgaaaa ttatccgtga
    11701 cgatccggca gtggataatg tcaccggctt taccggcggt tcacgagtta acagtgggat
    11761 gatgtttatc accctcaagc cacgcgacga acgcagcgaa acggcgcagc aaattatcga
    11821 ccgtctgcgc gtgaagctgg cgaaagaacc gggggcgaat ctgttcctga tggcggtaca
    11881 agatattcgc gttggtgggc gtcagtcgaa cgccagctac cagtacacgt tgttatccga
    11941 cgacctgccg gcactgcgcg aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc
    12001 ggaactggcg gacgtgaact ccgatcagca ggataacggc gcggagatga atctggttta
    12061 cgaccgcgac accatggcac ggctgggaat cgacgtacag gccgccaaca gtctgttaaa
    12121 taacgccttc ggtcagcggc aaatctcgac catttaccag ccgatgaacc agtataaagt
    12181 ggtgatggaa gtggatccgc gctataccca ggacatcagt gcgctggaaa aaatgttcgt
    12241 tatcaataac gaaggcaaag cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa
    12301 tgccccacta tcggtgaatc atcagggatt atcggcggcc tcgaccatct cgttcaacct
    12361 gccgaccgga aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa tgacccagct
    12421 tggtgtgcct tcgacggtgc gcggcagttt tgccggcacg gcgcaggttt tccaggagac
    12481 gatgaactcg caggtgatcc tgattatcgc cgccatcgcc acggtgtata tcgtgctggg
    12541 tatcctttac gagagttacg tacatccgct gacgattctc tccaccctgc cctcggcggg
    12601 cgttggagcg ctgttggcgc tggagctgtt caatgccccg ttcagcctaa tcgccctgat
    12661 agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc
    12721 gcttgaagcc caacggcacg gtaacctgac gccgcaggaa gctattttcc aggcctgtct
    12781 gctgcgtttt cgcccgatta tgatgaccac cctggcggcg ctgtttggcg cgctgccgct
    12841 ggtattgtcg ggcggtgacg gctcggagct gcggcaaccc ctggagatca ccattgtcgg
    12901 cggtctggtg atgagccagc tcctgacgct gtataccacg ccggtggtgt atctcttttt
    12961 cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac
    13021 agatcttccc gacagcaccc gctggcaatt atggattgtg gctttcggct tctttatgca
    13081 gtcgctggac accaccatcg taaacaccgc ccttccctca atggcgcaaa gcctcgggga
    13141 aagtccgttg catatgcaca tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct
    13201 gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc aatattttct ttaccgccat
    13261 cgtgctgttt actctcggtt cactgttttg cgcgctttcc ggcacgctga acgaactgtt
    13321 gctggcacgc gcgttacagg gcgttggcgg tgcgatgatg gtgccggtcg gcaggttgac
    13381 ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg atgacctttg tcacgttacc
    13441 cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt ctgctggtag aatacgcgtc
    13501 gtggcactgg atctttttga tcaacattcc ggtggggatt atcggtgcga tcgccacatt
    13561 gatgttaatg ccgaactaca ccatgcagac gcggcgcttt gatctttccg gctttttatt
    13621 gctggcggtt ggcatggcgg tattaaccct ggcgctggac ggcagtaaag gtacaggttt
    13681 atcgccgctg gcgattgctg gcctggtcgc agttggcgtg gtggcactgg tgctttatct
    13741 gctgcacgcc agaaataaca accgcgccct gttcagtctg aaactgttcc gcactcgtac
    13801 cttttcgctg ggcctggcgg ggagctttgc cggacgtatt ggcagtggca tgttgccctt
    13861 tatgacaccg gttttcctgc aaattggcct cggtttctcg ccgtttcatg ccggactgat
    13921 gatgatcccg atggtgcttg gcagcatggg aatgaagcga attgtggtac aggtggtgaa
    13981 tcgctttggt tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc tggtcaccct
    14041 gttgtttatg actaccgctc tgttgggctg gtactacgtt ttgccgttcg tcctgttttt
    14101 acaagggatg gtcaactcga cccgtttctc ctccatgaac accctgacgc tgaaagatct
    14161 cccggacaat ctggcgagca gcggaaacag cctgctgtcg atgattatgc aattgtcgat
    14221 gagtatcggc gtcaccatcg ccgggctgtt gctggggctt tttggttcgc agcatatcag
    14281 cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgtttat
    14341 catcgccctt ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtagc
    14401 tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc gacccggtat taccggcaaa
    14461 ctgtttctgg cgattttcgc cacctgcatt gtcttgctga tcagtatgca ctgggcggtg
    14521 cgtatcagtt ttgagcgtgg ctttattgat tacatcaaac acggcaatga acagcgatta
    14581 cagctgttaa gtgatgcgct tggcgagcag tatgcgcagc atggtaactg gcgcttcctg
    14641 cgcaacaacg atcgctttgt ctttcagatc ctgcgttcat ttgaacacga taattcggaa
    14701 gataaacccg gcccgggtat gccgccgcac ggctggcgta cccagttctg ggtggtcgat
    14761 caaaacaaca aagtactggt tggtccgcga gcgccgattc cacctgacgg tacacggcga
    14821 cccattctgg tcaacggtgc ggaagtgggc gcggtgattg cctcccctgt tgagcggctg
    14881 acccgcaata ctgatatcaa tttcgataaa caacagcggc aaaccagctg gctgatcgtc
    14941 gccctggcaa cgttactcgc ggcgctcgcc acctttctgc tggcgcgcgg tttgctggca
    15001 ccggtaaaac gacttgtcga tggcacgcac aaactggcgg cgggcgattt cactacccgc
    15061 gtaacgccca ccagtgaaga tgaactgggc aaactggcgc aagacttcaa ccagctcgcc
    15121 agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa
    15181 ctgcgtacgc cattagcggt gctgcgcggt gaactggaag ccattcagga tggcgtgcgc
    15241 aaattcacgc cggagacggt ggcgtcttta caggcggagg tcggtacact gaccaaactg
    15301 gttgacgatc ttcatcagtt gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca
    15361 ccggtagatt tgatcccact gctggaagtg gcgggcggcg catttcgcga acgattcgcc
    15421 agccgtggcc tgaaactgca attttccctg ccagacagta tcaccgtatt tggcgatcgc
    15481 gaccgtttaa tgcagttatt caataactta ctggaaaaca gcctgcgcta cactgacagc
    15541 ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa cggtgcgcct gacctttgcc
    15601 gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc
    15661 accgaaggtt cccgcaaccg cgccagcggc ggttccgggc tggggctggc gatttgcctg
    15721 aacattgttg aagcccataa tggccgcatt attgccgccc attcgccttt tggcggggta
    15781 agcattacag tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac
    15841 caatcgacga aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctggggcagt
    15901 tgctcattga ttatctgcgt gctgcgagct atgcgccgac gcttatcagc cacggcgatc
    15961 aggtactggc gtatgtgcgc cagacaccac cggatctgat cctgttggat ctgatgctcc
    16021 ctggcaccga tggcctgaca ttatgccggg aaattcgtcg tttttccgat gttccgatcg
    16081 tgatggtgac ggcaaaaatc gaagagatcg accgcctgct ggggctggag attggcgccg
    16141 atgactacat ctgcaagccg tacagcccac gggaagtggt agcgcgtgtc aaaaccattt
    16201 tgcgccgttg taaaccgcaa cgcgaacttc agcaacagga tgccgaaagc ccgttgatta
    16261 tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg
    16321 aatttcgtct gcttaaaacg ctctctcacg aaccaggaaa agtgttctcc cgcgagcaat
    16381 tgctcaatca tctttatgac gactaccgcg tagtaaccga ccgcaccatt gacagccaca
    16441 ttaaaaatct gcgccgcaag ctggaatctc tcgacgccga acagtcattt atccgcgccg
    16501 tttacggcgt tggttaccgc tgggaagccg acgcctgccg catcgtttag ttttagcgac
    16561 attactttgt tagccggaga tggcgttcag acccgatccg gcaacattat tccacgcatg
    16621 gtcagcaaac tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta
    16681 atcagtaaac agcaccttta gatgctgttt tcgataagca atttcaatca aggagtcatt
    16741 atggctggtt ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa
    16801 gcgggcaatg gtgagactat cctcaccagc gagctgtata cctcaaaagc ctctgcggaa
    16861 aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag agcgctatga gaaaaaaacg
    16921 gcaagcaacg gcaaattcta tttcaatctg aaagccgcta atcatcaaat tatcggctcc
    16981 agccagatgt acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat
    17041 ggcacaagcc agacggtgaa agacaatacg taattacgat gccgggcgtg ggttgttggc
    17101 gtccggcgct ttaccagatc ccccctacag cgctacaatg cccgccctta aagtgggggc
    17161 ctcccctaac cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta
    17221 tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg
    17281 cttatggcgc agatgctgtt tatgcgggcc agccgcgtta ctccctacgt gtgcgcaaca
    17341 acgaattcaa ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa
    17401 agttttatgt cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg
    17461 acctgaaacc ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga
    17521 ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg
    17581 ccgtgaactg ggcgacggtg aaattctggc agcaaatggg actgacccgc gtgatcctct
    17641 ctcgcgagct gtcgctggaa gagattgaag agatccataa tcaggtgccg gatatggaga
    17701 tcgagatctt cgttcacggc gcactgtgca tggcctactc cggtcgctgc ctgctctctg
    17761 gctatatcaa caagcgcgac ccgaaccagg gcacctgcac caacgcctgc cgctgggagt
    17821 acaacgtcca ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga
    17881 ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc gccaaccgac aaagtgttta
    17941 tgatcgaaga agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca
    18001 cttacatcat gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa
    18061 tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc tttctactat tgcgcacgca
    18121 ccgcacaggt ttaccgcaaa gctatcgatg acgccgctgc gggaaaaccg ttcgatacca
    18181 gcctgctgga aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc
    18241 gtcatactca cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc
    18301 agtttgttgg tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa
    18361 ataaattctc cgttggcgac agtcttgagc tgatgacgcc gcaaggcaac attaacttta
    18421 cccttgagca catggaaaac gccaaaggcg aagctatgcc ggtcgcacca ggcgatggtt
    18481 atactgtgtg gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctaatgcgta
    18541 atttctccgg ggaaaccacg cgtaaccccc acggtaagtg attaatttcg attatttttc
    18601 ccggatggaa aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc
    18661 gtttcgctga aaaacataat ccataaaatg ctagctgtac caggaaccac ctccttagcc
    18721 tgtgtaatct cccttacacg ggcttatttt ttgtacataa gctgtaaaga aggattgatc
    18781 cctgataacc gtcttccatt gaccacatcg atagaatccc ccttcatagc acaatgcctt
    18841 tcacgtaacg gtatcgtgct cgcccaggtt ccggctacgc acagccagaa cgcgcatgtt
    18901 tgacgcttac aaaaaaatat tctcactctc cacatttgaa tgtcagacga gcgacgccat
    18961 gtaatcctgc accttctgtc ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac
    19021 agaaatgggc tgcatcattt tttactatat tttctgcacc agatattctg cccctggcta
    19081 aagaagcttc ggcttcggtg tagtattggt tatcgagttt acgctgaata ttacttttat
    19141 atgcaagacc aaatttaccg atacttgttt catcattatg cacagcacaa ccagatataa
    19201 gaaaaatact aattaatgat atagcagcta t
//
LOCUS       DAZPFP010000063        19111 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPFP010000063 DAZPFP010000000
VERSION     DAZPFP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.063"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            547..996
                     /gene="alaE"
                     /locus_tag="ACHUR4_002097"
     CDS             547..996
                     /gene="alaE"
                     /locus_tag="ACHUR4_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1455685.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1033..1377)
                     /locus_tag="ACHUR4_002098"
     CDS             complement(1033..1377)
                     /locus_tag="ACHUR4_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1455686.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1529..1858
                     /locus_tag="ACHUR4_002099"
                     /pseudo
     CDS             1529..1858
                     /locus_tag="ACHUR4_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            2106..2351
                     /gene="nrdH"
                     /locus_tag="ACHUR4_002100"
     CDS             2106..2351
                     /gene="nrdH"
                     /locus_tag="ACHUR4_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1455687.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2348..2758
                     /gene="nrdI"
                     /locus_tag="ACHUR4_002101"
     CDS             2348..2758
                     /gene="nrdI"
                     /locus_tag="ACHUR4_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR1455688.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2731..4875
                     /gene="nrdE"
                     /locus_tag="ACHUR4_002102"
     CDS             2731..4875
                     /gene="nrdE"
                     /locus_tag="ACHUR4_002102"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1455689.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4885..5844
                     /gene="nrdF"
                     /locus_tag="ACHUR4_002103"
     CDS             4885..5844
                     /gene="nrdF"
                     /locus_tag="ACHUR4_002103"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1455690.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6199..7401
                     /gene="proV"
                     /locus_tag="ACHUR4_002104"
     CDS             6199..7401
                     /gene="proV"
                     /locus_tag="ACHUR4_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1455691.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7394..8458
                     /gene="proW"
                     /locus_tag="ACHUR4_002105"
     CDS             7394..8458
                     /gene="proW"
                     /locus_tag="ACHUR4_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1455692.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8516..9508
                     /gene="proX"
                     /locus_tag="ACHUR4_002106"
     CDS             8516..9508
                     /gene="proX"
                     /locus_tag="ACHUR4_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1455693.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9700..10879
                     /locus_tag="ACHUR4_002107"
                     /pseudo
     CDS             9700..10879
                     /locus_tag="ACHUR4_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11003..11740
                     /gene="ygaZ"
                     /locus_tag="ACHUR4_002108"
     CDS             11003..11740
                     /gene="ygaZ"
                     /locus_tag="ACHUR4_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1455694.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11730..12065
                     /gene="ygaH"
                     /locus_tag="ACHUR4_002109"
     CDS             11730..12065
                     /gene="ygaH"
                     /locus_tag="ACHUR4_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1455695.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12156..12686
                     /gene="emrR"
                     /locus_tag="ACHUR4_002110"
     CDS             12156..12686
                     /gene="emrR"
                     /locus_tag="ACHUR4_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1455696.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12813..13985
                     /gene="emrA"
                     /locus_tag="ACHUR4_002111"
                     /pseudo
     CDS             12813..13985
                     /gene="emrA"
                     /locus_tag="ACHUR4_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            14002..15540
                     /gene="emrB"
                     /locus_tag="ACHUR4_002112"
     CDS             14002..15540
                     /gene="emrB"
                     /locus_tag="ACHUR4_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1455697.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15604..16119)
                     /gene="luxS"
                     /locus_tag="ACHUR4_002113"
     CDS             complement(15604..16119)
                     /gene="luxS"
                     /locus_tag="ACHUR4_002113"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1455698.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16269..17825)
                     /gene="gshA"
                     /locus_tag="ACHUR4_002114"
     CDS             complement(16269..17825)
                     /gene="gshA"
                     /locus_tag="ACHUR4_002114"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1455699.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17898..18326)
                     /locus_tag="ACHUR4_002115"
     CDS             complement(17898..18326)
                     /locus_tag="ACHUR4_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1455700.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18323..18889)
                     /gene="yqaB"
                     /locus_tag="ACHUR4_002116"
     CDS             complement(18323..18889)
                     /gene="yqaB"
                     /locus_tag="ACHUR4_002116"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1455701.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4584 a         4921 c         4803 g         4803 t
ORIGIN      
        1 gttattttct gaaactctca gaataatttc aagattatat attagccatt ttgatgagta
       61 atttaggtta ttggtgaaca gagtggcgct ctctgctcgc taactccgca agcctgacaa
      121 aaccgaagtg aataaaaatg caatataaca gaataaaaga aaacacccct cactggtgct
      181 ttctgctgtt aatgtacatt ttctggcatc aaaaatgctg taaatgaccc ggtcatgaca
      241 aacaccgaat tctctacgct tatagcgtta cctccccccc aaactttcat tagtgaaatt
      301 aaaggcaaga tcatatctaa ttgatttaat tgaaaataaa tcatattggc atgtaaaaat
      361 ccacaatgta caaaaaacga gccgttacgg aatattttat ctacaaaaac taactaaata
      421 aaaatttttc actaattgat tagtcataga cagcgatata cgctatgcaa aaatgcagat
      481 ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt attaaggagc
      541 attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac gttcgcgatg
      601 gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg aatgagcttc
      661 gaacagtctt tttattccag attggtagcg attccggtga acatcttaat tgcatggcca
      721 tacggtatgt accgcgatct gtttatgcgc gcggcacgca aagttagccc gtcgggctgg
      781 ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt gtatgtggcg
      841 atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag ttcaaacatc
      901 gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg ccgccgactg
      961 tttaaagtca gccgttacca gcaggtaaaa gcctgactct tcttttcgcg actggcatcg
     1021 ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt caagcgcaac
     1081 acgcgaacta aagccatgcg ggataccgta atgctcgtgt cgctcgcctg ctaaatagag
     1141 cggaaattgc tggtaatgat cgcaagtttt aatatcggaa gcgggttcag ccagtgttga
     1201 actgcgttct tttaatgttg gatgaattac cagagcggta cgtcccattc gcgcttcgcg
     1261 attaacatag acataatttt ccccacggcg gtaaccatac gctttctggg ttaccacatc
     1321 gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta aatacatatt
     1381 tgatcctcgt tattttcaac cgcgggccta ccttacctga ttgcgcattc acaacgcttt
     1441 cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc tacactgaaa
     1501 attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca acgacgttga
     1561 tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagcc tcgaatctgt
     1621 attgaagtcc tggggcagcg acgccaaagg ggaagctgaa gccgcacgca gtaaagctca
     1681 ggcgttgctg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc agcaagccgc
     1741 gcgcgatgcc gttggctgag cggattcttt tgttcgtgaa agaccctggt gtagcgtggg
     1801 tacagcagct gcggtaggta tttttatcgg cgcactgtta agcatgcgca aatcgtagtg
     1861 caaaaatgat aataaatacg cgtctttgac cccgaagcct gtcttcgggg tttctttttg
     1921 cctggtgaat cacaaaaatc cccctccccc gtcacgctca tatccagggt aatttcgatc
     1981 actatttgct atatattgtg tagttgaatc ttttttcaac tacatctagt atctctgtat
     2041 caacagagag acaacccgac gcgtatcatc gcgccgtatc ttcattttaa acggaaatac
     2101 gaatcatgcg cataactatt tatactcgta acgattgcgt tcagtgccac gccaccaaac
     2161 gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc gttcctgaag
     2221 cagcggaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt gctggcgatc
     2281 ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg ccacacgcgg
     2341 ccagtgcatg agccagctcg tctacttctc cagcagctcc gaaaacacgc agcgttttat
     2401 cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac ggattcaggt
     2461 agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt
     2521 gccacgacag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt
     2581 tattgcttct ggtaatcgca actttggtga ggcgtatggc cgcgccggag atgtgattgc
     2641 ccggaaatgc ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc aaagcgatat
     2701 cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga atgcctgacg
     2761 caggaaacga tggattacca cgcgctgaat gcgatgctta atctttacga tagcgcaggt
     2821 cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttattgcgac gcatgtgcgt
     2881 ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt caacgaaggt
     2941 tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac gctgtttgcc
     3001 cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa gttttacacc
     3061 agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc cgatcgagtc
     3121 acgatggtgg cgctgacgct ggcacaaggc gatgagacgc tggcgttgca actgaccgat
     3181 gaaatgctgt caggacgctt tcagccagcc acgccaacat tcctcaactg cggtaagcag
     3241 cagcgcggcg aactggtttc ctgttttttg ctgcgtattg aagacaatat ggagtcgatt
     3301 ggtcgggcgg taaattccgc actgcagctg tcgaaacgcg gcggcggcgt agcatttttg
     3361 ctgtcgaatc tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca atcttctggc
     3421 gtaattccgg tgatgaaaat gttggaagac gcattttcct atgccaacca actcggcgcg
     3481 cgtcaggggg caggtgcagt ctatttacat gctcatcatc ccgatattct gcgttttctc
     3541 gacacgaaac gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc gctgggcgtg
     3601 gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc gctgttttcg
     3661 ccctatgacg ttgagcgagt ttatggcaag ccgtttgccg atatcgccat tagcgaacac
     3721 tatgacgaac tggttgccga tgaacgcatc cgcaaaaaat acctcaacgc ccgtgatttc
     3781 ttccagcgac tggcagaaat ccagtttgag tccggctatc cctacatcat gtatgaagac
     3841 acagtaaacc gtgctaaccc tatcgccggg cgcataaata tgagtaatct ctgctcagaa
     3901 attttgcagg ttaacagcgc ctcagagtat gacgagaatc tcgactatgc ccgcacaggc
     3961 catgatattt cctgcaattt aggttcgttg aatattgcgc acaccatgga ttcccccgat
     4021 ttttcccgca cggtagagac tgccgtgcgc ggtttaacgg cggtgtcaga tatgagtcat
     4081 atccgcagcg tgccgtccat cgaagccgga aatgccgcct cgcacgccat cggactgggg
     4141 cagatgaatt tacatggtta tctggcgagg gaaggcatcg cttatggttc gccggaagcc
     4201 ctggatttca ccaatctcta tttctatacc atcacctggc acgcactgcg tacctcgatg
     4261 ttgctggcgc gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg ctatgccagt
     4321 ggtgaatatt tcagccaata tctgcaaggt aactggcagc cgaaaacggc gaaagttggc
     4381 gaactgtttg cccgcagctg tattacgtta cctacccgtg agatgtgggc gcaactgcgc
     4441 gacgacgtga tgcgctacgg catatacaac cagaatctcc aggcggtgcc gccaaccggg
     4501 tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc gaaagtagag
     4561 atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat gactaacgaa
     4621 aatctggcgc tgtatcagga cgcttacgaa attggcgcag aaaagatcat cgacacctac
     4681 gcggaagcga ctcgccatgt cgatcagggg ctgtcgctga cgctgttttt ccccgatacc
     4741 gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg tatcaaaacg
     4801 ctctattaca tccgcctgcg tcagatggcg ctggaaggca ctgaaattga aggctgcgtc
     4861 tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat caactggaac
     4921 aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa tttctggcta
     4981 ccagaaaagg tgccgctgtc gaacgatatt cccgcctggc agacattaac tgtcgtagaa
     5041 caacaactga cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct gcaaaatgtt
     5101 atcggcgcgc cttcgctgat gcccgatgcg ctcacgcctc atgaagaggc ggtattatcg
     5161 aatatcagct ttatggaagc ggttcatgcc cgctcttaca gttcgatttt ctcgacgctg
     5221 tgccagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc accgttgcag
     5281 cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa aaagaaaatc
     5341 gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc gatgtatttt
     5401 tccagccgcg gaaagctgac caataccgcg gacctgatcc gtctgattat ccgcgatgaa
     5461 gcagtccacg gttactacat aggctataaa tatcagaaaa acatggaaaa gatatctctg
     5521 ggacaacgtg aagagttgaa gagtttcgcc ttcgatttgt tgctggaact ctacgacaac
     5581 gagttgcaat acaccgatga gctgtacgcc gaaaccccgt gggctgacga tgtgaaagcg
     5641 tttctctgtt acaacaccaa taaggctttg atgaatctgg gctacgaacc gttatttccc
     5701 gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa tgccgatgaa
     5761 aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc ggttgaaaca
     5821 gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta cccgcagcag
     5881 ggaaataatt cccgccaaat agctttttat cacgcaaata ttctgtagtg atctacactg
     5941 atactctgtt gcattattcg cctgaaacta caatattcag gcgttttttc gctatctatg
     6001 acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacgga aaattccggc
     6061 gatttgctcg catcaatatt catgccacat ttgccctcag gggttgcctc agattctcag
     6121 tatgttaggg tagaaaaaag tgactattcc cattgggtaa tatatcgaca taaacaaata
     6181 aagaaatcat tctattgcat ggccattaaa ttaaaaatta aaaatcttta taaaatattt
     6241 ggtgagcatc cacagcgagc gttcaaatat atcgaacaag gactttcaaa agaacaaatt
     6301 ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat tgaagaaggc
     6361 gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt acgccttctc
     6421 aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga tattgccaaa
     6481 atatccgacg ccgaactccg tgaggtgcgc agaaaaaaga ttgcgatggt cttccagtcc
     6541 tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat ggaattggcc
     6601 ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca ggtcgggctg
     6661 gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca acgtgtggga
     6721 ttagcccgcg cgttagcgat taatccggat atattattaa tggacgaagc cttctcggcg
     6781 ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca ggcgaaacat
     6841 cagcgcacca ttgtctttat ttcccacgat cttgatgaag ccatgcgtat tggcgaccga
     6901 attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga aattctcaat
     6961 aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag tcaggtgttc
     7021 agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac tcctggcttc
     7081 ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg ctacgttatc
     7141 gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac cgcgttaacg
     7201 cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga tgcacaaacg
     7261 cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc cgtggtcgac
     7321 gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc tttagatcgt
     7381 gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag cggcggacag
     7441 tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccggctg acggcggtgg
     7501 tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata ttctcgatcc
     7561 gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg actgggttgt
     7621 tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata tcctcaacgg
     7681 tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt tcgctctcat
     7741 cacctggcag atttccgggg tcggaatggg cgtggcgacg ctggtctcgc tgattgccat
     7801 cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt taactgccct
     7861 gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa gtccgcgagc
     7921 ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt ttgtttatct
     7981 ggtgccaatc gtcatgctat ttggtatcgg taacgtgccg ggcgtggtgg tgacgatcat
     8041 ctttgcgctg ccgccgatta tccgtctgac cattctgggg attaaccagg ttccggcgga
     8101 tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt tcaaagttca
     8161 gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga tgctggccct
     8221 ttctatggtg gtcattgcct cgatgattgc cgtcggcggg ctgggtcaga tggtacttcg
     8281 cggtatcggt cgtctggata tggggcttgc caccgttggc ggcgtcggga ttgtgatcct
     8341 cgccattatc ctcgatcgcc tgacgcaggc cgttgggcgc gactcacgca gtcgcggcaa
     8401 ccgtcgctgg tacaccactg gccctgttgg tctgctgacc cgcccattca ttaagtaact
     8461 ctgcacttgc ccggtgacgc cgggcattat caccctgcca aaaaaaggaa taacaatgcg
     8521 acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa cttttgctgc
     8581 cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca ctgaagaaac
     8641 cttccagacg ctgctggtca gtcgtgcgct ggagaaatta ggttataccg tcaacaaacc
     8701 cagcgaagtg gattacaacg ttggctacac ctcgcttgct tccggcgatg caaccttcac
     8761 cgccgtgaac tggacgccac tccatgacaa catgtacgaa gctgccggtg gcgataagaa
     8821 attttatcgt gaaggggtat ttgttaacgg cgcagcacag ggttacctga tcgataagaa
     8881 aaccgccgac caatacaaaa tcaccaacat cgcacaactg aaagatccga agatcgccaa
     8941 actgttcgat accaacggcg acggtaaagc ggatttaacc ggttgtaacc ctggctgggg
     9001 ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca ccgtgacgca
     9061 taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca aagagggcaa
     9121 accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga agccagggaa
     9181 agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa atgccgatac
     9241 caaactgccg aatggcgcga attacggctt cccggtcagc accatgcata tcgttgccaa
     9301 caaagcctgg accgagaaaa atccggcggc agccaaactg tttgccatta tgcagttgcc
     9361 agtggccgat attaacgccc agaacgccat tatgcatgac ggcaaagcct cagaaggcga
     9421 tattcagggc cacgttgatg gctggatcaa agcccaccag cagcagttcg atggctgggt
     9481 gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataaggc gttttcgcct
     9541 catccgccgc tctgtacaca atgcctgatg cgacgctgac gcgtcctatc atgcctacaa
     9601 accacatcgc acatttcaac aagctatctt tcatcccata ttcatcaaca tccgctatta
     9661 ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc taatcatgag
     9721 cttagcccgg cgctgatcgt gctgatgtct atcgccaccg gtctggcggt agccagtaac
     9781 tattacgccc agccattgct cgacaccatc gcgcgtaact tttccctttc cgccagttcg
     9841 gcaggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctact gtttcttgtt
     9901 cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcagcg
     9961 ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatgatcct cggtacggca
    10021 ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct
    10081 tcaccggata aacgcggcaa agtggttggc accattatga gcggtctgct gttggggata
    10141 ttgctggcac gaaccgttgc cgggttactg gcaaacctcg gcggctggcg caccgtcttt
    10201 tgggttgctt cggtgttaat ggcactgatg gcgctggcat tatggcgtgg tctgccacaa
    10261 atgaaatcag aaacccacct caactaccca cagttgttgg gttccgtttt cagcatgttc
    10321 atcagcgata agatcctgcg cacccgcgcg ttgctgggct gcatgacctt tgccaacttc
    10381 agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat
    10441 ggtgacattg gtctgtttgg gcttgcggga gctgccggag cgttgggcgc tcgtccggcg
    10501 ggcggttttg ccgacaaggg caaatcgcac cacaactttc ggtctgcttc tgctgttact
    10561 ttcatggctg gcaatctggt ttggtcacac ttccgtactg gcgttgatta tcggcattct
    10621 ggtgctggac ctcaccgtac agggcgtgca tatcactaac cagacggtaa tttatcgaat
    10681 acatcctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg
    10741 tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg
    10801 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
    10861 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
    10921 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
    10981 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
    11041 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata ttccggtggc
    11101 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
    11161 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg
    11221 gagtagtttg tgggttgctg cactgagcgt catggcaatg gatgttcgcc atgttttgta
    11281 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctatg
    11341 ggcgtttggc ctgacggatg aggtttttgc cgccgccacc gcaaaactgg tacgcaataa
    11401 tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
    11461 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
    11521 tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
    11581 ccagcgcaaa caatctcttt gcgttaccgc agctttagtt ggtgcccttg caggcgtaac
    11641 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
    11701 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac
    11761 tggttggcgc ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg
    11821 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
    11881 tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg
    11941 tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
    12001 tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta
    12061 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt
    12121 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
    12181 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
    12241 tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat aaaatgctga
    12301 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
    12361 accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt accaacgcga
    12421 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtaaa agcgataacg
    12481 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt
    12541 taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag
    12601 atcagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg
    12661 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa
    12721 agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca
    12781 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
    12841 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
    12901 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcatttcg
    12961 aagaaaccga tgacgcatac gtggcaggga atcagattca aattatgtct caggtgtctg
    13021 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gatgttctgg
    13081 tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt
    13141 ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttgcag gcgaatattg
    13201 aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg
    13261 gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg
    13321 cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg atgattctgg
    13381 ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta cgtaacgcct
    13441 agctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtt tcccgtcgtg
    13501 ccgtgcaacc gggtgcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
    13561 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgcatcggtc
    13621 agccggtcac catcaccacc gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
    13681 ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa aatgcgaccg
    13741 gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat cgaactggac cagaaacagc
    13801 tggagcaata cccgctgcgc attggtttgt ccacgctggt gagcgtcaat accactaacc
    13861 gtgacggtca ggtactggca aataaagtac gttcaacacc ggtcgcggta agtaccgcgc
    13921 gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
    13981 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc
    14041 attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctgaactc caccattgct
    14101 aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca aggaacgtgg
    14161 gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
    14221 aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc tattgcgtct
    14281 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
    14341 attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa ctacccgcca
    14401 gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc
    14461 ggcccgatcc tcggcggtta tatcagcgat aattaccact ggggctggat attcttcatc
    14521 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaaccctgcg cggacgtgaa
    14581 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
    14641 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actgtttttc atcacaggaa
    14701 attatcattc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt ctgggagctg
    14761 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
    14821 tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg ctattgttct gctgccgcag
    14881 ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta
    14941 gggattattc cggtgattct gtcgccgatt atcggccgct tcgcgcataa actggatatg
    15001 cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
    15061 acctttgaac caggtatgga tttttgcgcg tcggcctggc cgcagtttat ccaggggttt
    15121 gcggtggtct gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa
    15181 cgactggcgg cggcatcgag cctctctaac tttacgcgaa cgctggcggg gtctatcggc
    15241 acgtcgataa ccacgaccat gtggaccaac cgcgagtcga tgcaccatgc gcagttgact
    15301 gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt acagtcaact ggaagggctt
    15361 gggatgacgc aacagcaggc atcaggctgg attgcccagc agatcaccaa tcaggggctg
    15421 attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt actgctgggg
    15481 ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa
    15541 gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac
    15601 tgactagatg tgcagttcct gcaacttctc tttcggcagt gccagttctt cgttgctgtt
    15661 gatgcgtacc tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg
    15721 catctggtaa gtgccacact ggtagacgtt cagttccggg atctgattct gatcctgcac
    15781 tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct catctggcgt
    15841 accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc
    15901 tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg
    15961 gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt
    16021 gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat
    16081 ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc cggtaatttt
    16141 tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat
    16201 ttgttatcat catccctgaa ttcagagatg aaattttggc cactcccgag tggccttttt
    16261 cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc agcggtttcc
    16321 atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga
    16381 atttccagcg gctcttcacg cagcagatta cggtaggctt ctgcaaacgc tttgcctgtt
    16441 ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga
    16501 ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaatg
    16561 ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcagt
    16621 aacgggaact gcgcggtttc gcagccgata cccagcgtca ggcccggttt gcgaccttca
    16681 aggatcaccc ggttccagtt aacgcgcgta caggcaagtt cgctactgct catttccggt
    16741 gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct
    16801 acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg
    16861 ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg taacgcgttt tggacgaatc
    16921 ggcgcataca gttcgttttc aatctgcagc acgttgctat tgatttgcag cctcttaccg
    16981 tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttaagg
    17041 cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg
    17101 gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac
    17161 tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga acaaatcgcc
    17221 ggagatgcac caaacagata aggaataacc caaccgaaac gatagtaatt gcggataacg
    17281 cggaaatagc ccgcagaaat tttctctttg gcatcagcgc ccgagatatc accgcacttc
    17341 gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc
    17401 agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg tccggtgtta
    17461 gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt
    17521 aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata
    17581 aaggtcagca tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc
    17641 gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct
    17701 gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt
    17761 atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg
    17821 atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca
    17881 tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtcgc tgctgcaact
    17941 gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttactggccc ccaggagatg
    18001 cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat
    18061 agcgtgactg caccatagcg tttcagccag ccggtagctt tctcttgcca gcgcgatgtt
    18121 ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag gctattaccc
    18181 attgttgctg ttaaaactaa aacccaggga tgactgatcc cggaaagcaa cattgccacc
    18241 agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac
    18301 gagaaaagcg ataacgcttc actcacagca agcgaacatc cacagcgtcc atgcctgctg
    18361 cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca
    18421 cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt
    18481 gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct
    18541 cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt
    18601 tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt cttactgctt
    18661 ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg
    18721 cctgagcaat acgccaggtg ggcgatccat taagcgcaat catcgcctga atatcgtact
    18781 gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc gccgtatcca
    18841 ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc
    18901 aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag
    18961 gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga
    19021 aattttttgg agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    19081 gccgagtact ctatccagct gagctacgga t
//
LOCUS       DAZPFP010000064        19025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPFP010000064 DAZPFP010000000
VERSION     DAZPFP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.064"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHUR4_002117"
     CDS             complement(<1..1025)
                     /locus_tag="ACHUR4_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455702.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHUR4_002118"
     CDS             complement(1127..2107)
                     /locus_tag="ACHUR4_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1455703.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHUR4_002119"
     CDS             complement(2115..2825)
                     /locus_tag="ACHUR4_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1455704.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUR4_002120"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUR4_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1455705.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUR4_002121"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUR4_002121"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1455706.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUR4_002122"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUR4_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1455707.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUR4_002123"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUR4_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1455708.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHUR4_002124"
     CDS             9402..9512
                     /locus_tag="ACHUR4_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1455709.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUR4_002125"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUR4_002125"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1455710.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUR4_002126"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUR4_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1455711.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHUR4_002127"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHUR4_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUR4_002128"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUR4_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1455712.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUR4_002129"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUR4_002129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1455713.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHUR4_002130"
     CDS             16558..17490
                     /locus_tag="ACHUR4_002130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1455714.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHUR4_002131"
     CDS             complement(17531..18961)
                     /locus_tag="ACHUR4_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1455715.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4652 a         4804 c         4557 g         5012 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggccag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagc
//
LOCUS       DAZPFP010000065        18968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPFP010000065 DAZPFP010000000
VERSION     DAZPFP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.065"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHUR4_002132"
     CDS             <1..247
                     /locus_tag="ACHUR4_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1455716.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACHUR4_002133"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACHUR4_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1455717.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACHUR4_002134"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACHUR4_002134"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1455718.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="ACHUR4_002135"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="ACHUR4_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR1455719.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACHUR4_002136"
     CDS             complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACHUR4_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1455720.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACHUR4_002137"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACHUR4_002137"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1455721.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="ACHUR4_002138"
     CDS             complement(4443..5651)
                     /locus_tag="ACHUR4_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455722.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="ACHUR4_002139"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="ACHUR4_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1455723.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="ACHUR4_002140"
     CDS             complement(6446..8131)
                     /locus_tag="ACHUR4_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1455724.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="ACHUR4_002141"
     CDS             8401..8778
                     /locus_tag="ACHUR4_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1455725.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACHUR4_002142"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACHUR4_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1455726.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="ACHUR4_002143"
     CDS             9225..9512
                     /locus_tag="ACHUR4_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1455727.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="ACHUR4_002144"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="ACHUR4_002144"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1455728.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="ACHUR4_002145"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="ACHUR4_002145"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1455729.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="ACHUR4_002146"
     CDS             11269..11745
                     /locus_tag="ACHUR4_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1455730.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="ACHUR4_002147"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="ACHUR4_002147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1455731.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14470
                     /gene="potG"
                     /locus_tag="ACHUR4_002148"
     CDS             13304..14470
                     /gene="potG"
                     /locus_tag="ACHUR4_002148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1455732.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14480..15433
                     /gene="potH"
                     /locus_tag="ACHUR4_002149"
     CDS             14480..15433
                     /gene="potH"
                     /locus_tag="ACHUR4_002149"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1455733.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15430..16275
                     /gene="potI"
                     /locus_tag="ACHUR4_002150"
     CDS             15430..16275
                     /gene="potI"
                     /locus_tag="ACHUR4_002150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1455734.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16335..16823
                     /locus_tag="ACHUR4_002151"
     CDS             16335..16823
                     /locus_tag="ACHUR4_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1455735.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16864..17991
                     /gene="rlmC"
                     /locus_tag="ACHUR4_002152"
     CDS             16864..17991
                     /gene="rlmC"
                     /locus_tag="ACHUR4_002152"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1455736.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="ACHUR4_002153"
     CDS             complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="ACHUR4_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1455737.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         4585 a         4677 c         4963 g         4743 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccctaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgg gcgcatccgg
    13501 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca
    13561 gataatgctt gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat
    13621 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    13681 cctgaaacag gacaaactac cgaaagcgga aatttccagt cgggtcaatg agatgctcgg
    13741 cctggtgcac atgcaggagt tcgccaaacg taaaccgcat cagctttctg gtggtcagcg
    13801 acagcgtgtg gccctggccc gaagccttgc caagcgcccg aaactattac tgctcgacga
    13861 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    13921 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    13981 catggcgggg cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccgga
    14041 agagatctac gagcatccga ctacccgcta tagcgccgaa tttattggct cggtaaacgt
    14101 ctttgaaggc gtactcaaag agcgtcagga agatggcttg gtgcttgatt cgccggggct
    14161 ggtgcatcca ctgaaagtcg acgcggatgc ctcggtggtc gataacgtgc cggtacatgt
    14221 ggcgctgcgc ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt
    14281 cgcggtgggg gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg
    14341 tctgaaaagt gggcagatga ttagtgccca gctacaaaac gcccatcgtc atcgtaaagg
    14401 gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    14461 gacggtttaa ggagcaaaga tgaatacact tgaacctgct gcccagtcga aaccgccggg
    14521 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    14581 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    14641 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    14701 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct
    14761 ctatttcgat gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt
    14821 actgatcggc tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat
    14881 tttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcttg
    14941 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    15001 cgatcaaccg ctgaccattc ttcataccaa tctggcagtt tatattggca ttgtttacgc
    15061 ctacgtgccg tttatggtgc tgccgattta taccgcgttg attcgtattg attattcgct
    15121 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    15181 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    15241 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    15301 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    15361 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    15421 agaacacgga tgaataattt accggtagtt cgttcgccct ggcggattgt gattttgctg
    15481 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    15541 tcgaagctgg tgacggtgtg gggcggctgg tcaacgcgct ggtatggtga gttattgcgc
    15601 gatgacgcga tgatgagtgc ggttggttca agcctgacaa ttgctgcctg tgcggcaacg
    15661 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    15721 ggatcaaatg gctttgcctt tatgatcacc gcgccgctag taatgccaga tgtcatcacg
    15781 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    15841 cgcggtatgc ttaccatctg gctggcgcat gtcactttct gtacggctta tgtggcggtc
    15901 gttatttcgt cgcgtctgcg ggaactggat cgctcgatag aagaagccgc gatggatctc
    15961 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc
    16021 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    16081 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    16141 gtgaatccgg aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga
    16201 tttatcgcct ggtatctgat ggcgcgcgca gaaaaacagc ggatacgcga catccaacgt
    16261 gcaagacgtg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc
    16321 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    16381 atgctcgcct gaatgtgcct gcgctggtgc aggtggcggc gctcgccatt attatgatcc
    16441 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    16501 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    16561 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    16621 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    16681 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    16741 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    16801 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    16861 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    16921 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgatttt
    16981 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaagcc
    17041 aaaatggtgg tgagtggtag cgttgaaaaa acactgctcg gtatgctgca tcgcgatggc
    17101 acgccggaag acctttgtga ctgcccgctt tatccagcct catttgcgcc cgtttttgcg
    17161 gcgctaaaac cgtttatcgc ccgagcgggg ttaacgccct acaacgtggc gcgtaagcgt
    17221 ggcgaactga aatacattct gctgactgaa agccagagtg atggcggcat gatgctgcgc
    17281 tttgtactgc gttctgatac caagctggcg caactgcgta aggcgctgcc gtggttacac
    17341 gaacaactac cgcagctgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    17401 atggaagggg agacggagat ctacctgacc gaacaacagg cactggcgga gcgttttaat
    17461 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga ctaatccggc ggtcgccagc
    17521 cagttgtacg ccaccgcgcg cgactgggta cgacagctgc cggttaaaca tatgtgggat
    17581 ctgttctgcg gtgtgggggg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    17641 gggatcgaaa ttgcgccaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggc
    17701 ttaactcgtt tacaatttca ggcgctggac tccactcagt ttgccactgc tcagggggag
    17761 gtgccggagc tggtgctggt taacccgccg cgccgcggca ttggtaaacc gctgtgtgat
    17821 tatctttcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    17881 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    17941 ttcccgcaca ccgcgcacta tgaagtgttg acgctgctgg tgaagcaata aaaaagccac
    18001 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccgga
    18121 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcggaaacca
    18181 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    18241 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    18301 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    18361 gtttaccacc gcggtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    18421 ggcattctga taactgtcat aggagacagt tttcacttcc gggtgctgat cctgaatata
    18481 tttctggtgc gtcgtaccgt tttccatccc aatgcgtttg cctttcaggt cggcaaacgt
    18541 tttgtaggta tcttttttgg aaatcacgac ggctgagttt tcatagtagg gtgtggtaaa
    18601 cgaaacctgc ttgctacgct ccggggtgat atccataccg gagattacgg cgtcatattt
    18661 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    18721 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    18781 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    18841 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    18901 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    18961 ccatcaag
//
LOCUS       DAZPFP010000066        17926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPFP010000066 DAZPFP010000000
VERSION     DAZPFP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.066"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..1048
                     /locus_tag="ACHUR4_002154"
                     /pseudo
     CDS             1..1048
                     /locus_tag="ACHUR4_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1194..2231)
                     /gene="iap"
                     /locus_tag="ACHUR4_002155"
     CDS             complement(1194..2231)
                     /gene="iap"
                     /locus_tag="ACHUR4_002155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1455738.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2483..3391
                     /gene="cysD"
                     /locus_tag="ACHUR4_002156"
     CDS             2483..3391
                     /gene="cysD"
                     /locus_tag="ACHUR4_002156"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1455739.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3393..4820
                     /gene="cysN"
                     /locus_tag="ACHUR4_002157"
     CDS             3393..4820
                     /gene="cysN"
                     /locus_tag="ACHUR4_002157"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1455740.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4820..5425
                     /gene="cysC"
                     /locus_tag="ACHUR4_002158"
     CDS             4820..5425
                     /gene="cysC"
                     /locus_tag="ACHUR4_002158"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1455741.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5475..5798
                     /locus_tag="ACHUR4_002159"
     CDS             5475..5798
                     /locus_tag="ACHUR4_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1455742.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5992..6303
                     /gene="ftsB"
                     /locus_tag="ACHUR4_002160"
     CDS             5992..6303
                     /gene="ftsB"
                     /locus_tag="ACHUR4_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1455743.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6322..7032
                     /gene="ispD"
                     /locus_tag="ACHUR4_002161"
     CDS             6322..7032
                     /gene="ispD"
                     /locus_tag="ACHUR4_002161"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1455744.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7032..7511
                     /gene="ispF"
                     /locus_tag="ACHUR4_002162"
     CDS             7032..7511
                     /gene="ispF"
                     /locus_tag="ACHUR4_002162"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1455745.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7508..8557
                     /gene="truD"
                     /locus_tag="ACHUR4_002163"
     CDS             7508..8557
                     /gene="truD"
                     /locus_tag="ACHUR4_002163"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1455746.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8538..9299
                     /gene="surE"
                     /locus_tag="ACHUR4_002164"
     CDS             8538..9299
                     /gene="surE"
                     /locus_tag="ACHUR4_002164"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1455747.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9293..9919
                     /gene="pcm"
                     /locus_tag="ACHUR4_002165"
     CDS             9293..9919
                     /gene="pcm"
                     /locus_tag="ACHUR4_002165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1455748.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10104..11198
                     /gene="nlpD"
                     /locus_tag="ACHUR4_002166"
     CDS             10104..11198
                     /gene="nlpD"
                     /locus_tag="ACHUR4_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1455749.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11261..12253
                     /gene="rpoS"
                     /locus_tag="ACHUR4_002167"
     CDS             11261..12253
                     /gene="rpoS"
                     /locus_tag="ACHUR4_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1455750.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12373..12780)
                     /locus_tag="ACHUR4_002168"
     CDS             complement(12373..12780)
                     /locus_tag="ACHUR4_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR1455751.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12927..13520
                     /locus_tag="ACHUR4_002169"
     CDS             12927..13520
                     /locus_tag="ACHUR4_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR1455752.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13520..14467
                     /locus_tag="ACHUR4_002170"
                     /pseudo
     CDS             13520..14467
                     /locus_tag="ACHUR4_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14740..15303)
                     /locus_tag="ACHUR4_002171"
     CDS             complement(14740..15303)
                     /locus_tag="ACHUR4_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455753.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15468..15719
                     /locus_tag="ACHUR4_002172"
     CDS             15468..15719
                     /locus_tag="ACHUR4_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1455754.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15721..17817
                     /locus_tag="ACHUR4_002173"
     CDS             15721..17817
                     /locus_tag="ACHUR4_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1455755.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4422 a         4382 c         4815 g         4307 t
ORIGIN      
        1 aaaaatattg tgactttctt tgatttctgg ggaaaaaacc gacgtggcga gaaagagggt
       61 atcgacgact atcaccttct ttgctggcat ttgctggatg tggccgcaat gggctattta
      121 atggttaaaa gaaattgctt cgggctggct gattattttc gtcaattagg gatttctgac
      181 aaggaacagg cagctcgatt tttcgcctgg ttgctgtgct ggcacgatat tggaaaattt
      241 gcccgctctt ttcaacaact ttacctgccc cctgaattca agattcagga aggtgccaga
      301 aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa gtgaatgtca
      361 ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga tagaaatgtg
      421 gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg aattggataa
      481 ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca agccactgtt
      541 tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac ttataaaaca
      601 actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt cgtcaacgcg
      661 attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa aggctttagt
      721 tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc ctttcaccgg
      781 aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa aggtgctgga
      841 tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga ccggcgcagg
      901 taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta acgctcaggg
      961 gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc gactggtcaa
     1021 aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga taaaccggcg
     1081 caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg atgggtatca
     1141 cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac ccatcaagac
     1201 gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg gcagcgacgt
     1261 tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct tacgtcatgc
     1321 cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc atccttattt
     1381 ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt gtcgaatatt
     1441 tctgcgtcat tacaaccccc agtgcctttc ggataatttt tattcaaacc cggattggtc
     1501 gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt taatttcctt
     1561 actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac aattaagtta
     1621 tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg cttgagtaaa
     1681 ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg aataccatac
     1741 tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa acctgcggcg
     1801 ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc agcatcgctc
     1861 agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc tttgccttca
     1921 tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt atcgcgggcg
     1981 gtataaatat accgactatt aaatgtccga atatcactgc gataacccat ttgctgaaac
     2041 tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat gcgtcccgga
     2101 aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt aggtgacgag
     2161 gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg gtggcgcaat
     2221 gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca tagtatgaaa
     2281 ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg gtgccttaag
     2341 cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact cattctaatt
     2401 ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg caagaaaata
     2461 gcggtattgc aaaggaacgg ttatggctca aaaacgactt actcacctgc ggcaactgga
     2521 ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc cggtgatgct
     2581 ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg cgttttatcc
     2641 aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc gcgagatgta
     2701 tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc
     2761 ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac ataccgatat
     2821 tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg ccgccttcgg
     2881 tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact ctttccgtga
     2941 ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca actacaacgg
     3001 gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga ccgagcagga
     3061 tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga
     3121 acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc gtatcgacct
     3181 gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc
     3241 gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg aagagatgct
     3301 ggtttccacc accagtgaac gtcagggtcg cgtgattgac cgcgaccagg cgggatccat
     3361 ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca caacaaatcg
     3421 ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt
     3481 ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt ctgctgcacg
     3541 atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt aagcgtcacg
     3601 gaacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa gctgagcgcg
     3661 aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag cgtaaattta
     3721 ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact ggcgcatcga
     3781 catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat caaacccgtc
     3841 gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa
     3901 tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat tatctgacct
     3961 ttgccgggca gctgccgggt aatctggata tccgctttgt gccactctcc gcactggaag
     4021 gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg acactgctcg
     4081 aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg cgcttcccgg
     4141 tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg ctggcatccg
     4201 gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg
     4261 cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa gcgatcaccc
     4321 tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg gcagacgaag
     4381 cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt
     4441 ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg
     4501 gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa aacctgccgc
     4561 tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta gatcgctatc
     4621 agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat gtgaccgtgg
     4681 gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct gaattcagtg
     4741 cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg ggcgcgcgcg
     4801 atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc atagtcatcc
     4861 ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc tgtggtttac
     4921 cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg cgttacataa
     4981 actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat tatgcagcga
     5041 tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa
     5101 tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac accgcgccga
     5161 acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt ttgtcgatac
     5221 gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg
     5281 tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat cggcagaaat
     5341 tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag atctgttgag
     5401 acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg ttcgtcaaag
     5461 taacaggatc aaatatgcgt aatagccata acattacact aacaaataac gacagcctta
     5521 ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt atctcctggt
     5581 tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc ttctttctct
     5641 acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg attgcgctgc
     5701 attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg acagtaggca
     5761 ttatgtttgg cgcactgttt atgtggctac tgggctaatt tgtactttct tgtctcctct
     5821 gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct gggtatacgt
     5881 aacaatgggc aaatgtggta catttgcccg cgttgtcgcg gtatccccaa cagaggatgt
     5941 agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag gatgggtaaa
     6001 ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg gttcggtaag
     6061 aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca agctacaaac
     6121 gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct caatggcggc
     6181 caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact
     6241 ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga
     6301 taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc cgtggttccg
     6361 gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt
     6421 aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgacacgt
     6481 gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat
     6541 ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg
     6601 aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat
     6661 caggatgacc ttgcgcgatt gttggcgttg agcgaaacca gccgcacggg agggatcctc
     6721 gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat
     6781 accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg
     6841 ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga cgaagcctcg
     6901 gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga taacattaaa
     6961 gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag
     7021 gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc
     7081 caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg
     7141 gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata
     7201 tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc cgcgagctgc
     7261 tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca
     7321 ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg
     7381 ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac
     7441 ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg
     7501 caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc aaggcaccgg
     7561 gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct ttgagcctga
     7621 tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata cccgttttgt
     7681 ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct tcgctgggca
     7741 aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg gcaaggaaat
     7801 gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg cgcggcacaa
     7861 gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc tgcgcgaagt
     7921 gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa
     7981 ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg
     8041 ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt tgtcggcagc
     8101 ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag acgttaatca
     8161 agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg tagcaaccac
     8221 cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga taaccgccgc
     8281 attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg aacaagcagc
     8341 tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg aagccgcgcg
     8401 cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg acgtcaccgt
     8461 agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca gggaacttat
     8521 caacacaaca ggtgattatg cgcatattgc tgagtaatta tgacggggta catgcacccg
     8581 gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg
     8641 atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg cgcacgttta
     8701 cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt gtctatcttg
     8761 gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt aacgccgggc
     8821 cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg gaaggccgtc
     8881 atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac gacactgccg
     8941 cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta
     9001 ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt cgcgtgacgc
     9061 gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg cgcggcaata
     9121 cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg accgattttg
     9181 ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta actgcgcata
     9241 gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg caatggtaag
     9301 cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt
     9361 gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa
     9421 agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc agccgtatat
     9481 ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc tggaaattgg
     9541 cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg tttgctcggt
     9601 tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc ttgatttaca
     9661 taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg cgccgtttga
     9721 cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga cgcagctgga
     9781 cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga aacgggtgcg
     9841 tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg tccctttagt
     9901 gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt tattttgccg
     9961 taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg cacggtaatt
    10021 atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga agcccaaaat
    10081 tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca ggctgttctg
    10141 acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct gcaaatacta
    10201 attctggtat gttgattacg ccgccgccga aaatggggac gacgtctaca gcgcagcaac
    10261 cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg caaatccagc
    10321 cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc gtctataacc
    10381 gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc gtgaaaaaag
    10441 gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac cttgctcagc
    10501 gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag gtgggtaatg
    10561 cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca gcagagcaag
    10621 gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta
    10681 cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac aacaagccaa
    10741 ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc gagccgactg
    10801 tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact gagggcaaag
    10861 tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc gcaggcagca
    10921 aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt aacgcgctgc
    10981 gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt gcctacgccc
    11041 ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa
    11101 ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt tacaagggga
    11161 aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc aggcttttgc
    11221 ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga atacgctgaa
    11281 agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg tttttgacga
    11341 aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg aactgttatc
    11401 gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta
    11461 ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga
    11521 tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa aaattgcccg
    11581 ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca acttggggct
    11641 gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa catacgcaac
    11701 ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta ctattcgttt
    11761 gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca
    11821 taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga
    11881 tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg
    11941 tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga acggtccgga
    12001 agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt tcgagctgaa
    12061 cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg aagcggcaac
    12121 actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc agattcaggt
    12181 tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct
    12241 gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttt
    12301 cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc gtcctacccg
    12361 gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca gctcctgctc
    12421 ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag gtatcgccgc
    12481 agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct tgtcagcgac
    12541 gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt ctgctagcgt
    12601 tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag gtttatcagc
    12661 aatggcgcgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt gctgccagcg
    12721 agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc gtaacgccat
    12781 gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac gaagaagatg
    12841 ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata ataacgttcg
    12901 tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca ggggctaccg
    12961 gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat gtcgagactc
    13021 atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct tacagcgctc
    13081 gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg gcgatcatct
    13141 catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg aaaacgctcg
    13201 ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac gtcgtgctca
    13261 aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg
    13321 aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg cctgcctttt
    13381 ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc gtgctggatc
    13441 aatttggcct cgaacatccc cacgccaggc gctggcaagg attgccgcag gcccggaatt
    13501 tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac aggcgcttga
    13561 tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg atctggcagc
    13621 agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt ttgataatat
    13681 tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc agaaccacgc
    13741 gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg agtttatccg
    13801 ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa
    13861 caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc gcctgaatga
    13921 tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc cgctcgaccc
    13981 ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg gcaagcgtaa
    14041 gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata aagcagaaga
    14101 gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca tcacgctgat
    14161 gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg cgctgcgtga
    14221 aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt ggggttcaga
    14281 agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc cgttcggtga
    14341 gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg ataaagtctc
    14401 ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga ccgaaatcga
    14461 ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa agacgggaat
    14521 agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt ccaaaaatgt
    14581 taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac aatagccttt
    14641 aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc aagcgccaaa
    14701 accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg ctgctttctg
    14761 aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc taaccttttc
    14821 gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta gagcgtgcaa
    14881 ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc tcccaagaac
    14941 atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta gaatttgagc
    15001 atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt caacatcacc
    15061 ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct tttttaactg
    15121 atacgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta aagtgccttc
    15181 attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta ctaacctaaa
    15241 gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg gtgcttttct
    15301 cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa aataaaccat
    15361 aaaaacacca aagaaccctt tgcagggaat ctttggtatc ttaatcttta tccatacgga
    15421 tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg agacaaagag
    15481 aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa gataacgggt
    15541 ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca ctgaaagcgg
    15601 tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata ggtgtaccgc
    15661 cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag gcgtcgtgat
    15721 atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc agccaccaca
    15781 aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt tagtcgccgt
    15841 cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat aacacgaaag
    15901 gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt ttctgaagtg
    15961 aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga agcatatcgc
    16021 ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca gcgctgggta
    16081 tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc cggtaacccc
    16141 gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga gctgccggag
    16201 cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg tactatcagc
    16261 cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc cgccgaacgg
    16321 ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat cagctggctg
    16381 cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga agcctacgag
    16441 gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct ggaggtgatg
    16501 ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt gaaacaaaaa
    16561 gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg cgactggaca
    16621 gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct gaaatgtgcg
    16681 catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga tggtggaaca
    16741 cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt tgccggtgcg
    16801 atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga taacggttcc
    16861 ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg cctggggatt
    16921 aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga acgcctgaac
    16981 cggacactgc cgatgcgtat agcgaggagg tatcgcacct attttggcaa gggtgctgac
    17041 cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc cctgcaacag
    17101 gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc gtgggcggag
    17161 cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca ttctgagctg
    17221 ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa acgccaggca
    17281 gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat gttccgccca
    17341 atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat ttactacgcg
    17401 ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat tcatgacgcc
    17461 gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat atggaacggc
    17521 aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca gcgtattaaa
    17581 ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg tattcagatt
    17641 ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt tggtaatgtg
    17701 gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga acgcgatttc
    17761 cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct ggcctgaata
    17821 aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat ttgaaataac
    17881 cgaggaaata ctatgactga tattgatgat gtttgaggtg tactgg
//
LOCUS       DAZPFP010000067        17778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPFP010000067 DAZPFP010000000
VERSION     DAZPFP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.067"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            174..857
                     /locus_tag="ACHUR4_002174"
     CDS             174..857
                     /locus_tag="ACHUR4_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1455756.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            901..5563
                     /locus_tag="ACHUR4_002175"
                     /pseudo
     CDS             901..5563
                     /locus_tag="ACHUR4_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5931..7586
                     /gene="lldP"
                     /locus_tag="ACHUR4_002176"
     CDS             5931..7586
                     /gene="lldP"
                     /locus_tag="ACHUR4_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1455757.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7586..8362
                     /gene="lldR"
                     /locus_tag="ACHUR4_002177"
     CDS             7586..8362
                     /gene="lldR"
                     /locus_tag="ACHUR4_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1455758.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8359..9549
                     /gene="lldD"
                     /locus_tag="ACHUR4_002178"
     CDS             8359..9549
                     /gene="lldD"
                     /locus_tag="ACHUR4_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1455759.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9745..10218
                     /gene="trmL"
                     /locus_tag="ACHUR4_002179"
     CDS             9745..10218
                     /gene="trmL"
                     /locus_tag="ACHUR4_002179"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1455760.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10271..11092)
                     /gene="cysE"
                     /locus_tag="ACHUR4_002180"
     CDS             complement(10271..11092)
                     /gene="cysE"
                     /locus_tag="ACHUR4_002180"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1455761.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11172..12191)
                     /gene="gpsA"
                     /locus_tag="ACHUR4_002181"
     CDS             complement(11172..12191)
                     /gene="gpsA"
                     /locus_tag="ACHUR4_002181"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1455762.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12191..12658)
                     /gene="secB"
                     /locus_tag="ACHUR4_002182"
     CDS             complement(12191..12658)
                     /gene="secB"
                     /locus_tag="ACHUR4_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1455763.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12721..12972)
                     /gene="grxC"
                     /locus_tag="ACHUR4_002183"
     CDS             complement(12721..12972)
                     /gene="grxC"
                     /locus_tag="ACHUR4_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1455764.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13114..13545)
                     /locus_tag="ACHUR4_002184"
     CDS             complement(13114..13545)
                     /locus_tag="ACHUR4_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1455765.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13790..15334
                     /gene="gpmM"
                     /locus_tag="ACHUR4_002185"
                     /gene_synonym="pgmI"
     CDS             13790..15334
                     /gene="gpmM"
                     /locus_tag="ACHUR4_002185"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1455766.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15368..16627
                     /gene="envC"
                     /locus_tag="ACHUR4_002186"
     CDS             15368..16627
                     /gene="envC"
                     /locus_tag="ACHUR4_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1455767.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16631..17590
                     /locus_tag="ACHUR4_002187"
     CDS             16631..17590
                     /locus_tag="ACHUR4_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1455768.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17577..17778)
                     /locus_tag="ACHUR4_002188"
                     /pseudo
     CDS             complement(17577..17778)
                     /locus_tag="ACHUR4_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4452 a         4619 c         4647 g         4060 t
ORIGIN      
        1 tacaaacaca gacatctttt gaaacgaatg atacattttt atgatgaatg cttaaaacaa
       61 tatttaatcg acacacatca ctgtcattaa tgtaaagcaa catagctcac caataataca
      121 atggacaact ctaccggcat tgagcgctgg tcggaggaaa taaggatttt tacatgaact
      181 tgaagaagat ttttttcagt gctgtcactg tttcagttct ctgcgcatta acgggatgtg
      241 actacattga agaaggcaag ccagaaagta gtttactgaa acaacaagaa gaacataata
      301 ataaaattgt gctacttgaa aaacaacaag cacagctaaa gagtcaactc gaaactattc
      361 aaaaacaaca aactggcata ataaacagta caaagacttt aactcacgta attaaatccg
      421 ttaaagacca acaaaatact tttattttta ccgagtttaa cccagcaaaa acgaagtatt
      481 tcatcctgaa caacggttcg gtggctttag cgggtcgagt gttatccatt gacgccacag
      541 agaatggtag tgttattcat atctcactgg tcaacttatt aagtacacct atctcaaaca
      601 ttggttttaa tgcgacatgg ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt
      661 ggcaacaatt acttttcaac acatcaatga aatccacatt gaaattatta ccaggtcaat
      721 ggcaagacat taatttgacc ctgaagggtg tatcgcccaa taacctggga tatctgaagt
      781 tagccatcaa catggaaaat attcagttcg acaatcttcc ctctgctgaa aatcggcaga
      841 aaagaagcaa aaaataaatg cagttagtac gcttataaca aggaatagcg agcatcatta
      901 atgaacaaaa tatttaaagt tatctggaac cctgcgacag ggaattatac tgttaccagc
      961 gaaacggcaa aaagccgtgg caagaaatct gggcgcagta agctgttaat ttctgcgctg
     1021 gttgcgggtg gaatgttgtc gtcgtttggg gcattggcga atgccgggaa tgacaacggt
     1081 cagggtgttg attacggtag tggatcagct ggcgacggct gggttgctat aggcaaaggg
     1141 gcgaaagcaa atacttttat gaacaccagt ggttccagta ctgctgtggg ttatgacgct
     1201 atagctgaag gccaatatag ctctgccatc gggtcaaaaa cccatgcgat tggtggtgca
     1261 tcaatggcct ttggggttag tgcaatatca gaaggcgata gaagtatagc actgggtgcc
     1321 tcttcgtatt cattgggcca atactcaatg gccctcggcc gttattcaaa agcattgggt
     1381 aaattgtcta ttgctatggg ggactcttcc aaagcggaag gagcaaacgc cattgccctg
     1441 gggcgtagca gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc
     1501 agcgcagcga acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct
     1561 attggcaata atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt
     1621 gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca
     1681 atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt
     1741 agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat
     1801 aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc
     1861 gcagattcca ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc
     1921 aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt
     1981 tcacagcttt atgccattag cgactcggta gcaaaaaagg cttggaggag gggctgcagt
     2041 agatgttgat gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa
     2101 taacgtaggg gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa
     2161 aggcaaatac agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt
     2221 tgcggatggc acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc
     2281 taccaatgac gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg
     2341 tgaccttcag gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca
     2401 cggccaggat accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag
     2461 cactgatgct gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac
     2521 caccaatatc gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct
     2581 gactgagacg gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt
     2641 attcacggca gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga
     2701 cctgacgact ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc
     2761 cgtggcgacg aataccacca atatcgccac taacaccacc aacatctcta atctgactga
     2821 gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac
     2881 tgcagctcat ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac
     2941 tgcaaccagt tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc
     3001 cacctacttc ggcggcaatg cttctgtgaa tactgacggt gtgtttaccg gtccaaccta
     3061 caaaatcggt gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc
     3121 atttagcacg tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc
     3181 caaacacggt actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc
     3241 agactccagt tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt
     3301 tgatgcgctg gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta
     3361 caccattgct aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac
     3421 tcctgacgac gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc
     3481 tcacggaaaa gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc
     3541 tgccagcagc gacgcgatta acggctcaca actctatacc accaataagt acatcgctga
     3601 tgcgctgggt ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac
     3661 cattgcgaac gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc
     3721 cctgctgtgg gataagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg
     3781 taaagccagc atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc
     3841 agtgaacggt tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat
     3901 caatcagctc gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa
     3961 ctacgttcgt actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg
     4021 cgctacagct gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca
     4081 gaacagcagc agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg
     4141 tgtgattgcc aaaggttctc gtgacactag cgtaacggaa aatggcgtgg tgattggtta
     4201 tgacaccact gacggcgaac tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg
     4261 ccaaatcatc aacgtagctg atggttcaga agcccatgac gccgttacgg ttcgccagtt
     4321 gcagaacgct attggagcgg tcgccactac gccaaccaag tactttcacg ccaactcaac
     4381 ggcagaagac tcactggccg ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt
     4441 taatggtaat gcaggtattg gtatcggttt gaacactctg gttctggctg atgcgatcaa
     4501 cggcattgct atcggcagta acgcacgcgc aaaccatgca aacagtatcg caatgggtaa
     4561 cggttctcag accacccgtg gtgcacagac tggctacacc gcctacaaca tggacgcacc
     4621 gcagaactct gtaggtgagt tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa
     4681 cgtcgcagct ggttcagcgg ataccgatgc ggttaacgtg ggtcagttga aagtcactga
     4741 tgagcgcgta gcgcaaaata cccagagcat tactaacctg aacaatcagg tcactaatct
     4801 ggatactcgc gttactaata tcgaaaacgg tattggcgac attgtcacca ccggtagcac
     4861 caagtacttc aagaccaaca ccgatggcgt agatgccaac gcccagggta aagatagcgt
     4921 tgctattggt tctggttcca ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc
     4981 cgttgcagag gaagaaaata caatctctgt aggttcttct actaaccaac gccgtatcac
     5041 caacgtagct gccagtgtta atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc
     5101 tgaagcaggc ggagtgcgct acgacaccaa agctgatggt tctatcgact atagcaatat
     5161 caccctcggt ggtggcaacg gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa
     5221 caacaacgac gcggtgaact acgcgcagtt gaagcaaagc gcgcaggaaa cgaagcaata
     5281 caccgatcag cggatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag
     5341 cggtggtatc gcttctgcaa tggcaatgac cggtctgccg caggcttata caccgggtgc
     5401 cagcatggct tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt
     5461 atcgatggtg agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca
     5521 gggtgaatac tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt
     5581 gtgactaagg gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg
     5641 ctccgggtca acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac
     5701 atctcccccc acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     5761 ttccccaatt aaaactcaca tcaatattgc caatacataa catttagtta accattcatt
     5821 gtcattatcc ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag
     5881 atgagcaaca gactcattac acgatgtgcg tggactctag gagacctgca atgaatctct
     5941 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc
     6001 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct
     6061 catggacggt agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     6121 acgcgctggc ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca
     6181 ttattgcagc agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     6241 gctcgtctat tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct
     6301 gtttcggcgc tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg
     6361 cattgctggt cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta
     6421 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa
     6481 caggcatcga cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca
     6541 ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc aaagagacgt
     6601 ggcctgcggt cgtggttgca ggcggctcgt ttgccatcgc tcagtacctt agctctaact
     6661 tcattgggcc ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc
     6721 tgttcctcaa acgctggcaa ccagttcgcg tattccgctt cggtgacttg ggggcgtcac
     6781 aggttgatat gacgctggcc cacaccggtt acaccgcggg tcaggtgtta cgtgcctgga
     6841 caccgttcct gttcctgacc gctaccgtaa cactgtggag catcccgccg tttaaagccc
     6901 tgttcgcatc gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctca
     6961 ataaactggt tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt
     7021 ttaagtttga ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga
     7081 ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag
     7141 aactggctct acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact
     7201 attccggact gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct
     7261 tcttctcgcc gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta
     7321 acgccctgtt cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt
     7381 tgctggttgc cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     7441 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     7501 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt
     7561 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     7621 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     7681 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct
     7741 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     7801 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     7861 cgatgatccg gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac
     7921 cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt
     7981 tgaagcaacg ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     8041 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga
     8101 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     8161 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga
     8221 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     8281 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca
     8341 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     8401 cgcattctgc cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg
     8461 ctgcgccgca acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac
     8521 atgtccgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca
     8581 ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa
     8641 gcggcggacg cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa
     8701 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     8761 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     8821 accgtggata tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc
     8881 ccaaatgcgg caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat
     8941 gtgggcctga acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg
     9001 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     9061 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     9121 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac
     9181 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca
     9241 gatgcggtga aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat
     9301 gtcgtgcgta tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat
     9361 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag
     9421 atgaaagtgg cgatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg
     9481 ctggtgcagg ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat
     9541 gcggcatagt cgtttgcgcc ccctcaccct aaccctctcc ctcagggaga ggggaccgtt
     9601 cggcgctgta tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc
     9661 cctccgggga gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc
     9721 ctgcccccgc actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg
     9781 ccaaatactg gcaacatcat ccgtctttgc gctaataccg gctttcgtct acatatcatc
     9841 gaaccgatgg gatttgcctg ggacgacaag cgcctgcgcc gcgcggggct ggactatcac
     9901 gagtttaccg ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc
     9961 cagcgcctgt ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag
    10021 gatggcgatt atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat
    10081 gccctgcccg ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat
    10141 ctgtccaatg cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga
    10201 gcgttgttga gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga
    10261 tcacaggaca ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct
    10321 ggtccatatc cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc
    10381 cagcggcggt tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg
    10441 cgccgcaacc aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt
    10501 cacgaatttt cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt
    10561 gcagaatcga tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt
    10621 ggtcgagcat aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca
    10681 cagaaacctg gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt
    10741 gaccgatgcg ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt
    10801 atttatcgac tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga
    10861 tcatttccgg gtcagcggcg taggcttctt caaccacttc acggatagca atagcaggca
    10921 taattggcga tgacagcttg ttcgccagca tgtagctcag tgcactgcca aggttttcgt
    10981 gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc
    11041 tggcttcggc tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact
    11101 ccacacgatg agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag
    11161 gttccctggg gttagtgact gctgcgctcg tccttgcgtg cacgacctaa taatgtcaat
    11221 gctgcctcgc gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc
    11281 atttcaacgc cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc
    11341 accacctgac caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcata
    11401 ccaaaacggc ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc
    11461 gccatgccca taaaggtggc aggatcggca cccagcgccg caccaagacg cgacatttca
    11521 gccagcccac gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcagac
    11581 atccccgcgc caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa
    11641 tccgggttgc tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg
    11701 gcaaaggtct gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct
    11761 ttcgcaaacg ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc
    11821 gccacgtcct gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga
    11881 cgcgcatcag gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg
    11941 ggtacgacga cgagaatatt acgactggct gccagcgcag tggcgagatc gctttcaaga
    12001 tggagcgtat cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca
    12061 agcgttgcga tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc
    12121 agggtaatgg caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta
    12181 cgttggttca tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat
    12241 agttcatgaa cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc
    12301 gggataccat gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg
    12361 cgcccaggca atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct
    12421 gctgaacttc acacaggaac gcggtttctt cgcccaagga ggccgttacg gtaacacgca
    12481 gtaccacttc gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa
    12541 cttctggttg ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat
    12601 ccttggtata aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt
    12661 gtagaaaacc cttaagtgtt gtccttaaat actgcgcaat gtaataccaa cgttcacacg
    12721 ttatttcagc aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc
    12781 gccaatgtgc tgtgcgtcaa taaaaatctg gggaaccgtg gtgcaaccgc tgcgtttgat
    12841 catctcttca cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt
    12901 gctgctcagc agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc
    12961 aacattggcc ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc
    13021 tgcaaaatgg agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc
    13081 taacggcctg catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt
    13141 ttcgccagcc cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt
    13201 cgtcagcgcg tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccga
    13261 tttgtctttg tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag
    13321 attgatagaa cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac
    13381 cacagcgtct tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt
    13441 cgaggtcagg ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat
    13501 actcagtatg ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc
    13561 ccgacgtagt gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag
    13621 ccagcgcgtt aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg
    13681 catcggcttg ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag
    13741 ctgtagtaaa attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc
    13801 taaaaaacct atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga
    13861 taacgccatt tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca
    13921 taccctgatc gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc
    13981 cgaagtaggc cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct
    14041 ggacgttgaa atcaaagatc gcgctttctt tgctaatcca gtgctggctg gcgcggtaca
    14101 taaagcgaaa aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt
    14161 acacagccac gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga
    14221 aaaaatctac ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc
    14281 ctcgctgaaa aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat
    14341 cattggtcgt tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta
    14401 tgacctgctg actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca
    14461 ggcagcttat gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga
    14521 aggtcagcca gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc
    14581 tgaccgcgcg cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg
    14641 taagaaagtg gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac
    14701 tgcggttgct tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa
    14761 cgacaaaact cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt
    14821 caacggtggc gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa
    14881 agtggctacc tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt
    14941 tgcggccatc aagagcggca aatacgacac cattatctgt aactatccga acggcgacat
    15001 ggtaggtcac accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg
    15061 cgtggaagaa gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga
    15121 ccacggtaac gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac
    15181 caacctgcca gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg
    15241 caaactttct gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga
    15301 gatgactggt aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat
    15361 taataccatg acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc
    15421 cagcgttctt agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga
    15481 ccaactcaaa tctattcagg ccgatatcgc cgcaaaagag cgcgcggtac gccaaaagca
    15541 acaacaacgc gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc
    15601 cacccgtaag ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga
    15661 gatgaacgcg tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc
    15721 cgcacaactg gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag
    15781 cggtgaagaa agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc
    15841 gcgacaagaa accattgccc agttgaagca aacgcgtgaa gaagtcacca tgcagcatgc
    15901 tgaactggaa gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca
    15961 ggcgaaactg actcaggctc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc
    16021 catccagcaa ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa
    16081 cagcattgcc cgtgctgaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc
    16141 ccaggcggtt cgcgagcgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac
    16201 cgaaagcgaa aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc
    16261 attctggccg gttcgcgggc cgacgctgca tcgctatggc gagcagctac agggtgaatt
    16321 acgctggaaa gggatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga
    16381 cggtcgagtg attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca
    16441 cggtaaaggc gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc
    16501 gcaggttcgc gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc
    16561 ttcactctat ttcgaaattc gccgccaggg tcaggcggtc aatccacagc cgtggttggg
    16621 aagataagtt ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct
    16681 ctcctccccc gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc
    16741 gcacaacgaa aaccaggtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga
    16801 ttcaccgcac gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat
    16861 tcatctcccg atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tacgcccgga
    16921 gatgagcagc gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc
    16981 cgtggggatc aacaaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa
    17041 agtgatgcag gcgctggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa
    17101 tacccaggcg atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt
    17161 cctcgacgat tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct
    17221 ggcgcgtcgc aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt
    17281 gctacaacag atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt
    17341 gctgaatgaa ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc
    17401 gcgtaatccg ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta
    17461 tgctaatcgc ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta
    17521 cttccagcat cagtggcaag gctggggcaa acagcccgaa gcggcgaagt ttaacgctag
    17581 cgcaaattaa aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc
    17641 cataagcgga aagaccacag taacgcctgg taaccgacct tcacgctgcg cacattggta
    17701 atcaggcgct tatacatacc cggaagtaaa tatctcggca atcatccgtt ggcgggtaag
    17761 aatatccggc tctttgcg
//
LOCUS       DAZPFP010000068        17555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPFP010000068 DAZPFP010000000
VERSION     DAZPFP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.068"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(106..1389)
                     /gene="gltA"
                     /locus_tag="ACHUR4_002189"
     CDS             complement(106..1389)
                     /gene="gltA"
                     /locus_tag="ACHUR4_002189"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1455769.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1595..1702)
                     /locus_tag="ACHUR4_002190"
     CDS             complement(1595..1702)
                     /locus_tag="ACHUR4_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455770.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2083..2487
                     /gene="sdhC"
                     /locus_tag="ACHUR4_002191"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2083..2487
                     /gene="sdhC"
                     /locus_tag="ACHUR4_002191"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1455771.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2481..2828
                     /gene="sdhD"
                     /locus_tag="ACHUR4_002192"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2481..2828
                     /gene="sdhD"
                     /locus_tag="ACHUR4_002192"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1455772.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2828..4594
                     /gene="sdhA"
                     /locus_tag="ACHUR4_002193"
     CDS             2828..4594
                     /gene="sdhA"
                     /locus_tag="ACHUR4_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1455773.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4610..5326
                     /gene="sdhB"
                     /locus_tag="ACHUR4_002194"
     CDS             4610..5326
                     /gene="sdhB"
                     /locus_tag="ACHUR4_002194"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1455774.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5627..8428
                     /gene="sucA"
                     /locus_tag="ACHUR4_002195"
     CDS             5627..8428
                     /gene="sucA"
                     /locus_tag="ACHUR4_002195"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1455775.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8443..9660
                     /gene="odhB"
                     /locus_tag="ACHUR4_002196"
     CDS             8443..9660
                     /gene="odhB"
                     /locus_tag="ACHUR4_002196"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1455776.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9754..10920
                     /gene="sucC"
                     /locus_tag="ACHUR4_002197"
     CDS             9754..10920
                     /gene="sucC"
                     /locus_tag="ACHUR4_002197"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1455777.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10920..11789
                     /gene="sucD"
                     /locus_tag="ACHUR4_002198"
     CDS             10920..11789
                     /gene="sucD"
                     /locus_tag="ACHUR4_002198"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1455778.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11892..12614)
                     /gene="mngR"
                     /locus_tag="ACHUR4_002199"
     CDS             complement(11892..12614)
                     /gene="mngR"
                     /locus_tag="ACHUR4_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1455779.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12784..14700
                     /gene="mngA"
                     /locus_tag="ACHUR4_002200"
     CDS             12784..14700
                     /gene="mngA"
                     /locus_tag="ACHUR4_002200"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1455780.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14718..17353
                     /gene="mngB"
                     /locus_tag="ACHUR4_002201"
                     /pseudo
     CDS             14718..17353
                     /gene="mngB"
                     /locus_tag="ACHUR4_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
BASE COUNT         4256 a         4385 c         4770 g         4144 t
ORIGIN      
        1 tccgatagtg gattttattt ttacaatttt ttgtgtttag gcatataaaa attaacccgc
       61 catttgaacg gcgggttaaa atatttacaa cttagcaatc aaccattaac gcttgatatc
      121 gcttttaaag tcgcgttttt catatcctgt atacagctga cgcggacggg caatcttcat
      181 accgtaactg tgcatttcgc tccagtgggc gatccagcca acggtacgtg ccattgcgaa
      241 aatgacggtg aacatggaag acggaatacc catcgctttc aggatgatac cagagtagaa
      301 atcgacgttc gggtacagtt tcttctcgat aaagtacggg tcgttcagcg cgatgttttc
      361 cagctccata gccacttcca gcaggtcatc tttggtgccc agctctttca gaacttcatg
      421 gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa
      481 gcccatcagg cggaaagaat catttttatc tttcgcacga cgaacaaatt ccggaatgtg
      541 tttaacggag ctaatttctt ccagcatttt cagcgccgct tcgttagcac caccgtgcgc
      601 aggtccccac agtgaagcaa tacctgctgc gatacaggca aacgggttcg cccccgaaga
      661 gccagcggta cgcacggtgg aggtagaggc gttctgttca tggtcagcgt gcaggatcag
      721 aatacggtcc atagcacgtt ccagaatcgg attaacttca tacggttcgc acggcgtgga
      781 gaacatcata ttcaggaagt taccggcata ggagagatcg ttgcgcggat aaacaaatgg
      841 ctgaccaatg gaatacttgt aacacatcgc ggccatggtc ggcattttcg acagcaggcg
      901 gaacgcggca atttcacgat gacgaggatt gttaacatcc agcgagtcgt gatagaacgc
      961 cgccagcgcg ccggtaatac cacacatgac tgccattgga tgtgagtcgc gacggaaagc
     1021 gtggaacaga cgggtaatct gctcgtggat catggtatga cgggtcaccg tagttttaaa
     1081 ttcgtcatac tgttcctgag tcggtttttc accattcagc aggatgtaac aaacttctag
     1141 gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca aaataccttc
     1201 atcaccatca ataaaagtaa ttttagattc gcaggatgcg gttgaagtga agcctgggtc
     1261 aaaggtgaac acaccttttg aaccgagagt acggatatca ataacatctt gacccagcgt
     1321 gcctttcagc acatccagtt caacagctgt gtccccgttg aggttgagtt ttgcttttgt
     1381 atcagccatt taaggtctcc ttagcgcctt attgcgtaag actgccggaa cttaaatttg
     1441 ccttcgcaca tcaacctggc tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag
     1501 gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa agaatccatt gatgacgaat
     1561 aaatggcgaa tcaagtactt agcaatccga attattaaac ttgtctacca ctaataactg
     1621 tcccgaatga attggtcaat acttccacac tgttacataa gttaatctta ggtgaaatac
     1681 cgacttcata acttttacgc attatatgct tttcctggta atgtttgtaa caactttgtt
     1741 gaatgattgt caaattagat gattaaaaat taaataaatg ttgttatcgt gacctggatc
     1801 actgttcagg ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca
     1861 gcctatactg ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa
     1921 cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg cgcagttatg gaaaggacgc
     1981 tgtctgaccc gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt
     2041 taacctctgt gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg
     2101 ataagaaatg tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc
     2161 atcacggcga tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg
     2221 ggcatcctgc agtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct
     2281 tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg
     2341 gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa
     2401 acattcgaag cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca
     2461 cttctcgcag gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac
     2521 atgatttcat cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg
     2581 tcggtttttt cgccaccagt ggcgaactga catatgaagt ctggatcggt ttcttcgcct
     2641 ctgcgttcac caaagtgttc accctgctgg cgctgttttc tatcttgatt catgcctgga
     2701 tcggcatgtg gcaggtgttg accgactacg ttaaaccgct ggccttgcgc ctgatgctgc
     2761 aactggtgat tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg
     2821 gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc
     2881 aggtatgcgc gcggcgctgc aaatttccca gagcggccag acctgtgccc tgctctctaa
     2941 agtcttcccg acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg
     3001 taatacccat gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta
     3061 tatcggtgac caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga
     3121 actcgaacac atgggcctgc cgttctcgcg tctcgatgat ggtcgtatct atcaacgtcc
     3181 gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg cggcggctgc
     3241 tgaccgtacc ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac
     3301 caccattttc tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt
     3361 gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac
     3421 cgtgctggcg actggcggag cagggcgtat ttatcagtcc accactaacg cccacattaa
     3481 cactggcgac ggtgtcggca tggctatccg cgcaggcgta ccggtgcagg atatggaaat
     3541 gtggcaattc cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg
     3601 tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc
     3661 gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg
     3721 tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac tcgatcacct
     3781 gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg gagctttccc gtaccttcgc
     3841 tcacgtcgat ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg
     3901 cggtattccg accaaagtta ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt
     3961 ggttgttccg ggactgtttg ccgttggtga aatcgcttgt gtatcggtac acggcgctaa
     4021 ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt ggtcgcgcgg caggtctgca
     4081 tctgcaagag tctatcgccg agcagggcgc actgcacgat gccagcgagt ctgatgttga
     4141 agcgtctctg gatcgcctga accggtggaa caataatcgt aacggtgaag atccggtggc
     4201 gatccgtaaa gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga
     4261 tgcgatggcg aaagggcttg agcagttgaa agtgatccgc gagcgtctga aaaatgcccg
     4321 tctggatgac acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa
     4381 cctgatggaa acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg
     4441 cgcacatagc cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct
     4501 gtatctgcct gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg
     4561 cccggcattc ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga
     4621 gttttcaatt tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac
     4681 cctggaagcg gaagaaggtc gcgacatgat gctgctggat gcgcttatcc agctgaaaga
     4741 gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg
     4801 tctgaacatg aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca
     4861 gccgggcaag aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt
     4921 ggtagacatg ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa
     4981 tggacaaaat ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga
     5041 cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg
     5101 gaatcccgat aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga
     5161 tagccgtgat accgagactg acagccgcct cgacggtttg agcgatgcat tcagcgtatt
     5221 ccgctgtcac agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg
     5281 cgccatcggc catatcaagt cgatgttgtt gcaacgtaat gcgtaaactg taggcctgat
     5341 aagacgcgca agcgttgcat caggcaacca gtgccggatg cggcgtgaac gccttatccg
     5401 gcctacaagt cattacccgc aggcctgata agcgcagcgc atcaggcgta acaaagaaat
     5461 gcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga
     5521 gccactacgt agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta
     5581 gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc
     5641 tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca
     5701 gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt
     5761 ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata
     5821 tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac
     5881 caatgtgaag caggttaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca
     5941 gcatgcgaat ctagatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc
     6001 gtctttccac gatctgaccg aagcagactt ccaggaaacc ttcaacgtcg gttcatttgc
     6061 cagcggcaaa gaaaccatga aactcggcga gcttatttcc gccctcaagc aaacctactg
     6121 cggcccgatt ggtgccgagt atatgcacat caccagcacc gaagaaaaac gctggatcca
     6181 acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag
     6241 cgaactgacc gccgctgaag gccttgagcg ttacctcggc gcaaaattcc ctggcgcaaa
     6301 acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca
     6361 cgctggcaac agcggcaccc gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa
     6421 cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt tcgccggtaa
     6481 acataaagaa cacctcggca caggcgacgt gaaataccac atgggcttct cgtctgactt
     6541 ccagaccgat ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat
     6601 tgtaagcccg gtcgttatcg gctctgttcg tgcccgtctg gacagacttg atgagccgaa
     6661 cagcaacaaa gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt
     6721 ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg
     6781 tatcgttatc aacaaccagg ttggtttcac cacctctaac ccgctggatg cccgttctac
     6841 accgtactgt actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga
     6901 tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa
     6961 acgtgatgtc ttcatcgacc tggtatgcta ccgccgtcac ggccacaacg aagccgacga
     7021 gccgagcgca acccagccac tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa
     7081 aatctacgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat
     7141 ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc
     7201 gatgaacatg cactctttca cctggtcgcc gtacctcaac cacgaatggg acgaagagta
     7261 cccgaacaaa gttgagatga agcgcctgca ggaactggct aaacgtatca gtacggtacc
     7321 agaagcagtt gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc
     7381 agccggtgag aaactgttcg actggggcgg tgcggaaaac ctcgcttacg ccacgctggt
     7441 tgatgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca
     7501 ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgc tgcaacacat
     7561 ccataacggt cagggcgcgt tccgtgtctg ggactccgta ctgtcagaag aagccgtgct
     7621 ggcgtttgaa tatggttatg ccaccgcaga accacgcacc ctgaccatct gggaagcgca
     7681 gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cctctggcga
     7741 gcagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt acgaagggca
     7801 ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa
     7861 catgcaggtt tgcgtaccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc
     7921 gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc
     7981 gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga
     8041 aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta
     8101 ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat
     8161 cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca
     8221 cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag
     8281 ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgccc
     8341 ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct
     8401 ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt
     8461 ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa
     8521 cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta
     8581 ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca
     8641 acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa
     8701 accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg
     8761 gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat
     8821 ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg
     8881 gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg
     8941 gcgcaaccgg ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag
     9001 cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac
     9061 gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa
     9121 cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg
     9181 aaacgttacc cggaagtgaa cgcgtctatc gacggcgatg acgtggttta ccacaactat
     9241 ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg tgacgccggt tctgcgtgat
     9301 gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc agtcaaaggc
     9361 cgtgatggca agctgacggt tgaagatctg accggtggta acttcaccat caccaacggt
     9421 ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg
     9481 ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg
     9541 atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc
     9601 ttcctggtaa cgatcaaaga gttactggaa gagccgacgc gtctgctgct ggacgtgtag
     9661 tagtttaagc ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa
     9721 aagataacga ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa
     9781 caactgtttg cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc
     9841 gaagcagaag aagccgcttc aaaaatcggt gccggtccgt gggtagtgaa atgtcaggtt
     9901 cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc
     9961 cgtgcttttg cagaaaactg gctgggcaag cgtctggtaa cgtatcaaac agatgccaat
    10021 ggccaaccgg ttaaccagat tctggttgaa gcggcgaccg atatcgctaa agagctgtat
    10081 ctcggtgccg ttgtagaccg tagttcccgt cgcgtggtct ttatggcctc caccgaaggc
    10141 ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc tgatccataa agttgcgctt
    10201 gatccgctga ctggcccgat gccgtatcag ggacgcgagc tggcgttcaa actgggtctg
    10261 gaaggtaaac tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg
    10321 gagcgcgacc tggcgctgat cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg
    10381 atttgcctcg acggcaaact gggcgctgat ggcaacgccc tgttccgcca gcctgatctg
    10441 cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa
    10501 ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg
    10561 atgggtacga tggacatagt taaactgcac ggcggcgaac cggctaactt cctggacgtt
    10621 ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac
    10681 aaagtgaaag ccgttctggt taacatcttc ggcggtatcg ttcgttgcga cctgatcgct
    10741 gacggtatca tcggcgcggt agcggaagtg ggtgttaacg taccggtcgt ggtacgtctg
    10801 gaaggtaaca acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt
    10861 gcagcaaaag gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa
    10921 tgtccatttt aattgataaa aacaccaagg ttatctgcca gggctttacc ggtagccagg
    10981 ggactttcca ctcagaacag gccattgcat acggcactaa aatggttggt ggcgtaactc
    11041 caggtaaagg cggcactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg
    11101 ttgctgctac tggcgcttcc gcgtctgtta tttacgtacc agcaccgttc tgcaaagact
    11161 ccattctgga agctatcgat gcaggcatca aactgattat caccatcact gaaggcatcc
    11221 cgacgctgga tatgctgacc gtgaaagtga aactggacga agcaggcgtt cgtatgatcg
    11281 gcccgaactg cccaggcgtt atcaccccgg gtgaatgcaa aattggtatc cagccgggtc
    11341 acattcacaa accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag
    11401 cggttaaaca gaccacggat tacggtttcg gtcagtcgac ctgtgtcggt atcggcggtg
    11461 acccgatccc tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga
    11521 ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt
    11581 acatcaaaga gcatgttacc aagccagttg tgggttacat cgctggtgtg actgcgccga
    11641 aaggcaaacg tatgggccac gcgggtgcga tcattgccgg tgggaaaggg actgcggatg
    11701 agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg
    11761 gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc cctcgccgct
    11821 tccctctaca ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg
    11881 cctttttgtg cttatcgcga tgattttcgc tgcgctatca gggtaaattt atagtcatcg
    11941 gtattaaaag cgttgcggct atattcaaac acccgaccat caactaaata tccacgcgat
    12001 actttttcaa gaatcggctt tgtctggctg atattaagca gacggctcat ctcttcggtt
    12061 ggcatcagag gaatgatttc ctgttcgcta cgatcgataa ccatcttctt cacttcttca
    12121 ataaagtgat atttcgaatt ttccatgacc tgccaggtga gatccgggaa caacgcaagc
    12181 ggcatccagg tttcttccag cgccattggc ttttgcttgc gatagcgcac gcgcttcaca
    12241 tgccaaacac gatcctgcac ggtgatttgt agctgttgct gaagaaaatc gtcagccgga
    12301 atcacttcaa atatcagaac ttcactgtgt gtatcgacgt gacggtccga cagtttttca
    12361 tcaaaactgg ttaactgaaa aatatcgtaa ttgacccgct cttctttgac gtaagtcccg
    12421 ctgccctgaa tgctttcgag gatctgttgc tcgactaact ggcgcaaagc ctgacgcacc
    12481 gtaacccggc tgacgccaaa ctctgtttgt aatgccgatt cggtaggtaa cgcatcgcca
    12541 ggtttaagct cgccacgcgc aatttgctca cgaatgcgat cagcaatctg tcggtataag
    12601 ggtttgtgtc ccattattta gcatctcatt aatacgaatt tagccattat gcccgataaa
    12661 ctcatcctgt aaacaataca aataaaatgc aaataatttc aatcaagtga aattgattac
    12721 gcaatggtat tgttttgtcg ggcacactgg cgcgactata aaaacgatca agtgaggatc
    12781 atgatgaacc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc
    12841 agccgtgaag aggccattca cgcgttgact caacgtcttg ctgctctggg gaaaatttcc
    12901 agtactgagc aatttctgaa agaagtgtat cgccgtgaaa gccttggccc gaccgcctta
    12961 agtgaagggt tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc ggcgtttgca
    13021 gtcgccacac tcagcgagcc gcttcagtgg gaaggcgtgg atggcccgga agcggttgat
    13081 ttagtggtgt tgctggcgat tcccctcaat gaagcgggta ctacgcacat gcaactgctg
    13141 acagcgctga ccacgcgcct ggcggatgat gagattcggg cgcgtataca gtcggcgacg
    13201 acgcctgatg aattgctctc ggcgctggat gacaagggag gcacgcaacc ttctgcctct
    13261 ttttccaacg cgccaactat cgtttgcgta acggcctgcc cggcgggtat tgctcacacc
    13321 tacatggctg cggagtatct ggaaaaagcc ggacgcaaac tcggcgtgaa tgtctacgtt
    13381 gaaaagcaag gtgctaacgg tattgaaggg cgtttaacgg cggatcaact caatagtgca
    13441 actgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt taacggcatt
    13501 cctgcgcttt ctgtgcctgt tgccgagccg attcgccatg cagaagcgtt gatccaacaa
    13561 tcgcttaccc tcgagcgtgg cgatgagacg cgtaccttac agcaagatac gcaaccggtg
    13621 aaaagcgtca aaacggagct gaaacaggca ctgttgagcg ggatctcttt tgccgtaccg
    13681 ttgattgtcg cgggggggac ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg
    13741 ctacaagatc tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa actgggcggc
    13801 gggctgctcg gaattttgat ggtaccggtg ctggcagcct ataccgccta ttcgctggca
    13861 gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat gatcggctcc
    13921 gggtttctcg gcgcggtcgt tggcggattg atagccggtt acttgatacg ctggatgaaa
    13981 aatcacttgc gtcttagcag taaattcaat ggcttcctga ctttttatct ctacccggtg
    14041 ctcggtacat tgggagcggg cagtctgatg ctgtttgtgg tgggggaacc tgtcgcctgg
    14101 atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg
    14161 ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa taaagccgct
    14221 tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc
    14281 tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc accgcgcctg
    14341 tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct ggcaggtatt
    14401 accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg
    14461 ctgggctcta tggtaacggg cgctattgtc ggtgcgatga atatcggcct ttcgacaccc
    14521 ggtgctggca ttttctcgct ctttttactt catgataatg gcgcgggcgg tgttatggcg
    14581 gcaattggct ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc aattctcctg
    14641 atctggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt aatgccataa
    14701 acaaaaagga aacgacgatg aaagcggtat ctcgcgttca catcaccccg catatgcact
    14761 gggatcggga gtggtatttc accaccgaag agtcacgcat tctgctggtc aataatatgg
    14821 aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta ctcgacgggc
    14881 aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac cgcttgaaga
    14941 aactggtaga agccggcaag ttgattatcg gcccctggta tacccagacc gataccacga
    15001 ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt
    15061 ttggcgagcc gatgaaaata ggttatttac ccgattcctt tggcatgtcc gggcaactgc
    15121 cgcatatcta caatggattt ggcattacct gcaccatgtt ctggcgtgga tgttctgagc
    15181 gccacggtac ggataaaacc gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg
    15241 cgcaggtgat gccgttgggt tatgccatcg gtaagtactt acctgcggac gaaaacggat
    15301 tacgcaaacg cctcgacagt tattttgacg tgctggaaaa agcgtctgta accaaagaga
    15361 ttttgctgcc gaatgggcat gatcagatgc cattgcagca aaatatcttc gaagtgatgg
    15421 agaaactgcg cgaaatttat cctcaacgaa agtttgtgat gagccgcttt gaagaggtat
    15481 ttgagaagat cgaagcgcag cgagataatc tggcaaccct gaaaggggaa tttattgatg
    15541 gcaaatatat gcgcgtgcat cgcaccatcg gttccacgcg catggatatc aaagttgccc
    15601 acgcacgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg ctggccagga
    15661 cgttgggttt tggataccac cacggcttgc tggagaaaat gtggaaagag atcttaaaaa
    15721 atcatgccca cgacagcatc ggctgctgct gtcgtgacaa agttcatcgc gaaatcgtcg
    15781 cccgcttcga actggctgaa gacatggcgg ataatctgct gcgtttctat atgcgtaaaa
    15841 tcgccgacaa catgccgcag agcgacgccg acaaactcgt cctgtttaac ctgatgccct
    15901 ggccgcggga agaagtgatc aacaccactg tgcggctgcg cgccagccag tttaatctac
    15961 gagacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag atcgatccag
    16021 gcctaatcga tcggcaaata gttcattacg gtaattacga tccctttatg gagtttgata
    16081 tacagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc gaagcgaatc
    16141 agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gatactggaa aatgctttct
    16201 ggcaaattgc gctcaatgag gatggaacgc tacaactggt agataaagat agcggtgtgc
    16261 gctatgaccg ggtgttgcaa attgaagaaa gctctgatga tggtgatgaa tatgactatt
    16321 cacccgcaaa agaagagtgg gtaattactt cggcgaatgc gaaaccgcaa tgcgagatta
    16381 ttcatgaggc ctggcagagc agggctgtta tccgctatga aatggcggtg ccgcgtaatc
    16441 tgcaacaacg cagcgcaaga caatccactg gcagagtaga tgtcgagttg gttgtcactc
    16501 ttagtcataa caacaggcgc attgatgtgg atatcaacct tgataaccag gctgacgatc
    16561 atcgccttcg cgtcctgatc cctacatctt ttaacaccga tagtgttctg gcagataccc
    16621 agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg cagcaagaaa
    16681 gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgcagcc ttacaggaag
    16741 ggcgtaacgg catggctgtc tttagcgaag ggctacgtga atttgaagtc atcggtgaag
    16801 agaagaaaac ctttgccatt acgttgctgc gcggcgtggg cttactgggg aaagaagatc
    16861 tgcttttaag gcctaggcgg ccttcgggaa ttaaaatgcc agtcccggac tcacaactac
    16921 gtggtctgct ttcttgtcgc ctaagtttat tgagttatac cggtacgcca accgccgctg
    16981 gtgtagctca gcaggcgcga gcatggctga ctccagtaca gtgttacaac aaaatcccat
    17041 gggatgcgat gaagctcaac aaagccggat tcaacgtgcc ggaaagttat agtttgttga
    17101 aaatgccccc agtgggatgc ccctgataag cgcacttaag aaagctgaag accgacaaga
    17161 agtgatttta cggctgttta atccggctga atcagcaacc tgtgatgcga ctgttgcttt
    17221 cagtcgcgag gtgatttctt gctcagaaac gatgatggat gaacacatta ccaccgagga
    17281 aaatcaaggt tcaaatctat cggggccttt tttacccggc cagtcacgga cgttcagtta
    17341 ccggcttgcc tgaatagcaa tcaaaccgaa gccacatatg cgcggccaga ttgttgacaa
    17401 agggcgcttt gttcatgccg gatacggcat gaacgcttta ttcggtctac aaaagcaggc
    17461 aaattcaata tattgcagag atgatgtaga cactggcaag cgtagcgcat caggtaattt
    17521 tgtgtttatc ttcactctca agctacgtat atgtg
//
LOCUS       DAZPFP010000069        17397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPFP010000069 DAZPFP010000000
VERSION     DAZPFP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.069"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHUR4_002202"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHUR4_002202"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1455781.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHUR4_002203"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHUR4_002203"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1455782.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHUR4_002204"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHUR4_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1455783.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHUR4_002205"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHUR4_002205"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1455784.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="ACHUR4_002206"
     CDS             complement(5464..5661)
                     /locus_tag="ACHUR4_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1455785.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHUR4_002207"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHUR4_002207"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1455786.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHUR4_002208"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHUR4_002208"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1455787.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHUR4_002209"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHUR4_002209"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1455788.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHUR4_002210"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHUR4_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1455789.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHUR4_002211"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHUR4_002211"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1455790.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHUR4_002212"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHUR4_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1455791.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHUR4_002213"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHUR4_002213"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1455792.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHUR4_002214"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHUR4_002214"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1455793.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHUR4_002215"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHUR4_002215"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1455794.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="ACHUR4_002216"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="ACHUR4_002216"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1455795.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="ACHUR4_002217"
     CDS             complement(17102..17155)
                     /locus_tag="ACHUR4_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455796.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3944 a         4960 c         4373 g         4120 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaagaatttg gagcggg
//
LOCUS       DAZPFP010000070        17354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPFP010000070 DAZPFP010000000
VERSION     DAZPFP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.070"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUR4_002218"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUR4_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1455797.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUR4_002219"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUR4_002219"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1455798.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHUR4_002220"
     CDS             2826..3980
                     /locus_tag="ACHUR4_002220"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1455799.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUR4_002221"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUR4_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1455800.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUR4_002222"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUR4_002222"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1455801.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUR4_002223"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUR4_002223"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1455802.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUR4_002224"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUR4_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1455803.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUR4_002225"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUR4_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1455804.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHUR4_002226"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHUR4_002226"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1455805.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHUR4_002227"
     CDS             11296..11511
                     /locus_tag="ACHUR4_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1455806.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUR4_002228"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUR4_002228"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1455807.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUR4_002229"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUR4_002229"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1455808.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUR4_002230"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUR4_002230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1455809.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHUR4_002231"
     CDS             complement(16247..16786)
                     /locus_tag="ACHUR4_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1455810.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHUR4_002232"
     CDS             complement(16802..17320)
                     /locus_tag="ACHUR4_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1455811.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4018 a         4318 c         4822 g         4196 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agac
//
LOCUS       DAZPFP010000071        17144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPFP010000071 DAZPFP010000000
VERSION     DAZPFP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.071"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUR4_002233"
     CDS             <1..510
                     /locus_tag="ACHUR4_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1455812.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUR4_002234"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUR4_002234"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1455813.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUR4_002235"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUR4_002235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1455814.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHUR4_002236"
     CDS             complement(2878..3075)
                     /locus_tag="ACHUR4_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1455815.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUR4_002237"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUR4_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1455816.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUR4_002238"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUR4_002238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1455817.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUR4_002239"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUR4_002239"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1455818.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUR4_002240"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUR4_002240"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1455819.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUR4_002241"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUR4_002241"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1455820.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUR4_002242"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUR4_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUR4_002243"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUR4_002243"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1455821.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUR4_002244"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUR4_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1455822.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUR4_002245"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUR4_002245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1455823.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUR4_002246"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUR4_002246"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1455824.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUR4_002247"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUR4_002247"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1455825.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUR4_002248"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUR4_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZPFP010000072        17076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPFP010000072 DAZPFP010000000
VERSION     DAZPFP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.072"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     regulatory      33..123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            255..821
                     /gene="mntP"
                     /locus_tag="ACHUR4_002249"
     CDS             255..821
                     /gene="mntP"
                     /locus_tag="ACHUR4_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1455826.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(818..1627)
                     /gene="rlmA"
                     /locus_tag="ACHUR4_002250"
     CDS             complement(818..1627)
                     /gene="rlmA"
                     /locus_tag="ACHUR4_002250"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1455827.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1793..2002)
                     /gene="cspE"
                     /locus_tag="ACHUR4_002251"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1793..2002)
                     /gene="cspE"
                     /locus_tag="ACHUR4_002251"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1455828.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2015..2158)
                     /locus_tag="ACHUR4_002252"
     CDS             complement(2015..2158)
                     /locus_tag="ACHUR4_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1455829.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2830..3117)
                     /locus_tag="ACHUR4_002253"
     CDS             complement(2830..3117)
                     /locus_tag="ACHUR4_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1455830.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3192..3335)
                     /gene="mgrB"
                     /locus_tag="ACHUR4_002254"
     CDS             complement(3192..3335)
                     /gene="mgrB"
                     /locus_tag="ACHUR4_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1455831.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3494..3733
                     /locus_tag="ACHUR4_002255"
     CDS             3494..3733
                     /locus_tag="ACHUR4_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1455832.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3876..4667)
                     /gene="kdgR"
                     /locus_tag="ACHUR4_002256"
     CDS             complement(3876..4667)
                     /gene="kdgR"
                     /locus_tag="ACHUR4_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1455833.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4844..6217
                     /locus_tag="ACHUR4_002257"
     CDS             4844..6217
                     /locus_tag="ACHUR4_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1455834.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6263..7144)
                     /gene="htpX"
                     /locus_tag="ACHUR4_002258"
     CDS             complement(6263..7144)
                     /gene="htpX"
                     /locus_tag="ACHUR4_002258"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1455835.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7336..9384)
                     /gene="prc"
                     /locus_tag="ACHUR4_002259"
     CDS             complement(7336..9384)
                     /gene="prc"
                     /locus_tag="ACHUR4_002259"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1455836.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9404..10102)
                     /gene="proQ"
                     /locus_tag="ACHUR4_002260"
     CDS             complement(9404..10102)
                     /gene="proQ"
                     /locus_tag="ACHUR4_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1455837.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10199..10696)
                     /gene="msrC"
                     /locus_tag="ACHUR4_002261"
     CDS             complement(10199..10696)
                     /gene="msrC"
                     /locus_tag="ACHUR4_002261"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1455838.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10826..12109
                     /gene="yebS"
                     /locus_tag="ACHUR4_002262"
     CDS             10826..12109
                     /gene="yebS"
                     /locus_tag="ACHUR4_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1455839.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12078..14698
                     /gene="yebT"
                     /locus_tag="ACHUR4_002263"
                     /pseudo
     CDS             12078..14698
                     /gene="yebT"
                     /locus_tag="ACHUR4_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14778..16217
                     /gene="rsmF"
                     /locus_tag="ACHUR4_002264"
                     /pseudo
     CDS             14778..16217
                     /gene="rsmF"
                     /locus_tag="ACHUR4_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16335..16571
                     /locus_tag="ACHUR4_002265"
     CDS             16335..16571
                     /locus_tag="ACHUR4_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR1455840.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16676..16867
                     /locus_tag="ACHUR4_002266"
     CDS             16676..16867
                     /locus_tag="ACHUR4_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1455841.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(16892..>17076)
                     /locus_tag="ACHUR4_002267"
     CDS             complement(16892..>17076)
                     /locus_tag="ACHUR4_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1455842.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4120 a         4330 c         4140 g         4486 t
ORIGIN      
        1 attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt ccagattccg
       61 gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg aagactttca
      121 gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa aacccatcgc
      181 ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat ggataccccg
      241 gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt atgtcgatgg
      301 atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa ttttctgaag
      361 cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg atcggctggg
      421 gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt gcgtttgtgc
      481 tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca gatgatgaag
      541 atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg attgccacca
      601 gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac attatcgcga
      661 ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg atggttggtc
      721 gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg gtgctgatcg
      781 gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc tgccagaggt
      841 gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt tgccagactt
      901 ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct tcatcaccgc
      961 gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct tccagttgtt
     1021 ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc tccatcaaat
     1081 gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact cgtgctaatt
     1141 cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta tcggaaaacg
     1201 gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc gccgccgctt
     1261 ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac gcatcggcaa
     1321 atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg gccttttcat
     1381 caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa tgtccggcat
     1441 ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca cgagaccgtt
     1501 tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac tgatgtcgtt
     1561 ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt ggacaggaaa
     1621 acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa tcacgcggcg
     1681 ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc gcattcgacg
     1741 aggctttata gcgagattga agcgtattca cacttcagat cagtggattc gatcagatag
     1801 ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac tcaacgttct
     1861 gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt acgaacacat
     1921 ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg aaccacttaa
     1981 cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc gcccgcaggc
     2041 ataacataga taaaactgac acattactgc atgaggcacc aatataaggc tcggcagaga
     2101 agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg ccacacataa
     2161 acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta attatggcaa
     2221 gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc cacttttttt
     2281 gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt gccagttcag
     2341 gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt gttccaggcc
     2401 agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg caatgaataa
     2461 aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt accctaaaga
     2521 gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga gtgagcaaaa
     2581 aaaaatcaag agagaaacgt ttcgctaata aataatacca cgcttaatca caaatctgat
     2641 gtaaacatcc taataaatta atgaggaaaa tatctttttc tgatatcccg ttacgcaaat
     2701 aaataatatg ataattattt gaagtcagac cagagtttta atttgaagca gttgcaccat
     2761 gacagtgcgt ataatcagga tgaattgagt aacccttcaa cgacggggtt actcaagatt
     2821 gaggaaggat tatcgttcgg cgaccaggcg aataatatct tcgtcatcaa ccgatttttg
     2881 actctcaatg gtctttttat ctgctttctc tggttcgttt gcttcgcaca aacgacgtaa
     2941 cagtgcattt tgacgttttt gctgatccag caacgcctca agcagttcaa tctgttcgtt
     3001 agtccgtgaa ctggcacgat tgataaaaaa ccacaagatg agcccgataa gaagaaccac
     3061 caccgataca accaaagacg caatgttcat cacgcctgaa tttacaactt cgttcatttc
     3121 accacctctg agtagaggcg ctattctacc actgctggag aggaagaaaa tctagtgctg
     3181 aaaaaatgat atcaccacgg gataaactgg ttaatggcac aaattccgct gaaaaattgt
     3241 acatcctgat cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca agccaacacc
     3301 acgacaacca gaacgaccca tcgaaacttt ttcactccaa tctccgcttc acctacctta
     3361 tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac tatgtcgata ttcgtgcttt
     3421 ttcggaatga gtagcttgct tcagcgcata ttgccgctac gattaagcga acataaaaag
     3481 agaagagatt gtaatgcgat tcatcattcg cacagttatg ctgatagcac tggtatggat
     3541 aggattatta ctcagcggat atggcgtttt gattggaagc aaagagaatg ccgcaggatt
     3601 aggtttgcaa tgtacctatc tgactgccag aggcaccagc acggtacaat acttgcacac
     3661 taaaagtggt ttcttggaga taacagattg cccactgtta cgtaaaagca atatcgtggt
     3721 cgataatggt taagggtaat aaataaccag attctcactc atgagtaaaa tattcttata
     3781 gcaagcgcgt ttaatataac gcaccttcaa ctttattttt ctcatgctaa ataaagaacc
     3841 tgtctgtttg atattccgac aggttcttac tctcatcaga acggatagtc gtgataaccc
     3901 atttgggcag aaattttgcg cgctgcggta tgcaacattg cgacatattc ctgtaaacgc
     3961 tcttcagaga aacgcaacgt cgggaaggag atgctcaaac cggcaatgac cacgccaaag
     4021 cgatcaaata ccggtaccgc aatgcattgc agcccttctt cctgctcttc gttatcttct
     4081 ccatacccct gctcgcgcac ctggtccaga acgggtaata acgcttctgt actggtgatg
     4141 gtccgctcgg tactgcgttt atactccacg ccctcaagaa tttgcttcac ttcatcgcga
     4201 tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt acagcggatt acgacgccca
     4261 atccgtgaat acatgcgcaa attgtacata gagtcaattt tgtgaatgta aacaatactg
     4321 tcttcgtcca gtgcgccgag gtggatagtt tctttggtca ggcgggagat ctcacgcatc
     4381 tggatatctg cgctacgaat taaatcgacg ttttgtaacg cgcgagcgcc cagttcaaac
     4441 aatttcaggg tcagggaata tttctccgac tccccttcct gcgccacata acctaaggtt
     4501 ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg ctgcgacagc
     4561 tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa aacttttagc
     4621 acggaagata cagaatcagg ctgtttatcc agatctgcgt tagccattta tcacctcatt
     4681 gcgagtgttt tataaaaatc agaactgttt tttattataa tttcgcacca gggtggtcgc
     4741 aatccatctt ttgccggtta gttacaattc tgcgacatcc accgtgaata tcagtgctag
     4801 aatcataccc ctgttgatta ttcaccaaag atataaaatt cctatgccaa aagttcaggc
     4861 cgacggcctg ccattgcccc agcgatacgg tgcgatatta accattgtga ttggtatttc
     4921 gatggctgtc cttgacggcg caatcgccaa cgtcgccctg ccaacaattg ccacggacct
     4981 tcatgccaca ccagccagtt ccatctgggt agtgaacgcc tatcaaatcg ccattgtcat
     5041 ctccctgctc tcgttttcgt ttctgggcga tatgtttggc tatcgacgta tttataaatg
     5101 cggtctggtc gtttttctgt tgtcttcact gttctgcgcc ctttctgatt cgctgcaaat
     5161 gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga gcgttaatac
     5221 cgcacttatc cgcctgatct atccacaacg ttttctgggg agagggatgg gcataaactc
     5281 gtttattgtt gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa tcctctccat
     5341 cgcatcctgg aaatggttat ttttaatcaa cgtaccgtta ggtattatcg ccctgcttct
     5401 ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt aaaccccgtt tcgacctgcc
     5461 cagcgccgtg atgaacgcgt taaccttcgg cctgcttatc actgcgttga gtggtttcgc
     5521 tcaggggcaa tcgctgacgt taattgctgc ggaactggtg gtaatggttg ttgttggtat
     5581 tttctttatt cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg atttactgcg
     5641 tatcccgctg ttttcacttt ctatttgcac atctgtttgc tctttctgcg cacaaatgct
     5701 ggcaatggtt tccctgccct tttacctgca aaccgtgctc gggcgtagtg aagtcgaaac
     5761 aggtttactt ctgacaccgt ggccgttagc aacgatggtg atggctcctc tggcaggcta
     5821 tttgattgaa cgcgtacatg caggattgct gggtgcttta gggttgttca tcatggctac
     5881 ggggcttttt tccctggttc tgctgcccgc gtcacctgcg gatatcaata ttatctggcc
     5941 gatgatctta tgtggcgctg gatttggctt attccagtca cccaataacc acaccattat
     6001 tacctccgct ccgcgcgaac gtagcggtgg ggccagtggc atgttaggaa cggctcgtct
     6061 actgggtcag agtagtggcg cggcgctggt ggcgctgatg ctaaatcagt ttggtgataa
     6121 tggtacgcac gtctcgctga tggctgcggc tattctggca gtgattgctg cctgtgtcag
     6181 tggtttacgt atcactcagc cacgagccag ggcataaaaa aagcgcgtcg atcaggacgc
     6241 gctttttagt atttacttca tattacttca ggtattcacc cgtacgcaga gcttcaatac
     6301 gtttatccag cggcgggtga gtcatgaaca actcactgag cgatttcgac ttaccgttaa
     6361 tgcagaaagc catcatgctg gttgcttctt gcggttcata gctggttttc aggcgctgca
     6421 gcgcggcaat cattttctcg cgaccaacca gttttgccga accggcatct gcatggaatt
     6481 cacgatgacg cgagaaccac atggtgataa tgctcgccag aataccaaac accagttcca
     6541 gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt gctctcttca ccttcatcac
     6601 gatttccgcc cataaaaccc gcggcaagct gcgccagaat acgggaaata aagataacga
     6661 aggtgttcac cacgccctga atcagcgtca tggtgaccat atcaccattg gcgatgtggc
     6721 tgatttcgtg agcaattacc gcctcggctt catccgggct catgttctgc agcaaaccgg
     6781 tgctgacagc aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg ttgatgtccg
     6841 gcgcatggta gatagccact tgcggcatag cgatccccgc ctgacgagcc tgggttgcta
     6901 cagtattgac cagccaacgt tccctttcgt tacgcggttg ctcgatcact tccccgccaa
     6961 cagatcgtaa tgccatccat ttggacatca gaagcgaaac gaaggaacca ccaaaaccga
     7021 acagcaaggc catcatcatc agcccctgaa cgctgctcga ctgtatccct gtcaggctca
     7081 gtaccagccc gaaaacgacc attacggcca ggttcgttag caggaagagc gcgattcgca
     7141 tcataatttt ctttttacct cagtttaaca aaacgcaata tgcgataccc acatcgtatg
     7201 ggttacgcga ctattttcaa gtctggatag tgcgtaagtc accagaaaga cacaacttta
     7261 cattttgtag catctgattt acggcatctt gccgctgtta aaaaatcagg cacaatttct
     7321 tgtgcctgat tgatattact tgacgggagc gggttgttcc gcgggtctgg ctttttcaag
     7381 cttcgccaga tcgagtgcga tattcaccgt ctcatccaga taaggatccg gctcctggta
     7441 atctttcgat agatcatcca gcttcttcaa ctccggttta ccttctcgtt taaagcgttc
     7501 gttcaaacgc gccagacgcg tcgcatcatc ttcattattc tctttctcac gcacagcgta
     7561 gttcagagaa acgatattgc gcttgtcctt catagcgttg aagcgcgcga tatccttcat
     7621 gatgttctgg aactcaggat ctttcgcgat acgcgcatta tgttccttca gcagctccgg
     7681 ttcaaaggca gttaaatctc ctgatttcac ataagtcgcg gcatcgatgc tatcccacgg
     7741 cagcgcgtta tcttcgaatt tctcacccgt ttccgtttct tcattacccg tcggcatgat
     7801 gatgtctggt gttacgcctt tacgttgcgt actgccgccg ttaacgcgat agaatttctg
     7861 gatcgtgtac tgcacagaac ccagcgctgg ccattcagga cgtaacatct gatcgtaaat
     7921 acggttcaat gaacggtatt gctgaacggt gcctttacca aacgtcggtt cacccacaac
     7981 cagcgcacga ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg aagcactgaa
     8041 acggtcaacc agtaccacca gcgggccttt atagaacacc tgcccgtcgg tatcgctgtc
     8101 ttcacgaacc ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag gaataaacag
     8161 acctgagagc gataccgctt cggtcaacgc cccaccgcca ttgctacgca ggtcgatgat
     8221 cacactgctg acattctgtt tttccagttt ctgcagttga actttgacat cgtctgtcaa
     8281 acccacatag aagcccggaa tatccagcac gccgactttc tctttaccga cggtcttcac
     8341 cgacatttta accgcgcggt cttcgagacg aatacgttca cgggtcaacg ttacagtacg
     8401 ggtcttggtc cctttaccag caggtaaaat ttccagacga actttactgc ccttcggccc
     8461 tttaattaag gcaaccacat catcaagacg ccagccaatc acgtcaacca tcggcttgcc
     8521 tgtttgacca acaccgacaa ttttgtcacc aacgctgata gctttactct tcgctgccgg
     8581 accacctgcc accatcgaat tgataacggt gtagtcatca tccatttgca gcactgcgcc
     8641 aataccttcc agtgacaaac tcatttcagt gttgaactgt tcggtattac gcggggaaag
     8701 atagttggta tgcggatcga tttcacgcgc aaacgccgtc attgccagcg agaaaacatc
     8761 ttcgctgttg gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg tcagggtttc
     8821 acgaatttct ttatccgttt ttcctgtcag cttcaggctt aactcgtcga atttgacttt
     8881 actgtcccac tgcgcgttca actcagcctc gtttttcggc cagggcgctt tgctgcggtc
     8941 aaggttataa gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg acaaagcgta
     9001 ctggtaacgt tcaaaacggc gcttttgcgc cagattgtag agatcgtaga aaacgtcgag
     9061 tttgcctgaa cgcagttcat cgcctaactc ggttttcttt ttcgcgaact gttcaacatc
     9121 gcttgccagc agcacattgt ggctgtaatc gagcagattc aggtagcggt caaagatttt
     9181 ggccgaaaat gcctgatcga ggtcgaactg gcgataatga gaacgggtga agcgcgacgt
     9241 tacgcgctca cttaccgtcg catgctgcgt ctcttccttt aataccggaa tttgatcagc
     9301 acgcgtgata tcttctacag cgaaggtctg gcctgctatt gcaagcaggc cagctaacgc
     9361 ggtaagccta aaaaacatgt tcatgcctgg cccggcctcc gtttcagaac accaggtgtt
     9421 ctgcgcgcac aatcaaagac atacccgaat tcagctggac gcggacgccg tctttggtga
     9481 tttctaatac ggtggcatcc atcgcgtttt gacccgcttt caccttcagg gcttgtccga
     9541 cagtcagagc tgaaatgtca gaaaccgggg tgtgctgttc ttcgcgaggt gcttttactg
     9601 tttttggcgc tttctctacc ggcttttgcg cacgaggttt acgttcagcg ccttctttgc
     9661 ggcgtggcgt ggtcggacgt ggcttgcgtt cgcggcgcgg tgcgtcttct ttctcaccag
     9721 cagctgcggc agcttcgcgt tttttcgctt gctgttcagc acgctgtgcc tgaacacgcg
     9781 ctttcgcttc ttcaagctgc ttgcgagcat gctctacatg ttgctcgtcc agctcaccgc
     9841 atgggttgcc gtcaagatcg acacgcgttg cgccgggttt aacaccgtaa agataacgcc
     9901 agctcgaagt gtagagacgt aaagcggatc gtaattgcgt tttgctcagg ttcatttccc
     9961 cggcaacacg atcgaccaaa tcctgaaaaa taccaatttt cagcggacgc gcttcacctt
    10021 ccgcactgaa acagtgggga aaacgttcgg ccagaaacgc gattacttct ttactgctat
    10081 tcaacttagg ttgattttcc atgaaatttc ctgattacaa cggacgtagc caacaagcgc
    10141 aggcatgaac aggcgtcatt ataatgacgc taccagtaaa tgctacgtta tccgttgatt
    10201 atcctgcgac gctcgcaaag aattttttgt aatccgtcgt tgcaagcact ttttcaagct
    10261 gtgccacaag ctgacgtaag ccttgctcgt cctcgtctgt aaagcgagcg aagacagtac
    10321 tatcgatgtc gagaacacca ataatctgat ttttcaccac cagcggcaga acaatttcag
    10381 aattactcgc cgcatcacag gcaatatgcc cgtcaaacgc gtgaacatcc tcgatacgct
    10441 gcacttgatt gcgggcaacc gctgtgccgc acaccccgcg cccgacgggt atccggacac
    10501 aggcaatttt gccctgaaat ggtccgagta ccagtgtatc gtcctcaagc aaataaaaac
    10561 ctgcccagtt aacgtcagtg agacgctcgt ataacaacgc actggtgttc gcaagcgttg
    10621 ccagaaaact ggtttctccc gccatcagcg cgttaaagtc gcgatttaaa tccgcgtaaa
    10681 attctgtttt gttcattact taatcacttg gttgtcttac ctggaactgc cagcctatta
    10741 aaataagcat taaatgcgtt aatgctcaag atcattccca tcatgggtta agattaatgt
    10801 taattcttat tacatttggc acgtcatggc tcttaacaca ccacaaatta cgccgacaaa
    10861 aaagataaca gtgagggcaa tcggcgagga actgccgcgt ggtgattacc aacgttgccc
    10921 gcaatgtgac atgctgttta gcctgcccga gataaattct catcaaagtg cctattgtcc
    10981 gcgctgtcag gcaaaaattc gcgacgggcg cgactggtcg ctaacgcgcc tggcggcaat
    11041 ggccttcacc atgctgttgc ttatgccgtt tgcctggggc gaaccgctgt tgcatatctg
    11101 gctgttaggc attcgtattg acgccaacgt catgcaaggc atctggcaaa tgaccaaaca
    11161 gggcgatgcg ataacgggat caatggtctt tttctgcgtt attggtgccc ccctcattct
    11221 ggtgtcctcc atagcatatt tatggtttgg taaccgactg ggaatgaatt tacgtccggt
    11281 actgctgatg cttgagcgac ttaaagagtg ggtaatgctg gatatctacc tggtcggcat
    11341 tggcgttgct tctataaagg tacaggatta tgcccatatc caggcgggtg tcggcttgtt
    11401 ctcttttgtg gcgttggtga ttttaacgac ggtgacgttg tcacatctta atgttgagga
    11461 gctgtgggag cgatattatc cgcagcgccc cgctacgcgt agggacgaga aacttcgtgt
    11521 ctgtcttggg tgccatttta ccggctatcc tgatcagcgt ggtctctgcc cgcgttgcca
    11581 tatcccgcta cgcctgcgtc gtcgtcatag tttgcaaaaa tgctgggcgg cgctgttagc
    11641 gtcaatcgtt ttgttgttac cagccaacct attgcctatt tctatcattt atctgaatgg
    11701 tggccggcag gaagatacga ttctttccgg aattatgtcg ctggcaagta gcaacattgc
    11761 ggttgcggga atcgtgttta tcgccagtat tctggtaccg tttactaaag tgatcgtcat
    11821 gttcacttta ctgttgagca ttcattttaa atgccagcaa ggtttacgca cacgcattct
    11881 gttactgcgg atggtgacct ggattggacg ctggtcgata ctcgacctgt ttgtcatatc
    11941 tttaaccatg tcgctgatta atcgcgatca gatcctcgct tttactatgg gaccggctgc
    12001 gttttatttt ggcgctgccg taattttgac tattcttgct gtggaatggc tggacagccg
    12061 cttactttgg gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa gcgcagatta
    12121 aaaataaacg ccgtatctca cctttctggc tgctgccttt catcgcgcta atgattgccg
    12181 gttggctgat ttgggacagt tatcaggacc ggggtaatac cgtcaccatc gactttatgt
    12241 cggcggatgg tattgttccg ggccgtacgc ctgttcgtta tcagggcgtt gaagtcggaa
    12301 cagtgcagga tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag gtcagcatca
    12361 agtccgatat gaaagatgcg ctgcgcgaag agactcagtt ctggctgatg acgccaaaag
    12421 catcgttggc aggtgtctcc gggctggacg ccctcgtcgg tgggaactat atcggcatga
    12481 tgccgggtaa aggtaaagag caggatcact ttgtcgcgct cgatacccaa ccgaaatatc
    12541 ggctggacaa tggcgatctg atgatccacc tgcaagcccc cgctctcggt tcgctgaaca
    12601 gcggttcatt ggtctatttc cgcaagatcc cggtgggaaa agtctacgac tatgccatca
    12661 atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt accgatctgg
    12721 tgaaaaaagg tagccgtttc tgaaacgttt ccggcgttga tgccaacgtc agtatcagtg
    12781 gcgcgaaggt gaaactggaa agtctggcgg cactggttaa cggtgcgatt gccttcgatt
    12841 cacctgaaga gtcgaaacct gccgaggcgg aagatacctt tggtctgtat gaagatctgg
    12901 cccacagcca gcgtggagta ataataaaac tggaactgcc gagtggagca ggattaaccg
    12961 ccgactcgac gccgttaatg tatcaggggc tggaagtcgg acagctgact aaactggatt
    13021 taaatcctgg tggtaaagtc actggggaaa tgaccgttga tccctgcttc gtgaaaatac
    13081 ccgcatcgaa ttacgcaacc cgaaattatc ccttagcgat gctaatctca gcgccctgct
    13141 gaccggaaaa acctttgagt tggtgcccgg cgatggcgag ccacgcaaag agttcgttgt
    13201 tgtgccgggc gaaaaagccc tgctgcaaga gccggacgtt ctgacactga ccctgacagc
    13261 accggaaagt tacggtattg atgcgggtca gccgctcatt cttcacggcg tgcaggtagg
    13321 ccaggttatc gatcgtaaac tcaccagcaa aggcgtcacc tttaccgtcg ccatcgagcc
    13381 tcagcatcga gaactggtaa aaggcgatag caaatttgtc gtcaacagcc gtgtcgacgt
    13441 gaaggtgggg ctggatggcg ttgagtttct cggtgccagc gcctcagaat ggattaacgg
    13501 cgggatacgt attctgccgg gcgataaagg cgagatgaaa gccagctatc cactgtatgc
    13561 caatctggaa aaagcgctgg agaacagcct tagcgattta cccaccacaa ccatgagttt
    13621 gagtgcagag acgctgccgg atgtgcaggc aggatcggta gtgctgtacc gtaaatttga
    13681 agttggtgaa gttattaccg tgcgtccgcg agctaacgcg ttcgatatcg atctgcacat
    13741 taaaccggaa tatcgcaacc ttttgaccag caacagcgtg ttctgggcag aaggcggggc
    13801 gaaagttcag ctgaatggta gtggcctgac cgtacaggca tccccgctct ccagagcatt
    13861 aaagggggcc attagcttcg ataacctcag tggtgccagc gccagtcagc gtaaaggcga
    13921 caagcgaatt ctgtatgctt ccgaaacagc ggcccgtgcg gttggcaggc agattacgct
    13981 tcacgctttc gatgccggaa aactggcggt cgggatgcca attcgctatc tcggtattga
    14041 tatcgggcaa atccagacgc tggatctgat taccgcacgc aatgaagtac aggcaaaggc
    14101 ggtgctctat ccggaatatg tccagacctt tgctcgcggt ggtacgcgct tctcagtggt
    14161 cacaccgcaa atctcggcag ctggcgttga gcatctagat actatcctcc agccgtatat
    14221 caacgtcgaa ccaggccgtg gcaatcctcg tcgcgacttt gaattagaag aggccaccat
    14281 tactgattcg cgttacctag atggtttaag cattattgtt gaagcgccgg aagccggttc
    14341 gttaggcatc ggtacgcctg tgctgttccg tggtctggaa gtaggtacgg ttaccggaat
    14401 gacgctgggg acattgtcag atcgcgtgat gattgcgatg cgcatcagta aacgctatca
    14461 acacctggtg cgtaacaatt ccgtcttctg gttggcatcg ggttacagtc tggactttgg
    14521 tctgacgggc ggcgtagtga aaaccggcac ctttaaccaa tttatccgtg gcggcatcgc
    14581 cttcgccacg cctccgggta cgccacttgc accgaaagcc caagaaggca aacacttcct
    14641 gttgcaggaa agtgaaccga aagagtggcg tgaatgggga actgcgcttc ccaaataatg
    14701 cccactgctc cggcgtgcct gcgccggagc gcttatgcta aactgcgcgc ctgttttttt
    14761 gccagtggta catgctcgtg gcccaacaca ccgtttattt cccggacgcg tttctgacac
    14821 aaatgcgcga agctatgcct tcgacgctct catttgatga ttttcttgcc gcctgtcagc
    14881 gcccgttgcg ccgcagcatt cgcgttaata cgctgaaaat ctccgttgct gatttcctgc
    14941 aattaaccgc tccttatggc tggacgctta cgccaattcc gtggtgtgaa gaaggtttct
    15001 ggattgaacg cgacaatgaa gatgcattgc cattgggtag taccaccgag catttaagtg
    15061 gcctgtttta tattcaggaa gccagttcaa tgttgcccgt cgccgccttg tttgctgacg
    15121 gtaatgcacc acagcgggtg atggatgtcg ctgctgcgcc aggctcaaaa acgacgcaaa
    15181 ttgccgcgcg gatgaataac gaaggggcaa tccttgccaa tgagttttcc gccagttggg
    15241 taaaagtgtt acatgccaat atcagccgct gtggcatcag taatgttgcg ctcacacatt
    15301 ttggtggccg cgtgtttggt gcggcagtgc cagaaatgtt tgatgccatt ttgctggacg
    15361 ctccctgctc cggcgaaggc gtggtgcgta aagatcccga tgcgctaaaa aactggtcac
    15421 ccgaaagcaa tcaggaaatc gcagctaccc aacgggaact gattgacagc gcctttcatg
    15481 cattacgtcc tggcggtacg ctggtttact cgacctatac cttaaaccgg gaagaaaacg
    15541 aagccgtttg ccggtggctg aaagagactt accccgacga agtagagttt ttaccacttg
    15601 gcgatctctt ccctggtgca aacaaggcgc tgaccgaaga aggttttttg catgttttcc
    15661 cacaaattta cgactgcgaa ggcttcttcg ttgctcgtct gcgtaaaact caggcgatcc
    15721 ccgccttacc cgccccaaaa tacaaagtgg gcaatttccc gttcagcccg gtgaaagatc
    15781 gcgaagccgg acaaattcgt caggcggctg caagtgttgg cttaaactgg gatggaaacc
    15841 tgcgactctg gcaacgcgac aaagaactgt ggttgttccc ggtaggcatt gaagccctga
    15901 tcggtaaagt ccgattttct cgcttaggga ttaaacttgc cgaaacgcat aacaaaggtt
    15961 atcgctggca gcatgaagcg gttattgccc atgcctcccc cgacaatgta aacgcttttg
    16021 aactgacacc gcaggaagcg taggagtggt atcgcgggcg cgatgtttac ccgcaagccg
    16081 cgccagtggc ggatgacgtg ttggttactt tccagcatca gccgattggt ttagccaaac
    16141 ggattggttc gcgactgaaa aacagctacc cgcgtgaact ggtgcgggac gggaaacttt
    16201 ttaccagtaa cgcatgacag cgcacaaaaa aagcgcactt tttgactggc acattcgccg
    16261 gcctcaacta ggctgaaaaa ttgtgcgatc ggactggtcg tactacaatc agcagctaaa
    16321 tggagagcac gaagatgaaa accagtgtgc gcataggcgc ttttgaaatc gacgacggcg
    16381 aattacacgg tgaatcgccg ggtgatcgaa cgttaaccat tccttgtaaa tctgaccccg
    16441 atttatgtat gcaactggat gcctgggatg ctgaaaccag tatccctgcc ctgctaaatg
    16501 gcgaacactc tgtcctttac cgtacccgtt acgatcaaca atctgatgcc tggattatgc
    16561 gtcttgcctg atccaaaaag aacccgtcgg catggcgggt tatttgtcct ggttattccc
    16621 ccgttgtaaa atctctccta aacttaacgg tacggcacca cacttcgggg atgaaatgtt
    16681 cgcgctggta ctttttgttt gctacctgga tggcggttgt gaagatattg ttgtggatgt
    16741 ctacaacacg gaacagcagt gtctttattc tatgagcgat caacggatcc gccatggcag
    16801 ttgttttccg attgaggatt ttatagatgg tttctggcaa ccagcacagg agtatggtga
    16861 tttttaatta ttgcagttgc acaagagtca gttagccccc aaagacagca ccggtatcaa
    16921 tataatgcag gttgccaata tccacgcgat gtcgcaacgg tgtatgacca aaccagaaat
    16981 gatcagcacc tgtaattccc tgcccttttg ggcgttcacc taatcgcgag cggctccaca
    17041 agacctgatg caaatcaacg tccttttgcc attcat
//
LOCUS       DAZPFP010000073        16358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPFP010000073 DAZPFP010000000
VERSION     DAZPFP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.073"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            73..712
                     /locus_tag="ACHUR4_002268"
                     /pseudo
     CDS             73..712
                     /locus_tag="ACHUR4_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            815..1138
                     /locus_tag="ACHUR4_002269"
     CDS             815..1138
                     /locus_tag="ACHUR4_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR1455843.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1205..2662)
                     /gene="nuoN"
                     /locus_tag="ACHUR4_002270"
     CDS             complement(1205..2662)
                     /gene="nuoN"
                     /locus_tag="ACHUR4_002270"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1455844.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2669..4198)
                     /gene="nuoM"
                     /locus_tag="ACHUR4_002271"
     CDS             complement(2669..4198)
                     /gene="nuoM"
                     /locus_tag="ACHUR4_002271"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1455845.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4362..6203)
                     /gene="nuoL"
                     /locus_tag="ACHUR4_002272"
     CDS             complement(4362..6203)
                     /gene="nuoL"
                     /locus_tag="ACHUR4_002272"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1455846.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6200..6502)
                     /gene="nuoK"
                     /locus_tag="ACHUR4_002273"
     CDS             complement(6200..6502)
                     /gene="nuoK"
                     /locus_tag="ACHUR4_002273"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1455847.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6499..7053)
                     /gene="nuoJ"
                     /locus_tag="ACHUR4_002274"
     CDS             complement(6499..7053)
                     /gene="nuoJ"
                     /locus_tag="ACHUR4_002274"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1455848.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7065..7607)
                     /gene="nuoI"
                     /locus_tag="ACHUR4_002275"
     CDS             complement(7065..7607)
                     /gene="nuoI"
                     /locus_tag="ACHUR4_002275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1455849.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7622..8599)
                     /gene="nuoH"
                     /locus_tag="ACHUR4_002276"
     CDS             complement(7622..8599)
                     /gene="nuoH"
                     /locus_tag="ACHUR4_002276"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1455850.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8596..11322)
                     /gene="nuoG"
                     /locus_tag="ACHUR4_002277"
     CDS             complement(8596..11322)
                     /gene="nuoG"
                     /locus_tag="ACHUR4_002277"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1455851.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11375..12712)
                     /gene="nuoF"
                     /locus_tag="ACHUR4_002278"
     CDS             complement(11375..12712)
                     /gene="nuoF"
                     /locus_tag="ACHUR4_002278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1455852.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12709..13209)
                     /gene="nuoE"
                     /locus_tag="ACHUR4_002279"
     CDS             complement(12709..13209)
                     /gene="nuoE"
                     /locus_tag="ACHUR4_002279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1455853.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13212..15014)
                     /gene="nuoC"
                     /locus_tag="ACHUR4_002280"
     CDS             complement(13212..15014)
                     /gene="nuoC"
                     /locus_tag="ACHUR4_002280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1455854.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15108..15770)
                     /gene="nuoB"
                     /locus_tag="ACHUR4_002281"
     CDS             complement(15108..15770)
                     /gene="nuoB"
                     /locus_tag="ACHUR4_002281"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1455855.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15786..16229)
                     /gene="nuoA"
                     /locus_tag="ACHUR4_002282"
     CDS             complement(15786..16229)
                     /gene="nuoA"
                     /locus_tag="ACHUR4_002282"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1455856.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4053 a         4622 c         4277 g         3406 t
ORIGIN      
        1 gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac gccttttatt
       61 gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt cactccctct
      121 gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca cgtaagtcct
      181 gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg taatgccggc
      241 aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt cagtagcggt
      301 gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc ctggaagcca
      361 gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact ttattgctgt
      421 ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat tattgtcatc
      481 ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta ttgatttttc
      541 tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg acaccgcttt
      601 ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga attatcagaa
      661 aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat aataaaaatt
      721 tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt ctgttttaag
      781 atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct attttgcaga
      841 aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa
      901 tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa gtgagctgcg
      961 taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc
     1021 agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa agctgattag
     1081 cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg
     1141 gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactaa
     1201 cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt ggccatacac
     1261 ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc
     1321 agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg ctcaccgcca
     1381 cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc
     1441 acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag cccagcgtca
     1501 tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg atcggacgat
     1561 gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg
     1621 acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa
     1681 cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc accagcagat
     1741 agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc tggctcaacg
     1801 ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact acgcgaatcg
     1861 cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga
     1921 ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg
     1981 gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca atcatcatgc
     2041 cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt ttacccagcg
     2101 cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac agcaggaaag
     2161 aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc
     2221 ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca
     2281 gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca attaacacca
     2341 acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac
     2401 aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc
     2461 gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc gaaaccagcg
     2521 ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa
     2581 tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca
     2641 ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa
     2701 accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc tgcggataga
     2761 agcccagcag taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca
     2821 tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgca
     2881 acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca
     2941 ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc
     3001 caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc catttcatct
     3061 tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac
     3121 cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg attaccgcgc
     3181 cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc atgtgggaaa
     3241 ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc cacgcaccgt
     3301 agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc gacgcgttcg
     3361 ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc aagatccccg
     3421 cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc cagccatgca
     3481 gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc aacagatatt
     3541 ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg
     3601 tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc agaccactcg
     3661 cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg tcagaggctt
     3721 tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga
     3781 agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca
     3841 ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat agtaccgcca
     3901 gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca atggcgagat
     3961 gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa
     4021 ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca
     4081 gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg
     4141 tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt agtaacatgg
     4201 cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat tctcacgaca
     4261 gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc ttatccgacc
     4321 tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag taccatcaac
     4381 agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg cagataaccg
     4441 ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg gatgttcatc
     4501 attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg cttgacgaac
     4561 actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact cagcaggcgg
     4621 cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca cagccaggct
     4681 gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt caacatgctg
     4741 ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac aatcagtgcg
     4801 ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt tacccctttc
     4861 acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat acggaaggtg
     4921 tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat atgaccattc
     4981 gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac cagcggcagt
     5041 gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat agatttacgc
     5101 agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac ggaaccggat
     5161 gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc
     5221 catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt agagtaagcg
     5281 agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc
     5341 gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag gccgtgggta
     5401 cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag cgcggagaca
     5461 ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg cgcagattta
     5521 ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt gccgtcagca
     5581 aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag ttcgttgtaa
     5641 agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac gaacgctttc
     5701 attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag ataggagcac
     5761 aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag aaccagaact
     5821 accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg
     5881 cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac caccgagagc
     5941 atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac
     6001 atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa atcaacgccg
     6061 ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat cgccgaaacg
     6121 ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa tggcaaaata
     6181 atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg ttcaggttct
     6241 ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct gccgccgcga
     6301 ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag cttccggcga
     6361 ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc ataaacagca
     6421 gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc gcgaggatca
     6481 gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt
     6541 acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag
     6601 acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc cgaacagcgt
     6661 aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt taacaccgag
     6721 gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc caatccacac
     6781 ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt tgagcatcat
     6841 caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt ccagcgcacc
     6901 ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg aaataatcag
     6961 gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg caagtatggc
     7021 tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg taacaggctc
     7081 ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc gtcgattgcc
     7141 atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga gatcagcaga
     7201 tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa atccggggtt
     7261 aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat gcagcgtgag
     7321 aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc tttttgcagc
     7381 gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca acgctcttcg
     7441 ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag atagaccggc
     7501 tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat catccagata
     7561 ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt cttattgccc
     7621 cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc agcgtcagcg
     7681 gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc ggtaacgacg
     7741 cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac cagatgaatg
     7801 gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat gcagagatgg
     7861 tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg gaatattcaa
     7921 tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg tgacggtgac
     7981 acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg atgacgttcc
     8041 acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca
     8101 cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca gaagcacgca
     8161 tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa
     8221 ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc
     8281 aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag gcaatcatcg
     8341 gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct ttaaagaaca
     8401 ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg ttaggtccat
     8461 aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat gccccacagg
     8521 tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc tcaatcagtt
     8581 ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg cgccagccag
     8641 caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg tcagtccttc
     8701 ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga cgcgtgtacc
     8761 tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg gctgcggcat
     8821 acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca ggtggtagta
     8881 cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta
     8941 atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa agcgcagttt
     9001 gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc ccggcgccca
     9061 ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca tcgagaaggt
     9121 gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat tggcgcgcat
     9181 ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac gaatacggaa
     9241 tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg ccacaacagc
     9301 gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg tgctgtgcag
     9361 cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat aagccggatc
     9421 gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac cgtcgctttc
     9481 agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg tgcgttgatg
     9541 atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg tagcagaagc
     9601 gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg tttccagttc
     9661 ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc tgttgacgga
     9721 acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt
     9781 agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa tcaacggttt
     9841 cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct caggttcgat
     9901 accgtcaacc gctggtgcgg agttatccag cgcatgggca atggcaaaac ctaaacgcgc
     9961 ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac gagtgtcatc
    10021 aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga ttgccgcaat
    10081 ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt tcaccgcctg
    10141 acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac
    10201 cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt cacgcagcac
    10261 tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt agaagttttc
    10321 ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat
    10381 accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg cctgttcggc
    10441 gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac gatccttcag
    10501 gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc
    10561 gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc tgatgttaca
    10621 gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt tacggttgta
    10681 acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga ccaggttacc
    10741 ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc
    10801 gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca cacagcggta
    10861 gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct gattacggtg
    10921 ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg
    10981 aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt tggtcattaa
    11041 ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt
    11101 gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt cttccgcgtt
    11161 ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg ccggatgcca
    11221 gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt tgtccgctcc
    11281 gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc ttccagtttt
    11341 ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc agcaggttcg
    11401 gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc gcctcaaatt
    11461 cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt gcgtgggcac
    11521 agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg atatcgcccg
    11581 gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc agaccgtcgc
    11641 ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg ttacgcacca
    11701 gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc agacggctgc
    11761 ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg gtcaggaagt
    11821 cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc ataccaccgg
    11881 cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac agtcccggat
    11941 ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt ttcgagatgt
    12001 tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg gtttcgacgt
    12061 tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg ggtttcgagc
    12121 gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc ccgcagatgt
    12181 agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc ataatgtttt
    12241 tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt actgccgctt
    12301 cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca aacgcggaga
    12361 tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg tctttatagg
    12421 tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg gattcgtctt
    12481 tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc cctttcagac
    12541 cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc gccttacgcg
    12601 ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc
    12661 gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc atttataccg
    12721 ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct catcgatcat
    12781 catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg taaagcggcc
    12841 atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg cctgaatacc
    12901 ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat ggcgaccaac
    12961 cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc ttgccggaat
    13021 acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac gctgcttctg
    13081 aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg
    13141 ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt
    13201 ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc cagataaaca
    13261 atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa atgcgcataa
    13321 ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt caggtagtaa
    13381 ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg catcaccggc
    13441 ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag cgtgcgctct
    13501 ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg catgttgttg
    13561 aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag cattacgcgg
    13621 gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt ttcatagcca
    13681 gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca
    13741 gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg ggaacgacct
    13801 ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt cggcatccag
    13861 tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca
    13921 atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag atcgtaaatt
    13981 ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag
    14041 gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat aacgcgaatg
    14101 acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag cacgtaaggc
    14161 atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat gtagctgtgc
    14221 caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat gtctggtacg
    14281 cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac
    14341 gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc gcgtttcatc
    14401 ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt ggctttggtc
    14461 agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag cgggtgacct
    14521 ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca
    14581 aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc
    14641 ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg gttacgatcg
    14701 atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg
    14761 cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt cggcagtttc
    14821 tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac gggaaccccg
    14881 gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt
    14941 accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt taagtcagtc
    15001 atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat
    15061 agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta aatctcgtca
    15121 ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg ctctgattgc
    15181 atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg acgttctttg
    15241 ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg cggcgggcag
    15301 cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac ggaataaata
    15361 tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt tggttccagc
    15421 atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt tcctgcaacc
    15481 accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg
    15541 tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag accgaagtta
    15601 tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt gcccataaac
    15661 acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt ttgcaggggg
    15721 taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat cttaatgcct
    15781 cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt
    15841 gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag acctgccagt
    15901 aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc gcggatagag
    15961 gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa catggccacc
    16021 agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat accggattca
    16081 aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc
    16141 atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca gtgatgagcg
    16201 atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc gccagattca
    16261 ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa aaaaacctta
    16321 caataactgt agaaattctt ttatacagct aattgatg
//
LOCUS       DAZPFP010000074        16258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPFP010000074 DAZPFP010000000
VERSION     DAZPFP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.074"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUR4_002283"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUR4_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUR4_002284"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUR4_002284"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1455857.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUR4_002285"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUR4_002285"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1455858.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUR4_002286"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUR4_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUR4_002287"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUR4_002287"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1455859.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHUR4_002288"
     CDS             complement(5877..6218)
                     /locus_tag="ACHUR4_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1455860.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUR4_002289"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUR4_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1455861.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUR4_002290"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUR4_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1455862.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUR4_002291"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUR4_002291"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1455863.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHUR4_002292"
     CDS             12897..14240
                     /locus_tag="ACHUR4_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1455864.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHUR4_002293"
     CDS             complement(14302..14508)
                     /locus_tag="ACHUR4_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1455865.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHUR4_002294"
     CDS             14833..15387
                     /locus_tag="ACHUR4_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1455866.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16258)
                     /locus_tag="ACHUR4_002295"
     CDS             complement(15450..>16258)
                     /locus_tag="ACHUR4_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455867.1"
                     /translation="SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISF
                     SLKDNGAEYTITLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKK
                     QSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEK
                     IIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAE
                     QVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3883 a         4433 c         3973 g         3969 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaa
//
LOCUS       DAZPFP010000075        16235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPFP010000075 DAZPFP010000000
VERSION     DAZPFP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.075"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..194)
                     /locus_tag="ACHUR4_002296"
     CDS             complement(<1..194)
                     /locus_tag="ACHUR4_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1455868.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRT"
     gene            544..1566
                     /locus_tag="ACHUR4_002297"
     CDS             544..1566
                     /locus_tag="ACHUR4_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR1455869.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1566..2546
                     /locus_tag="ACHUR4_002298"
     CDS             1566..2546
                     /locus_tag="ACHUR4_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR1455870.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2543..3301
                     /locus_tag="ACHUR4_002299"
     CDS             2543..3301
                     /locus_tag="ACHUR4_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1455871.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3311..4120
                     /locus_tag="ACHUR4_002300"
     CDS             3311..4120
                     /locus_tag="ACHUR4_002300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1455872.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4120..4974
                     /gene="modD"
                     /locus_tag="ACHUR4_002301"
     CDS             4120..4974
                     /gene="modD"
                     /locus_tag="ACHUR4_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR1455873.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5000..6954
                     /locus_tag="ACHUR4_002302"
                     /pseudo
     CDS             5000..6954
                     /locus_tag="ACHUR4_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(7004..7258)
                     /locus_tag="ACHUR4_002303"
     CDS             complement(7004..7258)
                     /locus_tag="ACHUR4_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1455874.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7459..8193
                     /locus_tag="ACHUR4_002304"
     CDS             7459..8193
                     /locus_tag="ACHUR4_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1455875.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8195..8806)
                     /gene="emtA"
                     /locus_tag="ACHUR4_002305"
     CDS             complement(8195..8806)
                     /gene="emtA"
                     /locus_tag="ACHUR4_002305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1455876.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8906..9820
                     /gene="ldcA"
                     /locus_tag="ACHUR4_002306"
     CDS             8906..9820
                     /gene="ldcA"
                     /locus_tag="ACHUR4_002306"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1455877.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9915..11651
                     /locus_tag="ACHUR4_002307"
     CDS             9915..11651
                     /locus_tag="ACHUR4_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1455878.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12262..13676
                     /locus_tag="ACHUR4_002308"
                     /pseudo
     CDS             12262..13676
                     /locus_tag="ACHUR4_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13844..14914)
                     /gene="dadX"
                     /locus_tag="ACHUR4_002309"
     CDS             complement(13844..14914)
                     /gene="dadX"
                     /locus_tag="ACHUR4_002309"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1455879.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14924..16222)
                     /gene="dadA"
                     /locus_tag="ACHUR4_002310"
     CDS             complement(14924..16222)
                     /gene="dadA"
                     /locus_tag="ACHUR4_002310"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1455880.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         3913 a         4314 c         4064 g         3944 t
ORIGIN      
        1 cacgtccgaa taaacgcatt cagtaaatta ctctctctaa gaaaataaat atcttcattg
       61 ttgttaatga cattaataac gtcgttctta aaatcctcaa agtcttgttg cggtaacttc
      121 tggttacgct gcttactatc agcaagcggt gaatccggac gacaacaaca taggttttgt
      181 ataaccccca gcattctttt cacctccgtt aacagtatat tcacgacact gaaacgagag
      241 aatgctgata attttctcgg cgagatatca aaaatcgctc attataaaac aagtaacttc
      301 aacgtcgcgt aaccgtggac gataaaaaaa caataatgtc attcatattt aaataattta
      361 gccttaaatg atttaaataa tattagccac ctgataaata catttttaat gatagagagg
      421 gtcaacaggc ttgcggaagt cgtcacatct tcatgttttc aatagctaaa ctttgatatt
      481 gaacatgatt tttatcgata tatatgaaag tatatatcga ttttactttt caggatgttc
      541 accatgccca taaccagacg gacgtttgcg caggcacttg catctaccct gctcctgcag
      601 tctctaccgt ccttttcaca aacagttaat cgttttgctt cgcaatctct tcccgaagcc
      661 caaaacatta cacgcatcgt cagcgcaggc gcgcccgccg atttgctctt gctggctgtc
      721 gcgccagaaa aaatggtcgg tttttcctct tttgattttg cccgtcaggc attaattcca
      781 ttgccagagc acattcgcca gttacccagg ctgggacgac tcgccgggcg cgccagcaca
      841 ctatcgctgg aagggcttat ggcgttacat cccgatttgg tcgttgattg cggcaatacg
      901 gatgaaacct tgatctccca ggcacggcag gttagcgaac agacacaaat cccctggtta
      961 ttgcttaacg gaaaactggc gcaatcagca gaacagttaa caacgcttgg caaaacgtta
     1021 ggcgaagagc accgcgccgc agaacaagcc aatctcgcca gccacttcgt tggtgaagct
     1081 caggcattcg ccacctcacc cgccgctaac ctccgctttt atgctgcgcg cggtccccga
     1141 gggctggaaa cgggcttaca gggttcgttg cataccgagg cggcggaatt attaggtttg
     1201 cacaacgtcg cgcaaatagc cgatcgccac ggcctgacac aggtttccat ggaaaatctc
     1261 ctgcgctggc agccggatat tattctggtt caggaggccg ttactgcaga ttttattcgt
     1321 cgtgatcctc tctggcaggg cgtgaaagcc gttgcggaac aacgcatctt atttttaagt
     1381 ggcttgccct ttggctggct cgatgccccg ccgggaatca accgtcttct gggattacgc
     1441 agacttcacg cctggctgga tcccgccatc aatcgccagt ttaaaagtga catgcagcat
     1501 tacgcccaac tgttctggca ttgttcactc agtgatgccg actatcaaaa attggtggcg
     1561 agctaatgag aatcgtaaac ggatgtattt tgctgctggc agctatcagt atcacttttg
     1621 ccgccgtaag tggggcttat catctcgata tgcagcaact gctggcgcta attcttcgtc
     1681 aagaaaatga gtctgtgcag gaacaaattg tcttctggca aatccgcgtg ccgcgtattc
     1741 ttgctgcgct gttactgggc gcagcgctgg caggagccgg aaccacctat cagggaatgt
     1801 tgcgtaatcc cttagtttca ccggatattt taggcgtctc cgccggtgcc ggacttggtg
     1861 cctgcgcggc aatcctgtgg ggattaccca ttgtttacat ccagctgtac gcattttgcg
     1921 gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg tcgggtgacc cgccacgatc
     1981 cgatcttaac cctggtgctg gtaggcattg cgttaggaac gttgtgcgga gcggggatat
     2041 cgctaattaa aacgctggcc gatccttaca cccagttacc gtcaattacc ttctggttgc
     2101 tggggggact gtccaccgtg accttacgcg atctctgcta tgctgcaccg atcattctca
     2161 taggctcctt gcccctgttt tttctccgct ggcgcatgaa tttactcacg ctttccgacg
     2221 acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg ctttggattg attgtctgcg
     2281 ccacactaat caccgccagc acggtagcaa tagcgggcat aattggttgg atcggtctgg
     2341 tcgttccgca cattgcccgc ttgttgaccg gtcataacca tcagcagctg ttgcctatgg
     2401 caatgtgtac tggcgctatt ctgctcctgc ttaccgatac gctggcgcgc agcattggaa
     2461 caacagaaat cccacttggc attctgaccg cttttgttgg cgcaccgttt ttcctgcttt
     2521 tgttgttacg cggaggtcgc cagtgacttt gcttgccgtt cgtcatgcgt ctctgggtta
     2581 ctcccgccat ccagtgctac gcgacgtgtc cttcaccctg tcgcaaggaa caatttgttg
     2641 tctgcttggc gcgaatggtt gcggcaaaac cacactcatg cgctccattc ttggcgttat
     2701 accgttatta aaaggcgaga tcctactgga tagcctctct gtgcagacac tcagccatcg
     2761 ccagcgcgca caggctattg cctgggtgcc gcaggcgcac gacggtattt ttgctttcag
     2821 tgtgctggat atggtgttga tgggcctggc ccccaccata ggcgcttttt ccgttcccgg
     2881 taagcaagaa cgcctgaaag ccacggagca actggaaaag ctgggcattt tgcatctggc
     2941 gacacgacgc tggaatacgc ttagcggcgg tgaacgtcag ctggctttaa tcgcccgtgc
     3001 actcgtccaa caaccgcgct tgttgttact ggatgagccc gcctccagcc tcgatttcgg
     3061 tcatcagatc caacttctgg acacgctggc gcagctcaaa aataacggca tgacaatgtt
     3121 aatgtcgact caccatacgc tccatgccaa cgccattgcc gacagcatta tccaagtaga
     3181 gccagatgga cgagtaacac aaggcttacc gaccgaacag ttaacaacga acaaactggc
     3241 ggccctgtat cgggtttctg ccgaccaaat tcatcatcac ctctctgcaa ttagccgcta
     3301 aggaacaatc atgctcattg atgatattga ttttgcagac ctttatctgc agcaactcaa
     3361 actggctcac cgcacagaaa aaacgcccga tcactgggat caacgggcgg agaaaatggc
     3421 ggaaaattgc gcctcgccaa cggacagcta cctgcaacaa ctgatcgcta aaattgacct
     3481 gcaaggcgct cagaccttat tcgatatggg ctgcggacca ggcaccgtat cgctggcgct
     3541 ggctgacaaa ctcactacgg tttatggcgt tgattacagt caggggatgc tgaacgttgc
     3601 cgcccgccgc gccgccgcac tgaaggctga caatgtccac tggatccagc gcgcctggga
     3661 agaggactgg agtgacctgc cacgctgcga tattgccgtg gcttcccgct ccactctggt
     3721 ggctgatatg cgccatgcaa tgagcaaact gaataatcag gcacggcttc gcgtctatac
     3781 aactcatctg gtcagcacct cgttcgtctc tccagccatt cagcgggcgg ctggtcggga
     3841 agtgatcgaa ttacccaact atatctttgc gctcaacgtc ctgtatcaga tgggaattta
     3901 tgcccacgtt gattttatcc gtggacaaaa ttgccaacag gataacagca cctgggaacg
     3961 ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac gacgatgagc gtgagcggtt
     4021 atatcactgg tatcaacaac aagatgctcg tgccctcgcc cctgccagcc gcgactgggc
     4081 gctcatctgg tgggatagcg taccgcagga ggcactgtga tgattttcct ttctcaggca
     4141 caaatcgatg cgttactgct ggaagatatc cagggaggtg acctgaccac gcgagcatta
     4201 aatattggac accagcctgg ctatatagcg ttttttcacc gtcagggcgg ctgcgtcagc
     4261 ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat taactattga tgacgcggtc
     4321 agcgacggtt cacaagcgaa cgcgggtcaa cggctaatcc gtgcgcaggg taatgccgca
     4381 gcacttcatc agggatggaa ggcagtccag aatgtgctgg agtggagttg cggtgtttct
     4441 gattatctcg ctcaaatgct ggcattgctt cgtgaacgtt atcctgacgg caatatcgcc
     4501 tgcacccgaa aagcaattcc gggcacccgc ttactggctt cgcaggcaat tctggctgcc
     4561 ggaggactaa ttcatcgtgc cggatgtgcg gaaaccatat tattgtttgc caaccaccgc
     4621 cattttcttc atgacaatca gggctggtca ggcgcaatcg ctcagttacg tcgccacgca
     4681 cccgagaaga aaattgttgt tgaagccgac acgccgaaag aggctatcgc cgcgttacgc
     4741 gcacaaccag acgtacttca gctcgacaaa tttagtccgc aacaggcaac tgaaattgcg
     4801 caaatagcgc cgtcgctggc tcctcactgc acgctggctc tgaccggcgg aattaatctg
     4861 acgacactca aaaattacct cgactgcggc attcgccttt ttattacctc cgcgccttat
     4921 tacgcggcac cagctgacat taaagtcagt ctgcaaccca cagcctctat ttaattcttt
     4981 gttaaacgga atttattcta tgagactcaa aaaacgttat ctctgcacag cattatcact
     5041 tgcctttacc cagcaggccg tagcggctca ggagagcgac acgctgaccg tatggtccag
     5101 tccggtatca tcgacgacga ccaccgttct cgatcaaccc accatgaagg ccttggataa
     5161 acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg gtgctgcaaa agtcaggcag
     5221 ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt cggcaggtgc cggtctattt
     5281 cgacggtgtg cccatttatg tcccctatga cggcaacctc gatctggcgc ggattctgac
     5341 caacaatctg ggggcagtcg aagtttccaa agggtattcg tcgctgcttc atgggcctaa
     5401 tcagatgggc ggagccatta atatcaccac cctgaagaag caagtctggg atatcgccag
     5461 ggatggagcc gtagtcagga caatgcctac gatatgcatg cttcatttgc cgccagcagc
     5521 gatcttgggt atttgcaagt cagcggtagc cagctaaagc aggattttct cggcctgccg
     5581 catggtgtaa ataatgatat tgcaggcaaa cacggcaaga tgattaattc atcggctgat
     5641 gataaacgcg gcattgtgaa gctcggtttt acaccgcgcg aaaacgatga atacacgctg
     5701 acttacatta agcaggatgg tgaaaaagat aacccgccat acagcggaaa ttgtggtcaa
     5761 aaatcacgct actggcagtg gccagagtat gacaaagaaa gtttttatta tcagggagcg
     5821 acccaactaa acgaccgttt taccctgaaa agtcggctgt atcgcgacac ctttgaaaat
     5881 acgctgatga tgtacaactc gctggctgat ttgaaaaata aaaaaggcag ctacagccat
     5941 tattccgatt acagcgacgg tgccggatta caactggcag ccgatgtgcg tgaaaacgat
     6001 ctgctgtcgt ttgccgttaa ctggaaagat gacgtacacc gggaaaaagg tgcgccgcac
     6061 gccgcttacg atcgctatga agatcgtacc tggtcgctcg ccagtgaata tcaatgggct
     6121 gctgctgata atgtcgatgt cgtggcggga atcagctatg actggcgcga tagcgtagaa
     6181 gcgaaaaaac acgagaaaga tggcagcatc acccattatg acgacaacaa tcagtcagct
     6241 tttaactggc aggtgatggg gaaataccac tttgccaatg aagacactct ggcgctttcg
     6301 tactatgacc gcacacgctt tccgacgctg aaagaacgct ataccacatc caaacctgcg
     6361 tataaccaga tagcgattgt taacccgcag ctcaagccgg aacgcgctcg cggggtggat
     6421 ttaacctgga atggtgcctt cacgcgcgac tggggatttg aggtcagcgt ttactataac
     6481 cgggtgagtg atgccatcct ctcgcacaat atcaatgccg ataccattca aaatcagaac
     6541 agcggcacgg tggattacag cggtctggat gccggtatta aggggaaaat cagcaatata
     6601 ctggatgtag gattgagcta cgctctgatc cacgctgacg ccaaacgcaa agacatcggc
     6661 aagataaccg atcttccaac gcagacaatg accgcatgga tgacactcaa accatgggag
     6721 ccgttaagcg taacgctgtc ggaagaggcg cgttcctcca gctacagcaa cagtgacggt
     6781 tcacaaaaag ccgccggttt tgcggtgacc cacattcgag ccgattacac cttaggtcat
     6841 ggcttcagcg tcaatgcgtc ggtcaataac ctgtttgata cccaatatgc ctacagtgaa
     6901 gggtttattg aagaaggtcg caatttctgg gcaggtgtcg agtatacgtt ctgatgtgaa
     6961 agcggatagc ggcatctgta tcgctatccg ccttatgaaa atcttattct cttcgcagga
     7021 ggcggaatac gcccagcacc aggatagcgc ccaccaccgc caccaggaag ctgtgcaaat
     7081 taaaaccgct gatggagccg tcaatgccaa acatggtcgc cagccagccg ccgaccaccg
     7141 caccgactat cccgagaata caggtcagga aaaatccacc accatcacgc cccggcatga
     7201 ttagcttgcc gataatgccg gctatcaggc caaaaataat ccaggcaata attcccatgt
     7261 tcaggccctc gtttcgatga atcggtcgca agaaatgctt acgtcaacgc tctaagtata
     7321 ggcaagggcg ggatcgatgc tgggcgtttg tcggctgatt taagaacaac atgtaaaaaa
     7381 tattcacgca tttgtattaa gttttgttaa ctatgaccga taaaccaaag acagtttgtc
     7441 agtcaggagt ttttccgcgt gagtcattac catgagcagt tcctgaaaca aaatccgtta
     7501 gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa ttcctttgcg tctcagttgg
     7561 aatggcgggc agctgatcag caaaatactg gcaataaccc cggataaact ggtgctggat
     7621 ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg cacagcacat taccattacc
     7681 gccgaaactc agggtgcgaa agtcgagttt actgttgaac aactacagca gagtgaatac
     7741 ttgcagcttc cggcatttat taccgtaccg cctcccacct tatggtttgt acaacgacgc
     7801 cgatatttcc gcatctccgc cccactccat ccgccttatt tttgccagac caaactggcg
     7861 gataacagta cgttacgttt ccgcttgtat gatttgtcgt taggcggcat gggcgcatta
     7921 ctggaaacag caaagcctgc cgaattacaa gaaggtatgc gcttcgctca aattgaagtc
     7981 aacatggggc aatggggtgt ttttcacttt gacgcccagt taatctccac cagcgagcgc
     8041 aaagtgattg atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt
     8101 aacgtcagcc cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgagaa
     8161 gcccgggaaa aagcggacaa agtgcgcgac tgatttacat cgcgtccagt gcctgctcaa
     8221 gtttgtagat atagcgcgga gcctgcggcg caggatgatt tcgcgctacg tgatcgagga
     8281 actcgtcagc atccaaatcg ttgattttgc tgatcgcctt tttccgatct gacgagaaag
     8341 tccgtagcag cgcacctgcc ccgttagcgt atgacaccac cagcgcatat tgcagtacct
     8401 tcggatcttc aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca
     8461 ttgaaatatt acgctctgga tttttcagct cgctggtcgt cggctcacca ctccagccca
     8521 tacggcgata aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat
     8581 tcgatttact caccgcgttc ggattaccac ccgattcgat agcgataatc gccgtgatca
     8641 attgtggatc gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg
     8701 cacgttgcac cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac
     8761 aacccgctaa taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta
     8821 tagcctggaa tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg
     8881 tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta
     8941 cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg cggggcatca
     9001 agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga
     9061 gcgtctggaa gatctcaatt cccttgccag actgactacc cccaacacca tcgtgctggc
     9121 tgtacgcggc ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt
     9181 ggctcgccaa caacatgacc cgttactcat ttgcggacat agcgatttta ccgccattca
     9241 gtgcggtctt ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa
     9301 ctttggtgcg gatgaactga acgcctttac cgagcaccat ttctggctgg cgttacgcaa
     9361 tgaaaccttc accattgaat ggcaagggga aggcccgaca tgtcagaccg aaggcacgtt
     9421 gtggggaggc aatcttgcga tgctgatttc actgattggt acaccgtgga tgccaaaaat
     9481 tgagaacggt attctggtgc tggaagatat taacgtgcac cctttccgcg tcgaacgtat
     9541 gctgttgcag ctctatcatg cgggaatttt gccacgtcag aaggcgatta ttctcggtag
     9601 ctttagcggc agcacgccca atgattatga cgcaggttac aacctggagt cagtgtacgc
     9661 gtttttgcgt tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca
     9721 acgcacggtt acgctgccgt tgggcgcaca tgccattctg aataacaccc gggaaggcgc
     9781 tcagttgacg attagcggtc atcctgttct taaaatgtaa aaaaatcacg cttcagggct
     9841 aagtaaaacg ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca
     9901 ggagtaaccg accattggat gccacaacaa taattagcct tttcatctta ggatccatcc
     9961 ttgtcaccag cagtatatta cttagttcat tttcttcccg tcttggcatt cctattctgg
    10021 ttatttttct ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt atcccgtttg
    10081 ataattaccc cttcgcctac atggtaagta acctggcgct ggcgattatt ttgctcgacg
    10141 gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt aggaccggcg ctgtcgctgg
    10201 cgacgctggg cgtacttatc acctctggtt taaccggcat gatggcggcg tggctgttta
    10261 atcttgattt gattgaaggc ttattaatcg gcgctatcgt cggctccacc gatgctgcag
    10321 cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa
    10381 ttgaatccgg cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcaatga
    10441 tccagcagca tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg
    10501 gcctcgggat tgtcatcggt cttggcggtg gttatttact gctgcaaatg attaatcgca
    10561 tcgccctgcc tgccggatta tatccattgc tggcattaag cggcggtatt ttaatttttg
    10621 ctttaaccac ggcgctggaa ggcagcggta ttctggctgt ttatctgtgc ggttttctgc
    10681 tgggtaatcg gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct
    10741 ggctggcgca aatcgccatg ttcctggtgc tggggctatt ggttaaccca agcgatctgc
    10801 tgcctattgc cattccggcg ctcattttgt cggcatggat gatattcttc gcccgtcccc
    10861 tttcggtatt tgctggattg ctccctttcc gcggtttcaa tctgcgtgag cgcgtgttta
    10921 tcagctgggt gggattacgc ggcgcggtgc caatcatcct agccgtgttc ccgatgatgg
    10981 cggggctgga gaatgcgcga ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac
    11041 tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc
    11101 ccgtcggacg tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc
    11161 agtttgttta tcaattgagt gctgataaat ggtgtgtagg tgcggcactg cgcgatttgc
    11221 atatgccaaa agagacgcgt attgcagccc tgtttcgtga taaccagttg cttcatccta
    11281 ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt aattggtcgg gaacgcgatc
    11341 tcccggcgct cggtaaactg ttcagccagt cgccaccggt cgcgctggat caacgcttct
    11401 ttggtgactt cattctcgaa gccagcgcca aatatgctga tgttgcgctg atatatggcc
    11461 tggaagacgg gcgagaatat cgtgataaac agcaaacgct gggtgaaatt gtccagcagt
    11521 tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg
    11581 tggccgagaa agaagataat gaagtgttga agattggcgt tcgggtggct gaagaggaag
    11641 ctgaatctta aatttaatgc agcatctatc gccggatgcg atgctttact gaacgcagcc
    11701 tgagcctgta ataaacttgt catttcctga ttttgatatg ctcatcccaa catcagaaga
    11761 aggttaaatt atggatgaca aaccattgca ggctctggct aatgaactca aaacaccggc
    11821 tcaagaaagt cagcgtaagg gctttttccg actgtaacag gtaataaaaa ccagccccgc
    11881 attaccggtc aaccatcagg ggcccctgcc tgacatcgcc tgaacatatt cccccacgca
    11941 acctgccgca ctgacatagc ccacacaata ggcgtttcat ccgcctgtag caccggatgc
    12001 atgagcgatt tcttatagtt attcctttgc ttaataacga ttatttccgg gtcgttcact
    12061 ctcgtatttt ctttaatacg tcagagctac ctaggccgta atacagcatg taccagaccc
    12121 gacacggcat ttctgaccag gaaaaaagtc aacgatgagt agtgatatca gcgcaaatga
    12181 cgtcctgtgg ctcctttgcg gtgaccacat tcacgtctcc ggcgctgcca gaggatattt
    12241 atttcaagga aacgtagcat tatggctagc tttaaagaca tctgcccatt cgcccccgcc
    12301 attcccccat cttccgttaa cccaggcgta cgacgcccgc cagggcggct cttctcaaga
    12361 atcaaatttt gcagaacatg aagtatctca gcccgcaaaa attcagctgg ggtgatgccc
    12421 cctggcagat cattgacctc agtatcgcgg gcaaagtgaa catacaggtg gacaacaata
    12481 ccatcatcac gttggggact cgtctgaatc agcaacataa cgagttcatg atggtcgcca
    12541 aatggtgcga atgggctata cagcaggatg gtttgcaaga aaacctgcaa aaaaacctgt
    12601 acgaaatcct ggaagaaaac cagcaaaaca aacagtcaga aattccccaa gaagacctga
    12661 aagaaagtct ggaagaaatc aaggaaaata tcctggaaga aaacctgcca gcaagccgga
    12721 ttgaaaaccg ggcagaagca ttgcgcagaa tgaaggaatg tctcattaca agacggagta
    12781 tgctcaatct tagcaacctg ggattgactt cactccctga gaatttgcct ccacatctga
    12841 ttgaatttta ctgcagtaaa aacgtgttga ccgcgttacc gaaggtgatg ccaaagtggc
    12901 tgctggtgct tgactgtaca gataacgtct tgatcttgtt accgaaggtg cagccctcaa
    12961 aactgatggt actgaagtgc tatgaaaaca gtattatctg gctgcctgag ctgtcgacta
    13021 acctgagagt gattaactgt tctgaaaact tcttgcaatt tttaccgccg tcgatgcccc
    13081 agtacctgta taaactatcc tgtgccggca acaatattaa ctcaatacct gatgagatgc
    13141 tggagaactt gactcgcctg aaagtatttg actgttctag taacgatttg atctcttcac
    13201 cacggctgcc gcccaaacta ataatttact actgtggaga aaacaagttt aaaacggtac
    13261 aggtgccgca gccccagagc ctgaaggtgt ttgactgtaa tggtaacccg tgggacaaag
    13321 acaatttacc gacgctgctc aaagccgtcg agggcctgaa aaaacagcag ggcctgaagg
    13381 accttttgga ctttttgcac aaggaaggct aggttgacct ggaaggactc gaggagctgg
    13441 aggaccttat ggatctggag ttcctggatg acccggaact cctggagcgc gtgaaggtac
    13501 aggaggacct ggagctcctg gatcaacagt tgggcctgtt gagtctggaa agacaacagg
    13561 actcgcagcc tgttaatcaa caatctgaac atgaacccga atctgcatca agggtgaagc
    13621 gtgatttatc tgaagtcgac tccgagtcaa caatgaagcg taagcgtttt atgtagaacg
    13681 cctgtgcggc gagttcaggt tccggggaaa tagtgtccat tcttttaatg gacactatca
    13741 tgggcaatgg ctctgtcaca ttaacaacta cagttgctga ccagccggat ggatgatttt
    13801 ttcgcaacca gaagacgctg cctccgatcc ggcttacaac aagttacact gtcacaaccg
    13861 ggacgcgtaa cgccagtgcg cacatcaact catagcccac cgttccggca gcggcggcga
    13921 catcatcaat tttgatctcc ttgccccaca gctcaaccgg cgtaccaatc cccgcctgcg
    13981 ggcaaggcgt taaatcaacc gccagcatat ccatcgagac ggtccccacc gtcatggtgc
    14041 gcacgccgtc cactaaaaca ggagcaccgg taggcgcatg gcgcggataa ccgtcggcgt
    14101 atcctgcggc gacaatgccg attcgctgtt catcgcgcgc agtatagcga ccgccgtagc
    14161 ccacacgctc gcccgctttt agcgtctgga caccaataat ctcactgctt agcgtcatca
    14221 ccggacgtaa tccggtattg gcgatatcac gccactgacc ggacggcgaa gcgccataca
    14281 aaataatgcc aggccgaacc cagtcaaaat gcgcttccgg gtgccacaga gtcgccgccg
    14341 aattggacaa cgaacgccga cactccagcc cctccgccgc ctgctcaata cgcgccatcg
    14401 cgccggaaat tccatcagga tgttccgctt cagcaaaatg cgacatcagg gtcatttcgc
    14461 caacattcgc cattgcccgc aactgctgcc agacggtaag cacgcgatcg gactggaagc
    14521 ccaaccgatt catcccactg ttcactttaa gataaatatc caacggtgct tttagccgcg
    14581 cattttgcag tgctttgagc tgccagttgc tgtgtacgca ggtggtcagg cggtgctggt
    14641 cataaatctc cagatcctga gcatggaaaa atccttccag catcaggatc ggccccttcc
    14701 agccgcgctc acgtaacgtt attgcctctt ccaggttaag taatgcaaag ccatcggtgg
    14761 ccccgagcgc gctccagata cgctcaatac catgcccgta agcgttcgct tttaccaccg
    14821 accagacgcg cgcgtgcggc gcggcctggc ggacaatgga cagattctgt tttaatgcct
    14881 gcagatcgag gctggcctgt atcggacggg tcatctcgtt tccttagctg tgtgcgccat
    14941 gtaaatggcc cggacgtgat ggcgtaaatc cacggctgta gcgcgctacg cttagatcct
    15001 catatgggat cgctggcgtg cgaccagaga gcagatcgct taacaactga ccggaaccgc
    15061 aagccatcgt ccagccgagc gtgccgtgac cggtattcag ccacagattt ttaaagcgtg
    15121 tactcccgac aaccggcgta ccgtctggcg tcatcgggcg caaacccgtc cagaaagtcg
    15181 cctgctcgac atgaccgccg cgtggataga gatcgcgaac caccatttcc agcgtttcac
    15241 gacgcggttg caacagctcg gtgttaaaac caacaatttc cgccattccg ccaacacgaa
    15301 tgcggttatc gaaacgggta atggcgattt tgtaggtttc atcaagaatg gtggatactg
    15361 gcgcaccatc ttcctgcgca attggaatag tcagcgagta gcctttcagc ggatacaccg
    15421 gaatatcaac aatgcctttg agcatcgccg tcgagtaaga accaaacgcc atcacatacg
    15481 catcggcctt aatcacttca tcgccaaact tcacgccgta gatttgctcg ccgtcgcaaa
    15541 gcagttggtc aacgggcgta ttaaagcgga atttaacccc cgcctgctct gccatccgcg
    15601 ccagattctg ggtaaacagc tggcagtctc cggtttcatc attgggtaac tgcaggccgc
    15661 ccgtcagttt gtgcgctact tctgccagcg cgggatccac ttccgccagg cggctggatt
    15721 ccagcagctg atacggtacg ccggcatctt ccagcacggc gatatcgcgg gtcgcatttt
    15781 catactgttg ttcggtacgg aacagttgca gcgtcccacc ctgacgccct tcatactgaa
    15841 tattggtttc ggcgcgcaat gctttcaggc aatcacggct gtattccgcc agacgcacca
    15901 tccgcccttt gttttccatg tagtggctgg tgtcgcagtt acgtaacatt tgccacatcc
    15961 atttcaactg aaactgcgta ccgtcgagac gaaccgccag cggcgcatgg cgctggaaca
    16021 tccatttaat cgcttttaaa ggcacacctg gtgctgccca cggtgcagca tatccggggg
    16081 agatttgccc ggcattcgcg gcactggttt ccagggcggc ccccggctcc cgatcaatga
    16141 cggtgacctc atgtcctgcc tgatttaagt accaggcgct ggcaacgcct accacaccac
    16201 ttcccagtat gacaactcgc atagccactc cgata
//
LOCUS       DAZPFP010000076        16139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPFP010000076 DAZPFP010000000
VERSION     DAZPFP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.076"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHUR4_002311"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHUR4_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHUR4_002312"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHUR4_002312"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1455881.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHUR4_002313"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHUR4_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1455882.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUR4_002314"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUR4_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1455883.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUR4_002315"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUR4_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1455884.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUR4_002316"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUR4_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1455885.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUR4_002317"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUR4_002317"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1455886.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUR4_002318"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUR4_002318"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1455887.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUR4_002319"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUR4_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1455888.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUR4_002320"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUR4_002320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1455889.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUR4_002321"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUR4_002321"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1455890.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUR4_002322"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUR4_002322"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1455891.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHUR4_002323"
     CDS             10748..11212
                     /locus_tag="ACHUR4_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1455892.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUR4_002324"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUR4_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1455893.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHUR4_002325"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHUR4_002325"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1455894.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUR4_002326"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUR4_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1455895.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUR4_002327"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUR4_002327"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1455896.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUR4_002328"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUR4_002328"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1455897.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4069 a         3766 c         4273 g         4031 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcatttta
//
LOCUS       DAZPFP010000077        16052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPFP010000077 DAZPFP010000000
VERSION     DAZPFP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.077"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..675
                     /locus_tag="ACHUR4_002329"
     CDS             <1..675
                     /locus_tag="ACHUR4_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1455898.1"
                     /translation="LSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQ
                     TIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGG
                     IKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHYFSAYSLGRLY
                     WQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTL
                     KDVSDL"
     gene            672..2123
                     /gene="djlC"
                     /locus_tag="ACHUR4_002330"
     CDS             672..2123
                     /gene="djlC"
                     /locus_tag="ACHUR4_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1455899.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEEESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2183..3853)
                     /gene="hscC"
                     /locus_tag="ACHUR4_002331"
                     /pseudo
     CDS             complement(2183..3853)
                     /gene="hscC"
                     /locus_tag="ACHUR4_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(3937..4872)
                     /gene="rihA"
                     /locus_tag="ACHUR4_002332"
     CDS             complement(3937..4872)
                     /gene="rihA"
                     /locus_tag="ACHUR4_002332"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1455900.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4990..5715)
                     /gene="gltL"
                     /locus_tag="ACHUR4_002333"
     CDS             complement(4990..5715)
                     /gene="gltL"
                     /locus_tag="ACHUR4_002333"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1455901.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5715..6389)
                     /gene="gltK"
                     /locus_tag="ACHUR4_002334"
     CDS             complement(5715..6389)
                     /gene="gltK"
                     /locus_tag="ACHUR4_002334"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1455902.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6389..7129)
                     /gene="gltJ"
                     /locus_tag="ACHUR4_002335"
     CDS             complement(6389..7129)
                     /gene="gltJ"
                     /locus_tag="ACHUR4_002335"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1455903.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7299..8207)
                     /gene="gltI"
                     /locus_tag="ACHUR4_002336"
     CDS             complement(7299..8207)
                     /gene="gltI"
                     /locus_tag="ACHUR4_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1455904.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8604..10142)
                     /gene="lnt"
                     /locus_tag="ACHUR4_002337"
     CDS             complement(8604..10142)
                     /gene="lnt"
                     /locus_tag="ACHUR4_002337"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1455905.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10167..11045)
                     /gene="corC"
                     /locus_tag="ACHUR4_002338"
     CDS             complement(10167..11045)
                     /gene="corC"
                     /locus_tag="ACHUR4_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1455906.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11135..11602)
                     /gene="ybeY"
                     /locus_tag="ACHUR4_002339"
     CDS             complement(11135..11602)
                     /gene="ybeY"
                     /locus_tag="ACHUR4_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1455907.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11599..12639)
                     /locus_tag="ACHUR4_002340"
     CDS             complement(11599..12639)
                     /locus_tag="ACHUR4_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1455908.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12792..14216)
                     /gene="miaB"
                     /locus_tag="ACHUR4_002341"
     CDS             complement(12792..14216)
                     /gene="miaB"
                     /locus_tag="ACHUR4_002341"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1455909.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14362..15537
                     /gene="ubiF"
                     /locus_tag="ACHUR4_002342"
     CDS             14362..15537
                     /gene="ubiF"
                     /locus_tag="ACHUR4_002342"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1455910.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15691..15765)
                     /locus_tag="ACHUR4_002343"
     tRNA            complement(15691..15765)
                     /locus_tag="ACHUR4_002343"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15731..15733),aa:Gln,seq:ctg)
     gene            complement(15803..15877)
                     /locus_tag="ACHUR4_002344"
     tRNA            complement(15803..15877)
                     /locus_tag="ACHUR4_002344"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15843..15845),aa:Gln,seq:ctg)
     gene            complement(15926..16002)
                     /locus_tag="ACHUR4_002345"
     tRNA            complement(15926..16002)
                     /locus_tag="ACHUR4_002345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15966..15968),aa:Met,seq:cat)
BASE COUNT         3898 a         4102 c         4169 g         3883 t
ORIGIN      
        1 ctttctgcgc ccatggaaat acttaatcag agttgcaaac ccgcgcatga ctcgccaaaa
       61 atgtataccg ggataaaaga attcgatctc tcatcatcct ggggaataaa taatcgtgat
      121 gatttaattc agactattta tcagatgact gatgatggtc atgccaatga tttagctggc
      181 ttatatctga catggcaacg ctcttcccct gaagagtgga aagcattaat tgctggtggc
      241 tcggagagag ggttgattta tacccaattc gttgcccaaa ccgctatgtg ctgtggagaa
      301 gggggaatca aagcctggga ttatgttcga atgggatttc tgtcacgggc cggtgtgctc
      361 aacaactggc tgacggaaga agagagttta tggctgcaat cgcgcgtcta tgcacgagct
      421 caccattact atcacagctg gatgcactat ttttctgcct attcactagg gcgtctctac
      481 tggcaatctt ctcagtgcga agacaacgct tcgctacgcg aggcgctaac cctgtacaaa
      541 tacgatagcg ccgggagtcg gatgttcgaa gagctggcag cgggaagcga ccgtttctat
      601 gccacgcttc cctggcagcc attaactgtc cagcctgaat gcccggtaac gcttaaggat
      661 gtgagcgacc tatgaaaaca tgttggcaaa tcctcgaaat tgaaagcacg acgcaaatag
      721 acattatccg ccaggcttat cttgctcgct taccgttgtg tcatcccgaa accgatccgc
      781 aggggtttaa agcattacgc caggcctatg aagaggcgct gcgactggcg gtaaatcctg
      841 tcgaggaagc agatgatgaa gaaaaagatg ccaccgctga acatgaaata ctacgtgcat
      901 tcaggacatt actggattca gaaagtgatc gttttcagcc ctccgcctgg cagaaattta
      961 ttcagcaatt aaatacctgg aacatggagg atgtcgatca attacgctgg ccgctgtgtg
     1021 caatcgctat agaagcgcga tatctgtcat taaattgtgc ttctttgttg gcagagcgtt
     1081 tgaactggca ttcgtttaat gacagcgaag gaatggatga ggaagaaagg gaggcttttc
     1141 ttgaggccat tcaggctggt gattgtttcg atttccttag ccttctggaa tatcccgtcg
     1201 cgttacagaa ccagactgtt gaatattact tcgcgctgga acgttgctgc cgttaccatc
     1261 ctgactatgt cactgcgttt ttggcgatgg aaggaccgtg gttcattcct gatgatgcaa
     1321 agttacatcg caaactgttg cgctggtaca gctcggtgca aacaggtatg gtggaactca
     1381 ttcctgtagc caaacagtgg caagcggaag aaccagaaag cgaagatgcc cggtattacc
     1441 agtgtgcaca acgtttgtac tgcggcgagg aggaaagcct gcttgctgat ctcggcgcgt
     1501 actgggaaag ttacccatct acacaagctg ataatttgtt gttgcagtgg agcaagcgtc
     1561 attgcccgga ttatttcgcg ttattagtga tggttatcga agcgcggagc atggtagatg
     1621 cgcaaggtca accgctgaaa tatgttcctg gtgagagcgc ccggacgcgg ctgttatggg
     1681 cggaaatttt acatagcgga aaattatcgc cgttaggtca atcgtttatt gagtcgttat
     1741 tcttcaaacg caaagcatgg gcgtggtgga aatcgagagt cggtagcgag acagaagaag
     1801 attcaccgtt actggatttg tatcgggtag cggaacaggt agtacttgaa gcgtttccga
     1861 aacaagagat gctggcccgt cttaatacaa ggctggaagg cggagatgct catccattag
     1921 aggccattat cacccggatg ctgttgacga aagtaaaact cgagccggag gatgaagatg
     1981 tcgatgagcc aacacctgaa aaccatgaag aaaaaaatga tgagggtgaa aaaccacaga
     2041 gcattaccag cattatcaaa atcagtttaa cggtgctggt tataggttat gctctcggca
     2101 aaatcgcgat gttgtttagt tgacgatgtg ttttttgcgc cgatgccgga tgcagcattt
     2161 acgccgcatc cggcagccgt gattatgagg tctcaatggc taaataaacg ctcgcccgcc
     2221 gcctgacctc atcgacgcgg gccatatcgt ttgactgcag cgccgcatca aaatcggtga
     2281 tcacccggcc aatctcttct cgctcgtcac ccagcgcccg cgcccacaac tcttccagtt
     2341 tggctttaaa ggtgcgattg atgagcatat cgcgcggata aattttcaat gcggataacc
     2401 gcgtccgact ctcttcaatc tgctgcgcgc tcaatgttac cgggctgtgg ttaatcactc
     2461 tggacttaac gctgccgtct tccagaagca cgtcaacttc aagcaacccg ttgatatcat
     2521 aactaaagcg aatatcaatc gactgataag ccccggtttt cttcaacggc acatcgaagg
     2581 attccaccag aatgttgttt ttaactttgt ggttttctcc ctgatagacg ttaaccgtaa
     2641 ttgaatcctg ttccgggtgc atggttgaat aagtttctac acgcgacacg ggcacagtgg
     2701 tgtttcgttc aataatcggc gagaaaatgc cggaaacgcc ctggcggtta acctcaacgc
     2761 ccaacgagta agggcaaata tcagtgagga tcacttcttc aatatcttca ctgcgtaagc
     2821 ggcaggcggc ctggattgct gcgcccagcg cgacaatggt gctcggatcg taactttgat
     2881 acggtaattt gccaaacaga cgcacggcga ttcgctgcac cagcggcatt tgtgacgcgc
     2941 cgccaaccag caccagactg tcgatttgac tcggcttcag acgcgcatcg cgcaacgcct
     3001 gttcaatcgg cactcgcaag cgattgagca gcggcaacca caaatcttcc agttcgttct
     3061 cgtaaaattc gcattcccgc gtttcatcct ggtactacca gcgaatgtgc attggcgatt
     3121 gattgctaca ttttgccgct tccacacagg cgtacaaggc tgccagttca ctctcgttaa
     3181 gcgtggtcct tgtgacatcc gcgcgcttta aaacgtcatc gaccaacata tgggtaaaat
     3241 cttcgccacc gaggaagttg tcgccagcgg aggcgtgaac ttcaatcacc ggcgtggcgt
     3301 actcaagcac cgtaacgtca aacgtgccgc caccgagatc aaacaccagc gaacgggtat
     3361 tttgttgggt atgcaggcca tacgccatcg cagctgctgt gggttcatta attaagcgta
     3421 ccgcatttaa cccggctaag tccgctgcta aacgggtatg cttgcgttgt tcatcgctga
     3481 aataagccag aacggagatc accacatctt taatcggacg ttgcagaaat tcttcggcat
     3541 cttcttttaa tgagcgtaat accaaagagg acagttctgg cgcgttaaat gtgtcgctgc
     3601 ctaaccgcca gttggtatta ctgcccattg cacgtttaaa taacgctgcc gttttatccg
     3661 gatgcgaagt acgccgtgat acagccggtt ttccgactaa aatatgatta ttttcatcca
     3721 tgctaattat ggatggtgtt aaatattcac cgaacttatt tggaattaat tgcgcggcac
     3781 cgtctttcca gacggcaatt aaactattgg tagtgccgag atcgatacct atggcgagtt
     3841 ctgcattatc cattgcattt atatcttgaa agaaaaagag ataaatagat taagaccacc
     3901 caggtaacgc gtggtgatct taatcaatga cgagtgttaa gcgtaaaatt tcagacgatc
     3961 agccagtaaa tcaacaaagc cctgacgatc aacatcgaca attacagtgg cattcggttt
     4021 attgccagtc agataataat aatcaacaac cgtcataccc tgggtatatt tcccctgtgt
     4081 ttccacgcca acccagcgct caacagtggt aaacaactcg ggtttcaaca gccaggcgat
     4141 ggtgcatggg tcatgcagcg gtgcgccgac aaagccccat ttttcgtctt tatgatattc
     4201 gaggaagaaa tccagcagtt cggcaacaat ggttgaaaca gggttgccaa tcgcgcggaa
     4261 acgctcggtg tcttcaacgt ggatttgcgc tttatgagta acatccagac cggccatcac
     4321 caccgggatc cctgactgga agacaatttc tgccgcttcc gggtcaacgt aaatattaaa
     4381 ttcagccgca ggcgtccagt taccaagccc cattgcgccg cccataatca cgatacgggc
     4441 aattttgcta tgcagttccg gatggctatt gagcagcaag gcaacgttag tttgcggtcc
     4501 ggtagaaaca atcgtgacag gttccgcact ttcgcgcagc gttttcgcca tcagctccac
     4561 cgccgtacag ttttgcggtg cgaacgtcgg ttccggtaat gccgggccgt cgagaccgct
     4621 ttcgccgtgc acattgtccg cgataatcaa ctcacgcatt aacggtttta ccgcgccgcc
     4681 tgccaccgga atatcggtgc gattaagcaa ggtcagcata cgcagaacat tgcgtaaggt
     4741 tttttctggt gtctggtttc cggcggaaga cgtaattgct ttgacatcaa gctctggtga
     4801 ggcgagtgcg agaactattg cgatagcgtc atcatgacct gggtcgcaat ctaacagaat
     4861 tggcagtgcc attgttgctc cttgttgtgt gcttctttgc gacaagggta acgccaggat
     4921 gtaacagata cgaggggcga aacgataaag cgtgagatgg cgcgcaattg ggtatgtgcg
     4981 ccagagtgat taatgcagga ttttcgcgag gaagtctttt gcgcggtccg atttcggatc
     5041 atcgaagaaa gcgtctttcg gcgagtcttc gacaatttta ccctcgtcca taaagatcac
     5101 ccgattcgcc actttgcggg caaagcccat ttcgtgggtc accaccatca tggtcattcc
     5161 ttcgttcgcc agttccacca tcacgtccag cacttcgttg atcatctccg gatccagcgc
     5221 cgatgtcggt tcgtcgaaca gcatcgcaat aggagccata cacaatgcgc gagcgattgc
     5281 cacgcgctgc tgctgaccgc cggaaagctg cgccggaaat ttattggcat gtgcggaaag
     5341 tcccacgcgc tccagcaatt tcaatgcttt ttcacgagcg ggcgctttat cgcgtttaag
     5401 cactttcacc tgcgccaggg tcaggttttc gataatcgac agatgaggga acagctcgaa
     5461 atgctggaat accatcccga cgcgggagcg cagctttgcc agatcggttt tcttgtcgtt
     5521 aaccacgata ccatcgacgg tgatttcacc ttgctgcact ggttcgaggc cgttgacggt
     5581 tttaatcagc gttgatttgc cggaaccaga cgggccgcaa accaccacca cttcgccttt
     5641 tttcacttcg gttgagcagt cggtcagcac ctgaaagtga ccataccatt ttgaaacatt
     5701 tttcagggta atcattatgc tgtccttctt ttcaagtagc tgaccaacaa cgacgcgcta
     5761 aggctaataa cgaaataaac aaatccggca aacaggatca tctcgacctg cgtaccatca
     5821 cgctcaccaa tggttgaggc ggtacggaag aaatcggcca gagataacac atacaccagc
     5881 gaggtatcct ggaacagtac gatgccctga gtgagcagca gcggcaccat cgcgcggaaa
     5941 gcctgcggca gaataatcag tttcatcgac tgccagtgag tcatccccaa cgccagcgcg
     6001 gcgctcgact gaccacggga aatactttga ataccggcac ggataatctc tgaatagtag
     6061 gccgcttcaa acatcgaaaa cgccaccatc gccgaaatta aacgaatatc attttttggc
     6121 gataatccca gcacattttg cagaaatccc ggcacaatca agtaaaacca cagcaaaacc
     6181 atgactaaag gaatcgagcg gaatacgtta acgtaggctt tggcaaacca cgaaacaggc
     6241 gcgaagctgg acaagcgcat caccgccagc atggtacccc acaaaatacc aatcacgact
     6301 gccgtgacgg tgattttcag ggtgatcacc agcccgtcga gcagatatgg cagggaagga
     6361 acaatggaac tccagtcaaa ctcgtacatt atttgccccc catgttgccg ggcaggcgaa
     6421 ctttacgttc aaccagcgtc atcaccagca tgataaaagc gttaatcaac acatacgcaa
     6481 gcgtgatggc ggtaaacgac tcccaggcgt gagccgagta gtcgagcaat ttacccgcct
     6541 gcgccgccat atccaccaga ccgatggtcg aggcgatggc ggagtttttc accaggttca
     6601 tcatctcgga agtcatcggc gggacgataa cgcgataagc attaggcagc agtacgtatc
     6661 gataagtctg cggtagcgtc aggcccatcg ccagcgcggc attcttctgc ccacgcggta
     6721 gcgactgaat agcggcgcgt acctgttcgc aaacacgggc ggcggtaaac agccccaggc
     6781 agagcatgga tgaaaggaaa aactgaatat tgggatccag ctcggcctta aaccacatgc
     6841 cgattttctc cggcagcagc tccgggatca ccagatacca ggtaaagaat tgcacaatca
     6901 acggcacgtt gcggaacagt tcgacataca acgtacccag accggagaga aaacggtttg
     6961 gaacggtacg taaaatgccg aaaaatgaac cgacgaggaa agcgataatc caggcgcaga
     7021 tcgacaaagc gatcgtcacc tgaaaaccac tccagaccca accaagatag gtggtattgc
     7081 cgaacggggc ttgttgtaaa aaaatacccc agttccagtc tatagacata aatctactcc
     7141 agaaaaaaga gggtagcagc gttaactgct accctcgaag attgttaccc agcgtattgc
     7201 ggttttcagg cccgatgggg aacgaccatc aggcgtatag tctgtccgtg ctacgtaaca
     7261 atcgagaggg ctggaatttc cgcccctggt tcttgtaatt agttcagtgc cttgtcattc
     7321 ggttctttga acagtgcttt catttcgtct gacagttcga aattcatgtt caggtttttc
     7381 ggcggaattg gatttttgaa ccatttatca aaccattttt ccgcttcacc ggaggtctgc
     7441 acctgagcga tagtgtcatc catcagcttt ttgaactgcg gatcgtcttt acgcaacatg
     7501 caaccgtagg cttcctgaga ctgcggcttg ccgacgatat cccagttgtc tggtttcttc
     7561 gctttcgcac gttcaccggc cagcagagcg tcatccatca taaaggcaac ggcacgaccg
     7621 ctttccaggg tgcggaaaga gtcaccgtga tctttggcgc tgatgatgcg catattcatt
     7681 ttttgctctt cattcagttt gttgagcaaa acttcagagg tagtgccgga agtgacgact
     7741 acggctttgc ctttcaggtc ggcaaaatct ttgatatcgc cgcccttttt ggtcaacagg
     7801 cgcgtaccga ccacgaaaat agtgtcagag aaagccgcct gtttttggcg ttcgacgttg
     7861 ttggtggtag aaccacattc aaaatcgaaa gtgccgtttt gcagcagtgg aatacgattt
     7921 tgtgaggtaa tcggaatcag ttttacctgc aagtccggtt tgttgagttt ctttttcact
     7981 gcttcaacaa tggcgttgga gtaatcctgc gagtaaccca ccactttttg ctgattgtcg
     8041 taataagaga aaggtactga agattcacgg tgaccgacga caatcacacc gtttttggcg
     8101 attttgtcca gcgtactgcc tgctgccggg gcggcgtcat ctgcctgtgc cagtcctgcg
     8161 gaaagcgcca gggcgaggat tgctgtggca ggtttacgta attgcatatc caacaccttt
     8221 atcttctgcg ttaagaacgc atggataccc gttgtgagtg tttgtgttgt tatcgtctgc
     8281 aactttattg tgcagtgttg tgcctgttag gtaacattta gtttggctaa atgtaaagat
     8341 attgatgttt tattgtttgt ttttgcgagg tgcaccgcac cattctgaag caaaattctt
     8401 aaaatgcact cttttggtgc taccggtgga ttgctgtggt gcaaccgggt tgtactgatg
     8461 ctggttcaag ctttgccttg tataacaaag caatagatgt gccaaagttg gataggagaa
     8521 tattgttatc cggataatgc actgatgccg catccggtga gcgtgccgaa atatgggatg
     8581 tattccggca cgataagaag ggattattta cgtcgctgac gcagactcat caacacagca
     8641 gcaaaaccaa acagtgccgt cagcacccac agcggccagt tgccggtacg tgcgtatggt
     8701 gtgagtccgg tggtcggcgt cacgttagtg gttaacacct cgcgggcgaa ctgcgggatc
     8761 atcgcctgaa tctcaccctg cgggccaatc accgccgtaa tgccgttgtt ggtgctgcgc
     8821 aacagtgggc gcgccagctc cagcgcacgc attcgcgcca tctggaagtg ttgccatgga
     8881 ccaatagatt taccaaacca cgcatcgttg gagatagtca gcaggtagtc ggtatccggg
     8941 cggaagttat cgcgcacttg ctcggcgaga ataatctcgt agcaaatagc cgcagtaagc
     9001 tcaataccat ttgccgacag cggcggctgg atatatggcc cacggctgaa cgacgacatc
     9061 ggcagatcaa agaacggtgc taacggacgc agaatcgact ccagcgggac aaactcgcca
     9121 aacggcacca gatggttttt gttatagcga tcggctgatt cgtagctgta cggtgcacct
     9181 ttacccagcg tgatgatggt gttgtaggta tcgtagcggt tctgcttatt gagacgcgcg
     9241 tcgacaatcc cggttaccag cgagctacct ttatcacgca actcaccgtc cagtgctttg
     9301 aggaacggtt gctgattaat ttccagatcg gttatcgccg actccggcca gataatcaac
     9361 gatgatttgc ccatcagcgg tgccgttgcg ttgtagtaaa tctttagcgt attaagaagc
     9421 tggccttcgt cccatttcag cgattgcgga atatcgccct gaaccatcga aacctgaatg
     9481 gttttctccg gttgcggggt aaaccattgg atgtaacgca gcgggaaggg tagggcaaac
     9541 agcacgacgg ccaccaccag cggacgccag ttgcgtttga ccaacgccag tgccagcagg
     9601 ccactaacca tcatcagcag gaagttaatg gcttccacgc ccattagcgg tgccagccct
     9661 tttaacgggc catcaatctg gctatagccg aactgtaacc acgggaagcc agtcagcacc
     9721 caaccgcgca gaaactcggt cacttgccag agagcagggg tggcaatcgc cacgcgcagc
     9781 caggtggttt tcggccacag acgcgacagc acgccagcaa acagtccggt atacagcgac
     9841 aaatacgccg ccagcagcac caccaggaag atgttaaccg ggccaggcat cccgccaaag
     9901 gtcgcgatgc tgacatagac ccagttaata ccgctgccaa agaggccaaa tccccagcaa
     9961 aagccaatag cggcagactg gagtgggcga cggttaaagg tcaacgcctg aagccccatc
    10021 agcgaaataa tcgccgcagg ccagacgtcg taaggagaga aggccagcgt tccgcaggca
    10081 ccgaataata acgccagcag caggcgaatg cgctggcgtt caattaatga ggcaaaatcc
    10141 atgtagttat ctatccagtt tcggttttat tcatccagct ttggctgggg tgagtcatcc
    10201 gggattttga catgaacctg aataatacgc cgactatcgg ccatcgccac tttgaactgg
    10261 taaccgtcga tgtcgatagt ttcgccacgc gccggaagat gcccaaacgc ctgcatcacc
    10321 agaccaccga tagtgtcgac ttcttcatcg ctaaagtggg tgccgaacgc ttcgttgaag
    10381 tcttcaatgg aagccagtgc gcgcacggtc caggtatgac gactcagctg acggaagtcg
    10441 atatcatctt cttcgtcata ctcgtcttca atctcaccaa caatcagttc caggatgtct
    10501 tcaatggtca ccagaccgga aaccccaccg aattcgtcaa taacgatcgc catgtggtaa
    10561 cgctgagagc gaaactcttt cagcatccgg tctacgcgct tactttcagg aacgacaacc
    10621 gcctgacgta acactttgtc catgctgaag gcttcagcat cgctgcgcat aaacggcagc
    10681 aagtctttcg ccatcagaat cccttcaatg tgatctttgt cttcgctaat caccgggaaa
    10741 cgtgagtggg cggactcgat gatgacatca aggcattcgt ccagcgtctg gttgcgtttc
    10801 agggtaatca tctgggagcg ggggatcatg atgtcgcgaa cgcgttggtc tgcgatgtcc
    10861 atcacccctt cgagcatatc gcgcgtatct tcgtcgataa ggtcgttctg cccggaatca
    10921 cggatcagcg ccagcagttc gtcacggttt ttcggttcac cgtggaaaag ttggctgagt
    10981 aacagggaga aaaatccctt cttgttgctt atcgtgtcac tactgtgtga attgtcgtcg
    11041 ctcatggcgt cgtatgggtt ctcatgttag ttaatcaaaa cgccgtcgtt aatcaccaac
    11101 ggcggggacg tctgccagtc aaatgcctgg caaattattc tttctcggca atgtacggat
    11161 cctcatagcc cagagcaagc ataatctctg tttcgagggc ttccatttct tctgcttcgt
    11221 catcttcaat gtgatcgtaa cctaacaaat gcagactgcc gtgcaccacc atatgcgccc
    11281 agtgcgcctc cagtggtttg ccttgctcct gagcttcctt ctcaaccacc tgacggcaga
    11341 taaccagatc gcccagtagc gacatctcca tgccaggcgg cacttcaaac gggaaggaga
    11401 gcacgttggt cggcttatcc ttaccgcgat aggtcagatt cagactgtgg ctttcggcgg
    11461 tatcgaccac gcgaatagtc acttccgact cttcctgaaa ctgcgggatc accgcattca
    11521 gccatgtttg aaactggctc tcttccggta acccggaatt atcttcacat gccagttgta
    11581 aatcgaggat cacctgactc atttttgttc ctgttcttcg cgcttgcgtt ctgctgccag
    11641 cgccgcttta cgtttttgtt ctgcttcttc ccaggcttca taagcgttaa cgatacgcgc
    11701 caccaccggg tgacgaacca cgtcttcgct gtggaagaag ttaaagctga tctcttcgac
    11761 atcggccagc acttcaatgg catgacgtaa gcctgattta gtattacgcg gcaggtcgat
    11821 ctgtgtgacg tcgccggtga taaccgcttt tgagttaaaa ccgatacggg tcaggaacat
    11881 cttcatctgt tcgatggtag tgttctggct ctcatcgaga atgataaacg cgtcgttcag
    11941 cgtacgacca cgcatatagg ccagcggcgc gacttcaata acgttgcgct caatcagttt
    12001 ctcgactttc tcaaagccca gcatttcaaa cagcgcgtcg tacagtgggc gcagatacgg
    12061 gtctactttc tggcttaaat cgccaggcag gaagcccagt ttctcaccgg cttcgaccgc
    12121 cggacgagtc agcagaatac ggcgaatttc ctgacgctcc agggcatcaa ctgccgcagc
    12181 cactgccagg taggttttac ccgtacccgc cgggccaacg ccgaaggtaa tgtcatggtc
    12241 gagaatattg gcgatgtact gcgcctggtt tggcgtgcgc ggcttaatta cgccgcgttt
    12301 agttttgata ttgaccgctt tgccgtactc cggcacgctc tccgcgcttt gctccagtac
    12361 ccgcgcttct ttaatcgcaa ggtggatctg ttccggttcg atatcctgaa tctgaccgcg
    12421 catcggggca gtatcgacat acaggctacg cagaatgtct gccgcagcgg tgacgcaaat
    12481 cggacggccg gtcagtttaa agtggttatc gcggcgattg atctcgatgc cgagacggcg
    12541 ttcgagctgc ttgatgttgt catcaaacgg gccgcacagg ctcaacagac gcgcattgtc
    12601 tgctggctcc agggtgattt cgcgagtgtc tatgttcaaa ccgttcctct tatctgtatg
    12661 ccgccggaag ctgaacattc accggcctat aaggaaatta ttcacgccac aggaaaaagg
    12721 cgcaagcatt gcgataaaga tggggataaa gagagaaaaa acaaggccca ccggaacggc
    12781 aggcctgaga attacggctg ataataaccc acgccaaggt cgttttcttt gcgggtgcgg
    12841 gcaatcaccg actccggtgt ttctgccatg cgcagaccca tttcatcttc agttcgcacc
    12901 actttaccgc gcagagagtt cgggtagacg tcggtaattt ctacatcgac gaatttaccg
    12961 atcatatccg gtgtgccctc gaagttgacc acgcggttat tttcggtacg cccggaaagc
    13021 tccatgatgc tcttacgcga agtcccttct accagaatac gctgggtggt gccgagcatc
    13081 cgacggctcc atgccatcgc ttgctgattg atacgttctt gcagaatata cagacgctgc
    13141 ttcttctctt cttccggaac atcatcaacc atatcggcgg caggcgttcc tggacgtgca
    13201 gagaagataa agctgtagct catgtcgaaa ttgacgtcgg caatcagctt catcgttttc
    13261 tcgaagtctt cggtggtttc gccagggaag ccaacaatga agtcagaact gatctgaata
    13321 tccggacgcg ccgcacgcag tttacggatg atcgctttgt actccagcgc cgtatgggta
    13381 cgtcccatca ggttcagaat gcgatcggaa ccgctctgta ccggcagatg caggaagctc
    13441 accagctcag gcgtgtcgcg gtacacttcg atgatatcgt cggtgaattc gatcggatgg
    13501 ctggtggtaa agcgaatacg atcgatcccg tcgatcgcag caaccagacg caacagatcg
    13561 gcaaacgatc cggtggtgcc gtcgtagttc tcaccacgcc aggcattcac attctgaccg
    13621 agcaggttga cttcacgcac gccctgagcc gcaagctggg caatctcaaa cagaatatcg
    13681 tcagacggac ggcttacctc ttcaccacgg gtgtaaggca ccacgcagta ggtgcaatat
    13741 ttattgcagc cttccatgat ggagacaaac gcggtcggcc cttcggcgcg cggttccggc
    13801 agacggtcaa acttctcgat ttccgggaag ctgatatcta caaccgggct gcggtcgccg
    13861 cgcacggagt tgatcatctc cggcagacgg tgcagcgttt gcggcccaaa aataatatcg
    13921 acatagtggg cgcgctggcg aatgtgctcg ccttcttgcg atgccacgca gccaccgacg
    13981 ccgataatca ggtctggatt cttctctttt aacagtttcc agcgacccaa ctgatggaag
    14041 actttttcct gagccttctc gcggattgag caggtgttca gcagtagcac atccgcttct
    14101 tccgccacgt cggtcagttg atagccgtgg gtggcatcca gcagatcggc catcttcgat
    14161 gaatcgtact cgttcatctg acagccccag gttttaatat ggagtttttt ggtcatcgac
    14221 ttgctcttgc gaaatagtag ccaggaatgc agggcgcata gtgtaatgct ttgctgccgt
    14281 tgtgaccagt atgagcgtta tcagcccttg ggggtaaaaa tcctgtaaac ttaaagcata
    14341 ttgctaacag gatgattgac catgacaaat caaccaacgg aaattgccat tgtcggcgga
    14401 ggaatggtcg gcggcgcact ggcgctgggg ctggcacagc acggattttc ggtaacggtg
    14461 atcgagcacg cagaacctgc accgtttgtc gctgacagcc aaccggacgt gcggatctcg
    14521 gcgatcagcg cggcttcggt atcattgctt aaagggttag gggtctggga tgcagtacag
    14581 gctatgcgtt gccatcctta ccgcagactg gaaacctggg agtgggaaac ggcacatgtg
    14641 gtatttgacg cagccgaatt aaagttgccg ttgcttggct acatggtgga aaacacagtg
    14701 ctgcaacagg cgttgtggca ggcgctggaa gcgcatccga aagtaacgtt acgtgtgcca
    14761 acctcgctga ttgcattaca tcgagataat gatcttcagg agctggaact gaagggcggt
    14821 gaaacgatcc gcgcgaaact ggtgattggt gccgacggcg caaattctca ggtgcggcag
    14881 atggcgggaa ttggcgttca tgcctggcag tatgcgcagt cgtgcatgtt gattagcgta
    14941 cagtgtgaga acgatcccgg cgacagcacc tggcagcaat ttaccccgga cggaccgcgt
    15001 gcgtttctgc cgttgtttga taactgggca tcgctggtgt ggtatgactc tccgacgcgt
    15061 attcgccagt tgcagaatat gaatatggcg caactccagg cggaaatcgc gaagcatttc
    15121 ccgtcgcgtc tgggttacgt tacaccgctt gccgctggtg cgtttccgct gacgcgccga
    15181 catgcgttgc agtatgtgca gccggggctt gcgctggtgg gcgatgccgc gcataccatc
    15241 catccgctgg cggggcaggg ggtgaatctt ggttatcgtg atgtcgatgc cctgattgat
    15301 gttctggtga atgcccgcag ctacggtgaa gcgtgggcca gttatcctgt cctcaagcgt
    15361 taccagatgc ggcgcatggc ggataacttc attatgcaaa gtggtatgga tctgttttat
    15421 gccggattca gtaataatct gccgccgctg cgttttgtgc gtaatctcgg gttaatggcg
    15481 gcagagcgtg ctggcgtgtt gaaacgtcag gcgctgaaat atgcgttagg gttgtagcct
    15541 tacaacattg ccggatgcgt gccagccgta ggtcggataa gacgcggcag cgtcgcatcc
    15601 gacattgaag gataagacgt gtcaacatcg cattcgacat tgaatgaacg cagaaaagca
    15661 aaaagctcgc cgaagcgagc ttttttaatg tggctggggt acgaggattc gaacctcgga
    15721 atgccggaat cagaatccgg tgccttaccg cttggcgata ccccaactgg gtgcacttac
    15781 gatgtaagcg tcttgaataa attggctggg gtacgaggat tcgaacctcg gaatgccgga
    15841 atcagaatcc ggtgccttac cgcttggcga taccccaaca aattggtttt tgaatttgcc
    15901 gaacgtattc gacacattca gaatttggtg gctacgacgg gattcgaacc tgtgacccca
    15961 tcattatgag tgatgtgctc taaccaactg agctacgtag ccagattatt tcttcgatgg
    16021 ctggggtacc tggattcgaa ccagggaatg cc
//
LOCUS       DAZPFP010000078        15526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPFP010000078 DAZPFP010000000
VERSION     DAZPFP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.078"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHUR4_002346"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHUR4_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR1455911.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHUR4_002347"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHUR4_002347"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR1455912.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHUR4_002348"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="ACHUR4_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHUR4_002349"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHUR4_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1455913.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHUR4_002350"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHUR4_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1455914.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHUR4_002351"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHUR4_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1455915.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHUR4_002352"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHUR4_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1455916.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHUR4_002353"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHUR4_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1455917.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHUR4_002354"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHUR4_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1455918.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHUR4_002355"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHUR4_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1455919.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHUR4_002356"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHUR4_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1455920.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHUR4_002357"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHUR4_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1455921.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHUR4_002358"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHUR4_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1455922.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHUR4_002359"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHUR4_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1455923.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHUR4_002360"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHUR4_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1455924.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHUR4_002361"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHUR4_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1455925.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHUR4_002362"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHUR4_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1455926.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHUR4_002363"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHUR4_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1455927.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHUR4_002364"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHUR4_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1455928.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHUR4_002365"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHUR4_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1455929.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHUR4_002366"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHUR4_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1455930.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHUR4_002367"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHUR4_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1455931.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHUR4_002368"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHUR4_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1455932.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHUR4_002369"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHUR4_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1455933.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHUR4_002370"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHUR4_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1455934.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHUR4_002371"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHUR4_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1455935.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHUR4_002372"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHUR4_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1455936.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHUR4_002373"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHUR4_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1455937.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHUR4_002374"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHUR4_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1455938.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHUR4_002375"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHUR4_002375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1455939.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHUR4_002376"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHUR4_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1455940.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3642 c         4130 g         3747 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ttggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAZPFP010000079        15412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPFP010000079 DAZPFP010000000
VERSION     DAZPFP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.079"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHUR4_002377"
     CDS             complement(<1..1177)
                     /locus_tag="ACHUR4_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1455941.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHUR4_002378"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHUR4_002378"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUR4_002379"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUR4_002379"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1455942.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHUR4_002380"
     CDS             2804..4306
                     /locus_tag="ACHUR4_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1455943.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUR4_002381"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUR4_002381"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1455944.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUR4_002382"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUR4_002382"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1455945.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUR4_002383"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUR4_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1455946.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUR4_002384"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUR4_002384"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1455947.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHUR4_002385"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHUR4_002385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1455948.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHUR4_002386"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHUR4_002386"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1455949.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHUR4_002387"
     CDS             complement(11871..13067)
                     /locus_tag="ACHUR4_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1455950.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHUR4_002388"
     CDS             13260..13763
                     /locus_tag="ACHUR4_002388"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1455951.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUR4_002389"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUR4_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1455952.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHUR4_002390"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHUR4_002390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1455953.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZPFP010000080        15360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPFP010000080 DAZPFP010000000
VERSION     DAZPFP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.080"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            185..1474
                     /gene="hpaG"
                     /locus_tag="ACHUR4_002391"
     CDS             185..1474
                     /gene="hpaG"
                     /locus_tag="ACHUR4_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1455954.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1471..2937
                     /gene="hpaE"
                     /locus_tag="ACHUR4_002392"
     CDS             1471..2937
                     /gene="hpaE"
                     /locus_tag="ACHUR4_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1455955.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2939..3790
                     /gene="hpaD"
                     /locus_tag="ACHUR4_002393"
     CDS             2939..3790
                     /gene="hpaD"
                     /locus_tag="ACHUR4_002393"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1455956.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3800..4180
                     /locus_tag="ACHUR4_002394"
     CDS             3800..4180
                     /locus_tag="ACHUR4_002394"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1455957.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4248..5051
                     /gene="hpaH"
                     /locus_tag="ACHUR4_002395"
     CDS             4248..5051
                     /gene="hpaH"
                     /locus_tag="ACHUR4_002395"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1455958.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5062..5865
                     /gene="hpaI"
                     /locus_tag="ACHUR4_002396"
     CDS             5062..5865
                     /gene="hpaI"
                     /locus_tag="ACHUR4_002396"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1455959.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            5887..7242
                     /gene="hpaX"
                     /locus_tag="ACHUR4_002397"
     CDS             5887..7242
                     /gene="hpaX"
                     /locus_tag="ACHUR4_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1455960.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            7252..8142
                     /gene="hpaA"
                     /locus_tag="ACHUR4_002398"
     CDS             7252..8142
                     /gene="hpaA"
                     /locus_tag="ACHUR4_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1455961.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            8169..9106
                     /locus_tag="ACHUR4_002399"
                     /pseudo
     CDS             8169..9106
                     /locus_tag="ACHUR4_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            9333..10891
                     /gene="hpaB"
                     /locus_tag="ACHUR4_002400"
                     /pseudo
     CDS             9333..10891
                     /gene="hpaB"
                     /locus_tag="ACHUR4_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            10909..11421
                     /locus_tag="ACHUR4_002401"
     CDS             10909..11421
                     /locus_tag="ACHUR4_002401"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1455962.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            11814..13964
                     /gene="btsT"
                     /locus_tag="ACHUR4_002402"
     CDS             11814..13964
                     /gene="btsT"
                     /locus_tag="ACHUR4_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1455963.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14014..14217
                     /locus_tag="ACHUR4_002403"
     CDS             14014..14217
                     /locus_tag="ACHUR4_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1455964.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14228..15184
                     /gene="yjiA"
                     /locus_tag="ACHUR4_002404"
     CDS             14228..15184
                     /gene="yjiA"
                     /locus_tag="ACHUR4_002404"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1455965.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15253..>15360)
                     /locus_tag="ACHUR4_002405"
     CDS             complement(15253..>15360)
                     /locus_tag="ACHUR4_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1455966.1"
                     /translation="FYLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         3685 a         4053 c         4058 g         3564 t
ORIGIN      
        1 attttatcaa aattgatgat attttattaa cttactgata tttaatattt tattatcttt
       61 aaatgtaaat agtttgttaa ttagatcaca tttatatcac ttacttttgc caaactacgc
      121 aatcgggaat aaaagtaatc attaacatat taatgattaa gatgatcgaa tccgaggagt
      181 ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcctg
      241 gcaggaagcg ttccagcaat ctccctacaa agccccgcct aaaactgcgg tctggtttat
      301 taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa
      361 agtactgagc ggtgcgactg ttgcgctgat tgtggggaaa acggcgacga aagtacgtga
      421 agaagatgcg gcagagtaca tcgccggata tgcgctggat aacgacgtca gcctgccgga
      481 agagagcttt taccgtccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg
      541 cgaaaccgtg gctctcagca atgtcgataa tctgactatc tataccgaga tcaacgggcg
      601 tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcacagc tgctgagcgc
      661 cctgagcgaa tttgccacgc tgaatccagg cgatgctatt ctgctcggca cgccacaggc
      721 gcgcgtggaa atacagccaa gcgatcgcgt gcgtgttctc gcagaaggtt tcccgccgct
      781 ggaaaatccg gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgctgcc
      841 acacccgcac ggtacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact
      901 ggaatttaag ccaccggaag agccgctggt gttcctgaaa gcgccgaata ccctcactgg
      961 cgacaaccag acctccgtgc gcccgaacaa tattgaatac atgcactacg aagcggagct
     1021 ggtggtagtt attggcaagc aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt
     1081 cgcgggctac accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg
     1141 ccctaacctg cgggtcaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc
     1201 gaaagaggcg atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt
     1261 acgccagcaa ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt
     1321 aagcgaattt atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt
     1381 atctgacgtg gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg gccgcctggt
     1441 gaaccgaatt gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg gatcaacggc
     1501 aaaaatgttg caggtaacga ctacttccag actaccaatc cggcaacggg tgaagtgctg
     1561 gcggatgtgg cctctggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag
     1621 gcgttcccga aatgggctga tctgccgatg aaagagcgtg cgcgcctgat gcgccgtctg
     1681 ggcgatctga tcgaccagaa cgtgcctgag atcgccgcga tggaaaccgc ggacacgggc
     1741 ctgccgatcc atcagaccaa aaatgtgttg atcccacgcg cttctcacaa ctttgaattt
     1801 ttcgcggaag tctgccagca gatgaacggc aagacctatc cggttgacga caagatgctc
     1861 aactacacgc tggtgcaacc ggtaggcgtt tgtgcgctgg cgtcgccgtg gaacgtgccg
     1921 tttatgaccg ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtgctg
     1981 aagatgtcgg aactctcccc gctgaccgcc gaccgcctgg gtgagctggc gctggaagct
     2041 ggtattccgg cgggcgtgct aaacgtggta cagggctacg gcgcaaccgc aggcgatgcg
     2101 ctggtccgtc atcatgacgt gcgtgccgtg tcgttcaccg gcggtaccgc caccgggcgc
     2161 aacatcatga aaaacgccgg gctgaaaaaa tactccatgg agctgggcgg taaatcaccg
     2221 gtgctgattt ttgaagatgc cgatattgag cgtgcgctgg acgccgccct gttcaccatc
     2281 ttctcaatca acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcagagcatc
     2341 tatccagaat tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg
     2401 aacgatccga atacccaggt tagggcgctt atcagtcagc aacactggga aaaagtctcc
     2461 ggctatatcc gtctcggcat tgaagaaggc gcaaccctgt tggcgggcgg cccggataaa
     2521 ccgtccgacc tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac ggtgctggcg
     2581 gacgtagata accgtatgcg cgttgcccag gaagagattt tcgggccggt cgcctgcctg
     2641 ctgccgttta aagacgaagc cgaaggctta cgcctggcaa acgacgtgga gtacggcctc
     2701 gcgtcgtaca tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa
     2761 gcaggcatgg tgttcgtcaa cacccagaac gtgcgcgacc tgcgccagcc atttggcggc
     2821 gtaaaagcct ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa
     2881 atgaagaacg tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat
     2941 gggtaagtta gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact
     3001 accaggaaaa aaccacggtt gtcgccaggg tgcgatcgac gggcataaag agatcagcaa
     3061 gcgttgccgg gaaatgggcg tcgataccat tatcgttttc gatactcact ggctggtcaa
     3121 cagtgcttat cacatcaact gtgcagacca ttttgaaggc gtctacacca gcaacgagct
     3181 gccgcatttt attcgcgaca tgacctacaa ctacgagggc aacccggagt tggggcagct
     3241 aattgccgag gaagccttaa agctcggcgt gcgggcaaaa gcgcacaata ttcccagcct
     3301 gaaactggaa tacggcacgc tggtaccgat gcgctacatg aatgaagaca agcacttcaa
     3361 agtggtctcc atttcggctt tctgcacggt tcacgatttt gccgacagcc gcaagctggg
     3421 cgaagcgatt ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg
     3481 ttcgttatcg caccgcttta ttgacgatca gcgtgcggaa gaagggatga acagctacac
     3541 ccgcgagttc gaccgccaga tggacgagcg tgtggtgaag ctgtggcgcg aaggccagtt
     3601 caaagagttt tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat
     3661 gcacgacacg gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga
     3721 gtttattacc gagctgttcc caagctctgg caccggtcag gttaacgctg ttttcccgct
     3781 gcccgcgtaa ggacgtttta tgccgcactt tatcgtggaa tgtactgaaa atattcgtga
     3841 agaagcccgc ttacctgagc tgtttgccag tgttaacact gcactggcgg caacggggat
     3901 ttttccgctt gggggaatac gtagccgggc gcactggatc gatacctggc aaatggctga
     3961 tggcaagcac gattacgcct ttgtgcatat gacgctaaaa atcggcagcg gacgcagtct
     4021 ggaaagccgt caggaagtgg gcgaaatgct gtttgatctg attaaaacgc acttcgcctc
     4081 actgatggag agccgttacc tagcgctttc atttgagatt gcagagctgc acccgacgtt
     4141 gaatttcaaa cagaacaacg tacacgcgtt gtttaaataa atttgcacca gcttccaggc
     4201 cggatgataa ggcgctgccg catccggcaa tgccacagga tatcgctatg ttcgataaac
     4261 acacccacac cctgatcgcc cagcgtctgg atcaggcaga aaaacagcgc gaacagatcc
     4321 gcgcgatctc gctggattac ccggagatca ccatcgaaga cgcctacgcc gtgcagcgcg
     4381 aatgggttcg cctgaaaatc gccgaaagtc gcacgctgaa aggccacaaa atcggtctga
     4441 cctcgaaagc gatgcaggca agctcgcaga tcagcgaacc ggattacggt gcactgctgg
     4501 acgacatgtt cttccacgat ggcagcgata tcccgaccga tcgctttatc gtgccgcgca
     4561 ttgaagtgga gctggctttt gtgctggcaa aaccgctgcg tgggccaaac tgcacgctgt
     4621 tcgacgttta caacgccacg gactatgtga tcccggcgct ggagctgatc gacgctcgct
     4681 gccacaacat cgatccggaa acccagcgcc cgcgcaaagt attcgacacc atttccgata
     4741 acgccgccaa cgccggagtg atcctcggag gtcgtccgat caaaccagac gaactggatc
     4801 tgcgttggat ctccgccctg atgtatcgca atggcgtgat tgaagaaacc ggcgtcgccg
     4861 ctggtgtgct gaatcatccg gcaaacggcg tggcctggct ggcgaacaaa ctcgcaccgt
     4921 atgacgtaca actggaaacc gggcaaatca ttctcggtgg ttcgttcacc ctcccggttc
     4981 cggcgcgtaa gggcgacacc ttccacgtcg attacggcaa catgggctcc attagctgcc
     5041 gctttgttta aggagagaac gatggaaaac agttttaaag cggcgctgaa agcaggccgt
     5101 ccgcagattg gattatggct ggggctgagc agcagctaca gcgcggagtt actggccgga
     5161 ccaggattcg actggttatt gatcgacggt gagcacgcgc cgaataacgt gcaaaccgtg
     5221 ctcacccaac tacaggcgat tgcgccctac cccagccagc cagtggtacg cccatcgtgg
     5281 aacgatccgg tgcaaatcaa acaactgctg gacgtaggca cacaaacctt actggtgccg
     5341 atggtacaaa acgccgacga agcccgtaaa gcggtacgcg ccacccgtta tccccccgcc
     5401 ggtattcgcg gtgtgggcag tgcgctggct cgcgcctcgc gctggaatcg cattcctgat
     5461 tacctgcaaa aagccaacga tcaaatgtgc gtgctggtgc agatcgaaac gcgtgaggca
     5521 atgaagaact taccgcagat tctggacgtg gaaggggtcg acggcgtgtt tatcggcccg
     5581 gcggatctga gcgccgatat gggttatgcc ggtaatccgc agcacccgga agtacaggcc
     5641 gccattgagc aggcgatcgt gcagatccgc gaagcgggca aagcgccggg gatcctgatc
     5701 gccaatgagc aactggcaaa acgctatctg gaactgggcg cgctgtttgt cgccgtcggc
     5761 gttgacacca ccctgctcgc ccgcgccgcc gaagcgctgg cagcacggtt tggtaatagt
     5821 tcttctattt cgatggcgaa gcaaaacaac aactctgtct actaaacgta cgatgtggag
     5881 cgcatcatga cgacctctac cctgcaatat aaagataata aaactgttga aattgaacat
     5941 cgtgtcatta ataaattatt tcgtcgccta atcatttttc tttttattct gtttgttttt
     6001 tcgtttcttg atcgcatcaa tataggattt gccggactga caatgggcaa ggatcttggc
     6061 ctgacgtcca caatgtttgg cctcgccgca acactctttt atgtcacgta cgtcatgtgc
     6121 gggatcccca gtaatatgat gttagcgaaa attggcgctc gtcgatggat cgctgggatc
     6181 atggtggtct ggggaattgc ttccacctgc acaatgtttg caacaagccc cgaaacactc
     6241 tatctactgc gtatgctggt tggtattgct gaggcgggtt ttttacctgg tattttggtt
     6301 tatctgacct ggtggttccc ggcatatcat cgggccagag ccaatgcctt atttatgata
     6361 gcgatgccag tcactatgat gctgggattg atcctttcag gctacattct ggccttggat
     6421 ggactgtgga atctaaaagg ctggcaatgg ctctttttgc tggaaggtct gccatcggtc
     6481 attcttggtg tcgtcacctg gttttatctc aacgacacac cagaccaggc gacctggctt
     6541 gatgaaaacg aaaaacaggc tttaaaaacg atgattctcc gtgaacaaga gagtgcattt
     6601 gcccatgcat ctgttccacg ctcaacctta cgtgaagtcc tgaccccagc catcatgcta
     6661 tatacacttg cttatttttg cccgactaat actctgagtg cgattaatat ctggacgccg
     6721 caaatcctac agagtttcaa tactggcagt agcaacatca tgattggttt gctggcggcc
     6781 atcccccaat tctgcacaat ttttggcatg atctggtgga gtcgtcgttc cgaccgtctt
     6841 aaagaacgca aaaagcatac gattcttccc tacctgtttg ccgctgccgg atggttactc
     6901 gcttctgcga cagatcacag cctcattcaa ctgagcagca tcattatggc ctcaatggga
     6961 tcatttaccg cgatgactat cttttggaca acaccggatc aagttataag cttgcaatcg
     7021 cgtgccgttg cgatggctgt catcaatgct gtcggcaatg taggttcagc agtcagtcca
     7081 ttacttatcg gtattttgaa agatgcaacc ggcagtttta gctcaggttt gtggtttgta
     7141 gcaggattgc tggtactggg cgcgttagtc atgacccgaa tccctatggg gagtcgggaa
     7201 cactctcttt catctgaact tatcgcacca aaaccgcaat aaggagtcat gatgtccagt
     7261 gaagctatct ttgaacatgg ttatgaaaat attgatttgg tccgggaata tgatgcccgc
     7321 tacgctggtg aagatgtgca ttatgaaaca tttgcccggt tagcgaattt ctttggacgc
     7381 gatatgcgcc ctcactggca cgatagatat tttcaactcc attatctggt cactggacgc
     7441 attactttac aactggatga gcacttctat tctgtgcgcg caccgctttt tgtactaaca
     7501 ccaccatcgg tgccacatac tttctttact gatcctgata gcgatgggca tgttctgacc
     7561 gtacgtcagg agttaatttg gcccctggta gaaaagctct ggcctggtag tggagatgcc
     7621 gcaatgctac aaggtatctg tctgtcactg gagggaatgg aacatacact ggcggcatta
     7681 aatcattatt ggccgataat atccctggag tttcaacaaa ataagcaagg acgggaaatt
     7741 ctattgcaat cactggcgca aagtattttt acaatcctac tgcgtgaagc accaccaaat
     7801 gatatttcaa catgcagcgt gcgcggcgag atgtgtttat tccagaagtt taatcggctg
     7861 attgacgaaa attatcgcca acatttaatg gtccctgaat atgcaacaat gctcggtatg
     7921 agtgaatcac ggcttaccga gctgtgccga cgttttgcca atcaatcgcc gaagagattg
     7981 attttcgaac gagtgagccg ggaagccaga cggcttctgc tctatagcga gcaaagcatc
     8041 aaccagatag cccttgatct tggctacaaa gatcccgcct atttcgcccg ttttttcaac
     8101 cgaatgatgg gttgctcacc aacacaattc cgtggtcgct gaccatttat caacctgaga
     8161 gactcgacat gaaaatatct ttacgctcac tatttgtggc gtgcctgatt gcgccagtgg
     8221 cattcaccta cgcctccgaa ccgcaataaa tgaaaattgt actacttgga acgaaaggtg
     8281 gcccttcttt attaaaagct gaccgtttac cgatggcaac ggcacttatt attggcaata
     8341 aaacctggtt aattgatgct ggttacgggg ccagcttaca actggtaaaa aataatattc
     8401 cactgcgcca gattgataat attttactca cccatttaca cagtgatcat attctggact
     8461 atccatcagt cttaatgaat gcatgggcta gcggtctgca ggacaaaact attcatgtat
     8521 tcggtccgcc aggcacccga gccatgaccg ctgccagttg gcaggtcttc gatcgggaca
     8581 tcacactgcg tatggaagaa gaaggaaaac ccgatcctcg caaactcgtt acagctcaag
     8641 atgttggtga aggcgtagtt tataaagatg ataccgtgac cgtgactgca ttaaaggtcc
     8701 ctcattctcc tttccctgac ggtgaggctt ttgcttatcg cttcgacaca caaggcaagc
     8761 gaattgtctt ctcaggagat acctcgtact ttcccccact ggcaactttc tcagaaggcg
     8821 ctgatatact tgtgcatgag gccgtacatg agccctccgt cgctaagctg gcagacagta
     8881 ttggcaatgg taaaacactg gcaaaagcca ttgcttctca ccatacgact attgaggatg
     8941 taggcaaaat tgccaatgaa gcaaaagtga aaaaactcat ccttagccac ctggtaccaa
     9001 tcaccgttcc ggatgaaaca tggaagcaag aagcacagaa aactattcag gtcctatcgt
     9061 cgttggtcat gaaaatatga cgattaccat tccattcgcc ccataaaaaa ttccgtcccc
     9121 gttagccctt gttagcgggg aatattatga tttgccttac tgtaagttga tcacgatcgc
     9181 agtttactat ttaactccac cttgctgtcg aaaagtacca acgttcgcct gaaacgtctc
     9241 ttcccgacaa ccgcgttcaa cgcttcaatt aaataacaaa aacaacacac aaaattaaca
     9301 atcgtattcc gattaatact gtagaggtcg acatgaaacc agaagatttc cgcgccagta
     9361 cccaacgtcc tttcaccggg gaagagtatc tgaaaagcct gcaggatggt cgcgagatct
     9421 atatctatgg cgagcgagtg aaagacgtca ccactcatcc ggcatttcgt aatgcggcag
     9481 cgtctgttgc ccaactgtac gacgcgctgc acaaaccgga gatgcaggac tctctgtgtt
     9541 ggaacaccga caccggcagc ggcggctata cccataaatt cttccgcgtg gcgaaaagtg
     9601 ccgacgacct gcgccagcaa cgcgacgcca tcgctgaatg gtcacgcctg agctatggct
     9661 ggatgggccg taccccagac tacaaagccg ctttcggttg cgcactgggc gcgaatccgg
     9721 gcttttacgg tcagttcgag cagaacgccc gtaactggta tacccgtatt caggaaactg
     9781 gcctctactt taaccacgcg attgttaacc caccgatcgt catttgccga ccgataaagt
     9841 gaaagacgtt tacatcaagc tggaaaaaga gactgacgcc gggattatcg tcagcggtgc
     9901 gaaagtggtt gccaccaact cggcgctgac tcactacaac atgattggct tcggctcggc
     9961 acaagtgatg ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa tggatgccga
    10021 tggcgtgaaa ttaatctccc gcgcctctta tgagatggtc gcgggtgcta ccggctcgcc
    10081 atacgactac ccgctctcca gccgcttcga tgagaacgat gcgattctgg tgatggataa
    10141 cgtactgatc ccatgggaaa acgtgctgat ctaccgcgat tttgatcgct gccgtcgctg
    10201 gacgatggaa ggcggtttcg cccgtatgta tccgctgcaa gcctgtgtgc gcctggcagt
    10261 gaaactcgac ttcattacgg cactgctgaa aaaatcactc gaatgtaccg gcaccctgga
    10321 gttccgtggt gtgcaggccg atctcggtga agtggtggcg tggcgcaaca ccttctgggc
    10381 attgagtgac tcgatgtgtt cagaagcgac gccgtgggtc aacggggctt atttaccgga
    10441 tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga agatcaaaaa
    10501 cattatagaa cgcaacgtta ccagtggcct gatctacctc ccttccagtg cccgtgacct
    10561 gaacaatccg cagatcgacc agtatctggc gaagtatgtg cgcggttcga acggtatgga
    10621 tcacgtccag cgcatcaaga tcctcaaact gatgtgggac gccattggca gcgaatttgg
    10681 tggtcgtcac gagctgtatg aaatcaacta ctccggtagc caggatgaga ttcgcctgca
    10741 atgtctgcgc caggcacaaa gctccggcaa tatggacaag atgatggcga tggttgatcg
    10801 ctgcctgtcg gaatacgacc agaacggctg gactgtgccg cacctgcaca acaacgacga
    10861 tatcaacatg ctggataagc tgctgaaata acgcagcagg aggttaagat gcaattagat
    10921 gaacaacgcc tgcgctttca tgacgcgatg gccagcctgt cggcagcggt aaatattatc
    10981 accaccgagg gcgacgccgg acaatgcggg attacggcaa cggctgtctg ctcggtcacg
    11041 gatacaccac cgtcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa cccggttttt
    11101 cagggcaacg gcaagttgtg cgtcaacgtc ctcaaccatg agcaggaact gatggcgcgc
    11161 cacttcgcgg gcatgacagg catggcgatg gaagagcgtt ttagcctctc atgctggcaa
    11221 aaaggtccgc tggcgcagcc ggtgctaaaa ggttcgctgg ccagtcttga aggagagatc
    11281 cgcgatgtgc aggcgattgg cacacatctg gtgtatctgg tggagattaa aaacatcatc
    11341 ctcagtgcag aaggtcacgg acttatttac tttaaacgcc gtttccatcc ggtgatgctg
    11401 gaaatggaag ctgcgattta agctccagcc ccttcacgca ttcgtcgaag gggcattttt
    11461 attgtgatct caatcacata tctacccgat gaataattct tgcccaaccg ctcaccgcca
    11521 ttcctgaccg cttacatccc taaaataacc actcagttat ttaccttact ttacgcgcac
    11581 gtatctctgg caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa
    11641 acctagaacg gcttcggcct atgattaaca aattactcta tggattttgg tttgcaggaa
    11701 ggcagcaagt gagtgaatcc ccgggagctt acaaaagtaa gtgtcagagg tgaacgaacg
    11761 cagctgccgc acctgtaagc caaaagacga cgagtaaaat gccaggtttt actatggata
    11821 cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt gcattctgcc
    11881 tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc gtggtcgcct
    11941 ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtacatcgcc cagaaggtga
    12001 tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt ctgaactacg
    12061 ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc ggtgctggtc
    12121 cgctggtggg accggttctc gccgcgcaga tgggctacct gcctggcacg ctgtggctgc
    12181 tggcgggggt agtgctggcc ggtgcggtac aggactttat ggtgctgttt atctcctctc
    12241 gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatggggcca gtaccgggga
    12301 ctatcgcgct gtttggctgt ttcttaatca tgatcatcat cctcgccgtt ctggcgctga
    12361 ttgtggttaa agccctggcc gaaagtccgt ggggtgtctt caccgtttgc tcaaccgtac
    12421 cgattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt gtgggtgaag
    12481 tctctgttat tggtatcgtg ctgctggttg cctctatcta cttcggtggc gtgattgctc
    12541 acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc ttcgcgctga
    12601 ttggctatgc gtttgtttcc gcactgctgc cggtgtggct gatcctcgca ccgcgcgact
    12661 atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgctgggt atcgtggtgc
    12721 tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact ggcccgctgt
    12781 ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct
    12841 tccacgcgct gatctcttcc ggtacgacgc cgaaactgct ggctaacgaa accgacgcgc
    12901 gtttcattgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg gcgctggttg
    12961 cagcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct gctggccttg
    13021 gcatcaccat gcctaacctg catgaaatgg gtggcgagaa cgcgccgatc atcatggcgc
    13081 agctgaaaga cgttaccgca cacgcggcag cgaccgtcag ctcctggggc ttcgtgattt
    13141 cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc ctgaaccgtg
    13201 caggtggtgc gccgacgctg gcggtaggta tcgctcacgt gttccacaaa gtgctgccga
    13261 tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg ttcatcctga
    13321 ccgcgctgga tgcgggtacc cgttctggcc gctttatgct gcaagacctg ctgggtaact
    13381 tcatcccgtt cctgaaaaaa accgactctc tggttgccgg tatcatcggt actgcgggct
    13441 gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta
    13501 agagcctgtg gccgctgttc ggtatctcca accagatgct ggcagccgta gcgctggtac
    13561 tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta actgttgttc
    13621 cggctgtatg gctgcttatc tgcaccacct gggcgctggg tctgaaactg ttcagcacca
    13681 acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag attgctaacg
    13741 gtactgacct gacggcgcag cagattgcca acatgaacca catcgttgtg aacaactaca
    13801 ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc ttctacggtt
    13861 tcaaaacctg gcttgcggtg cgtaacagcg acaaacgtac tgacaaagaa acaccgtacg
    13921 ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccgtgt ttagccccgc
    13981 ttcggcgggg ctttgttctt atcagagtga actatgtttg gtaacttagg acaggcaaaa
    14041 aaatatctcg gccaggcggc gaagatgttg attggtattc cagactacga caactacgtc
    14101 gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga attcttccgc
    14161 gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg
    14221 agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg cgggcaaaac
    14281 taccctgctg cgccatattc ttaacgaaca acacggctac aagattgccg tgattgaaaa
    14341 cgaattcggc gaagtctccg ttgatgacca actgattggc gaccgcgcca cgcagatcaa
    14401 aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggagg acgcactact
    14461 cgacctgctg gataatctcg acaagggcaa tattcagttc gatcgtctgg tgattgaatg
    14521 caccggcatg gccgatcccg gcccgattat tcagaccttt ttctcccatg agattttatg
    14581 ccagctctac ctgctggacg gcgtgattgc gctggtggat gcggtacatg ccgatgagca
    14641 gatgaaccag ttcaccatcg cccagtccca ggtgggctac gccgaccgta ttctgctgac
    14701 taaaaccgac gtcgcaggcg aagcagagaa actgcgtgaa cgcctggcgc gcatcaacgc
    14761 ccgcgcaccg gtctacaccg tcacccacgg cgacatcgac ctgggcctgc tgttcaacac
    14821 caacggtttt atgctggaag agaacgtcgt cagcaccaaa ccgcgtttcc actttatcgc
    14881 ggataaacaa aacgatattt cgtcaattgt ggtggaactg gattacccgg tagatatcag
    14941 cgaagtttcc cgcgtgatgg aaaacctgct gctggaatcg gcggataaac tgctgcgtta
    15001 caaagggatg ctgtggattg acggcgaacc taaccgcctg ctgttccagg gcgtccagcg
    15061 cctctacagc gccgactggg acaggccgtg gggcgatgaa aaaccgcata gcacaatggt
    15121 gtttatcggg attcagttgc cggaggaaga gattcgggcg gcgtttgcgg gattgaggaa
    15181 gtaagttcgg gaaagatcaa ctttttgaga gttccaccgc aacattatta cgagtggaac
    15241 tctttagata tgtcagacca accaaagctt cttcaattgc gttttcattt tttcatgtct
    15301 cattttcagt tcagccaatc cccattctga agaattacaa acatcttttg ttaaataaaa
//
LOCUS       DAZPFP010000081        15265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPFP010000081 DAZPFP010000000
VERSION     DAZPFP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.081"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(13..819)
                     /gene="trpA"
                     /locus_tag="ACHUR4_002406"
     CDS             complement(13..819)
                     /gene="trpA"
                     /locus_tag="ACHUR4_002406"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1455967.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(819..2012)
                     /gene="trpB"
                     /locus_tag="ACHUR4_002407"
     CDS             complement(819..2012)
                     /gene="trpB"
                     /locus_tag="ACHUR4_002407"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1455968.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2024..3385)
                     /gene="trpCF"
                     /locus_tag="ACHUR4_002408"
     CDS             complement(2024..3385)
                     /gene="trpCF"
                     /locus_tag="ACHUR4_002408"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1455969.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3386..4981)
                     /gene="trpD"
                     /locus_tag="ACHUR4_002409"
     CDS             complement(3386..4981)
                     /gene="trpD"
                     /locus_tag="ACHUR4_002409"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1455970.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(4981..6543)
                     /gene="trpE"
                     /locus_tag="ACHUR4_002410"
     CDS             complement(4981..6543)
                     /gene="trpE"
                     /locus_tag="ACHUR4_002410"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1455971.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6565..6658)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6635..6679)
                     /gene="trpL"
                     /locus_tag="ACHUR4_002411"
     CDS             complement(6635..6679)
                     /gene="trpL"
                     /locus_tag="ACHUR4_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1455972.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6817..7698
                     /gene="rnm"
                     /locus_tag="ACHUR4_002412"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6817..7698
                     /gene="rnm"
                     /locus_tag="ACHUR4_002412"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1455973.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7695..8315
                     /locus_tag="ACHUR4_002413"
     CDS             7695..8315
                     /locus_tag="ACHUR4_002413"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1455974.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8416..9288
                     /gene="rluB"
                     /locus_tag="ACHUR4_002414"
     CDS             8416..9288
                     /gene="rluB"
                     /locus_tag="ACHUR4_002414"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1455975.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(9328..9918)
                     /gene="cobO"
                     /locus_tag="ACHUR4_002415"
     CDS             complement(9328..9918)
                     /gene="cobO"
                     /locus_tag="ACHUR4_002415"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1455976.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(9915..10673)
                     /locus_tag="ACHUR4_002416"
     CDS             complement(9915..10673)
                     /locus_tag="ACHUR4_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1455977.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10893..11942
                     /gene="sohB"
                     /locus_tag="ACHUR4_002417"
     CDS             10893..11942
                     /gene="sohB"
                     /locus_tag="ACHUR4_002417"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1455978.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(11978..12229)
                     /locus_tag="ACHUR4_002418"
     CDS             complement(11978..12229)
                     /locus_tag="ACHUR4_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1455979.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12609..15206
                     /gene="topA"
                     /locus_tag="ACHUR4_002419"
     CDS             12609..15206
                     /gene="topA"
                     /locus_tag="ACHUR4_002419"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1455980.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3689 a         3907 c         4099 g         3570 t
ORIGIN      
        1 gcggctgtgg gattaactgc gcgtcgccgc tttcatcggt tgtacaaaaa ctttcagtgc
       61 cgccagcatt ttctctggct cattaatatg ttgctcgatg attttaacaa tggccgaacc
      121 agaaatcgcg cccgcagctc ctgcatcaat cgctgctttt acctgatccg gggcggaaat
      181 accaaacccc tgcaatggag gtgcagcgtt gtactctttc agcttcgcaa ccagatgatt
      241 gaggggtaac gcggcgcggt tttctgcgcc ggtcacgccc gctcgtgaca gcaaatatgt
      301 gtaaccacga ccgtaagagg ctatctggcg cagcaggtcg tcatcggcat ttggcgggca
      361 gatgaagata ggtgcgacat tatggcgcaa cgcggcctgg cggaagggcg cggactcttc
      421 aactggcaca tcggcaacca gcaccgaatc gacgccgact ttctcgcact gggcataaaa
      481 ctcatcaatg cctttgttaa acaccagatt ggcatacatc aacaggccaa tgggaatggt
      541 cgggtgtttc tggcgaatca gtgccagcat ttcaaaacat tgtgccggag tcacacctgc
      601 cgcaaaggcg cgcagagtgg cgttttgaat ggtcgggcca tccgccagtg ggtcggagaa
      661 ggggatacct aactccagcg cgtcagcacc ggcttcaatt agcgtatcga taattttcaa
      721 tgactgctca atgcccggat caccgagcgt gacgaaagga acgaatgcgc cttctttgcg
      781 ctccttcaac tgggcaaaca gagattcgta gcgttccatc agatttcccc tcgtgctttc
      841 aaaatatcgt gaacggtgaa gatgtcttta tcgccgcgac cggaaaggtt aaccaccagt
      901 agctgctctt tatccgggtt ttcgcgcatc attttcagcg catgggccag ggcgtgggag
      961 gattccagcg ccgggatgat cccttcgtgc aggcacagcg ttttgaaggc ttcaagggct
     1021 tcatcatcgg taatagacac gtaatcagcg cgtccagtgc tgttaagata cgcgtgttgt
     1081 ggaccgacag acgggaaatc cagtccggcg gagatggagt aagattcttc aatctgcccg
     1141 tcttcggttt gcatcatcgg cgctttcata ccgaaataaa tgcccacgcg accatgtttt
     1201 agcggtgcgc cgtgctcgcc agtttcgata ccgtgaccac caggctccac accaatcagg
     1261 ccgacgttgg tttcattgat gaaatcagca aacatgccga tggcattcga accgccgcca
     1321 acacaggcga taacggcatc cggcaggcga ccttctcttt ccagaatctg cgctttggtt
     1381 tcttcgccaa tcatccgctg aaactcacgg acaatggtcg gataaggatg cgggccagcc
     1441 gcggtgccca gcatatagtg cgcggtttca taactgccgg accagtcgcg tagcgcctca
     1501 ttacaggcat ctttcagcgt ggcggaaccg ctatgtaccg ggatcacttc cgcacccatt
     1561 aagcgcatac gaaaaacgtt aggcgactgg cgttcaacgt ctttggcacc catataaata
     1621 cggcatttca ggccgagcag ggcgctggca agggccgacg ccacgccatg ctgaccggcc
     1681 ccggtttcgg cgatgatttc ggttttaccc atccgcttcg ccagcaacgc ctgccccagc
     1741 acctggttgg ttttatgtgc gccgccgtgc agcaaatctt cacgcttgag atacagcgtg
     1801 gtgttcgtcc cggctgtaat gttctggcat ttggtcagtg cggttggacg cccggcatag
     1861 tttttcagca ggtcgttgaa ctgggcttga aattcaggat ctttttgcgc gctgacaaaa
     1921 gcttcttcca gctggcgcag agcaggcatc aggatttgtg gcacgtacat gccgccaaac
     1981 tcaccaaaat aggggttaag taatgttgtc attgttcctt tccttaatat gcgcgcagcg
     2041 tctggaaaac cgaggccaaa agacgtgcgt ctttgatgcc cggttgcgac tctacagcag
     2101 aattaaaatc aagtccggcg cagccggttt gtgccgcttc cacgcagtta tctgcgccta
     2161 agccccccgc cagcagaacg ttgccaagcg attgaccatt taatagtgac cagtcaaaac
     2221 gttgcccgct tccaccctgg ccgttgtcta aaacatattt atcaacgtgc tgaaactcgc
     2281 gggcgggcag ggtttcaccg acgcttaatg ctttccagat ggcaacatgt gctggcagag
     2341 cttcacgcag cgtatcgata tacagctgat cttcattgcc atgcagttgc actgccgcca
     2401 gcgataacac cttagctttg tccaccacat cggcaatatc gtgattgcgg aacacgccaa
     2461 catactgcaa cggtgccgca gccatcactt cctgcgcctg ttcaacgttg acgcaacgcg
     2521 gtgatgtcgc aacaaaaatc aacccaccgt aaatcgcgcc cgcgtcataa gctgctttag
     2581 catcttgccc acgcgtcagg ccacatactt tattctcacc cagcaacacc cggcgcacgg
     2641 cggcgtgcaa atcgtcatgg gccatcaacg ccgaaccaat cagaaaaccg ttagcgaagt
     2701 ggcttaactc gcgcacctga gcgtaagtat tgatgccgga ttcgctgatt accgttacgt
     2761 tgtgccccag tttcggcgca agctcgcggg tacggttgag atcaatcgac aaatcacgca
     2821 gatcgcggtt gttgatgcca acgacctttg cccctaatgc aatggcgcgc tccagttcct
     2881 cttcattact gacttcggtc agcaccccca tctccagact gtgagcgacg gctgcaagct
     2941 ggcgatattg gtcgtcatcc agtactgaaa gcattaataa gcaggcatcg gcctggtaat
     3001 agcgcgccag atagatctgg taagggtcga taatgaagtc tttacataaa atcggctgcg
     3061 gggcgatttg gctgacgatg aggaggaaat caaagctccc ctgaaaatat ttctcatcag
     3121 tcagcaccga aattgccgaa gcgtaatgtt tataaatggc ggcaatgcgt gccggatcga
     3181 aatcatcacg gatcacgcct tttgacggcg acgctttttt acactccaga ataaacgccg
     3241 tgcgtgcacc ctgtagcgca tcataaaaat gtcgtgtgct cggctgaacc tcattctgaa
     3301 aactggccag cggttgctgc tgtttgcggg tttctaccca aatcgccttg tctgcgacga
     3361 ttttcgctaa aacggtttgc atcatttacc ctcgtgccgc cagcgcggta actctgtcgt
     3421 aagcggaacc actgcgcagt acctcaagaa cggtttgcgc attggcttgc agatcttcat
     3481 ggccatgcag gcgcattaac atggcgacgt tcgcagcgac ggctgcttca tgggcggcgt
     3541 cgcctttacc ttgtaacaag cgtgttaaaa tgtcacggtt ttcttcaggt gttccgcctg
     3601 ccagttgctc ctggtggtag ggagtcaggc caaaatcttc agcggtcaat tgatagctct
     3661 taattttgcc gtcatgcagc tcggcaacga ttgtcggcgc gtgtaatgaa acttcatcca
     3721 tcccgccgct gtgcaccacc gccgcgcgtt gataccccag cacgcgcaag gtttcggcaa
     3781 tcggcagcac cagttccgga ctataaacac caattaacgc cagcggcgga tgcgccgggt
     3841 taatcaatgg ccccagcaca ttgaacaggg tgcgggtttt cagttgctgg cgaaccggca
     3901 tcgcgtggcg gaatccggtg tgatatttcg gcgcaaagag gaaacatacg cctaactcgt
     3961 ccagcgcctg gcgcgattta ttggcgttca tatcaagatt aataccgaat gccgccagca
     4021 gatccgacga gccggattta ctggagacgc tgcggttacc gtgtttcgcc actttcagcc
     4081 cacaggccgc ggcgacaaac gcactggcgg tagaaatatt gatactgttg ctgccgtcac
     4141 cgccagtacc gacgatatca gcaaacagat aatccgggcg cgggaacggc gctgcgtttt
     4201 ccagtagcgc ggttgctgcc ccggcgatct cgttcgggtg ctcaccgcga attttcatgc
     4261 tcaccagcgc cgccgccagt tgttccggct tcagctcgcc acgcaccacc gctgaaaaca
     4321 gctggtggct ttcttgttgg ctaagcgtct gcgcctgata cagtttttcc agaatcggtt
     4381 gcagcgtgtt ggctggctct agtttctgct gcgcccaggc cagcgtttgt tccagcaggc
     4441 gagcgccctg ggtggtgaga atggattccg gatggaactg gaatccacaa acgcgatccg
     4501 catcgtgacg tactgccatc accatgccat taaaatgggc gttgatggtt aaaccggccg
     4561 gaatgttact gccaaccagc gagtgataac gcgccaccgg cagcgggttt gttaatccgg
     4621 caaacatcgc ctgaccgtca tgttcaatgc tcgacgcttt accgtgaaga atttcgcccg
     4681 cctgaccgac atagcccccg taagcttcga caattgcctg atgtccgagg caaatgccaa
     4741 taatgggcag cttgccacgc aagcgggtga ggagttccgg catacaaccg gcttcgctcg
     4801 gcacaccggg gccaggagaa agcatcagta ccggattgct catcgtcgcc aggcgttcaa
     4861 ttaaggtctg cgccggaata tggttgcggt aaatcaccac gttatgacca ttgctgcgca
     4921 actgatctgc caggttgtac gtaaaagagt cgatattatc gagcagcaga atgtcagcca
     4981 tcagaaagtc tcctgtgcat gatgcgcggt ggcaatagcg cgcagtacag cgcgggcttt
     5041 gttacgggtt tcgtcggctt ccgactgcgg aacagaatca aggactacac cagcacccgc
     5101 ttgcacggtg gcgataccgt tttccaccag cgccgagcgg atcacaatgc aggtgtcgag
     5161 atcgccatgc gcggtgaaat aacctaccgc gccgccgtag ctgccgcggc gacgaccttc
     5221 cgcctcggca attaactgca tagcgcgtac tttcggcgca ccgcttaacg tccccatatt
     5281 catacaggcg cgataagcgt gcagggcgtc aagatcgtga cgcagttcgc cgactacgcg
     5341 agagacgagg tgcatcacat aggaataacg gtcaactttg gtgagatcgg cgatgtagcg
     5401 gctgccaggg gtgcaaatgc gtgccagatc attacgggcg agatcaacca gcatcagatg
     5461 ttcagacagc tctttatggt cagtacgcat ttccagttca atacggctgt cgagatctct
     5521 gtccagtgaa ccatcggcgc gacgaccgcg tgggcgtgtt ccggcaatcg ggtagatctc
     5581 aatctggcgg ctggtggcat catacttgag cgagctttcc ggcgacgcgc caaatagggt
     5641 gaaatcatta tcctgcataa aaaacatgta cgggctggga ttactctttt tcagcacgta
     5701 ataggccgcc agcggtgacg ggcagggcag agagaaacgg cgagatggca ccacctggaa
     5761 aatttctcca gcgcgaatcg ctttttgcaa caaacgcact acgccaccga actcttcatc
     5821 gctctgatta cattcacaac gcatatgcgg cacggaaacc actggcagcg gcggcgcggc
     5881 ttcggtcagt tgctgacgta gttcgttcag gcgagcagtg agacgttgtt tttcttcttc
     5941 attcggagca aacaggctgg cctgaatacg ggtgcttttt ttctgatggt caatcaccat
     6001 cagcgtttca gcgagataaa aacagaaatc agggcagtta ttttccgctg acagttgcgg
     6061 taaatcttca aatcccgcca caaggtcata agagaacagg ccgccgaaga acattgcttc
     6121 tcgttcttcc ttcggtacat tcaacagatt ctgcaataaa cggaaagcgt caaaaaccga
     6181 aagggagcat aagcgggcgt cttcatccag cagtggactg acagggggga agcgcagcac
     6241 acggcagttt ggtgattgtt cactttccac acccgcaggc agggcgttat ccagtagtgc
     6301 cagcagggct tcgccattgc cggaaagtgc ctgaattgtg acggtgtcac ctaatgctgt
     6361 aatgcgcagc gcactgtcta tcagcagcag gctttttaaa tcatctttgc tgtcgatatc
     6421 tgcggattcc agcagcagcg ttgccggacg attcccacac aactggtgaa aaagcgcagt
     6481 cgggttgtcg cgataagcgc cttcgcaggt tagcagttcg agagtcggtt tttgtgtttg
     6541 cattgttatt ctctaatttt gttcaaaaaa aagcccgctc attaggcggg ctgggtatct
     6601 gattgcttta cgcatggtga atacactgcc cgtttcagga agtgcgccac caacctttca
     6661 gtacgaaaat tgctttcatt gtcgataccc tttttacgtg aacttgcgta ctagttaact
     6721 ggttcgatga ttaattgtca acagctcatt tcagaatatt tgccagaccc gttatgatgt
     6781 cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg
     6841 atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg
     6901 gtgcaccgtg cagtcgagat gcgcgtcggc accctggcga tcaccgacca tgacaccaca
     6961 gctgcaatcg cgccagcaag agaggaaatt tcacgttcgg gactggcgct gaatcttatt
     7021 cccggcgtgg aaatttccac ggtctgggaa aatcatgaaa ttcatattgt cgggctgaat
     7081 attgatatta ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga acggcgcaat
     7141 cagcgggcgc agcaaattgc cgaaagactt gaaaaagcgc aaatacctgg ggcgctggaa
     7201 ggcgcacaac gactggcgca gggcggcgca gtgacgcgcg gtcattttgc gcgtttcctt
     7261 gtggagtgcg gcaaagccag ttcaatggca gatgtcttta aaaagtatct ggcgcgcggg
     7321 aaaaccggat acgttccgcc acagtggtgt acaatagaac aagctattga tgtcattcat
     7381 cattctggcg gtaaggcggt gctagctcat cctgggcggt acaatcttag tgctaaatgg
     7441 ctgaaaagat tggttgcgca ttttgccgaa caccacggcg acgcgatgga agtcgcgcag
     7501 tgccagcaat cgcccaatga acgcacccag ctggccacac ttgcgcgtca gcatcattta
     7561 tgggcatcac aaggatctga ttttcatcag ccatgcccgt ggatcgaact gggtcgtaaa
     7621 ctctggctgc ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc gcagaacacc
     7681 acagagaggg aattatgagc cagttttttt atattcatcc tgataacccg cagcaacgtc
     7741 tgatcaacca ggcggtggag atcgtgcgta aaggcggggt gattgtctat ccaactgatt
     7801 ccggctatgc gctcggctgt aaaattgaag ataaaaacgc catggagcgt atttgtcgta
     7861 ttcgccagct gccggacggt cacaacttta ccctgatgtg tcgcgatctt tctgaactct
     7921 cgacctattc attcgtggat aacgttgcgt ttcgtttgat gaaaaacaac acgccgggca
     7981 actatacctt catcctgaag gggacgaagg aagtgccacg ccgtctgttg caggagaaac
     8041 gcaaaaccat cggtatgcgt gtgccttcta acccgattgc gcaggcgttg cttgaagcac
     8101 tgggcgaacc gatgctttcc acttcgctaa tgctgccagg cagcgaattt accgaatccg
     8161 atcccgaaga aattaaagac cgtctggaaa aacaggtaga tttgattatt cacggcggct
     8221 atctcgggca gaaaccaaca acggttattg atcttaccga tgatacgccg gtcgtggtgc
     8281 gtgaaggcgt aggtgatgtg aagcctttct tataagtgcg taactctgta tactacgcgg
     8341 ccttaaaatg gcgctatacg gcgccattga ttctcctgtt cgacgcctgt gaaggcgaca
     8401 ccaaaaggaa gctctatgag cgaaaaatta cagaaagtgc tggcccgcgc tggccacggc
     8461 tcccgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga tggcaaaatt
     8521 gctaaactcg gcgatcgcgt tgaagttacc cctgggctga aaattcgtat cgatggtcat
     8581 ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta ttacaaaccg
     8641 gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc cgacggtgtt tgaccgtctg
     8701 ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa cacctgtggt
     8761 ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcatcc aagccgtgaa
     8821 gttgaacgtg aatacgccgt gcgtgtattt ggtcaggttg acgacgcgaa actgcgtgat
     8881 ttgagtcgtg gcgtgcaact ggaagatggt ccggcagctt ttaaaaccat caagttcagc
     8941 ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg taaccgtgaa
     9001 gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg tgttcgttac
     9061 ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct ggacctcgcc
     9121 cagactaact atctgcgcga actggtggag cttccgccag aaaccagctc taaagtcgct
     9181 gtagaaaaag accgtcgtcg catgaaggct aatcagattc gtcgtgcggt gaaacgtcac
     9241 agtcaggtga gcggcagtcg tcgttctggc gggcgtaata acggttaatc aaagtatgcc
     9301 ggacgtcata tccggcattt ttacagatta ataatcaatc cctatttgcg ccttaacacc
     9361 ggcatcaaac gcatgtctga tggggcgcag ctcacttacc gtatccgcca gttcaagaat
     9421 atcccgatga cagcctcgac ccgtgataat caccgtctgt tgatgtggac gttcatttaa
     9481 cgcctgcacc acttcttcca gtggcaaata gtcatacgcc accatatacg tcagttcatc
     9541 aagcaaaacc atatccagtg aggaatcagc aagcatccgc tttgcatgtt gccagacttc
     9601 gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc ccgttgccat
     9661 cacctgaaac tcaacgccat gtggctccag cagattgcgt tcgccattag gccaggtgcc
     9721 tttaataaac tgcacgacgc ccactttttt tccgtgacca actgcgcgtg ttgccgtacc
     9781 aaatgccgcg gtggttttgc cttttccatt gccggtaaag acgatgataa taccgcgttc
     9841 atcctgggcc tgggccacac gagcatctac tttttctttc actcgctgct gacgctgttg
     9901 gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc gtcaaaggtc
     9961 atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg catgatatca
    10021 gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgcttgcgcg cattgcggtg
    10081 cgcgtaccgc ctgggttaat gcagttgacg cgcaggcgct gctgatattc atcggccagt
    10141 acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcacccca gttggctcgt
    10201 ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga tttgagtaat
    10261 aaaggaagca gtgcctgggt gagcataaag gtggcattaa cgttgatctg catgacgtcc
    10321 tgccagacct gcggattttg ttcgctcatt gggcaaacat cgccgagcaa tccggcatta
    10381 tgcaaaacac catccagacg cggataatta acgacaatgc gctgtgccag ttgttggcaa
    10441 ttttcggacg tgcaggtcag caaatcgaga ataaaccact gtggctgacg cccagtttct
    10501 tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa cagaatcact
    10561 gtcgcaccat agcgtgcata cgtcatcgcg gcttcacgac caatcccatc gctggctccc
    10621 gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg catggctact
    10681 cctcaacgac gttgtctgtc gcgctgccag tgtactttat gactttgggg ctttatgcct
    10741 gaaacagaag ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg atactttgtc
    10801 atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg taaactgtga
    10861 gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa tatggtttgt
    10921 ttttggctaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc attattgtca
    10981 atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat ctcagcgaac
    11041 agtataagga gatgaaagaa gaactggccg cggcgctgat ggactcacat cagcaaaaac
    11101 agtggcacaa agcgcagaag aaaaagcaca agcaagaagc gaaagcagca aaagcgaaag
    11161 ccaaactggg cgaggtggca actgacagta aacctcgcgt ctgggtgctg gattttaaag
    11221 gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg gtactcgcag
    11281 cattcaaacc gcaggatcag gttgtgctcc gtctggaaag ccctggtggc atggtgcatg
    11341 gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt cctttaactg
    11401 ttacggtaga caaagtcgct gccagcggcg gttacatgat ggcctgtgtg gcggacaaaa
    11461 ttgtttccgc accgtttgct attgtgggtt ccattggggt ggtggcgcaa atgcccaact
    11521 ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacaccgcc gggcagtata
    11581 agcgcacgct gaccttgctg ggtgaaaata ccgaagaagg gcgggagaaa ttccgcgaag
    11641 agctgaacga aacgcatcag ttatttaaag attttgtgaa gcgtatgcgt ccgtctctgg
    11701 atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta gagaaaggcc
    11761 tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa ggccgtgaag
    11821 tggttaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc ggcagcgcgg
    11881 cagagagcgc cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag ccattgatgt
    11941 aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta atcaaccaga tgatattttt
    12001 ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt ttggctgttg
    12061 gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca ttacgctgcg
    12121 tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg attttgttgg
    12181 cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt atttactcct
    12241 taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc acgctggtgg
    12301 caagagcgcc ttactggcaa ctttggattt tgcatgctaa taaagttgcg tatcggattt
    12361 tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga cattcgacca
    12421 aaaatccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc acctgaattt
    12481 tcgtgaacag agtcacgaca aggggttgat atccgcagag agcgagtcca tatcggtaac
    12541 tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc aaattaggta
    12601 aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc aaaacgatca
    12661 acaagtatct gggttgtgac tacgtggtga aatccagcgt cggtcacatc cgcgatttgc
    12721 cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag acggctaaaa
    12781 agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac ccgtggcaca
    12841 attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct gaactgaaac
    12901 aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc gaaggggaag
    12961 ccattgcatg gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat agccgagtgg
    13021 tgtttaacga aattactaaa aacgcgatcc gccaggcatt taacaaaccg ggtgagctga
    13081 atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg gtggggtata
    13141 tggtttcgcc gctgctgtgg aaaaagatcg ctcgtggtct gtctgccggt cgtgtgcagt
    13201 cggtggcggt ccgcctggtg gtagagcgtg agcgtgaaat taaagcgttc gtaccggaag
    13261 agttctggga agtcgatgcc agcacgacta cgccatctgg tgaagcgttg gagttgcagg
    13321 tgactcatca gaatgacaaa ccgttccgtc cggtcaacaa agaacaaact caggctgcgg
    13381 taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa ccgacaacca
    13441 gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc acccgtcttg
    13501 gttttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca ggctatatca
    13561 cttacatgcg taccgactcc actaacctga gtcaggacgc ggtaaatatg gttcgcggtt
    13621 atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag tacgccagca
    13681 aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat gtgatggcgg
    13741 aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc tggcgtcagt
    13801 tcgttgcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc gttggtgcgg
    13861 gcgatttccg cctgaaagca cgcggtcgta ttttgcgctt tgatggctgg acaaaagtga
    13921 tgcctgcact gcgtaaaggc gatgaagatc gtatcttacc ggcagtcgat aaaggcgatg
    13981 ctctgacgct cgttgaacta acaccagccc agcactttac caagccgcca gcccgtttca
    14041 gtgaagcatc gctggttaaa gaactggaaa aacgtggtat cggtcgtccg tctacctatg
    14101 cgtcgatcat ttcgaccatt caggatcgtg gctatgtgcg agtagaaaat cgtcgtttct
    14161 atgcggaaaa aatgggcgaa atcgtcaccg atcgccttga agaaaatttc cgcgagttaa
    14221 tgaactacga ctttaccgcg cagatggaaa acagcctcga ccaggtggca aatcacgaag
    14281 cagagtggaa agctgtactg gatcacttct tctcggattt cacccagcag ttagataaag
    14341 ctgaaaaaga tccggaagag ggtggtatgc gcccgaacca gatggttctg accagcattg
    14401 actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc gagtaccggg gtattccttg
    14461 gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt aacctggtgc
    14521 cggaaaacga agtgctgaac gtgctggaag gcgaagatgc tgaaaccaac gcgctgcgcg
    14581 caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc gatccgaaac
    14641 gtaagttgca tgtctgtggt aataacccaa cctgcgacgg ttacgagatc gaagagggcg
    14701 aattccgcat taaaggttat gacggcccga tcgttgagtg tgaaaaatgt ggctctgaaa
    14761 tgcacctgaa aatggggcgt ttcggtaaat acatggcctg caccaacgaa gagtgtaaaa
    14821 acacacgtaa gattttacgt aacggtgaag tggcaccacc gaaagaagat ccggtgccat
    14881 tacctgagct gccgtgcgaa aaatcagatg cttatttcgt gctgcgtgac ggtgctgccg
    14941 gtgtgttcct ggctgccaac actttcccga aatcgcgtga aacgcgtgcg ccactggtgg
    15001 aagagcttta tcgcttccgc gaccgtctgc cggaaaaact gcgttatctg gccgatgcgc
    15061 cacagcagga tccggaaggt aataagacca tggttcgctt tagccgtaaa accaaacagc
    15121 aatatgtctc ttcggaaaaa gacggaaagg cgactggctg gtcagcattt tatgttgatg
    15181 gcaaatgggt tgaaggaaaa aaataacctt taattctgtc aggtttttat aaacaaaggg
    15241 tcgcgaaagc ggcccttttt tattg
//
LOCUS       DAZPFP010000082        15153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPFP010000082 DAZPFP010000000
VERSION     DAZPFP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.082"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            167..1225
                     /gene="citC"
                     /locus_tag="ACHUR4_002420"
     CDS             167..1225
                     /gene="citC"
                     /locus_tag="ACHUR4_002420"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1455981.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1240..1536
                     /gene="citD"
                     /locus_tag="ACHUR4_002421"
     CDS             1240..1536
                     /gene="citD"
                     /locus_tag="ACHUR4_002421"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1455982.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1533..2441
                     /gene="citE"
                     /locus_tag="ACHUR4_002422"
     CDS             1533..2441
                     /gene="citE"
                     /locus_tag="ACHUR4_002422"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1455983.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2452..3984
                     /gene="citF"
                     /locus_tag="ACHUR4_002423"
     CDS             2452..3984
                     /gene="citF"
                     /locus_tag="ACHUR4_002423"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1455984.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3988..4538
                     /gene="citX"
                     /locus_tag="ACHUR4_002424"
                     /pseudo
     CDS             3988..4538
                     /gene="citX"
                     /locus_tag="ACHUR4_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="ACHUR4_002425"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="ACHUR4_002425"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1455985.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5442..6904
                     /gene="citT"
                     /locus_tag="ACHUR4_002426"
                     /pseudo
     CDS             5442..6904
                     /gene="citT"
                     /locus_tag="ACHUR4_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7018..7824
                     /gene="rna"
                     /locus_tag="ACHUR4_002427"
     CDS             7018..7824
                     /gene="rna"
                     /locus_tag="ACHUR4_002427"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1455986.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="ACHUR4_002428"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="ACHUR4_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1455987.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8601..8690
                     /gene="yldA"
                     /locus_tag="ACHUR4_002429"
     CDS             8601..8690
                     /gene="yldA"
                     /locus_tag="ACHUR4_002429"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1455988.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8694..9903)
                     /locus_tag="ACHUR4_002430"
                     /pseudo
     CDS             complement(8694..9903)
                     /locus_tag="ACHUR4_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10124..10552
                     /gene="uspG"
                     /locus_tag="ACHUR4_002431"
     CDS             10124..10552
                     /gene="uspG"
                     /locus_tag="ACHUR4_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1455989.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10673..12238)
                     /gene="ahpF"
                     /locus_tag="ACHUR4_002432"
     CDS             complement(10673..12238)
                     /gene="ahpF"
                     /locus_tag="ACHUR4_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1455990.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12483..13046)
                     /gene="ahpC"
                     /locus_tag="ACHUR4_002433"
     CDS             complement(12483..13046)
                     /gene="ahpC"
                     /locus_tag="ACHUR4_002433"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1455991.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13418..14164
                     /gene="dsbG"
                     /locus_tag="ACHUR4_002434"
     CDS             13418..14164
                     /gene="dsbG"
                     /locus_tag="ACHUR4_002434"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1455992.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14374..>15153
                     /locus_tag="ACHUR4_002435"
     CDS             14374..>15153
                     /locus_tag="ACHUR4_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1455993.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLN"
BASE COUNT         3703 a         3956 c         3735 g         3759 t
ORIGIN      
        1 aatttcacgc cgaaatactc cttaggatgt atagcaaaag gagaaaaaga tatacctcga
       61 tcacccccct ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat
      121 tcgttgcaaa tcgataacaa catgcacctt caggatacta tttattatgt tcggcaatga
      181 tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct
      241 gcatgaaaat gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga
      301 tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag
      361 tgaatccgtt cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc
      421 ctatgagcgg cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt
      481 ccgccagtgc ggtttttcca cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa
      541 cagcgccacg cgactgaaac gctatgccga atcgctgaaa aaatttcgtc atccagggaa
      601 caagattggc tgcattgtga tgaacgccaa tccctttacg aatggtcacc gttatctgat
      661 tcaacaagct gcggcacagt gcgactggtt gcatctgttt ttagtcaaag aagattcttc
      721 acgcttcccc tatgaagacc ggctggatct ggtgttaaaa ggcaccgccg atattccacg
      781 cctgactgtg catcgcggct cagaatacat catctcccgc gctacgttcc cttgctactt
      841 cattaaagaa cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg
      901 tcagtacctc gctcccgcgc ttggtgtaac tcaccgcttt gtcggtactg aacccttttg
      961 tcgcgttacc gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc
     1021 cgcagcgccc atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc
     1081 cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt
     1141 ccctgcagtc acgctgcatt atttgcagaa cctgcttgag cactcccgcc aggacgcggc
     1201 agctcgtcaa aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc
     1261 gttgcaggca cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag
     1321 gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc
     1381 accattctgg acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac
     1441 aaaggcgcac tggactgcat tttacatgca cgactggaag ccctgctggc acgcgccagc
     1501 ggtatcccgg ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta
     1561 aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca
     1621 actccttcat ctacccggct gatgccctga tgtttgacct cgaagactcc gtggcattac
     1681 gtgaaaaaga caccgcccgc cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg
     1741 atattgaaac cattgtgcgt gtcaacgcgc tggattccga atggggtgtt aacgacctgg
     1801 aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg
     1861 atgttctgga tattgaaaaa gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg
     1921 gcagcaccgg cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa
     1981 tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca
     2041 acctgcgtac agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt
     2101 tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca
     2161 acgaagccgg attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat
     2221 cgctgatcaa cccgcgtcag attgatctgc tgcacaacct ctacgcaccg acccagaaag
     2281 aagtggatca cgcccaccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg
     2341 gcgtggtttc cctgaacggc aagatggtgg acggtccggt tatcgatcgc gcccgtctgg
     2401 tgctctcccg tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag
     2461 aaaattgaac aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc
     2521 gatcttgccg ctttccagaa ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat
     2581 cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct caggtttaca ggacggcatg
     2641 acggtttcct tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac
     2701 gtcatcgcga agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc
     2761 catgcgccgc tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctctggc
     2821 ctacgcggtc cactggcgga agagatctcc cgtggtctgc tggcggaacc ggtgcagatc
     2881 cactctcacg gcggtcgtgt gcatctggta cagagcggcg aactgaatat cgacgtggct
     2941 ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca acggctacac cggtaaagcc
     3001 tgctgcggct ccctcggcta tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg
     3061 cttaccgaag aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt
     3121 gatttgatcg tcaaagttaa tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc
     3181 cgtatgacca ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc
     3241 aactctggct acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg
     3301 gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc
     3361 ctcggcggta ttaccgcgac gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg
     3421 ctggatgtgc agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac
     3481 atcgaaatca gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt
     3541 ctcgacgtgg tggtactgag cgcgctggaa attgacaggc agttcaacgt taacgtgctg
     3601 accggttctg acggcgtact gcgtggcgct tccggtggtc actgcgatac cgcgattgcc
     3661 tctgcgcttt ccatcatcgt cgcgccactg gtacgcggtc gtattccgac tctggtggat
     3721 aacgtgctga cctgcatcac cccgggctcc agtgtcgata ttctggtcac agaccacggt
     3781 atcgcagtta acccggcacg tccggaacta gcagaacgtc tgcaggaagc gggcattaaa
     3841 gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg
     3901 attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat
     3961 gttgtgcatc aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat
     4021 gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc
     4081 tggctcaagc accatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt
     4141 aaagacagcg aggtcacacg ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta
     4201 gccacaaagc agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg
     4261 gagggcatgt tgagcattgc cgccccggct cgcgacctca agctcgccac cattgagctt
     4321 gaacatagtc atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa
     4381 attctctccc gccgcgacta ttcactgcgc ctcgccgctg cctgttgtgc gagcaaagcg
     4441 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4741 gcctgtagtg cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4861 ttttctttag ggctgctatg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     4981 ctgcgtacca ataattcacg actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcaggt tatccactgg tgatcaacca cgccttgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5161 ctcctactga tggctaccaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 accgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctgct gatccttacc tgttttttag cacagattta attatttaag
     5401 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc
     5521 ccgacggtat gccgccgcag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggcctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5821 cccttttcct ggtgaaattc atggacaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attcccgaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc
     6121 cggtatccag attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt
     6181 gcttatcatt gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga
     6241 agaagtggca acctgggcgg gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga
     6301 gtggacgctg attggccttg tattgctcag cttaggtttg tgggtatttg gcagtgaagt
     6361 cattaatgct actgcggttg gtctgctggc agtttcgcta atgctggctc tgcacgttgt
     6421 gccgtggaaa gacattaccc gctataacag cgcatggaac acgctggtca acctggcaac
     6481 tctggttgtg atggctaacg gcctgactcg ttctggtttt attgactggt tcgccggtac
     6541 catgagtacg cacttggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt
     6601 gttctacttt gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc
     6661 ggttattctg gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct
     6721 gctggtgctg tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt
     6781 gattatttac ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt
     6841 cggggtgatt tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa
     6901 ctaatacgtt cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg
     6961 ggctttttta tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg
     7021 aaagcattct gccgtaacgc cgcgttgctc gcggtttctc tgcttccctt atcttctgcc
     7081 aacgccgtag cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc
     7141 tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa
     7201 tgtcgcctgc aaaccgaaac gaccaacaaa actgattttc tgaccgtaca tggtctgtgg
     7261 ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc
     7321 ggttgcgcta ctcgcccaat cccgaatcta ccagaagcac gcgccagccg aatgtgttca
     7381 tcgccggaaa ccgggttatc actggaaacg gccgctaaac taagtgaagt catgccagga
     7441 gctggcggac gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt
     7501 tttgatccgg acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc
     7561 gaagccggaa aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac
     7621 gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag
     7681 ggtaaccctg cgtatttgac tgaaattcag atctcgatca aagctgacgc catcaacgct
     7741 ccgctttctg caaactcatt cttgccacaa cctcacccag gtaactgtgg caaaaccttt
     7801 gtgattgata aagcgggtta ttaatcctct gatttacaaa acacgctaat tcgtcttcca
     7861 taaccatgcc gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8461 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     9001 gcgtagacgc ccggtacgct aacaatgccg caacgcttta ccgctgctgc cctccagttt
     9061 caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga cggcgtctat
     9121 tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg ccggatcgct
     9181 atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac gcaagcggta
     9241 gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac aggcgattgt
     9301 caacaatccc acaggaccag caccatagac tgcaacgctt gaaccttgtt ggatctgcac
     9361 attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg ctttatcatc
     9421 tgaaagcaaa ggcggtactt taaacggccc cacattccct ttcgggacgc ggacatattc
     9481 cgcctgcccg ccaggaacgc caccatacag gtgactataa ccaaacaatg ccgcgggagc
     9541 aggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt cgcaggcggc
     9601 atattgctgt aatcgacaga aaaaacagtc gccacaagca atgacgaacg gaattaccac
     9661 ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt tcaacgactt cccccataaa
     9721 ttcatgacca aaaatatcgc catgtttaac ctggggtatt ttgcctcgat aaagatggag
     9781 gtcagagcca cagatcgccg ttaccgtaat acgcagaata atatcatctg cctgttcaat
     9841 gcccggatcg ggaacatttt ctacctgaac gtgatgtggg ccgtgataag tcaatgcttt
     9901 catgcaacct ccagagacag ttcaaaaaag agtcagtctt taagggtagc tatcacagcg
     9961 ggaaaacggg cggtaccgca ggttttggga gaattcctag tgttcagtga gaatatatta
    10021 atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata aatattataa
    10081 tcatggagaa tgatttgttc atgattaaca gggagaaagg gttatgtata aggcaatcat
    10141 tatgccagtt gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc acgctgaatt
    10201 cctcgcccag gatgacggag ttattcatct acttcacgta ctacccgggt cagccagcct
    10261 gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc
    10321 agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc gcattaaaca
    10381 acatgtccgt tttggtagcg tgcgggatga agtcaatgag ttggcagaag aactgggggc
    10441 tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg acccatctgt taggttctaa
    10501 cgcctcgagc gtgatccgcc acgccaatct gcctgtactg gtcgttcgct aacagataaa
    10561 agtatttatg ccgccctgcc tggaacatcc cggcagggct ttttaatgcc ataaaaaagc
    10621 cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct tcttatgcag
    10681 ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct tcgccagtgg
    10741 cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac acgcctttga
    10801 cgttggtttc acatttcgca tcaatgataa tctcgcccat gcggttacgt tcgactgcgc
    10861 cttccagcca gttggtgttc ggcagcagac caatctggac aaaaataccg gccagttcga
    10921 tgttgtgaat attgccgctg acacgatctc gatattccag accaacgact ttgctgccgt
    10981 cgcctttcac ttccgtggtt tgcgcattca gaataatgtc gacgtttttc aggctgcgca
    11041 gtttgtcctg cagaacctgg tcggctttca tttctggcgc aaattccagc agcgttacgt
    11101 gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg ccgccgataa
    11161 ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt
    11221 tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca cccgtcgcca
    11281 cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg tgtagaccac
    11341 cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt
    11401 attcatcaac gtgaactttc agtgcgcctg ccaacttctg cccttcagtc ttcggtacag
    11461 aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt tcgcccatca
    11521 gaccggtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc gccgggccgg
    11581 aaccaacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc gcacgatttt
    11641 ccgcgccagt atcaattttg gcaacgattt cagtcaacgt catgcggccc tgaccaaact
    11701 ctttcccgtt tacgaacact gccggaacgc ccatcacgtt gcgatcggtg atttcgttct
    11761 ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg gttcaatacg ctcatcaggt
    11821 tcagcgcctg caccacgtcc gggcagttat ggcaagagag cgagtaatag gtttcgaatt
    11881 caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct ttcgacggat
    11941 gaccaccggt ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc agcggggagc
    12001 ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat caggaaagac ggcttacgca
    12061 ctggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg atttcagcca
    12121 acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt aactcaacag
    12181 gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg tcgagcatgt
    12241 ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc tggatgcagc
    12301 ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat taggtgaatt
    12361 tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg aatccccggg agcttacaca
    12421 agtaagtgac cggggtgagc gggcgacgcc aacgccgcta tggcgtgaaa gacgaaggaa
    12481 atttagattt taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat
    12541 ttagccgggc aaacttcacc tgggtgagaa gctacgtact gagctgcttt gattttacgc
    12601 agcagatcag acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc ctggatgata
    12661 ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg
    12721 tcgaagttac gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg
    12781 atggtttcag agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg
    12841 tatacgtcta cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc acccagttcg
    12901 gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg
    12961 gtatcttttt cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt
    13021 ttaattttgg tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag
    13081 gtaaccaatt tttgccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc
    13141 ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa
    13201 atggccccct ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg
    13261 cctgcaggct gaggtgttgc ccttacaaat gcaacaacga catggattac aacaccctca
    13321 taaacaaagg gcaatcacct gatctaagct cttacctatg acagtgatag gttatgcctt
    13381 ttactcgact tttgcactga ctgaaaagaa caaattaatg ttaaaaaaga tacttttact
    13441 ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct tccccagtaa aagcgattga
    13501 aaaacagggc attacaatca tcaaaacgtt cgatgccccc ggaggaatga aaggttatct
    13561 cggaaagtat caggatatgg gcgtcaccat ctacctgact cccgatggta agcacgctat
    13621 ctctggttac atgtacaacg agaaaggtga aaacctgagt aacacactta tcgaaaaaga
    13681 aatttacgca ccagccggac gcgaaatgtg gcaacggatg gaacaatccc actggctcct
    13741 cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc tccgatccgt tctgcccata
    13801 ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat tctggcaaag tgcaattaag
    13861 aacattgttg gttggggtta tcaagccaga aagcccggcg acagcagcgg caattcttgc
    13921 ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc tctggttgca agcttaagct
    13981 aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg ttaagtgaca atgagaaact
    14041 gatggacgat ctgggggcaa atgtcacgcc ggctatctat tacatgagta aggaaaatac
    14101 gctgcaacag gccgtggggt tgcccgatca gaaaacgctt aatatcatta tgggaaataa
    14161 ataaaaggta attcatggat ggttgaacta tatccctgct tttcaaccat cctaaaaaag
    14221 catgaatttc tcgctctacc ctaagtggct gtgctatttt agaaaaactt aatcctgagt
    14281 taagcaaaaa aacataatca ataaaaatac atacgcaaaa aacattgatt aagtgaatat
    14341 accatggaag aaaaatataa accggagtag tgtatggcca atctctacga cttgaaaaag
    14401 ttcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag cattagcaaa
    14461 gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca acgcttacgt
    14521 gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca
    14581 gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca aacaattaat
    14641 atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca gctcatttct
    14701 tgctctaata atagtatgct gatccgctgc ctacggcaag atgcctctgt cgagattgag
    14761 tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca ccgcaaagcc
    14821 gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat gccattgcat
    14881 acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga caatagcact
    14941 tatgaacaaa ttatggcaga agagttcacc caattaatag ccaaatcagc aggagttgat
    15001 gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt tcgtggttcc
    15061 tctttgctga caataattaa tagcattgca gttacagatt tactgggaat agtcccctat
    15121 gaactttata actaccaccg tgactttctt aat
//
LOCUS       DAZPFP010000083        15128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPFP010000083 DAZPFP010000000
VERSION     DAZPFP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.083"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUR4_002436"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUR4_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1455994.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUR4_002437"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUR4_002437"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1455995.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHUR4_002438"
     CDS             1531..2478
                     /locus_tag="ACHUR4_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR1455996.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHUR4_002439"
     CDS             2548..3354
                     /locus_tag="ACHUR4_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1455997.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHUR4_002440"
     CDS             3364..3771
                     /locus_tag="ACHUR4_002440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1455998.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHUR4_002441"
     CDS             3771..4265
                     /locus_tag="ACHUR4_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR1455999.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHUR4_002442"
     CDS             4331..5128
                     /locus_tag="ACHUR4_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1456000.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHUR4_002443"
     CDS             5139..5927
                     /locus_tag="ACHUR4_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR1456001.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUR4_002444"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUR4_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR1456002.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUR4_002445"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUR4_002445"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1456003.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHUR4_002446"
     CDS             complement(8370..10001)
                     /locus_tag="ACHUR4_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1456004.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUR4_002447"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUR4_002447"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1456005.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUR4_002448"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUR4_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1456006.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3598 a         3990 c         3942 g         3598 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aactttta
//
LOCUS       DAZPFP010000084        15043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPFP010000084 DAZPFP010000000
VERSION     DAZPFP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.084"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHUR4_002449"
     CDS             complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHUR4_002449"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1456007.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2618..3568
                     /gene="tal"
                     /locus_tag="ACHUR4_002450"
     CDS             2618..3568
                     /gene="tal"
                     /locus_tag="ACHUR4_002450"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1456008.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3588..5591
                     /gene="tkt"
                     /locus_tag="ACHUR4_002451"
     CDS             3588..5591
                     /gene="tkt"
                     /locus_tag="ACHUR4_002451"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1456009.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5694..6737)
                     /locus_tag="ACHUR4_002452"
     CDS             complement(5694..6737)
                     /locus_tag="ACHUR4_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1456010.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHUR4_002453"
     CDS             complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHUR4_002453"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1456011.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHUR4_002454"
     CDS             complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHUR4_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1456012.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9691..11408
                     /gene="narQ"
                     /locus_tag="ACHUR4_002455"
                     /pseudo
     CDS             9691..11408
                     /gene="narQ"
                     /locus_tag="ACHUR4_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11610..14723
                     /gene="acrD"
                     /locus_tag="ACHUR4_002456"
     CDS             11610..14723
                     /gene="acrD"
                     /locus_tag="ACHUR4_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1456013.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHUR4_002457"
     CDS             complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHUR4_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1456014.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3529 a         3998 c         3929 g         3587 t
ORIGIN      
        1 aaaccgctta ccctgaatat tcagggtaag cgtgagagtt aagaatgaat tacagcggtt
       61 gggtttgcgc ttcaaccacg gccagcgcca ccatattgac gatacgacgc accgatgcga
      121 tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac
      181 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
      241 tcaccagaat attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg
      301 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagatct ggcgcacgat
      361 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
      421 agttggagtg cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg
      481 ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat
      541 cggcaataaa ggtgttacca ctcggcagca gcagcgcatt cattgcacct gcggtgtgaa
      601 cgccatcgcg ataaccaaag acatttttca ccacactaaa atgttcatga taatcgccca
      661 ccgtaccgca gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg
      721 tcgggttact gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga
      781 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgattt
      841 caaaatcaac gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
      901 tcggacgacc gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat
      961 gcagaacgcg cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct
     1021 gggagaaaat cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga
     1081 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgcttttg
     1141 cgaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     1201 ccggaccaaa gctcagatcc tgaacgccat acgctgaagc caccacttca ctctgttctg
     1261 catgggccag ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg
     1321 ttgcgccaac gtccagcgcg ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct
     1381 ggttcgggta gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca
     1441 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggt gcccgagcca
     1501 ttttcttcac catttcctgg gtcagcactt tcgggccgga acagcccagg aaaatatccg
     1561 caccttcaat cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt
     1621 tggtttccgc catatttggc tcacggccct gatagataac gccttttgaa tcgcaaacca
     1681 cgatgttatg tttttgcagc cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     1741 cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc
     1801 cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg
     1861 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     1921 cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc gacgacttca ataaatttgt
     1981 ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaata
     2041 gaacgccctt gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca
     2101 gcaccgccgt accgttagag atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg
     2161 cttttagcga gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     2221 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     2281 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     2341 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     2401 acattattac cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata
     2461 taatgttttg gtaataatcc tataacactg atgttacctg cttaatccag caataccatg
     2521 cctgtctgct atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc
     2581 actcatctaa cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa
     2641 acagttcacc actgtcgtgg cagacagctg cgatattgag tccattcgcc attatcatcc
     2701 ccaggatgcc accactaatc cttcgctgtt actcaaggct gccggattat cacaatatga
     2761 gcatttaata gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca
     2821 ggtggtcgca gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt
     2881 acccggttgc gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat
     2941 tgagaaggcg cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat
     3001 tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga
     3061 aggtattaac tgcaacctga cgctgctgtt ttcttttacc caggcgcggg cctgtgcgga
     3121 agcaggcgtt tttctgattt cgccgtttgt agggcgtatt tatgactggt atcaggcacg
     3181 caagccgatg gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat
     3241 ctacgactac tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg
     3301 caccgaacaa atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct
     3361 gaaggagctg caggaaaaag tttctccagt ggtacgtaaa ttaatcccgc cttctcagac
     3421 gttcccacgc ccagcaccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc
     3481 gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact
     3541 ggaagatctt cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag
     3601 accttgccaa tgcgattcgc gcactcagta tggatgctgt acaaaaagcc aattctggtc
     3661 atcccggcgc gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta
     3721 aacataatcc taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc
     3781 acgcgtcgat gctgctctac agtttgctac atctgaccgg ttacgacctg ccgctggaag
     3841 aactgaagaa cttccgtcag ttgcattcga aaaccccagg ccacccggag attggctata
     3901 cgccaggcgt tgaaaccacc accggcccgc ttggacaagg tttggcgaac gccgtcgggc
     3961 tggcgatagc agagcgtaca ctggcggcgc agtttaacca gccagaccat gagatcgtcg
     4021 atcacttcac ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag
     4081 tctgttcgct ggcaggcacg ctgggactag gcaagctgat tggtttttac gatcacaacg
     4141 gtatttccat cgacggtgaa acagaaggct ggtttaccga cgatacggca aaacgttttg
     4201 aagcctatca ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg
     4261 aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg
     4321 tgattggctt tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg
     4381 gggaagaaga agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga
     4441 tccctaaaga gatttatcac gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga
     4501 gctggaatga gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagtttc
     4561 cccgacggat gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca
     4621 atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta
     4681 acgcttacgg gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca
     4741 acctgaccat ctggaaaggt tctgtttctc tgaaggaaga tccggcgggc aactacattc
     4801 actacggggt gcgtgaattt ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg
     4861 gctttgtgcc gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc
     4921 ggatggcggc actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc
     4981 tgggcgaaga tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc
     5041 caaatttcag cacctggcga ccgtgcgatc agatggaagc ggcggtgggc tggaagctgg
     5101 cggttgagcg ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg
     5161 tggaacgtac gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca
     5221 gcggcggtaa gccagatatt attctgattg ccaccggttc agagatggaa attaccctgc
     5281 aagcggcaga gaaattagca ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga
     5341 ccgatatttt cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg
     5401 cggctcgcgt ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga
     5461 aaggggcaat tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc
     5521 cgttctttgg ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga
     5581 aaggtgcctg atggtgatcg ccggatgctg tatgttcccc tcaccctaac cctctcccca
     5641 gaagggcgag gggacggtcc gtgcgctttt tagggccgca tctgcgattt tgtttaacgg
     5701 gtgatccaca acgtgggcct ggcgtctggc ccatgccagt tatcgcaggt aggttctgcg
     5761 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
     5821 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
     5881 gcgttcatca gttccagttc attcgtctcc tggccgttgt tgaacggcaa acgcaaccga
     5941 accgggcgcg aggctagcgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
     6001 taggcccctg cctcacagct aatcatcatc agtgctttat catcggtcag cgcagtgaca
     6061 ttcacctcac gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
     6121 taatccagca aatcgttacg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
     6181 gcgacctctt tcagcgcagg cgcgggcggt acgctgagag gcggttcgtc ccctttcttg
     6241 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct
     6301 tttaagccgc tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc
     6361 tgaattatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
     6421 gaaatccgcc acttgtcacc acttaatgcc aacggctcac catctaatag cagccgtggc
     6481 gctatctccc cttcagacgc atccggcgac ttcaacccac cgcgctcaat acgtaaaacg
     6541 gcatcggtat gcgccccagc gctgcggctc agggtcatca ccagtccatt atgatcgccc
     6601 gtattgcgcg ccacacaaaa attttgatta ttacaggtga cctgccagtc ggaaaacgcc
     6661 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
     6721 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
     6781 atcttcgttg cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac
     6841 aacgaaatat tattgctatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca
     6901 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
     6961 acggcagctc gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct
     7021 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca
     7081 tatacagttc aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt
     7141 ctttacgaat gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
     7201 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
     7261 cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt
     7321 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca
     7381 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
     7441 cgactccaca gcgcgaaatg aacaattata ttacgctgtg aagtcgggtt tgtctgcgca
     7501 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
     7561 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg
     7621 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
     7681 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg
     7741 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt
     7801 acaggtcgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatt
     7861 ccgcaaacgt gaccttgctc attcagctca agcgccaccg gctggacgtt aaattcgaag
     7921 ttggccccct cttcgcgggc gttcttcacc tctttcttcg agcctggcat gttagcttca
     7981 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
     8041 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac
     8101 ggctcttccg gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc
     8161 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa
     8221 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcaaatccaa agagacatct
     8281 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
     8341 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
     8401 atttctggat ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca
     8461 caggccagcc ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca
     8521 tgacttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaatg
     8581 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
     8641 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
     8701 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc
     8761 agcgggcagg tccattcaca aacgctgtgc tcgccgaact taaggcagcg cgaggcttcc
     8821 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
     8881 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
     8941 ctcattaccg gcatttcttg tgcagcggta ctggcatgcc acggttgatg ctcctgacgt
     9001 gcggtgcgca agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
     9061 agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg
     9121 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
     9181 atcgtaccaa acggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
     9241 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
     9301 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gctgatgttt gataaccgta
     9361 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
     9421 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
     9481 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
     9541 acccgactta caggggaacc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
     9601 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaactggaa
     9661 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
     9721 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
     9781 ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctgcgt
     9841 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
     9901 cgccagttgt ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat
     9961 gtgccagaag cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat
    10021 aatcggctca gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat
    10081 cagatagacc tgttcgtgct ggctttacag cactacgctg aacgcaaaat gctgctggtg
    10141 gtggcgattt ccctggctgg cggtatcggt attttcacgc tggtcttttt tactttgcgc
    10201 cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt
    10261 gaacacgggc agttcgactc gccgccgctg gataccagtc tgccgaatga gcttggtctg
    10321 cttgcaaaaa cctttaacca aatgtcgagc gagctgcata aattgtacct ttcgctggag
    10381 gcgtcagtag aagaaaagac ccgcgatctc cacgagacca agcgtcgtct ggaggtgttg
    10441 tatcagtgca gtgttcccag gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc
    10501 atattttgca gattgttcgc gacaatgaag cagctgaata tctggagctg aacgttgggg
    10561 ataactggcg gattagcgaa gggaagccaa accctgagct gccaatgcag attttaccgg
    10621 tgacaatgca agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat
    10681 cagaaccgct gcttaacagt gtttcatcga tgctgggacg cggtttgtac tttaatcagg
    10741 cgcagaagca ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat
    10801 tgcacgactc gctggctcag gtgctttctt acttacgtat ccagttgacg ttactgaagc
    10861 gttcgatacc ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt
    10921 tgaatgatgc ttatcggcag ttacgcgagc tgttgactac tttccgcctg acgctgcagc
    10981 aggcggatct cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg
    11041 ctaaactgac cctcgactgc cgtctgccaa ccctggcact ggatgcgcaa atgcaggtgc
    11101 atttgttgca aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg
    11161 aaatcgccgt cagttgcgtc accgcgccgg acggcaatca cacggtttat atccgtgata
    11221 acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca ttatggactg aatatcatgc
    11281 gcgaacgcgc ggaacggctg ggcgggacgc tgactttttc acaaccttcc ggcggcggca
    11341 cgttagtgag tattagcttt cgctccgcag agggtgaggg gaaggggaac acgccggctg
    11401 aggtgtaaat aattaccctc acaccggccc tctcccttta aaggcgagag ggaaaaacgt
    11461 gcccatattg acctgtaagc gagtgtgtca gcaatctcat gataatttac attaactcct
    11521 ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac
    11581 aatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt
    11641 ttgcctgggt gctgacaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc
    11701 ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg
    11761 gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg
    11821 gcctcgataa tctgatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca
    11881 ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc
    11941 tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta
    12001 aaaccggcga taccaacatt ctgaccattg ccttcgtctc taccgatggc tcgatggata
    12061 aacaggatat tgctgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg
    12121 gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg
    12181 cgaaactcaa cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga
    12241 acgcgcagat tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca
    12301 acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct
    12361 tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg
    12421 gggcggagaa atacgattat cttagccgct tcaatggtaa gccagcctcc gggttggggg
    12481 taaaactggc ctccagcgca aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg
    12541 acgagctggc gcagtatttc ccgcacggac tggaatacaa ggtggcgtat gaaaccacct
    12601 cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg
    12661 ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg
    12721 ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca
    12781 acaccttaac catgttcgcg atggtgctgg cgatcggtct gctggtggat gacgccatcg
    12841 tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca
    12901 cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg
    12961 cggtatttgt accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct
    13021 ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgctc ctcactccgg
    13081 ctctgtgtgc cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggtt
    13141 tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg
    13201 cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca
    13261 tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cggggcatgt
    13321 ttactacctc ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg
    13381 agcaaatcga gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca
    13441 ccgttggttc tggccctggg ggtaacgggc aaaacgtggc gagaatgttt atccgcctga
    13501 aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa
    13561 cgaaggcgtt taaccaaatt aaagaagctc gcgttatcgc cagcagccca ccagcaatta
    13621 gcggtcttgg tagttctgca ggttttgata tggagttgca ggaccacgct ggagcgggtc
    13681 acgatgcgct gatggcagca cgtaatcagt tgctggcgct ggcggcggaa aacccggagc
    13741 taacccgtgt gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc
    13801 agcgtaaagc tcaggcgctg ggcgttgata tcgacgatat taacgacaca ctgcaaaccg
    13861 cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg
    13921 tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa
    13981 ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gaaacaggct
    14041 cgccgcgtct ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac
    14101 cgggggtcag taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa
    14161 acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg
    14221 ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtctggcg gcattgtatg
    14281 aaagctggtc ggtaccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc
    14341 tgctggcaac ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa
    14401 cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga
    14461 accaaaaagg ccatgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc
    14521 cgattctgat gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg
    14581 gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag
    14641 ctactattct ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc
    14701 cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcctttt
    14761 tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttatactt tgtctcagga
    14821 attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca
    14881 taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa
    14941 tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc
    15001 ctgcggtgat aatttgtatg tgatacacag caacatttcg aga
//
LOCUS       DAZPFP010000085        15043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPFP010000085 DAZPFP010000000
VERSION     DAZPFP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.085"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            164..649
                     /locus_tag="ACHUR4_002458"
     CDS             164..649
                     /locus_tag="ACHUR4_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456015.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACHUR4_002459"
     CDS             646..1032
                     /locus_tag="ACHUR4_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456016.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACHUR4_002460"
     CDS             1057..3270
                     /locus_tag="ACHUR4_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR1456017.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3293..3738
                     /locus_tag="ACHUR4_002461"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACHUR4_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACHUR4_002462"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACHUR4_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACHUR4_002463"
     CDS             7538..7741
                     /locus_tag="ACHUR4_002463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456018.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACHUR4_002464"
     CDS             7759..8079
                     /locus_tag="ACHUR4_002464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456019.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACHUR4_002465"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACHUR4_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUR4_002466"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUR4_002466"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1456020.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUR4_002467"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUR4_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1456021.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUR4_002468"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUR4_002468"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1456022.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUR4_002469"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUR4_002469"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1456023.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACHUR4_002470"
     CDS             13395..14162
                     /locus_tag="ACHUR4_002470"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1456024.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUR4_002471"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUR4_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1456025.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         3782 a         3720 c         4082 g         3459 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccccgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaa
//
LOCUS       DAZPFP010000086        14978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPFP010000086 DAZPFP010000000
VERSION     DAZPFP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.086"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHUR4_002472"
     CDS             complement(<1..104)
                     /locus_tag="ACHUR4_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456026.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLN"
     gene            complement(520..1461)
                     /gene="fabY"
                     /locus_tag="ACHUR4_002473"
                     /gene_synonym="yiiD"
     CDS             complement(520..1461)
                     /gene="fabY"
                     /locus_tag="ACHUR4_002473"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1456027.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="ACHUR4_002474"
     CDS             complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="ACHUR4_002474"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1456028.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1940..2812)
                     /locus_tag="ACHUR4_002475"
     CDS             complement(1940..2812)
                     /locus_tag="ACHUR4_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1456029.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="ACHUR4_002476"
     CDS             complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="ACHUR4_002476"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1456030.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3504..4289)
                     /locus_tag="ACHUR4_002477"
     CDS             complement(3504..4289)
                     /locus_tag="ACHUR4_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1456031.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="ACHUR4_002478"
     CDS             complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="ACHUR4_002478"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR1456032.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5387..6283
                     /gene="yihU"
                     /locus_tag="ACHUR4_002479"
     CDS             5387..6283
                     /gene="yihU"
                     /locus_tag="ACHUR4_002479"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1456033.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6307..7185
                     /gene="yihT"
                     /locus_tag="ACHUR4_002480"
     CDS             6307..7185
                     /gene="yihT"
                     /locus_tag="ACHUR4_002480"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR1456034.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7202..8443
                     /gene="yihS"
                     /locus_tag="ACHUR4_002481"
     CDS             7202..8443
                     /gene="yihS"
                     /locus_tag="ACHUR4_002481"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR1456035.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8581..9483
                     /locus_tag="ACHUR4_002482"
     CDS             8581..9483
                     /locus_tag="ACHUR4_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR1456036.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9682..11718
                     /gene="yihQ"
                     /locus_tag="ACHUR4_002483"
     CDS             9682..11718
                     /gene="yihQ"
                     /locus_tag="ACHUR4_002483"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR1456037.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11764..13138
                     /locus_tag="ACHUR4_002484"
                     /pseudo
     CDS             11764..13138
                     /locus_tag="ACHUR4_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13181..14584
                     /locus_tag="ACHUR4_002485"
     CDS             13181..14584
                     /locus_tag="ACHUR4_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456038.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14652..>14978
                     /locus_tag="ACHUR4_002486"
     CDS             14652..>14978
                     /locus_tag="ACHUR4_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR1456039.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3673 a         3855 c         3736 g         3714 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa gcgggatatg
      961 ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag
     1021 agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat
     1081 ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct
     1141 acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca tcgccatcag
     1201 cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa tggacgcttc
     1261 attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct cgtcgacgac
     1321 catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag
     1381 gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt cttctgtttg
     1441 tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag
     1501 ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata
     1561 tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa
     1621 tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc
     1681 ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact gccgcccgcc
     1741 tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagcaca
     1801 cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc
     1861 aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct ggcacgggtt
     1921 acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct gcttgtttta
     1981 gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg
     2041 tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa atgagctgat
     2101 atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagtg
     2161 cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc
     2221 tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc agcacgttgt
     2281 cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa ctgatcgcca
     2341 gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcgaacgagt
     2401 aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt
     2461 acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc atcttgttgg
     2521 aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca ggcagaaagt
     2581 tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga aaagcggcga
     2641 aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt
     2701 tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt tttagccagg
     2761 cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc ataacacctt
     2821 cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa tgcctagctg
     2881 attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa
     2941 accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca tccccagatc
     3001 ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt ccggccagaa
     3061 ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct gctcacgcag
     3121 tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct gccagccgtg
     3181 agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct ctgcgaacgc
     3241 ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat gaaaactctt
     3301 cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca cacggttaaa
     3361 gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg
     3421 cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc aaggtatgaa
     3481 gccaggttaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat ttcaatttgg
     3541 ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa gagcgcagtg
     3601 acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg
     3661 accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc ttcgtttaca
     3721 tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac cagataatca
     3781 gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct attatgagag
     3841 cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag aatatgcgcc
     3901 acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc ccgggcaaca
     3961 tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat atagtctgcc
     4021 accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg ctcgaacgcc
     4081 gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc
     4141 tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag cagatttgcc
     4201 agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc
     4261 gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat caccctggca
     4321 ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat cccggctcgt
     4381 ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac tgactccgct
     4441 aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc tcccgcacct
     4501 gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc attttccagc
     4561 cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag cgtttgtgcc
     4621 tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc cggttctgaa
     4681 aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt aatgtccccg
     4741 tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt agcgccgtcg
     4801 tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc ctccaaccac
     4861 tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc gcctttggta
     4921 tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt gtaacgggtg
     4981 ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc atcacctacg
     5041 cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc cgtcgccgct
     5101 ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc cgtcggtaac
     5161 ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac acgaatcatg
     5221 gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt ttaaaaagtg
     5281 acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac aagcaaagca
     5341 tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg cagcaatcgc
     5401 gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc agcaagggca
     5461 tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag acaaaggtgc
     5521 gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca ttaccatgtt
     5581 gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt
     5641 atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa ccgataaatt
     5701 gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag gccgtacttc
     5761 tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg aacaagttga
     5821 acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg gcggtccggg
     5881 catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca atgcgctttc
     5941 ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt
     6001 gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa acaaagtcct
     6061 cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc ttggcatcgc
     6121 ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt
     6181 ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca
     6241 ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg ataaaggaat
     6301 aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg ttttgccatg
     6361 ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg ggcacccgct
     6421 ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc cctctcgcct
     6481 tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt tgagcaaaac
     6541 gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc tggcaacggt
     6601 attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat caaacaagac
     6661 ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt
     6721 ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt aagcatcatt
     6781 gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc
     6841 gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga aatgccgctt
     6901 tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa tgaccatatc
     6961 aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc gcgtgccgta
     7021 cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg
     7081 gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc gaaattacaa
     7141 caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga ggataaaagg
     7201 aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa ccgaccgcat
     7261 ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag gcaataaagg
     7321 gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt tgcacgttta
     7381 ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc acggtattaa
     7441 agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct gcgtgaatga
     7501 cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc
     7561 cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt acaccattga
     7621 gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt cctgggacga
     7681 agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc
     7741 tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt
     7801 ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta acgaacactt
     7861 cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc atcgcttccg
     7921 cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc tgcacatcca
     7981 tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag atgccaaagg
     8041 tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg ggattgttta
     8101 taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga
     8161 agcaatgggt acggtctacg cgctctacac cgtcactggc gatcgccagt atgaaacctg
     8221 gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa atggttcctg
     8281 gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg gcaaacagga
     8341 tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc
     8401 tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct gaaaataggg
     8461 tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccggtgca
     8521 gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa ggtgccttgt
     8581 atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
     8641 cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca gggttgtcat
     8701 ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa agtgcttatt
     8761 ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga atatcagtta
     8821 cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg gcgagactgg
     8881 caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc gcccagttac
     8941 ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt
     9001 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg cgtgggtatt
     9061 catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
     9121 aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca cgttgatgag
     9181 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
     9241 aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc gctttcggta
     9301 agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acagcgtcag
     9361 ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg tatcgactta
     9421 ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg acagagaaaa
     9481 taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca
     9541 ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt ttttatatat
     9601 cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac aatctcgttc
     9661 tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga ttttcaattt
     9721 catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt aacccataac
     9781 aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat gttccgcggt
     9841 aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc catcgtcagc
     9901 cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag cgccacgctg
     9961 aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga caaccttaac
    10021 cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta cggctgcggc
    10081 gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa
    10141 ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa agaaaatgcg
    10201 ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac gcagaagtat
    10261 tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc ggaataccat
    10321 gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga cacatacatc
    10381 tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc cgactggatt
    10441 tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa gaaactggac
    10501 accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg gtctgggatc
    10561 cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag cgaaaactac
    10621 ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt cctggcctat
    10681 atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca acacggctat
    10741 ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt ttacggcggc
    10801 gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat caaaaagaac
    10861 atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct gcccaccgac
    10921 acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc tgcgctgtgg
    10981 gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat cctcttcttt
    11041 atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc gggcgaccag
    11101 aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc gctgtcgctg
    11161 gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac cctgtttgag
    11221 atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg
    11281 atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg cgatgcggaa
    11341 actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc ttatctgaaa
    11401 gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct gttcctgcat
    11461 tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg ccgcgacatt
    11521 ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct gccggaggat
    11581 aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac cgttaatgcg
    11641 cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc actgttcgcg
    11701 tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa agggagaaca
    11761 tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt taaagagaaa
    11821 ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg tacgctttat
    11881 cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg cattatcttt
    11941 ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat gttggattcc
    12001 cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc gtcattcccg
    12061 gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg
    12121 gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat gaactgctcc
    12181 tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc actggcggca
    12241 tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt cgtgccagtt
    12301 atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac gctgttttca
    12361 ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa
    12421 acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc
    12481 ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg ggcgtttaac
    12541 gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc catcctgttg
    12601 tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct gatgcctggc
    12661 gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg
    12721 ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc gttctcctgc
    12781 ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg
    12841 cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca ccttctttcg
    12901 caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaattggcta
    12961 tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt tgatcttcat
    13021 ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct acagcctgaa
    13081 cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca cggcgtaata
    13141 ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact
    13201 gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa
    13261 tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg aattagggat
    13321 gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg cgtttaccga
    13381 tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg
    13441 tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc agtttatcgc
    13501 caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta tgatgtttgg
    13561 actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa
    13621 cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt
    13681 gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta
    13741 tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc tgtgctacag
    13801 aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca gtattctcaa
    13861 atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg ctaacctgtg
    13921 tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct
    13981 gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg
    14041 cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg
    14101 catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta actctttcac
    14161 tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca gcctgaactg
    14221 ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg
    14281 ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt
    14341 gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac
    14401 attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc
    14461 attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga ttattgagga
    14521 aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt ccgtaacgtt
    14581 ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa
    14641 atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg acctctgcct
    14701 cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc tccgatcagg
    14761 gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt atgttaacca
    14821 atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa attgaaggtt
    14881 ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc ttaattaatg
    14941 ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAZPFP010000087        14948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPFP010000087 DAZPFP010000000
VERSION     DAZPFP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.087"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            100..342
                     /gene="yjbE"
                     /locus_tag="ACHUR4_002487"
     CDS             100..342
                     /gene="yjbE"
                     /locus_tag="ACHUR4_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1456040.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            456..1094
                     /locus_tag="ACHUR4_002488"
     CDS             456..1094
                     /locus_tag="ACHUR4_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1456041.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1091..1828
                     /locus_tag="ACHUR4_002489"
     CDS             1091..1828
                     /locus_tag="ACHUR4_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1456042.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1828..3924
                     /locus_tag="ACHUR4_002490"
     CDS             1828..3924
                     /locus_tag="ACHUR4_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1456043.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3971..4249)
                     /locus_tag="ACHUR4_002491"
     CDS             complement(3971..4249)
                     /locus_tag="ACHUR4_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456044.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4250..4393
                     /locus_tag="ACHUR4_002492"
     CDS             4250..4393
                     /locus_tag="ACHUR4_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456045.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4519..4929
                     /gene="psiE"
                     /locus_tag="ACHUR4_002493"
     CDS             4519..4929
                     /gene="psiE"
                     /locus_tag="ACHUR4_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1456046.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHUR4_002494"
     CDS             complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHUR4_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1456047.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHUR4_002495"
     CDS             complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHUR4_002495"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1456048.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHUR4_002496"
     CDS             complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHUR4_002496"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1456049.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHUR4_002497"
     CDS             complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHUR4_002497"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1456050.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10977..12092
                     /gene="malK"
                     /locus_tag="ACHUR4_002498"
     CDS             10977..12092
                     /gene="malK"
                     /locus_tag="ACHUR4_002498"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1456051.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12164..13504
                     /gene="lamB"
                     /locus_tag="ACHUR4_002499"
     CDS             12164..13504
                     /gene="lamB"
                     /locus_tag="ACHUR4_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1456052.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13656..14576
                     /gene="malM"
                     /locus_tag="ACHUR4_002500"
     CDS             13656..14576
                     /gene="malM"
                     /locus_tag="ACHUR4_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1456053.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14805..>14948
                     /locus_tag="ACHUR4_002501"
     CDS             14805..>14948
                     /locus_tag="ACHUR4_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456054.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDY
                     FIYI"
BASE COUNT         3708 a         3836 c         3919 g         3485 t
ORIGIN      
        1 ttccccgcgc cgtaattcgc atgctttagt tgtgtatact cgatcccgcc cgaaatgttt
       61 ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc
      121 atttttgcca tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc
      181 gaagcggcag agtcggcagc aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc
      241 agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct
      301 aataccggga ccaccacaac cacgaccacc agtacccagt aataaagtat gtatccccaa
      361 aataatttga gtcattgcat ctgtggcttg aagtatgaag ggatttacca taaccacact
      421 ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa gcgacctgca ctcattctta
      481 tctgcctgct attacaggcc tgttcagcca cgactaaaga gctgggcaat tcactgtggg
      541 acagtctgtt cggcacgcca ggcgtacagc tgacggacga tgatattcaa aatatgccct
      601 acgccagcca gtacatgcag cttaatggcg ggccgcaatt gtttgtggtg ctggccttcg
      661 ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc cactcttgtc acacaacatg
      721 gtcgtctggt gaagactttg cttggcggcg acaacctgat tgaagtgaat aaccttgccg
      781 ccgacccgct gattaaaccc gcacaaattg ttgatggtgc aacatggacg cgcacgatgg
      841 gctggaccga gtaccagcag gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg
      901 gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt tcgcgtgctg gacgaagaag
      961 tctccaccga ccaggcgcgc tggcataacc gctactggat cgacagcgaa gggcaaattc
     1021 gccagtcgga acagtatctc ggcgcggatt atttcccggt gaaaaccact ctcatcaagg
     1081 cggcaaaaca atgattaaac aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc
     1141 ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct
     1201 aacgggcgca gagcatttaa tcgatctggt agggcaacct cgactggcaa acagctggtg
     1261 gcctggtgcg gtgattagcg aagagctggc aacggcggca gcattgcgtc agcagcaggc
     1321 gttgctgaca cggctggcag aacagggcgc agattccagc accgacgatg ccgccgccat
     1381 taacgcctta cgccagcaaa tccaggcatt gaaagtgaca ggcaggcaaa aaatcaatct
     1441 tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg ccgttgcagg gcaactacac
     1501 gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc gggcttatca gccatcctgg
     1561 caatcagcca ttcactccag gtcgcgacgt ggcgagctat ctctctgacc agagcctgct
     1621 cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg gacggacgca cgcaaaaagc
     1681 gccggtggct tactggaaca agcgtcacgt agagccgatg cccggcagca ttatttatgt
     1741 tggcctcgca gattccgtct ggagtgagac gcctgatgcc cttaacgccg acattcttca
     1801 gactctgacg cagcggatac cccaataatg aaaaaaagac atctgcttag cttactggcg
     1861 ctgggcatta gcacagcttg ctacggcgaa acatatcctg cgcccattgg tccgtcgcag
     1921 tcggatttcg gtggcgtagg attattacaa actcccaccg cgcgtatggc acgggaaggg
     1981 gagttgagtc tgaactatcg cgataacgat cagtaccgtt attactcagc ttcagtgcaa
     2041 ctcttcccgt ggctggaaac aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc
     2101 agcgtcgaag cgttctctgg cgatcaaacg tataaagata aagccttcga tctcaaactg
     2161 cgtttgtggg aagagagtta ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc
     2221 ggtacggggc tgtttgatgc ggaatatctt gttgccagca aagcctgggg gccgttcgat
     2281 tttacgctcg gcctgggctg gggatatctg ggcaccagcg gtaatgtaaa aaatccgctc
     2341 tgttcagcca gtgataaata ttgctatcgc gataacagct acaaacaggc gggatctatc
     2401 gacggtagcc agatgttcca cggtcctgcc tcactgtttg gcggcgtgga ataccagacg
     2461 ccctggcaac cgctgcgtct gaaactggag tatgaaggta ataattatca gcaggatttt
     2521 gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg cgatttatcg cgttaccgat
     2581 tgggccgacg ttaaccttag ctatgaacgt ggcaacacct ttatgtttgg cgtcacgctg
     2641 cgcaccaact ttaacgatct gcgcccgtcc tacatcgata acgcccgccc gcaatatcaa
     2701 ccgcagccgc aggatgccat tttgcagcat tcggtggtgg cgaatcagtt aacgttgctg
     2761 aaatacaacg ccggacttgc cgatccgcag atccaggcga aaggcgatac gctgtatgtt
     2821 accggcgagc aggtgaaata tcgtgattcg cgcgaaggga tcatccgtgc taatcggatc
     2881 gtgatgaacg atctgccgga tgggatcaaa acgatccgca ttacggaaaa tcgccttaac
     2941 atgccgcaag tgacgacgga aaccgatgtc gccagcctga aaaatcatct cgccggagag
     3001 ccgttgggcc acgaaacgac gctggcgcac aaacgcgtcg agccagtggt tccgaagtcc
     3061 accgagcagg gctggtatat cgacaaatca cgctttgatt tccatatcga tccggtgctg
     3121 aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc agctgggcgt gatgggaacg
     3181 gcagatttgt ggctgacgga ccatctactg accaccggca gcctgtttgc aaatcttgcc
     3241 aacaactacg acaagtttaa ctacaccaat ccaccgcagg actcgcactt accgcgcgtt
     3301 cgtacccatg tgcgcgagta tgtgcagaac gatgtctatg tgaataacct gcaagccaac
     3361 tacttccagc atctgggcaa cggcttctac ggtcaggtct atggaggtta tctcgaaacc
     3421 atgtttggcg gtgcgggggc cgaagtgttg tatcgtccgc tggacagtaa ctgggcgttt
     3481 ggtctggatg ccaactacgt taaacagcgc gactggcgta gtgcaaaaga tatgatgaaa
     3541 ttcaccgact acagcgtgaa aaccggacat ctgaccgcct actggacgcc atctttcgcc
     3601 caggacgtgt tggtgaaagc cagcgtcggg cagtatctgg caggggataa aggcggcacg
     3661 ctggagatcg ctaaacgctt tgacagcggc gtggtggtgg gtggctatgc cacgatcacc
     3721 aacgtttcga aagaggagta cggcgaaggg gacttcacca aaggcgtgta tgtctcggta
     3781 ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg cggcgattgg ctggacgccg
     3841 ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc agttgtatga catgaccagc
     3901 gaccgtagcg taaatttccg ctaagtcatg ggaaaggtgc cagttttcgc actcactggc
     3961 accctcattc ttaatgacct gttgagccat caggaacgca gttcaccccc ggtttacgat
     4021 ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt cgatatcggt tgcaggtctg
     4081 gttgcctgag cgactgggcc atcattgggc tggagacatt gctttgcggt aggtcggctt
     4141 tatcagcagt accgagcgga ccagcataag caggaagaac agagactgat aacatcaaag
     4201 cagcaaaata aggcttcatt tttaccacct ttatcaggtt acgtttcatt tgttccagag
     4261 gaacattgtc gatttttcgc gcattgctgg tggctgggaa tcacctgaat gggtgatttt
     4321 tgaattaccg gctttggtgc ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc
     4381 ggcctacggg taggtatatc cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa
     4441 aacatacaca aaaaatatag atctccgtca catttttgcg ttatacagga agctcgccac
     4501 tgtgaaggag gtactgctat gacgtcactc tctcgtccgc gcgtggagtt tatctccacc
     4561 attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg gcctgatttt ggttgtcttc
     4621 ctcggcaaag aaacggtgca tctggctgat gtgctgttcg cgccagaaca aaccagcaaa
     4681 tatgagctgg tagaaggact ggtggtttac tttctctatt tcgaatttat cgcgctgatt
     4741 gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt actttgtcta tattgggatc
     4801 accgccattg tgcgcttgat catcgtcgat cataaatcgc cactggatgt gttgatctac
     4861 tcggctgcga tcctgttact ggtgatcacc ctgtggctgt gtaattcgaa acggctgaag
     4921 cgggagtaaa aagacagcac gccgaaatgg cgcggcgtgc tggacaggaa gattacagcg
     4981 tagcagtttg ttgtgttttc ttcgtttccg gttcccagag cgcttccagc tcctcaaggg
     5041 ttttaccttt ggtttccggg acaaatttcc acataaacag tgctgccaga acgcccatac
     5101 aaccgtaaat ccagtaggag aaaccgttgt ggaaatgggc caccagccag gagtttttgt
     5161 ccatcatcgg gaaggtccag gagacgaagt agttcgccag ccactgggcc gccaccgcga
     5221 ttgccagcgc tttaccacga atagcattcg ggaagatttc cgacagcagt acccagcata
     5281 ccggacccca ggacatggca aaggcggcaa catagaacag catcgacagt agcgccacaa
     5341 tacccgatgc ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt gccattccga
     5401 gtgcgccgat aatttgcagt ggcttacgac caaatttatc cacagtcata attgccagta
     5461 cggtaaaggt gaggttgata actccgacaa taatggtctg caacagcgcg atatccgtgc
     5521 tggcccccag cgttttgaac acttccggcg cgtagtacag caccacattg atgccgacaa
     5581 attgctggaa gatggagagc ataacgccga ttacaatcac gcccacgcca aacatcagca
     5641 gacgaccacc ggttttgcgg ccatgatcca gggagtgttt aatttcctgt actgcctgag
     5701 ttgcaagcgt gttgcccata attttgcgca ggatactttc cgtctgttct tgcttgccgc
     5761 gtgacatcag ccagcgagga ctttctggca cggtatacag cagcattaag aacagcagtg
     5821 cagggataca ttccgaggca aacatataac gcctgccgtc agtattcagc cagctggcat
     5881 caccggaacg ggcaataaaa tagtttacgc agtaaactaa aagttgcccg aaaataatcg
     5941 caaactggtt aaatgaaacc agttttccgc gaatatgagc tggagccagt tccgcaatat
     6001 acattggtga aagcattgag gctaaaccaa cgccaatacc gccaataata cgataaataa
     6061 caaattccgg gacataacct gccagataaa taggtactgt gttgtccggg tttatagagg
     6121 taaaaccaag ttctggccag gcagaaccta caccagaaat aaaaaacagg acagcagaaa
     6181 tcttaagtga atcacgacgg ccgaagcggt tactgcaata accaccgagg gcaccgccga
     6241 tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa cagggagttg gcagcggatt
     6301 cacttaagtt ttgtggagca acaaagacgg tattgagtga ctcaacagta ccggaaataa
     6361 cggcggtgtc gtagccaaat aataaaccac ctaatgtagc gactaaggta atcgaaaata
     6421 tataactgga attatactgg gtattcattc agacctgcct tagaccattc tgatctttta
     6481 tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg
     6541 tttacgtaat gtcttttctg tgatcttaat tgtgataatt atcaaaaatt gatgaaacaa
     6601 tactattgcc gtgactcaga gcacgaaaga gaattatcgt aagtgggaaa acaaataacg
     6661 taaaaataat aagttctatg atgggaatat taaccggcga acgattcaga ttgcagacga
     6721 aagaaaaaaa ggcgctccgt ggagcgccga ataatagtca caagttggga taacgtaagt
     6781 tgagggtgca gcggcataac attggcagaa caacatcttt aacctttcac accacctgcc
     6841 gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga cgatggtgat cggtaatgca
     6901 gacatcacgg cagcggcggc aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc
     6961 tgcatcccca cggccagggt gtagctgttt acgtcacgca gtaacagcga cgcgaccgga
     7021 acttcagtaa tggcagcgat aaacgacagg atgaatacca ccgccagaat cggtactgac
     7081 agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg caccatccag cgcagccgct
     7141 tcttccagcg aactgtcgat ggtttcgaaa tagcctttga tggtccagac atgcagcgca
     7201 atcccaccca gatacgcgaa aattacgccg ccgtgagtat tcaggccaat gaatggaatg
     7261 tactcaccca gacgatcaaa caacgcatac aacgcgacca gtgaaagtac tgccgggaac
     7321 atctggaaaa tcagcattcc tttcagcagc gtcgctttgc ctggaaagcg catacgggcg
     7381 aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa tcgcggaaat cccagcgacc
     7441 tttaccgagt tccacagcca cagcagtacc gggaatggcg gtggcgtaat gcgaccatca
     7501 gcctgttcaa cgctaaaacc taacgccagt ttccagtgat cccaggagat ttgctccggg
     7561 atcaggctac cggtcgcgaa gtttccctga cgcagcgaga tagcgacgac catcagcagc
     7621 gggaacataa tcgccgcgat aaaaagtagc agtagcaggt gagtaataaa caaacgtgct
     7681 ttttgcgatt tcggttggac cattgccatt tttgttatct cccttaatca aacttcattc
     7741 gcgtggcttt caggttcact atcgccagcg cacccaccag caggaagatc agcgtggcaa
     7801 ttgctgccgc cagaccgaag tcctgacccc cgccgccttc aaaagcgata cggtaggtgt
     7861 agttaacaag caggtcggta taaccggctg gcgtggtcgt gccaagacga tccgggccgc
     7921 cgttggttaa cagctgaatc agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca
     7981 tcagcggcgt cagcggttta atcagcagcg gcagcgtaat cttaaagaag ttctggaacg
     8041 gacctgcgcc atccattgct gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc
     8101 ccatgcagag gatcatcatg tacggataac ccagccaggt attgacgata atcagcatcg
     8161 tgcgagcggt ggtcggatcg ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca
     8221 tcatgttgat ttcaccgaag ctctggttaa acaatccttt gaaaatcaag attgaaatga
     8281 acgatggcac cgcgtagggc agaatcagca ggacgcgata gaccgctttg ccgcgcaacg
     8341 cttcccactg caccagacat gccagaacca tgccgaccgc caccgttaaa aagacagtga
     8401 tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa cggtttctga atgccttcgt
     8461 cggtaaagac gcgggtaaag tttttccagc cggtggtcac ggtgtaaccg gggcttagct
     8521 tttcatcccc ccagttgccg tcggcggtaa tggactggta aaagccaatt tggttattcg
     8581 gacgatattt cacgccactc tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga
     8641 gcggctgcgt gccagaaaac tggcgcaggg agctcatcat cactttgttg ccatccggca
     8701 gaatggcggt aatgtcactc agcgcctgac gattctgggt aatcacgcgc agattcgcgc
     8761 gttcgccttc gggctgggcg gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt
     8821 taaacgcgtc ggagaggtaa tttttgccgg tttcgccgtc gctgagcgcc agttgccact
     8881 catcgcccgc cgggtaaaga ccaaagttat aggttttgcc tgcttgccag gagcgatcta
     8941 acaacacttc ctgcgcacgt tcaaaagtca gctggttagt gctgctgtag ttggtgaagg
     9001 caatggcgat ggtgcagacc agagggaaga ggacgaataa tcccattcca gccattcccg
     9061 ggtaaacata gcgccaggcg taggctttac gattggcgaa aatatacagc cccgctgaac
     9121 tcaatatcag cgtggtaatg gcgaacaggt attccccttg tgcgtacatt aaaacaacaa
     9181 ggtaacccac cagcaggccg agcagaccta gcactgacca tttcagcgcg tcgctttgcc
     9241 accaatgttt ctttttaatg acatccatgg ggttcttcct cattccagga cggataaggc
     9301 tttcacgcct tatccgacaa caactgcctg atgcgacact gatgcgtctt atcaggccta
     9361 catgcgtttc agttttgtag gccggacaag gcgttcacgc cgcatccggc atttcacagc
     9421 attacttggt gatacgagtc tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc
     9481 tggcggcgtt gatcaccgca gtacgtacgg cataccagaa agcggacatc tgcgggatgt
     9541 tcggcatgat ttcacctttc tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg
     9601 ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct ttattaaccg
     9661 cttccagacc ttcatcagtc agcagatagt tttcgaggaa ctctttcgcc agctctttgt
     9721 tcggactggc ggcgttaata ccggtgctca gcacgccaac gaacggttta gatggttgac
     9781 ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg atgttggacc
     9841 atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca gcttctgcga
     9901 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca
     9961 gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt
    10021 catacttgaa cgcataaccc ccgtcagcag caatcagcgg ccaggtgaag tacggttctt
    10081 gcaggttgaa catcagcgcg ctcttacctt tcgctttcag ttctttatcc agcgccggga
    10141 tctcttccca ggtttttggc gggttcggca gcaggtcttt gttataaatc agcgataacg
    10201 cttcaacagc gattgggtaa gcaatcagct tgccgttgta acgtacggca tcccatgtaa
    10261 acggatacag cttgtcctgg aacgctttgt ccggggtgat ttcagccaac aggccagatt
    10321 gagcgtagcc accaaagcgg tcgtgcgccc agaagataat gtcagggcca tcgccagttg
    10381 ccgcaacctg agggaatttc tcttccagtt tatccggatg ctcaacggtg actttaattc
    10441 cggtatcttt ctcgaatttc ttaccgactt cagcgagacc gttatagcct ttatcgccgt
    10501 taatccagat taccagttta ccttcttcga ttttggcgag agccgaggcg gaaaacatca
    10561 tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt ttttattttc ataatctatg
    10621 gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga ctctagtttc tttatacggc
    10681 aacctctttc catcctcctt gcccctacgc cccaccgttg ctttgtgtga tctctgttac
    10741 agaattggcg gtaatgtggg gatgcgcaca taaaatcgcc acgatttttg caagcaacat
    10801 cacgaaattc cttacatgac ctcggtttag ttcacagaag ccgcgttctc atcctcccgc
    10861 ctcctccccc ataaaaaagc cagggggtgg aggatttaag ccatctcctg atgacgcata
    10921 gtcagcccat catgaatgtt gctgtcgatg acaggttgtt acaaagggag aagggcatgg
    10981 cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga ggtcgtggta tcgaaagata
    11041 tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta
    11101 aatcgacttt actgcgcatg attgccgggc ttgagacgat caccagcggc gacctgttca
    11161 tcggtgagaa acggatgaat gacactccgc cagcagaacg cggcgttggt atggtgtttc
    11221 agtcttacgc gctctatccc cacctgtcag tagcagaaaa catgtcattt ggcctgaaac
    11281 tggctggcgc aaaaaaagag gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctgc
    11341 aactggcgca tttgctggat cgcaaaccga aagcgctctc cggtggtcag cgtcagcgcg
    11401 tggcgattgg ccgtacgctg gtggccgagc caagcgtatt tttgctcgat gaaccgctct
    11461 ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac
    11521 gcctgggccg cacaatgatt tacgtcaccc acgatcaggt cgaagcgatg acgctggccg
    11581 acaaaatcgt ggtgctggac gccggtcgcg tggcacaggt tgggaaaccg ctggaactgt
    11641 accactatcc ggcagaccgt tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc
    11701 tgccggtaaa agtgaccgcc accgcaatcg atcaagtgca ggtggagctg ccgatgccaa
    11761 atcgtcaaca agtctggctg cctgttgaaa gccgtgatgt ccaggttgga gccaatatgt
    11821 cgctgggtat tcgcccggaa catctactgc cgagtgatat cgctgacgtc atccttgagg
    11881 gtgaagttca ggtcgtcgag caactcggca acgaaaccca aatctatatc cagatccctt
    11941 ccattcgtca aaacctggtg taccgccaga acgacgtggt gttggtagaa gaaggtgcca
    12001 cattcgctat cggcctgccg ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat
    12061 gtcgtcgact gcataaggag ccgggcgttt aagcacccca caaaacacac aaagcctgtc
    12121 acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat agaatgatga ttactctgcg
    12181 caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt
    12241 tgatttccac ggctatgcac gttccggtat tggctggaca ggtagcggcg gtgaacaaca
    12301 gtgtttccag actaccggtg ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta
    12361 tgctgaatta aaattgggtc aggaagtgtg gaaagagggc gataagagct tctatttcga
    12421 cactaacgtg gcctattccg tcgcgcaaca gaatgactgg gaagctaccg atccggcctt
    12481 ccgtgaagca aacgtgcagg gaaaaaacct gatcgaatgg ctgccaggtt ccaccatctg
    12541 ggcaggtaag cgcttctacc aacgtcatga cgttcatatg atcgacttct actactggga
    12601 tatttctggt cctggtgccg gtctggaaaa catcgatgtt ggcttcggta aactctctct
    12661 ggcagcaacc cgctcctctg aagctggtgg ttcttcctct tttgccagca acaatattta
    12721 tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat
    12781 caacccgggc ggcacattag aactgggtgt cgactacggt cgtgccaacc tgcgtgataa
    12841 ctatcgtctg gttgatggcg catcgaaaga cggctggtta ttcactgctg aacatactca
    12901 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
    12961 gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt gataacgaaa aatttgccta
    13021 caatatcaac aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
    13081 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
    13141 cggcaccaag tggtggaccg ttggtattcg cccgatgtac aagtggacgc caatcatgag
    13201 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
    13261 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
    13321 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt acgactacaa
    13381 cggcgatagc aaggttaacc cgaactacgg caaagccgtt cctgctgatt tcaacggcgg
    13441 cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gcccagatgg aaatctggtg
    13501 gtaatagcaa aacctgggcc ggataaggcg tttacgccgc atccggcata aaaacaggtt
    13561 gtcattatct gaaaggggcg aaagcccctc tgattatcgg gtttagcgcg ctattgcctg
    13621 gctaccgctg agctccagat tttgaggtga aaacaatgaa aatgaataaa agtctcatcg
    13681 ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg aattagcctt gccgatgtta
    13741 actacgtacc gcaaaacacc agcgacgcgc cagccattcc atctgccgcg ctgcaacaac
    13801 tcacctggac accggtcgat caatctaaaa cccagaccac ccaactggcg accggcggcc
    13861 aacaactgaa cgttcccggc atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca
    13921 ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc
    13981 cgaacgtgct gattcttgat cagaacatga ccccatcagc cttcttcccc agcagttatt
    14041 tcacctacca ggaactaggc gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga
    14101 caccggcgtt ggggcagcaa aaactttatg ttctggtctt taccacggaa aaagatctcc
    14161 agcagacgac ccaactgctc gacccggcta aagcctatgc caaaggcgtc ggtaactcga
    14221 tcccggatat tcccgatccg gttgctcgtc ataccagcga tggcttactg aaactgaaag
    14281 tcaaaacgaa ctccagctcc agcgtgttgg taggaccgct atttggttct tccgctccag
    14341 ctccggttac ggtaggcaac acggcggcac cagctgtggc tgcacctgct ccggtgccgg
    14401 tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa
    14461 acgctgtcgc gaaaggtgat gttgataagg cgttaaaact gcttgatgaa gctgaacgcc
    14521 tgggatcgac atctgcccgt tccaccttta tcagcagtgt aaaaggcaag gggtaattac
    14581 gccccacagt gctgattttg caacaactgg tgcgtctcct ggcgcacctt tttttatgct
    14641 tccttcctgg gatatgagcg attttttata gtacttcact tcttcttcac taagaatgtc
    14701 cattatttca atgcattatc agagattctt ttcctttcgc cggtagtgtc tggacgctca
    14761 ggctactttt ccaggttatt ttatttctgt attacagagg ttttatgata ggtcatatca
    14821 caaattctgg cggcaataac tctttgatga aacatgatgt ggtgcaagga aataatatag
    14881 tagatcttga tttactacgt aatttcaatg gggtgacagg tttaaacaga gattacttta
    14941 tttatatc
//
LOCUS       DAZPFP010000088        14704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPFP010000088 DAZPFP010000000
VERSION     DAZPFP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.088"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(8..1270)
                     /locus_tag="ACHUR4_002502"
     CDS             complement(8..1270)
                     /locus_tag="ACHUR4_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1456055.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1468..1543)
                     /locus_tag="ACHUR4_002503"
     tRNA            complement(1468..1543)
                     /locus_tag="ACHUR4_002503"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1508..1510),aa:Phe,seq:gaa)
     gene            complement(1649..2356)
                     /locus_tag="ACHUR4_002504"
     CDS             complement(1649..2356)
                     /locus_tag="ACHUR4_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1456056.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2509..2592
                     /gene="yqgH"
                     /locus_tag="ACHUR4_002505"
     CDS             2509..2592
                     /gene="yqgH"
                     /locus_tag="ACHUR4_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1456057.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2754..4889
                     /gene="speC"
                     /locus_tag="ACHUR4_002506"
     CDS             2754..4889
                     /gene="speC"
                     /locus_tag="ACHUR4_002506"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1456058.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4938..6194)
                     /gene="nupG"
                     /locus_tag="ACHUR4_002507"
     CDS             complement(4938..6194)
                     /gene="nupG"
                     /locus_tag="ACHUR4_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1456059.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6396..7475)
                     /gene="mltC"
                     /locus_tag="ACHUR4_002508"
     CDS             complement(6396..7475)
                     /gene="mltC"
                     /locus_tag="ACHUR4_002508"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1456060.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7540..7815)
                     /locus_tag="ACHUR4_002509"
     CDS             complement(7540..7815)
                     /locus_tag="ACHUR4_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1456061.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7843..8895)
                     /gene="mutY"
                     /locus_tag="ACHUR4_002510"
     CDS             complement(7843..8895)
                     /gene="mutY"
                     /locus_tag="ACHUR4_002510"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1456062.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9056..9775
                     /gene="trmB"
                     /locus_tag="ACHUR4_002511"
     CDS             9056..9775
                     /gene="trmB"
                     /locus_tag="ACHUR4_002511"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1456063.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9775..10101
                     /locus_tag="ACHUR4_002512"
     CDS             9775..10101
                     /locus_tag="ACHUR4_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1456064.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10285..11004
                     /locus_tag="ACHUR4_002513"
     CDS             10285..11004
                     /locus_tag="ACHUR4_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1456065.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11180..12226
                     /gene="ansB"
                     /locus_tag="ACHUR4_002514"
     CDS             11180..12226
                     /gene="ansB"
                     /locus_tag="ACHUR4_002514"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1456066.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12343..13305
                     /locus_tag="ACHUR4_002515"
     CDS             12343..13305
                     /locus_tag="ACHUR4_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1456067.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13406..>14704)
                     /locus_tag="ACHUR4_002516"
     CDS             complement(13406..>14704)
                     /locus_tag="ACHUR4_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR1456068.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3835 a         3550 c         3790 g         3529 t
ORIGIN      
        1 ctgcttacta ttttaatgga ttgttaatct ttgcatattc aaacgggctg atacccttct
       61 cccggttcgc atctagaaaa tcggcccacc attgcaacat cagtcgccgt tcttccagat
      121 gttctgcttt atggatatac gcagcacgta caccattacg ctcctgatgg ctcatctgac
      181 gttccacagc atcgcgggac cacaaacctg attcaatcaa tgcactgcac gccatcgccc
      241 ggaatccatg gccacagact tcctgggttg tgtcataccc catcaccctc agggctttat
      301 ttacagtatt ttcactgatt ggtttacgcg ggtcatgtgc acccgtaaag atcagaccat
      361 tttcaccagc ccatgtctga agttctgcca gtatggctac aacctgtttg ctgagtggca
      421 caagatgtgg tgttcgcatc ttggatcccc ggtgtgagaa ttttacgcct ggcagaggtt
      481 cgcgttcggc tggaatagtc cacatggaat tgtcaatatc aatttccgac cagcgggcat
      541 atctgagttc actggaacga ataaaaacca gtaaattcag cataactgcc agtcgggtga
      601 caggctggcc tttataaccg tcaatacgcg acagaagttc aggcaggcgt gaaagctcca
      661 gagcagggcg atgctggcgt tttaccgtgg tcaatgcacc agccatatcc agagccgggt
      721 tatatctgat gatgccagac tgtacggcgt aacgcattac agcagatatt ctttgctgca
      781 gacgactggc gatttcataa atttgttttg cttctgcagc acgaacaggg ataagcaggt
      841 ccggggtatt gagtgttgtg atatcccggt tgccgataaa ggggaaaacg tgtgtctcga
      901 ggcttttgag tactttatcg ctgtgtgatt gtgcccattg tttattactg gcatgccagc
      961 gtcgtgcaat atgttcaaaa gagagctgta aatcagggac aactttttta actcttttct
     1021 gttcgcaagg gtcaatcccc tctgaaatca gttttcgggc ctcatcccgt tttgtacgag
     1081 cttcggagag agaaacttct ggatacacac caagtgccag agtcttctcc ttacccagaa
     1141 tacgataacg gagacgccag taacgggaac cattaggatg aaccagcagg aacatgccgg
     1201 ccccgtcagt gagtttataa gccttgtcag tgggctttgc agcccggatt tttgcgtcag
     1261 tcagtgccat agttcagcct tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg
     1321 ggtatacccg caattatacc cacacaatgg ggttgatgtg gggggaatct ggttgagttc
     1381 ggtagaattg atttggagga agaacactta aatcatggtg ttctgaaacg aaaaacggac
     1441 ctccgtggag gtccgttgat atgaatttgg tgcccggact cggaatcgaa ccaaggacac
     1501 ggggattttc aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac
     1561 ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat
     1621 catcactttg ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa
     1681 gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac
     1741 agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca
     1801 tcgacggtgt gatcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga
     1861 tcagtaatgg gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg
     1921 ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt
     1981 cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat
     2041 aattctgaat aaaagattca tgcgctggct tcttacgtga gtgacgaaac agattttgtg
     2101 ctttggcgac tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc
     2161 caagtagcgt agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac
     2221 caatccccag cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta
     2281 agcgttggct gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa
     2341 aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac
     2401 tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc cttagcgaaa
     2461 ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccatc
     2521 tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg
     2581 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     2641 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc
     2701 agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat
     2761 caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat cgccgcgtgg
     2821 tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata
     2881 gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt
     2941 attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc
     3001 agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt
     3061 tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac
     3121 atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg ccatttttac gatttctttg
     3181 gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta
     3241 ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata
     3301 aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt
     3361 taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg
     3421 cgctgattca ggcggggacg acgccggtct atctggaagc ttcacgtaac ccgtttggtt
     3481 tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg
     3541 acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg cctgacgatt attcagctgg
     3601 gaacctatga cggcactgtc tataacgccc gtcaggtgat cgataccatt gggcatctgt
     3661 gtgattacat tctgtttgat tccgcgtggg tcggttacga acagtttatc tcgatgatgg
     3721 cggatagctc gccgctgctg ttagaactta acgaaaacga tccggggatt tttgtgaccc
     3781 agttggtgca caaacagcag gctggattct cacagacgtc gcagatccat aaaaaagata
     3841 accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataac gcctttatgc
     3901 tccatgcttc taccagccct ttctatccgc tgtttgctgc actggatgtt aacgccaaaa
     3961 ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt
     4021 cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg
     4081 atggcaaatt gtggcaggat tatccgacat cagtgttagc cagcgaccgc cgttttttca
     4141 gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg
     4201 ttgatccgtg caagctgtta ctcactacac caggtatcaa tgccgaaacc ggcgaatata
     4261 gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc
     4321 cggagaagtg cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga
     4381 agctggcaca actggtggcg atgctggcgc aatttgaaca gcatattgag gatgactcgt
     4441 cgctggctga ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca
     4501 ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc
     4561 tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg
     4621 cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc
     4681 gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg
     4741 aagtctgggg tggggcggtt caacgttatt tccttgcgct ggaagaaggc gtgaatttgt
     4801 tgccgggatt ttcgccggag ttgcaaggtg tctatagcga aaccgatgcg gatggcatga
     4861 aacgattgta cggttatgtg ttgaagtaag aataaaaaaa cgggtcacct tctggcgacc
     4921 cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc ctgtcgggac acgaacgtgt
     4981 ttatatttga acatcgccat gaacgcgaag gccagaacca cggaataacc agcgaaaatc
     5041 aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt acatctcaac aactttaccg
     5101 ctcacgatgc cgccgaggat acagccgaag ccgttagtca tcatcaggaa catcccttgt
     5161 gcactggcgc gaattgccgg gctaacttct ttttcgacaa acaccgaacc agagatgttg
     5221 aagaagtcga atgcgcaacc gtaaacgatc atcgacagta ccagcagtac agtaccgaac
     5281 ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga tccacgccac aatactgatc
     5341 atcattacgt tcttaatacc gtagcggctt aagaagaacg ggatggtcag aatgaacagg
     5401 gtttcagaga tctgcgaaat cgacatgatg attgacgcat gctgcacgat gaagctgctg
     5461 gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat taccgaacat gttggtaatc
     5521 tgcagttccg cgcccagcag cattgagaag atgaagaaga ttgccatacg cttgttttta
     5581 aacagcgcga atgcatcgag gcccagcagg gttgtccagc tctgattcgc ttgctgttta
     5641 gcaaccggaa tatgcggcag agtcagggta aacagaacca gaatggcgga aagtgctgcg
     5701 ccaatataca gctgcatgtg gcttaattcg aagccagaca ggctcaccac ccacattgcc
     5761 atgataaagc cgatggtgcc ccagatacgg attggcggga agtcagtaac gatatccatc
     5821 ccggcatttt gcaggcgata gtaagagatg gtgttgatta acccaagcgt tggcatataa
     5881 gcaaacgagt taatcaatat cacaaggaac atcgcttccg gcgtcgtgac ctgtgccgcc
     5941 atgaacagcg tgatagcgcc aatggtgtgg caaatggcat atacccattt cgcacttaac
     6001 catttgtcgg ccacaatccc cagcagcgca ggcataaata ccgcggcgat acccagtgag
     6061 ctataaactg cgccaataga agcaccgtca aacttcaggg taacaaacat ataggagccg
     6121 agggtcgtca gccaacttcc ccacagacag aactgcagaa aagagaggat tttcagctgc
     6181 agcttaagat tcatgttaat ttcctcacat cgtgatgcgg atgagtgttt gaaaaagcac
     6241 atttgaccgt gcgagatgat gggattctat caacttcgcg gactggtttt ttgttacctg
     6301 tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa ttgtgatgtg gataacattt
     6361 ttgcccctga gcatcgtcag gggcggttaa tggaattatc ggcggcggta ggatttttgc
     6421 gcggtattca ctttataaag ataacggcga gattccgcag agggatggcg ggtcgtcagc
     6481 gtctgataaa catcgcccgg cgtcatggtg ttaataatat tggcagcctg aatcttatca
     6541 ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat aggcggtgat gacggcataa
     6601 cgccgcgatg ttgggttatc aattccgccg agataaacat tgttcagcat cgccagatac
     6661 gcggtgccgg tatcaatatt gctggcagga tcaaacaaga aactgcggct cggcgtgccg
     6721 gatttcccct gcgagcggaa cacatctttc ccggcagtat tttgtaccac ctgcattaat
     6781 cccagcgcat cggaacggct gaccgcatac gggttaaagg aagattcagt ctgcataatt
     6841 gccagaatca gcgactcatc aacgccatat ttccgtgacg cctggcggac catgccgaga
     6901 tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt taatggtgac gctgtagatg
     6961 atacgtagcc cgttgctgcg gcttttcaga cggtttttca gcagataatc cgcgaagttg
     7021 cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt ccaccacctg accgtaaagg
     7081 aaaggttctt tcgaaattgt aatatcatca acgtcggaat agagatcgac cgaactcgga
     7141 tcgtcaccca tcagcaacgt tttgataatt gcccggcgca aatgcgcggc aggttctgtc
     7201 ccggcgatgg tttcgatagt aatcgtaccg tcatcgaagt tgatgtggct gcgggtctga
     7261 tattgatcgg tgtatttcac gtagtcctta ggaccggcga tcaccacctc tttgaagccc
     7321 cagatgttct caatattgtg ggcaaattgc cccatcagaa tatcaaaacc gttggtatct
     7381 ttgacccagg cttcgttata ggtatcgcct tttttggtcg tcgaacagga gatgagcaac
     7441 ggcgcaatca aagccagcgc gagatatttt ttcatcattc cgggtgcgtg ttgtgtttgt
     7501 ttttatgcaa gctgccggag gcgtgctccg gcactgtttt tattttttat cttccggcgt
     7561 atagccctcg atatgcacct ctttaccctc gaacaggaag ttgaccatct cctgctccag
     7621 cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt tcattaatca gcatggtttg
     7681 tttgtgctgc cactgcgccc aggcttcttt ggagatctcg ttatagatgc gttttcccag
     7741 ctcgccgggg tacagctgaa aatcctgacc ttctgcttca cgttgcagga aagtacaaaa
     7801 aatcgttctg ctcataaatc atcctcttta tcgactcacg cgctaaaccg gcgcgccagt
     7861 gcgtaactgc tgtaacaaac gctccacggg agccgccagg ccaactgacg gcggttgcgc
     7921 taagttatac cagagcgcat tgccttcatc catgcagccg gtgaatgacg acacgggaag
     7981 ccacataggc acaatatcta agtggaaatg gctgaaggta tggcgaaacg cggtcagttg
     8041 cgtcaggtta tcggcagcaa tctgccgttg tgccagccaa tgccgcaaac tttcttcgtc
     8101 ggcaaactgc gggaaacagt ataaaccgcc ccacaatccg ctcggcggac gctgcgccag
     8161 caatacttca tcttcgtgct gtaacaggag aaagtagccg gtgcgctccg gcagcgtctg
     8221 tttcggtttt ttgcccggat aaagcgacca gctattgttg gtggcggcaa tacatccgtt
     8281 ttgtagcgga cagagcgaac atttcggctt cgaacgcgtg caaatcatcg cgcccaaatc
     8341 catcatcgcc tgattaaacc gttccacgcc gaccgcgggc gtcacctgct cgctcaaact
     8401 ccatagttta ttctcgacct cttttttccc aggccagccg cttacagcat agcagcgcgc
     8461 cagcacgcgt ttgacgttac cgtcgagaat cggaaagtgc ttacccagag aaagcgagag
     8521 aatcgcgcct gcggtggaac gcccgacgcc cggtaacgcc gcgacttctt caaaggtttc
     8581 cgggaattta ccaccgtgta aggtcgccac ttgttgtgcc gctttatgca ggttgcgcgc
     8641 gcgggcgtaa tagccaagcc cggtccacaa gtggagaact tcgtcgagcg gcgcattggc
     8701 gagatcggtc accgtcggga agtgcgccat aaagcgttca aaatagggga taacggtcgc
     8761 aacctgagtt tgttgcaaca tcacttctga gagccatact ttgtagggcg tcttgtcaat
     8821 ttgccagggc agcgttttcc gcccgtattt atcgtaccag tccagaacct gggctgaaaa
     8881 ttgcgacgct tgcatggtca ccgacttcac tgttgttggg ggcaagattg cagcacagcg
     8941 acggcagggt gtaaaccgga actttccgca gccacggcct tatcatgctt gcatccggca
     9001 actaactttg gataatgccc gttttcagaa cactttcaca agcgactaaa tctttatgaa
     9061 aaacgacgtc atttcaccgg aatttgatga aaacggccgc ccactgcgcc gtatccgtag
     9121 ttttgtgcgc cgccaggggc gactgaccaa aggccaggaa catgcgctgg aaaactactg
     9181 gccggtgatg ggcgttgagt tcagtgaaga tatgctggat ttccccgcgc tttttggccg
     9241 tgaagcgccg gtgacgcttg agattggttt tggcatgggg gcgtcgctgg tggcaatggc
     9301 taaagatcgc cccgagcagg acttcctcgg cattgaagtg cattcaccgg gcgttggtgc
     9361 gtgcctggct tctgcgcatg aagaaggttt aagcaacctg cgcgtgatgt gtcacgatgc
     9421 ggttgaagtg ctgcataaaa tgattcctga caattcattg cgcatggtgc agctcttttt
     9481 ccctgacccg tggcacaaag cgcgccataa taaacgccgt atcgttcagg tgccgtttgc
     9541 cgaactggta aaaagcaaac tgcagctggg gggcgtattc catatggcga ccgactggga
     9601 accttatgcg gaacatatgc ttgaagtgat gtcttctatt gacggttata aaaacctgtc
     9661 agagagcaat gattacgtac cgcgtccggc atcacgtccg gtgacgaaat ttgaacaacg
     9721 tggtcatcgt cttggtcacg gagtatggga cttaatgttc gagagggtga aataatggca
     9781 aagaaccgta gccgtcgtct gcgtaaaaaa atgcacatcg acgaattcca ggaattagga
     9841 ttttcggtgg catggcgatt cccggaaggt acatcggaag aacagattga taaaatcgtt
     9901 gatgacttta ttaacgaggt tatcgaaccg aacaaactgg cctttgacgg cagcggttat
     9961 ctggcctggg aaggtctgat ctgcatgcag gaaatcggca aatgcaccga agaacatcag
    10021 gcgattgtgc gtaagtggct ggaagagtgc aaactggatg aggtacgcac cagcgaactt
    10081 ttcgacgttt ggtgggacta agaaagcata cgggcgatga caaatgcaaa actgcctgat
    10141 gcgctacgct tatcaggcct acagagatgc acgatcgagt aggcgggata aggcgtttac
    10201 gccgcatccg gcatggaaaa cgcgtacttt gttatcaatc tggggccagc atatgctggc
    10261 ctgatttgtt cttgagggaa gactatgatg cgcaaaatgc tgctggcggc agcactttca
    10321 gtgacggcaa tgaccgctca cgccgactac cagtgcagcg tcacgccgcg tgacgatgtg
    10381 attgtcagcc cgcaaaccgt gcaggtgaag ggcgaaaacg gcaatctggt gatcacgcca
    10441 gacggcaacg tgatgtataa cggtaagcaa tattccctga atgccgccca gcgcgagcag
    10501 gcgaaggatt atcaggctga actacgtagc acgctgccgt ggattgatga aggcgcgaaa
    10561 agccgcgtcg agaaagcccg tattgcgctg gataaaatta tcgttcagga gatgggcgac
    10621 agcagcaaaa tgcgcagccg tctgaccaaa cttgatgcgc agcttaaaga gcagatgaat
    10681 cgcattatcg aaacgcgcag cgatggcctg acgttccact ataaagccat tgatcaggtt
    10741 cgcgccgaag gccagcaact agtgaatcag gcaatgggcg gaattttaca ggacagcatt
    10801 aatgaaatgg gcgcgaaagc ggtgctgaaa agcggcggta acccattaca gaatgtgctg
    10861 ggaagcttgg gcggtctgca atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat
    10921 ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa
    10981 gccctggtcg ggaatttaaa ataatcctct attttaagac ggcataatac ttttttatgc
    11041 cgtttaattc ttcgttttgt tacctgcctc taactttgta gatctccaaa atatattcac
    11101 gttgtaaatt gtttaacgtc aaatttccca tgcaaagcta agggataatg cgtagcgtcc
    11161 acgtaactgg aggaatgaaa tggagttttt caaaaagacg gcacttgccg cactggttat
    11221 gggttttagt ggtgcagcat tggcattacc caatatcacc attttagcaa ccggcgggac
    11281 cattgccggt ggtggtgact ccgcaaccaa atctaactac acagcgggta aagttggcgt
    11341 agaaaatctg gttaatgcgg tgccgcaact aaaagacatt gcgaacgtta aaggcgagca
    11401 ggtagtgaat atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa
    11461 aattaacgcc gactgcgata aaaccgacgg cttcgtcatt acccacggta ccgacacgat
    11521 ggaagaaact gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt
    11581 cggcgcaatg cgcccgtcca cgtccatgag cgcagacggt ccattcaacc tgtataacgc
    11641 ggtagtgacc gcagctgata aagcctccgc taatcgtggc gtgctggtgg tgatgaacga
    11701 caccgtactg gacggtcgcg atgtcaccaa aaccaacacc accgacgtag cgaccttcaa
    11761 gtctgttaac tacggtcctc tgggatacat tcacaacggt aagattgatt accaacgtac
    11821 cccggcacgt aagcacacca gcgatacgcc attcgatgtc tctaagctga atgagctgcc
    11881 gaaagtcggc atcgtttata actacgctaa cgcatccgat cttccggcta aagcactggt
    11941 agatgcgggc tatgatggca tcgttagcgc tggtgtgggt aatggtaacc tgtataaatc
    12001 cgtgttcgac accctggcaa ccgccgcgaa aaacggcact gcagtagtgc gttcttcccg
    12061 cgtaccgacg ggtgctacca ctcaggatgc tgaagtggat gatgcgaaat acggcttcgt
    12121 cgcctctggc acgctgaacc cgcaaaaagc gcgcgtcctg ctgcagctgg ctctgacgca
    12181 aaccaaagat ccgcagcaga tccagcagat tttcaatcag tactaatcac ctcgtcccgg
    12241 catcgtgccg gggctttttc acttcagact tacgtccatt gccaattttc attaccctaa
    12301 tgataatcac cggaataaat tattccgcgc gagggttttc gggtgaaaaa gcaatggatt
    12361 gttggcacgg cgctgcttat gttgatgact ggtaatgcct gggcagatgg cgaaccgcca
    12421 actgaaaata tcttaaaaga tcaattcaaa aagcagtatc acggcattct caagcttgat
    12481 gccatcagct taaaaaatct tgatgctaag ggtaatcagg ccacctggtc agcggaaggc
    12541 gatgtctctt ccagtgacga tctctatacc tgggtcggtc agttggcaga ttacgaactg
    12601 ctcgaacaga cctggacgaa agataaaccg gtaaaattct cggcgatgtt aaccagtaaa
    12661 ggaacgcccg cgtctggctg gtcggtgaac ttttactctt ttcaggcggc agccagcgat
    12721 cgtgggcggg tggtcgacga tatcaaaacg aataataaat atctgatcgt gaatagcgaa
    12781 gatttcaatt atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt
    12841 cctgcgctgg aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca
    12901 gcggatgcat attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt
    12961 aaaaaaattc accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg
    13021 gttaaatatg acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa
    13081 gagtgttatg aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga
    13141 cagacttttc tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc
    13201 gaaaaagggc aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg
    13261 attttgatga aaattgacga tatcaaccaa gctaatgaac gctagaaaaa agataccgaa
    13321 caactgcggc gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat
    13381 aaaaagttat ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg
    13441 atatttgcgg aatgtaagtg atcatcatca acgctactag caatgcagca taaaatagca
    13501 gcaatggttt gattaaatgt tgaatcttca cacccgagat agaacaacca acaaatagcg
    13561 cactacccac cggcggtgtt aacagcccaa tacaaaggtt ggccaccatc ataataccga
    13621 aatgtactgg gtccattccc agctcttgtg caatcggcaa gaagatcggt gtaaagatta
    13681 gtacagccgg tgtcatgtcc ataaagatgc cgacaattaa taagacgata ttaatcagca
    13741 gcaatatgat cagtgggtta tcagaaatcc ccatcaatgc gtcgctgatc atataaggaa
    13801 tatcagcatt agtcattgcc caggacatgc ccacggaaaa accgatcaac agtaatacaa
    13861 tcgaagtggt aacaaccgac tccagaatca gtttgggtaa atggcgccat tttacttccc
    13921 ggtaaatcag caccgagaga ataaatgtgt agacaacggc aattgctgac gcttcagttg
    13981 cggtaaaaat gccgcccaga atgccgccca tcacaatgaa caccagaagc aaacttggta
    14041 aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa agcgggtagc
    14101 cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt aagatccctg
    14161 gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc agggaaaaca
    14221 caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag acgtttacag
    14281 cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt gtgccaccaa
    14341 cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata ttcgccagga
    14401 cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg aggttaatca
    14461 aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa aaggggattg
    14521 ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg attgccgcga
    14581 tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt gagaacgaaa
    14641 tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc gcgatataac
    14701 tatc
//
LOCUS       DAZPFP010000089        14601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPFP010000089 DAZPFP010000000
VERSION     DAZPFP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.089"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUR4_002517"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUR4_002517"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1456069.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUR4_002518"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUR4_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1456070.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUR4_002519"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUR4_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1456071.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUR4_002520"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUR4_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1456072.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHUR4_002521"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHUR4_002521"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1456073.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACHUR4_002522"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACHUR4_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1456074.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACHUR4_002523"
     CDS             5920..6417
                     /locus_tag="ACHUR4_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1456075.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACHUR4_002524"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACHUR4_002524"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1456076.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUR4_002525"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUR4_002525"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1456077.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUR4_002526"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUR4_002526"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1456078.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACHUR4_002527"
     CDS             9102..9665
                     /locus_tag="ACHUR4_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1456079.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUR4_002528"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUR4_002528"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1456080.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACHUR4_002529"
     CDS             complement(10257..11237)
                     /locus_tag="ACHUR4_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1456081.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUR4_002530"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUR4_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1456082.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUR4_002531"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUR4_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1456083.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUR4_002532"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUR4_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1456084.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACHUR4_002533"
     CDS             12838..13431
                     /locus_tag="ACHUR4_002533"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1456085.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUR4_002534"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUR4_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1456086.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3519 a         3862 c         3752 g         3468 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat c
//
LOCUS       DAZPFP010000090        14481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPFP010000090 DAZPFP010000000
VERSION     DAZPFP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.090"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUR4_002535"
     CDS             <1..118
                     /locus_tag="ACHUR4_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456087.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHUR4_002536"
     CDS             complement(115..381)
                     /locus_tag="ACHUR4_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1456088.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUR4_002537"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUR4_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1456089.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUR4_002538"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUR4_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUR4_002539"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUR4_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1456090.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUR4_002540"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUR4_002540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1456091.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUR4_002541"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUR4_002541"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1456092.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUR4_002542"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUR4_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1456093.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUR4_002543"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUR4_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1456094.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUR4_002544"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUR4_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1456095.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUR4_002545"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUR4_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUR4_002546"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUR4_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHUR4_002547"
     CDS             complement(12643..14097)
                     /locus_tag="ACHUR4_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR1456096.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHUR4_002548"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHUR4_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1456097.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZPFP010000091        14274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPFP010000091 DAZPFP010000000
VERSION     DAZPFP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.091"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            35..601
                     /locus_tag="ACHUR4_002549"
     CDS             35..601
                     /locus_tag="ACHUR4_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1456098.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            652..3111
                     /locus_tag="ACHUR4_002550"
     CDS             652..3111
                     /locus_tag="ACHUR4_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1456099.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3126..3857
                     /locus_tag="ACHUR4_002551"
     CDS             3126..3857
                     /locus_tag="ACHUR4_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1456100.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3854..4930
                     /locus_tag="ACHUR4_002552"
                     /pseudo
     CDS             3854..4930
                     /locus_tag="ACHUR4_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5082..6128
                     /locus_tag="ACHUR4_002553"
     CDS             5082..6128
                     /locus_tag="ACHUR4_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1456101.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6125..6916)
                     /gene="nei"
                     /locus_tag="ACHUR4_002554"
     CDS             complement(6125..6916)
                     /gene="nei"
                     /locus_tag="ACHUR4_002554"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1456102.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6952..7686)
                     /gene="pxpA"
                     /locus_tag="ACHUR4_002555"
     CDS             complement(6952..7686)
                     /gene="pxpA"
                     /locus_tag="ACHUR4_002555"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1456103.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7676..8608)
                     /gene="pxpC"
                     /locus_tag="ACHUR4_002556"
                     /pseudo
     CDS             complement(7676..8608)
                     /gene="pxpC"
                     /locus_tag="ACHUR4_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8602..9258)
                     /gene="pxpB"
                     /locus_tag="ACHUR4_002557"
     CDS             complement(8602..9258)
                     /gene="pxpB"
                     /locus_tag="ACHUR4_002557"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1456104.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9281..10024)
                     /gene="ybgI"
                     /locus_tag="ACHUR4_002558"
     CDS             complement(9281..10024)
                     /gene="ybgI"
                     /locus_tag="ACHUR4_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1456105.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10295..11776
                     /gene="dtpD"
                     /locus_tag="ACHUR4_002559"
     CDS             10295..11776
                     /gene="dtpD"
                     /locus_tag="ACHUR4_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1456106.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11926..13344)
                     /gene="phrB"
                     /locus_tag="ACHUR4_002560"
     CDS             complement(11926..13344)
                     /gene="phrB"
                     /locus_tag="ACHUR4_002560"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1456107.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13341..13850)
                     /locus_tag="ACHUR4_002561"
     CDS             complement(13341..13850)
                     /locus_tag="ACHUR4_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1456108.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            13979..14266
                     /locus_tag="ACHUR4_002562"
     CDS             13979..14266
                     /locus_tag="ACHUR4_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR1456109.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         3461 a         3831 c         3496 g         3486 t
ORIGIN      
        1 tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa aaacattggc
       61 cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg aaggatccgg
      121 tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc aggatgatat
      181 tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt cacatcacag
      241 cgatgccgtt gctgtcgacc tgctcttagt caactgtgat ctggaaaact ccagcaacgg
      301 ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga aaaccaccgg
      361 cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg gcgtacgttt
      421 aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata tcgacctggc
      481 tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa ttgatcaggc
      541 tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg attataaata
      601 agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg tatggacacc
      661 gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc ttgcgcattg
      721 gcggtcgaat tcaatctgaa tgttctcgat aaatcaatgc gcgaccgcat tgatatttca
      781 ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt tgcggtaaat
      841 aataaccaaa tcagtaacga gcaaaagatt aactggcaca aaaatgacga taaaaccatt
      901 ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt ccgtcagtcg
      961 ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct cttcaatttc
     1021 gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc gtggcactca
     1081 gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtgtcct gatggattac
     1141 aacttgtttg ccagcagcta ccgcccacag gatggcagca gcagcactaa tcttaacgcc
     1201 tatggaacag ccggaatcaa cacgggggca tggcgcttac gcagtgatta tcagttgaat
     1261 catactgata gcgatgataa ccatgaacag tcaggcgaaa tatcacgtac ctatcttttt
     1321 cgcccgttac cgcaattagg ttcaaaatta accctcggcg aaacggattt tagtcccaat
     1381 attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgatcg aatgttgcca
     1441 tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa tgccacggtg
     1501 acgatcagtc aatcaggccg cgtcatttac cagaaaaaaa tcccaccagg cccatttatc
     1561 attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac ggaagaagat
     1621 ggtcgggtga acaatttcca ggtttcagca gcatcgacgc ccttcctgac tcgtcaggga
     1681 caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca tcaaactgaa
     1741 aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac ctcgctgtac
     1801 agcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat tgggcaaaat
     1861 atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca ttttgatact
     1921 cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt ggatgccact
     1981 aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca cagctacgcc
     2041 aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca gacgatcagc
     2101 ttatctgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa cctgctacat
     2161 caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg ttttaatgtt
     2221 gatattggtg actggagaga tatctctatt tctacgtcat tcaatacaac ccattacgaa
     2281 gataaagatc gcgacaacca gatttacctg tcgatttcgc tccccttcgg taacggtggt
     2341 cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat gtcgtggaac
     2401 gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc cgaccgtcct
     2461 gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg tgagtgggat
     2521 atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg gagcggttct
     2581 ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa tgaaccacgc
     2641 ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct cgactacacc
     2701 aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc caccgtggcg
     2761 gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat caaagaaacg
     2821 tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa agacggtaac
     2881 gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat ccgccaggat
     2941 gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt aagcggagtc
     3001 gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc gctccatctt
     3061 cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta attaaggaaa
     3121 tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc
     3181 acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat aaagctacca
     3241 gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc tggctcgaaa
     3301 atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata caacgtctgg
     3361 aaccgaaagc aacaacacag gtacggatag taaagcaagc ctcgaccaca aaattaccgg
     3421 gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa
     3481 acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac
     3541 cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag gtcagccagc
     3601 agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt gcctacctgg
     3661 gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg ccttttagta
     3721 cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt tatatggatg
     3781 attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat ttacaagccg
     3841 tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc tgttatttct
     3901 gtcgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg gctcggtaga
     3961 aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg gcgataagat
     4021 ctgggaatct gacgatatta aaataagatc tgggaatctg acgatattaa aattcccgtc
     4081 tattgtgaca acaataccaa cggcaacttt gaaagcgaac atgtctacgt atgggtaaac
     4141 ccctacccgg gggtgcagga tcgttattat caactcggag taacttacaa tggcgttgat
     4201 tatgacgcca accagggaaa aagccgcatc gacactaacc agtgtatcga cagtaagaac
     4261 attgacattt atacccctga tgggatggca gaacaaaatt tgctcgggtg atcccgctaa
     4321 tattcacatg tcgcgaacct ttctcgctcg catgcggtta tacgtcaaaa tacgagaaat
     4381 gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat ttgacggtgc
     4441 cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta ctggtctgga
     4501 aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa cacagatgat
     4561 tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga aaacattcag
     4621 tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag tcagttcctc
     4681 cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg gtaatggtct
     4741 gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat atgctgaata
     4801 cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacagctg aactttcgtc
     4861 cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt ttaaaattaa
     4921 ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt tcagaaaatt
     4981 gctgattttc cccttttcct tgattgctgt tattttttcc tagcccggct ttctttatgc
     5041 tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt ttgcagtggg
     5101 gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta catctcccgg
     5161 cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc ggaattactc
     5221 tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc atgattgcgc
     5281 aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc gtgcttgcta
     5341 ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg cgctatagct
     5401 cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg gcaatggtcg
     5461 ctatggcgca agattacggc gcagatattc gcctggtggc gtttatgcaa tatctgcgag
     5521 tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt gataacgctg
     5581 aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc ctgcttacga
     5641 ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct tccggcacga
     5701 tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc accatcgaac
     5761 taccggaatg gctgctggcg atggcgtata tggcaattgg ctggcggatt ggtcttggtt
     5821 tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg ctgtcgattt
     5881 ttgctctgct ggctatttgt gcaggtatgg cgtggggact gacccggttt atgcatattg
     5941 attttatgac tgcctacctc gccaccagtc ccggcgggct tgataccgtg gcggtcatcg
     6001 ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg cgcctgttca
     6061 gtattttgct gacggggcca gccattgcgc ggtttatttc aacctatgcg ccgaagcggt
     6121 cggcctagtg ctggcagcct ggacaccagt aaaacgggcg cgaggacaac gtggttttct
     6181 caatgatgcc gccacagcgt tcgcacagtt cgccatcacg atgaaaaacc ttaaagcgaa
     6241 acagcgcccc atgatgctta ttctcatcca cctgcccccg cgtagcgtaa gaaaaccgag
     6301 gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga tctttcgctt
     6361 tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa ttgccaagcc
     6421 cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg acggttacga aagcgcggcg
     6481 acaataaccg ttcctttacc acctccggcg tcagattcgg atccagcaca tcgggaccaa
     6541 cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct caacatctca atatccgagg
     6601 cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat actcgcgtgg
     6661 tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga ttatggctgt
     6721 agagcgttaa accgttggga aaatgagtta acaacgcctt accacgcgtt tccacatggg
     6781 taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag gcaaaccaga
     6841 catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca cggcggatct
     6901 ccgggccttc aggcatggtg atatcctttt ttcattcttt attaagcggg gttatgctgc
     6961 gacaacaatc ccctttttgg caaatgtagc tcgtaatcgg cgggcgaacg ccagagcgtg
     7021 ctcgccgtcg ccatgcaggc agaccgtttg cgccgcgacc gttgcccatt cgccggtgat
     7081 acttttgact ctgccgtgtt gcaccatctc cagcgtttgc gccaacgcct gttcttcgtt
     7141 ttcaatcaac gcgcctggct gacttcgcgg caccagcgag ccgtcagcct gataaccgcg
     7201 atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac gaatcagctc
     7261 gcttcctgcc agcccaacga gaaccaatgc cggatcgcaa gcgtataccg ctctggcgat
     7321 ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt gcggtttgac
     7381 atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa tttgatacag
     7441 cgtctgggcg taaacggttt ccggcggcag ctgcatggcg ctgcgaccaa aattttccct
     7501 gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt tttattgctt cacgcacgca
     7561 agcctgcatg gtttgcgcat cgcctgcatg aaatccacaa gcaatattgg cagaggaaac
     7621 cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt tcaggtcaat
     7681 tttcattgtg cagccgccac gctaattgtt caaaataacg ttgctgatct tgccgcgctt
     7741 tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc ggaatttgcg
     7801 ccagatggta catatcagcc tcaatgatac aggcaatacg tggataacca ccggtggtct
     7861 gtgcgtcgtt catcaacaca atcggctgcc cgttatgcgg cacctgcacc acgcccggta
     7921 acaaaccgtg agataacagt tcgcgatcgg tgatgcgttt taaaatttgc ccctgtaaac
     7981 gatagcccat tcggttactt tgcgagctaa gctgccaggg cgaacgccag aatgcatcct
     8041 gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg cggttgcccc
     8101 acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgatttg ccaatcggga
     8161 gtcagtcacc atccttcagt aaacggcctt ccagcccgcc aatccccact ttgagatcgg
     8221 tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc agataactgc
     8281 gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc ggcaatcgcc
     8341 agccggtcca gacggcgtta tcatccaacc gcgcttcgca acctgcaccc gtcagggcaa
     8401 accacccatc agtttcgaac tcaactgtta actgaccgag cgtgatctcc agcgcggggg
     8461 cattggcgtc attacccacc agcaggttag caatgcgcag cgcaggcata tccagtgcgc
     8521 cacagtggct gatacccgac tggcaaaaac cgtgacgacc gccatcctgc acagtggtgt
     8581 acatgcccgc acgaataatc ttcagcatat tccctccttc tgcggtacaa atcgcacgct
     8641 gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc tgagtgaggt
     8701 atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag tctgcggccc
     8761 gccgatcccg acagaacctg ccggaacgag caagcgcggt tcagcgcgcc gtggcgtgtg
     8821 tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta aaaaccagac
     8881 cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctcaacc cgcaatacgc
     8941 cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa tttcaataaa
     9001 gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac gctctatggc
     9061 atccagcgcc agcgactcag gattacgcaa aatcaccgtg atattgttca tgccgggaat
     9121 ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga tccgtttctg
     9181 gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac ctatcagata
     9241 acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga ttaggaatat
     9301 caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat gcgcgaatac
     9361 caccgcgttc agtggcatgg tgacctgcag cataaaaatg caatccctgc tcgcgggctg
     9421 aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca aaacgcgcgg
     9481 cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg accacctcag
     9541 gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc caggaggcta
     9601 gttccattcc cggcacaggc atggtcagct ctccccacgg caccaacggc tcaatttcgc
     9661 ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc tcaggatgtg
     9721 cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac gtttttaaac
     9781 ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag ccatgatgca
     9841 caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg ctggcggtga
     9901 caccggtaac aattttttgc accgtctctt tgccttccac ctgcaaacca ttcggcgcgt
     9961 aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt tcggtgtttt
    10021 tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt acatcgcctg
    10081 ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa aagacgcaaa
    10141 ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag catattgcat
    10201 aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg ccgcgcaact
    10261 attacgtgac gttgccgtca attgtggaat tattatgaat aaacacgcat cacaaccgcg
    10321 cgctatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt acggcatgcg
    10381 tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc acgcctacga
    10441 gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg gctttttggc
    10501 ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga tggcgatcgg
    10561 tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt ccctggcgat
    10621 tatcgtctgc ggctatggcc tgtttaaatc taacgtaagc tgtctgctcg gcgagctgta
    10681 tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcgg cgggtaacgt
    10741 ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca gctgggcgat
    10801 gggctttggc ctggcggcgg ttggaatgat cgcgggtctg gtcattttct tatgtggcaa
    10861 tcgtcatttc actcataccc gcggcgttaa caaaaaagta ctgcgtgcga caaactttct
    10921 cctgccgaac tggggatggc tgctggttct gctggtggca acgcctgcac tgattaccgt
    10981 gctgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta ttggcctggg
    11041 tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcggaagg agctggggct
    11101 gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac agggcggtag
    11161 ctcgattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt ataccgttcc
    11221 gaccgcgatg ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg tgttcctggc
    11281 gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg gaaaatttgc
    11341 tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg cccgctggtc
    11401 cgcaatgtat ggtcactctt ctctgccact gatggtgtta ggcctcgcgg tgatgggctt
    11461 tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg aaattccggg
    11521 cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg cgaactatct
    11581 ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg gggcgatcaa
    11641 ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg cactggcgtg
    11701 tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa accgcgcgct
    11761 ggcgctggag tcataatagg ccagcaccga tgcctgatgc gacgcttgac gcgtcttatc
    11821 aggcctacgt atttcctgca atttattgaa tttgcacaaa tttgtaggct ggattaggcg
    11881 ttcacgccgc atccggcatc tgggctcgat tgcctgacgc gtctgttatt tccccttccg
    11941 cgccgcctca tacgctgcca acgtttgtac tctcgcctct ttgtgctcga ctatcggttg
    12001 cggataatcc agcttcacac ctgctttctg cgcccacttc cacggctcat gcaccgcttt
    12061 ccctggtaca tcgcgcagct ccggtaacca tcgacgaata aactcgccct cacggtcaaa
    12121 tttctcgccc tgagttatcg gattgaaaat acgaaaatac ggcgcagcat cagtacccgt
    12181 agaagccgcc cactgccagc caccgttatt agctgccaaa tcaccatcaa tcagctgcga
    12241 catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca gaaaactggc
    12301 tgtaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac gcatagcggc
    12361 atcaataatc gggtatcccg ttttgccttt ctgccaggcc tgtaaatgtg cgggattgct
    12421 ttgccactgc acacgatccg tccaggcaat aaatggacga tgtttacaca acgaggggta
    12481 atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc agacactacc
    12541 ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac actggcgagg
    12601 cgataacccg cccgttgcca agctggcgga caaacggctg gtgccttcca ctgccggaaa
    12661 atctcgttgt tgctcatact ctccagcacc gttctcgcaa aactggcgta attgcgcaat
    12721 cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag gataattcag
    12781 cgtaatggat ggcgcgggct ttatcgatcc gctactacga acttttggtg cagcgacgca
    12841 ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg taaagacttt
    12901 gtacatctcg tgattgccgg tcatcaccgc ccccggcggc aggatcacgc tgtcatcaaa
    12961 tccttcacac accacgttac gcagcgctct ttcaacgtcc acatcccgcg ctcgctcatt
    13021 cacctcatac tgatagttat aaaaaagatg agtaacgctg ttttccgcgc acacctgttt
    13081 aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag gaataccttt
    13141 ttccgccagc gcgatttgta gcccattcag ttgagcattg ataagttcag cctgacgcgg
    13201 cgacatgtta tgcgtctccc acttgcgtgg tgtagcgata tacagcgcca gcacgcgtgc
    13261 agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta aatcctggcg
    13321 aaaccaggcc agatgggtag tcataaaact cctgaatggc gcaagttaat cacacgcggc
    13381 caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc catatagtgt
    13441 ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact gtcgataagc
    13501 gcagccagct gctggcgttc tgtggaatca atatgcgggc gaaaataacc ctgaacgtgc
    13561 atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag aaagcaaaac aattacgcgt
    13621 tggcggtact ggttgtaaaa atcatccaga ttttgccact gatgaatatc agccacaaac
    13681 gggccaagtt tgcggtactc gggctgagaa tgggccagaa agacgagttt atagcggctg
    13741 tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg taactcatga
    13801 agggcgaaga tacggattag ggtgctgtca tcaaatcgtt gtagattcat tactgctcct
    13861 gaacggtata caggagtaag tgtaggcgtt ggtaaatgat gtttgtgaag ttaatccgct
    13921 agcagggcaa gattacgaaa gcgcgctccc cgcaagcaac taatcaaggt agttaatcat
    13981 ggctgaacac gatattacgc cagaagtcac tatttccaaa acacagcgcc gctataaagt
    14041 cggctatgtc agagcccgcc accaggaccg caaaaccggt atagcccggt attattcaca
    14101 acatcccagt ctgcacctta agggcgactg gctgaaagag gccggatttg ataccggacg
    14161 gggtgtcacg gtgaagattt cggagggggg cctgaccatc atcgctgaca gtgacgaggt
    14221 gcaggaactg cgggatggga tgtttagtgt gctgaacgaa agttaaatat taaa
//
LOCUS       DAZPFP010000092        14153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPFP010000092 DAZPFP010000000
VERSION     DAZPFP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.092"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHUR4_002563"
     CDS             <1..79
                     /locus_tag="ACHUR4_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456110.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUR4_002564"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUR4_002564"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1456111.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHUR4_002565"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHUR4_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1456112.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUR4_002566"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUR4_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1456113.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUR4_002567"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUR4_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1456114.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUR4_002568"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUR4_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1456115.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHUR4_002569"
     CDS             5377..5835
                     /locus_tag="ACHUR4_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1456116.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHUR4_002570"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHUR4_002570"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1456117.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHUR4_002571"
     tRNA            6887..6971
                     /locus_tag="ACHUR4_002571"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHUR4_002572"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHUR4_002572"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUR4_002573"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUR4_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1456118.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUR4_002574"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUR4_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1456119.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUR4_002575"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUR4_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1456120.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHUR4_002576"
     CDS             complement(10252..13995)
                     /locus_tag="ACHUR4_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1456121.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3493 a         3744 c         3399 g         3517 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taa
//
LOCUS       DAZPFP010000093        14110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPFP010000093 DAZPFP010000000
VERSION     DAZPFP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.093"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHUR4_002577"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHUR4_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHUR4_002578"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHUR4_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR1456122.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHUR4_002579"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHUR4_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHUR4_002580"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHUR4_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHUR4_002581"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHUR4_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHUR4_002582"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHUR4_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHUR4_002583"
     CDS             complement(7513..8094)
                     /locus_tag="ACHUR4_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456123.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUR4_002584"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUR4_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1456124.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHUR4_002585"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHUR4_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHUR4_002586"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHUR4_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHUR4_002587"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHUR4_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1456125.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZPFP010000094        13777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPFP010000094 DAZPFP010000000
VERSION     DAZPFP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.094"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            65..172
                     /locus_tag="ACHUR4_002588"
     CDS             65..172
                     /locus_tag="ACHUR4_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1456126.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="ACHUR4_002589"
     CDS             complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="ACHUR4_002589"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1456127.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="ACHUR4_002590"
     CDS             complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="ACHUR4_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1456128.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="ACHUR4_002591"
     CDS             complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="ACHUR4_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1456129.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3967..4155
                     /gene="bcsR"
                     /locus_tag="ACHUR4_002592"
     CDS             3967..4155
                     /gene="bcsR"
                     /locus_tag="ACHUR4_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1456130.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4167..4919
                     /gene="bcsQ"
                     /locus_tag="ACHUR4_002593"
     CDS             4167..4919
                     /gene="bcsQ"
                     /locus_tag="ACHUR4_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1456131.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4916..7533
                     /gene="bcsA"
                     /locus_tag="ACHUR4_002594"
                     /pseudo
     CDS             4916..7533
                     /gene="bcsA"
                     /locus_tag="ACHUR4_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7544..9883
                     /gene="bcsB"
                     /locus_tag="ACHUR4_002595"
     CDS             7544..9883
                     /gene="bcsB"
                     /locus_tag="ACHUR4_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1456132.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9890..10996
                     /gene="bcsZ"
                     /locus_tag="ACHUR4_002596"
     CDS             9890..10996
                     /gene="bcsZ"
                     /locus_tag="ACHUR4_002596"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1456133.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11191..11580
                     /locus_tag="ACHUR4_002597"
     CDS             11191..11580
                     /locus_tag="ACHUR4_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR1456134.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            11662..13650
                     /gene="hmsP"
                     /locus_tag="ACHUR4_002598"
     CDS             11662..13650
                     /gene="hmsP"
                     /locus_tag="ACHUR4_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1456135.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3238 a         3514 c         3754 g         3271 t
ORIGIN      
        1 aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact
      241 ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac
      301 gtacggttta tcttggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt
      361 ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc
      421 atcaagaacg cgaaccacca gatcggagat agccaggaag ctgctcggtt ggtcaatgac
      481 aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc
      541 tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag
      601 cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc
      661 agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt
      721 tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc
      781 actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc
      841 gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat
      901 caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc
      961 gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata
     1021 gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg
     1081 cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaaatc
     1141 aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca
     1201 ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga
     1261 agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt
     1321 aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccggtgcgcc
     1381 acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg
     1441 ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag
     1501 cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac
     1561 taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat
     1621 taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc
     1681 aggcaaccag atgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg
     1741 caagcgatgc aggctgtaac gcggaatggg catcagcaga aacgcggcaa acaccaaatt
     1801 gaggagcggg tggaagttaa gatatcccgc ccacaacagg ccaaacttaa ccagaaaata
     1861 gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt
     1921 ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg
     1981 gccggtttaa cataacgagg tttagcaaag aacaaacgta aggtgtcgcg aatgcgtcgc
     2041 aaagagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gataataatt
     2101 tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca
     2161 tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag
     2221 aactttgcgt taaaggcagc ggcattcccc attgttctgg ggcaagcagg cgcatctgca
     2281 ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc
     2341 cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg
     2401 agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc
     2461 gacatagcgt cagggcttgc tcaacgcgga tacccggtac cggacgtagg gcaaccagaa
     2521 cgcctttacc gtgggcaggc aagagagggt tattcatcat gttgttgacg gcattacaga
     2581 acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag
     2641 tgatatcttc cggtacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca
     2701 gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca
     2761 aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac
     2821 taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt
     2881 gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt
     2941 cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa
     3001 cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa
     3061 ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt
     3121 tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg
     3181 caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat
     3241 ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat
     3301 ccaaccatga gcgaagccgc tcggcaggaa tattttgcca tgcgttattt gcgcaaacaa
     3361 gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata
     3421 gacctttttc atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat
     3481 ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt
     3541 cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt
     3601 taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac
     3661 cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt
     3721 tactagcgaa agctaagaaa taaacctacc attgaaatta aaacttatca gatttagcat
     3781 gtgaatcaat ttagaaggtc acaagacgtg tgattttagt cttttcgttt ttaacttttt
     3841 ctgttgcgta acggaaagtc aaaaagtgag caaattcccg tctcgccgta aacaataaac
     3901 cggcgaaatg ctcacggtta gaattgtctc atgaatggta cggttatttc atagggatca
     3961 agcaaaatga ataacaatga accagatact ctgcctgatc ccgcgatagg ctatatcttc
     4021 cagaatgata ttgtggcgtt aaagcaggca ttttcactgc ctgatattga ttatgccgat
     4081 atttcccaac gcgaacagtt ggccgcggca ttaaaacgct ggccgttgct ggcagagttt
     4141 gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg attgcagggg gtgcggggag
     4201 gcgtggggac aacaaccatc accgccgcat tagcctggtc attacaaatg ttgggagaaa
     4261 atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg cctgtcattt aatgttgatt
     4321 ttacccaccg tcagggctgg gccagagcga tgctggatgg ccaggactgg cgtgacgctg
     4381 ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg tcagttatcc attgaagaac
     4441 aagaaaatcc acagcactgg caaacccggc tgagcgatat ttgctccggc ttacagcaac
     4501 taaaagccag cgggcgttac cagtggattt taatcgactt accgcgtgat gcctcgcaga
     4561 taacccacca gctgctgagt ttgtgcgagc actcgctggc aatcgtcaat gtggatgcca
     4621 actgccatat ccgactgcat cagcaagcgc tgccggatgg cgcacatatt ttgattaatg
     4681 acttccgtat tggcagtcag gttcaggacg atatttacca gctttggttg caaagccagc
     4741 gccgattact gccgatgctc attcatcgtg atgaagcgat ggctgaatgc ctggcggcta
     4801 agcaaccagt aggtgaatat cgcagtgatg cgctggcggc tgaagagata ctgacgctgg
     4861 cgaactggtg cctgttgaac tactccgggc tgaaaacgcc agtcgggagt gcatcatgag
     4921 tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg cggcttatcg ggcgttatcg
     4981 cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg ctcggctgtt tctggatgat
     5041 cctggcctgg atttttattc cgctggagca cccgcgctgg cagcgtattc gcgcagaaca
     5101 taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt ccgctggacc cggtccgtta
     5161 tctcattcaa acatgctggt tattgatcgg tgcatcgcgc aaagaaacgc cgaaaccgcg
     5221 cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt accatcaatg gatgaacgag
     5281 ctgcctgagc gcgttagcca taaaacacag catctggatg aaaaaaaaga gctcggtcat
     5341 ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg
     5401 gcgttaatct gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg
     5461 ctgtgggggg tagcgctgat cgtacggcgg atgccggggc gcttctcggc gctaatgttg
     5521 attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac ctctacgctg
     5581 aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac
     5641 gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg
     5701 gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt cccgacttac
     5761 aacgaagatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat cgactggccg
     5821 aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt tcgccagttt
     5881 gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc gaaagcgggc
     5941 aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac
     6001 cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa agagaaacag
     6061 ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga acgcaacctg
     6121 ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt gcaggatggc
     6181 aacgatatgt gggacgccac tttcttctgc ggttcctgtg cggtgattcg tcgtaagccg
     6241 ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca tacttctctg
     6301 cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc ggcggggctg
     6361 gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggta
     6421 taaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc tcagcggctg
     6481 tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact
     6541 gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc
     6601 gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa gatccagggc
     6661 aaatatcgcc actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca
     6721 ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa
     6781 ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt
     6841 gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca
     6901 accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct gattgttctt
     6961 ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgaa ccgcgtggag
     7021 atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag
     7081 gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg
     7141 cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg
     7201 gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat
     7261 atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc
     7321 tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc
     7381 taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact
     7441 tctctggttt cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca
     7501 caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt
     7561 ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac
     7621 gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga cggaacaaaa
     7681 tccgcaggtg gggcaagtga tgtcaggcga gcagggagct gatgccccca tcgtggcgca
     7741 gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa atcgcgccgc cgccgggcag
     7801 catggtgcta cgtggtatta acccgaacgg cagcattgag tttggtatgc gcagcgatga
     7861 agtggtgacg aaggcgatgc tcaacttcga atacacgcca tcgccatcgt tactgcctgt
     7921 ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa
     7981 agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aatccactgt ttattaccga
     8041 cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc
     8101 cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac
     8161 cctgaatgtg aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgcgataa
     8221 ccgtactaac accttgccga tggtctttgc gggtacgccg gatgttgggc tgcaacaagc
     8281 atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc
     8341 ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca acgacaaacg
     8401 gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca
     8461 tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt
     8521 gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt
     8581 gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtacgtac
     8641 cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac aatccagcgg
     8701 ccttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct acctgatgcg
     8761 cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag
     8821 ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagcaa
     8881 acaggaggcg aaccgtctgc tgctgcgtat tccggtatta caaggtttgc tggatggcaa
     8941 aacagatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt
     9001 tgagtatatg aacccgatgc caggcggttc ggtagataac tgtattacct tccagccggt
     9061 gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt attaccactt
     9121 catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccattca gccggatggc
     9181 ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct aacgaagcac agatggaaac
     9241 gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac
     9301 ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg
     9361 tatcccggac aaactgaaag acgataagca gatcgaccta ttggtgcagg cgaccgaaag
     9421 ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga
     9481 tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg
     9541 cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgttggcag atagcccacg
     9601 cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg
     9661 ttcggtcgcg gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta
     9721 cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct
     9781 gctggcggtg ctggcgacta tcagtgtgat attgctggca tgggtactgt ggcgtctgct
     9841 gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt
     9901 gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt agtgttcagg cagcctgtac
     9961 ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga
    10021 ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta tgttctttgc
    10081 cctggcggct aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct
    10141 cgctcagggt tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag
    10201 taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcctggtc
    10261 gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt
    10321 gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt
    10381 accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacatgcc
    10441 gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac
    10501 caatcaacat ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta
    10561 tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc
    10621 tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat
    10681 gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa
    10741 agtggatgtg gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat
    10801 gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag cgccagcgcg tggccgataa
    10861 ctttcccggc agcgatacct attacaacta tgtgctgacc ctgtttggac aaggctggga
    10921 tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg
    10981 cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc
    11041 gaggcagcac caaccatcag cagaccctgc ggatggacag cggcaacact aatgccgtgc
    11101 gcgggctggc gaatatttac cgccagcagt cgccagaaaa agctgaagcg tttatcgcct
    11161 cgctctctgc cagccagcgg cgcagcattg atgatatcga acgcagcctg caaaacgacc
    11221 gtctggcaca gcaggcagag gtactggaaa accagggcaa atgggcgcag gcggcagcac
    11281 ttcagcggca acgactggcg ctgggccccg gcagcgtatg gattacttac cgactggcag
    11341 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggcttcgg ctacacggcg
    11401 cgggcattac ttgaacgacg tgttacttcc aactggtttg ttggcacggc aattgatatc
    11461 cagcaggtga aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
    11521 ggatggcagg gtgacatgga tttaccgccg cagccgctga taccttacgc cgactggtaa
    11581 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    11641 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    11701 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    11761 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    11821 cgcgaaccct tatcttcagc tattttgaaa ggcgatattc ccgaagcgga agctattctt
    11881 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc taaccagttc
    11941 caggcgctgc gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg
    12001 tttgagctac cggttcaaat ctcgctgggc gtctactcgc tcgaacgtcc ggcaaatccg
    12061 cagccaatag cctatctggt gctacaggcg gattccttcc gtatgtataa gttcgtgatg
    12121 agcactctct caacgttagt gaccatttac ttacttttgt cgcttatcct gaccgtcgcc
    12181 atcagctggt gcattaaccg cctgattttg catccgttac gcaatattgc tcgcgaactt
    12241 aacgccatcc cagcccagga gcttgttggt caccaactgg cattaccgcg tctgcatcag
    12301 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    12361 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    12421 gccttgctga tggagatgct ggagcaggtt gtcgcgcgta aacaaaccac cgcgctgatg
    12481 atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc tgaaagaggc gcaacgagaa
    12541 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg tatgatcctc
    12601 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    12661 gcaatcactt tgggtcagca agtactcact atcatgagcg agcgcctgcc gattgaacgt
    12721 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    12781 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaagaat
    12841 cagattcagt tctttgatcc gcagcagatg gaagcagccc agaagcggtt gacggaagag
    12901 agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa tttggttaca gccacaggtc
    12961 gagatgacca gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    13021 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    13081 accgtcggtc actgggtgct ggaagagtcc tgtcgattgc ttgcagcctg gcaagagcgc
    13141 ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc aactgatgca cccgaatatg
    13201 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    13261 gaagtgacag aaagccgacg tattgacgac cctcatgctg cggtggcaat cctccgtccg
    13321 ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg
    13381 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    13441 gttgaaggct tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    13501 ctgaacttac aaatgattgc cgaaggcgtg gagactgaag cacaacgaga ctggctggca
    13561 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    13621 ttcgaagaga gttacctgga agaaaagtag ctaccccaaa ctgattacaa aactttaaaa
    13681 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    13741 ggtacatttc tgtgatgttg tctgggtgtt attttaa
//
LOCUS       DAZPFP010000095        13558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPFP010000095 DAZPFP010000000
VERSION     DAZPFP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.095"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACHUR4_002599"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACHUR4_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1456136.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUR4_002600"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUR4_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1456137.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUR4_002601"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUR4_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1456138.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUR4_002602"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUR4_002602"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1456139.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUR4_002603"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUR4_002603"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1456140.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUR4_002604"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUR4_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACHUR4_002605"
     CDS             complement(5911..6954)
                     /locus_tag="ACHUR4_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1456141.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACHUR4_002606"
     CDS             complement(7200..7652)
                     /locus_tag="ACHUR4_002606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1456142.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUR4_002607"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUR4_002607"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR1456143.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUR4_002608"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUR4_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR1456144.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUR4_002609"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUR4_002609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1456145.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUR4_002610"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUR4_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1456146.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACHUR4_002611"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACHUR4_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUR4_002612"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUR4_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1456147.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="ACHUR4_002613"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="ACHUR4_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3376 a         3517 c         3393 g         3272 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacattcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacggga
//
LOCUS       DAZPFP010000096        13460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPFP010000096 DAZPFP010000000
VERSION     DAZPFP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.096"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..2212
                     /gene="crfC"
                     /locus_tag="ACHUR4_002614"
     CDS             <1..2212
                     /gene="crfC"
                     /locus_tag="ACHUR4_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1456148.1"
                     /translation="YELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPN
                     IAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTL
                     IRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIEN
                     GVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAG
                     LESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREA
                     ILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYL
                     ANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSL
                     FAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIH
                     QVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQDDSEPLS
                     EIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELSESGFRP
                     GFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNE
                     KSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQ
                     TRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAERY"
     gene            2209..3087
                     /locus_tag="ACHUR4_002615"
     CDS             2209..3087
                     /locus_tag="ACHUR4_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR1456149.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3351..4853
                     /gene="proP"
                     /locus_tag="ACHUR4_002616"
     CDS             3351..4853
                     /gene="proP"
                     /locus_tag="ACHUR4_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1456150.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4965..5054
                     /gene="pmrR"
                     /locus_tag="ACHUR4_002617"
     CDS             4965..5054
                     /gene="pmrR"
                     /locus_tag="ACHUR4_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1456151.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5030..6130)
                     /gene="pmrB"
                     /locus_tag="ACHUR4_002618"
     CDS             complement(5030..6130)
                     /gene="pmrB"
                     /locus_tag="ACHUR4_002618"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1456152.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6131..6799)
                     /gene="basR"
                     /locus_tag="ACHUR4_002619"
     CDS             complement(6131..6799)
                     /gene="basR"
                     /locus_tag="ACHUR4_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1456153.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6796..8439)
                     /gene="eptA"
                     /locus_tag="ACHUR4_002620"
     CDS             complement(6796..8439)
                     /gene="eptA"
                     /locus_tag="ACHUR4_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1456154.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8543..9880)
                     /gene="adiC"
                     /locus_tag="ACHUR4_002621"
     CDS             complement(8543..9880)
                     /gene="adiC"
                     /locus_tag="ACHUR4_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1456155.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10017..10778)
                     /gene="adiY"
                     /locus_tag="ACHUR4_002622"
     CDS             complement(10017..10778)
                     /gene="adiY"
                     /locus_tag="ACHUR4_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1456156.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11103..13370)
                     /gene="adiA"
                     /locus_tag="ACHUR4_002623"
     CDS             complement(11103..13370)
                     /gene="adiA"
                     /locus_tag="ACHUR4_002623"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1456157.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3238 a         3295 c         3545 g         3382 t
ORIGIN      
        1 gtatgagtta agtcaggagg ctgaacgcct gttacagctt tctcgccaac agttgcagtt
       61 actggaaaaa atgcctctct ctgtacccgg agacgacgcg ccacaactgg ctttaccctg
      121 gagtcagcct aatatcgccg aacgtcacgc gatgctgaat aatgagttgt gtaaaatttc
      181 ccgactggaa atggtgctgg caattgtcgg taccatgaaa gcagggaaat caaccaccat
      241 taatgccatt gttggtacgg aggttctgcc taatcgtaat cgcccaatga ctgcgctgcc
      301 gacgcttatt cgccatacgc ccgggcaaaa ggaaccggta ctgcattttt cacatgtcgc
      361 gccaatcgat tgtttaattc aacaattaca acagcgcctg cgtgattgcg atattaagca
      421 tctgaccgat gtgctggaaa tagataaaga tatgcgtgcg cttatgcagc ggatcgaaaa
      481 tggcgtcgct ttcgaaaaat attatctggg tgcccagcct atttttcatt gtctgaaaag
      541 tttgaatgat ttagtgcgac tggcgaaggc gctggacgtc gattttcctt tttctgctta
      601 cgccgccatt gagcatattc ccgtgattga agtggagttt gtccatctgg cggggctgga
      661 aagttatccc ggtcagttga cgttactgga tacccccggg ccaaatgaag ccgggcaacc
      721 gcatctgcaa aaaatgctta accagcagct ggcacgcgcc tcggcggtac tggcggtgct
      781 ggattatacg caactgaaat cgatctccga tgaagaggtc cgtgaggcga ttttggcggt
      841 ggggcaatcg gtgccgctgt atgtgctggt caataagttc gatcaacagg atcgtaacag
      901 tgacgacgcc gaccaggtgc gggcactgat ttccgggacg ctgatgaaag gctgtattac
      961 gccacagcag atattcccgg tgtcgtcgat gtggggctac ctggcgaatc gggcgcgcta
     1021 tgagttagcc aacaacggta agttaccagc gccagagcaa caacgctggg tggaagattt
     1081 tgcccatgcc gcgctcggca ggcgctggcg tcatgccgat ctggcggacc tcgaacatat
     1141 tcgtcatgct gccgatcagt tgtgggaaga ttcgctgttc gcccagccaa ttcaggcgtt
     1201 gcttcatgcc gcttacgcta acgcctcatt gtatgctctg cgatctgctg cgcataaact
     1261 gttgaattac gcgcagcagg cgcgggaata cctggatttt cgtgcgcacg ggttaaacgt
     1321 cgcttgtgaa caattgcggc aaaatatcca tcaggtcgaa gaaagtttgc agctattgca
     1381 actcaatcag gcgcaggtga gcggcgagat taaacatgaa atcgagctgg ccctgacctc
     1441 cgccaaccac tttctgcgtc aacagcaaga tgcggtgaat gcgcagttag ccgccttgtt
     1501 tcaggatgat tcggagccgt taagcgagat tcgtacccgc tgtgagacac tgttacagac
     1561 ggcgcagaac accatcagtc gcgactttac gctgcgtttt gccgagatgg aatccaccct
     1621 ttgccgggtg ttaaccgatg ttattcgccc cattgagcaa caagtcaaaa tggaattgag
     1681 cgagtcaggg tttcgtcctg ggtttcattt tcctgttttt cacggcgtag ttccccactt
     1741 caacactcgc cagctgttca gtgaagtcat ttcgcgccag gaagcaacgg acgagcagag
     1801 cacgcgttta ggcgttgtgc gtgagacttt ttcgcgctgg ttgaatcagc ccgactgggg
     1861 acggggaaat gagaaatccc cgacagaaac ggttgattac agtgtgttgc aacgagcatt
     1921 aagcgcagaa gtcgatctct attgccaaca aatggctaaa gttctggcag agcaggtcga
     1981 tgaatctgtt acggcaggca tgaatacttt tttcgctgag ttcgcttcat gtttgacaga
     2041 attacagacg cgtttacgcg aaagtctggc tctgcgtcaa caaaatgaat cggtggtcag
     2101 gctgatgcag cagcaattgc agcagactgt gatgactcac agctggattt acaccgacgc
     2161 tcagctgtta cgcgatgata ttcaaacact tttcacggca gaacgatatt gaccaagacg
     2221 ttactggacg gccccggtcg cgtgctggag tcggttcatc cgcgcttttt agtggatctg
     2281 gcgcagggcg atgatgcccg ccatcctcaa gcccatcagc agcagtttcg tgaacgactg
     2341 atgcaggaac ttctttcgcg tgtgcagctt cagacatgga cgaacggcgg catgttaaat
     2401 gcgccgctta gcctgcgact gacaatggtg gaaaaactgg cgtcgatgct ggatcccggt
     2461 catctggcgc tgacgcagat cgcgcagcat ctggcgctgc tgcaaaaaat ggatcaccgc
     2521 cagcactctg ctttcccgga gctcccccag caaattgccg ccttgtatga gtggttttca
     2581 gcccgttgtc gctggaagga aaaggcgtta acgcaacgag gcctactggt gcaggcaggt
     2641 gatcagagcg agcaaatttt tacccgctgg cgtgctgggg cgtataacgc ctggtcgttg
     2701 cctgggcgct gttttatcgt tctggaggag ttgcgctggg gtgcatttgg cgatgcctgc
     2761 cgtctgggaa gcccgcaagc ggtggtgttg ttgctgggtg atttgcgcga gaaagcgaca
     2821 caacatctgg cagagagtat caatgcggca ccgactacgc gtcactatta ccatcagtgg
     2881 tttgcctctt cgaccgttcc gacgggcggg gatcatgctg attttttaag ttggctggga
     2941 aagtggacca cggcagataa acaacccgtt tgctggtcag tgacccaacg ctggcaaact
     3001 gtcgcgctgg ggatgccacg actctgttca gcgcagcgtt tggtgggggc aatggtcgag
     3061 gaaatcttct ctgtaaattt ggcgtaaata atcagttaca tcaatgagtc ctaaacgaaa
     3121 tccatgtgtg aagttgatca caaatttaaa cactggtagg gtaaaaaggt cattaactgc
     3181 ccaattcagg cgtcaactgg tttgattgta cattccttaa ccggagggtg taagcaaacc
     3241 cgctacgctt gttacagaga ttgcatcctg caattcccgc tccccttttg cggccgtcgc
     3301 gctgattttt ctggcgtttg cggaaatggg ccaactctgc gaggaaagct atgctgaaaa
     3361 ggaaaaaagt aaaaccgatt acccttcgtg atgtcaccat tattgatgac ggtaaactgc
     3421 gtaaagccat taccgcagca tcactgggta atgcaatgga atggtttgat tttggtgttt
     3481 atggttttgt tgcttacgca ttaggtaaag tttttttccc gggggctgac cccagcgtgc
     3541 agatggttgc tgcacttgcc actttctccg ttccctttct gattcgaccg cttggcggac
     3601 tcttctttgg tatgttgggc gataaatatg gtcgccagaa gatcctcgct atcactattg
     3661 tgattatgtc gatcagtacg ttctgtattg gcttaatacc gtcctacgac acgattggta
     3721 tttgggcacc gattctgctg ttgatctgta agatggcaca aggtttctcg gtcggtggtg
     3781 aatataccgg ggcgtcgata tttgttgcgg aatactcccc tgaccgtaaa cgtggcttta
     3841 tgggcagctg gctggacttc ggttctattg ccgggtttgt gctgggtgcg ggcgtggtgg
     3901 tgttaatttc gaccattgtc ggcgaagcga acttcctcga ctggggctgg cgtattccgt
     3961 tctttattgc tctgccgtta gggattatcg ggctttacct gcgccatgcg ctggaagaaa
     4021 ctccggcgtt ccagcagcat gttgataaac tggaacaggg cgaccgcgaa ggtttgcagg
     4081 atggcccgaa agtctcgttt aaagagattg ccactaaata ctggcgcagc ctgttgacat
     4141 gtattggtct ggtaattgcc accaacgtga cttactacat gttgctgacc tatatgccga
     4201 gttatttgtc gcataacctg cattactccg aagaccacgg ggtgctgatt attatcgcca
     4261 ttatgatcgg tatgctgttt gtccagccgg tgatgggctt gctgagtgac cgttttggcc
     4321 gtcgtccgtt tgtgctactt ggtagtgttg cactgtttgt gttggcgatc ccggcgttta
     4381 ttctgattaa cagtaacgtc atcggcctga tttttgccgg gttactgatg ctggcggtga
     4441 tccttaactg ctttatgggc gttatggctt ctaccttgcc agcgatgttc ccgacgcata
     4501 tccgttacag cgcgctggcg gcggcattta atatttcggt gctggttgcc ggtctgacgc
     4561 caacactggc ggcctggctg gtcgaaagct cgcagaatct gatgatgcct gcctattacc
     4621 tgatggtagt ggcggtgatt ggtttaatca ccggcgtaac catgaaagag acggcaaatc
     4681 gtccgttgaa aggtgcgaca ccggcggcgt cagatataca ggaagcgaag gaaattctcg
     4741 tcgagcatta cgataatatc gagcagaaaa tcgataatat tgaccacgag attgccgatt
     4801 tgcaggcgaa acgtacccgc ctggtgcagc aacatccgcg aattgatgaa taagctgaaa
     4861 cggatggcct gatgtgacgc tgtcttatca ggccaattga actcttaagg ttcacttaat
     4921 ctctgacgcg catactctcc tccaggttaa cggaggagag tgcaatgaaa aaccgtgttt
     4981 atgaaagttt aactaccgtg ttcagcgtgc tggtggtcag cagctttctt tatatctggt
     5041 ttgccacgtt ctgatctttc ttcagccgta cccaggcccg cgtgccggaa gtctcttgcc
     5101 ggttttgcag gaaaaactgc ccgtgatgca actgtgtaat gcggctgaca atacttaacc
     5161 ccagaccaat cccgccataa cggctgtcca tacgtacaaa cgctttactc aactccccgc
     5221 atttactctc atcaatacct ggtccttcat cttcaactgc catgaccgct ccgccatctt
     5281 cttgcagctt aatcataatg ttgctgcctt gcgggctgta acgatgggcg ttttctacca
     5341 ggtttcgcaa taacatccgc agcagggttg catcaccctg aacggtgatg tcggcggcgc
     5401 tctctggcaa tagcagggtt tgctgtcgct ggtcgagcat ggtactgagt tcgtcatacg
     5461 aggggagaat gacatcttcc agcagtttta catgttgata attaccggaa gaaaatgact
     5521 gtccggcacg cgccagttgc agcagctggg agacgctctc catcatctga tcaagccgtg
     5581 ccactaacgg tgctacatca atgtgatgcg ttttcgccag cagttccaga tgcaaacgca
     5641 cccccgccag tggcgttcgc agttcgtgcg cgacgtcagc ggtaaacaac ctttcgttat
     5701 ccagcgtgct ggtcaggcga ctgaccagat cgttcaacgc cgaaaccacc gcttcgattt
     5761 cgagggtggc gctgtgaatg gcaatgggcg ttaagttgtc ggcggtgcgc gcttccagct
     5821 ctttttgcag ctccgccagc gggcgggtga tgcggcgtac cgcctgatag cagataaata
     5881 gcgtcaggct gaccataaag acgccgggga caatcaggct ggcgaccgcc tcgcggatct
     5941 cacgcatgat gtggcgatcg ttgttgcgat tgtcgcgtag cgcctgctca aacagctgaa
     6001 tctgctcggt actttcatgc catagccaga agacgctgat cagctcaaac accaacaaaa
     6061 tggccccgat ggtcaatatc agccgttggc gcagcgatat aggtcggcgc agaaaacgca
     6121 tcagattcaa ttagttttcc tcattcgcga ccagcatata gccaaagccg cgcacggtgc
     6181 ggatacgggc tttgcccact ttgtcgcgca gattgtggat atgcacttcc agggtgttgg
     6241 tcgagggttc attgtcccag ttatagatgt cgttgtagag aatttcccga tgtaccggac
     6301 tgcctgcttt gagcattaac cgtgacagca gagcatattc tttgggcgtc agaatcaact
     6361 cttcaccgcc catccatacc tgacggcgac ccatattcag cgtcagattg ccaacaatca
     6421 gctcactttc gccctgatta ttatggcgtc gtagcagggc gcggatacgg gcgtgtaact
     6481 cttccagcgc aaaaggcttc accagatagt catcggcacc gacatccagc ccggcgattt
     6541 tgtcggtcag cgtatcgcga gcggtgagga tcagtaccgg cagggtgtat tttttctgcc
     6601 ggatacgggc gagaaaatgc agtccatctt cgtcgggtaa ccctaaatcc agtaccacca
     6661 ggctgtaatg accgtcctca aggctttgtt ccgccatccg cgcggttgtc acgccatcgc
     6721 acgcgtagcc ttcggtttgc gccgccagaa tcagtccctg caataacagc gtatcgtctt
     6781 caacaatcag aattttcatt tactcactct cctgcaagtt tgcagaatat catccgcggc
     6841 ctggtaatac ttcgtctcaa cgccagttaa tcccaatagc gtggagaata aattgtcttg
     6901 tgaatagtgt tgcgtttgcg cctgtttttg caggcagttc tggtcaacct gataccgctt
     6961 ttgataatcc tccgacagcc acagcagcat tggcacctgt ttttggctat ccggggcgat
     7021 ggcataaggc agaccgtgca gatagatgcc attttcacct aacgattcac cgtggtcaga
     7081 aagataaacc aggctggtgg taaatttatc ctgatgttct ttaagcagat taatcgcttt
     7141 atcaacaata tagtcgacgt aaaccagcgt gttgtcgtaa gtgttcacca gttgctcttt
     7201 ggtacaggtc tggatctcat tggtgtcgca ggttggggta aatttcctga actgcggcgg
     7261 atagcggttg taataggtcg gaccgtggct gccgatggtg tgtaagacaa tcacgccatc
     7321 accctgcagg ttattgatgt actcttccag cccgtggaac agcacttcgt catagcattc
     7381 gccgttgatg cactgatcag gcagattcag cgcggtgacg ttctggtgag gcacgcggtc
     7441 gcaggcacct ttacagccgc catcgttgtc attccacagc acgttgatgc ccgctcgctg
     7501 aatgatatcc agcacgcctt cctggtgctg tgccagctct tctttgtagt gctcacgcgg
     7561 catatccgag aacatgcacg gtactgaaac tgccgttgcc gtgccgcaag atgcggtatt
     7621 agggaaatag accacgttat ctttcgccag ccgcgggtta gtttcacgcg ggtagccgtt
     7681 gagggagaag ttctccgccc gcgaggtttc gccgacaatc aggatggtca aatttttacg
     7741 tttttcgttc tgcattaacg ggttgcggtg cgcgtcttca ccaattcgca ccagcggcag
     7801 atttgccagt cgctgatggg agtaccatga ccagctggca acaatgctgt tagaggggct
     7861 taaggatttc accagctctt tgttattgcg gaacaacgag gcgtagtctt tataaaacag
     7921 tgcggcgacc agcaaaatca gcagtacaga aaccagaata ttggctccac ggaaaagaac
     7981 actgcgcaga cgcgaggtgg caggtttgat ttttagccag caggcaatca gctcagcaag
     8041 cacgccgctg aatcccagcg ttaataacat ttgcggtgtc atcagcgcat aactttctgc
     8101 tggagtggta tcaataatat tggcaatcat cgagcggtcg atgacgatgc cgtaagtcat
     8161 tatgaaatat tgtgcagccg cgccaatcag aataaacagg caggccagtg gtcgattaag
     8221 ccataagaaa gagcttagtg tcaggacaat attaatcacg ctgaaagcga caaccggcat
     8281 cgacaagaaa accagtacgt tatgcagcga atccagcggc agcgcctgca acacctgttt
     8341 aaaaaaggca atattcaggc agatagagat ataaaaagcg gccaacaata gccaggcgag
     8401 taaattcaaa gagggtcttt ttagtaggcg cttcaacatc acggtgtttc catcgaacaa
     8461 agtgcgcata tgctcgcaaa gcaaaattaa gccaacctta agttcttaag gttggctttt
     8521 atgtttgctg gattaagggg aattaatctt tgcttattgg tgcatctaag ggatacgggt
     8581 ttttatgtag ccggttgtaa ttcagcgcat acatggcggt gatgaccatc agggtgacaa
     8641 atgaccacat aacctctttc gccccggacc ccaccacggc ccaaatgcag tagaggaagg
     8701 caatggtagt aacagccaga tatgccgggc gtgctttacc aaagtgaccg tggccgagca
     8761 gcagtaacgc cgcacaggtg taaagatatg gcaccagtgt aaagatgacc gagacggaag
     8821 aaaccagacc gaactctttg gtcgcgtttg gtgaaatgct gctgagctgg aagatggtca
     8881 tcaaaatacc gacgataatc agccccgcca ctggcgtacc cgctttattt acacgggcaa
     8941 aaatcggtgg gaacagtccg tcatcggcag cggctttcgc cgtttgaccc gccagcaacg
     9001 tccagccgcc cagtgaacct aagcaacccg cagctgcgca gaaggaaaca atggccccag
     9061 cggtgtcacc caacgccatc cgtgcggcat caccgaatgg cgaggcagaa acgcgcagtg
     9121 cggcattagg gatcatcccc ataatcgcgg tggtggagag cacatagcaa acggcggcaa
     9181 tcaatacccc accaatggtg gcgataggga cattgcgttt cgggtttttc accacacctg
     9241 cggcaacgga ggcactttcc acaccgatga acgaccacag cgtaacgtta agggtacttt
     9301 gaatcgcacc gaaggtgccc aggccgctga cgttccatgc cgccatatag gtttcaccac
     9361 ggaaccagaa ccagccaaat acggcaatcc cgacgatggg gatcagcgcc agcacagtgg
     9421 caacggcctg cacgcgggtg atcattttcg gaccgacaat gttcagcagg acgaagatcc
     9481 acagcaccac gacgcaggtg atggttaata ccaatggatc tttcagaatc gggaagaagt
     9541 aacttaaata tcctacgcca atgaccacca tggcgatatt gccgatccag caggccagcc
     9601 agtagaggac gttggtttga taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt
     9661 aagaaccacc aggacttggg tcgaggaacg acattttggc gtataccatc gagagcccca
     9721 gcgcaccgat aatcgtcacc aaccatccat aaatggcaat cccgccagtg gaggccaggt
     9781 ttgcaggtaa cagaaaaaca cctgacccca taatattccc cgacaccatc agggtgacgg
     9841 ggattaagcc cactttgtga gcatcagcat ccgaagacat aattaaactc ctgcgaaggc
     9901 gagcttcgtg acaataaatt acgtcatatc atacgcctgc attcgatagt tgaattatta
     9961 tcgaccgggt tatcgctgat gccgttatta atatagctct tcgttaaggt taaaaatcag
    10021 gcgacagtgc gttctctgtg ctgactaaca taatgcagcg gcgtcatacc gtagaagtct
    10081 ttaaagacag aaataaagta cgacgtactg ttgtagccgc aggactgtga tacctgagag
    10141 atatttttac cgtccatcat taattcattt acggcataac gcatgcggca ggtggtgatt
    10201 atttggctat aactggtgtt ttcgcttttc aactttttct ttaacagact tgggctaaga
    10261 cataaacaac tggctaccat actaagattc cagtctttgt gaatatcgct ttcaataatt
    10321 tgataaacgc tgtcacttac acaattacgt aacatataca tcattaatga aaggaatttt
    10381 ttactgtcga gaaaacggga tagcaccgtg aataataatg cgcgtgttcg ttccttttct
    10441 gactcagttt ctgcgggcat catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc
    10501 aggcatggca gggtgagtat gggcgtagcg ctacgttgcc atactggtat ttgcgagaga
    10561 tctttattca gaaaccggag gtaatctttg atgatgtcgt gactaatatg agcaaccaaa
    10621 gtgttgttaa gagaggagac gtcgataata ttattttcac aatttaataa cgccatatgg
    10681 ttagctttaa ggctaatagg ttctttccca ttcaccctta tccagacatc tttttcagtc
    10741 aataaaacaa tacaaggttg gtcgctgcaa atcctcatac atgtactcct gagtgcgaat
    10801 aaaagaccag ttctcgctaa agcaaagcga taccgggaaa tggttatata attgcatgat
    10861 gaatataaac atgcataaaa ataacgagtt ttactaatat tacaaatttt tatagtaatt
    10921 tctcatgtac atattttttt acaggataaa taacaggcaa aaaaaagcgt ggggtggggc
    10981 cgcgcaaaaa gtatgacatt tttgtttgca gcaattttta tgaaaacgtt gccatactca
    11041 aaaggcctaa ggccaggcgg cgtaatgtta tttaaacaat tacgccttca gcggaatagt
    11101 ggttacgctt tcacgcacat aacgtggtaa ataccgtcaa taatttcagt cccttcagtt
    11161 tcgtgttcaa atctagggaa atggtggtcc caggattgca gcgagcgtaa ataacttact
    11221 tgcggactgt ttttatcgcc gaagttttca ccagacagca gcatcgggat tcctggcgga
    11281 tacgggataa ctgagtttgc cgcgatgcgt cctggcagat tttcaatgga taccagttcg
    11341 acattgttgt cgacaatcgc gttgtacgct tcacgcgggg tgatttccgc caccggcagg
    11401 ccggaatagg cttcgttcaa ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg
    11461 gtgtcaccca gatcgtgaat ccccatgttc gcgtaagtgt caggatattg ttcaacaagt
    11521 tccggcatca cctgcgccag cggtgtgttg gcgtcatagt ggtgtttgaa ggagcaaagg
    11581 gtgttaacca gagtccccca tttcccacgg gttacgccca tagagaacag gaacataatt
    11641 tggaagtcag tggtgcgggt aggcacaatg ccgtggcgac caagccaggc agtgaccagc
    11701 gccgccggaa caccggtttc ttccagttca ccatcttcac ccattcccgg agcaaggatg
    11761 ctgactttaa ttgggtcgag catactccag ttatccggaa tatctttgaa gccgtgccag
    11821 ctttcgcccg gatgcattac ccagcagtcc tgaacggtgg tcagcagttt ggttggtgcg
    11881 tcagcaaagt cataggtttt gccggtttgt gggtcggtga cgacttcttt gttccacggt
    11941 ttgaagaacc agctaccgtc agcggtgaac tctttatata gccgcgccat cgcctgacgg
    12001 aaatcaaccg cttcgtcaat cacttcctgt gtcagtgaca ggccgctgtt gccgtccatc
    12061 atcgacaccg ccacgtcgtt ggatgcgcag atggcataca gcggggaggt ggtggcatgc
    12121 atcatgtagg cctggttgaa gcgggagaag ttaatcgccc cacgaccttc acgtacatga
    12181 atataagaag cctgtgacag cgcattcagc agtttgtgga tggagtgggt ggcgaaaacg
    12241 gtaggaccgt tgtgatcgcc aggttcgccg cgcatggcat agtgatcggc atagatcggg
    12301 ttgaaacgtg catagccgta ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc
    12361 agcagatcct gcgcttcttt agcgttataa cacacgccgt cataggtgca gttggttacc
    12421 acgcagtaag acggtttttg cccggctttg tctttggtca gcgggctttc actgattttc
    12481 ttttgcaagg tttcaggttg catttcctgc ggatagattg gcccgataat gccgtagcgg
    12541 ttgcggcttg gcaccatata gaccggtttc gcgcctgtca gcatcaaacc ttgttcgatg
    12601 gatttatggc agttacggtc aacgaccacg acatcgttat cggtcatgca agcctgcatg
    12661 atggtgcggt tagagccgga agtaccgacg actaccgacc aggagcgatc ggcaccaaat
    12721 acgcgtgcgg catatttttc gctttcgcca aatgcgccag tatggtcaag caaagaaccg
    12781 agggaagttc gttcgatgcc catgtcggta cggaacagat tttcaccata gtagtcatgg
    12841 tagaaacgtc cggcgggtgt tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc
    12901 caggaatatt catggatgtc actatatttc atcagcgcgc tgaacagtgg cggcaacagc
    12961 tgctggcggt agcgggtcat cgcggcaacg gcgcgtccgg cgataaagtc ggcggtatct
    13021 tccagaatcc aggcgaattc atcgacaagc tccagcagat cccggtccat tgcggcgagg
    13081 gctttttccc gatcgcccaa caggaagacc ggcacgtttt gttggcgctc atgaagctta
    13141 ccgatcaatt gtctgacgtt ttgatgttcg tccggatgct ccatttgata gctgaacatc
    13201 aggcagtcaa tggcttcgtt tgaagagaga atggcaaaac catcatcaaa ggaggtggat
    13261 ttaatcacgg taacattttg ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg
    13321 acccaggtgt cttgatggag aaactcgctt tcaacaatta atactttcat cattgcttac
    13381 ccggttatga aggaatcttc gttgcgggca agtatcttcc ggccttttcg ggctgtaaag
    13441 cgcgtgaaaa acaaacattt
//
LOCUS       DAZPFP010000097        13248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPFP010000097 DAZPFP010000000
VERSION     DAZPFP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.097"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUR4_002624"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUR4_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUR4_002625"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUR4_002625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1456158.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUR4_002626"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUR4_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUR4_002627"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUR4_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1456159.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUR4_002628"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUR4_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1456160.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUR4_002629"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUR4_002629"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1456161.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUR4_002630"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUR4_002630"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1456162.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUR4_002631"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUR4_002631"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1456163.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUR4_002632"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUR4_002632"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1456164.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACHUR4_002633"
     CDS             9656..10102
                     /locus_tag="ACHUR4_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1456165.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACHUR4_002634"
     CDS             10113..11564
                     /locus_tag="ACHUR4_002634"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1456166.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACHUR4_002635"
     CDS             11554..12624
                     /locus_tag="ACHUR4_002635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1456167.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACHUR4_002636"
     CDS             12624..>13248
                     /locus_tag="ACHUR4_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR1456168.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3458 c         3490 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZPFP010000098        13194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPFP010000098 DAZPFP010000000
VERSION     DAZPFP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.098"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHUR4_002637"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHUR4_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1456169.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHUR4_002638"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHUR4_002638"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1456170.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUR4_002639"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUR4_002639"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1456171.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUR4_002640"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUR4_002640"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1456172.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUR4_002641"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUR4_002641"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1456173.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUR4_002642"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUR4_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1456174.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUR4_002643"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUR4_002643"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1456175.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUR4_002644"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUR4_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1456176.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUR4_002645"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUR4_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1456177.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUR4_002646"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUR4_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1456178.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHUR4_002647"
     CDS             complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHUR4_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1456179.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHUR4_002648"
     CDS             complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHUR4_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1456180.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHUR4_002649"
     CDS             complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHUR4_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1456181.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12441..12881)
                     /locus_tag="ACHUR4_002650"
     CDS             complement(12441..12881)
                     /locus_tag="ACHUR4_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1456182.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHUR4_002651"
     CDS             complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHUR4_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1456183.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT         3359 a         3369 c         3359 g         3107 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttcttctggc tccgcattgg cctgttgctg ttccagcttg cgtgctttgg
     9421 cgcgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct tctggttccg
     9481 cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct gcttcaacgg
     9541 cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt tctgcctgtt
     9601 tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg ttatccgggc
     9661 gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct ttcacccgcg
     9721 ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct gcgctcttat
     9781 gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg
     9841 ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca gctttttcct
     9901 gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt
     9961 caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc
    10021 agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg ctacaccgga
    10081 tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc aacagacagt
    10141 tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt agcgggccac
    10201 ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat aaatgacgca
    10261 ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg
    10321 ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact gcataagcag
    10381 tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt ggaacctgct
    10441 tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg gttggaatca
    10501 cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg
    10561 gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc gcaagaatgc
    10621 gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt
    10681 acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca ccaccctgca
    10741 atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac tgatgtatgc
    10801 gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg cgcgggatcc
    10861 agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct gaaggatgag
    10921 ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga acaggcagca
    10981 ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg acgctaacgc
    11041 acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc tgcgccagta
    11101 gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga tggatgccgc
    11161 cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc atggtgttcc
    11221 acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc aggtgtttct
    11281 gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa attgcagccc
    11341 gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat ggcgtctacc
    11401 ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat aaccgccacc
    11461 atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc gacattaagc
    11521 aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt gattttttca
    11581 ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca ctgaccacac
    11641 tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa
    11701 cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct cacggcggca
    11761 acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc ataaaggcca
    11821 gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt gccgggacat
    11881 cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc gagaaaccga
    11941 cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga ttgatattca
    12001 gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa atccccagca
    12061 gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc acgaccacag
    12121 caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg atcaaaatcc
    12181 acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt gttgccagcc
    12241 ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag agaccgagaa
    12301 acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac aggtagtcag
    12361 tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga gcaggcgaca
    12421 acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg cgacgagcgt
    12481 ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg gcgaacggct
    12541 atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac ggaaataagc
    12601 agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc gatggtcaac
    12661 cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca taagatttgt
    12721 gtcgtcagtg cttgtccgcc gagcgtttga aatgtttgcg gagaagataa cgcagcggta
    12781 tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc caacgggcca
    12841 agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc agaatagcga
    12901 cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa ccgatcgcta
    12961 ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt caaacaaacg
    13021 cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat tgatcagttc
    13081 ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac tttcgagtga
    13141 actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc cata
//
LOCUS       DAZPFP010000099        13127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPFP010000099 DAZPFP010000000
VERSION     DAZPFP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.099"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(109..429)
                     /locus_tag="ACHUR4_002652"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="ACHUR4_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="ACHUR4_002653"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="ACHUR4_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUR4_002654"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUR4_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1456184.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUR4_002655"
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUR4_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1456185.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUR4_002656"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUR4_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1456186.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUR4_002657"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUR4_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1456187.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUR4_002658"
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUR4_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR1456188.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUR4_002659"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUR4_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="ACHUR4_002660"
     CDS             10723..11613
                     /locus_tag="ACHUR4_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1456189.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="ACHUR4_002661"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="ACHUR4_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1456190.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12603..12679)
                     /locus_tag="ACHUR4_002662"
     tRNA            complement(12603..12679)
                     /locus_tag="ACHUR4_002662"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13127
                     /locus_tag="ACHUR4_002663"
     CDS             12922..>13127
                     /locus_tag="ACHUR4_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR1456191.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3221 a         3405 c         3330 g         3171 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DAZPFP010000100        13096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPFP010000100 DAZPFP010000000
VERSION     DAZPFP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.100"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            199..849
                     /gene="csgD"
                     /locus_tag="ACHUR4_002664"
     CDS             199..849
                     /gene="csgD"
                     /locus_tag="ACHUR4_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1456192.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            854..1243
                     /gene="csgE"
                     /locus_tag="ACHUR4_002665"
     CDS             854..1243
                     /gene="csgE"
                     /locus_tag="ACHUR4_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1456193.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1268..1684
                     /gene="csgF"
                     /locus_tag="ACHUR4_002666"
     CDS             1268..1684
                     /gene="csgF"
                     /locus_tag="ACHUR4_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1456194.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1711..2486
                     /locus_tag="ACHUR4_002667"
                     /pseudo
     CDS             1711..2486
                     /locus_tag="ACHUR4_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2550..3041)
                     /locus_tag="ACHUR4_002668"
     CDS             complement(2550..3041)
                     /locus_tag="ACHUR4_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1456195.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3143..3697)
                     /gene="ycdY"
                     /locus_tag="ACHUR4_002669"
     CDS             complement(3143..3697)
                     /gene="ycdY"
                     /locus_tag="ACHUR4_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1456196.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3721..4458)
                     /gene="ycdX"
                     /locus_tag="ACHUR4_002670"
     CDS             complement(3721..4458)
                     /gene="ycdX"
                     /locus_tag="ACHUR4_002670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1456197.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4513..5451)
                     /gene="ghrA"
                     /locus_tag="ACHUR4_002671"
                     /pseudo
     CDS             complement(4513..5451)
                     /gene="ghrA"
                     /locus_tag="ACHUR4_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5686..5773
                     /locus_tag="ACHUR4_002672"
     tRNA            5686..5773
                     /locus_tag="ACHUR4_002672"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5720..5722,aa:Ser,seq:gga)
     gene            complement(5923..6204)
                     /locus_tag="ACHUR4_002673"
                     /pseudo
     CDS             complement(5923..6204)
                     /locus_tag="ACHUR4_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6486..7490)
                     /locus_tag="ACHUR4_002674"
     CDS             complement(6486..7490)
                     /locus_tag="ACHUR4_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR1456198.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7562..8122)
                     /locus_tag="ACHUR4_002675"
                     /pseudo
     CDS             complement(7562..8122)
                     /locus_tag="ACHUR4_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8207..8404)
                     /locus_tag="ACHUR4_002676"
     CDS             complement(8207..8404)
                     /locus_tag="ACHUR4_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR1456199.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8416..8907)
                     /locus_tag="ACHUR4_002677"
     CDS             complement(8416..8907)
                     /locus_tag="ACHUR4_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR1456200.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8904..9278)
                     /locus_tag="ACHUR4_002678"
     CDS             complement(8904..9278)
                     /locus_tag="ACHUR4_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR1456201.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9325..9699)
                     /locus_tag="ACHUR4_002679"
     CDS             complement(9325..9699)
                     /locus_tag="ACHUR4_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR1456202.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9862..10083)
                     /locus_tag="ACHUR4_002680"
     CDS             complement(9862..10083)
                     /locus_tag="ACHUR4_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR1456203.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10146..10622)
                     /locus_tag="ACHUR4_002681"
     CDS             complement(10146..10622)
                     /locus_tag="ACHUR4_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR1456204.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10638..11111)
                     /locus_tag="ACHUR4_002682"
     CDS             complement(10638..11111)
                     /locus_tag="ACHUR4_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1456205.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11453..12274)
                     /locus_tag="ACHUR4_002683"
     CDS             complement(11453..12274)
                     /locus_tag="ACHUR4_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1456206.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12374..12547)
                     /locus_tag="ACHUR4_002684"
     CDS             complement(12374..12547)
                     /locus_tag="ACHUR4_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1456207.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(12667..>13096)
                     /locus_tag="ACHUR4_002685"
     CDS             complement(12667..>13096)
                     /locus_tag="ACHUR4_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456208.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3310 a         3235 c         3218 g         3333 t
ORIGIN      
        1 ttgttacatg tttaacactt gatttaagat ttgtaatggc tagattgaaa tcagatgtaa
       61 tccattagtt ttatatttta cccatttagg gctgatttat tactacacac agcagtgcaa
      121 catctgtcag tacttctggt gcttctattt tagaggcagc tgtcaggtgt gcgatcaata
      181 aaaaagtggg gtttcatcat gtttaatgaa gtccatagta ttcatggtca tacattattg
      241 ttgatcacta aaccttcttt gcaggcgaca gctctcttgc agcaccttaa acaatcgcta
      301 gcaattacag gaaaattaca taatattcaa cgttctctgg acgatatctc ttcaggctct
      361 attattcttc tggatatgat ggaagcggat aaaaagctta tccattattg gcaggatact
      421 ttgagcagga aaaacaacaa tatcaaaata ttgttgctaa atacgcctga agattacccg
      481 taccgcgaca ttgaaaactg gcctcatatc aacggcgttt tttatgccat ggaggatcaa
      541 gaacgtgttg tcaatgggtt gcaaggcgtc ctgcgtggcg aatgctactt tacgcaaaag
      601 cttgccagtt acctgattac acattctggt aactatcgtt ataacagcac ggaatcagcc
      661 ctccttactc atcgggaaaa agagatcctg aataagctgc gtatcggcgc gtctaataac
      721 gagatcgctc gttcgttgtt catcagcgaa aatacggtta aaacgcatct ttataatctt
      781 ttcaagaaga tagccgtaaa aaaccggaca caagcggttt cctgggcaaa cgataacctc
      841 aggcgataaa gccatgaaac gttatttacg ctggattgtg gcggcagaat ttctgttcgc
      901 cgcagggaat cttcacgccg ttgaggtaga agtcccggga ttgttaactg accatactgt
      961 ttcatctatt ggccatgatt tttaccgagc ctttagtgat aaatgggaaa gtgactatac
     1021 gggtaactta acgattaatg aaaggcccag tgcacgatgg ggaagctgga tcacaataac
     1081 ggtcaatcag gatgttattt tccagacttt tttattcccg ttgaaaagag actccgagaa
     1141 aactgtcgtc tttgcactga ttcaaactga agaagcacta aatcgtcgcc agataaatca
     1201 ggcgttatta agtacggacg atttggcgca tgatgaattc taaataaaaa attgttcgga
     1261 ggctgcaatg cgtgtcaaac atgcagtagt tctactcatg cttatttcgc cattaagttg
     1321 ggctggaacc atgactttcc agttccgtaa tccaaacttt ggtggtaacc caaataatgg
     1381 cgctttttta ttaaatagcg ctcaggccca aaactcttat aaagatccga gctataacga
     1441 tgactttggt attgaaacac cctcagcgtt agataacttt actcaggcca tccagtcaca
     1501 aattttaggt gggctactgt cgaatattaa taccggtaaa ccgggccgca tggtgaccaa
     1561 cgattatatt gttgatattg ctaaccgcga tggtcaattg cagttgaacg tgacagatcg
     1621 taaaaccgga caaacctcga ccatccaggt ttcgggttta caaaataact caaccgattt
     1681 ttaagcccca gcttcataag gaaaataatc atgcagcgct tatttctttt ggttgccgtc
     1741 atgttactga gcggatgctt aaccgccccg cctaaagaag ccgccagacc gacattaatg
     1801 cctcgtgctc agagctacaa agatttgacc catctgccag cgccgacggg taagatcttt
     1861 gtttcggtat acaacattca ggacgaaacc gggcaattta aaccctaccc ggcaagtaac
     1921 ttctccactg ctgttccgca aagcgccagg gcttacaaaa cctgcttaac gagcgcaagg
     1981 ttattcgtgc ggcacaagaa aacggcacgg ttgccattaa taaccgaatc ccgctgcaat
     2041 ctttaacggc ggcaaatatt atggttgaag gttcgattat cggttatgaa agcaacgtta
     2101 aatcgggcgg tgttggggca agatattttg ggatcggtgc agacacgcaa taccagctcg
     2161 atcagattgc cgtgaacctg cgtgtcgtca atgtgagtac cggtgagatc ctctcctcgg
     2221 tgaacaccag taagacgata ctttcctatg aagttcaggc cggggttttc cgctttattg
     2281 actaccagcg cttgctggaa ggggaagtgg gttacacctc aaacgaaccc gttatgctgt
     2341 gtctgatgtc ggctatcgaa acaggggtca ttttcctgat taatgatggt atcgaccgtg
     2401 gtctgtggga tttgtaaaat aaagcagaac ggcagaatga cattctggtg aaataccgcc
     2461 atatgtcggt tccaccggaa tcctgacaga taaaaatgcc ggaattaata atccggcatt
     2521 tactgtttaa gcgaaagcgt gagtgcccct cacgctttat ttttgatttc ctgctcacgg
     2581 tgagccgtct ttgcactacg cgccgccagc cacaggccgc tgtttttcat tgcgtaacca
     2641 aataccagcc caagcgccag cgaaggtaac accagtttcc agtcaccctg cccggcaaac
     2701 gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa gcagcagctg tttggcctga
     2761 atacacatca gaaacgcgac aataccggtt atgacataac cgagaatttc cagatgtggt
     2821 gccagtgcac taccgtaaat aatgaccatc gcccacacca cgccactaag cagggttgca
     2881 gcggagatcg ccagcccttt cagcccacct tgcgggcaag caaagtaggc cgtacagccc
     2941 aggaagcccg cccagcttag taagcccagg gaaacagcca cccatcccca gataccggag
     3001 agaatgcctg ttgtgattgc aatagagaga agtatgttca tgccgcgcat gatagcagaa
     3061 aacgcggcca tgaatgagat cgagcacaca ttttatgcaa caacaaatta tctgttgcag
     3121 ttatgagtga cgatgatcac atttactctt cagaatcttc ttccagctcg tcccacattg
     3181 cactaatggc atcacgggtt agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg
     3241 cctccacttt gccgaggaac gcaccacacc agggtaacag atactcactg aacagtgttt
     3301 ccagtgcttc gctctcatct tccgttgact gatcttccag ccaggaagcc gcaagcagca
     3361 atgtgccgat gtgatcggct ggcgtatccg ccagtagcat ccctcgttcg gaaagaaaag
     3421 cgcgcacttc cgcttccgtt gcgtcctcaa cccatgcgct acgatatggc ggcacggcac
     3481 attcatcgcc gataaacaac gcattgtaat cggcagagac ttgcgtcata tcacagcttt
     3541 tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca gttcgcagcc agtttcccct
     3601 cacgaatcag agtaaacagc ggcaccagta aaggatcttg cggttggcgg taatagagcg
     3661 aacccagcac acgacagagg atagaaaact cgttcattaa tatatttcca ttactagtaa
     3721 ttaaaggtct gcaaattccg caatcggtgc cataccgcga gattcaagga agttcagtaa
     3781 gcggcacgga gaaacattca aaatgcgctc tagcggaaaa tctaccgcgt cgaggatttt
     3841 aagacactct tcaaattccc ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac
     3901 ccaacctcca gcatcacgta ccgctgcagc taccgcacga cagttgtctt cactgccctt
     3961 acgtgagtgt aaaaatgagg aattattgat ttccagcgcc acctgatgtt tcgcggctgc
     4021 ttcagcaacg gctttaacat ctatttcata tttcggattt ccgggatggc ttattatatg
     4081 cacattgccg ctggcgatag tggagatcat cgcttgtgta ttggtcgctt tgtcatgtgg
     4141 cgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc agcgagtcaa acattttacc
     4201 gctgcagtca atttcaccat caacattttt aatgttagct tcaatgccgc gcaggatccc
     4261 taccccatca accactcgcg gccagatacg catgttaatg aagtgccagt gatgcggcgc
     4321 atcttccata tccgggccat gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc
     4381 ctgggcaatg taatcactca atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg
     4441 aaggtcgacg ggatacataa ctctctcctg tattcaattc ccgccaagga tagcaggaat
     4501 cctgatgctt tattagtagc cacgtgcgcg gtcgacttgc ccgcagaccc tctccccttt
     4561 ttcgagctgg gcaatagtgc gagaaatgta ctccacagct tcagcgggac gggtaatcgc
     4621 ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag agcggacttt caggcggtaa
     4681 gggttcacga ttaaaaacat ccagcattgc gcctttaact ttcccgctat ccagcgccgc
     4741 gagcaggtca tcttccacaa catgaacacc acgcgccagg ttgaggagat acgcgccatc
     4801 aggtaatttt tcgagtaatt gttgattaat aatgccgacg gtttcagggg tattcggtaa
     4861 caaattaatc aatacccgac attggctcag aaatgcagac agttcttccc gtccggcaaa
     4921 gctttgcacg ccaggccacg atttacgggt tcgactccag caacgcagcg gaaagcgcta
     4981 ggtttgcaga ctctgagcaa ctttacagcc cagtacgcct gcgcccaaaa tgccgatggt
     5041 aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc gaactatttt gctggatgcg
     5101 ataatcgtca aaacgtcgaa accaatgcag cacctgactg acagcatatt cctgcatttg
     5161 ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca gaagggttca gcatttcagg
     5221 gtgtgcctgt agcttgctca aaatagaatc aacaccggcc ccgagtgcga acaccgcttt
     5281 aagatcgcgc cctgccagca tttcaacagg aggatgccag actaaaacat aatcagcaga
     5341 gtcattatct ccgcttttcc atgccctgac tcttgcctga ggaatagctt tgcgcagtgc
     5401 ctcaatccac cattgggtat cgaacgttgg gtgataaaag atgatatcca tactgactcc
     5461 cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat gatctacaaa ggccaccagc
     5521 ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg cttattaaaa ccacacattg
     5581 attgaatgtt tgaataaacg cgcgattttt tcaaaaagtt tgttgacctc aggtcatgat
     5641 ttccctaaat tagcgcccgt tccagcaaga caggaacgac aatttggtga ggtgtccgag
     5701 tggctgaagg agcacgcctg gaaagtgtgt atacggcaac gtatcggggg ttcgaatccc
     5761 cccctcaccg ccagattata agtgcatgtt attgtttaac atgcgctttt ttatttttcc
     5821 ttccagacag tatgccatcg ggtatgccat ctgaaaccgg tttatatgcc ccgcgatcat
     5881 atcttatcgg acaatttccg atctttgagc atactggcaa agctatactg ccagcatatc
     5941 aggatagtgt tttgcgataa tatcattcag tctgtcaacc agttctggtt ttctgaacgt
     6001 gatatgcgcg gagcccttct taaagtatct gatgctgaac atctcatctt catagctctc
     6061 tttaccctga acagactgaa tgtgatcatc cagacgaagg gtgatgtttc cccggttgtc
     6121 ggggattggt ttaccgctga acaggtgcaa cattctttcc agatcggcga gtcggtcagc
     6181 ctgttgtccg gtgataagat gaaatctatg gtcacccccg tttttgcaac actgattttt
     6241 gattaatggt tggcttgctt aaatctatcc ggcgtctctg tgggcaagga tgcccgcgcc
     6301 ccgatgagtt ccgcacctga taatcctaaa aatcccctcg gcttcagtga gccatttcat
     6361 ctgttaggtt tttaatcagg tcgatttgcc gttcatgtca tcaataatca gtcttcgcaa
     6421 aaccagaaag gtaacttgta tcttattgtt taaaataaac taattgagtt atatcagcat
     6481 ctatatcagg cctgatattc ttttttctga gtagtgattg cccaggcaat tcgagccagt
     6541 ttatttgcca gagcgcaggt aaccacattc gaatgcttct tgttgagttg ctcccgaacc
     6601 cactcagcaa gttttccatg cttgcgttca agtcgcatta tgaatgagcg ggcacactga
     6661 accagtaacc ggcgtaaatt tttatcacct cgcttactga tacccaacag agtcgatttt
     6721 cctccagtgc tatactgccg gggaacaagt cctgtggaag ctgcaaaatc cctgctgcat
     6781 gagaactgtt tcccgtcacc aagttgcgac gatagtaagc tggcagtaat cggaccaaca
     6841 ccaggaattg tcataatgcg ctgagcggta tcatcagcgt tgattgactg acttaattct
     6901 gattccagct ctgctatttg ctcaacaagg taaaagtagt gggtatgcaa cctcaccaaa
     6961 aggcggctca gatattcggg gatttcgtgt tcagccaaca cgagagacaa ccgttttatt
     7021 acagcatcac ctatcggcag gctgattcca aattccagca agaaaccgtg gatctggttg
     7081 gttgttttca ccttatctct gatgagcgat tctctgaccc ggtgtaacgc cctcatggcc
     7141 tgctgggtct cagttcttgg ctggacaaaa cgcattgatg gtcgtgaagc cgcttcgcat
     7201 atggcttctg catcaacaaa atcattctta ttacttttga caaacgggcg aacgaactgt
     7261 ggagaaatca gcttagtttc atgcccaaaa tcagagatac agcgagccat aaagtgtgca
     7321 cccgcacagg cttccattac aacaactgag ggcggacatg tcgccagaaa ctgcatcagt
     7381 ttagtacggg taaatttttt acgcagaaga gccttaccag acttgtcttg acaatgaatg
     7441 tggaaggagt gctttccgag atcgatacca ataagtgtga tgttctgcat gatggtctct
     7501 ccgttaaagg gatttacctt aacagcgtac tgctgttaag gtgggggtga ccatatcatt
     7561 atccccaccg gtcccatctc accagattgt tgacgataat tttactgcca aatttacagg
     7621 ggcaattggt tttgtaattc cagttcagcc ctctgaagac gttgatcacc cctcgctcaa
     7681 acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc gcttcgctga
     7741 tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggcc tgcgtatcca
     7801 ttaatgacag catcccggaa cgttgcatca ggtcacgcca gatttcgcga tcaagattcc
     7861 gggtaatggc tttcattgct gtttcgggtt tctccatcag ccagcagccg cagcgaaaat
     7921 cctgtttcat ggcccagtcc ggggcagttt tacctccgat gctttccgtc agcaccgaga
     7981 tttcagccag ttgtcggata agtgtgtcga tttgcaacag tgtactcttt cgtacagcca
     8041 gaatgtggcg aatgctggtt gagcagataa cgtccgtatg cccgttgacc acttccggac
     8101 gggagattgt gatatctgac ataatcgata ttcctttttg cagataaatg aaaacgccag
     8161 ccctgtgcag gctggcgtca gaggaatgaa gtctggtgag tgagcttcac cggttctggt
     8221 gcagaagctg cagatgacgc agggtacgga catcctcccg ggcctgttcg atacagggca
     8281 acagtgctgc tgaatccgtt ttatcctcat cgttgtcaaa gataattccc gatccgcagt
     8341 cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt
     8401 tcatgttgtt cctggttacc agtcgaaagt gggtaaaccc ggcaggcatt cagcgctggc
     8461 atccagatga atgagactga cgccataacg ccggatgagt gtgacgacca gacgacggaa
     8521 cgtttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg catcggtacg
     8581 taacagatat ccgctacctg taaaatgaat ccattctgat tcaccgaagt cactggtctg
     8641 gtgtgacagc gagtacaacc aggcgttgtc cgtttccgtg atatgtgcgg tactgcagcg
     8701 tatgccggta agggtagcaa atggtggcgt gcattctgct gaggtctggt cagattcatc
     8761 aacaacacga agggagtagc cgttatcgca gaccatgtta atcagttcag cgagctcaat
     8821 accatcgacg tcaacgacaa tgcgccctat gttcagtgcc tgtacgttaa cgctgtcggc
     8881 ttccagtgtc agggccagtt tcatcatttc gcctccggat acttacccag ggtaatgtta
     8941 tttaccgttc tgtaattgtc gcgggtcatc aggccggtcg cccggcgagc ccggaggata
     9001 tcaatactgt ttattaactg agagcgggga caggtgctga atcccggctg gtcggtacgc
     9061 accagcgcgt atttttccac gagaaaatta actgcatcac acagtgaaat gcctgcctca
     9121 atatgctgct caatcacacg ttcatcgaca aacggtgtgt cattcagtgt gaggccataa
     9181 tgctggtcca gcagtcggat gagcagtgtc tgccagatgg tgacgggaga cgggcagcga
     9241 gatacctccc gtacatgtgt gtcggataat gttttcatct taagttctct gtaactggta
     9301 gtgctaaagg tgagagcttc ggggttatga ggtggttgcg ggtgctggtg tcggataaac
     9361 agccagataa acgtaaccac aggagccgag ggtgtcggct tcgcaggtca gcccttttgc
     9421 atacagcgtg acggtatgct gatatcgggg attcagttca ccgctggtga gcatgagttc
     9481 cagttgtttc atcagcagcg gaaaggcctg gtccagatgg tatgcatctg cgtcgctgaa
     9541 gcggcctctg ataccggcgc ggtcggcaag gtaatacagg cggttaccct cttgcaccag
     9601 acgagcgcca aaacagggcc tcactgtgca gggaagcccc caccaggggc ggtcgtgatt
     9661 gtcatcggga tgagttatcc cggagagttt gtctgacacg ataaaatcct ctctgaaaag
     9721 tggttaagaa tggtttaacg atgacaggtc agccggcgcg ctgaccggtt tcggtgaaga
     9781 tgtatgcatt gatgagaatg gcctcatcca cagcggcgtc tgaggtcatc tggtcgtact
     9841 cgtttcccgg gtgacggaac atcagttcag ggtgacactc atcagccgga catacgggcc
     9901 aaaactgtcc ttacggcgtt ctgcaaacac ggcaagaacg ccgggaatat cctgcacttc
     9961 acgaccagta tacgcttcag cactgccgtg ccagcgatac ttaccggtac agaacggaaa
    10021 aagacgggat gcaggatgct gttggtgaat acgcatggct tcaccacggg tgatgattct
    10081 catgatgtga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga tgtggttgtg
    10141 acgggttaaa gcagaccatg ttcggcaaag gagaaaacct ggttgccacc gactatcaga
    10201 tggtccggca cccggatatc caccaggccc agtgcctgta ccagacgttc ggtgataagc
    10261 cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgcgccag caccacggca
    10321 gcggcattgt ggtacagggc gcgtttaatc acttcccggg gatgaacttc cgtacggttg
    10381 atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttgtt cagatacagc
    10441 acccggaact cttcccgctc cagtcccgcc atgttcagaa tcagccattc ccgtgccgca
    10501 cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt ttgcagggcc
    10561 cgcagaatga gactgcgctc gccgggcgtc atgtttccgg gcagaaagga aagctgctgc
    10621 attgtccctg cctccattca gtcgatgatg cgcataatgg cgctgcattc cggatgctgc
    10681 agggcgtaat cccgcagccg gtaataatgg accgtcatgg cataacactc cgtacgacag
    10741 gcatgatgac tgtacgtcat cagacaggca gcaatgccgg cggcttccgg gctcatttca
    10801 gcgcggttac cgttcatggc attgaacagt acccatgttt cgtcatcatc gtcatccggt
    10861 tcgggtgcca taaatgcccc gccgttgttc agggtgtaca gattccagat accaccgcag
    10921 tagtcttcac acagacggtc catccagccg aagacacggg gttccagggt gacccactgt
    10981 ggaatgaggc caaaatgctg cggccagaag ctgatgcgct gttcatcagg gacgggagtg
    11041 gcaaccagtt gttgcaggcc agtctccggt gcacaggccg aagcggtggt gttctgagac
    11101 aatgttttca tgtatgaaat ccttgtaatg tgaataaatg agagggagta aaggtcggga
    11161 gctggcaaca tcacgttcat cactgaccgt gatgacccgg taatatcagg gatgaatgat
    11221 attcggctga aattccggac agccgtacgc gtaaccgttc cggtcacaga cggatggcca
    11281 gatgccattg cctgtatact cagtctgcac agactgattt ctccgggacg gtaaagcaga
    11341 cgggtacacc aggcgcacag gtcatcatca gaaatggaac gacaccccgg cagcgcctcc
    11401 ggggcgtcgg gtgattctgg tgcctgaatc acgccttcag ggacatgctg cctcagcgca
    11461 ggctctccag cagcgtttct gccattaccc acaatgcgcg gttgagctta atatcggtgt
    11521 cgatgttgtg aatggcacgg gtgtggatac gttttccttt tgcactgcgg ccggaaatcc
    11581 cgcctttcag catattctcc tggatggtct gatatgcgct ccacaggtct ttaccgtaat
    11641 cctccctgcg tcgtggtgtc agaatgtcag cggtggtgac tggctgatgt tcgtcaccat
    11701 aacggtaagt cagtgccgcc tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg
    11761 actgcatggc atcacgcttc tcctctatcc ggtcaaacac gcccaccacc tcgtaagccc
    11821 cttcaataac tttctccacc acatttcccc ggtgtggaac acgcaattct cccagagact
    11881 gaccgcagac cagtccattg gtacaaatgg cacgaaaata tccgggtaac atctggtagc
    11941 tggatgaacc gtcatgagag ttgagcagaa taatttcagg gacatgctga ccggttatct
    12001 gtccggcccg ccgcagacgc agcatgtgtt ttgtgtattc ccggcgaccc gggtcacgca
    12061 cacgtgtctg gcaggcgaag aatggctgaa agccttcccg ctgcaggctt tccagtacgg
    12121 tgatggtggg agtgtacgca tagcgtttac tgcgggaagt atgccggtct tcaccgaaaa
    12181 tactgggaac atagtgcatc agttcttcgt gtgtcagcgg acggtcacgg cgtatctggt
    12241 ttgcagcata accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga
    12301 tttactggtg taataaatga aaaagccacg tctcccggag aagacgcggc ctgatggacg
    12361 aaattaatga ggtttattgt ctgagaagac cgtcactggc gaaccttccg gcgtcagtct
    12421 ctgcgttgcg gataacccga cggaaatgag ttccctggtc atcgtcgata aacacattgc
    12481 ggtaagctgt tgtgatgaca acagcctgct cgcgtgagaa agggacttcg ggcaataaac
    12541 gtgacataca accagacctg tatttggttc cctgaaaata taagtccctg tcatcatggc
    12601 agagccgtat ttacttttga tatcaataaa ttaataacgc gaagatgaac cgcataactt
    12661 acgcctttaa tttgttttgt ttacgtagtc cctgaccagg tacgcgaatt cgtgaacgcc
    12721 agtttcacca aaccaaatca ggtaaccagg gtcgatgttg taatagtaac caacgacttt
    12781 atgcgttttt gggttataga gtgttacgat atagtcccag ccgcaggcgt gggctttaca
    12841 accgccacct tctacccagg tcgttccgtt aatgtttaca ggatacgcac caccatcagg
    12901 accatccata tagccgtctt ccccaaacat tccgtttaac ccaacagggt ttaatgcttt
    12961 gtcgatagca gctctgtagg tagcgtcacc ttcgtataac tcgtacgttg ttggacatgc
    13021 accaggctgg tcagcccggg cttttgagtc gcatttaagt gtctgattta cagtggcggc
    13081 gtttgtgtca ggactt
//
LOCUS       DAZPFP010000101        13059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPFP010000101 DAZPFP010000000
VERSION     DAZPFP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.101"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            298..879
                     /gene="mdaB"
                     /locus_tag="ACHUR4_002686"
     CDS             298..879
                     /gene="mdaB"
                     /locus_tag="ACHUR4_002686"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1456209.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            910..1224
                     /locus_tag="ACHUR4_002687"
     CDS             910..1224
                     /locus_tag="ACHUR4_002687"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1456210.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1272..3164)
                     /gene="parE"
                     /locus_tag="ACHUR4_002688"
     CDS             complement(1272..3164)
                     /gene="parE"
                     /locus_tag="ACHUR4_002688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1456211.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3193..3774)
                     /gene="yqiA"
                     /locus_tag="ACHUR4_002689"
     CDS             complement(3193..3774)
                     /gene="yqiA"
                     /locus_tag="ACHUR4_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1456212.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3774..4601)
                     /gene="cpdA"
                     /locus_tag="ACHUR4_002690"
     CDS             complement(3774..4601)
                     /gene="cpdA"
                     /locus_tag="ACHUR4_002690"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1456213.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4626..5048)
                     /locus_tag="ACHUR4_002691"
     CDS             complement(4626..5048)
                     /locus_tag="ACHUR4_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1456214.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5049..5678)
                     /gene="nudF"
                     /locus_tag="ACHUR4_002692"
     CDS             complement(5049..5678)
                     /gene="nudF"
                     /locus_tag="ACHUR4_002692"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1456215.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5883..7364
                     /gene="tolC"
                     /locus_tag="ACHUR4_002693"
     CDS             5883..7364
                     /gene="tolC"
                     /locus_tag="ACHUR4_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1456216.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7512..8183
                     /locus_tag="ACHUR4_002694"
     CDS             7512..8183
                     /locus_tag="ACHUR4_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1456217.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8189..9349
                     /locus_tag="ACHUR4_002695"
     CDS             8189..9349
                     /locus_tag="ACHUR4_002695"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1456218.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9387..10175)
                     /gene="ygiD"
                     /locus_tag="ACHUR4_002696"
     CDS             complement(9387..10175)
                     /gene="ygiD"
                     /locus_tag="ACHUR4_002696"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1456219.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10318..11091
                     /gene="zupT"
                     /locus_tag="ACHUR4_002697"
     CDS             10318..11091
                     /gene="zupT"
                     /locus_tag="ACHUR4_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1456220.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11149..11319)
                     /gene="yqiD"
                     /locus_tag="ACHUR4_002698"
     CDS             complement(11149..11319)
                     /gene="yqiD"
                     /locus_tag="ACHUR4_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1456221.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11580..12233)
                     /gene="ribB"
                     /locus_tag="ACHUR4_002699"
     CDS             complement(11580..12233)
                     /gene="ribB"
                     /locus_tag="ACHUR4_002699"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1456222.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12337..12485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12607..12897
                     /gene="ubiK"
                     /locus_tag="ACHUR4_002700"
     CDS             12607..12897
                     /gene="ubiK"
                     /locus_tag="ACHUR4_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1456223.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3316 a         3485 c         3167 g         3091 t
ORIGIN      
        1 tccatttata attatcaaca tgacacgttg atattttcat cttttactta ttcaaagcca
       61 tattttcaat ccccaaaaaa accgaaccaa acgtgtaaat tattccggtg cgagtaagaa
      121 ttatgaggaa tggctatcag tattgtcatt ttcagaaaat atttatcctg catcggtgag
      181 tcagagctag atcagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc
      241 acagtaccgc tctgtgacgt actatagtcg acaaacgtct caccttgaga ttaaaaaatg
      301 agcaacatcc tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac
      361 gacaccctga ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgtccgcatc
      421 gttcgcgccg acagcgacta caatgtcaaa gcggaagtac aaaactttct ctgggctgat
      481 gtggtgatct ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatat
      541 attgatgatg tattcaccga aggtcacggg actctgtatg ccagcgatgg tcgtacccgc
      601 aaagatccgt cgaaaaaata cggttccggc agcctggtac aggacaaaaa atatatgctt
      661 tctctaacct ggaacgcacc aatggaagcc ttcaccgaaa aagatcagtt cttccacggc
      721 gttggcgttg acggtgtgta tctgccgttc cataaagcaa accaattcct cggtatggaa
      781 ccgctgccga catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact
      841 gaagaatatc gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag
      901 gagttaacca tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt
      961 caggcggtat tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc
     1021 cacggctatg cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg
     1081 gattctatcg tgatgattga gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa
     1141 accccgcaca tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatc
     1201 cgtattctgc agccagggat ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa
     1261 tgttctttcc tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg
     1321 cgatcttccg agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg
     1381 tcttcatcat cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca
     1441 cgcaattgca tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct
     1501 ttcttgcgtt ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa
     1561 acctctttcc cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt
     1621 ttcaccaacg cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga
     1681 ccatcagagt ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg
     1741 tcgctgtcag gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc
     1801 acttcgtcgg aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc
     1861 tgatattcac gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacgagg
     1921 aacagttcgg tacggttaag gccctgcgcg gtacaatcag ccagtttgcc aggcagcgcc
     1981 gggccgctgg tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg
     2041 ctggaaatcg ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg
     2101 atgaaggcat ctttcaccac gcccgaaacg aatgccgcac actgacgcga agagagacgc
     2161 tctttggtct gcccggcaaa ctgcggatcc tgcattttaa ccgacagaac ataggcgcag
     2221 cgatcccaga tatcttccgc cgacagcttc acgccgcgcg gcagaatgtt gcggtattca
     2281 cagaactcac gcatcgcgtc caacaggccc tgacgcagac cattaacatg ggtaccgccc
     2341 tgcatcgttg ggataaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc
     2401 cacagtagcg cccagtccac agcttcagta tcgccagcga aattaccgat aaacggtttt
     2461 tctggcagcg tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga
     2521 tagcaccagc gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg
     2581 cacagtactg ctttggcctt cagcacatgc gtcaggcgtg aaacagaaaa acgcgggctg
     2641 tcaaagaagg tttcatccgg ccagaagtgc acgctggtac cggtattgcg tttaccgcaa
     2701 gtgccgacaa cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc
     2761 tgaccatcgc ggcgcacgtt aacttctacg cgcttcgaca aggcgttaac taccgaaatc
     2821 cccacgccat gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca
     2881 tgcagacggc aaagaatcag ttcaaccgcc ggtactcctt cttccgggtg aatatccacc
     2941 ggcatcccgc gcccatcgtc aatcacttct aacgactggt cagcatgtaa gattacatcc
     3001 acgcgttttg cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc
     3061 aaatggttag ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc
     3121 ccggtgagta cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat
     3181 tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc
     3241 ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcata
     3301 gtacgccacc gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat
     3361 caaatccggc gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga
     3421 ctctagcaca tattgctgcc cggtgtaggg gttctcgttc tgaccgagat agtccgtcag
     3481 caattcaaac gggcgtaccg ccgggtttac caccactgcg ggcagcataa aacattgcga
     3541 caaccaggtg gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc
     3601 atgttccagg acaatggatt ccagcagttc tgccgcgtcg gaaggatacg gcggcaactg
     3661 cggaatgatc atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc
     3721 ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag
     3781 ccttctgaag cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc
     3841 agcgtgccat cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta
     3901 aagttggaac agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga
     3961 ccgttccagt cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc
     4021 ggaaacttcg ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc
     4081 caactacaac ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc
     4141 gcatcggcca gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc
     4201 acgccaaaca cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc
     4261 ttcgccgggg agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg
     4321 ttgcccggca gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga
     4381 taggccgcag aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg
     4441 tgctggtgtg gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct
     4501 aacagggctt cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa aatccttcct
     4561 ctggcctcac cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca
     4621 cgacgctagt aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc
     4681 gctaaaaact ggttaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa
     4741 tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg
     4801 tcatgataca gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc
     4861 tgttctattg tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt
     4921 gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg
     4981 cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa
     5041 cgcttcattt atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa
     5101 gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc
     5161 cggcttacca catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt
     5221 gcggtcgtgg cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg
     5281 cttgccagga aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc
     5341 gcttcgcgac gggcaacatc ttccacactt tcaccctctt caatcatccc ggcaaccatc
     5401 tccagtagcc agggggtttc gctggtgtcg tacgcggcaa tccgaatctg ctcaatcagc
     5461 acaacttcat cacgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa
     5521 atttcccgcc gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa
     5581 agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt
     5641 ttgccaaatg taacgggcag gttgtctggc ttaagcattg ttaatgtcct agcactaata
     5701 gtgaattaaa tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tatgttaaat
     5761 tgaggcacat taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct
     5821 agaatccgca ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc
     5881 aaatgaagaa attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga
     5941 gccaggccga gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc
     6001 gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat
     6061 tactgccaca gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga
     6121 acggcatcaa ctcgaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata
     6181 tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat
     6241 atcagaccga tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga
     6301 atgctattga cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag
     6361 atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc
     6421 gcgcgcagta cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg
     6481 cggtagagca gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg
     6541 tcgaaaactt taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa
     6601 aacgcaacct gtcgctgtta caggcacgct tgagccagga cctggagcgc gagcaaattc
     6661 gccaggcgca ggatggtcac ttaccgactc tggatttaac ggcttctacc gggatttctg
     6721 acacctctta tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgatagca
     6781 atatgggcca gaacaaagtt ggcctgagct tctcgctgcc gatttatcag ggcggaatgg
     6841 ttaactcgca ggtgaaacag gcacagtaca actttgttgg tgccagtgag caactggaaa
     6901 gcgcacatcg tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcttcta
     6961 tcagtagtat taacgcctac aaacaagccg tagtttccgc gcaaagctca ttagacgcga
     7021 tggaagcggg ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcgaccacca
     7081 cgctgtacaa cgccaagcaa gagctggcga atgcgcgtta taactacctg attaatcagc
     7141 tgaatattaa gtcagccctg ggtacgttga acgagcagga tttgctggca ctgaacaatg
     7201 cgctgagcaa accggtttcc actaatccgg aaaacgttgc cccgcaaacg ccggaacaga
     7261 atgctattgc tgatggttat gcgcctgata gcccggcacc cgtcgttcag caaacatccg
     7321 cacgcactac caccagtaac ggtcataacc ctttccgtaa ctgatgacga cgacggggct
     7381 tcggccccgt ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc
     7441 cttctcgctt caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc
     7501 ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga
     7561 gcgcacgcca tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct
     7621 gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa
     7681 acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac
     7741 gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt
     7801 gtcagcaagc accagcccag gctggcatgg caccagaaaa ccaggcgcag gctcagcaat
     7861 ccagcgggag tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg
     7921 gcggcgcggg atttgcacag cagccgctgt tctcctcgaa aaacccggcc agtccggctt
     7981 acggtaaata taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca
     8041 tgaccgtacc gaagacggca atggcaccaa aaccggcgac caccactacc gttacccgtg
     8101 gcggttttgg tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggcacct
     8161 cttctcgttc aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga
     8221 ctggcgtgag aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta
     8281 ctggtgtgaa gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt
     8341 caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga
     8401 gctgatgacc aaattccgca taccaaaaca cacctggagt tttgtgcgtc aatcatggct
     8461 gactcaccag ccatcgcttt attcgcgtct tgatctggcg tgggatggca ctggcgaacc
     8521 taaacttctg gaaaataacg ccgatacgcc aacgtcacta tacgaggcgg cgttctttca
     8581 gtggatctgg ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt
     8641 taacagtctg caagaaaaac tgatcgatcg cttcgttgag ctgcgtgaac agtatggctt
     8701 ccagttgctg catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta
     8761 tttgcaggac tgcgcaaccg aagctgaaat cgccaccgag ttcctctaca tcgacgagat
     8821 aggtttaggc gaaaaaggcc agttcacgga tttacaggat caggtgattt ctaacctgtt
     8881 caagctttat ccgtgggaat ttatgctgcg cgaaatgttc tccaccaagc tggaagatgc
     8941 tggcgtacgc tggctggaac cggcgtggaa gagcattatc tccaacaagg cacttctacc
     9001 gctactgtgg gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga
     9061 tgatcatccg caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa
     9121 cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga
     9181 agggatgatt gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat
     9241 tggtagctgg ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt
     9301 gatcacccag gatatgtctc ggttttatcc acatattttt gttgaataag ccacgatacc
     9361 ggatggcact cgccatccgg taattgttag cctatctgca ccgacagcat acttaaactg
     9421 cccatttcta taccatcgac cggaatggta attggctcct gcccatccca cacacctaac
     9481 acatacaaca acggcagata gtgctctggc gttgggttcg ataacgcgcc accttcatgg
     9541 tcgagataat tcaccagagg atgctgctct accggccctt gccacgtcag attcgctttc
     9601 acatactcat taaacgacgt cgcccacgga tacggtgaac tatcaccgtg ccacttcact
     9661 gtgcgcaggt tatgcaccac gttaccgctg gcgaccaaca ttattccttc atcacgcagc
     9721 gccgcgagtt tgcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc
     9781 aactgcacca tagggatatc ggcgtcagga tacatcttaa tcagcacgcc ccacgagccg
     9841 tggtcaaagc cccaggcttc tttatccagc gtcaccggga tcggcgctaa cagctcaacc
     9901 agacgctgtg ccagcgcagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc
     9961 gggaagccac caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca
    10021 cgggtaaacc agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc
    10081 aacttctgcc agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga
    10141 cctaaaaaca atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc
    10201 attttgatat cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga
    10261 tgatcatcag ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg
    10321 tcagtacctc tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc
    10381 ggcgttctcg gacaaaaacc ctcgaaccgc ttactggcgt tttcgctagg ttttgcggcg
    10441 gggatcatgt tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga
    10501 atgtcgccgg tgttgggtta tggaatgttt atcttcggtc tgcttggcta ctttggcctg
    10561 gaccgcatgt tgccgcatgc gcatccgcag gatttaatgc aaaaatcggt gcagctgttg
    10621 ccaaaatcga tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc
    10681 ccggaaggga ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc
    10741 gcactggccg tcgcgttgca caatatccct gaaggtctgg cagtggcagg cccggtttat
    10801 gcggcaacgg ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa
    10861 attcttggtg gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg
    10921 gcggcaatca tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg
    10981 ccgctcgcca aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg
    11041 tcagtgatgg gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat
    11101 cagaagttgt ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt
    11161 ttcggctgtc tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc
    11221 tctgtcctgg cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac
    11281 cagagcaatc aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa
    11341 caatactaaa catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt
    11401 cgcataataa cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta
    11461 atgaaatttt ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa
    11521 aaaccgaagc cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt
    11581 cagctggctt tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc
    11641 gccatattgt gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg
    11701 tcattagtca gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca
    11761 atagtcgctt cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg
    11821 aaaacgtggc ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga
    11881 acggtcgtaa tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg
    11941 gtcacggtaa aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca
    12001 agttgtttac ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg
    12061 gtcagcgcca tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt
    12121 tcacggtctt catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca
    12181 ttttcaacac gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa
    12241 aaacctcact aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag
    12301 ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc
    12361 taaccgtcgg ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc
    12421 tcgcgggctt tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa
    12481 taagcggatt cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc
    12541 tggtttatga cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa
    12601 actacaatga ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg
    12661 cctaaaggaa tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa
    12721 gctcagctga cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc
    12781 ctgttacgga ctcgtgagaa actggcgctg cttgaacaaa gaatcagcga actggaaaat
    12841 cgcagtactg agatcaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca
    12901 tttgtcttta atgcactgta aattatgaat taatcctcta atttacagtg ttttctatta
    12961 cattatcgct taatcccatt cagagtatca tcacaaaaat gaatgattgc aattgaaaat
    13021 ataattgtta tgtattcttt aagttttact taatcgata
//
LOCUS       DAZPFP010000102        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPFP010000102 DAZPFP010000000
VERSION     DAZPFP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.102"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUR4_002701"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUR4_002701"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1456224.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHUR4_002702"
     CDS             complement(1806..3224)
                     /locus_tag="ACHUR4_002702"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1456225.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHUR4_002703"
     CDS             complement(3378..3722)
                     /locus_tag="ACHUR4_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1456226.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUR4_002704"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUR4_002704"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1456227.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHUR4_002705"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHUR4_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUR4_002706"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUR4_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1456228.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUR4_002707"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUR4_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1456229.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUR4_002708"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUR4_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1456230.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHUR4_002709"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHUR4_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1456231.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHUR4_002710"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHUR4_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1456232.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHUR4_002711"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHUR4_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1456233.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUR4_002712"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUR4_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR1456234.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUR4_002713"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUR4_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUR4_002714"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUR4_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR1456235.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUR4_002715"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUR4_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZPFP010000103        12647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPFP010000103 DAZPFP010000000
VERSION     DAZPFP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.103"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHUR4_002716"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHUR4_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1456236.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUR4_002717"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUR4_002717"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1456237.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUR4_002718"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUR4_002718"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1456238.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUR4_002719"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUR4_002719"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1456239.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUR4_002720"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUR4_002720"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1456240.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUR4_002721"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUR4_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHUR4_002722"
     CDS             7849..7902
                     /locus_tag="ACHUR4_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456241.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUR4_002723"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUR4_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1456242.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUR4_002724"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUR4_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1456243.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUR4_002725"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUR4_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1456244.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHUR4_002726"
     CDS             10966..12165
                     /locus_tag="ACHUR4_002726"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1456245.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3169 c         3359 g         3007 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    12361 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    12421 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    12481 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    12541 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    12601 tgccatgaga gtcgtgatat tattattcat catttttagg taatgct
//
LOCUS       DAZPFP010000104        12337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPFP010000104 DAZPFP010000000
VERSION     DAZPFP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.104"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            563..1333
                     /locus_tag="ACHUR4_002727"
     CDS             563..1333
                     /locus_tag="ACHUR4_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1456246.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="ACHUR4_002728"
     CDS             1324..1797
                     /locus_tag="ACHUR4_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1456247.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="ACHUR4_002729"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="ACHUR4_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1456248.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="ACHUR4_002730"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="ACHUR4_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1456249.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /gene="yjjA"
                     /locus_tag="ACHUR4_002731"
     CDS             3232..3726
                     /gene="yjjA"
                     /locus_tag="ACHUR4_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1456250.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="ACHUR4_002732"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="ACHUR4_002732"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1456251.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACHUR4_002733"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACHUR4_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="ACHUR4_002734"
     CDS             7556..8470
                     /locus_tag="ACHUR4_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1456252.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="ACHUR4_002735"
     CDS             8685..10046
                     /locus_tag="ACHUR4_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456253.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACHUR4_002736"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACHUR4_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR1456254.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACHUR4_002737"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACHUR4_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1456255.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         3042 a         3236 c         2962 g         3097 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctg
//
LOCUS       DAZPFP010000105        12184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPFP010000105 DAZPFP010000000
VERSION     DAZPFP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.105"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..602)
                     /locus_tag="ACHUR4_002738"
     CDS             complement(<1..602)
                     /locus_tag="ACHUR4_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1456256.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACHUR4_002739"
     CDS             1122..2021
                     /locus_tag="ACHUR4_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1456257.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUR4_002740"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUR4_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1456258.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="ACHUR4_002741"
     CDS             3494..4816
                     /locus_tag="ACHUR4_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456259.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="ACHUR4_002742"
     CDS             complement(4998..7058)
                     /locus_tag="ACHUR4_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1456260.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUR4_002743"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUR4_002743"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1456261.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUR4_002744"
     CDS             8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUR4_002744"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1456262.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="ACHUR4_002745"
     CDS             complement(9152..10648)
                     /locus_tag="ACHUR4_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1456263.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUR4_002746"
     CDS             complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUR4_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1456264.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2920 a         3310 c         3000 g         2954 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtct
//
LOCUS       DAZPFP010000106        12153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPFP010000106 DAZPFP010000000
VERSION     DAZPFP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.106"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHUR4_002747"
     CDS             126..995
                     /locus_tag="ACHUR4_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1456265.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHUR4_002748"
     CDS             complement(1198..1551)
                     /locus_tag="ACHUR4_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1456266.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUR4_002749"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUR4_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1456267.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHUR4_002750"
     CDS             complement(3379..3672)
                     /locus_tag="ACHUR4_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1456268.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUR4_002751"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUR4_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1456269.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHUR4_002752"
     CDS             complement(5220..5696)
                     /locus_tag="ACHUR4_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1456270.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUR4_002753"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUR4_002753"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1456271.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUR4_002754"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUR4_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1456272.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUR4_002755"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUR4_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1456273.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUR4_002756"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUR4_002756"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1456274.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHUR4_002757"
     CDS             11052..11627
                     /locus_tag="ACHUR4_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1456275.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHUR4_002758"
     tRNA            11734..11809
                     /locus_tag="ACHUR4_002758"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAZPFP010000107        12113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPFP010000107 DAZPFP010000000
VERSION     DAZPFP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.107"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..370
                     /locus_tag="ACHUR4_002759"
     CDS             <1..370
                     /locus_tag="ACHUR4_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456276.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHUR4_002760"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHUR4_002760"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1456277.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHUR4_002761"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHUR4_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1456278.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHUR4_002762"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHUR4_002762"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1456279.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHUR4_002763"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHUR4_002763"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1456280.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHUR4_002764"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHUR4_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1456281.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHUR4_002765"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHUR4_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1456282.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHUR4_002766"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHUR4_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1456283.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHUR4_002767"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHUR4_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1456284.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHUR4_002768"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHUR4_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1456285.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHUR4_002769"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHUR4_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1456286.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHUR4_002770"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHUR4_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1456287.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHUR4_002771"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHUR4_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1456288.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2933 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tatccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZPFP010000108        11934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPFP010000108 DAZPFP010000000
VERSION     DAZPFP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.108"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHUR4_002772"
     CDS             complement(<1..2251)
                     /locus_tag="ACHUR4_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR1456289.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHUR4_002773"
     CDS             complement(2275..2898)
                     /locus_tag="ACHUR4_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR1456290.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHUR4_002774"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHUR4_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUR4_002775"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUR4_002775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1456291.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUR4_002776"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUR4_002776"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1456292.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11934)
                     /gene="yfaL"
                     /locus_tag="ACHUR4_002777"
                     /pseudo
     CDS             complement(8356..11934)
                     /gene="yfaL"
                     /locus_tag="ACHUR4_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2759 a         3310 c         2948 g         2917 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc ggcgatagac tggccccctg aatc
//
LOCUS       DAZPFP010000109        11842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPFP010000109 DAZPFP010000000
VERSION     DAZPFP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.109"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHUR4_002778"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHUR4_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1456293.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="ACHUR4_002779"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="ACHUR4_002779"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1456294.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="ACHUR4_002780"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="ACHUR4_002780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1456295.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="ACHUR4_002781"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="ACHUR4_002781"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1456296.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="ACHUR4_002782"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="ACHUR4_002782"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1456297.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="ACHUR4_002783"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="ACHUR4_002783"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1456298.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="ACHUR4_002784"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="ACHUR4_002784"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1456299.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="ACHUR4_002785"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="ACHUR4_002785"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1456300.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHUR4_002786"
     CDS             7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHUR4_002786"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1456301.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHUR4_002787"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHUR4_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1456302.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHUR4_002788"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHUR4_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHUR4_002789"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHUR4_002789"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1456303.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         2958 a         3028 c         3019 g         2837 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt ta
//
LOCUS       DAZPFP010000110        11801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPFP010000110 DAZPFP010000000
VERSION     DAZPFP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.110"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHUR4_002790"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHUR4_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHUR4_002791"
     CDS             2265..3914
                     /locus_tag="ACHUR4_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1456304.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHUR4_002792"
     CDS             3919..4695
                     /locus_tag="ACHUR4_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1456305.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUR4_002793"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUR4_002793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1456306.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUR4_002794"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUR4_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1456307.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUR4_002795"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUR4_002795"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1456308.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2865 a         2842 c         3177 g         2917 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac a
//
LOCUS       DAZPFP010000111        11791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPFP010000111 DAZPFP010000000
VERSION     DAZPFP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.111"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(27..767)
                     /gene="cysQ"
                     /locus_tag="ACHUR4_002796"
     CDS             complement(27..767)
                     /gene="cysQ"
                     /locus_tag="ACHUR4_002796"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1456309.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            957..2900
                     /gene="cpdB"
                     /locus_tag="ACHUR4_002797"
     CDS             957..2900
                     /gene="cpdB"
                     /locus_tag="ACHUR4_002797"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1456310.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3026..3406)
                     /locus_tag="ACHUR4_002798"
     CDS             complement(3026..3406)
                     /locus_tag="ACHUR4_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1456311.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3495..4355
                     /gene="qorB"
                     /locus_tag="ACHUR4_002799"
     CDS             3495..4355
                     /gene="qorB"
                     /locus_tag="ACHUR4_002799"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1456312.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4464..5429
                     /locus_tag="ACHUR4_002800"
     CDS             4464..5429
                     /locus_tag="ACHUR4_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1456313.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5537..6199
                     /gene="ytfE"
                     /locus_tag="ACHUR4_002801"
     CDS             5537..6199
                     /gene="ytfE"
                     /locus_tag="ACHUR4_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1456314.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6244..7656)
                     /gene="cycA"
                     /locus_tag="ACHUR4_002802"
                     /gene_synonym="dagA"
     CDS             complement(6244..7656)
                     /gene="cycA"
                     /locus_tag="ACHUR4_002802"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1456315.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7964..8584)
                     /gene="fklB"
                     /locus_tag="ACHUR4_002803"
     CDS             complement(7964..8584)
                     /gene="fklB"
                     /locus_tag="ACHUR4_002803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1456316.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8803..9348
                     /locus_tag="ACHUR4_002804"
     CDS             8803..9348
                     /locus_tag="ACHUR4_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR1456317.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9332..9979)
                     /locus_tag="ACHUR4_002805"
     CDS             complement(9332..9979)
                     /locus_tag="ACHUR4_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1456318.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10191..10982
                     /locus_tag="ACHUR4_002806"
     CDS             10191..10982
                     /locus_tag="ACHUR4_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR1456319.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            10992..>11791
                     /locus_tag="ACHUR4_002807"
     CDS             10992..>11791
                     /locus_tag="ACHUR4_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1456320.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         2974 a         3070 c         2976 g         2771 t
ORIGIN      
        1 aaatacactg tttctgccat ctgaatttag taaatggaca ctctgaaccc cggattcagg
       61 aacgactcgc gcggagtgta atccagcggt ttaccctgcc agtcgtgaac gtgcgctccg
      121 gcagctgcag ccacagcatg tccggcggcg gtgtcccaaa tattcgttgg tccgaagcgc
      181 gggtacagct gcgcctgtcc ttccgccacc aggcagaatt tcagcgaaga gccgatggac
      241 gtggtctgat gttcgccaag ctgttgcaga tactctttca gctccgcatc tgcatgggaa
      301 cggctgataa ccaccagcgg cgggcgcgca tcgcggacct gaatcagctt gcgcacaccg
      361 cactcttctt tccaggcctt gccttctgcc gcgctgtaca ttacgttcat taccggcgca
      421 tacaccacac ctaaaatcgg tttgccatgg tcaatgagcg caatgttaac ggtgaattcg
      481 ccattacgtt taataaactc tttagtacca tccagcgggt ctaccagcca gtaacgctgc
      541 cagtgctgac ggacttccca accgggagga tcttcttcag aaaggaccgg aatatccggt
      601 gccagcgtac gtaaaccgtc catgataacg gtgtgagcgg caatatccgc tgccgttacc
      661 ggagaattgt ccgctttgct gacgacgtcc atcggtttcg tcccgtcgta gacctgcata
      721 atggcatcgc ctgcattccg tgcaagctgg catacttgat ctaacatttc tccacctcgt
      781 ctctgtgagc ggtgttaact tattgtttta cttataccct atcgttaatg aatgcgccaa
      841 ctgtgatagt gtcatcattt tcaaagcgta aaattgtggc attcttcact gttctataag
      901 taaggcgttt attctccctt ttctttcgta ttcccgatga taaaaggatg tccctgatga
      961 ttaagtttag cgcaacgctc ctggccacgc tgattgccgc cagtgtgaat gcagcgacgg
     1021 tcgatctgcg tatcatggaa accactgatc tgcatagcaa catgatggat ttcgattatt
     1081 acaaagacac cgccacggaa aaattcggac tggtacgtac ggcaagcctg attaacgatg
     1141 cccgcaatga agtgaaaaac agcgtactgg tcgataacgg cgatttgatt caggggagtc
     1201 cgctggccga ttacatgtcg gcgaaaggat taaaagcagg tgatgttcat ccggtttata
     1261 aggcgctgaa tacgctggat tatacggtcg gtacactcgg caatcatgaa tttaactacg
     1321 gtctggatta cctgaaaaat gcgctggcgg gagcgaaatt cccttatgta aatgccaacg
     1381 tcattgacgc cagaaccaaa cagccaatgt ttacaccgta tttaattaaa gataccgaag
     1441 tggtcgataa agacggaaaa aaacagacgc tgaagattgg ctatattggc gtcgtgccgc
     1501 cgcaaatcat gggctgggat aaagctaatt tatccggaaa agtgacggtg aatgatatta
     1561 ccgaaaccgt gcgcaaatac gtgcctgaaa tgcgcgagaa aggtgccgat gtcattgtcg
     1621 ttctggcgca ttccgggctg tctgccgatc cgtataaagt aatggcggaa aactcagttt
     1681 attacctcag tgaaattccg ggcgttaacg ccattatgtt tggccatgct cacgccgttt
     1741 tcccgggtaa agattttgct gatatcgaag gggctgatat caccaaaggc acgctgaatg
     1801 gtgttccggc ggtaatgccg ggcatgtggg gcgatcatct tggggtggtc gacttacaac
     1861 tcagtaatga cagcggtaaa tggcaggtga cgcaggcgaa agcggaagca cggccgattt
     1921 acgacatcgc caataaaaaa tccctcgcgg cggaagacag caagctggta gaaacactca
     1981 aagccgatca cgatgccaca cgccagttcg tcagcaagcc aatcggtaaa tctgccgaca
     2041 atatgtatag ctatctggcg ctggtgcagg acgatccgac cgtgcaagtc gtgaacaacg
     2101 cgcaaaaagc gtatgtcgaa cattacattc agggcgatcc ggatctggca aaactgccgg
     2161 tgctttcagc tgccgcaccg tttaaagttg gtggtcgcaa aaatgacccg gcaagctatg
     2221 tggaggtgga aaaaggccag ttgaccttcc gtaatgccgc cgatctttat ctctacccca
     2281 atacgctgat tgtagtgaaa gccagcggta aagaggtgaa agagtggctg gagtgctccg
     2341 ccggacagtt taaccagatt gctcctaaca gcacgaaacc acagtcactc atcaactggg
     2401 atggtttccg cacttataac tttgatgtta ttgatggtgt gaattatcag attgatgtta
     2461 cccaacccgc ccgctatgac ggcgagtgcc agatgattaa tgccaatgca gagcggatta
     2521 agaacctgac ctttaatggc aagccgattg atccgaacgc tatgttcctg gttgccacca
     2581 ataactatcg cgcttacggc ggcaaatttg ccggtacggg cgacagccat atcgcttttg
     2641 cttcaccgga tgagaaccgc tcggtgctgg cagcgtggat tgctgatgag tcgaaacgtg
     2701 cgggggaaat tcatccggcg gcagataaca actggcgttt agcaccgata gctgccgata
     2761 agaaactgga tatccgtttc gaaacttccc cgtcagataa agccgcagcg tttattaaag
     2821 agaaagggca gtatccgatg aataaagtcg cgaccgatga tatcgggttt gcgatttatc
     2881 aggtggattt gagtaagtaa aacacttctt tttcggccta taaatcaacc gcatccggca
     2941 tttattggcg gatgcgatgc tgccgtatct tatccaccct acaagccatg aaccgtagac
     3001 cggataagcg cagcgcatcc ggcagttatg ccgcacgttc atcccgcacc gccaacacct
     3061 cgggcaaatt caactcaatc cagtccgcca gtgcagcaac cttttcgctc acctgctcgc
     3121 ccagcggcgt gaggctatat tccacatgcg gcggcaccac cggatacgcg atacggttaa
     3181 gaaaaccatc ctgttctaac gcctgtaacg actgcgcaag catcttttca ctcaccccac
     3241 cgattttgcg ccgcaggtcg ctaaagcgat gagtaccttc gcgtagcgcc accagaatca
     3301 acacccccca acggctggtg acgtgtttca acacctcgcg cgacgggcac tgttccgcaa
     3361 agagattacc ctctttcagt tgttgtgaca ggctaacctg actcatttca tacttacctt
     3421 tttgcacgta cttactaaaa gtaagtttag gtgttagcgt atttaaacac aagacaaaac
     3481 gatggagact tcccatgatc gctattactg gtgccactgg ccaacttggt cactatatta
     3541 ttgaatcctt gatgaaaacg gttcctgcca gccaaatagt ggctatcgtt cgtaatccgg
     3601 caaaagccca ggccctggca gcacaaggca ttaccgtgcg tcaggctgac tacagcgatg
     3661 aagccgcact gacatctgca cttcagggag tggaaaaact actgctgatc tcttccagcg
     3721 aagtgggtca acgtgccccg cagcatcgta atgttattaa tgccgcaaag gcggctggcg
     3781 tgaaatttat cgcttatacc agcctgctac atgcagatac ctccccgctc ggcctcgccg
     3841 atgagcacat cgagacggag aaaatgctgg ctgattctgg catcgtttac accctgctgc
     3901 gcaacggctg gtacagcgaa aactacctcg ccagcgcccc ggcagcactg gaacacggcg
     3961 tatttatcgg tgcggctggc gatggcaaaa tcgcctcagc aacgcgggca gattatgcga
     4021 cagctgcggc acgcgtgatt agcgaagccg gtcacgaagg caaggtttac gaactggcgg
     4081 gcgatagtgc ctggacgttg acacagttag cggcagagct gaccaaacag agcggcaaac
     4141 aggttaccta tcaaaatctg agcgaagccg atttcgccgc ggcactgaaa agcgtcggac
     4201 tgcccgacgg actggcggat atgctggcgg attctgacgt tggcgcatcg aaaggtggtc
     4261 tgtttgataa cagcaaaacg cttagcaaat tgattggctg cccaacgaca acgttagccg
     4321 aaagcgtaag ccatcttttt aatgttaata actagttaat taaagtggca tcctcccgca
     4381 tcctctctga taatgacggg atgccgggag caatcatgtc tgcttcctga acgtttcttc
     4441 tgacagacca atggatgcca gtaatgatta gcggcgtgct gtacgccctg ttagcagggt
     4501 tgatgtgggg gcttattttt gtcgggccgt tgatcgtgcc ggaatacccg gcgatgttgc
     4561 agtcgatggg gcgttatctg gcgttagggt taattgcgct acccattgcc tggctgggac
     4621 gcgtgcgtct gcgtcagttg gcgcgtcggg actggcttac cgccttgatg ctcactatga
     4681 tgggcaacct catttattac ttctgccttg ccagtgccat tcaacgtact ggcgcgcctg
     4741 tttccacgat gattatcggc accctgccgg tggtgattcc tgtctttgcc aatctgcttt
     4801 atagccagcg cgacggcaaa ctcgcgtggg gaaaactcgc cccggcactg atttgtattg
     4861 gcatcggcct ggcgagtgtg aatattgctg agttaaacca cggactcccc gattttgact
     4921 gggcacgtta tacctctggc atcgtgctag cgttagtttc cgtggtctgc tgggcatggt
     4981 atgccctgcg caacgcccgc tggctgcggg aaaatcccga caaacatccg atgatgtggg
     5041 cgacggcgca ggcgctggtc acgctgccgg tttctctcat cggctatctc gtcgcctgtt
     5101 actggctgaa tatacaaacg ccggacttct ccttaccttt tggcccccgt ccgctggtgt
     5161 ttattagtct gatggttgcg atagccgtgc tttgctcatg ggttggcgca ctctgctgga
     5221 acgtcgccag ccagcgatta ccgacagtga ttctcgggcc gctgattgtt ttcgaaacgc
     5281 tggcaggttt gctgtacacc tttttactcc gccagcaaat gccgccgcta atgacgctga
     5341 gcggtatcgc gctgttagtg attggcgtgg tcatcgcggt cagagcaaaa ccagaaaaac
     5401 ctttaactga atctgtctca gaaagttgac atgctggcag tgagttaaat aagcctctgc
     5461 tacgtaaggg ttatagcttt tgccttaaag gtgtatttaa aatacatctt atcttattaa
     5521 gaatgaggta tcagctatgg cttatcgcga ccaaccttta ggtgaactgg cgctctctat
     5581 ccctcgcgct tcggctctgt ttcgtaaata tgatatggat tactgctgtg gcggtaagca
     5641 gacgctggcg cgcgcggcgg cacgtaaaga actggatgtt gaggtcattg aagctgaact
     5701 ggcaaagctt gctgaacaac cgattgagaa agactggcgt agcgccccgc tggcagaaat
     5761 cattgaccat atcatcgtgc gctaccacga tcgtcaccgc gagcagctgc cggagctgat
     5821 cctgcaagcg actaaagtcg agcgcgttca cgccgacaaa ccgagcgtgc caaaagggct
     5881 gacaaaatac ctgaccatgc tgcatgaaga gctttccagc cacatgatga aagaagagca
     5941 gatcctcttc ccgatgatca aacaaggcat gggcagccag gcaatggggc caatcagcgt
     6001 aatggaaagc gagcacgatg aagcgggcga actgctggaa gtgatcaaac acactaccaa
     6061 taacgtcaca ccgccgccag aagcctgcac cacctggaaa gcgatgtata acggcattaa
     6121 tgaactgatt gatgacctga tggatcacat cagtctggaa aacaatgtac tgttcccacg
     6181 cgccctagcg ggtgagtgat ctaaaagctg gatggcattg cgccatccag catgataatg
     6241 cggttatttc cgcagttcag cagcccgctt cttaccaata aacagccagc ccaaccccag
     6301 cgcgataaac cacagcgggg taaccagcag cgcctggcga gtgtcatctt ccagtgtcag
     6361 caacacgacc acgaacacaa agaacgccat acatacccag cacatcagct tgccgagcgg
     6421 catcttgtag atcgactttt catgcagatg aggacgctgt ttgcggtaca ccaggtaaga
     6481 gcaaaggata atcgtccaga cgaacataaa cagaatcgcg gaaacggttg taatcatcgt
     6541 gaacgcgcca atcacgctag gattcacata caacatcacc acgccgccga gcagacagat
     6601 acacgagaag gtcaaccctt tcgcgggtac tgcgcgctta gaaagtttag cgaacgcttt
     6661 cggcgcaaca ccttcctgcg ccagaccaaa cagcatacgg ctggtagaga agacaccgct
     6721 gttagcggaa gacgccgcag aggtcagcac cacaaagttg atcacgctgg cagccgcagg
     6781 caaacctacc aacacaaaca gctcaacgaa cgggcttttc tccgggacta ccgaactcca
     6841 cggtgtcacg gacataatca caatcagcgc gaagacgtag aacataatga tacggatcgg
     6901 aatggagtta atcgcgcgtg gcagtgattt ctccggatct ttggtttccg cagctgttgt
     6961 acctaccagc tcaatcccca cgaaagcgaa aaccgctatc tggaatccgg caaagaagcc
     7021 acttaaacct ttcgggaacc agccgccgtc attccacaaa tgcgcgaatg acgcttccac
     7081 accggtcggt gactgaaagt gcatcgccac catgaccagg ccgacgacaa tcagcgacac
     7141 gatggcgacg attttgatca tcgcaaacca gaactccatc tcaccgaaca ttttcacggt
     7201 ggcaaggttg agcgtcagca gcagcactat caccgccagc gaggcgaccc agtcggagag
     7261 atcggggaac cagaactgag cataagccgt gatcgccacc acgtctgcca taccggttac
     7321 aacccagcag aaccagtaag tccagccggt gaaatatccc gcccacggcc ccagtaaatc
     7381 ggaagcgaag tcactaaaag atttgtattc cagattcgaa agcagcaatt cccccattgc
     7441 ccgcatcacg aaaaagagca taaaaccaat gatcatataa acgaaaatga tcgacggccc
     7501 ggcaaggcta atcgttttgc cggaccccat aaacaaccca gtaccaatgg cacctccaat
     7561 ggcaataagc tgaacatgtc ggtttgtgag attgcgccgt agcgactgtt cagccggagc
     7621 ctgatcatcg gcaacgactt ttacctgatc taccatgttt tttcttcctg tacctgtctg
     7681 tgttgttcag gctctacggc ctttagtcag tctatgatac gacgaatctg tatcgggact
     7741 catcgatatt aggtaacaat acgcgggatg aatactaaga tttagatcta atgttaattt
     7801 tatgtttaaa gtgagtgtca tatcacggtg ataacgcgaa acagctcaca aaaatacacg
     7861 cttacggggt ctttgcaaca caaaatagcg ccgtttggca aactgaaggg tttattgctg
     7921 aatgcctgct cccctctggt tcgaggggaa cagaatgcgc tgcttagagg atttccagca
     7981 gttcgacttc aaacaccagg gtgctgaacg gagggatgga tgcacctgcg ccgcgctcgc
     8041 catatgccag ttcctgcggg atagtcagtt cccatttaga acctaccggc atcagagtca
     8101 gtgcttcaat ccagccaggg atcacgccat taaccgggaa ttcagcgggt tcaccacgag
     8161 caacggagct gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc
     8221 ggtcggtacg tgccggaatt gcgccttcac cctggttgat cacgcggaat tgcaggccag
     8281 attcggtgct attcacacct tcttttttgg cgttttcttc caggtatttc acgccttcag
     8341 cagccatcgc ctggaaacgc tgacgacgaa cggcatcggc gcgttcgtgg atttcacgca
     8401 gcgcgcgatg caccacatca accggcacag ccggatgttt gccttccagc gcatcggcaa
     8461 tacctgcaac cagtgcttct ggcagcagcc cttccaggcc agattcactc agttgttgcc
     8521 ctacctgcaa accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg
     8581 tcatcgtatt tcctttctta gggtataaaa gtagcgggca gcatatcagc cacacttctt
     8641 tgggtaaaac tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa caatagcaat
     8701 atcccgtatg aatataataa aggcgggttt accacggaca tcatagcgtt agcttattca
     8761 taggctatga ttgaggaaca agacgtggag caggaggaaa ccatgcccgg gcgctttgaa
     8821 ctaaaaccaa ccctggagaa agtctggcac gcgccggaca attttcgctt catggacccg
     8881 ctgccgccga tgcatcgtcg ggggatcatt attgccgcca ttgtattggt ggtcggattt
     8941 ctgcttccat ctgatgatac gcccaacgcg tcggtggtga cgcgtgaagc gcagttagat
     9001 attcagtcgc aatcacaacc gccgacagaa gaacagccgc aaactaccca aacgcaacca
     9061 ttccagccag atagcggaat tgataatcaa tggcgctctt accgtgtcga acccggtaaa
     9121 acgatggcgc agctgttccg cgaccacgga ctgcccgcta ccgatgtcta tgcaatggcg
     9181 caggtagaag gcgcgggtaa gccgctgagt aatttgcaaa atgggcagat ggtgaaaatc
     9241 cgccagaacg ccagcggcgt ggtgacgggc ttaaccattg atacggggaa taatcaacag
     9301 gtcctgttta cccgccagcc ggatggcagt tttattcgtg cgcggtagat gaagggtcaa
     9361 ccaaaagcca gctaaggatt gtctctttga tgaccttacg tctggcttcc agtgcctggg
     9421 gggaaaccat actgatatga taaactaggc caagcgtatg ctggttgatc aaataatatc
     9481 ccccaagcgc agcaatatta atattcacct gcaatgggtc gatacctggc ttaaaaatat
     9541 tgtatttctt cccttcatcc aataaggatt ccattaactg caagtgagca tggttaattt
     9601 ctaagagacg ctgtgatttt gcataatgca cacctttact ttgattttca ctatgcacaa
     9661 tttttaaaaa ccaaggatta gcaatataat aatcccatgt aaaatcaata attttctcaa
     9721 tggctttttg tggcggaaga tctgagaaat caagggcctt ttctttttgt cgaatatcgt
     9781 tccaggtata ttccagcact tcgataaata aattttcttt attaccaaag tggtgataaa
     9841 ccgtctgttt actacaccct gctgccttta caatattatc tactctggcc ccttcataac
     9901 catattcggc aaattcatta accgcacata gcagcaattt ttccttcagg ctctgtacgt
     9961 tgctggtatc aatatacata acgcttcccc acgacctctt cgtggtgact atcataacct
    10021 gatttgattt cagcttcaga aaaaagggca aaaacttgta ctttcatcag gttatcaaat
    10081 gtcctgtgat cgccatcgca tatcaaccaa ctagatggtt tatcttttta tcctccagtc
    10141 gcaagctaat catcaccaag gaaacaaaat tcaaaaaaga gagctattgt atgaaatcca
    10201 ctcgtcctgt tgccgtgatt accggagcca ctcgcggtat tggaaagggc tgtgcgcagg
    10261 agcttgctcg tggcgggttt aacctgctga ttaacgatcg tcccgatgcc gacagcgtag
    10321 aaaaactgca tatcacacaa caagaatgtt tggcggaagg cgtggaagtg atttgcttcc
    10381 ctgctgatgt cggtgatcta tctctgcatg aggagatgct cgacgcggcg caaaaccagt
    10441 gggggcggct ggactgtctg ctcaataacg cggggatttc agtgaaaaaa cgcggtgacc
    10501 tcctcgacct cgaaccagac agcttcgatc aaaacattgc cattaacacc cgcgcgccgt
    10561 tcttccttgc tcaggcattc agtaagcgct tactggcaca gccgaaaccg gaagcggagc
    10621 tgccgcaccg ctcgattatt ttcgtcagtt ccatcaacgc catcatgctg gcgatgaatc
    10681 gcggcgaata caccattgcc aaaaccgccg tttctgccgc tgctcgtctg tttgctgctc
    10741 gcctttgtaa tgaacaaatt ggcgtttatg aagtgcgccc tggtctcatc aaaactgata
    10801 tgactattcc ggcaacggct tattacgacg aactgattgc caaaggtctg gtgccgtggg
    10861 gacgttgggg ttatccggct gatattgctt ccaccgtccg cgcgatggcg gaaggtaaac
    10921 tgatttacac ctgtggtcag gcggtcgcaa tcgacggcgg cttaagtatg ccgcgcttct
    10981 gaggctgaat catgagagat ctgttacaac gcccggattt attcagtatc aataccgcga
    11041 cactgggcta taaaacgccg ctacccgcca ttattgatgc ctgcgccgca cgcggtattg
    11101 gcgcaattgc cccctggcgc agggagttgc agggtgaaga tctccagcaa atcacccgcc
    11161 agcttgccgc cagtaacatg agcgtttccg gtttgtgccg tagcacttac tacaccgcgc
    11221 cgacactggc ggaacgcaaa ctggcgattg atgataaccg ccgggcgctg gacgacgcag
    11281 cggtgcttaa cgccgcctgt tatatgcagg tggttggcgg tctgccaacg ggcactaaag
    11341 atctatatga agcgcgagaa caagtgaaac aagggattcg ccagttgctg ccacattcga
    11401 aagatgtcgg cgtgccaatt gcgctggagc cactgcaccc gatgacagct gccgatcgct
    11461 cttgcctgtg cacgttacgt caggcgctgg actggtgtga tgaactcgat cccgatggtg
    11521 agtttggcct cggcgtggcg gtggatgttt atcacgtctg gtgggatccg gatctcgcca
    11581 gtcagatcct gcgtgccgga aaacgcatcc tcgcgtttca tgtttccgac tggttaatgc
    11641 cgaccactga tctggtcaat gaccgtggta tgccgggaga tggcgtcatc aatattccgt
    11701 caattcgtcg gctggttgaa aacgccggat ttaatggtgc tattgaactg gaaatattct
    11761 cgccttactg gtggcagaaa gatattaaca g
//
LOCUS       DAZPFP010000112        11716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPFP010000112 DAZPFP010000000
VERSION     DAZPFP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.112"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHUR4_002808"
     CDS             <1..851
                     /locus_tag="ACHUR4_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1456321.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHUR4_002809"
     CDS             873..2282
                     /locus_tag="ACHUR4_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456322.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHUR4_002810"
     CDS             2260..3039
                     /locus_tag="ACHUR4_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1456323.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHUR4_002811"
     CDS             3036..3896
                     /locus_tag="ACHUR4_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1456324.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHUR4_002812"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHUR4_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHUR4_002813"
     CDS             complement(4636..4896)
                     /locus_tag="ACHUR4_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1456325.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHUR4_002814"
     CDS             complement(4887..6158)
                     /locus_tag="ACHUR4_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1456326.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUR4_002815"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUR4_002815"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1456327.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUR4_002816"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUR4_002816"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1456328.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUR4_002817"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUR4_002817"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1456329.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUR4_002818"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUR4_002818"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1456330.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHUR4_002819"
     CDS             11500..11652
                     /locus_tag="ACHUR4_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1456331.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2811 a         2989 c         3235 g         2681 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc cttttt
//
LOCUS       DAZPFP010000113        11682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPFP010000113 DAZPFP010000000
VERSION     DAZPFP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.113"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            47..610
                     /locus_tag="ACHUR4_002820"
                     /pseudo
     CDS             47..610
                     /locus_tag="ACHUR4_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUR4_002821"
     CDS             complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUR4_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1456332.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUR4_002822"
     CDS             complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUR4_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1456333.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUR4_002823"
     CDS             complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUR4_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1456334.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUR4_002824"
     CDS             complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUR4_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1456335.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUR4_002825"
     CDS             2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUR4_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1456336.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUR4_002826"
     CDS             complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUR4_002826"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1456337.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUR4_002827"
     CDS             complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUR4_002827"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1456338.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUR4_002828"
     CDS             complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUR4_002828"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1456339.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUR4_002829"
     CDS             complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUR4_002829"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1456340.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUR4_002830"
     CDS             complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUR4_002830"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1456341.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUR4_002831"
     CDS             complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUR4_002831"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR1456342.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUR4_002832"
     CDS             7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUR4_002832"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1456343.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUR4_002833"
     CDS             8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUR4_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1456344.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUR4_002834"
     CDS             complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUR4_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1456345.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUR4_002835"
     CDS             10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUR4_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1456346.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUR4_002836"
     CDS             10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUR4_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1456347.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11336..>11682)
                     /locus_tag="ACHUR4_002837"
     CDS             complement(11336..>11682)
                     /locus_tag="ACHUR4_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1456348.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         2959 a         3087 c         2870 g         2766 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtaa caatgaatgg
       61 tgataattct tttggatgga accatgaagc tacattagat aatattcacg cccagaaata
      121 caataaagct tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt
      181 cgaatccttc gaacccaaca gtaaatacac aatacacgag gttgttttgg gacctggtta
      241 tagtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat
      301 gcctaattgc tgctcatctg aactaccaac aatcgatctt tatatcgacc aacttcacac
      361 tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc
      421 tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag ccgcaatcta
      481 ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg atcgtatgca
      541 tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt
      601 gcgaaattaa tgcctgacga aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt
      661 acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact
      721 tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc
      781 agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg
      841 cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca
      901 gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt
      961 ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga
     1021 acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga
     1081 tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc
     1141 tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca
     1201 gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg
     1261 taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag
     1321 cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac
     1381 gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca
     1441 tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg
     1501 gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca
     1561 ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga
     1621 tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc
     1681 ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc
     1741 ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg
     1801 tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg
     1861 ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc
     1921 ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc
     1981 agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca
     2041 gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa
     2101 acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt
     2161 cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat
     2221 agtactggca taccccgcta aactcaaggg cggagcagaa ataaatctcc cctttcgcaa
     2281 ataacctcta aaccgctgat ttgaagttca tcacattccg tcacagtgtt ttttgaacaa
     2341 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     2401 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     2461 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     2521 aaccaccagg ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat
     2581 aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagaaacaga tgcgcaaggc
     2641 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     2701 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     2761 tgcccccttc gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc
     2821 cgaggaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggaccg
     2881 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tatgattgag ttgtggatta
     2941 atactgcgcg caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc
     3001 gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc
     3061 gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca
     3121 tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatatc accaaagaag
     3181 tagtcagcgt gcgtggtgcc taatgccggg atcgccagcc ccgcctgcgc ccatgcggtg
     3241 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg ggtaacgacg gtagagttcg
     3301 agatgcgtcg cagtgtcgga agatgggcga tactcccctt ccaccacctt gccgctcata
     3361 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc
     3421 accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaagtaaa ggtcaccagc
     3481 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     3541 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacac gctttcacca
     3601 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     3661 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc caccacacct tcgccaaatg
     3721 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     3781 ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg
     3841 ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg
     3901 agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca
     3961 tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     4021 gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc tgggcgaact
     4081 ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcgctgccca
     4141 acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg gtttcaacaa
     4201 tagcattcac cagcgccaga cgctgttcgc ggctccagtc aaggcgcgac aggcgctcgt
     4261 tagtttcatc taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agtcgcaggc
     4321 gttccagcca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     4381 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     4441 cggcttcaac cggagaagcg gcatcacgga tgctgcgacc ggcgataaac acatgaattg
     4501 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga
     4561 agcccatatc ggaaagacgt ttgatcgcag tgatatcggc ttcaccccac gccacgccag
     4621 cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatacctgcg tcgcgccact
     4681 gttgcgcctg ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact
     4741 cttttgctac gtccagcgca cctttggcgg tgttgatatc cgcacaacaa atcacggtca
     4801 cccagtcagc gttggcttcg aagcacatac gagaaagaat tttgcccgca tcggcaattt
     4861 tggcgtctgc cagcacgatt ttgtgcgggt agagcgcttt cagatcgcga accgcacgca
     4921 cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca
     4981 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg
     5041 gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     5101 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg
     5161 ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc cgccatggtg
     5221 atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt tttcagcgtc
     5281 accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     5341 cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag
     5401 taacgcggct cgacaacatc cgccgccacc agcaggtcaa caccgatttt tactgcgtcc
     5461 tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcatgt
     5521 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     5581 agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac gtatttgttg
     5641 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     5701 aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat gtttgattgg
     5761 gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac
     5821 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccaat
     5881 tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt
     5941 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     6001 ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc ggtaagtttc
     6061 accacccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     6121 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     6181 gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca
     6241 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg agcgaagcta
     6301 taaatagccg cacagtcaat cgccagaacc gcaccaggga tcaggcgctg ggagataccg
     6361 ttaaacgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     6421 gccaccgcga aggaaaaacc ggtttgcagg atatagaccg tccagtgcac tttgcctgcc
     6481 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     6541 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     6601 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aattaatgat
     6661 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     6721 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     6781 atggttgtcc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     6841 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     6901 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     6961 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     7021 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     7081 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     7141 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacagg
     7201 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     7261 atctcctgaa gaagaggtaa ccgggtattc cgcacacccg gaatgttgtg cactcactct
     7321 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     7381 aattccacat ggatagagtg ggattcacac aacgaaacaa ttatttattt aaattcattt
     7441 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
     7501 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
     7561 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     7621 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     7681 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     7741 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     7801 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     7861 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
     7921 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     7981 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     8041 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     8101 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     8161 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
     8221 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
     8281 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     8341 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     8401 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     8461 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     8521 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     8581 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     8641 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     8701 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     8761 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     8821 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     8881 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     8941 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     9001 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     9061 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     9121 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     9181 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     9241 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     9301 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     9361 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     9421 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     9481 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     9541 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     9601 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     9661 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     9721 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     9781 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     9841 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     9901 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     9961 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
    10021 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
    10081 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
    10141 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
    10201 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
    10261 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
    10321 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
    10381 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
    10441 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
    10501 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
    10561 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
    10621 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
    10681 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
    10741 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
    10801 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
    10861 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
    10921 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
    10981 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
    11041 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
    11101 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
    11161 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
    11221 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
    11281 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
    11341 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
    11401 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
    11461 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
    11521 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
    11581 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
    11641 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZPFP010000114        11514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPFP010000114 DAZPFP010000000
VERSION     DAZPFP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.114"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHUR4_002838"
     CDS             38..778
                     /locus_tag="ACHUR4_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1456349.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHUR4_002839"
     CDS             837..1223
                     /locus_tag="ACHUR4_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1456350.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHUR4_002840"
     CDS             1232..1561
                     /locus_tag="ACHUR4_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1456351.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHUR4_002841"
     CDS             1533..4574
                     /locus_tag="ACHUR4_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1456352.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHUR4_002842"
     CDS             4574..4903
                     /locus_tag="ACHUR4_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1456353.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHUR4_002843"
     CDS             4903..5601
                     /locus_tag="ACHUR4_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1456354.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHUR4_002844"
     CDS             5606..6349
                     /locus_tag="ACHUR4_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1456355.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHUR4_002845"
     CDS             6313..6888
                     /locus_tag="ACHUR4_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1456356.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHUR4_002846"
     CDS             6949..10428
                     /locus_tag="ACHUR4_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1456357.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHUR4_002847"
     CDS             10496..11095
                     /locus_tag="ACHUR4_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1456358.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11117..>11514
                     /locus_tag="ACHUR4_002848"
     CDS             11117..>11514
                     /locus_tag="ACHUR4_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1456359.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2694 a         2717 c         3703 g         2400 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggc
//
LOCUS       DAZPFP010000115        11488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPFP010000115 DAZPFP010000000
VERSION     DAZPFP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.115"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(101..2857)
                     /gene="barA"
                     /locus_tag="ACHUR4_002849"
     CDS             complement(101..2857)
                     /gene="barA"
                     /locus_tag="ACHUR4_002849"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1456360.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            2914..4215
                     /gene="rlmD"
                     /locus_tag="ACHUR4_002850"
     CDS             2914..4215
                     /gene="rlmD"
                     /locus_tag="ACHUR4_002850"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1456361.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4263..6497
                     /gene="relA"
                     /locus_tag="ACHUR4_002851"
     CDS             4263..6497
                     /gene="relA"
                     /locus_tag="ACHUR4_002851"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1456362.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            6593..7384
                     /gene="mazG"
                     /locus_tag="ACHUR4_002852"
     CDS             6593..7384
                     /gene="mazG"
                     /locus_tag="ACHUR4_002852"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1456363.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            7612..9249
                     /gene="pyrG"
                     /locus_tag="ACHUR4_002853"
     CDS             7612..9249
                     /gene="pyrG"
                     /locus_tag="ACHUR4_002853"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1456364.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            9337..10635
                     /gene="eno"
                     /locus_tag="ACHUR4_002854"
     CDS             9337..10635
                     /gene="eno"
                     /locus_tag="ACHUR4_002854"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1456365.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(10695..11488)
                     /locus_tag="ACHUR4_002855"
                     /pseudo
     CDS             complement(10695..11488)
                     /locus_tag="ACHUR4_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT         2928 a         2766 c         3005 g         2789 t
ORIGIN      
        1 gataaggcgt taacgccgca tccggcataa acacaggcac tttgtcacca atctgaaacc
       61 agcgtcataa aaagccgatt gctactcgac aagacgtccg ttacccgaga attttgctgg
      121 cttcgcgcgc gacattatcc atctcgtcca acagttccag cagctccggc tccaaatctt
      181 cttctttagt accgctacgt agctgttgtt cgatcagctg gcagagattc ttcatacgtg
      241 gtacgccgct atagccgcaa ctgccatgca gtttatgaat caaatcaacc aggccttccg
      301 ggttttctcc agccagctgt tcctcaactt tgttgcgaac ttcaggcagg aaatcgagta
      361 acatttgcag catatcgcgc gctaaatcgg tttttcctgc tgcctggcgt agtgccagtt
      421 gccagtcgag ggtcgcattc gggttcacca caatttcgtt gacttcgggc gtcacgacgc
      481 gagacgaaat accgctgcca ggcttgtagc gcaacaacaa attatgcaat cgctcttctt
      541 caatcggttt cgccagataa tcgctcatcc ctgcgccaag cagcttctct ttttgcccgg
      601 ccattgcatg cgccgttacc gcgataaccg gcgtttgtcg ctgatgcggg agttggtgga
      661 tgagttcgca ggcccgaatg ccatccatgt ccggcatttg aatatccatt aagatcaaat
      721 cgaacggcat ctgtttcgcc cgttcaactg cctgatgccc gctatcgcaa agttccacat
      781 gttgcaccat atcttccagc aatgcgccga taagtttcag gttagcgggg ttgtcatcaa
      841 ccgccatgac tgtcattgcc agcttacttt catcggttac aggcaaaagc gtgttttgtt
      901 tgtgatgaca aaattccgtc agggcaggca acaggcgcgt aggtgttaat ggtttcagca
      961 gacacgcgcc gataccatct tgcttgagtt tttcagcatt aacttgtgca tggcaaggaa
     1021 gtgccagcat caggaaatcg gtcatcgata ccgctttcgc taatcgctca tgttgcattg
     1081 ttagcggctc gcggaaggtc accgcgatgc ctaacaacat catgtcgtaa tgcgcgggag
     1141 gcagcgcgga gaacgttggg ctataaacca cttccagcgg cgtttcactt aaaatatcca
     1201 gcgtgcattg cgctgctgcg gagtttggtt cgacataggc caggcgctta cctgcgaggc
     1261 actggatgga tggcccttcg ataataatgt tcgggttcag atcgagatta atgtggaacc
     1321 agaaagttga accgcgattc ggctggctat ggaacgaaat atcgccgccc atttcattaa
     1381 ccagtttttg tgtaatcacc agccccagcc cggtgccacc atgacggcgg gaaatactgg
     1441 catcagcctg tcggaaggcc tggaataagc gcgattgatc gcgttcagga ataccaatgc
     1501 cggtatcccg aatctgcact tcaatctgca ctttggtatt actcagcgca cgtttttcta
     1561 ccagaatatc aatgttgcca ttctcggtga atttaattgc attccccacc aggttagtga
     1621 tgatttgctg taatcgcagt gggtcgccga tcacgttatc aggcacgtcg cttttaatat
     1681 tgagcgtcag ttccaaccct ttatcgtgag aagaatgtgc cagcagagta acgacttcat
     1741 ccagcgtgct gcgtagtggg aatggaatac tttccagaat cagcttacct gcttccagtt
     1801 tcgagaagtc gagaacatca ttaataattg ccagcaaatt atttgccgaa cgttcaatcg
     1861 tattcaggtg atcgcgctgc gttggtgtta attctgtttt cagcgtcagg cgggtaaagc
     1921 caataacacc attcagtggt gtacgcagct cgtgtgacat atttgccaga aactcggatt
     1981 taatacgcgc cgcttcctgg gcgcgctttt tcgccagatc caactcaacg ttctgaattt
     2041 ccatctgctc cagcgtttca cgcagatcgg acgtcgcctg gtcgatattg tgctgcatct
     2101 cttcgtgata agcagccagc gacattgcca tcgagttgat accgtttttc agcatatcca
     2161 gctcgccgag cataaatcct tccacccggc tgtcgagttg cccgcgacgg atgcggtcga
     2221 cggtattcac catgttgcga atcggaccgg ttacatcgcg cattaagcgc cagccaaaaa
     2281 taagcgcaat accgatacaa aacagcatca tcacgcagga aataaagatc tctttatatt
     2341 gctgcaagcg aaccgattta agatccagct caagcgcaat atatcccaac atattttgac
     2401 tatttttggc atcgctactg ggcgattcgt cgggggagta actctcagaa ataatcggcg
     2461 tgcggaggat cataatatcg ccgtcacgag tgacagtgag ctggcgagga aacggcacgt
     2521 tgctgccgag ctgcattgat gagggatcaa gatgaaaatt ggaggtgaca aagagtcggt
     2581 tattttcatc ataaaccgaa atcgcgcgaa caatatcgga atggcgacga tgcagtacgc
     2641 ttattaactg accgatagat tcgcgatttt gcaggctcat gccatattca gtagaaactg
     2701 caagcggctc aataatgcta gcaccggcat cttccagttg acgctgcaag tcgttatagc
     2761 gatgcacgac gaaaaagata ctcagcaata aaccaataag gacggtcggt gccaggatca
     2821 gaatcatcat gcgtgcgcgc aggctgtagt tggtcatgga gttccgttat gggacaatta
     2881 aggtcacact gttaaattga gaaagtctcg ccaatggcgc aattctactc tgcaaaacga
     2941 cgcacgacga cgcgtcagat cataaccgtt tcagtcaacg acctcgactc ttttggtcag
     3001 ggcgtggcgc gacataacgg caaaacgtta tttatccccg gattattgcc gcaggaaaac
     3061 gcggaagttg ctgttactga agataaaaaa cagtatgccc gcgctaaagt cgtacgccgg
     3121 ttaagcgata gcccggaacg cgaaacgcca cgctgtcctc attttggcgt atgcggcggc
     3181 tgtcagcaac aacacgccag cgtggattta cagcagcgaa gcaaaagtgc ggcactcgcc
     3241 cgattaatga aacacgatgt ctctgaagtg atcgccgatg ttccctgggg ctatcgccgc
     3301 cgcgcgcgtt taagtctgaa ctacttaccg aaaacacagc aacttcagat ggggtttcgc
     3361 aaagcgggct ccagtgacat tgtcgacgtc aaacaatgcc ccattttagc gccccaactt
     3421 gaagcattgc tgcccaaagt cagggcatgt ctgggcagct tacaagctat gcgccatctt
     3481 ggtcatgttg aactggtaca ggcaaccagc ggcacgctga tgattttgcg ccataccgca
     3541 ccgctaagtt cggcagatcg cgaaaaactg gaacgctttt cgcattctga aggcctggat
     3601 ctgtatctcg cccccgatag tgagatactc gaaaccgtct ctggtgagat gccctggtat
     3661 gactcaaacg ggttgcgctt aacttttagc ccgcgcgatt ttattcaggt caatgcgggt
     3721 gtgaaccaaa aaatggtagc gcgtgcgttg gaatggctgg atgtgcaacc tgaagatcgc
     3781 gtactggatc tgttctgcgg tatgggcaac tttacactgc cattggcgac acaagctgcc
     3841 agtgtggtcg gtgtagaagg tgttcctgcg ctggtggaaa aaggccagca gaatgcgcgt
     3901 cttaatggct tacagaatgt gacgttttat cacgaaaatc ttgaagaaga tgtcacaaag
     3961 cagccgtggg cgaaaaacgg cttcgataaa gtgttgctgg acccggcgcg agcaggtgcc
     4021 gcaggtgtta tgcagcaaat tataaaactg gaacctattc gtatagttta tgtatcctgt
     4081 aaccctgcaa cgctggctcg ggatagcgaa gcgttattaa aagcaggata taccattgcg
     4141 cgactggcga tgctggatat gttcccacac acgggacatc tggaatcgat ggtacttttc
     4201 tcgcgcgtta aatagttacg atttgccgat ttcggcaggt ctggtcccta aaggagagga
     4261 cgatggttgc ggtaagaagt gcacatatca ataaggctgg tgaatttgat ccggaaaaat
     4321 ggatcgcaag tctgggtatt accagccaga agtcgtgtga gtgcttagcc gaaacctggg
     4381 cgtattgtct gcaacagacg caggggcatc cggatgccag tctgttattg tggcgtggtg
     4441 ttgagatggt ggagatcctc tcgacattaa gtatggacat tgacacgctg cgggcggcgc
     4501 tgctgttccc tctggctgat gccaacgtag tcagcgaaga tgtgctgcgt gagagcgtcg
     4561 gtaagtcggt tgttaacctt attcacggcg tgcgtgatat ggcggcgatc cgccagctga
     4621 aagcgacgca cactgattct gtttcctccg aacaggtcga taacgttcgc cggatgttat
     4681 tggcgatggt cgatgatttt cgctgcgtag tcatcaaact ggcggagcgt attgctcatc
     4741 tgcgcgaagt aaaagatgcg ccggaagatg aacgtgtact ggcggcaaaa gagtgcacca
     4801 atatctacgc gccgttggcg aaccgtcttg ggattgggca actgaaatgg gagctggaag
     4861 attactgctt ccgttatctc cacccgaccg aatacaaacg catcgcaaaa ctgctgcatg
     4921 aacgccgtct cgaccgcgaa cactatatcg aagagtttgt cggccatctg cgcgctgaga
     4981 tgaaagctga aggcgttaaa gctgaagtgt atggtcgtcc gaaacacatc tacagcatct
     5041 ggcgcaaaat gcagaaaaag aacctcgcct tcgatgagct gtttgatgtg cgtgcggtac
     5101 gtattgtcgc cgagcgttta caggattgtt atgccgcact ggggatagtg cacactcact
     5161 atcgccacct gccggatgag tttgacgatt acgtcgctaa cccgaaacca aacggttatc
     5221 agtctattca taccgtggtt ctggggccgg gtggaaaaac cgttgagatc caaatccgca
     5281 ccaaacagat gcatgaagat gcggagttgg gtgttgctgc gcactggaaa tataaagagg
     5341 gcgcggcagc tggcggcgca cgttcgggac atgaagaccg gattgcctgg ctgcgtaaac
     5401 tgattgcgtg gcaggaagag atggctgatt ccggcgaaat gctcgacgaa gtacgtagtc
     5461 aggtgtttga cgaccgggtg tatgtcttta cgccgaaagg tgatgtcgtt gatttgcctg
     5521 cgggatcaac gccgctggac ttcgcttacc acatccacag tgatgtcgga caccgctgca
     5581 tcggggcaaa aattggcggg cgcattgtgc cgttcaccta ccagctgcaa atgggcgacc
     5641 agattgaaat tatcacccag aaacagccga accccagccg tgactggtta aacccaaacc
     5701 tcggttacgt cacaaccagc cgtgggcgtt cgaaaattca cgcctggttc cgtaaacagg
     5761 accgtgacaa aaacattctg gctgggcggc aaatccttga cgacgagctg gaacatctgg
     5821 ggatcagcct gaaagaagca gaaaaacatc tgctgccgcg ttacaacttc aatgatgtcg
     5881 acgagttgct ggcggcgatt ggtggcgggg atatccgtct caatcagatg gtgaacttcc
     5941 tgcaatcgca atttaataag ccgagtgccg aagagcagga cgccgccgcg ctgaaacagc
     6001 ttcagcaaaa aagctacaca ccgcaaaacc gcagtaaaga taacggtcgt gtggtggttg
     6061 aaggtgttgg taacctgatg caccacatcg cgcgctgctg ccagccgatt cctggagatg
     6121 agattgtcgg cttcattacc cagggacgcg gtatttcagt acaccgcgcc gattgcgaac
     6181 aactggcgga actgcgctcc catgcgccag aacgcattgt tgacgcggta tggggtgaga
     6241 gctactccgc cggatattcg ctggtggtcc gcgtggtggc taatgatcgt agtgggttgt
     6301 tacgtgatat cacgaccatt ctcgccaacg agaaggtgaa cgtgcttggc gttgccagcc
     6361 gtagcgacac caaacagcaa ctggcgacca tcgacatgac cattgagatt tacaacctgc
     6421 aagtgctggg gcgcgtgctg ggtaaactca accaggtgcc ggatgttatc gacgcgcgtc
     6481 ggttgcacgg gagttagtct tacatagctg gatatggcgt cagccgtatc cagcgatatc
     6541 ttttactcga aggtattaca cctgacagac ccgcaatttt caggacaatt caatgaatca
     6601 aatcgaccgt ttgctcacta ttatgcagcg cctgcgcgat ccggaaaacg gctgcccgtg
     6661 ggataaagag cagacatttg ccaccattgc gccttacacc cttgaagaaa cctacgaagt
     6721 gctggacgcc atcgcccgtg aagattttga cgatcttcgc ggtgaactgg gcgatctgct
     6781 attccaggtg gtgttttacg cgcaaatggc tcaggaagaa gggcgctttg actttaatga
     6841 tatttgcgct gctattagcg ataaattaga gcgtcgccat ccgcatgttt ttgctgatag
     6901 ttctgccgaa aacagtagtg aagtgcttgc ccgttgggag caaatcaaaa ccgaagagcg
     6961 cgcgcagaaa gcgcagcatt cggcgctgga cgatattcct cgtagtttac cggctttaat
     7021 gcgggcgcaa aaaatccaga aacgttgcgc caacgttggc ttcgactgga cgacgcttgg
     7081 cccggtggtt gataaagtct acgaagagat cgacgaggtg atgtacgagg cgcggcaggc
     7141 tgttgtcgac caggctaaac tggaggagga aatgggggac ctgctgtttg ccacggttaa
     7201 tctggctcgc catttaggga cgaaagcaga aatcgcattg caaaaagtga acgaaaaatt
     7261 cgagcgtcgt tttcgcgaag tggagcgtat tgttgccgcg cgtggactgg aaatgacagg
     7321 tgttgacctc gaaacaatgg aagaagtctg gcaacaggta aaacggcagg aaattgatct
     7381 ctaagggaat tacgcggtca agcgccattt gtgtcatttt ttaaatgaca agcgcttgat
     7441 ttgcgtcaaa aacatttacc ccaaaggggc tattttctca ctcctgattt caatagtgcg
     7501 ctggcgaaga ggagggataa tgaaagtttg tggcacaggt catgttcggg tatactgctt
     7561 tcccgtcttg gttattccat cgtcttttca acctaacttc tcaggttcag catgacaacg
     7621 aactatattt ttgtgaccgg cggggtcgta tcctctctgg gtaaaggcat tgccgcagcc
     7681 tccctcgcag ccattcttga agcccgtggc ctcaatgtga ccatcatgaa actggatcct
     7741 tacatcaacg tcgatccagg tactatgagc ccaatccaac acggggaagt gttcgttact
     7801 gaagacggcg ctgaaaccga cttggacctg gggcactacg agcgtttcat tcgtaccaaa
     7861 atgagccgcc gcaacaactt caccacgggt cgtatctact ctgacgttct gcgtaaagaa
     7921 cgccgcggtg actacctcgg cgcaaccgtg caggttattc cgcacatcac taacgcaatc
     7981 aaagagcgcg tgctggaagg tggcgaaggt catgacgtag tactggtaga aatcggcggt
     8041 acagtaggtg atatcgaatc cttgccgttc ctcgaagcta ttcgccagat ggctgttgaa
     8101 attggccgtg agcacactct gtttatgcac ctgacgctgg tgccgtacat ggcagcgtct
     8161 ggtgaagtca aaaccaaacc gactcagcac tctgtaaaag agctgctctc catcggtatc
     8221 cagcctgaca tcctgatttg tcgttcagat cgcgctgtac cggcgaacga acgtgcgaag
     8281 attgcattgt tctgtaatgt tccggaaaaa gcggttattt ctctgaaaga cgtcgattcc
     8341 atctataaaa ttccgggcct gttgaaatct caggggctgg acgattatat ttgtaaacga
     8401 ttcagcttaa actgcccgga agcaaatctg tccgaatggg aacaggttat cttcgaagaa
     8461 gcgaatccgg taagtgaagt caccatcggt atggtcggca agtacattga actgccggat
     8521 gcttataaat cagtgatcga agcactgaaa cacggtgggc tgaagaatcg tgtcagcgtc
     8581 aacatcaaac tgatcgattc acaagatgtt gaaacgcgcg gcgttgaaat ccttaaaggt
     8641 ctggacgcaa tcctcgtacc tggcggtttc ggctatcgtg gcgtagaagg catgattacg
     8701 accgcgcgtt ttgcgcgtga gaacaatatt ccttatctgg gcatttgcct gggtatgcag
     8761 gtggcgttaa ttgattacgc tcgccatgtt gccaacatgg agaacgccaa ctctacagaa
     8821 tttgtgccag actgtaagta cccggttgtg gcgctgatta ccgagtggcg cgatgaaaac
     8881 ggcaacgttg aagttcgtag cgagaagagc gatctcggcg gtaccatgcg tctcggcgca
     8941 cagcagtgcc agttggttga cgatagcctg gttcgccagc tatacaatgc gccgacaatt
     9001 gttgagcgtc atcgtcaccg ttacgaagtc aacaacatgc tgttgaaaca gattgaagat
     9061 gcaggtctgc gcgttgcggg ccgttccggg gatgatcagt tggtcgagat catcgaagtt
     9121 ccgaatcacc cgtggttcgt ggcttgccag ttccatccgg agtttacttc tactccacgt
     9181 gatggtcacc cgctgtttgc aggctttgtg aaagccgcca gcgagttcca gaaacgtcag
     9241 gcgaagtaag taaaaaagtt agaacggcaa cgcgtacctt gggtacgcgt tgtttgtctg
     9301 gagtttcagt ttaactagtg acttgaggaa aacctaatgt ccaaaatcgt aaaaatcatc
     9361 ggtcgtgaaa tcatcgactc ccgtggtaac ccgactgttg aagccgaagt acatctggag
     9421 ggtggtttcg tcggtatggc agctgctccg tcaggtgctt ctactggttc ccgtgaagct
     9481 ctggaactgc gcgatggcga caaatcccgt ttcctgggta aaggcgtaac caaagctgtt
     9541 gctgcggtaa acggcccgat cgctcaggcg ctgattggca aagatgctaa agatcaggct
     9601 ggcattgaca agatcatgat cgacctggac ggcaccgaaa acaaatccaa attcggcgcg
     9661 aacgcaatcc tggctgtatc tctggctaac gccaaagctg ctgcagctgc taaaggtatg
     9721 ccgctgtacg agcacatcgc tgaactgaac ggcactccgg gcaaatactc catgccggtt
     9781 ccgatgatga acatcatcaa cggtggtgag cacgctgaca acaacgttga tatccaggaa
     9841 ttcatgattc agccggttgg cgcgaaaact gtgaaagaag ccatccgcat gggttctgaa
     9901 gttttccatc acctggcaaa agttctgaaa gcgaaaggca tgaacactgc tgttggtgac
     9961 gaaggtggct atgcgccgaa cctgggttcc aacgctgaag ctctggctgt tatcgctgaa
    10021 gctgttaaag ctgctggtta tgaactgggc aaagacatca ctctggcgat ggactgcgca
    10081 gcttctgaat tctacaaaga tggtaaatac gttctggctg gcgaaggcaa caaagcgttc
    10141 acctctgaag aattcactca cttcctggaa gaactgacca aacagtaccc gatcgtttct
    10201 atcgaagacg gtctggacga atctgactgg gacggtttcg cataccagac caaagttctg
    10261 ggcgacaaaa tccagctggt tggtgacgac ctgttcgtaa ccaacaccaa gatcctgaaa
    10321 gaaggtatcg aaaaaggtat cgctaactcc atcctgatca aattcaacca gatcggttct
    10381 ctgaccgaaa ctctggctgc aatcaagatg gcgaaagatg ctggctacac tgcagttatc
    10441 tctcaccgtt ctggtgaaac tgaagatgct accatcgctg atctggctgt tggtactgct
    10501 gcaggccaga tcaaaactgg ttctatgagc cgttctgacc gtgttgctaa atacaaccag
    10561 ctgattcgta tcgaagaagc tctgggcgaa aaagcaccgt acaacggtcg taaagagatc
    10621 aaaggccagg cataagtctg actttatctg atttgataat gccagccttc gggctggcat
    10681 tttttatggt ggagttacca gcggccggat gcgccgccac ttgagcctcc agcggaaccg
    10741 ctgttgtctg aatgataact tctaccagat gctcttttct gaagtacaca taaacaggta
    10801 aagaccataa atatggtgaa agtaaaaaag aaggaaaata cagcaatgga aaaagtttta
    10861 tcggagaaca ggaataaatc taaaagatgg attgctgaaa tacagactcc acttttacat
    10921 gcgcgacaaa aattactgcc ttgatgaaaa aaccagaaag ggaagaacat cactgcaaat
    10981 acccaaaaaa tagcaaagta ataatgatca gtggaagacg ttgattcact ttctgaagga
    11041 ttagccggat attggtgttg tgaagtgagt tggttattca aagcgattat agctaactct
    11101 aatcccttag ctaatttttg ttgtttaaat gcgggtatca tgttgctacg gatgatatct
    11161 ccagccagag catcggttac cttttcttcc agcccataac ctacctggat gcggacagtg
    11221 cgatccgact aggcaacaac gatcagtatc ccatcattac gtttggcatc tcctaaacgc
    11281 caattgtcaa aaactcttgt agcatattgt tcaattgtct catctttggt tgtggaaaca
    11341 attaatacag caatttgcac ttgggtatta ttgaaaatag attctaactt gtgcgtcagt
    11401 tctttctttt cttgtgagtt taagctactc gtaagacccg taacttgttg ctgtaattgt
    11461 gggacaatag ttggttgggc tgcaacaa
//
LOCUS       DAZPFP010000116        11457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPFP010000116 DAZPFP010000000
VERSION     DAZPFP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.116"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            42..314
                     /gene="rcnR"
                     /locus_tag="ACHUR4_002856"
     CDS             42..314
                     /gene="rcnR"
                     /locus_tag="ACHUR4_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1456366.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      406..502
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            537..1325
                     /gene="thiM"
                     /locus_tag="ACHUR4_002857"
     CDS             537..1325
                     /gene="thiM"
                     /locus_tag="ACHUR4_002857"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1456367.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            1322..2122
                     /gene="thiD"
                     /locus_tag="ACHUR4_002858"
     CDS             1322..2122
                     /gene="thiD"
                     /locus_tag="ACHUR4_002858"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1456368.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            2187..3004
                     /locus_tag="ACHUR4_002859"
                     /pseudo
     CDS             2187..3004
                     /locus_tag="ACHUR4_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            3056..3802
                     /locus_tag="ACHUR4_002860"
     CDS             3056..3802
                     /locus_tag="ACHUR4_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1456369.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(3776..4741)
                     /locus_tag="ACHUR4_002861"
     CDS             complement(3776..4741)
                     /locus_tag="ACHUR4_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1456370.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4738..5741)
                     /locus_tag="ACHUR4_002862"
                     /pseudo
     CDS             complement(4738..5741)
                     /locus_tag="ACHUR4_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            5781..6833
                     /gene="fbaB"
                     /locus_tag="ACHUR4_002863"
     CDS             5781..6833
                     /gene="fbaB"
                     /locus_tag="ACHUR4_002863"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1456371.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            7141..7995
                     /gene="gatY"
                     /locus_tag="ACHUR4_002864"
     CDS             7141..7995
                     /gene="gatY"
                     /locus_tag="ACHUR4_002864"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1456372.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            8024..9286
                     /gene="gatZ"
                     /locus_tag="ACHUR4_002865"
     CDS             8024..9286
                     /gene="gatZ"
                     /locus_tag="ACHUR4_002865"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR1456373.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            9296..9748
                     /gene="gatA"
                     /locus_tag="ACHUR4_002866"
     CDS             9296..9748
                     /gene="gatA"
                     /locus_tag="ACHUR4_002866"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1456374.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            9779..10063
                     /gene="gatB"
                     /locus_tag="ACHUR4_002867"
     CDS             9779..10063
                     /gene="gatB"
                     /locus_tag="ACHUR4_002867"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1456375.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            10067..11422
                     /locus_tag="ACHUR4_002868"
     CDS             10067..11422
                     /locus_tag="ACHUR4_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1456376.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         2769 a         2809 c         3040 g         2839 t
ORIGIN      
        1 agtgctatga tttttcatat tcttgttaac caggtgttgc catgtctcat acaatccgtg
       61 ataaacagaa actgaaagcg cgtgccagta agattcaggg ccaggtcgtg gcgctcaaga
      121 aaatgctcga cgagccgcac gaatgcgctg cagttttaca acagattgct gctatccgtg
      181 gagcggtaaa cggtctgatg cgggaagtga ttaaaggtca tctgacggaa cacatcgttc
      241 accaggggga tgagctaaaa cgtgaagaag atctggatgt cgttctgaag gtgctggatt
      301 catatatcaa ataatttatt aacgcgattg taaaactgcc gtttttcctc gtttacaacg
      361 ggggcgctgg acattaccat cctcctctgc gatttatcat cgcaaccaaa cgactcgggg
      421 tgcccttctg cgtgaaggct gagaaatacc cgtatcacct gatctggata atgccagcgt
      481 agggaagtca cggaccacca ggtcattgct tcttcacgtt atggcaggag caaactatgc
      541 aagtcgacct gctgagttca gcgcaatctg cgcacgcgtt acaccttttt caccaacatt
      601 cccctcttgt gcactgcatg accaatgatg tggtgcaaac ctttaccgcc aataccttgc
      661 tggcgctcgg tgcatcgcca gcgatggtta tcgaaaccga agaggccagt cagtttgcgg
      721 ctatcgccag tgccttgttg attaacgttg gcacactgac gcagccacgc gcacaggcga
      781 tgcgtgctgc cgttgagcaa gcaaaaagct ctcaaacacg ctggacgctt gatccagtag
      841 cggttggtgc gctcgattat cgccgccatt tttgtcatga acttttatct tttaaaccgg
      901 cagcgatacg tggtaatgct tcggaaatca tggcattagc tggcattgct aatggcggac
      961 ggggagtgga taccactgac gccgcagcta acgcgatacc cgctgcacaa acactggcac
     1021 gggaaactgg cgcaatcgtc gtggtcactg gcgaggtgga ttatgttacc gatggacatc
     1081 gtatcattgg tattcacggt ggcgatccgt taatgaccaa agtggtagga actggctgtg
     1141 cattatcggc ggttgtcgct gcctgctgtg cgttaccagg cgatacgctg gaaaatgtcg
     1201 catctgcctg tcactggatg aaacaagccg gagaacgcgc agtcgccaga agcgaggggc
     1261 caggcagttt tgttccacat ttccttgatg cgctctggca attgacgcag gaggtgcagg
     1321 catgaaacga attaacgctc tgacgattgc cggtactgat ccgagtggtg gtgcggggat
     1381 tcaggccgat cttaaaacct tctcggcact tggcgcttat ggttgctcag ttattactgc
     1441 actggtggcg caaaataccc gtggcgtaca gtcggtgtat cgcattgagc ctgattttgt
     1501 cgccgcccag ctcgattcgg tgttcagcga tgtgcgaatc gataccacta aaatcggtat
     1561 gttggcggaa accgatattg ttgaagcggt ggcagaacgg ttgcaacgtt atcagatcca
     1621 aaacgtggta ctcgacaccg ttatgctggc aaaaagcggc gacccgctgc tttcaccttc
     1681 ggcggttgct acgctgcgca gtcgattatt gccacaggtt tcattaataa cgccaaactt
     1741 gcccgaagct gccgccttgc tcgacgcgcc acacgcgcgc accgaacagg aaatgctgga
     1801 acaagggcga tcgctgttgg cgatgggctg tggcgcagtg ctaatgaaag gtggtcatct
     1861 ggatgatgag caaagcccgg actggctgtt tacccgcgag ggtgaacaac ggtttaccgc
     1921 accgcgcatt atgaccaaaa acacccacgg cactggttgt acactctctg cggcgttggc
     1981 tgcactacgc ccgcgccata caaactgggc tgacaccgta caggaggcaa aaatctggct
     2041 ttcatcggcg ttagcccagg ccgacacgct ggaagttggt cacggtattg gtccggttca
     2101 ccacttccac gcctggtggt gagcaccgta ctatagctgc tttggttatt gtcagccttt
     2161 ggcactgaca ttcagggatg aaaccaatgc agctaagaat taccagtcga aaaaaactca
     2221 ccgctctatt atgcgctctt gggctaattt ccatcgttgc aatttatccg cgtcagacgg
     2281 tgaatttttt ctattcgaca gcagttcaga ttacagacta catccacttc tacggttatc
     2341 gcccggttaa atctttcgct attcgcattc ctgccagtta caccattcac ggattagatg
     2401 tttcgcgctg gcaggagcgt atcgactggc agcgggtggc aaaaatgcgc gacaacgata
     2461 tccgcttaca gtttgctttt attaaggcta cggaaggcga aaagctggtg gacccctatt
     2521 tttcgcgtaa ctggcaatta agccgcgaaa atggcctgct gcgcggggcg tatcattatt
     2581 tctccccgtc ggtatccgct tcagttcagg cgcgattatt tctgcaaacg gtggatttct
     2641 cacaaggcga tttccctgcc gtgctggacg tagaagaacg gggaaaatta tcggcaaaag
     2701 aattacgcaa gcgggtaagt cagtggctaa aaatggtcga aaaacgcacg ggaaaaaagc
     2761 cgattattta ctcaggagcc gttttttatc acaccaatct ggcgggctat ttcaatgaat
     2821 atccgtggtg ggtggctcac tattatcaac gtcgtccgga caatgacggc atggcctggc
     2881 gcttctggca gcattccgac cgtggacagg tagatggcat caatggtccg gtggatttta
     2941 atgtgtttaa tggcacgggg atgagttgca ggcattcgtt gatgggatta aagaaacgcc
     3001 ttaaataaga agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga
     3061 acaagcgcat acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac
     3121 gccgctttat attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga
     3181 gcatggcaat attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat
     3241 tacggtgcgc aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg
     3301 ttacggcacg cagatcaaca acatcttcga atactcgttg aaagaagcga gaggtttttc
     3361 tcagcaggtg gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt
     3421 gaaatgtccc gaagaagttg cgcagcagct ggcggccgaa gcgggaagtg atgtcttttt
     3481 gcttaagcgt attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc
     3541 ggcgcattta attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag
     3601 ccagcacatt tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga
     3661 gtttcagtca catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc
     3721 gcttgaccaa cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata
     3781 cgtttttgtg tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc
     3841 cgacaaccca cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag
     3901 aggccagccc ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa
     3961 cttgcgttgg aaatgccgga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt
     4021 ctttatcgag acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg
     4081 ttgttatctc tgcggataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga
     4141 gtgacactaa aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa
     4201 tacgtgggca aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta
     4261 acaattcgcc gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg
     4321 caacggttaa tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca
     4381 taaaagtgcg ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg
     4441 cgttatcgat caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc
     4501 acacgccctg accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca
     4561 cggcaatatt cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc
     4621 acccacgcca ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga
     4681 ccatcaccgg ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca
     4741 taccgcctcc cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag
     4801 ctgatttacc gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa
     4861 cgcgccgcaa atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc
     4921 gcacaggacg gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca
     4981 cggaacggac tcaatagtgc tggtgcctgc gccaacgacc tggtaaagct gttcgctggc
     5041 ggattcggtg ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag
     5101 tgaggcgctg aacgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga
     5161 atcaacaatc gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac
     5221 taccgcgcct gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc
     5281 atcaataaag gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat
     5341 cgctgcgccg ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat
     5401 ggcgttaagc gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg
     5461 caacgcccag tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc
     5521 cagtaacgca tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta
     5581 aaataacagg ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag
     5641 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
     5701 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgaatcg
     5761 gaatttacgg gagcatagta atgacagata ttgcgcagtt gcttggcaaa gacgccgaca
     5821 accttttaca gcaccgttgt atgactattc cttctgacca gctttatctc cccggacatg
     5881 actacgtaga ccgcgtgatg attgacaata atcgcccgcc agcggtgtta cgtaatatgc
     5941 agacgttgta caacaccggg cgtctggctg gcacaggata tctttctatt ctgccggttg
     6001 accagggcgt tgagcactct gccggagctt catttgctgc taacccgctc tactttgacc
     6061 cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg
     6121 gcgtgttggt gtcggtatcg cggcgctatg cgcatcgcat tccatttctc gtcaaactta
     6181 atcacaacga gacgctaagt tacccgaata cctacgatca aacgctgtat gccagcgtgg
     6241 agcaggcctt caacatgggc gcggtggcgg ttggtgcgac tatctatttt ggctcggaag
     6301 agtcacgtcg ccagattgaa gaaatttctg cggcctttga acgcgcgcac gagctgggca
     6361 tggtgacagt gctgtgggcc tatttgcgaa actccgcctt taagaaagat ggcgttgatt
     6421 accatgtttc cgccgacctg actggtcagg cgaaccatct ggcggcaacc ataggtgcag
     6481 atatcgtcaa acaaaaaatg gcggaaaata acggcggcta taaagcgatt aattacggtt
     6541 ataccgacga tcgcgtgtac agcaagttaa ccagcgaaaa cccgattgat ctggtgcgtt
     6601 atcagttagc taactgctat atgggccggg ctgggttgat aaactccggc ggtgctgcag
     6661 gcggtgaaac tgacctcagc gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg
     6721 gaatggggct gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac
     6781 tgattaatgc cgtgcaggac gtttatctcg atagcaaaat tactattgcc tgatacgcct
     6841 catacgaaca gcaccacccc gtaggacgga taagacgttt acactgcatc cggcaaccta
     6901 tgcctgatgc gacgcggaag cgtcttatca tgcctacata gcactgccac gtatgtttac
     6961 accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt
     7021 ttttgccttt cactcctcga acaattttca tattgttgtt tttgcgatca ttatctcgac
     7081 atttaaaagc aaataatttc attatatttt gaaatcgaaa gcaaacgaca agatatgaaa
     7141 atgtacgtgg tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt
     7201 ccggcattca atattcacaa tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac
     7261 ctgcatgcgc cggtcatcat cgccggaacg cctggcacat ttactcatgc tggtacagaa
     7321 aatctgttgg cgctggtcag cgcgatggcg aagcaatatc accatccact ggcaattcat
     7381 ctcgaccatc acacgaaatt tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca
     7441 gtcatgattg acgcctcgca tttgcctttt gcgcaaaata tttcacgggt caaagaggtg
     7501 gtggattttt gccatcgctt tgatgtcagc gtcgaggcgg agctggggca acttggcggc
     7561 caggaagatg atgtgcaagt caatgaagtc gatgcgttgt acaccaaccc cgcacaggcg
     7621 cgtgaatttg ccgaggcaac cgggattgat tccctggcgg tcgccatcgg cacggctcac
     7681 ggtatgtatg ccagcgcacc ggtgcttgat ttttctcgac tggagaacat tcgccagtgg
     7741 gtgaacttac cgctggtgct gcatggcgcg tcaggattat cgactaagga tattcagcaa
     7801 accatcaaac tggggatatg caaaatcaac gttgcaacgg agctgaaaaa tgccttcttg
     7861 cagtcgttaa aaaattacct gaccgagcac cccgaagcga ccgatccccg ggattatttg
     7921 cagtcggcta aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc
     7981 gagggcaggg cataacgcac ctgccattta acaaggaaaa aacatgaaaa cgttaattgc
     8041 ccggcataaa gctggtgaac atatcggcat atgttcagtc tgttctgccc atccgttggt
     8101 tatcgaagcg gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac
     8161 gtcaaaccag gtcaatcaat ttggcggtta taccggaatg acaccggcag actttcgcga
     8221 atttgttttt acgattgccg ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg
     8281 cgatcatctg ggaccaaact gctggcagca agaaaatgcg gatgcggcga tggaaaaatc
     8341 cgtcgagctg gtaaaagcat atgttcgtgc tggcttcagt aaaattcatc ttgatgcgtc
     8401 aatgtcctgc gcgggggata ccataccgtt agcaccagaa acggttgcgg aacgagctgc
     8461 tgtgctttgc tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc aactgagcta
     8521 tgtcattggc actgaagttc cggttccggg cggtgaggcc agcgccattc agtcagtaca
     8581 catcacccat gttgaagatg ccgccaatac tttacgtacg catcaaaagg cctttattgc
     8641 ccgtgggctg acagaggcgt taacacgtgt gattgccatc gtggtgcagc cgggtgtgga
     8701 atttgatcac agcaatatta tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg
     8761 gatagaaaac acccgaatgg tttatgaagc acattctacc gattaccaga cccggacggc
     8821 ttattgggaa ttagtccgcg atcactttgc aatattgaaa gtcggtcccg cattaacctt
     8881 tgctttacgc gaggcgatat ttgcactggc acaaattgag caggaactta tcgcccccga
     8941 aaatcgcagc ggttgcctgg cggtaattga agaagtgatg ctcgacgaac cgcaatactg
     9001 gaaaaaatat tatcgcacgg gttttaacga ttcattactg gatattcgtt acagcctgtc
     9061 ggatcgtatt cgttattact ggccgcatag tcggattaaa aatagcgttg aaacgatgat
     9121 ggtgaatctg caaggcgtgg acatcccact gggcatgatt agtcagtatc ttcccaaaca
     9181 atttgaacgc attcagtccg gggaattatc agcaatgccg catcagctta ttatgaataa
     9241 aatttatgat gttttgcgcg cctatcgcta cggctgtgcg gaataaggac ggtatatgac
     9301 taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt taacctatat
     9361 cggtaatgag atgctcgcga aaggtgtggt tcatgatacc tggccacagg cattaattgc
     9421 cagagaagca gaattcccta ccgggataat gcttgagcag cacgccattg caataccgca
     9481 ttgtgaggcg attcatgcta agtcgtcagc catttatctg ttaaggccaa caaataaagt
     9541 tcattttcag caagcggatg atgataacga cgtggcggta tcgttggtta ttgcgttgat
     9601 tgtggaaaat ccgcagcagc aattgaaact tttacgctgt ttatttggca agttacaaca
     9661 gcccgatatc gtcgagacac taattactct tcctgaaact cagttaaagg aatacttcac
     9721 aaagtatgtt ttagattcag acgaataaat ccctctgtaa caataaaaag gactatttat
     9781 gaaacgcaag attattgtcg cttgcggagg cgcggttgcg acctctacga tggcggcgga
     9841 agaaattaaa gagttgtgtc agagtcataa tattcctgtt gaattaatcc agtgtcgggt
     9901 taatgaaata gaaacctata tggatggtgt gcatttgata tgtaccactg ccagggtgga
     9961 tcgtagtttt ggcgatattc cgttagttca cggcatgcct tttgtttctg gtgtcggtat
    10021 cgaagcatta caaaataaaa ttctgactat cttacaggga tgacctatgt tttcagaagt
    10081 catgcgttat attctcgacc tcggccctac agtgatgctg ccggttgtca tcattatttt
    10141 ttctaaaata ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc atatcgggat
    10201 tggctttgtt ggcattggcc ttgtgattgg cttaatgctg gattccattg gtccggcggc
    10261 gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg
    10321 ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt
    10381 ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg atatctggaa
    10441 tatctggcat atgaccttca ccggcgcgtt gctgcacctg gcaaccggtt catggatgat
    10501 agggatggcg ggtgtggtaa ttcacgcggc gtttgtttat aagctcggcg actggtttgc
    10561 ccgcgatacc cgaaatttct ttgagctgga aggtattgct attccgcacg gtacgtcggc
    10621 gtatatgggg ccgattgcgg tgctggtcga tgctatcatc gagaaaatcc caggcgttaa
    10681 ccgaattaaa tttagcgccg acgatattca gcgcaaattt ggcccttttg gcgaacctgt
    10741 caccgtgggt tttgtgatgg ggctgattat cggcatcctc gcgggttacg atgtcaaagg
    10801 tgtattgcag ctggcggtaa aaacggcggc agttatgctg ctgatgccac ggatgattaa
    10861 acccatcatg gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa
    10921 gttcggcggt caggagttcc tgattggcct ggatcccgca ttactgctgg ggcatacggc
    10981 ggtggtatcg gcaagcctga tttttattcc gctcaccatt ttaattgctg tttgtgttcc
    11041 gggtaatcag gtactgccgt ttggcgatct tgccactatc ggcttcttcg tggcgatggc
    11101 ggtcgcggtg catcgtggaa atctgttccg caccttaatc tcgggtgtca tcattatgag
    11161 catcaccctg tggatcgcga cgcaaactat tggtttgcac acccaaccgg cggctaatgc
    11221 tggggcgtta aaagccgggg gtatggtggc ttcaatggat cagggcggtt ctcccattac
    11281 ctggttactg attcaggttt tctccccgca aaatattccc ggtttcatta ttatcggcgc
    11341 aatttatctg accggtattt tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga
    11401 gaaagccgtt ctcgcagaat aatttttacc tgagggggag gttatccccc ttaacaa
//
LOCUS       DAZPFP010000117        11453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPFP010000117 DAZPFP010000000
VERSION     DAZPFP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.117"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHUR4_002869"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHUR4_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUR4_002870"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUR4_002870"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1456377.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUR4_002871"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUR4_002871"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR1456378.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUR4_002872"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUR4_002872"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR1456379.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUR4_002873"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUR4_002873"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR1456380.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUR4_002874"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUR4_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR1456381.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHUR4_002875"
     CDS             complement(8300..11167)
                     /locus_tag="ACHUR4_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1456382.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2764 a         2818 c         2853 g         3018 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gtt
//
LOCUS       DAZPFP010000118        11325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPFP010000118 DAZPFP010000000
VERSION     DAZPFP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.118"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACHUR4_002876"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACHUR4_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1456383.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /locus_tag="ACHUR4_002877"
     CDS             323..1954
                     /locus_tag="ACHUR4_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1456384.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="ACHUR4_002878"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="ACHUR4_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1456385.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="ACHUR4_002879"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="ACHUR4_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1456386.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="ACHUR4_002880"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="ACHUR4_002880"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1456387.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="ACHUR4_002881"
     CDS             6428..7057
                     /locus_tag="ACHUR4_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1456388.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACHUR4_002882"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACHUR4_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1456389.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACHUR4_002883"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACHUR4_002883"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1456390.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="ACHUR4_002884"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="ACHUR4_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1456391.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2963 a         2912 c         2749 g         2701 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaagc tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaat
//
LOCUS       DAZPFP010000119        11291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPFP010000119 DAZPFP010000000
VERSION     DAZPFP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.119"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            107..241
                     /locus_tag="ACHUR4_002885"
     CDS             107..241
                     /locus_tag="ACHUR4_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1456392.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            220..324
                     /gene="speFL"
                     /locus_tag="ACHUR4_002886"
     CDS             220..324
                     /gene="speFL"
                     /locus_tag="ACHUR4_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1456393.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            635..2832
                     /gene="speF"
                     /locus_tag="ACHUR4_002887"
                     /pseudo
     CDS             635..2832
                     /gene="speF"
                     /locus_tag="ACHUR4_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2829..4148
                     /gene="potE"
                     /locus_tag="ACHUR4_002888"
     CDS             2829..4148
                     /gene="potE"
                     /locus_tag="ACHUR4_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1456394.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4204..5844)
                     /gene="pgm"
                     /locus_tag="ACHUR4_002889"
     CDS             complement(4204..5844)
                     /gene="pgm"
                     /locus_tag="ACHUR4_002889"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1456395.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5870..6415)
                     /gene="seqA"
                     /locus_tag="ACHUR4_002890"
     CDS             complement(5870..6415)
                     /gene="seqA"
                     /locus_tag="ACHUR4_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1456396.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6600..7364
                     /gene="ybfF"
                     /locus_tag="ACHUR4_002891"
     CDS             6600..7364
                     /gene="ybfF"
                     /locus_tag="ACHUR4_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1456397.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7504..7797
                     /gene="ybfE"
                     /locus_tag="ACHUR4_002892"
     CDS             7504..7797
                     /gene="ybfE"
                     /locus_tag="ACHUR4_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1456398.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7937..8467
                     /gene="fldA"
                     /locus_tag="ACHUR4_002893"
     CDS             7937..8467
                     /gene="fldA"
                     /locus_tag="ACHUR4_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1456399.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8677..8763
                     /gene="uof"
                     /locus_tag="ACHUR4_002894"
     CDS             8677..8763
                     /gene="uof"
                     /locus_tag="ACHUR4_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1456400.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8756..9202
                     /gene="fur"
                     /locus_tag="ACHUR4_002895"
     CDS             8756..9202
                     /gene="fur"
                     /locus_tag="ACHUR4_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1456401.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9286..9612)
                     /gene="chiQ"
                     /locus_tag="ACHUR4_002896"
     CDS             complement(9286..9612)
                     /gene="chiQ"
                     /locus_tag="ACHUR4_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1456402.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9649..11055)
                     /gene="chiP"
                     /locus_tag="ACHUR4_002897"
     CDS             complement(9649..11055)
                     /gene="chiP"
                     /locus_tag="ACHUR4_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR1456403.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2761 a         2776 c         2804 g         2950 t
ORIGIN      
        1 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
       61 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
      121 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
      181 aactctacct tcaactgaca cagcaagcgg taaaggtaaa tggaaaataa taaccgctta
      241 atgcctcata taaggcggac aacccatatc atgaagttta cccatcgtaa tagttttgac
      301 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
      361 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
      421 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
      481 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
      541 tcagcggtgg agctttgctt tttttccgac gtgatcgatt tctcctttga gaaattgagg
      601 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
      661 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
      721 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
      781 taaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
      841 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
      901 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
      961 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     1021 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     1081 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     1141 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     1201 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     1261 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     1321 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
     1381 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
     1441 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
     1501 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
     1561 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
     1621 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctagt
     1681 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
     1741 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
     1801 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
     1861 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
     1921 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
     1981 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
     2041 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
     2101 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
     2161 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
     2221 agtgccggaa aaatgcgatc ttaacgccat cctcttcctg ctgactccgg cagaagatat
     2281 ggccaaactt cagcaacttg tgccctgctg gtacgcttcg aaaaactgct tgagtccgac
     2341 gcgccattag cagaagtgct gccttccatc tacaaacagc atgaagagcg ctacgccggt
     2401 tataccctgc gtcagttgtg ccaggaaatg catgatttgt atgcccgcca caacgtgaaa
     2461 caactgcaaa aagagatgtt ccgcaaagcg cacttcccac acgtcagcat gaatccgcaa
     2521 gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctacc ggatgccgaa
     2581 ggtcgtatcg ctgccgaagg ggcgcttcct tatcctccgg gtgtgctgtg tgttgttccg
     2641 ggtgaaatct ggggtggtgc tgttttgcgt tacttcagcg ctctggaaga agggatcaac
     2701 ctgctgccag gttttgcacc ggagctgcag ggtgtctata tcgaagaaca tgatggtcgt
     2761 aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg
     2821 gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga
     2881 cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg
     2941 ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg
     3001 cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg tatgggcggt tatgccgagt
     3061 atgctttcgg taaatccggt aactttattg cgaactatac ctacggcgtc tcactgctga
     3121 ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga
     3181 gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt tgtaccgtgg
     3241 ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg tgggggggca
     3301 ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg
     3361 ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta
     3421 tgacgctgtg ggcttttctt ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa
     3481 acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt
     3541 atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa
     3601 cggcaccatt tggcctggcc ttcgcacaga tgtttacacc agaagtgggt aaagtcatta
     3661 tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc
     3721 aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca
     3781 aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc
     3841 tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg
     3901 tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg
     3961 ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt
     4021 atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtga
     4081 ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac
     4141 acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgctcttttt ttattaaatg
     4201 tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt
     4261 gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
     4321 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccaa tagaagcacc
     4381 gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca cctgccaggg tgctggcgct
     4441 caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagctgcctg
     4501 caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg
     4561 gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt
     4621 gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
     4681 gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac
     4741 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag aagaaaccag
     4801 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat
     4861 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
     4921 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcagacc
     4981 cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat cgaggtgcat
     5041 aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc
     5101 gccaatgcgc ttccagtatt cgataccgga accgcccaac ggatcaacgc ccagcgtcag
     5161 gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca cgaacggctg
     5221 caccagatcc tgctctttca catagccgga tgccatcgct tcgtcgaggg agatacgctt
     5281 cacgcctttc aggccatcgg ccatcagtgc gttggccttg tcttccacca ctttggtgac
     5341 gttagtatca gccgggccac catttggcgg attgtacttg ataccaccat cttccggcgg
     5401 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
     5461 caggatggca ttggaaacgg caggcgtcgg agtgaagcca ttgttttcct gcacaatgac
     5521 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc
     5581 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
     5641 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
     5701 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
     5761 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
     5821 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
     5881 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
     5941 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat
     6001 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaca
     6061 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
     6121 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
     6181 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
     6241 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
     6301 atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
     6361 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
     6421 tccagtgcag tgtcttagct gaatacaagt cgccaagagt atatgaataa atcccggcag
     6481 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
     6541 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
     6601 tgaaattaaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
     6661 ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa
     6721 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg
     6781 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg
     6841 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac
     6901 tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg
     6961 tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa
     7021 ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
     7081 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc
     7141 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta
     7201 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
     7261 ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
     7321 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
     7381 cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat
     7441 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
     7501 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
     7561 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
     7621 aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
     7681 gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta atatgagccg tagcgaactg
     7741 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
     7801 tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
     7861 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
     7921 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
     7981 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
     8041 cattgcaaaa agcagcaaag aagatctgga agcttacgac attctgctgc tgggcatccc
     8101 aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
     8161 gattgatttc aatggtaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc
     8221 cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac
     8281 catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga
     8341 tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga
     8401 acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg aaattcttaa
     8461 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
     8521 aaataattgc tacaaatttg taacttttgc tgttgtacct atacaatgtc ccagtgttca
     8581 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
     8641 gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
     8701 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
     8761 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat
     8821 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
     8881 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
     8941 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
     9001 gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt
     9061 tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct
     9121 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
     9181 gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg cgggttggct
     9241 tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact
     9301 gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg
     9361 cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt cggcaaactg
     9421 ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc
     9481 tggcgaacct tgcgcggtgt tgatacaggc gctgtatgcc tctttcagac ggctgtcttc
     9541 ctgtggtgcg gttgattgcg cacaagctac cagccccgat gccatcatgg cgatgagaat
     9601 cagttttttc attgaatgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     9661 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc
     9721 gccgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     9781 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc
     9841 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
     9901 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     9961 caggtcatac atcgcactga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    10021 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttgggg tcatacgttg
    10081 caggaagtat ccctgctgac tgtcagcctt aatccaggtg ccttcgaggc gcaaatccac
    10141 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    10201 atcgttgacg ctgcgatcgt caactttatc gcgggtacca tagaactggt agctggtggt
    10261 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    10321 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    10381 accgatggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    10441 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc
    10501 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ccggcataaa
    10561 gctccagtgc ggcgctaaca gcgtttgacc ggttggctga atgtaacctg ccctcgccca
    10621 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    10681 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    10741 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    10801 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    10861 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    10921 gataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    10981 cataaagccc gacattgctg taacaccggc aatagccagc gccagcgtac tacgtttgcc
    11041 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    11101 agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg actacaggaa
    11161 cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat gtaagaacat
    11221 gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg gttgaaaggc
    11281 ttgcagaaga a
//
LOCUS       DAZPFP010000120        11225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPFP010000120 DAZPFP010000000
VERSION     DAZPFP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.120"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            2..1116
                     /gene="yghO"
                     /locus_tag="ACHUR4_002898"
                     /pseudo
     CDS             2..1116
                     /gene="yghO"
                     /locus_tag="ACHUR4_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1240..2010)
                     /gene="glcC"
                     /locus_tag="ACHUR4_002899"
     CDS             complement(1240..2010)
                     /gene="glcC"
                     /locus_tag="ACHUR4_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR1456404.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2261..3760
                     /gene="glcD"
                     /locus_tag="ACHUR4_002900"
                     /pseudo
     CDS             2261..3760
                     /gene="glcD"
                     /locus_tag="ACHUR4_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3760..4812
                     /gene="glcE"
                     /locus_tag="ACHUR4_002901"
     CDS             3760..4812
                     /gene="glcE"
                     /locus_tag="ACHUR4_002901"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR1456405.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4823..6042
                     /gene="glcF"
                     /locus_tag="ACHUR4_002902"
                     /pseudo
     CDS             4823..6042
                     /gene="glcF"
                     /locus_tag="ACHUR4_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6047..6451
                     /locus_tag="ACHUR4_002903"
     CDS             6047..6451
                     /locus_tag="ACHUR4_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR1456406.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6473..8644
                     /gene="glcB"
                     /locus_tag="ACHUR4_002904"
     CDS             6473..8644
                     /gene="glcB"
                     /locus_tag="ACHUR4_002904"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR1456407.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8999..10585
                     /locus_tag="ACHUR4_002905"
     CDS             8999..10585
                     /locus_tag="ACHUR4_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR1456408.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2658 a         2767 c         3110 g         2690 t
ORIGIN      
        1 aattgaaaaa gtaattaata aaaatgacct taaggcattt atcgcttttc cgtcatcact
       61 ttatcccgac gatccaaact ggataccccc tttattcatt gaacgcaacg agcatttgtc
      121 tgcgaaaaat ccggggacgg accatatcat ctggcaggcg tgggtggcaa aaaaagcggg
      181 gcagatagtt gggcgtatta ccgcgcaaat agataccttg caccgtgagc gttacggtga
      241 agataccggt catttcggca tgattgacgc cattgatgat ccgcaggatt ttgccgcgtt
      301 gtttggcgca gcggaagcgt ggttgaagtc acaaggtgca agtaagatca gcgggccttt
      361 cagcctgaat atcaatcagg aaagcggatt actgattgaa ggttttgaca caccaccctg
      421 tgcgatgatg ccacacggca aaccgtggta tgccgcgcat attgaacaac tgggttatca
      481 caaaggcatt gatttactgg cgtggtggat gcagcgaacc gatctcactt tctctccggc
      541 gctaaaaaaa ctgatggatc aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca
      601 gcggtttgcc gaagagatgc agatcctgcg tgagattttc aactctggct ggcagcacaa
      661 ctggggattt gtgccgttta ccgaacatga attcgcgacc atgggcgacc aacttaaata
      721 tctggtgccg gacgatatga tctacatcgc tgagattgat tctgcaccct gcgcgtttat
      781 tgtggcttgc ctaacatcaa cgaggcgatt gccgatctga acggatcgct ctttcccttc
      841 ggctgggcaa aattgctgtg gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg
      901 ctgatgggcg tgcgcgacga gtatcagttc agccgcatcg gcccggtgat tgcgctgtta
      961 ttgattgaag ccttacgcga tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca
     1021 tggatcctcg aaaccaatac aggcatgaat aacatgctgg aacgcattgg ggcggagccg
     1081 tacaagcgtt accgattgta tgaaaagcaa atttaatacc agatcctgtt tagcgcaggg
     1141 gcggtcattc gggagagggt atagatgaac agatttaaca ccagatcctg ttaagtgcag
     1201 gggcggtccc ctcgccttgg ggatagggta aaactctgct cacattccca ccttgttcat
     1261 ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt
     1321 ctttttcacg gtccgtacat gatcgcgcgc tgcgcgctga gcgacgtgcg gcagccgctg
     1381 caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg
     1441 atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg
     1501 cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg
     1561 atccaactgc gcatgttcga tcagcgaaat ctctttgtgg ttctcactgg cggcgagcat
     1621 tttttcataa cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag
     1681 ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg
     1741 ctgcgtactg aacagatgga tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac
     1801 atgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc
     1861 ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag
     1921 cggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac
     1981 ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat
     2041 ttttctgttc ttaactcgca aaacacgcac atcacgcaag tgattatgtt acatcaattt
     2101 aacattaagt taaccaagac aaggtcacag agctggaaaa aaaatggtct gaccggtagc
     2161 taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa
     2221 aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2281 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2341 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2401 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2461 ggtgacagcg attctggctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2521 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2581 gcgctttaaa gagagcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg
     2641 cgtatgtaac ctggcgatct cctaggccgt tgcgccgcat aatctctatt acgcgccgga
     2701 tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt
     2761 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     2821 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     2881 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     2941 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     3001 cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     3061 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     3121 cgaagcgatt ttgttatgcg aactggacgg cgtggagtct gacgtacagg aagactgcga
     3181 gcgggttaac tacatcttgt tgaaagcggg cgcgactgac gtccgtttgg cacaggacga
     3241 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3301 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3361 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3421 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc
     3481 ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3541 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3601 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3661 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3721 tcacggtcat ttaccttttc ctgaactgga gcgcttttga tgctacgcga gtgtgattac
     3781 agtcaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     3841 cagggcagca atagcaaagc ctttttaggt cgcccagtca ccgggcaaac gctggatgtg
     3901 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgctcgtgcc
     3961 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc
     4021 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     4081 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     4141 accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4201 gatctctcac ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctca
     4261 atgaaagtgt taccgcgacc gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa
     4321 gaagccatga gtgaaatcgc cgagtggcaa ctccagccat tacccattag tggcttatgt
     4381 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcagcg
     4441 cgtgaactgc tgggtggcga agaggttgtg ggtcagttct ggcagcaatt gcgtgaacaa
     4501 caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg
     4561 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt acgctggctg
     4621 aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4681 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4741 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     4801 gcggaacttt gaggagcagg ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc
     4861 gcgctggaag ccgacagcat cctgtgcgcc tgtgttcact gcggattttg taccgcaacc
     4921 tgcccaacct atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg
     4981 attaaacagg tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc
     5041 tgcctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg
     5101 ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg
     5161 ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag
     5221 gtagggctgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa
     5281 acggtgaaag ctaaaccgcg tccgccgctg cgccataagc gtcgggtttt aatgctggaa
     5341 ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat
     5401 cgtctgggga tcagcgtcat gtcagctaac gaagcaggct gttgtggcgc ggtggactat
     5461 catcttaatg cgcaggagaa agggttggca cgggcgcgca ataatattga tgcctggtgg
     5521 cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc
     5581 gtcaaagagt atgggcagat gctgaaaaac gatgcgttat atgccgataa agcgcgtcag
     5641 gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg
     5701 gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa
     5761 aagctgaacg gcgaagtgga aaaagtgtta cttcgtcttg gatttacctt aacggacgtt
     5821 cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg
     5881 gcacgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc
     5941 gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt
     6001 cactggattg aaattgtaga acaagccctt gaaaaggaat aaaaaaatga aaactaaagt
     6061 cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa
     6121 aaataactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag
     6181 tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc
     6241 cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc
     6301 gttcgtgact gcgccgttac tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg
     6361 gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc
     6421 gaaagtggca gcagcggtgt tggcgaaata agcgaaaacg aggagagaaa caatgagtca
     6481 aaccataacc cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatggaga
     6541 agttttgccg ggcgtggaac tggacgctgc tgcattctgg cacaatgttg atgagatcgt
     6601 tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc
     6661 gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc
     6721 tttctttcgt gaactgggct acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac
     6781 gggcattgac agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcaatgaa
     6841 cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata
     6901 cggcagcgac atcatcccgc aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg
     6961 tgagcaggtt atcgcctggg ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg
     7021 cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa
     7081 aaatggtaaa gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcgatgc
     7141 cgctgcgccg acctgcattt tgctgaaaaa taacggcctg catattgaac tgcaaattga
     7201 tgccaacggg cggattggca aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc
     7261 tgctatcagt accattctcg actgcgaaga ttcggtcgcg gcggttgatg cggaagataa
     7321 aatcctgctg taccgcaatc tgctgggcct gatgcagggg actctgcaag agaaaatgga
     7381 gaaaaacggt cggcaaatcg tacgtaaact gaatgacgat cgtcagtaca ccgccgccga
     7441 tggctctgaa atttctctgc acggacgctc gttgctgttt atccgcaacg tgggtcattt
     7501 gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag gtattcttga
     7561 tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac
     7621 tggcagcgtc tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa
     7681 caaactgttt acccgcattg agacaatgct cggtatggca ccgaataccc tgaaaatggg
     7741 cattatggat gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg
     7801 caaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc
     7861 ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa
     7921 agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca
     7981 aatcggtaaa ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg
     8041 cgaccaactg cgtgccgggg caaacacagc ctgggttccg tcaccaaccg ctgctacgct
     8101 ccatgcgctg cactaccacc ataccaacgt acagagcgtg caagccaaca ttgcccagac
     8161 cgagttcagt gctgaatttg aaccgctgct ggacgatctg ctgactattc cggtggctga
     8221 aaacgctaac tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat
     8281 tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat
     8341 tcacaacgtg gcgctgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc
     8401 caactggtta cgtcacggta ttctgaccaa agaacaggtg caggcgtcgc tggagaatat
     8461 ggcgaaagtg gttgatcagc aaaatgctgg cgatccggct tatcgtccga tggcggggaa
     8521 tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca
     8581 gccaaacggt tataccgaac cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca
     8641 ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg
     8701 tagtacattt atcccggaaa taaccggagc gattatcagg gtttaacaat attaaatacc
     8761 cgtagcgcat tactagcggg cctaaaacaa caatatggac atcttaacga tactctgcgc
     8821 ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg
     8881 cgttagcggc gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt
     8941 ttgcttatta caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat
     9001 ggttacctgg acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc
     9061 cctgatcccg ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt
     9121 tgctggagca ataaccctta tattatctat cctgattgcg atattcgcct ttaaaatgcc
     9181 gattgatatg gcatttgctg ctgcgggcta tggctttatt tatggattat ggccaattgc
     9241 gtggattatt gtcgcggcgg tgttcctgta taaattaacc gttgccagcg ggcagttcga
     9301 tattatccgc agctcggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat
     9361 tggtttctcc tttggtgcgt tgctggaagg agctgctggc tttggtgcga cggtggcgtt
     9421 tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca
     9481 cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct
     9541 ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc
     9601 tgggggaagc ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc
     9661 ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttattcctta aagtctggcg
     9721 gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa
     9781 taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc
     9841 gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa
     9901 agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca
     9961 tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc
    10021 ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg cggcgattat
    10081 ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct
    10141 aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcattcg ccttcgtcac
    10201 caactattcc ggcatgtcca ccacgctggc gctggtactg gcaggtacag gcgtgatgtt
    10261 cccgtttttc tcaccgtttc tcggctggct gggcgtattc cttaccgggt cggacacttc
    10321 ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga
    10381 cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca
    10441 atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactgttccg
    10501 ctacaccgtg aagcacagtc tgatttttgc cagcgttatc accctgctgc aggcgtatgt
    10561 gtttaccggg atgttagtct cgtaaatcaa taaagccgga tgaaggtaaa tatcacccgg
    10621 ctttccattt gtctgtcgtt tatctcgcca gggaaccgtt tttacgattg gcttctttat
    10681 acgtagtctc cgttttacga tgcgaattgt ttaatattta tacaacttgg cttctgtgct
    10741 ggttataaag tacccacttt catgagatca attttagtat gttattacat tgcttcatct
    10801 gttgctttca tgacgaatgg cgttttttct cttatttata tttttcacta gaacaatgag
    10861 tagatcaaaa ttcccaaaac tcccaaaaac ataagattat tcgcaaagct gattttgcag
    10921 aataatgaga acttttaaga tttgtcgagg atcaatttta agcaatttat ctccacttgt
    10981 tacataaaag atgccgttag gttaataagt taatcttgct ataacagtgt tcgttactgc
    11041 ctattaataa agatatttgg gatagggtaa cgtggatgag gattcgaaat catccgtctt
    11101 gcggaaagtg aaatgttggc agatgttaat attatgtatg tattttaact ccaacaacac
    11161 accaatcatt ttgttagaaa taagaaaatt tatggttaaa taatttggtg attatgaagc
    11221 attaa
//
LOCUS       DAZPFP010000121        11120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPFP010000121 DAZPFP010000000
VERSION     DAZPFP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.121"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            15..314
                     /locus_tag="ACHUR4_002906"
     CDS             15..314
                     /locus_tag="ACHUR4_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1456409.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(434..613)
                     /locus_tag="ACHUR4_002907"
     CDS             complement(434..613)
                     /locus_tag="ACHUR4_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1456410.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            687..2048
                     /gene="pabB"
                     /locus_tag="ACHUR4_002908"
     CDS             687..2048
                     /gene="pabB"
                     /locus_tag="ACHUR4_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1456411.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2052..2630
                     /locus_tag="ACHUR4_002909"
     CDS             2052..2630
                     /locus_tag="ACHUR4_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1456412.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            2814..4178
                     /gene="sdaA"
                     /locus_tag="ACHUR4_002910"
     CDS             2814..4178
                     /gene="sdaA"
                     /locus_tag="ACHUR4_002910"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1456413.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4309..5907
                     /locus_tag="ACHUR4_002911"
     CDS             4309..5907
                     /locus_tag="ACHUR4_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1456414.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5911..7461)
                     /gene="yoaE"
                     /locus_tag="ACHUR4_002912"
     CDS             complement(5911..7461)
                     /gene="yoaE"
                     /locus_tag="ACHUR4_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1456415.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7449..7607)
                     /gene="yoaL"
                     /locus_tag="ACHUR4_002913"
     CDS             complement(7449..7607)
                     /gene="yoaL"
                     /locus_tag="ACHUR4_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1456416.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7924..8895
                     /gene="manX"
                     /locus_tag="ACHUR4_002914"
     CDS             7924..8895
                     /gene="manX"
                     /locus_tag="ACHUR4_002914"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1456417.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8958..9758
                     /gene="manY"
                     /locus_tag="ACHUR4_002915"
     CDS             8958..9758
                     /gene="manY"
                     /locus_tag="ACHUR4_002915"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1456418.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9771..10622
                     /gene="manZ"
                     /locus_tag="ACHUR4_002916"
     CDS             9771..10622
                     /gene="manZ"
                     /locus_tag="ACHUR4_002916"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1456419.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10677..>11120
                     /locus_tag="ACHUR4_002917"
     CDS             10677..>11120
                     /locus_tag="ACHUR4_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1456420.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2724 a         2817 c         2814 g         2765 t
ORIGIN      
        1 ttaaggataa gaatatgcct gctgtaatag ataaagccct ggatttcatt ggtgccatgg
       61 atgtatcagc gccaacacca agttcgatga atgaaagcac ggcgaaggga atctttaaat
      121 atttaaaaga actgggagta cccgcgagtg ccgctgatat tacggcgcga gctgaccagg
      181 aaggctggaa ccctgggttc acggaaaaaa tggttggatg ggcaaaaaaa atggagacag
      241 gtgaacgctc tgtgattaaa aatcctgaat acttttcaac atatatgcag gaagaactaa
      301 aagcactggt ctgagttaaa tttatatcag cataaatggg tcaggacgct tttaatcaca
      361 tataaaaagc gtcctttttc tcacaatcaa atgatcatcg gcttctataa cgaaatcgat
      421 acggattagt gtgttactcg tcttcatcct caaaacgcgc cacgattagc tcgccggagt
      481 ggttggcacg cagctcttct gccaccagcg cgattgcctg gccgctactc atcccttgtg
      541 ccatcagttc ctggatccgc tcgacagctt tttgctgctg ttcatggatg agtgaaggta
      601 aacctgcaaa cattgttaac tcctgctaaa ttgttggcgc taattatttc atgctacccg
      661 gcacatagcc agtagagtca ggactgatga agacgttatc tcccactgtg attactttac
      721 cctggcgtca ggacgccgct gaattttatt tctcccgctt aagccacctg ccgtgggcga
      781 tgcttttaca ctccggctat gccgatcatc cgtatagccg ctttgatatt gtggtcgccg
      841 atccgatttg cactttaacc actttaggta aagaaaccgt tgttagtgaa agcgaaaaac
      901 gcacaacgac cactgatgac ccgctacagg tgctccagca ggtgctggat cgcgcagaca
      961 ttcgcccaac gcataacgaa gatttgccat ttcagggcgg cgcactgggg ttgtttggct
     1021 acgatctggg ccgccgtttt gagtcactgc cagaaattgc ggaacaagat atcgttctgc
     1081 cggatatggc agtgggtatc tacgattggg cgctcattgt cgaccaccag cgtcatacag
     1141 tttctttgct gagtcataat gatgtcaatg cccgtcgggc ctggctggaa agccagcaat
     1201 tctcgccgca ggaagatttc acgctcactt ccgactggca atccaatatg acccacgagc
     1261 agtacggcga aaaatttcgc caggtacagg aatatctgca cagcggtgat tgctatcagg
     1321 tgaatctcgc ccagcgtttt catgcgacct attctggcga tgaatggcag gcattccttc
     1381 agcttaatca ggccaaccgc gcgccattta gcgctttttt acgtcttgaa cagggtgcaa
     1441 ttttaagcct ttcgccagag cggtttattc tttgtgataa tagtgaaatc cagactcgcc
     1501 cgattaaagg cacgctacca cgcctgcccg atcctcagga agatagcaaa caagcagaaa
     1561 aactggcgaa ctcagcgaaa gatcgtgccg aaaatctgat gattgtcgat ttaatgcgta
     1621 atgatatcgg tcgtgttgcc gtagccggtt cggtaaaagt accagagctc ttcgtggtgg
     1681 aacccttccc tgccgtgcat catctggtca gcactataac ggcgcgacta ccagaacagt
     1741 tacacgccag cgatctgctg cgcgcagctt ttcctggtgg ctcaataacc ggggctccga
     1801 aagtacgggc tatggaaatt atcgacgaac tggaaccgca gcgacgtaat gcctggtgcg
     1861 gcagcattgg ctatttgagc ttttgcggca acatggatac cagcattact atccgcacgc
     1921 tgactgccat taacggacaa atatactgct ctgcgggcgg tggaattgtc gccgatagcc
     1981 aggaagaagc ggaatatcag gaaacttttg ataaagttaa taagatatta cgccaactgg
     2041 agaagtaaga cgtggaatac cgtagcctga cgcttgatga ttttttatct cgctttcaac
     2101 ttttgcgccc gcaaattaac cgggaacccc taaatcatcg tcaggctgcc gtgttaatcc
     2161 ccatcgtccg tcgaccgcaa ccggggttgt tgctgactca gcgttcaatt catctgcgta
     2221 aacacgctgg acaagtggca ttccctggag gtgcagtcga tgacacggac gcatcagtta
     2281 tcgccgccgc gctgcgcgaa gctgaagaag aggtcgctat accgccttcc gccgttgaag
     2341 ttatcggcgt gctgccgccc gtcgatagcg taactggcta tcaagtaacc ccagtggtcg
     2401 gcattatccc gcccgatctg ccgtatcgcg ccagtgaaga tgaagtctcg gcggtgtttg
     2461 aaatgccgct cgcccaggca ttacatctgg gtcgttatca ccctttagat atctaccgcc
     2521 gtggcgattc acatcgggta tggctgtcct ggtacgaaca gtattttgta tggggaatga
     2581 ccgcaggcat aattcgtgag ctggcgctgc aaattggtgt gaccccctga ctatacttat
     2641 ctttacatct acaaaacact acttgagaca atcatcgcaa tattagttaa atcgcggttt
     2701 ttgattagtt taattcatgt gaatagttaa gccagtcgcc gcgttccctc ttacactatg
     2761 cgctgttatt agttcgttac tggaagtcca gtcaccttgt caggagtatt atcgtgatta
     2821 gtctattcga catgtttaag gtggggattg gtccctcatc ttcccatacc gtagggccta
     2881 tgaaggcggg taaacagttc gtcgatgatc tggtcgaaaa aggcttactg gatagcgtta
     2941 ctcgcgttgc cgtggacgtt tatggttcac tgtcgctgac gggtaaaggc caccacaccg
     3001 atatcgccat tattatgggt cttgcaggta acgaacctgc caccgtggat atcgacagta
     3061 ttcccggttt tattcgcgac gtaaaagagc gcgaacgtct gctgctggca cagggacggc
     3121 atgaagtgga tttcccgcgc gacaacggga tgcgttttca taacggcaac ctgccgctgc
     3181 atgaaaacgg tatgcaaatc cacgcctata acggcgatga agtcgtctac agcaaaactt
     3241 attattccat cggcggcggt tttatcgtcg atgaagaaca ctttggtcag gatgctgcca
     3301 acgaagtgag cgtgccgtat ccgttcaaat ctgccaccga actgctcgcg tactgtaatg
     3361 aaaccggcta ttcgctgtct ggtctcgcta tgcagaacga actggcgctg cacagcaaga
     3421 aagagatcga cgagtatttc gcgcatgtct ggcaaaccat gcaggcatgt atcgatcgcg
     3481 ggatgaacac cgaaggtgta ctgccaggcc cgctgcgcgt gccacgtcgt gcgtctgccc
     3541 tgcgccggat gttagtttcc agcgataaac tgtctaacga tccgatgaat gtcattgact
     3601 gggtaaacat gtttgcgctg gcagttaacg aagaaaacgc cgccggtggt cgtgtggtaa
     3661 ctgcgccaac caacggtgcc tgcggtatcg ttccggcagt gctggcttac tatgaccact
     3721 ttattgaatc ggtcagccca gacatctata cccgttactt tatggcagcg ggcgcgattg
     3781 gtgcattgta taaaatgaac gcctctattt ccggtgcgga agttggttgc cagggcgaag
     3841 tgggtgttgc ctgttcaatg gctgctgcgg gtcttgcaga actgctgggc ggtagcccgg
     3901 aacaggtttg cgtggcggcg gaaattggca tggaacacaa ccttggttta acctgcgacc
     3961 cggttgcagg gcaggttcag gtgccgtgca ttgagcgtaa tgccattgcc tctgtgaagg
     4021 cgattaacgc cgcacggatg gctctgcgcc gcaccagtgc accgcgcgtc tcgctggata
     4081 aggtcatcga aacgatgtac gaaaccggta aggacatgaa cgccaaatac cgcgaaacct
     4141 cacgcggtgg tctggcaatc aaagtccagt gtgactaata cttcttactc gcccatctgc
     4201 aacggatggg cgaatttata cccgctttct cgtctgctgt aatattcccc actacacttc
     4261 cactgttgcg tcaggcgttt gtcgccatac gcttacaggg tggcccgcat gcaaaaagca
     4321 caacggatca ttaaaaccta tcgccgtaat cgaatgattg tttgtacgat ttgcgcactc
     4381 gttacgctcg cttcgaccct gagcgtgcga tttatttcac agcgtaactt aaatcaacaa
     4441 cgggtagtac aattcgccaa tcacgctgta gaggaattag ataaagtact gcttccccta
     4501 caggcaggta gcaaagtctt gcttccgctg attggtctgc cctgctctgt cgcccatttg
     4561 ccattacgta aacaggcggc aaaactccaa actgtgcgat ccattggcct ggtgcaagac
     4621 ggcacacttt attgctccag catttttggt tatcgcaatg tgcccgtcgt ggacattctg
     4681 gctgaacttc ctgcaccgca accactttta cgcctgacga tcgaccgtgc cctgattaaa
     4741 ggcagtccgg ttttgattca atggacgcct gcagcgggca gtagcaatgc tggggtcata
     4801 gagatgatta acatcgactt actgacggca atgctccttg agccacaact gccgcaaatc
     4861 agtagcgcca gcctgacggt ggacaaacgg catttgctct atggtaatgg gctggtagat
     4921 tcccttccgc aacctgaaga caatgaaaac tatcaggttt cttcgcaaag ctttcctttt
     4981 accattaacg taaatggtcc gggggctacg gcgctggcat ggcactatct tccaacacaa
     5041 ttaccgctgg cggtgctgct aagtttactg gtgggctata tcgcctggct ggcgaccgct
     5101 taccggatga gcttttcccg cgaaattaat ctgggcctgg cgcaacatga gttcgaattg
     5161 ttctgtcagc ctttgcttaa tgcgcgcagc cagcaatgta ttggtgtaga gattttgctg
     5221 cgctggaata atccgcgtca gggctggatt tcaccggatg tgtttattcc tatcgcggaa
     5281 gaacatcatt taattgtgcc actgacccac tatgtgatgg cagaaaccat tcgtcagcac
     5341 catgttttcc cgatgagtag tcagtttcat gttggcatta acgtcgcacc cagccatttt
     5401 cgccgtggtg tgctgataaa agatctcaat cagtactggt ttagcgctca cccgattcag
     5461 caactgatcc tcgaaatcac cgaacgcgat gccttactgg atgttgatta tcggattgcc
     5521 cgcgagctgc atcgtaaaaa cgtcaaactg gcgattgatg acttcggcac cggcaacagc
     5581 tcgttttcct ggcttgaaac attacgtcct gacgtgctga aaattgataa gtcatttacc
     5641 gcagctatag gttctgacgc ggttaactcg acggtgaccg atatcatcat cgcgctgggg
     5701 caaagactga atattgaact ggtggcggag ggcgtggaaa cgcaagaaca ggcgaagtat
     5761 ttgcgccgtc atggcgtgca tattttgcaa gggtatttgt acgcacagcc gatgccgcta
     5821 cgtgattttc ccaaatggct ggcgggcagc caaccgccgc ccgcccggca taatggacat
     5881 atcacgccca ttatgtcgtt acgttaacgc ttactcatct tcatcgtgcg ccggttgctc
     5941 tttaacaatg cgaaccagat caacacgata atcattggct tcaaagatgg tgatatgcag
     6001 tggccctaca tcaatcacat cgcccacacg gggaatgtga ccatttgccg agatcacgag
     6061 gcccgcgacc gtcgcgatat catcgtcatc ggcaaggtgc tcaacatcaa gcgcctgctg
     6121 caaggcatgc aaatctgtac cgccttttac cagccagccg ttaccatcgg taatgatttc
     6181 cggcgtttcg tcagcgtccg ggaattcacc cgcaatggct tccagcacat ccagcggcgt
     6241 gaccaagcct tgtaccacac caaactcgtt ggtcacgata acaaagctcc cgcgagcgcg
     6301 acgcagcacg cccagcaggt tgatcggatc gagggtttcc gggacgataa tcgccggaga
     6361 cgccgaagca atcgccgcca catcaacgcc ctcttccagc gccaccagca gttctttagc
     6421 acgtacgata ccgatgattt catccagttc accgcgacat accgggaaca gactgtgcgg
     6481 tgaagagagc agttgctcgc ggatttcatc gaccccgaga ttagcgtcaa cccagcttat
     6541 ttcaccgcgc ggcgtcatga tcccgcgcag agaacgcgac gccagcgtca gtacgccgtt
     6601 aatcatgtac cgttcttctt cggcaaatgc accttccggg atcggcatcg ggttatcggc
     6661 atcgtgctga acattggcct gacgtttccc gcccatcaaa cgcaggatgg catcggcagt
     6721 acgcgctcgc agcggcaaag tcgactggtg acgaataaag ttgcgacgcg caatctggtt
     6781 aaacacttcg ataatgatcg agaagccaat cgcggcatac aggtaacctt tcggaatgtg
     6841 gaaaccgaaa ccttctgcca ccagactcag accaatcatt aacaggaagc tcagacagag
     6901 caccaccacc gtggggtgct ggttaacgaa tcgcgtcagc ggtttcgatg ccagcaacat
     6961 aaccgccatc gcaatcacta ccgccgccat catcaccggc agatggttaa ccatccctac
     7021 tgcagtaatt accgcatcca acgagaagac ggcgtcaagg atgacaatct gtgtgacgac
     7081 cacccagaaa ctggcgtagc ctttaccgtg gccggaatca tgatcgcggt tttccagccg
     7141 ttcatgcagt tcggttgttg ctttgaacag caagaatatc cccccgaaca acataatcag
     7201 atcgcgtccg gagaaggaga aatccatgac ggtaaatagc ggtttggtca gcgtgaccat
     7261 ccatgaaatc agcgacagca gccccagacg cataatcagc gccagtgata accccagcaa
     7321 acgcgcttta tcgcgttgtt ttggcggcag tttgtcagca agaatggcga tgaagaccag
     7381 gttatcgata cccagcacaa tttcgagaac aacaagcgtg agtagccccg cccaaattga
     7441 ggggtccatt aagaattcca tgacaagctc ctgcttaagg aatagctatt cgacgccaga
     7501 aataatgcag gcgtaacgac aaaatgcaaa cgaaaggtgc ggcatagagt gccagaaagg
     7561 caggcgttaa aaggcctgat gctgaaatga cgtcggtgac gatccatact gcgggctact
     7621 gccctatact ccatggttgt taaacgggag ttaaacatat cagagacgcc tctgatttgg
     7681 caaagattta ccttcctttg caaacgaatg tgacaaggat attttacctt tcgaaatttc
     7741 tgataatcga aagttaaatt acggatcttc atcacataaa ataatttttt cgatatctaa
     7801 aataaatcgc gaaacgcagg ggtttttggt tgtagccctt atctgaatcg attcgattgt
     7861 ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt aacgataata aaggaggtag
     7921 caagtgacca ttgctattgt tataggcaca catggttggg ctgcagagca gttgcttaaa
     7981 acggcagaaa tgctgttagg cgagcaggaa aacgtcggct ggatcgattt cgttccaggt
     8041 gaaaatgccg aaacgctgat tgaaaaatac aacgctcagt tggcaaaact cgacaccact
     8101 aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc tgccagccgc
     8161 attgtcgtcg acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg
     8221 gaaacgttaa tggcccgtga tgatgacccg agctttgatg aactggttgc gctggcagta
     8281 gaaacaggcc gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa agccgcgcca
     8341 gcacccgctg ccgcagcacc aaaagcggct ccaactccgg caaaaccaat ggggccaaac
     8401 gactacatgg ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg tcaggtcgcc
     8461 acccgctgga ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct
     8521 gcggataccg ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac
     8581 gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc
     8641 gtaatgctgt tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa
     8701 atcacctctg ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac
     8761 gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaaa aactgaatgc acgcggtatt
     8821 gagctggaag tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc
     8881 aaaatcgata agtaacgtat tgtgttgatt atcattcagt tttcacactt aagtcttacg
     8941 taaacaggag aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag
     9001 cctgtatcgc aggtatggga tcaatcctcg atgaatttca gtttcaccgt ccgctaatcg
     9061 cgtgtaccct ggtgggtatc gttcttgggg atatgaaaac cggtattatt atcggtggta
     9121 cgctggaaat gatcgcgctg ggctggatga acatcggtgc tgcagttgcg cctgacgccg
     9181 ctctggcttc tatcatttct accattctgg ttatcgcagg tcatcagagc attggtgcag
     9241 gtatcgcact ggcaatccct ctggccgctg cgggccaggt actgaccatc atcgttcgta
     9301 ctattaccgt tgctttccag cacgctgcgg ataaggctgc tgataacggc aacctgacag
     9361 cgatttcctg gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg gctattccgg
     9421 ccgtcatcgt tgcgctgtct gttggtacca gcgaagtaca gaacatgctg aatgcgattc
     9481 cggaagtggt gaccaatggt ctgaatatcg ccgggggcat gatcgtggtg gttggttatg
     9541 cgatggttat caacatgatg cgtgctggct acctgatgcc gttcttctac ctcggcttcg
     9601 taaccgcagc attcaccaac tttaacctgg ttgctctggg tgtgattggt actgttatgg
     9661 cagtgctcta catccaactt agcccgaaat acaaccgcgt agccggtgcg cctgctcagg
     9721 cagctggtaa caacgatctc gataacgaac tggactaaca ggtgagcgaa atggttgata
     9781 caactcaaac taccaccgag aaaaaactca ctcaaagtga tattcgtggc gtattcctgc
     9841 gttctaacct cttccagggt tcatggaact tcgaacgtat gcaggcactg ggtttctgct
     9901 tctctatggt accggcaatt cgtcgcctct accctgagaa caacgaagct cgtaaacaag
     9961 ctattcgccg tcacctggag ttctttaaca cccagccgtt cgtggctgca ccgattctcg
    10021 gcgtaaccct ggcgctggaa gaacagcgtg ctaatggcgc agagatcgat gacggtgcta
    10081 tcaacggtat caaagtcggt ttgatggggc cgctggctgg tgtaggcgac ccgatcttct
    10141 ggggaaccgt acgtccggta tttgcagcac tgggtgccgg tatcgcgatg agcggcagcc
    10201 tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt gcgtctggca acccgttact
    10261 acggcgtagc gtatggttac tccaaaggta tcgatatcgt taaagatatg ggtggtggct
    10321 tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct gtttgtcatg ggggcattgg
    10381 ttaacaagtg gacacatgtc aacatcccgc tggtggtttc gaccatcacc gggcaggatg
    10441 gacagacccg tgtcaccacc gtgcaaacta ttcttgacca gttaatgcca ggccttgtac
    10501 cactgctgct gacatttgct tgtatgtggc tgctgcgcaa aaaagttaac ccgctgtgga
    10561 tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg ctgggactgt
    10621 aagactgtcg tacactaccg gggccttttg gccccgtttt tttatctgga ggattaatga
    10681 caatcacgga cctggtactg attcttttca tcgccgcact cctggccttc gcgatctacg
    10741 atcagttcat catgccccgc cgtaacggcc ccaccctgct ggcaattcct ttgctccggc
    10801 gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt cattcttatc tataacaacg
    10861 tcacgaatca tggcgcgcta ataacaacat ggttattaag tgcactggct ctgatgggtt
    10921 tttatatatt ctggatccgc attccgaaga tcatctttaa acaaaaaggt tttttcttcg
    10981 ccaatgtctg gattgaatat agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt
    11041 tggtgatgca attagaacag cgtcggctgt taatccgcgt tcgaaatatc gacgatctgg
    11101 aaaaaattta taagcttctc
//
LOCUS       DAZPFP010000122        11118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPFP010000122 DAZPFP010000000
VERSION     DAZPFP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.122"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="ACHUR4_002918"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="ACHUR4_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1456421.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHUR4_002919"
     CDS             1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHUR4_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1456422.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHUR4_002920"
     CDS             1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHUR4_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1456423.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHUR4_002921"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHUR4_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1456424.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHUR4_002922"
                     /pseudo
     CDS             2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHUR4_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2797..3102
                     /locus_tag="ACHUR4_002923"
     CDS             2797..3102
                     /locus_tag="ACHUR4_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1456425.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="ACHUR4_002924"
     CDS             3105..3509
                     /locus_tag="ACHUR4_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1456426.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="ACHUR4_002925"
     CDS             3499..3798
                     /locus_tag="ACHUR4_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1456427.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="ACHUR4_002926"
     CDS             3984..4280
                     /locus_tag="ACHUR4_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1456428.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHUR4_002927"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHUR4_002927"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR1456429.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="ACHUR4_002928"
     CDS             5629..5997
                     /locus_tag="ACHUR4_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1456430.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHUR4_002929"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHUR4_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR1456431.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="ACHUR4_002930"
     CDS             7205..7906
                     /locus_tag="ACHUR4_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1456432.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHUR4_002931"
     CDS             7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHUR4_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1456433.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHUR4_002932"
     CDS             complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHUR4_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1456434.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="ACHUR4_002933"
     CDS             complement(9643..10974)
                     /locus_tag="ACHUR4_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1456435.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         2836 a         2922 c         2729 g         2631 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagact
//
LOCUS       DAZPFP010000123        11059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPFP010000123 DAZPFP010000000
VERSION     DAZPFP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.123"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(194..1858)
                     /gene="glnS"
                     /locus_tag="ACHUR4_002934"
     CDS             complement(194..1858)
                     /gene="glnS"
                     /locus_tag="ACHUR4_002934"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1456436.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2061..4007)
                     /gene="nagE"
                     /locus_tag="ACHUR4_002935"
     CDS             complement(2061..4007)
                     /gene="nagE"
                     /locus_tag="ACHUR4_002935"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1456437.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4340..5140
                     /gene="nagB"
                     /locus_tag="ACHUR4_002936"
     CDS             4340..5140
                     /gene="nagB"
                     /locus_tag="ACHUR4_002936"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1456438.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5200..6348
                     /gene="nagA"
                     /locus_tag="ACHUR4_002937"
     CDS             5200..6348
                     /gene="nagA"
                     /locus_tag="ACHUR4_002937"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1456439.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6357..7577
                     /gene="nagC"
                     /locus_tag="ACHUR4_002938"
                     /gene_synonym="nagR"
     CDS             6357..7577
                     /gene="nagC"
                     /locus_tag="ACHUR4_002938"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1456440.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7625..8377
                     /gene="nagD"
                     /locus_tag="ACHUR4_002939"
                     /pseudo
     CDS             7625..8377
                     /gene="nagD"
                     /locus_tag="ACHUR4_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8428..8526)
                     /locus_tag="ACHUR4_002940"
                     /pseudo
     CDS             complement(8428..8526)
                     /locus_tag="ACHUR4_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8774..10438
                     /gene="asnB"
                     /locus_tag="ACHUR4_002941"
     CDS             8774..10438
                     /gene="asnB"
                     /locus_tag="ACHUR4_002941"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1456441.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10817..10893
                     /locus_tag="ACHUR4_002942"
     tRNA            10817..10893
                     /locus_tag="ACHUR4_002942"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10851..10853,aa:Met,seq:cat)
     gene            10902..10986
                     /locus_tag="ACHUR4_002943"
     tRNA            10902..10986
                     /locus_tag="ACHUR4_002943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10936..10938,aa:Leu,seq:tag)
BASE COUNT         2751 a         2852 c         2797 g         2659 t
ORIGIN      
        1 ttgtttgcta tttcttgccg gatgcggcgt gaacgcctta tccggcctac aaaagcacgc
       61 aaattcaata tattgcagag atcatgtagg cctgataagc gaagcgcatc aggcaattta
      121 gcgtttgaaa tttattgtct atttattgcc ggatgcgacg taaatgcctc acccggcttg
      181 gtagaactta aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt
      241 taaataccgg tttttcggcc gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct
      301 caaactggaa tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt
      361 tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca cctgggttag
      421 gcacgctgaa cagacgatca tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca
      481 cccagtgaat aacacctttg actttacgac catctgccgg atctttgctt aaggtgtcgg
      541 cgtcataagt acagaagatg gtggtgatat taccttcggc atctttctcc acgcgttcag
      601 ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact
      661 gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca aatttcaccg ctaaacggca
      721 cctgacggct ccccatttcc ggtttgttcg gatggttcgg catggtgacc atttcgcctt
      781 cgccctgata gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg
      841 cattttcgtt gagatcttca cggatgcagg attccagcga cgccatctca atggtgttgt
      901 cctgcttggt cacgccgatg cgtttgcaga actcacgaat agaagccgca gtgtaaccac
      961 gacgacgcag accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt
     1021 cggtcaccag caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg
     1081 agaattcgta ctggcgcgga tgaacaggaa tggtgatgtt atccagtacc cagtcgtaca
     1141 gacgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagcgcat
     1201 cgctgatgca atgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt
     1261 ggtgttcagc aaatttaatg cggtacagca ccggatcgcg cattacgata aatggcgacg
     1321 ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gcacgcattt
     1381 tttcgaacag cgccaagttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg
     1441 gctgcgtcag ggtaccgcgg tattcgcgga tctgttccgg tgtcagttca tcaacgtacg
     1501 ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg
     1561 agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat
     1621 cgacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc
     1681 ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca
     1741 gatagccatt cggctccggc gggaaacggg tgtgtactgt gatgtgctta ccactggcca
     1801 gatcttcatc gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg
     1861 tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct
     1921 gacaactgtt agttttttaa aattgataat ctaacaagga ttatggggat gattgatgaa
     1981 aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt
     2041 cgcatcaggc ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca
     2101 acatggccct gagctttaat gagcaagcca ctgaaatcgt cgatattgct gcaaaccacc
     2161 gggctaatca tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt
     2221 tgccctgcgc ttacctgcgc accctcttcc accagacgtt taaagccttt accttccagc
     2281 gctacggtgt cgatacccat atggacgacg atctccgcgc ctttttcggt ttccaggcag
     2341 aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt
     2401 ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca
     2461 ggaacctgat ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat
     2521 accgcgtttg gtacagcctg cggttttgct acaggcgcgg cagttgccgg agttgcttca
     2581 gctgacgcag cggctaccgg accacgggca acgactttct tcatcgcatc gccgatggat
     2641 tctgctttcg cgccaacaat cacctgaata gtctgtttgt tcagtttcac tacaccagaa
     2701 gcacccagac gtttacacat cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc
     2761 agacgggtaa tacaggcatc aatcgctttc aggttgtcag taccgccaac cgcagcaata
     2821 tagttggttg ccagttgagt cagaccttct tcagtattgc tgttggcttc ttcagtaacg
     2881 atctcgtctt ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa
     2941 ctgaacacca cgaagtagat agcgaagaag ataacgccca tcaccagcag catccagacg
     3001 ttctggctgg cggcaggcag gttatacatc aacgcgtagt cgatagcacc cgcagagaaa
     3061 gagaagccgg catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt
     3121 gcgtgcagga ggtacagcag cggcgcaagg aacatgaaca ggaattccag cggctcagtc
     3181 acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga
     3241 cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg
     3301 atcgggaaga agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg
     3361 atgtcgccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg
     3421 atggtgttca gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag
     3481 ataccggaac ccagcgcgcc cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc
     3541 tgtaccggcg gccagacgta accaaaaatg gccgccagca ccagacagaa gaagccggtg
     3601 gcgatcggca caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg
     3661 gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc
     3721 atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca
     3781 cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg
     3841 aagattaatg cgaggttatc aaaaattgca ccgcccgcct gggcaataaa cgcaacgtta
     3901 agtaaatctg gctgaccgaa tcgcagcaac agcgccgcca ccggcagcac cgcgataggg
     3961 agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta
     4021 cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttttactgcc
     4081 gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc
     4141 atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc
     4201 ccttttactg gctaaaccag aaaacttatt ttatcattca aaaaatcaag tcggattgac
     4261 gcctgtctgc gcaaatccag gttacgctta aagatgtcta atccgccaac ggcttacatt
     4321 ttacttattg aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa
     4381 atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccaactg ccgatcgtcc
     4441 gtttgtactg ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga
     4501 aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata
     4561 tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt
     4621 cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaatg ccccggatat
     4681 cgatgccgag tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt
     4741 tatgggcggt gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc
     4801 ttctcgtact cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt
     4861 tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacactgct
     4921 ggatgccgaa gaagtgatga ttatggtgct gggtagccag aaagcactgg cgctgcaggc
     4981 cgccgttgaa ggttgcgtga accatatgtg gaccatcagc tgtctgcaac tgcatccgaa
     5041 agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata
     5101 tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggtt
     5161 ccttgctcag ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt aacccagggc
     5221 cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg
     5281 attaaaagcg tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac
     5341 ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag
     5401 tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat
     5461 gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg
     5521 aaacagggcg tgcgcgttat gcgtgagtac ctggcaaaac atccgaatca ggcgttaggt
     5581 ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt
     5641 gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc
     5701 aaagtgaccc tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc
     5761 gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcaaa agccggtttc
     5821 cgcgcgggga ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt
     5881 gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct
     5941 gatggcctgc atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa
     6001 ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt
     6061 gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc
     6121 ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca
     6181 ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattga cgttgaaaaa
     6241 cgtctcggca cgctcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt
     6301 aaaatcacca agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga
     6361 caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg
     6421 cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa
     6481 gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga
     6541 tcaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg
     6601 aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcatgacgcc accatcactc
     6661 tgtttgatct cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc
     6721 agcagacgct ggaacatgcc ctgctgaatg ccattgctca gtttattgat agctaccagc
     6781 gcaagctgcg cgagctaatc gccatttcgg tgatcctgcc agggcttgtt gacccggaca
     6841 gcggcaaaat tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac
     6901 tggaagaacg ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg
     6961 cggagcacta cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc
     7021 gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggtaacg
     7081 tcggtgaaat tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact
     7141 ttggctgcct ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt
     7201 taaagcaggg ctaccagagc cgtgtgccac tggacgactg caccatcaaa actatctgca
     7261 aagccgcgaa caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc
     7321 tgggtaaaac catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgctg
     7381 gtgaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc
     7441 aagcgctgaa ggcgtttcgt actaatctgc cggtggtacg ttctgagctg gatcaccgct
     7501 cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc
     7561 atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta
     7621 gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt
     7681 cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt
     7741 gttgctgacc aactatcctt cgcagactgg gcaagatctg gcgaaccgct ttgccactgc
     7801 cggtgtcgat gtgccggata gcgtgtttta tacctctgcg atggcgaccg ccgatttcct
     7861 gcgtcgctag gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact
     7921 gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac
     7981 acgttcctac aactgggaca tgattcataa agcagcctat ttcgtcgcta acggtgcacg
     8041 ctttatcgcc accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt
     8101 gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc
     8161 gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cagtgattgt
     8221 cggcgataac ctgcgtaccg atattctggc gggcttccag gccggtctgg agacgattct
     8281 ggtgctttct ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg
     8341 gatttacccg tcagtcgctg aaatcgacgt tatctgaata ccacccacgt ctgcggacgt
     8401 ggttggctct gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca
     8461 gctcctgcaa tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca
     8521 tccggcatga acaaatcgca ctttatcagc aatttggccc ccgtaaaata taaccattca
     8581 ataaaacccg ctaaaaatta tcgattcatc aataaataca tcctcacacc agaccctttt
     8641 tcaccaatcg acaatcacca ttacgttttt tatttttgca ctgccaaata gcttgcgcac
     8701 agtgctgctt tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt
     8761 aacggagaag gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga
     8821 gctgcgtaag aaagcactcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc
     8881 cggtatttat gccagcgata acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt
     8941 taacgcgggg gcgcaacctc tctacaacca acaaaaaaac cacgtactgg cggtaaacgg
     9001 tgaaatctac aaccaccagg cattgcgcgc tgaatatggc gatcgttatc agttccagac
     9061 agggtctgac tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg aatttctcga
     9121 cgacttgcag ggcatgtttg cctttgccct gtacgacagc gaaaaagatg cctacctgat
     9181 tggtcgcgac catctgggga tcatcccact gtatatgggg tatgacgaac acggtcagct
     9241 gtatgtggcc tcagaaatga aatcgctggt gccagtttgc cgcacgatta aagagttccc
     9301 ggcggggagc tatttgtgga gtcaggacgg cgaaatccgt tcttattatc atcgcgactg
     9361 gttcgacttt gatgcagtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact
     9421 ggaagattcc gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc tactttctgg
     9481 tggtctggat tcctcaatta tttccgctat caccaagaaa tacgcagccc gtcgcgtgga
     9541 agatcaggaa cgctctgaag cctggtggcc gcagttacac tcctttgctg taggtctgcc
     9601 gggttcaccg gatcttaaag cagcccagga agtggcaaac catctgggca cggtgcatca
     9661 cgaaattcac ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat
     9721 cgaaacttat gatgtgacca ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa
     9781 gatcaaggcg atgggcatta aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg
     9841 cggttatctt tacttccata aagcgcccaa cgttaaagaa ctgcatgaag agacggtgcg
     9901 taaactgctg gccttgcata tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg
     9961 gggcgtggaa gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat
    10021 taacccgcag gataaaatgt gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg
    10081 ttttgagtca tacctgcccg caagcgtggc ctggcggcag aaagagcagt tctccgatgg
    10141 cgtcggttac agttggatcg acaccctgaa agaagtggcg gcgcagcagg tttctgatca
    10201 gcaactggaa actgcccgct tccgcttccc gtacaacacg ccgacctcaa aagaagcgta
    10261 tctgtaccgc gagatctttg aagaactgtt cccacttccg agtgccgctg agtgcgtgcc
    10321 gggcggtcct tccgtcgcgt gttcttcagc taaagcgatc gaatgggatg aagcgttcaa
    10381 aaaaatggac gatccgtctg gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga
    10441 aatgcggtac tgccccggag aaatccgggg ctgttttagt tattgcgctg cttgttgatt
    10501 ctgatatgca gtaaggaaat gttccgggtt tgccacatca ctgtttgaca actcatcact
    10561 gtaggccagt atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc
    10621 tgtacgacaa aagaatggct gtagggtggg gaaaaacaca aagtaaacaa taattgacga
    10681 atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga
    10741 aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt
    10801 atcgcgaaaa aaagatggct acgtagctca gttggttaga gcacatcact cataatgatg
    10861 gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg
    10921 tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc
    10981 gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt
    11041 tttgataccg gcattccct
//
LOCUS       DAZPFP010000124        10939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPFP010000124 DAZPFP010000000
VERSION     DAZPFP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.124"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHUR4_002944"
     CDS             <1..794
                     /locus_tag="ACHUR4_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR1456442.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHUR4_002945"
     CDS             complement(744..1937)
                     /locus_tag="ACHUR4_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456443.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUR4_002946"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUR4_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1456444.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUR4_002947"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUR4_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1456445.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUR4_002948"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUR4_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1456446.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUR4_002949"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUR4_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1456447.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUR4_002950"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUR4_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1456448.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHUR4_002951"
     CDS             complement(10573..10716)
                     /locus_tag="ACHUR4_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456449.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2725 a         2758 c         2844 g         2612 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaat
//
LOCUS       DAZPFP010000125        10801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPFP010000125 DAZPFP010000000
VERSION     DAZPFP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.125"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /gene="chbB"
                     /locus_tag="ACHUR4_002952"
     CDS             complement(<1..264)
                     /gene="chbB"
                     /locus_tag="ACHUR4_002952"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1456450.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVD"
     gene            complement(563..901)
                     /gene="osmE"
                     /locus_tag="ACHUR4_002953"
     CDS             complement(563..901)
                     /gene="osmE"
                     /locus_tag="ACHUR4_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1456451.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1103..1930
                     /gene="nadE"
                     /locus_tag="ACHUR4_002954"
     CDS             1103..1930
                     /gene="nadE"
                     /locus_tag="ACHUR4_002954"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1456452.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            2160..3047
                     /gene="cho"
                     /locus_tag="ACHUR4_002955"
     CDS             2160..3047
                     /gene="cho"
                     /locus_tag="ACHUR4_002955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1456453.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3007..3582)
                     /gene="ves"
                     /locus_tag="ACHUR4_002956"
     CDS             complement(3007..3582)
                     /gene="ves"
                     /locus_tag="ACHUR4_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1456454.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3785..4270)
                     /gene="spy"
                     /locus_tag="ACHUR4_002957"
     CDS             complement(3785..4270)
                     /gene="spy"
                     /locus_tag="ACHUR4_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1456455.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4600..5568)
                     /gene="astE"
                     /locus_tag="ACHUR4_002958"
     CDS             complement(4600..5568)
                     /gene="astE"
                     /locus_tag="ACHUR4_002958"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1456456.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5572..6893)
                     /gene="astB"
                     /locus_tag="ACHUR4_002959"
                     /pseudo
     CDS             complement(5572..6893)
                     /gene="astB"
                     /locus_tag="ACHUR4_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(6890..8368)
                     /gene="astD"
                     /locus_tag="ACHUR4_002960"
     CDS             complement(6890..8368)
                     /gene="astD"
                     /locus_tag="ACHUR4_002960"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR1456457.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8365..9399)
                     /gene="astA"
                     /locus_tag="ACHUR4_002961"
     CDS             complement(8365..9399)
                     /gene="astA"
                     /locus_tag="ACHUR4_002961"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1456458.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9396..10616)
                     /gene="astC"
                     /locus_tag="ACHUR4_002962"
                     /pseudo
     CDS             complement(9396..10616)
                     /gene="astC"
                     /locus_tag="ACHUR4_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
BASE COUNT         2616 a         2959 c         2672 g         2554 t
ORIGIN      
        1 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
       61 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      121 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      181 ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg cagaacaaaa
      241 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      301 cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      361 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      421 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
      481 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
      541 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
      601 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
      661 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
      721 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
      781 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
      841 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
      901 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
      961 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     1021 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     1081 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     1141 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     1201 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     1261 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     1321 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     1381 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     1441 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     1501 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     1561 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     1621 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     1681 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     1741 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     1801 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     1861 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     1921 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     1981 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     2041 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     2101 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     2161 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     2221 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     2281 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     2341 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     2401 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     2461 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     2521 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     2581 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     2641 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     2701 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     2761 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     2821 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     2881 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     2941 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     3001 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     3061 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     3121 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     3181 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     3241 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     3301 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     3361 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     3421 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     3481 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     3541 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     3601 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     3661 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     3721 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     3781 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     3841 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     3901 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     3961 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     4021 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     4081 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     4141 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     4201 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     4261 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     4321 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     4381 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     4441 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     4501 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     4561 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     4621 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     4681 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     4741 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     4801 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     4861 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     4921 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     4981 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     5041 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     5101 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     5161 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     5221 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     5281 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     5341 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     5401 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     5461 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     5521 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg cccctccctc
     5581 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     5641 ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac gatcgcggta
     5701 gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca tcaccgccgg
     5761 attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc
     5821 gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa tcgggttgtc
     5881 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     5941 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     6001 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     6061 cacccgcgtg ccagtaactg tgactggcga gcgaacgcct gttggtggca aaacagcacc
     6121 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc tctggtcgat aacgtccggg
     6181 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc caccgcctcg
     6241 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccttcttct
     6301 cgcccgtaga caaaaagtag catacccggt tcaccgtaat gaccgccgag acgattgtgg
     6361 tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg gacgctaaat
     6421 ttctcttcgt ccttaaaaat cgcttttaac agcgattcag tgacgggcgc ttccagcgaa
     6481 cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc cagcgtatcg
     6541 gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga actgacgctg
     6601 gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc gctaccactg
     6661 aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcggaat cacggcctgg
     6721 gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc cgccagtcgc
     6781 gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga caggcccgcg
     6841 taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt catcgcacca
     6901 cctcatcgga aaaatccagc ccggggttaa gcgtggcggg caatgttaac gagtccgact
     6961 ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
     7021 taccggaagc accaatgccg ccgaatgggg cggtactggc agcaccagta agcggtttgt
     7081 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg
     7141 gggaaaccag accgcaaaag agtccgaagc gagtgttgtt cgccatccga atcgcctcat
     7201 caaaagtatc ataacgccag acgcgcaaaa acggcccgaa cacctcttca tccggcacac
     7261 cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa cgatgtccct gcttgtaata
     7321 agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag gcagtaacca
     7381 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
     7441 agttgcccgg cgttaatcgc tggctgacgg caaccagact agcaagaaac gcatcgccct
     7501 gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
     7561 acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca tcgataatta
     7621 gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg gagagctggc
     7681 gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg agatcctcca
     7741 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc acgcccggcg
     7801 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt gtcagttcgc
     7861 tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt ccgttcggca
     7921 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
     7981 ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg tgatacgcct
     8041 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
     8101 gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt tccagcaatc
     8161 cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc cagcgcggaa
     8221 acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca tcattgcctt
     8281 gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc tggcccgtta
     8341 tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
     8401 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
     8461 acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat aattttcatt
     8521 ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
     8581 ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc
     8641 gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg cgcgggcagg
     8701 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata aaaagtgggt
     8761 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
     8821 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg
     8881 tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt cattaaactt
     8941 gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc cgttgccctc
     9001 tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
     9061 actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg cgtgaaccaa
     9121 cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct caatagcgca
     9181 aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcgct
     9241 tttgggcagt tcaccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
     9301 ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc
     9361 cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg gctaacaaag
     9421 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctgacattg
     9481 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
     9541 gccgcttcca gagagatctg tttcgcttgc ccggcgtaat cggcattcag tacacagcca
     9601 atcaacaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt aatagtatta
     9661 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggtgtgttg
     9721 atgagctcca gcactttgcc tgctaccgcc gaggccagcg gattaccgcc ataggtggtg
     9781 ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc
     9841 gggaaaccgc cgcccagcgc tttggcggtg gttaacagat cgggcgtcac gccgtagtgc
     9901 atataggcat acagctcacc agtgcgcccg acgccggttt gtacctcatc aaaaatcagc
     9961 aaggcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt gctggctggc
    10021 accacaccgc cttcgcccta gatgggctcg acaatcaccg cacaggtggc gtcgtcaatc
    10081 agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc cggcagtggc
    10141 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    10201 tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc tgccgtagcg gtcgtgagcg
    10261 aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt acaaaagaag
    10321 acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg
    10381 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt
    10441 tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    10501 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa gggtgccgga
    10561 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    10621 acctctacag taaataattc gttatttgta tgttaacaat aagtaatgtt tgcgctgtaa
    10681 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc ggaggctaac
    10741 tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa agtgaatacg
    10801 t
//
LOCUS       DAZPFP010000126        10799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPFP010000126 DAZPFP010000000
VERSION     DAZPFP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.126"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            151..864
                     /locus_tag="ACHUR4_002963"
     CDS             151..864
                     /locus_tag="ACHUR4_002963"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1456459.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            896..1654
                     /locus_tag="ACHUR4_002964"
     CDS             896..1654
                     /locus_tag="ACHUR4_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456460.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            1700..3049
                     /locus_tag="ACHUR4_002965"
     CDS             1700..3049
                     /locus_tag="ACHUR4_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1456461.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3049..3873
                     /locus_tag="ACHUR4_002966"
     CDS             3049..3873
                     /locus_tag="ACHUR4_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456462.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            3885..4445
                     /locus_tag="ACHUR4_002967"
     CDS             3885..4445
                     /locus_tag="ACHUR4_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1456463.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4476..5546
                     /gene="lptF"
                     /locus_tag="ACHUR4_002968"
     CDS             4476..5546
                     /gene="lptF"
                     /locus_tag="ACHUR4_002968"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1456464.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            5543..6622
                     /gene="lptG"
                     /locus_tag="ACHUR4_002969"
     CDS             5543..6622
                     /gene="lptG"
                     /locus_tag="ACHUR4_002969"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1456465.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6658..7829)
                     /locus_tag="ACHUR4_002970"
                     /pseudo
     CDS             complement(6658..7829)
                     /locus_tag="ACHUR4_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(7829..8077)
                     /locus_tag="ACHUR4_002971"
     CDS             complement(7829..8077)
                     /locus_tag="ACHUR4_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1456466.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8110..9024)
                     /locus_tag="ACHUR4_002972"
     CDS             complement(8110..9024)
                     /locus_tag="ACHUR4_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1456467.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9021..10709)
                     /locus_tag="ACHUR4_002973"
     CDS             complement(9021..10709)
                     /locus_tag="ACHUR4_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR1456468.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2604 a         2669 c         2822 g         2704 t
ORIGIN      
        1 tccattaaaa atatttattt ggttaatatg tttttatgaa agcgtaattc aggtcaatgt
       61 cacaattagt cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
      121 ttcaatacat tatttactgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
      181 tccacgccag tgcgtgttat tgccattgta ggatgtgatg gttcaggtaa atcgaccctc
      241 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacacat ttatctcggg
      301 caatcgtccg ggcgaattgg cgaatggatt tcacagctcc ctgttattgg cgcaccttgt
      361 gggtgttatc tgcgaagtaa agcggcacat gtacacgaaa agcccttaac tccgcctggc
      421 aatattactg cgctggttat ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa
      481 atgttgtgta aaagccagca aggctatctg ctcatcaccg accgctaccc gcaggttgaa
      541 gtgccgggtt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
      601 ataaaaatgt taaggcagcg cgagttgaag ctgtaccaat ggatggcgtc ttatttaccc
      661 gtattgttga ttcgtcttgg cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac
      721 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacatt caatggcgcc
      781 aagatccttg aactggatgg tcggcaacca gccgatgaaa ttatgcaagc gtcactacgc
      841 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
      901 tgcactacaa acacaaactg ttaatagtac aaccgcgccg cagcccaact acattccggg
      961 gctgattgcg gtggtcgggt gtgatggcac cggtaaatcc acgctgacca ccgacctggt
     1021 gaaatcgctg caacaacact ggcaaaccga gcgccgctat ctggggctgc tctccggcga
     1081 agacggcgac aaaatcaaac gattgccgtt ggttggcgtc tggctggaac ggcgattggc
     1141 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccggcgctat gggcggcggt
     1201 gattatgtac tgcttctcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     1261 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     1321 ttatgacgga ccggggatcg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     1381 gcagcgcgaa cggagtttat accaaaaaat ggcgcaatac cgtcctgaat taatcattcg
     1441 tttggggatt gatattgata ctgccatctc ccgcaagcct gaccatgagt atgccgagct
     1501 gcaggacaaa atcggcgtca tgtcgacgat tggctataac ggcacaaaaa ttcttgagat
     1561 tgattccaga gcgccctaca gcgaagtgct ggaacaggta cagaaggcgg tttctctggt
     1621 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatattt tttactatca
     1681 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     1741 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg agagcgcctt
     1801 gctggggctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggtgt
     1861 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     1921 gcagctggtg gttcagtacg gaacgccagc cttgaccaac aataatccgc agcaatttcg
     1981 caatgtcgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     2041 cggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     2101 gctggcggcg ctgtattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     2161 tctgcgtgcg gtagatcgct ttgatttgat tgctgtacag caggcgacga aaccttttct
     2221 gcgcgcagcg gggagcgtcg cagcctggta ttttgacttt ggttttgtgg gttttgttat
     2281 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg ccgcgcgcga
     2341 attacgccgt agaaatatcc ataacgcctt caaattgaat ctgtttgagt ctgcccgaca
     2401 cattaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccactcca tctggtcggc
     2461 gcgtaactcg tgcagcacag tgttggtggg gatcgtgtta ggacccgctg ccgccgggtt
     2521 atttaaaatc gccatgacat tcttcgacgc cgccggaacg ccagcgggtt tgctgggtaa
     2581 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     2641 tgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtggcgctcg cggtgttgat
     2701 cgtcggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     2761 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggcttcccgc aggagtcatt
     2821 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     2881 ctacatcgta ttgctgttca tgttctgtca tctgtttggt gtgctgggag cagcgtttgc
     2941 ttacttcggt gggcaatgtc tggacgtggc gctctcgtta attccaacct taaaggcatt
     3001 ttttcagcgc cattccttgc tttataacgc agccggagag aaatcctgat gatgagaaaa
     3061 tatttcccgc ttgaggcgag cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt
     3121 gatgcacagg ttagtttgcc gccgactatt gcactaagtt gtaccaccga aatcattcat
     3181 gataattacg cgttatgcct gcaattctgg ctcaatggcg ttgatcgtca ggaattactg
     3241 cgtttagttc gcaaacaagc caaaggcgat gagctgactg ctgatgagcg caaacaattt
     3301 aaatacatgc gagcgcgcta caagcacctg cgttttgcgc aacggctgta tttaaagaag
     3361 catcaggccg gatttttatt cggcaaaacg accgtttttc tggggcgttt tcaggacggc
     3421 tttcgtaacg gtaagaaaaa tattgtgtcg tattacggca acctgttacg gatttattta
     3481 agctctccgg tctggtcgtt ggtgaactat tcattacgcc atagccagtt agaaagcgta
     3541 agcagtttta ttgcctatcg gcaaaagcaa atgcataccc tgaaagagat tattgccaaa
     3601 ccgcgactaa ccggcaggga atttcatgat gtgcgaaaaa tcatcagcca gcaggtttct
     3661 tattacgaca ccttaagatc gcttgatcct gaaaataaag aggcgttaca gatttcccgt
     3721 ttcctcgccg ccattaatgg cctgatggga gataaacacg acgacatggt ggcggatgat
     3781 atggaaaacc gccagtcgta ttatgcaccg ttggcgctgg atagcgatat ttgtcagcgc
     3841 ttagagctgc tgatttcgcg ttttccgcta tagccggagc aatgatgtca tctcgtttga
     3901 ttattgcgtt gattatcatg ctcttagccc cgggtgtgca ggcacacaat ttcgtcactg
     3961 gcaaaacggt tacccctgtt tatatccagg aaggcggcga gctgcttctg aatagcgacg
     4021 acgaaattca ctaccaaaag tggaacagca cacaattagc gggaaaagtc cgcattattc
     4081 agtatatcgc cgggcgtaag tcggcgaaga aaaagaactc actgttaatt aaagcggttg
     4141 aagcggcaaa ctttccgcag gatcgttttc agcccaccac cattgtgaac accgatgatg
     4201 ccatctttgg tactggctat tttgttgttg gcaaaatcga aaaaaataaa cgccgctatc
     4261 cgtgggcgca attcgttatt gacggcaatg gacagggacg cgttgcctgg cgcttgccgg
     4321 agcagagttc aaccattctg gtgctgaata aagccgggca aattcagtgg gcaaaggacg
     4381 gcagcctgac gccggaagag gtagatcatg tcattgcgtt ggcgcagaaa ctgatcaatg
     4441 aataaaaaac tcaaaggatt gagggcgtta gtgagatgaa gctggttgaa cactacatca
     4501 tgcgtggtac gcgccgtctg gtgctgatta tcgtcggttt tctgatcttt attttcgcca
     4561 gctactccgc acagcgttat ctgaccgaag cggcaaatgg gacattagct cttgatgttg
     4621 tgctggatat cgttttctac aaagtactga ttgcactgga gatgttgtta cctgttggtc
     4681 tgtatgtgtc agttggcgtg acgctagggc agatgtatac cgactcggaa attaccgcta
     4741 tctctgcggc ggggggcagt ccgggacgct tgtacaaagc cgttctttat ctggcgatac
     4801 cgctaagtat ttttgtcacc cttctgtcga tgtatggtcg tccgtgggct tatgcgcaga
     4861 tttatcaact ggagcagcag tcgcagtcgg agctggatgt tcgccagttg cgggcaaaga
     4921 aatttaacac taacgataac ggacgaatga tcctttcgca gacggttgat caggataata
     4981 atcgcctgac tgacgcgctg atttatactt ctactgccaa tcgaacccgt attttccgcg
     5041 ccggttcggt tgatgtggtt gacccatcac ccgagaaacc gaccgttatg ttgcacaacg
     5101 gcactgccta tcttctcgat catcaggggc gtgacgacaa cgaacagatc taccgtaatc
     5161 tgcaattaca tctgaatccg ctggatcaaa gcccgaacgt caaacgcaaa gcgaaatcgg
     5221 tcacggagct ggcgcgctcc gcctttcctg ccgatcatgc cgaactgcaa tggcgacaaa
     5281 gccgtggcct gaccgcattg ttgatggcgc tgctggccat ttcattaagt cgggtaaaac
     5341 cgcggcaagg gcgattttca acgttattgc cactgacgtt gctgtttatt gccatttttt
     5401 atggcggcga cgtctgccgt acactggtgg ctaacggcgc gattcccctc attcctggtt
     5461 tgtggttagt gcccggactc atgctaatgg gcctgctgat actggtcgca cgtgacttct
     5521 ctttgctgca gaaattttcc cgatgaatgt tttcagtcgc tatttaatcc gtcatctctt
     5581 tctcggtttt gccgccgctg cagggctatt gctgccgctt tttaccacct tcaacctgat
     5641 taacgaactg gatggtgtca gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt
     5701 gctaatgacc ttaccgcgca cactggtcga actttcgccg tttatcgcgt tattgggtgg
     5761 tattgttgga ctggggcagt tatcgaaaaa cagtgagctt accgccattc gcagcacggg
     5821 gttttctatc ttccgtattg cactggtggc gctggttgcc gggatattgt ggactgtttc
     5881 gttaggcgcg atagatgagt gggtggcgtc gccattgcag caacaggcgt tgcaaatcaa
     5941 atcgaccgcc accgcgttgg gggaggacga tgacattacc ggcaatatgc tgtgggccag
     6001 gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag cagggccagc cggtgggcgt
     6061 ggagatattt cattatcgcg acgatctctc gcttgaatcc tacatttatg cacgcagtgc
     6121 caccattgaa gacgacaaaa cgtgggtcct gcatggtgtg aatcataaaa aatggctgaa
     6181 tggcaaagaa acgctggaaa cattagataa tcttgcctgg caatcggcct tcaccagtat
     6241 gaatcttgaa gagttatcaa tgccggggaa tactttttct gtccgtcagc ttaatcatta
     6301 catccattat ttgcaggaaa ccgggcaacc cagcagcgaa taccaccttg cactgtggga
     6361 aaaactgggg caacccatcc tgaccctggc gatgattttg ctggctgtgc cgtttacctt
     6421 tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct gtaggcgtca tcgtgggctt
     6481 actcacctgg atcagctatc aaatcatggt taatctggga ttgctatttg cgttaagcgc
     6541 acctgttacc gcgctcggtt taccggtagc gtttgtgttg gtggcgttga gcctggtgta
     6601 ttggtatgac agacaacatt aaacccgccg tggcgcaaac accacggcgg gctcacatta
     6661 ctgcttcaga gtagcaaatg cctgaatgat ggcatcgatt tgctcacgac tgtgggcagc
     6721 atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg gcaggcggca ggataagatt
     6781 cacatacacg cccggcgaga taagctcgcg ccagatgcgt aaaccttcct ctttcgaacc
     6841 gattattact ggcactaccg ggctaatatg tgaacttaac tcataaccca gtttttgcag
     6901 accgtcatac agatggttgg cgttatccat cagcttttgc cgcagctctg gttgtgtggc
     6961 aatagttctt aacgacgagc gcacggtcgc aatgcaggac ggagaaggag aggcggtaaa
     7021 aatatacggg cggctgccgt agcgcagcac ttccatcgct tccgaaacga cagcaaaacc
     7081 gccaatcgag gccagacttt tactgaaagt accaacgata atatccacat catcttcaac
     7141 gccgacagct tcagccagtc cgcgccccgt tgcgcctaac acgccaaacg aatgcgcttc
     7201 atcgacaatc aaataaccac cgagacgacg tttgatatca acaatttccg ccagcggcgc
     7261 aacgtcaccg agcatgctgt agatgccctc aacgatgata atcgcctctt tcgcccgttc
     7321 accgaggcga accatgcgcc gttccagatc tttagcatcg ttatggcgga aacgaataat
     7381 ttccgcgcca ccgagagaac agacgtcata aatactggcg tggctgtcgg catccagtaa
     7441 caccacggcg ttatggtcgg caagtgcgct gatgacgcct aaattagcgg tataaccggt
     7501 tgaaaaacga tagcggtagg gcgattgaaa aactcggcaa tctctttctc taacgccagg
     7561 tgcggcgcat aactgccgtt tgccatacga gaaccggtgg tgccggtgcc ttgggccgct
     7621 aacgcggcct gaccttcgct gatggcatca tgattaaagg ttaaaccgag ataattattg
     7681 gtcccggcca gaatcacttt tttattgccg atccggcctt cggtggcgga atacacttca
     7741 tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa attgcagacg ctcgccagca
     7801 aggcgcgcat atttatcgta tagccccatt atttattctc cagccatgga agtagagttt
     7861 ccattaattg cgcaggggtt ttgacatcca gcagaatatt aataggaatg gaaatatcga
     7921 atctgtcttc cagcatcatt aagagatcca tcactttgat cgactccagg ccaagatcgt
     7981 tgacgagatc gctgtcgggc ttaatttcca caccgttttc gaccaaatcc tgtaaacagg
     8041 aaaggatgta atccattact atttcacgat ttaccataga aacgttaaca cacctttaaa
     8101 acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc aggctaatcc cgggaaacca
     8161 attgatatct tcagaaatac ggttattact tgccgaccag tcggcgtggg ttaattcacg
     8221 aattttcgag cgggtcagca taggttcttt acctgccagg cgactcaacg cggtactgat
     8281 atccgcaagg cctgtgagta ccggcagagg aatgtcaacc attcgcacgg aaccacaacg
     8341 ggctttggcg gcaagctgtc gtacgcgttg ccagtcatag ccaccggcga cgccatcgca
     8401 taattcatag gtttgcgtct gtacagtttc ggcgcttaac cactgaccca ctgcttgcgc
     8461 aaaatcggtg acgtgaagaa aagagagttg tgtatctggc gcaccaagtc gtggcagcag
     8521 gccgcgtagc atccagtcaa acagcggttt taactcttta tcgccgggac catacacggc
     8581 tgtcgggcga aaaacgccca gcgtaatttc gtccgccatt gctgccagtc gttgctcggc
     8641 gacgtatttg gattttgcat accaggagag ctcgggatgg cgcgccgcca gcgaagagat
     8701 aaacagaaaa cgttggcaaa agccgctctc ttttgctgct tgcatcaggc gcaggctgcc
     8761 gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg cgcacttgcc cggcgcaatg
     8821 gaccactacg ctggctccgg caaccagctc gctaagtgaa tgagtatctt ccagcgaacc
     8881 gcgcacccag gtgagattat cattgacgtg agcgcgggca gtacgcgtca atgcgcgaac
     8941 gtgaaagccg cgggcgagca ggttatcgat aatatattta ccgataaacc cggtagcgcc
     9001 cgtcaccgcg acagtttgat ttatgccagg gattcctgca cattaagact ggcagcataa
     9061 gccttctgat aacgtttttt cgcttccgca cgggcaggct tgccggagga cgttcgggga
     9121 atactgtggg gcggcaacag atcgatagcc gcggtcacgc caaattcgct ttggatccgt
     9181 gccgccagcg cgtggataag ctgcccgcga cgttcttcgt cgctgatccg acactggatc
     9241 tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg catcgccaga atgaatttcc
     9301 ggttcctgtt ccgctatata ttcaatatcc tgcggccaga tattacggcc acgaataata
     9361 atcagatctt taatgcgtcc ggtgacatac agataaccgt ccagcagata accgaggtcg
     9421 ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg aaacctggtc gccaaagtaa
     9481 ccgctcatca gactgggacc ggagatgcaa atatggccta ccacacgttc cgcgaccggc
     9541 atacctgctt cattgcggat ttcaatacca tgttccggca acgctttgcc gcagttgacg
     9601 aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg ctttaccctg atattcgagg
     9661 atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt catcagagaa gctgacagcc
     9721 agcgcatttt ctgccagtcc gtagcacggc atgaaagttt tattgtcaaa gttaacctga
     9781 cggaaacatt cagcgaattg atggagttgt tctgcggaga tcggctcagc accaatacca
     9841 gcgacgcgcc agcaggaaag atccagttca gcgagatctt tttcattcac gcggcgttgg
     9901 cacaattcat agccaaacgg cggcgcaacg gaaacggtgc cgcgattttt actgatcaat
     9961 ttaagccatt gcaaaggacg catggcaaaa tcctgagtgc gcaaataatc tactgaaagt
    10021 tgcgtggcga cggggggcag gagaaagccg accagtccca tatcatggta gaaaggctgc
    10081 caggagacgc agcggtcgct agggcgtaat ttaatgccat cgtggcttat agcacgtaga
    10141 ttagccatca cttcgcgatg ggtgataatg acgccacggg gaaaacgggt gctgccggag
    10201 gtgtactgga ggtaggcgat atcgttcgga actggacgct ggagcgcaac atcggcttcc
    10261 ggtaatgcct taaaccaagc gtggcttaaa acatgtaatt cggggttgtt atgcgtcgcg
    10321 gcattgacca gtggcaacca ctcatcacca gtgataatgg ctgcgggctg gcaacttgcc
    10381 agtaaaccct ggagtttggc gctccaggaa tcccgctgac cgacgcccat tggaatcgcc
    10441 aacgggacgg cgactaagcc agcatactga caggcaaaaa aagcctctac aaacccgcta
    10501 ctcgtttcgg caatcagcgc cacgcgttcg ccttttttca ggttcagcga taacaaccgc
    10561 ttcgcaccag cttcggcacg cgtttttaat gtctgatatt ccagttgatc ttcaagttgg
    10621 caacgtctgt cataaaaatt cattccggct ctactcagag cggcgtagtc caaagcatca
    10681 accagcgttg gaaaatcggc atagcgcata ggtagggaat gcgtaaagat tttattagac
    10741 atatacacca cgaaaaaatt ggatatcaaa aataaaatga caataatgcg gtggattat
//
LOCUS       DAZPFP010000127        10669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPFP010000127 DAZPFP010000000
VERSION     DAZPFP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.127"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1807)
                     /gene="selB"
                     /locus_tag="ACHUR4_002974"
     CDS             complement(<1..1807)
                     /gene="selB"
                     /locus_tag="ACHUR4_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1456469.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
     gene            complement(1804..3195)
                     /gene="selA"
                     /locus_tag="ACHUR4_002975"
     CDS             complement(1804..3195)
                     /gene="selA"
                     /locus_tag="ACHUR4_002975"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1456470.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3293..3901)
                     /locus_tag="ACHUR4_002976"
     CDS             complement(3293..3901)
                     /locus_tag="ACHUR4_002976"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1456471.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3972..5057)
                     /locus_tag="ACHUR4_002977"
     CDS             complement(3972..5057)
                     /locus_tag="ACHUR4_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1456472.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5060..5422)
                     /locus_tag="ACHUR4_002978"
     CDS             complement(5060..5422)
                     /locus_tag="ACHUR4_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1456473.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5959..7872
                     /gene="mtlA"
                     /locus_tag="ACHUR4_002979"
     CDS             5959..7872
                     /gene="mtlA"
                     /locus_tag="ACHUR4_002979"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1456474.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7967..9115
                     /gene="mtlD"
                     /locus_tag="ACHUR4_002980"
     CDS             7967..9115
                     /gene="mtlD"
                     /locus_tag="ACHUR4_002980"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1456475.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9115..9702
                     /gene="mtlR"
                     /locus_tag="ACHUR4_002981"
     CDS             9115..9702
                     /gene="mtlR"
                     /locus_tag="ACHUR4_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1456476.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9714..9923)
                     /gene="yibT"
                     /locus_tag="ACHUR4_002982"
     CDS             complement(9714..9923)
                     /gene="yibT"
                     /locus_tag="ACHUR4_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1456477.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10207..10569
                     /locus_tag="ACHUR4_002983"
     CDS             10207..10569
                     /locus_tag="ACHUR4_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1456478.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2564 a         2883 c         2751 g         2471 t
ORIGIN      
        1 gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga atctgaattg
       61 ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact
      121 cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta cggtaataac
      181 gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc gtcaggcgca
      241 ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac ggctcgtcac
      301 caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag cccgctttgt
      361 gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt
      421 caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt cgcagacgtt
      481 cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa
      541 ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca gcctgaatat
      601 agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc gcccaagcga
      661 aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg ctatcattct
      721 gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta ccgcgacgcg
      781 gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca
      841 ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt tcagcaaggt
      901 tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg
      961 gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt gtgaacggct
     1021 ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt tccgcatcac
     1081 ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc tggttttgcg
     1141 catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt gaatcgccta
     1201 ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct tttacggtaa
     1261 atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt tccggcaact
     1321 gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg gtgataaaca
     1381 gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt tcaacctcat
     1441 caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc atcgggttac
     1501 cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg tcatcgcacg
     1561 ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg gaaagaaact
     1621 tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc ggccagtagg
     1681 catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg tcagcattta
     1741 cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa
     1801 taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag
     1861 ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac gccagcgggc
     1921 ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta atgccgcgct
     1981 cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg gcataacctg
     2041 taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt gtgcctggat
     2101 ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt cactcagagc
     2161 ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc
     2221 gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt ttttaccaac
     2281 gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca ccagactgac
     2341 gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact ggctaagatc
     2401 gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt tgccgagcgc
     2461 caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt tactggtatg
     2521 tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat tcgcgtgcgt
     2581 gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa catcgggaat
     2641 acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct ctttcccgct
     2701 ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga tacaggcatc
     2761 ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc
     2821 ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg caacggcttc
     2881 caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg ttcccgtcag
     2941 gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat cgacttcttg
     3001 cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc
     3061 gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat cacgcaaaga
     3121 aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat agagggaacg
     3181 cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga gaagggcgtt
     3241 taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg catcaagcct
     3301 ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc agtttgacta
     3361 agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga tatccgacag
     3421 cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg ccatcgacca
     3481 gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt aacaattcat
     3541 cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc ccggcatcca
     3601 taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg
     3661 gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc ggcgaatcaa
     3721 accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg ttaaattgcg
     3781 ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt atgccctttt
     3841 ctaacaacag aataaaaagt ttgcgtacaa acgggctggt gtagctaccg acgagtttca
     3901 tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc aaaacacagg
     3961 tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat tcttagcagc
     4021 actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac ctgggcgtag
     4081 atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt tcccagcaca
     4141 ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg ataagaaccg
     4201 cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc aggtagcgcg
     4261 ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt ttgccgaaat
     4321 tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg cagcggcaag
     4381 gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt gcttggtgcg
     4441 cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct taagctcaca
     4501 atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg cgcctgctcc
     4561 gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc atcgtcgatg
     4621 tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg ttgataattt
     4681 ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc ttcagtgagc
     4741 tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg aagtctgtca
     4801 actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt ttgaataagc
     4861 tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt gatagggatc
     4921 gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat caacaaaatc
     4981 aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac gtaagttaaa
     5041 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     5101 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccggttcag
     5161 tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga
     5221 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     5281 ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc agataaggga
     5341 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     5401 acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg
     5461 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     5521 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     5581 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg
     5641 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt
     5701 taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg cgttttgtga
     5761 tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata tcacacaaaa
     5821 gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     5881 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     5941 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     6001 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
     6061 gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg
     6121 atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
     6181 aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg
     6241 ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
     6301 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
     6361 gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt
     6421 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
     6481 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
     6541 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc
     6601 ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt
     6661 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt
     6721 atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc
     6781 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
     6841 gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct
     6901 aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg
     6961 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
     7021 atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac taacgacctg
     7081 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc
     7141 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac
     7201 agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc
     7261 gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac
     7321 agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac
     7381 gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc
     7441 aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt
     7501 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
     7561 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
     7621 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
     7681 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca
     7741 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
     7801 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
     7861 ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc
     7921 ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca
     7981 ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat
     8041 ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag
     8101 ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc
     8161 tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac
     8221 cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa
     8281 acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg
     8341 cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg
     8401 ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc
     8461 ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga
     8521 taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct
     8581 gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta
     8641 cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg
     8701 cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt
     8761 tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta
     8821 tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga
     8881 ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat
     8941 tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccacagg ctcaggaact
     9001 ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga
     9061 tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg
     9121 gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg
     9181 gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg
     9241 cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt
     9301 caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc
     9361 gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt
     9421 aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac
     9481 gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac
     9541 tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca
     9601 atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag
     9661 ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc
     9721 ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac
     9781 ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga
     9841 tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga
     9901 acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc
     9961 ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc
    10021 ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg
    10081 aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag
    10141 ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc
    10201 catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg cctggacgcc
    10261 atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg
    10321 gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa
    10381 aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgcgc aattaccaaa
    10441 aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac
    10501 ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt ttctaagact
    10561 acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg gcttgcctta
    10621 gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaacaa
//
LOCUS       DAZPFP010000128        10623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPFP010000128 DAZPFP010000000
VERSION     DAZPFP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.128"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..815
                     /locus_tag="ACHUR4_002984"
     CDS             <1..815
                     /locus_tag="ACHUR4_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1456479.1"
                     /translation="NAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIYLLPLAR
                     GNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIGI
                     EAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHUR4_002985"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHUR4_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1456480.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /locus_tag="ACHUR4_002986"
     CDS             complement(1944..3404)
                     /locus_tag="ACHUR4_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1456481.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHUR4_002987"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHUR4_002987"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1456482.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHUR4_002988"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHUR4_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1456483.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6603..7505)
                     /gene="fimH"
                     /locus_tag="ACHUR4_002989"
     CDS             complement(6603..7505)
                     /gene="fimH"
                     /locus_tag="ACHUR4_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1456484.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7525..7968)
                     /locus_tag="ACHUR4_002990"
     CDS             complement(7525..7968)
                     /locus_tag="ACHUR4_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1456485.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8041..8571)
                     /gene="fimF"
                     /locus_tag="ACHUR4_002991"
     CDS             complement(8041..8571)
                     /gene="fimF"
                     /locus_tag="ACHUR4_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1456486.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8581..>10623)
                     /gene="fimD"
                     /locus_tag="ACHUR4_002992"
     CDS             complement(8581..>10623)
                     /gene="fimD"
                     /locus_tag="ACHUR4_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1456487.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2531 a         2761 c         2634 g         2697 t
ORIGIN      
        1 gcaatgccgc taataaccaa ttcaatacaa ataatcactt ccttcatcat ccaaaagtcg
       61 acagtgagtt aacacgtcga tatgaatatg cacgcctgga tacggaaaat atctatcttc
      121 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 aacttaatat tggcaaacag tcctggccat ttaattatgt gacagataca tgcagagaat
      241 tcaatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgctacagag ttagcagaag
      361 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttcctggtta tgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaagaattg
      541 atatctatat tgatcagata ataactgtct caggggtagc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgaga tgggaaatga cccaatcatt ggtatagaag
      661 cctatcctgg taccgcggag atccattcaa aaatgggata taacgttatc cccggtgatg
      721 aagatgcgcc actcaaacgc atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaatggaat tatattggta aatagccact gagaagaatg agtactaata
      841 ttcaggttat acagatattt aaacaaaacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
      961 cgtcgacttt atccagcggc catgaatcaa agagatagcc gtcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
     1201 tttcccgcca ctgccaggac tgacggaata actccaccag catgctgtta tgcgttgctt
     1261 cagcaatggc gagatggaac tgcatgtcac cgctttcgct gctacccggc gcgctggaag
     1321 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcagtgc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     1441 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
     1501 agctatcaag cacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgtggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtgac agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     1861 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccga cgtcaccgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     2161 tcatccacgc cctgggtgta acccatccag ccagccacgc ccagcgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc gggtccagta gacgctgcgg taacttctgg
     2281 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     2461 gcaatggttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     2521 agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg tgtgcccact
     2581 ttatcccagt ccgggcgacc attaacgaag ttatcttcaa tcacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     2761 tcaatccatg ccgctagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     2821 gccacatggc cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     2881 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     3001 tcagcgcagt agcctttttc cgtgaccgtt agagagacaa tcgccgtttg cggacgcgcc
     3061 atcgcgttga gaataccttc gcagccgtcg atttccggat gcagcgcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctttctgcg cctttttccg ctacggtgta cagcagttgc
     3181 tgttttttca ggttttcgat cagcatgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 atgccccagt cgctgtcggt gctttccagc agatgatggg tatacagcgc ctggtgcgcg
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     3481 ggtattaacg gataaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3541 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccactttgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     3841 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcaa gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc agcaactgga ataatcgctt
     4021 tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagctcca
     4081 ggtgcttacg gaactggtcg agggtatacc cttcttccgc gcccggcaga ccggcaatga
     4141 tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt tcagctgcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccacactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     4501 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgcggaacct tggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa ccagaattct gaaattggtt aaccgcgtcg caagaatttc
     4861 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4981 acggttcaac caagtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5041 cggcattggc ctgatgctga tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gttggtggcg gcgctgtccg tcgggatgct ggcaggcatg gatttgatgt cgctgctgca
     5161 caccatgaaa gcgggcttcg gcaacacgct gggagaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cacatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gccttatttt
     5341 cggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
     5401 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5521 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5761 agatacccct gcctgggaag tggttaactt tattggttcc tcgccgattg cgatgtttat
     5821 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
     6061 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatccttgat ccagcaaccg gtcagctggt tggcgtgaat ccggcgctgc tggtactggc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaatgat gcatctttct ggttgttcaa
     6241 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
     6361 cagagcctgc caatggcagg ctcagactga tgactaaagt ctaaaaatgt gcccggacag
     6421 tcccctcgcc cctccgggga gagggttagg gtgaggggaa cagaccagta aagtgcgctt
     6481 tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag
     6541 gttttgtagg tctgatagcg tagcgcctca ggtaccagca ttagcaatgc gctgtaattt
     6601 ttttattgat aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc
     6661 cctccggtac gtgcgtaatt tgccgttaat cccagactta ccgccgaagt tcctactgct
     6721 cctaacgata ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg
     6781 acgccctgcg ctggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg
     6841 gttgtgccgg agaggtaata ccccaggttt tggcttttcg cacaataaac ggtaagagga
     6901 attggcactg aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatca
     6961 caaccgccag tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa
     7021 tcatcgctgt tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct
     7081 ttaatagcca ccccgcccgc actgctcaca ggcgtcaaat aaagcgccac cggccacggc
     7141 ttatccgttc tcgaattata aacaacccgc ggcgtttcgc tggtggtcgg aaatggatag
     7201 ctactgccac tatattttac ggtcccggaa aaattagata acacgccgcc gtaagccgag
     7261 cctcgttgca gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt
     7321 tgcgtcgaaa gatctacgac caggttttgc cccacattca cgacaggcgc aaggtttaca
     7381 taaacattag cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg
     7441 aatgaccagg catttaccga ccagcccatc agcagtacag caaacagggt aatagctcgt
     7501 ttcattacaa ttatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg
     7561 aatggttccc tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg
     7621 cgctgattgt gaggaatcat ccacctgaac tgttttggtt gcgccagtat tcaatgtgtt
     7681 gccactgtca tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata
     7741 tccggtgctg tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca
     7801 attagtcaac tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact
     7861 gaaagaataa agatcgccga gatctaccgt ggcattggtg gtggaaaccg tacacggttt
     7921 ggcgacgacc ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc
     7981 gagcgccaac attgccgcca atacataccc acgtttgcac catttcatga gcatctccag
     8041 ttactgatat tcaagagtga aggtagccgt agcattgata tgccccgcag tgacaggcac
     8101 ctgtgtcgcc attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt
     8161 cgtccacgaa atcgcagacg atggagcatt aaggggtatt tgattttgct gctcattcag
     8221 aagctgtatt cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc
     8281 attgtggcta tctgcaacgc cggtaaaccc aacttttacg gcagaaacgg cattaccaca
     8341 gggtgacagc aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg
     8401 cttcgccgcg ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca
     8461 gccgttatct ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aaacgcgact
     8521 taccgccagc cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc
     8581 ttaacgacat tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg
     8641 ataattggcg acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc
     8701 taaaggcatt ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct
     8761 actctctgat gtcaccatcg ccccaaacgg cagcggctta ttattgtgag ttagcgtcat
     8821 gagcagtttt atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg
     8881 aacaacgtta gcgaccgcgt tatctaaatc gacgttatca gccaaggtat tggtatccag
     8941 cgccactcta ttttcccgat attcagtggc ataaggcagc acggcataac cacgccagtc
     9001 ggtacgcacc cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag
     9061 caccaccgta tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccaatacccc
     9121 accgctgact ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatattggc
     9181 attgccgtaa ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc
     9241 gcctcccccg gcatagccgg tttgcacgct ataactgagg ttgttgtctt ccagcaacgt
     9301 accgtataca ccagccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtagct
     9361 ggcactggca tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat
     9421 attgacgtta agcgctaaca tctgatcccg tcctttttgc caggcgtttt tcgtcaggct
     9481 atagctgagc gtccagttga tatcttcgaa cgcagtattt aatccagcct ggaattgctc
     9541 atcgacatta ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatgt
     9601 gcgcccgagt tgctgagtaa cggtgagctg taatttcccg cgtttgttat aagcgaggtt
     9661 gtaatagtcg gtgaatttcg gcttaacctg aataactcca tcctgtgttt cgatgttgta
     9721 gccattcatt cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg
     9781 gtaacccact aactgaatat tcgtgcctga ttcattgagc gatttgttat agagaaaacg
     9841 caccgattgt ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc
     9901 cacagacaga gcgcccagtg cctccatgtt tttcccgata ccgaaattaa aagcacgata
     9961 acgatccgcc agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa
    10021 tgtactctgg aaaaagcggg gtttttcctg ctgcgcattt ccactacggt attctcctgc
    10081 cgtaatggaa taacgagtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac
    10141 ggtaaaaatc tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt caccactatt
    10201 acctgcggca tagatatcgt tgatggtaaa aggccccggt ggcaccgtac tattataaat
    10261 gccatatcca ttttgtttaa tagtgacctg tgcagtacca cgagcaatac cgtggatcac
    10321 cggggcaaat cctctttggc tatcgggtaa catattgtca tctgaggcca attgtgcgcc
    10381 gcgaaagtta ataccatcga aaatatcgcc ctgggtataa ccatcaccca acgtcagccg
    10441 ggaacgtaaa ggaattatgt ctcgctcaat ccaggtaatg atatgctgcc atttattttt
    10501 gctacctgat gaactgtcgc tactgttata actccaggtg gtattgtcgc gtaaacgcca
    10561 cgcaccaata tttaacccac tctgtaggtt taaatatgca taatggctgt tacccccaat
    10621 ccg
//
LOCUS       DAZPFP010000129        10464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPFP010000129 DAZPFP010000000
VERSION     DAZPFP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.129"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHUR4_002993"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHUR4_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1456488.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHUR4_002994"
     CDS             213..401
                     /locus_tag="ACHUR4_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1456489.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHUR4_002995"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHUR4_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1456490.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHUR4_002996"
     CDS             596..769
                     /locus_tag="ACHUR4_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1456491.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHUR4_002997"
     CDS             complement(818..1891)
                     /locus_tag="ACHUR4_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR1456492.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUR4_002998"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUR4_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHUR4_002999"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHUR4_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1456493.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUR4_003000"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUR4_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1456494.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHUR4_003001"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHUR4_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1456495.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHUR4_003002"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHUR4_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUR4_003003"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUR4_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1456496.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZPFP010000130        10417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPFP010000130 DAZPFP010000000
VERSION     DAZPFP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.130"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..695
                     /locus_tag="ACHUR4_003004"
     CDS             <1..695
                     /locus_tag="ACHUR4_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456497.1"
                     /translation="SFIQSEINKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGSH
                     KTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSDNKIQGVGSFMSWS
                     FLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSI
                     CSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEM
                     NAQLDYLNRYAT"
     gene            988..2229
                     /gene="agp"
                     /locus_tag="ACHUR4_003005"
     CDS             988..2229
                     /gene="agp"
                     /locus_tag="ACHUR4_003005"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1456498.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2267..2494)
                     /locus_tag="ACHUR4_003006"
     CDS             complement(2267..2494)
                     /locus_tag="ACHUR4_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1456499.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2515..3111)
                     /gene="wrbA"
                     /locus_tag="ACHUR4_003007"
     CDS             complement(2515..3111)
                     /gene="wrbA"
                     /locus_tag="ACHUR4_003007"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1456500.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3484..3591
                     /locus_tag="ACHUR4_003008"
     CDS             3484..3591
                     /locus_tag="ACHUR4_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR1456501.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(3673..5001)
                     /gene="rutG"
                     /locus_tag="ACHUR4_003009"
     CDS             complement(3673..5001)
                     /gene="rutG"
                     /locus_tag="ACHUR4_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1456502.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(5022..5516)
                     /gene="rutF"
                     /locus_tag="ACHUR4_003010"
     CDS             complement(5022..5516)
                     /gene="rutF"
                     /locus_tag="ACHUR4_003010"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1456503.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(5527..6117)
                     /locus_tag="ACHUR4_003011"
     CDS             complement(5527..6117)
                     /locus_tag="ACHUR4_003011"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1456504.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(6127..6927)
                     /gene="rutD"
                     /locus_tag="ACHUR4_003012"
     CDS             complement(6127..6927)
                     /gene="rutD"
                     /locus_tag="ACHUR4_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1456505.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(6935..7321)
                     /gene="rutC"
                     /locus_tag="ACHUR4_003013"
     CDS             complement(6935..7321)
                     /gene="rutC"
                     /locus_tag="ACHUR4_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1456506.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(7333..8025)
                     /gene="rutB"
                     /locus_tag="ACHUR4_003014"
     CDS             complement(7333..8025)
                     /gene="rutB"
                     /locus_tag="ACHUR4_003014"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1456507.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(8025..9116)
                     /gene="rutA"
                     /locus_tag="ACHUR4_003015"
     CDS             complement(8025..9116)
                     /gene="rutA"
                     /locus_tag="ACHUR4_003015"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR1456508.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            9404..10042
                     /gene="rutR"
                     /locus_tag="ACHUR4_003016"
     CDS             9404..10042
                     /gene="rutR"
                     /locus_tag="ACHUR4_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1456509.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(10336..>10417)
                     /locus_tag="ACHUR4_003017"
     CDS             complement(10336..>10417)
                     /locus_tag="ACHUR4_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456510.1"
                     /translation="TGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         2625 a         2706 c         2676 g         2410 t
ORIGIN      
        1 tatcttttat tcagagtgaa attaataaaa catatggatt aaaatacgct ccagtagatg
       61 gcgatggata ttgtctgtta agagccatac tggttttaaa agaacatgag tattcatggg
      121 cgctgggcag ccataagacg caaaagcaag tatacgaaga gtttattaaa attgttgata
      181 aacaaacgat cgaggctctt gttgatacgg cattctataa tctcagggaa gatgtaaaga
      241 cattatttgg cgttgatctg caatctgaca acaaaattca gggtgtgggt agttttatgt
      301 catggagctt tctgtttttt aaaaaacaat tcattgatag ttgcttgaat gacaaaaaat
      361 gcatactgca tttacccgag tttatattta atgataacaa gaatttgctt gctttagata
      421 ccgacacgtc ggataggatt aaagcggtga agaattttct tgctgttctt tcagatagca
      481 tttgctcatt atttattgtt aatagtaatg tggcatcaat ctccttggga aatgaatcct
      541 tttcaacaga tgaagatctt gagtatggat atttaacaaa cactggcaat cattatgatg
      601 tttacctccc tcctgaactt ttttctcagg cttataagtt aaaaaataag gaaatgaatg
      661 cgcaacttga ctatttaaat cgttatgcaa cttaatggca aaggcatatg ctaaaaacca
      721 ttgttattag tctcacactt ttttattggt aaatattgtc tctgtattgg taacgccgca
      781 gatattctgt ttagccacag gtgcaattat cagcggcgta cgcgaggcag gggctaatca
      841 ggcatagttt gcgtcaaacc ttgcctgttt ttgaagatgt atatagaaaa acaggcgttc
      901 aacaagccat tttgcgaacc tgttcccgga aaaaagtcat atttctgtca cactctttaa
      961 tgattgataa caaaagaggt gccaggaatg aacaaaacgc taatcgccgc aactgtggca
     1021 gggatagttt tactcgcttc aaatgctcag gcacaaaccg taccggaagg ctatcagcta
     1081 cagcaagtgc tcatgatgag ccgccataac ttacgtgcgc cgctggcgaa caatggcagt
     1141 gtgctggagc agtcgacgcc gaataaatgg cccgaatggg acgtccccgg tgggcaactc
     1201 accaccaaag gtggcgtgct cgaagtgtat atgggccatt acatgcgtga atggctggca
     1261 gagcagggga tagtgaaatc gggggaatgc ccgccgccgg acaccgttta tgcctatgcc
     1321 aatagtctgc aacgtaccgt cgctaccgcc cagttcttta ttactggtgc attccctggg
     1381 tgtgatattc ctgtgcatca ccaggaaaaa atgggcacta tggacccaac ctttaacccg
     1441 atgatcaccg atgattccgc cgcattcagt gaacaggcgg tggcggcaat ggagaaagag
     1501 ctcagcaaac tccagcttac cgacagctac cagctactgg aaaaaatcgt taactataaa
     1561 gattcccctg cctgtaaaga gaaacaacag tgttcgctgg tggatggcaa aaataccttt
     1621 agcgccaagt atcaacaaga accaggtgtt tccgggccgc tgaaagtcgg caactcgctg
     1681 gtagatgcgt ttactttgca atactacgaa ggttttccga tggatcaggt ggcctgggga
     1741 gaaatcaaat ctgaccagca gtggaaggta ttgtcgaagc tgaaaaacgg ttaccaggac
     1801 agtctgttta cctcaccgga agtggcgcgc aatgttgcga aaccgctggt cagttatatc
     1861 gacaaagccc tggtcaccga tcgcgccagt gctccgaaaa tcacggtgtt agttgggcac
     1921 gactccaaca tagcctctct gttaacggcg ctggatttca aaccgtatca gttgcatgac
     1981 cagaacgaac gcacgccgat tggcggcaaa atcgttttcc agcgttggca tgacagcaaa
     2041 gccaatcgcg atttgatgaa aattgaatat gtgtaccaga gtgcggaaca gttacgtaat
     2101 gccgatgcgt taaccctgca ggcacctgcg cagcgcgtga cgctggaatt aagcggttgc
     2161 ccgatagatg ccaacggttt ctgcccgatg gataagtttg atagcgtgtt gaatgaggcg
     2221 gtgaaataac agaaaactcc cccgcgagaa gcgggggagt cgctggttaa acgtttttac
     2281 gttcgatggt ctgttcgccc caaaaaagcg aatctttatc ggtcttagca aaggctttga
     2341 ctaacacttc atcactacct tcttcccaaa tcttttctgc cattttttcg tcgtacccgg
     2401 cgacttcgaa aatggcctcg gctatttccg gcgacgtatt gcgcagagat gcccattcac
     2461 cgacgtgatg agctttcgct tcttgagttg gcatgcgtat cctcctgttg aagattagcc
     2521 gttaagttta actgccagac ctgcgacata ttccccttga taacgagcaa tagacagttc
     2581 ttcctggctt ggctggcgtg agccgtcacc gcctgcgatg gtggttgcgc cgtacggcgt
     2641 accgccgcga acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa tggggacaat
     2701 caccatgccg tgatgcgcaa gggtcgtcca ggtggatgtg atggtttgtt cctgaccgcc
     2761 gccagtaccg gtggaactaa agacgctcgc cagttttccg tatagtgcgc cggaagccca
     2821 caggccgccc gtctggtcga ggaaggtacg catttgaccg gacatgttgc caaagcgggt
     2881 aggtgtacca aaaataatgg cgtcgtaatc ggccagttct tgcggggttg caaccggtgc
     2941 agtttgcgtt ttaccgcctg ctttttcaaa taattgcggc ggcatggttt ccggtacacg
     3001 cttaacgaca acttctgcgc catccacttt gcttgcaccc tcagcgactg cgcgtgccat
     3061 cgtttcaata tgtccgtaca tggaataata aagcaccaga actttagcca tttctaacca
     3121 ctcctcgtgt tatctctatt ccgtagcgat tcgctaccac ttatttaaag ataagacgtc
     3181 cttttcagag tgcaaatttc acaaccactt atttgattta taacaacttt cacaagcacg
     3241 taattttgtc gcaaaatgac acatttttat ctcatcgcgt ttttttaatc ataagagcgg
     3301 cttatggata attattggag atgatatcta ttctcgctaa gaagctgttg caggatatta
     3361 ccaaacgcgg gtctgtccgc gtcagttcac taagcttagt cccacgtagc gaaaatatgg
     3421 cagccgccat acgccgcgtt aattctatgc aatatgatgt ctatacccag acggaggtca
     3481 gtaatggcaa accatcgagg cggttccggc aattttgcag aagaccgcga aagagcatca
     3541 gaagcaggta aaaaaggtgg caagagcagt cacggcaaaa gcgacaacta gccgggctaa
     3601 tcaatgacga atgcattttt ttcttaagct cgtcaaaaag tcatcaccgc cggtaacccg
     3661 gcggttttct gtttatggct cctgatgaac aacttctggc ggtggaacgt caaccaacct
     3721 tctgcttaat aacgcattga gtaaaatcgc gccaaaggtt gctgtaccga tccctcccaa
     3781 cgtaaaaccg ccaagcgtga gagcaaaatc acccgcgccc agcacgaagg ttactgcgac
     3841 cataatcaaa ttagcgttct ggcttaaatc gacacggttt tgtacccata tccttgcgcc
     3901 tgcgacggca atcagcccga acacaacaat tgatgcacca ccaatcaccg cggccggaat
     3961 ggtatggatc agcgcgccaa atttcggcga aaagcccaac agcatagcga tgacggcagc
     4021 tgcaacaaac accagcgtcg agtagacttt tgtcacggcc atcacaccga tattttcagc
     4081 ataggtggtg acgccgctac cgccgacaga gccggaaagc atcgttgcca gaccatcacc
     4141 gacgaatgct cgccccatat acgggtccat attgcgtccg gtcatcccgg cgaccgcctt
     4201 gagatgacct aagttttccg ccaccagaat caccgccacg ggcgcaatca acatcatcgc
     4261 ctggccatta aaaccaggag tggaaaaatg cggcagaccg aaccaggcag catagctgac
     4321 gagagtaaaa tcgacggctt ttcccagccc taaaacgttg gtcatcacgc catacagcag
     4381 acaggcgaca attaatccta cgagaatcaa taaccgctgg atcatgccac gggtaaacac
     4441 cgccaccagc ccaatacaca gcaccgtcat taccgccatc cagctatcaa aggccgaagc
     4501 cgatacactt ttcactgcga taggcgctaa gttcaggcca atcgccatca ccaccgcacc
     4561 cgtcaccacc ggcggcatta gtcgttcaat ccagcgtgta ccaattttca tcaccaccag
     4621 gccaatgacg gtataaacca gcccacaggc gataatcccg cccagcgcga tgctgatatt
     4681 cgggttaatg ccctgaccgt taaagcccgt cgcggcaatc accacgccga caaaagccgc
     4741 gctggagccg agataactgg ggacgcgccc gccggtaata aagaaaaaca gtaacgtgcc
     4801 gatccccgac attaaaatgg aaagattggg atccagcccc atcagaatcg gcattaacac
     4861 cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt actgccgtct gagcaaacgg
     4921 caatcgttca tccggcgcga ccacgccgct ctctgtagag gtcgatttta actgccagtg
     4981 aggaaaaccg aacattgcca tcagctgtct ccttaaggag gttaacaagc agggcgcatc
     5041 agcgcgtgat aactgcgatc gaaccacacc agcccgtagg gtgtggtgtg acgatgaatc
     5101 gcttcgatgg cgcaaaacag aatgtcgtgg gtgccgacgc tcaccacctg gctgatacgg
     5161 cagtcaaacg aaaccagagc ctcttccagt tgcgggcatc cggtcacacc cgtctgccag
     5221 cgggcggcgg caaagcggtg ttccatgggc gttttgccgc caaaaagatt tgaaagcggc
     5281 tcctgcccgg cgctcagtgt atttacacac agcgttcgat tttcattgaa taccggccag
     5341 acggacgccc cacgattcag gcacaccagt aatgtgggcg gcgtgtcggt cacgctgcag
     5401 acggcgctgg cggtgaaccc ggcgcgcccg gctggaccgt ccgtggtgat gatattgacc
     5461 gccgcgccca tacaggacat cgcatcgcga aaggtttgtt gatcaactat gttcatagtt
     5521 tgctccttac aacagcccgc aggcttcttc aaaggacaga cgtggcaggc gcgcaaaaag
     5581 tttgctgcta tcgccatagc cgatattaat cagcagattg ctcttcagcg tgctgcccgc
     5641 aaaaaaggcg tcgtcaacgt gttgacggtc aaaacccgac atcgggccgg tatccagtcc
     5701 cagcgcacgg caagcgacga tcagataagc cgcctgcatg gaactgttgc gaaacgctgt
     5761 ttcttcggca agttgtgggc tggaggtaaa ccaactgcgg gcatcaccgt ggggaaacag
     5821 tagtggtaac cgttcataaa attcactgtc ccaggcgacg atagcggtga cgggcgcggt
     5881 cagggttttt tgcagattgc cgctggaaag cgccgggcgc agacgttctt ttccttctgc
     5941 agtgcggata aacacgatcc gtgccggaga acagttagct gatgtcggcc cccatttcat
     6001 cagggcataa agctcctgta acgtctcatc gctgacgggt gtctcccgcc agccgttgtg
     6061 agtgcgggca tcggtgaaca gggtgctaag cgcacctggg ctaacggctt cgttcatagc
     6121 aattccttac agggcggctt cacggtgatg taacaggctg gcaagcccgt tgagtaacag
     6181 agcattaaac gtttcgggat cggtcacgtt gcaggcgtgt ccgccatagg gcatcaccat
     6241 tttctggcta tcgggcaggg cggcatgaag ttcactggaa catgctgttg gcaccagcag
     6301 atcatcactg gcgcagatga tttgcaccgg gcagcggatg cgatccgcat ggtgactaaa
     6361 gtcagcgcgt ttgagggcgt taagtcgacg tagtaaatta tttttgccct gaaaatgcgc
     6421 cagtgccagc gcgtcttctg cctccaggcg aggtgcgcgg gccgccatcc agtcggcggg
     6481 atagaggaac aacggctgcg cttccaccca tgcttgcgcg ccgccgctat acagtaatcg
     6541 ttcgcgaacc tgaaaacagc ggcgcgtatg ggcgtttatt cgtagccagc cgttaacgct
     6601 gaccagcata gttaccgacg cgggataatc cagcgccagc tgcatcccca ccagcgcacc
     6661 gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg gctaccagcg cctgatgcag
     6721 ttccgctgcc atctgggcga tactgtaatc ttctgccagt gtgtcgggat tattgccggt
     6781 gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc agcaccgcca gttgcggtaa
     6841 ccagtaactg ccgctaccgc caagacccga aatcaacacc actacgggtg catcagcata
     6901 agggggagat gagagtgaaa gtttcatcgc ggcctcactt ggcgatatgc gcaattgtgg
     6961 cgatttccac cagcgcgtca ggttttacca gtccgcactg aatgtagaat cgcgccggtt
     7021 tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc ggcgtaattt ttccagtcgg
     7081 taataaagat gctgttgaag gtcacatccg ccatcgtgcc acccgccgtc tcgatcacct
     7141 tgcggatagt ttccagaacg tggcgggttt gcgcctttgg gtcatcggca aacagcacgt
     7201 tattatgttg atcaaaagcc agcgtaccgg agacatacac cacgccatca gccagcgtgc
     7261 cgggaacgaa gggggccagc ggtgcgctgc tgccagcggg aataattacg gattttggca
     7321 tcgctaaact ccttaagcga tacgagcaaa ggacgtggga gaaagcgcgt cgcagaacgt
     7381 ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg aacaacgcgg ctttctgtgc
     7441 aaattccggc cccgcctggt gtgttgcgtc ttccagcacc acgccgaaat gctccagaaa
     7501 aaagccatcg cgtagcgtcg attcgacgca gacgttggta gcgataccgg tgaaaaccag
     7561 atggcgtatt ccacggctgc gtaaaatgct gtccagcggc gtattgaaga aaccgctgta
     7621 gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc agttcatcca ccagttgata
     7681 atcccaggag cctttcgcca gcaatttccc ctgcagctgc ggctgcttac gcatggtttt
     7741 cagggcgttc gatttatgaa aattcggtga gccggggccg ccagcctcga catactgttc
     7801 atcccagcca ttttgaaacc agatgatcaa catccctgcc gctcgcgctg cggtcacagc
     7861 ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca tcaaacccgg cgagatctaa
     7921 gtagccgcct ggcgtggcat aagcgttttg catatccacc acgatctgcg cactttgctg
     7981 cggatcgaag gtaatggctt ccggtcgagc ggttaaggtt gtcatcatgc cacctcctga
     8041 gtcagcgcag agagatgggc gcggcactgc atcagtggtt gaatgcgctc gccgaaggtt
     8101 tcgattcccg acagaaaatc atcgaaggtc aacagcacgc cttctgcacc aggcacgctt
     8161 gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac cgactaacgt ccccatattg
     8221 atgtttaccg ccgaagtggg atcggccatc tgacgaacgt tggtgtcagt accggagcgg
     8281 gtatctttct gactttgttc ggttagccag cttaacgcct cttcatccgc gcccgctttg
     8341 tagtgttccc atttggcgcg agtggcatcg tcggtttcat cggcaatcac cataaacaac
     8401 acataagagc caacgtcgcg tccggtttgc tctgtggcct gtttcatccg cgcagcggtc
     8461 ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac agaagttgaa atcggcatac
     8521 tgggcggaga acgccatgcc agcgtcgctt tgcccggcgc agatcacttt catggggata
     8581 ctcggttgcg gactgacgcg acaatcattc atggtgaaaa aatcgccttt aaaatcgctt
     8641 ttccccgtgc cccacaggtc gcgcagcacc tgaacatatt cggtgagata gtcgtaacga
     8701 cgggagaaat agtcatcgcc aggccagata cccatctgct catactcggg cttttgccag
     8761 ccagtcacga ggttgacgcc aaaacgcccg ccagagatgg agtcgatggt tgcggccata
     8821 cgggcgacga ttgccggagg taacgttaag gtggcagcag tggcgtaaat ctgaatgcgc
     8881 gaggtcacgg ctgccagacc cgccatcaag gtgaacgact caaggttgtg atcccagaac
     8941 tcagttttgc cgccaaagcc acgcagtttg atcatcgaca gggcgaaatc gaaatggtaa
     9001 tgctccgctt tttgcgcaat ggctttattc agttcaaagg tcggcatgta ctgcggcgcg
     9061 tgggtcgaaa tgagccagcc gttgttgcca ataggtacga atacgccaat tttcatcatc
     9121 aacctctctt cgtctcgtaa agtgaaagtc agacggggcg ctgcatcctg catatccttt
     9181 tcagccgcgt attggcttgt tcgcaaagcg gatgccagtt tttaaaaagt gaatattatt
     9241 aatgtgttag cattgtgtta tctaaaatat ctggtaaaaa gtggactaaa cggtcaaaac
     9301 agttgcacat aaaacatgca tccgtgcgcg atgagagtgc agaaggtcga ggccgggcgg
     9361 gggttttgct atcctgttac caatctacaa gaggggagag cgcatgacgc aaggcgcagt
     9421 gaaaacaacg ggtaaacgtt cgcgcgcagt aagcgcgaag aaaaaagcga ttcttagcgc
     9481 agcactggac actttttcac aattcggttt tcacggcaca aggctggagc agatcgcaga
     9541 gttggcgggt gtttcaaaaa ccaatctgct gtattacttc ccgtcaaaag aggcgctgta
     9601 tattgccgtg ctgcggcaga ttctcgatat ctggctggca ccgttaaaag cgtttcgtga
     9661 agatttcgcc ccgctggcgg cgataaaaga gtacatccgt ctgaagctgg aagtctcacg
     9721 cgattatccg caggcttcgc gcctgttctg tatggagatg ctggcaggcg cgccgttgtt
     9781 aatggatgaa ctgacgggcg atttgaaggc attaattgat gagaaatcgg cgctgattgc
     9841 cggttgggtc aaaagcggca aactcgcgcc gattgatccg cagcatttga tttttatgat
     9901 ttgggcttcc actcaacatt acgccgattt cgcccctcag gtggaggcgg tgacaggcgc
     9961 gacgttgcgc gatgaggtat ttttcaatca aacggttgaa aacgtgcagc ggattattat
    10021 tgaggggatt cgaccacgtt aaagatgacg gaggaggttg taacatcctc cggctacctg
    10081 tttaaccgat cgtcattaaa ctggcgttac cgccagcggc agcggtattc acactcagcg
    10141 aacgttcatc gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg
    10201 tttcttcaac accacttttg ctccatatga acggaaccga cgattaaact ggatggctct
    10261 gattgattca gggtatgaat ggcggttttt tgctccgttt ccctcaaaat ggacgcaact
    10321 tcccctctgc ggctctcagc cgcaccaccg catccgggcc agcggctcat gcatcaggac
    10381 ctgctctgcc agacggtagc cccgcttcag ccccgta
//
LOCUS       DAZPFP010000131        10284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPFP010000131 DAZPFP010000000
VERSION     DAZPFP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.131"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..523)
                     /gene="fliZ"
                     /locus_tag="ACHUR4_003018"
     CDS             complement(<1..523)
                     /gene="fliZ"
                     /locus_tag="ACHUR4_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1456511.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKS"
     gene            complement(569..1288)
                     /gene="fliA"
                     /locus_tag="ACHUR4_003019"
                     /pseudo
     CDS             complement(569..1288)
                     /gene="fliA"
                     /locus_tag="ACHUR4_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(1453..3105)
                     /gene="fliC"
                     /locus_tag="ACHUR4_003020"
     CDS             complement(1453..3105)
                     /gene="fliC"
                     /locus_tag="ACHUR4_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR1456512.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            3371..4777
                     /gene="fliD"
                     /locus_tag="ACHUR4_003021"
     CDS             3371..4777
                     /gene="fliD"
                     /locus_tag="ACHUR4_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1456513.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            4802..5212
                     /gene="fliS"
                     /locus_tag="ACHUR4_003022"
     CDS             4802..5212
                     /gene="fliS"
                     /locus_tag="ACHUR4_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1456514.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            5212..5577
                     /gene="fliT"
                     /locus_tag="ACHUR4_003023"
     CDS             5212..5577
                     /gene="fliT"
                     /locus_tag="ACHUR4_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1456515.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            5655..7142
                     /gene="amyA"
                     /locus_tag="ACHUR4_003024"
     CDS             5655..7142
                     /gene="amyA"
                     /locus_tag="ACHUR4_003024"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1456516.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7176..7589)
                     /gene="yedD"
                     /locus_tag="ACHUR4_003025"
     CDS             complement(7176..7589)
                     /gene="yedD"
                     /locus_tag="ACHUR4_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1456517.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            7776..8981
                     /gene="yedE"
                     /locus_tag="ACHUR4_003026"
     CDS             7776..8981
                     /gene="yedE"
                     /locus_tag="ACHUR4_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1456518.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            8978..9211
                     /gene="yedF"
                     /locus_tag="ACHUR4_003027"
     CDS             8978..9211
                     /gene="yedF"
                     /locus_tag="ACHUR4_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1456519.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9318..9988
                     /locus_tag="ACHUR4_003028"
                     /pseudo
     CDS             9318..9988
                     /locus_tag="ACHUR4_003028"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10028..>10284)
                     /locus_tag="ACHUR4_003029"
     CDS             complement(10028..>10284)
                     /locus_tag="ACHUR4_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1456520.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2504 a         2613 c         2599 g         2568 t
ORIGIN      
        1 aggatttctg cacaagtccg gtacaggttt ggcgctgata gtgttgatac ttccgtaatg
       61 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
      121 aaccgtcctg cagctgatcg agggaaatat tttgctcatg tagatgatta cccaaacggc
      181 gcagacgaac gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa
      241 atttcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
      301 aaaatcggca caatgccgcc catgattgtt gttccgtttg ccagcctttt tcatcaagca
      361 gcgtgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga actaaggtaa
      421 tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa
      481 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
      541 caaagttaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
      601 taatgtcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
      661 tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg
      721 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta
      781 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcac
      841 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcagcatt tgacgataat
      901 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
      961 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
     1021 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tacactgcgt
     1081 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacagcatta agtaacccaa
     1141 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
     1201 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
     1261 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
     1321 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
     1381 atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag
     1441 gcattcagac gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt
     1501 tgccagcacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc
     1561 ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt
     1621 gttcaggttg gtgactgcgg aatccaaacg gttttgcacc gcacccaggg aggagcggaa
     1681 tttgtcgatg gagctgatgg cttcatccag agctttcagg ggatcggctg tcgtagctgc
     1741 ttttgtttca gcatctgtcg tcaatttacc atcagcatct ttataaactg ccttaccgct
     1801 gccgttagtc actgaaccat catcctgtag gtaaaaatct acagtatcag atgtggtcaa
     1861 cgcctcgttg ttatcaacat aaaccgaatc aacacctttt gttacagcgc cagttgtgcc
     1921 gttgacgcta taacctgcag tagtgaaatc aacattgttt cccgaggttg atacagcttt
     1981 atcgaaagct gcctgatcaa cttttgcacc tgttactgaa taagtaatag tgtccggcgt
     2041 agtactatta ccggtaaagg agatagaggt accgccaatg tcaatggttg tcgctttggt
     2101 attattggct gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag agccgttttt
     2161 cgttaagcgc ccagttgtat caatgtagag tttatctcca ttgcttgccg taattttacc
     2221 atcactgctg atctgaacat cctgtgcaga accatcgata gtaatagtgc ccttagcggt
     2281 atcaccaact cccggggtca aatatttctg aacatcggca gctgaagccg ttgtggtatc
     2341 aaaagagtat gacttacttg cactgtcata cttgtaattc gttgcactag ccgccgttcc
     2401 gaagccgtta ttaatggttg cctgaacagt atcaccatca ctaactccag ccagcaaatc
     2461 agacgcttta gcagcatcgt aacccgcact cacagtatat ttgttgccga ccactgttgc
     2521 attagcagct accaagtcag ctttagatgc agcagtatta gccacagccc cgccaccatt
     2581 cacattaaac ccattcagcc ccagcgtatc agagtcaatt ttcttcagat caatagtgat
     2641 agtctggccg tcattcgcac caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt
     2701 cacgccgttg aactgggtct gaccggatac gcggtcaatt tcgtcgagac gggatttgat
     2761 ttcgtcctga atggagtcca gatccgaatc agagttagtc ccggtagaag cctgaaccgt
     2821 cagctcacgg atacgctgta agttgttgtt gatttcggac agcgcgcctt cagtggtctg
     2881 tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt taatgttaga
     2941 agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa
     3001 gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt tgatattatt
     3061 ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc gttatcctat
     3121 attgcaagtc gttgattacg ttttgggttt ccacccgtcg gctcaatcgc cgtcaaccct
     3181 gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca ttttttgtta
     3241 gtcgacgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa caattaaacg
     3301 taaactttgc gcaattcagg ccgataaccc cggtattcgt tttacgtgtc gaaagataaa
     3361 aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg atttaagttc
     3421 aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt caaatcagca
     3481 atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt
     3541 ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca gcaccaccag
     3601 cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga aatacaccat
     3661 cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca gagatgatac
     3721 gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg acgacaaaga
     3781 tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat
     3841 caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg gtgaatatcg
     3901 tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct cggtcagcgg
     3961 tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca acggtatgga
     4021 ggtctcggtc gccgcccaga atgcgcagct gacggtcaac aacgtcgcca tcgagaacag
     4081 cagcaacact atcagcgacg cgctggaaaa catcactctg aacctgaacg atgtcaccac
     4141 gggcaaccag acgctaacca tcactcagga cacctccaaa gtgcaaacgg cgattaaaga
     4201 ctgggtgaat gcctataact cgctaataga taccttcagc agcctgacca aatacaccgc
     4261 cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg gcgactccac
     4321 gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca gttcttccag
     4381 ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca aactggaact
     4441 ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat
     4501 tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc tgaccagttg
     4561 gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaaa ccctgaataa
     4621 gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa
     4681 agaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca gcagctactt
     4741 aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg ggagacaggt
     4801 catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat
     4861 gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt
     4921 tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa
     4981 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga
     5041 attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa
     5101 tttacgcaac gatgtctcag cagtcgaaga ggtggaagca ttaatgcgta atattgccga
     5161 tgcctggaaa gagtctttac tctccccttc ttcgattcag gacccagtct gatgaacaat
     5221 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
     5281 ctggcaacgg aagaacaatg ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg
     5341 gtgcaggaga tcgcacacct gactgaagag gttgccccgt ccactacgat gcaggagcag
     5401 ctccgcccga tgctgcacct gattctcgac aacgaaagca aggtaaagca gttattacag
     5461 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
     5521 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
     5581 ttaatgagcc gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg
     5641 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
     5701 aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta
     5761 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct
     5821 acgactccta tgatttattt gatctaggtg agtttgatca gaaaggcagc atccctacta
     5881 aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg
     5941 cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
     6001 gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg
     6061 aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg
     6121 atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta
     6181 aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta
     6241 atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga
     6301 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg
     6361 cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta caggaagttg
     6421 cgccaaagcc gctgtttatt gtggctgaat gctggtcgca tgaagtcgat aaactgcaaa
     6481 cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat
     6541 tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc
     6601 tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt
     6661 tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
     6721 tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattacg
     6781 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
     6841 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc
     6901 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
     6961 tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
     7021 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgacagac gaaaacggcg
     7081 aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
     7141 aaatccatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
     7201 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
     7261 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
     7321 acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat cccccgctgc
     7381 cactgacggc aatccaaggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc
     7441 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
     7501 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
     7561 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
     7621 gtggcattaa agctctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
     7681 tattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcggctc
     7741 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
     7801 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
     7861 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
     7921 gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
     7981 tccatctgga aggatcgcca ttaacccgga tcgacggcat gatgatcctc ggtatgtttg
     8041 gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc
     8101 gtatccgcat tatgcaggcc atcattggcg gcattatcgc cggttttggc gcgcgtctgg
     8161 caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct
     8221 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
     8281 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
     8341 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
     8401 gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg
     8461 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
     8521 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
     8581 ccatcgggat cttcagttac gtacagttag gcgttgaacc taaaatcatg tgggcgggac
     8641 caaatgcggt aattggtggt ttactttttg gttttggcat cgtgctggca ggcggctgcg
     8701 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
     8761 gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
     8821 ccaccgactg ggacaaaatc aacctgctga aaacttttgg tccgatggga ggcctgctgg
     8881 tgacatattt gctgctgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct
     8941 tccgccgtga ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
     9001 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
     9061 gccgcaatta aaaaaaggtg agatcctgga agtggtgagc gactgtccgc agtcgatcaa
     9121 taatattcca ctggatgcgc gtaatcacgg ctacacggtg ctggatattc agcaagacgg
     9181 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
     9241 gaacatcgtt acctctgaca catagatttt acccgccccc tctcctattc ttttcagtat
     9301 gatctcagga ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc
     9361 ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca
     9421 gatacaatgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc
     9481 atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta
     9541 acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa accgctctgg caacatggtc
     9601 gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc
     9661 ttattttatc tatcgcgctg acggacaacc tgtttttatg gccgcgatag gtagcatacc
     9721 gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg
     9781 tctggtagat attcatgacc gccgcccgct ggttctgtcg ccagaagctg cacgggaatg
     9841 gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc
     9901 agcaaaccag ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg
     9961 cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaatgac gaggacccct
    10021 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg
    10081 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc
    10141 catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca
    10201 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    10261 gaacagcaag cacctgaccg ccac
//
LOCUS       DAZPFP010000132        10049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPFP010000132 DAZPFP010000000
VERSION     DAZPFP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.132"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            67..156
                     /locus_tag="ACHUR4_003030"
                     /pseudo
     CDS             67..156
                     /locus_tag="ACHUR4_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            458..1453
                     /gene="pgl"
                     /locus_tag="ACHUR4_003031"
     CDS             458..1453
                     /gene="pgl"
                     /locus_tag="ACHUR4_003031"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1456521.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1494..2447)
                     /locus_tag="ACHUR4_003032"
                     /pseudo
     CDS             complement(1494..2447)
                     /locus_tag="ACHUR4_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2631..3683
                     /locus_tag="ACHUR4_003033"
     CDS             2631..3683
                     /locus_tag="ACHUR4_003033"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR1456522.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3759..5192
                     /locus_tag="ACHUR4_003034"
     CDS             3759..5192
                     /locus_tag="ACHUR4_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR1456523.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5375..7555
                     /locus_tag="ACHUR4_003035"
     CDS             5375..7555
                     /locus_tag="ACHUR4_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1456524.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7696..8979)
                     /locus_tag="ACHUR4_003036"
     CDS             complement(7696..8979)
                     /locus_tag="ACHUR4_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1456525.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9114..>10049)
                     /locus_tag="ACHUR4_003037"
     CDS             complement(9114..>10049)
                     /locus_tag="ACHUR4_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1456526.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2436 a         2350 c         2546 g         2717 t
ORIGIN      
        1 gaaggagatc acccaattct cccccccgac ttttgacgca gtgtcttgtt aacaggggga
       61 ttgaccatgc ttattggcta tgtacgcgta tcaacaaatg accagaatac cgatctacaa
      121 cgtaatgcgt ttatttgtgc cggaagtgag ttgattgttg cgcgaatggg aagttggcca
      181 gaagttgtta tggcatagtg tttacggtcg ttgatacagg taaacaaaag cggggagagt
      241 catactccct gaatagtcgc gaaaatatct gtggcattgt caaattcgtg tcggtgacta
      301 atattttagt aatatgccat tcgaaatgga gtgctaactt attgatttaa ttgatgtgcg
      361 atattgtatt ttatcgtgtt ttgttgtctg ttttttatcc agtaagtgtt gttatatgta
      421 tgaaagataa gggatttatc atttttgagg tgtgttcatg aagcaaacag tttatatcgc
      481 cagccctgag agccagcaaa ttcacgtctg gaacctgaat catgaaggcg cactgacgct
      541 gacacaggtt gtcgatgtgc cggggcaggt gcagccgatg gtggtcagcc cggacaaacg
      601 ttatctctat gttggtgttc gccctgagtt tcgcgtcctg gcgtatcgta ttgccccgga
      661 cgatggcgca ctgacctttg ccgcagagtc tgtgctgccg ggtagtccga cgcatatttc
      721 caccgatcac caggggcagt ttgtctttgt aggttcttac aatgcgggta acgtgagcgt
      781 aacgcgtctg gaagatggcc tgccagtggg cgtcgtcgat gtagtcgagg ggctggacgg
      841 ttgccattcc gccaatatct caccggacaa ccgtacgctg tgggttccgg cattaaagca
      901 ggatcgcatt tgcctgttta cggtcagcga tgatggtcat ctggtggcgc aggaccctgc
      961 ggaagtgact accgttgaag gggccggccc gcgtcatatg gtattccatc caaacgaaca
     1021 atatgcgtat tgcgtcaatg agttaaacag ctcagtggat gtctgggaac tgaaagatcc
     1081 gcacggcaat atcgaatgtg tccagacgct ggatatgatg ccggaaaact tctccgacac
     1141 ccgttgggcg gctgatattc atatcacccc ggatggtcgc catttatacg cctgcgaccg
     1201 taccgccagt ctgattaccg ttttcagcgt ttcggaagat ggcagcgtgt tgagtaaaga
     1261 aggcttccag ccaacggaaa cccagccgcg cggcttcaat gttgattaca gcggcaagta
     1321 tctgattgcc gctgggcaaa aatctcacca catctcggta tacgaaattg ttggcgagca
     1381 ggggctactg catgaaaaag gccgctatgc ggtcgggcag ggaccaatgt gggtggtggt
     1441 taacgcacac taaccgctga tttaaccggc gcagtctctc ctgcgccggt gtattaactt
     1501 atctcctgta acgcatgtct ctggcgttcg acgatattgg tccacaaatt gtctttatcg
     1561 tcagtccaca aattaatcag caaggaaaaa aagcgttcag ctgccggaga aagcacggca
     1621 tctttgcggc gaataatccc caatgtccga cgtataacgg gttccaccag tgggatacca
     1681 ataatcgaag aatagggcgc atggggcatt gccagaccag gaagcgccga aatccccagt
     1741 cctgcctcca ctagtcctaa tgacgtcgaa agatgacgca cttcgtaaaa ccagtccagc
     1801 ttccagggct tgtcggctag ctgttgttct atcaacaatc ggttgccgct ggaggaacgt
     1861 acgccaatca ttttgtaacc cactaactcc tgccattcta caagctactt tttggccaat
     1921 ggatggtcac gccgacaggc cagcacgaat ggctcgttga ccagtggggt aaaatcaatg
     1981 gatgaatttg tgacgttgtt catgtttatg ccaaagtctg attcgttaca cagcaccgat
     2041 tccatgcaat tattcgtgcc ttgttccaga atccgcactt taatattggg atacagctca
     2101 ttaaatttac cgattgccag cggtaaaaaa taaaatactg cggtcggaat acacgctaat
     2161 gtcaccatac cacgatgata agcattcata tcacgaatat taaaaagcgt ttcatcgaat
     2221 ttctttatta attcccgcgc ctccgggagc aacctttttc ctgcttttgt taacgtaacc
     2281 ttgcgagttg tacgctcaaa tagttgaata tgtaaatctt cctccatctt ttttattctt
     2341 cgcgttaatg caggttgcgt aatattgagt aatttagcgg cattgttaaa tgaactggac
     2401 tctgccagta tgacaaatgc cttcatactt gataattcat gcttcattat tactccggaa
     2461 aatggaagcg acgattttgg ggggctggtc gttaaaaatt ttaactgcat ttagccaact
     2521 taaattaatg aaaaaatgtt attaatcgtt gagctaaagt cattagagat gctttgtcct
     2581 taatgtaacc atatcgcaat aagttatgtt tttaaattga gggcattatt atgaaaaaaa
     2641 taccctgcgt gatgatgcga ggtggaacct cgaggggcgc gttcctgtta gcggaacatt
     2701 tacccgaaga tcaaacgcag cgcgataaaa tattgatggc aattatgggt tccggtaacg
     2761 atctggaaat tgacggtatt ggcggcggta atccactgac cagtaaagtc gccattatta
     2821 gccgttccag cgatccgcgt gctgatgtcg attatctgtt tgctcaagtt atcgtccatg
     2881 agcaacgtgt cgataccacg cctaactgcg gcaatatgct gtctggcgta ggggcattcg
     2941 ccattgaaaa tggtttgatt gcagcgactt cgccagttac ccgtgtacgt atccgcaacg
     3001 tcaatacggg tacgttcatc gaagctgatg tgcaaacgcc aaatggtgtt gtcgagtacg
     3061 agggtagcgc cagaattgac ggcgtaccgg gtactgccgc accggttgcg ctcactttcc
     3121 tgaatgccgc tggaacgaaa actggaaaag ttttcccgac tgataatcag attgattatt
     3181 ttgacgatgt cccggtgacc tgtatcgata tggcgatgcc agtcgtcatt attccggctg
     3241 aatatctggg aaaaacaggt tatgaattac cggcggaact ggatgccgac aaagcattat
     3301 tagcccgcat tgaatctatc cggctacaag cgggtaaagc aatgggctta ggtgatgtca
     3361 gtaatatggt tatccctaaa cctgtgctta tttctccagc gcagaaaggc ggggcaatta
     3421 atgtgcgtta ttttatgccg cattcttgcc atcgcgcgct ggcgataacc ggtgctattg
     3481 ctatttccag tagttgtgca ttggaaggca ccgtcacccg acaaatcgtc ccttctgtag
     3541 gatatggcaa tatcaatatt gaacacccca gtggtgcgct cgacgttcat ttaagtaatg
     3601 aaggtcagga tgccacgacg ttacgcgcat ctgttattcg gacaaccaga aaaatatttt
     3661 ccggtgaagt ttatcttccc tgaaaaaatt cgttgtcagg ataaggacaa tcaataaagg
     3721 acttctgtat gagtcataca gaaagaacag gattttaaat gaataagaaa tcgttatgga
     3781 agctaattct gatattagcg atcccatgta ttattggttt tatgccagct ccggcaggat
     3841 taagcgaact ggcgtgggtg ctttttggta tttacctggc ggccattgtg ggtctggtta
     3901 tcaagccttt cccggaacct gtcgtactgt taattgccgt tgctgcctca atggtggtgg
     3961 tcggtaactt atccgacggt gcgtttaaga ccaccgccgt attaagcggt tactcttcag
     4021 gtactacctg gctggtgttc tcggcgttta ccttaagcgc cgcatttgtg accaccggtt
     4081 taggtaaact tattgcctat ctgctgattg gtaaaatcgg taacaccatg ctgggtctgg
     4141 gttacgttac ggtattcctc gatctggtac tggctccggc aacaccgtct aacaccgcgc
     4201 gtgcgggcgg cattgtgtta ccgatcatca acagcgtggc ggtggctttg gggtccgaac
     4261 cggaaaaaag tccgcgtcgt gtcggacatt acctgatgat gtccatttac atggtcacca
     4321 aaaccaccag ctatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga
     4381 tcaacgacat tctgcacctg caaattagct ggggtggatg ggcgctggcc gccggattgc
     4441 cgggcatcat tatgctgctg gtcaccccgc tggtaattta caccatgtat ccaccagaaa
     4501 ttaaaaaggt ggataacaaa accatcgcta aagcgggcct tgccgaactg ggaccgatga
     4561 aaatccgcga aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc tggattttca
     4621 gtaagtctct gggggttgat gaatccactg tggcaatcgt tgttatggcg accatgctgc
     4681 tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa
     4741 tctggtacgg cggtattatc ggcttaagct ccttattatc gaaagttaaa tttttcgaat
     4801 ggttagctga agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct
     4861 tcgttattat tttcctcagc atcatcgtgc gttatttctt cgcttccggt agtgcctata
     4921 tcgttgctat gttacctgta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt
     4981 taaccgcgct ggcactgttg ttctccaact cctatggcgg catggttact cactatggcg
     5041 gcgcggcagg tccggtcatc tttggcgtgg gttacaacga tattaaatcc tggtggttgg
     5101 tcggtgcggt actgacgata ttaaccttct tggtgcatat caccctcggc gtgtggtggt
     5161 ggaatatgct gatcggctgg aacatgctgt aaatatactc gtcatacttc aagttgcatg
     5221 tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact
     5281 cacttgtcgc cttcctgcaa ctcgaattat ttagagtata tccatttatt atctttctgc
     5341 gcacttcacg gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt
     5401 gtttctcgcc agtaataacg aaataattgc cgaagaacat ttcaccggcg aaattaaaaa
     5461 agaagaagca aaaaaaggca ctattgcctg gtctattctc tcttctcata atacgtccgg
     5521 aaatatggat aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt
     5581 tggtattgta cagaccgcta aagcgtccgg aatggaacgt ttcccgctgc cgtatgtgct
     5641 gaccaactgc cataactcac tctgcgccgt cggcggcacc attaacggtg atgaccatgt
     5701 ttttggttta tcggcagctc agcgttatgg cggtattttt gtgcctccgc atattgcggt
     5761 catccatcaa tatatgcgtg agatgatggc tggcgggggc aaaatgatcc tcgggtcaga
     5821 cagccacacc cgttacggtg cattagggac aatggcagtc ggtgagggtg gcggtgagtt
     5881 ggtaaaacag ctgcttaatg acacctggga tatcgactat ccgggcgtgg ttgcggtgca
     5941 tctgaccgga aaacctgcgc cgtatgtgga gccacaggat gtggcgctgg ctatcattgg
     6001 cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg gaccgggcgt
     6061 tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg atgaccacgg aaaccacctg
     6121 tttaagctcc gtctggcaaa ctgatgaaga agtgcataac tggctggcgc tgcacggtcg
     6181 cggtcaggat tactgccagc ttaaccctca accgatggcg tactacgatg gctgcatcag
     6241 cgttgattta agcgccatca aaccaatgat tgcgctgccg ttccacccga gcaacgtgta
     6301 tgaaatcgac acactgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc
     6361 cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg
     6421 cctgaaagtg cagcagggga ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat
     6481 cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat gacaccttct cgctggcggt
     6541 ttacccgtca tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt
     6601 gattggcgca ggcgcaatca tcagaaccgc attctgtggc ccatgctttg gcgcgggcga
     6661 tacgccaatc aacaacggtt tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga
     6721 aggctctaag ccagccaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat
     6781 cgctgcgact gcggcaaacg gtggctattt aacctctgcc agcgaacttg attgctggga
     6841 caacgtgccg gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt atcagggctt
     6901 tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg aacattaaag actggccgga
     6961 attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc tcgacgaagt
     7021 caccaccacc gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat
     7081 tggtctggcg gggaatgtca gcgagctgac agaggtgttt gcgcgcatta agcagattgc
     7141 tggtcaggag catattgatc cgctgcaaac tgaaattggt agcatggtct atgcggtgaa
     7201 accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga ttggcggtct
     7261 ggcgaatatt gccgaggagt acgcgactaa acgctaccgt tctaacgtca tcaactgggg
     7321 gatgttaccg ctgcagatgg cggaagtgcc aacctttgaa gtgggggatt acatttacat
     7381 ccctggcatt aaagcggcgc tggataatcc gggtacgacg tttaaaggtt atgtgatcca
     7441 tgaagatgcg ccggtaacgg aaattacgct ttacatggaa agcctgacgg ctgaagagcg
     7501 cgagattatc aaggcgggta gtttgattaa cttcaataaa aaccgtcaga tgtaaaaagc
     7561 gccatgtgaa tgtaggtcgc attcggcact tattgtcgga tgcgatgctg gcgcatctta
     7621 tccgacctac aaatcgcatc gaaccgtagg ccggataagg cgtttacgcc gcatccggca
     7681 aatagttaat agctcttact tcttctcctc tgcaaccact ttgctgccca cgccgcggtt
     7741 attgtattcc cacatgcggt tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt
     7801 atcatctacg ctgccggtat tacccgcaaa cggacgatta gagatcaccg catcggccca
     7861 cggtttagcc gtgttaaaac cttcgttgat ggcgctatca cggatcacca cctgaccgtt
     7921 ggtattagca tcaacatcca gcgagcggcc cagttgcgcc acgccatcac cggaagcatt
     7981 gaaacggctg tttacggcga ggaaaccgta atagatgtta gacagcgtag ccggtgcaaa
     8041 cacatatgct tcttgctggg tacgtgagtt caccacgcgg aattcggtgt tatcgaacac
     8101 cactgcgccg cgaccagaaa cgatatccac atccccttca atatagctgt tggtcaccag
     8161 cgtacgcggc tgacggttgg tttccagacg gttctgcaca ccgctgttgg tgacaaagaa
     8221 ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt acttggtcac cgtcagtacg
     8281 cagtgctacc gccggatggt tacctgcatc tacgctatcg cccagcgtgt tttcgatggt
     8341 cagattttgc agttgcagtc cattgttttg tgaccagaag accgcagaac acagaacacc
     8401 gatactgtcg ctgcgtttgc tctggcagct atcgtacata taccacgctg gtttacctgg
     8461 catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg gcagggctca tgcccccatc
     8521 aagggaaagc ccaatcttca catcaatcgg tttttcacct gtaccgtaca gagtgattcc
     8581 acctggagca gcagggacat acaccgttcc ctgatactca ccaggcatca cggcaatata
     8641 ctggcgcttg ttggtgcgct tgataattgc cgcatctacc gccgcctgaa tcgtggtatg
     8701 cgttacacct tgagtacccg ccgggccgac aacaaagtca ggttgcgcag gcagggttat
     8761 cggggacgga ttccacgctg cagcacctgg tgtcagggat gcaaaatagt gttgcgcatc
     8821 gaaattcagc gcttcttttg ccgacagaat cgggcgagaa gaggtaccag gcgcagtttg
     8881 atcagaagga cgttgatcgg gtggtgttga gctacaggcg gtcagcgtca cgccaaaagc
     8941 caatgccagc gccagacggg aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa
     9001 tagaaaaatg aatccgttga agcctgcttt tttatactaa gttggcatta taaaaaagca
     9061 ttgcttatca atttgttgca acgaacaggt cattatcagt caaaataaaa tcattatttg
     9121 atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc catggtgtcc
     9181 gacttgtgcc cgagaagatg ttgagcaaac ttatcgctta tctgcttctc atagagtctt
     9241 gcagacaaac tgcgcaactc gtgaaaggta ggtggatccc cttcgaagga aagacctgat
     9301 gcttttcgtg cgcgcataaa ataccttgat actgtgccgg atgaaagcgg ttcacgacga
     9361 gtagatgcaa ttatggtttc tccgccaaga atctctttgc atttatcaag tgtttccttc
     9421 attgatatcc cgagagcatc aacatgcaat gttgtaggga tggcaatttt tacgcctgtt
     9481 ttgctttgct cgacataaag atatccatct acgatatcag accacttcat ttcgcataaa
     9541 tcaccaactc gttgcccggt aacaacagcc agttccattg caagtctgag ccaacatggt
     9601 gatgattctg ctgcttgata aattttcagg tattcgtcag ccgtaagtct tgatctcctt
     9661 acctctgatt ttgctgcgcg agtggcagcg accgggtttg ttgttatatg gccttcagct
     9721 attgcctctc ggaatgcatc gctcagtgtt gatctgatta acttggctga tgccgccttg
     9781 ccctcgtcta tgtatccatt gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt
     9841 ggagcatcag gcagccccct ccttattgct ttaattttgc tcatgtaatt tatgagtgtc
     9901 ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc gatcaagcca tgaatgtaac
     9961 gtaacagaat tatcactgtt gattctcgct gtcagaggct tgtgtttgtg tcctgaaaat
    10021 aactcaatgt tggcctgtat agcttcagt
//
LOCUS       DAZPFP010000133        10029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPFP010000133 DAZPFP010000000
VERSION     DAZPFP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.133"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            268..1134
                     /gene="folD"
                     /locus_tag="ACHUR4_003038"
     CDS             268..1134
                     /gene="folD"
                     /locus_tag="ACHUR4_003038"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1456527.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1136..1348
                     /gene="ybcJ"
                     /locus_tag="ACHUR4_003039"
     CDS             1136..1348
                     /gene="ybcJ"
                     /locus_tag="ACHUR4_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1456528.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1456..1965
                     /locus_tag="ACHUR4_003040"
     CDS             1456..1965
                     /locus_tag="ACHUR4_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1456529.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2001..3386)
                     /gene="cysS"
                     /locus_tag="ACHUR4_003041"
     CDS             complement(2001..3386)
                     /gene="cysS"
                     /locus_tag="ACHUR4_003041"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1456530.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3560..4054
                     /gene="ppiB"
                     /locus_tag="ACHUR4_003042"
     CDS             3560..4054
                     /gene="ppiB"
                     /locus_tag="ACHUR4_003042"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1456531.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4057..4779
                     /gene="lpxH"
                     /locus_tag="ACHUR4_003043"
     CDS             4057..4779
                     /gene="lpxH"
                     /locus_tag="ACHUR4_003043"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1456532.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4897..5406
                     /gene="purE"
                     /locus_tag="ACHUR4_003044"
     CDS             4897..5406
                     /gene="purE"
                     /locus_tag="ACHUR4_003044"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1456533.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5403..6470
                     /gene="purK"
                     /locus_tag="ACHUR4_003045"
     CDS             5403..6470
                     /gene="purK"
                     /locus_tag="ACHUR4_003045"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1456534.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6607..7500)
                     /locus_tag="ACHUR4_003046"
     CDS             complement(6607..7500)
                     /locus_tag="ACHUR4_003046"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1456535.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7497..8144)
                     /locus_tag="ACHUR4_003047"
                     /pseudo
     CDS             complement(7497..8144)
                     /locus_tag="ACHUR4_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8147..9379
                     /gene="allC"
                     /locus_tag="ACHUR4_003048"
     CDS             8147..9379
                     /gene="allC"
                     /locus_tag="ACHUR4_003048"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR1456536.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9390..>10029
                     /gene="allE"
                     /locus_tag="ACHUR4_003049"
     CDS             9390..>10029
                     /gene="allE"
                     /locus_tag="ACHUR4_003049"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR1456537.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2313 a         2699 c         2668 g         2349 t
ORIGIN      
        1 caggctgaaa attcttttta tattgtcagg tatttcttaa attatcttaa tccttaaaca
       61 aggaaataaa tcagttccag atttacaacg ccatcatgta cgaaaaatga agctttcaat
      121 ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga ggtcgcttca cgcgataaat
      181 ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa tagacgcatc cccttcgacc
      241 tacgtaacag atggaatcct ctctctgatg gcagcaaaga ttattgacgg taaaacgatt
      301 gcgcagcagg tgcgctctga agttgctcaa aaagttcagg ctcgtattgc agccggactg
      361 cgggcaccag gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc
      421 gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct cccgctctta tgacctcccg
      481 gaaaccacca gcgaagcgga gctgctggag cttatcgatg cgctgaatgc cgacaacacc
      541 atcgatggca ttctggttca actgccgtta ccggcgggta ttgataacgt caaagtgctg
      601 gaacgtattc atccggacaa agacgtggac ggtttccatc cttacaacgt cggtcgtctg
      661 tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg gtatcgtcac gctgcttgag
      721 cgttacaaca ttgatacctt cggcctcaac gccgtggtga ttggcgcatc gaatatcgtc
      781 ggtcgcccaa tgagcatgga actgttgctc gcaggttgca ccaccaccgt gactcaccgc
      841 ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc tgttgatcgt tgccgttggc
      901 aagccaggct ttattcccgg tgactggatc aaagaaggcg caattgtgat tgatgtcggc
      961 atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa
     1021 cgcgcctcat acattacgcc tgttcccggc ggcgttggcc cgatgacggt tgccacgctg
     1081 attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc cacagggtga gtaacatggc
     1141 gacattttct ttaggtaaac atccgcacgt tgagctgtgc gacttgctga aactggaagg
     1201 ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga
     1261 cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc ggtcagacag tgagttttgc
     1321 aggacacagc gtacaggttg ttgcctgatc cctttcccgg cctgtatcaa caggccggtt
     1381 ctcttttcag acttttgccc tctacgcccc gttcatccat gctgatagca tcgacatttt
     1441 gccgaagcga ccatcatgcc aaccgttatt acgcacgccg ccgttcccct ttgcattggt
     1501 ttagggttgg gttcgaaagg tctgttattt gccggaatca tcctggcgat gctgcccgat
     1561 gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg tcatcgcggt
     1621 tttacccatt cgctggtgct tgcgtttgtt gtcccgctct tatgtgtgct gattggacgt
     1681 cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc gttgctatcg
     1741 cacagcttac tggattcggt aaccactggc ggtaagggcg ttggctggct gtggccgtgg
     1801 tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt tgcattgtct
     1861 cgttacacca cagcgtacgg gcatcaggtg attatttccg aattgatgtg ggtgtggttg
     1921 ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga tgcgaaaact
     1981 cgcatccggc aattgcgcaa ttacttacga cgccaggtgg tcccttgcgg gccatcttcc
     2041 agcacgatcc ccatctcgtt aagacggtca cgtgccgcat ctgccgccgc ccagtctttc
     2101 gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg
     2161 tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag gcccaataca
     2221 gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc ttctgctttc
     2281 agacggttca cttcacgcgc catatcaaac agcacggaat aggcttccgg ggtgttgaaa
     2341 tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg
     2401 gttttatctg tgccgcgcag cgcagtgtag aggcgctcca gcgccgcacg cgcctgcttc
     2461 aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag gaagtaacgc
     2521 acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg taaagaagtt acccagcgat
     2581 ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta gttcacatac
     2641 tgaccatcat gggcacaggt ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg
     2701 tctgaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt catggccgaa
     2761 cattcaatgt gccagccagg acgacccgcg ccccacggag acggccagct cggttcgccc
     2821 tctttcgaca tcttccacag aacgaagtcc atccggttgc gtttgtcatc aaccacgtca
     2881 acgcgcgcgc cagcctgcag ctggtcgaga tcctgacgcg acagcacgcc ataagttgga
     2941 tcggtcggga cgtcgaacat cacgtcgctg ttgtccgcca cataagcgtg acctttggcg
     3001 atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg cggctccata
     3061 tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat catgcgatcc
     3121 accagcgcca caaagctttc gccattttca ttggcgcgtt tgatgatttt gtcgtcgata
     3181 tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata gcgcgcaacc
     3241 acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta aacggtgatt
     3301 ccacacacgt acatgccgac ttccccggcg tgaataggct taaattcctc tttttggcgt
     3361 gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt gggtaattga
     3421 gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga tgatccgatc
     3481 gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa gcaccaatca
     3541 aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata ttgtcatcaa
     3601 aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact gccgcgaagg
     3661 tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc agggcggcgg
     3721 ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg aagccaacaa
     3781 cggcctgaaa aatacccgtg gtacgctggc aatggcacgt actcaggctc cgcactctgc
     3841 aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct ctggcgaaag
     3901 cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga
     3961 caaaatcaaa ggtgttgcaa ccggtcgtaa cggtatgcac caggacgtgc caaaagaaga
     4021 cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt tattgcagat
     4081 cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt tttagcgggg
     4141 gaagcccgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc atggattggc
     4201 gacgacgatc ccaacccact ccatcgccag atggcggcag cgatcaaagc ggtgtccgat
     4261 tccggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg caaacgcttt
     4321 gcccgtgaaa gcggcatgac attattgccg gaagaaaagg tgctcgaact ttatggtcgc
     4381 cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta tcaggctttt
     4441 cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg
     4501 cgcaaacgca ttgccgcgcg aatgcgcgcg aacagcaaag aagccaacag cagtaaatcg
     4561 ctggcgatca tggacgttaa ccaaaacgct gtggtcagtg cgatggaaaa acatcaggtg
     4621 caatggctaa tccacgggca tacccatcgc ccggcggtgc atgaacttat cgccaaccag
     4681 caacctgctt ttcgcgtggt actgggtgcc tggcatacgg aaggttcgat ggtgaaagtc
     4741 acggctgatg acgttgagct gattcatttt ccgttttaaa aaacccacaa ctttgctgat
     4801 ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg tgccctctaa
     4861 agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa taatccggcg
     4921 cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt cgccgtcgaa
     4981 atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca ccgcaccccc
     5041 gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt gattattgcg
     5101 ggcgcaggcg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct ggtgccggtg
     5161 ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta ctccatcgta
     5221 caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac
     5281 gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca ccagcgtctg
     5341 aatggctggc gcaaagccca gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg
     5401 gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag
     5461 gcgaaccgtt aggcattgct gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc
     5521 cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc gcattaaccc
     5581 gcgagctggc gcgtcatccg gcctttgtga accgcgatgt gttcccgatt attgctgacc
     5641 gtctgactca gaaacagctt ttcgataagc tccacctgcc gaccgcgccg tggcagttac
     5701 ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg gcgattgtta
     5761 agcgtcgcac tggtggctat gacggtcgcg gtcaatggag tttacgcgcc aatgaaaccg
     5821 aacagttacc ggcagagtgt tacggtgaat gtattgtcga gcagggcatt aacttctctg
     5881 gtgaagtatc gctggttggc gcgcgcggct ttgatggcag caccgtgttt tatccgctga
     5941 cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg caggccaacg
     6001 cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag ctgggctatg
     6061 tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc aacgaactgg
     6121 ctccgcgtgt gcataacagc ggtcactgga cacaaaacgg tgccagcatc agccagtttg
     6181 agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg aataatccgt
     6241 cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa ttgccgctgg
     6301 tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg catctgaatt
     6361 tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc ccgctgctgc
     6421 cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa gcaggtgctc
     6481 atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg tacccattgt
     6541 aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg ccggatgctc
     6601 tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt caattcgcga
     6661 taacgcccca atccacgcgg gtttactacg gcttctgaca taaccactca ccgccgttac
     6721 cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg gtgtggcatg
     6781 gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag catcggtgag
     6841 gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga gatctttatc
     6901 aatcaccgct tcactccctg ccccatcttc cgtcacaggc acaccgccgc cgccactgca
     6961 aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga gaattttacg
     7021 cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca tctgccagcc
     7081 gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa taaatttctc
     7141 tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca ccgtcgtaac
     7201 aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa tcatcccctg
     7261 gctttccgcc accagtacat ccagcggata cggttctacc tctttccacg ccagattctg
     7321 taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc gataagaacg
     7381 ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat tttccgccgt
     7441 cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca gtgttttcat
     7501 ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc tgcccctgac
     7561 gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga
     7621 gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata acgtaaataa
     7681 ctgacgctca ccgctgtggt aacgagttga agattgtcgg aacaggcaaa aaacgggcga
     7741 ccggcgcgcg catctaaagc gccataatac caggccgcca gcagcattcc gctcaacgtg
     7801 tcatcatgac ttggcgttaa tccggggcct ttacccagcc agtgccgcca gtcggtctta
     7861 acgccattga gcgcggcctg aaaacagtga cgaaactggc gtaattcagc gggcagcgga
     7921 tcgcttgccg ccatcgccag tggcccgaaa agtccggttt cctccgcgcg ttgcatccat
     7981 gcagctgcaa gcggttgagg atgcgaaggc ggcgtaatac gcagcaaaca ataacgctgt
     8041 ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag ccacaacctg tggtcgttca
     8101 ttgccgcata atccaccgca taacgcatcg aattgcgtgc ggcgaaatta cacattcccg
     8161 tcaagctata gaagaaacgc tgccctggct ttcctctttt ggcgctgacc caacgggtgg
     8221 gatgacccgt ttactttatt cgccggaatg gctggaaacc cagcagcaat ttaaaaaaag
     8281 aatggcagca agtgggctgg aaacacgttt cgatgaagtg gggaatttat acggtcgcct
     8341 gagtggcacc gaatatccac aggaagtggt tctgagcggt tcgcatatcg ataccgtggt
     8401 taacggcggt aacctcgacg ggcaattcgg cgcgctggcg gcgtggctgg caattgactg
     8461 gttgaaaacg caatacggcg caccgttacg tacggtcgaa gtggtggcga tggcagaaga
     8521 agaaggcagc cgcttcccgt atgtcttctg gggcagtaaa aatatctttg ggctggcgaa
     8581 tcctgacgac gtgcggaata tctgtgatgc caaaggaaat agttttgccg atgcgatgaa
     8641 ggcttgcgga tttactctgc cgaacgcccc actaactccg cgtcaggcta taaaagcctt
     8701 tgttgaactg catattgaac agggctgtgt gctggaaagt aatgggcaat caattggcgt
     8761 ggtgaatgca attgtcgggc agcgtcgcta tacggtgacg ctgaacggcg aatcaaacca
     8821 tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt tacgctttca gtcgcatttg
     8881 ccatcagtcg atcgaaaaag cgaaaaagat gggcgatccg ctggttctga cctttggcaa
     8941 agtagagccg cgcccgaata cggtaaatgt ggtgccgggt aaaaccacgt tcaccattga
     9001 ttgtcgtcat accgacgctg ccgtgctgcg cgatttcacc caacagttag aaaacgacat
     9061 gcgggcgatt tgcgatgaaa tggacattgg tattgatatc gatttatgga tggacgaaga
     9121 acccgtgccg atgaataagg agctggtcgc caccctgaca gaattgtgtg aaagagaaaa
     9181 actgaattac cgggtgatgc acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg
     9241 cgtaccaacc tgcatgattt ttatccccag catcaacggg atcagccata acccggcgga
     9301 acgcaccaat attactgacc ttgccgaagg ggtcaaaacg ttggcactca tgctttatca
     9361 acttgcctgg caggaataag gagtcataaa tgggatattt aaataacgtc accggttacc
     9421 gcgaagattt actggctaac cgtgcgattg ttaaacacgg taatttcgca ctgttaaccc
     9481 cggatggtct gataaaaaat attattccgg actttgaaaa ttgtgacgcg acaatcctct
     9541 ccacgccaaa gctgggtgcc tcttttgttg attatctggt cacactgcat caaaacggtg
     9601 gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa
     9661 atatcactgc caaagccgaa ggcaaaacat ttgccttaag cgaaggtggc tatctttatt
     9721 gcccaccagg ctccttaatg acgtttgtta acgcccaggc cgaagacagc caaatctttt
     9781 tatataagcg ccgctatgtt ccggtagaag gccatgcacc gtggctgttt tctggcaatg
     9841 ccagcgaact ggaacgcatt cactatgaag gtatggatga tgtgatcctg ctggattttc
     9901 tgcccaaaga gttaggtttt gatatgaaca tgcatatcct ctcttttgca ccaggtgcca
     9961 gccacggtta tatcgaaaca cacgttcagg aacatggtgc ctatattctt tccggtcagg
    10021 gggtttata
//
LOCUS       DAZPFP010000134         9938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPFP010000134 DAZPFP010000000
VERSION     DAZPFP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.134"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHUR4_003050"
     CDS             complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHUR4_003050"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1456538.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHUR4_003051"
     CDS             complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHUR4_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1456539.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHUR4_003052"
     CDS             complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHUR4_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1456540.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4162..4887)
                     /gene="phoU"
                     /locus_tag="ACHUR4_003053"
     CDS             complement(4162..4887)
                     /gene="phoU"
                     /locus_tag="ACHUR4_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1456541.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4902..5675)
                     /gene="pstB"
                     /locus_tag="ACHUR4_003054"
     CDS             complement(4902..5675)
                     /gene="pstB"
                     /locus_tag="ACHUR4_003054"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1456542.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5767..6657)
                     /gene="pstA"
                     /locus_tag="ACHUR4_003055"
     CDS             complement(5767..6657)
                     /gene="pstA"
                     /locus_tag="ACHUR4_003055"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1456543.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6657..7616)
                     /gene="pstC"
                     /locus_tag="ACHUR4_003056"
     CDS             complement(6657..7616)
                     /gene="pstC"
                     /locus_tag="ACHUR4_003056"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1456544.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7703..8743)
                     /gene="pstS"
                     /locus_tag="ACHUR4_003057"
     CDS             complement(7703..8743)
                     /gene="pstS"
                     /locus_tag="ACHUR4_003057"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1456545.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8991..>9938)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUR4_003058"
     CDS             complement(8991..>9938)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUR4_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1456546.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2564 a         2587 c         2295 g         2492 t
ORIGIN      
        1 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga ttggggatca
       61 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccag
      121 tcatgtaata actaaaagag atgaaatcta cagtatgttt taaatcttct gcgtcacttt
      181 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatatt
      241 ggccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
      301 acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc atattgccga
      361 ttttcgcttc ggggatcagg ctatgatagg ctttaactgc ccgcgcactg gcaaccagtt
      421 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg
      481 tgaatggcgc gtgtaacgac atattgattt cattaaacgt cagccataac gctactttat
      541 gttggtagcg ggtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat
      601 tagcccaacc gccgtagttt ttcaccagcc catatagcat ttcgtaatgg gataacgtta
      661 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc
      721 ccgcttcatt cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa
      781 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtaat
      841 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
      901 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tccagccagg
      961 caccttccac ctgattggca gctgttgcgc caccccaaag aaatgtttct ggaaatgctt
     1021 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggttca cctgcgctta
     1081 tctgcgccgt gccgtggggt aatacgtctg taaaatcatc gctattactg attaataccg
     1141 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
     1201 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
     1261 ttacggtgtc gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa
     1321 tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac
     1381 caaccgaggg cagaatggcg atacctttac ccaacaggcc actggcaaac gtggtatcag
     1441 cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag
     1501 gtggtgtaat aacctctggt gttttctctt gcggggcacc ctgcgctggc tgacgtttac
     1561 tggtgatgaa atgaagcatc aacgtaccaa caaatgcgca acctatggca atgacaccgc
     1621 caataacgct ggcccagacg gtgaaatcaa tccccgttga cgggatggtc tgcatgaagg
     1681 tgaaaatact tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg
     1741 cccccaatgc tccactgata caggcgataa caaaggggta cttacgcggc aggttgacgc
     1801 catataccgc tggttcggtg ataccaaaca gacccgtcaa cgccgctgat cccgccacca
     1861 cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acctgcgcca
     1921 taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga
     1981 tacacagcgg caccaggccc ctgtgcagtc cgaacatgac gaagatttgc cagaagccgc
     2041 ccattaccgc gcccgcaaat gcaggaaccg cctgataaag ccagagataa ccggcggcaa
     2101 tcatttcgct tatccaggtt gatagctgcc ccaccagcag aaaggtgacg ggtgtgataa
     2161 ccatcagaca tagcaatggt gtgaagaaat ttttgattgc cgacggtaac cacgcattaa
     2221 gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg
     2281 agtaattcaa caatgtgacc ggaataccca ggaaatccag ccccggcgca tccgcttttt
     2341 gcccgttctc gaaggcagtc agaattaatg gatgcactaa cgctccacca atcaccatgg
     2401 cagtaaatgg attaccgccg aagcgtttgc ccgcggtgta tcccaggatt atcgggaaga
     2461 acccaaacaa ggcatcactg gcgctgaata aaattaaata agtaccactt tgttcggtcg
     2521 tccactgaaa agtgagcgcc agagccagca tacctttcag gatcccggtt gccgccatca
     2581 gaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt agcagattac
     2641 ctttctcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa
     2701 ccgccaggaa gacatcggcc acatggttac ctatgaccac ctgaaactga ccaccgcttt
     2761 ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat
     2821 cctttaactt aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg ttatctgcgc
     2881 ccccgactcc tgcgactatt tttctggcta actccgtcat aacttgccct ctaccgcttt
     2941 gcggcaaaac tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt
     3001 ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt
     3061 tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta
     3121 cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg
     3181 taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca
     3241 agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttcc
     3301 tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc
     3361 gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca
     3421 ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac
     3481 gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacacg
     3541 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
     3601 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa gacgatcaca ggttgccatc
     3661 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
     3721 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
     3781 ccaattccgc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
     3841 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
     3901 aaaaaaccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa
     3961 caatccagtc atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa
     4021 ttgcgtgcag agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat
     4081 taactttgtg taattttagg aatttataaa gttatatata acaaatccca ataattaagt
     4141 tattgggatt tgtctggtga attatttgtc gctatctttt cccgccagca gtttatccag
     4201 ctcatcaccg cctacatgac ggaaatcctg acccttcacg tagtagaaga taaactcaca
     4261 aatattctgg cagcggtcgc cgatacgttc gattgaacgc gcacagaaca gcgcggtaag
     4321 tacgctcgga atagtgcgcg aatcttccat catgtaggtc atcagttgac gaacaatacc
     4381 ttcgtattcc tgatcgactt ttttatcttc acgataaata cgtaccgctt cgtcaatgtc
     4441 catccgcgcg aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga
     4501 ctccagactt accagcaacg gctgatgctg ctgggagaat ttctccagcg cagtacggca
     4561 gattttgtcc gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat
     4621 aaccagtcgc aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca cgcaggcttc
     4681 atcgatcgcc acttccatca tgttgacgtt cttgtcgcct tcgatcacgc gcttcgccag
     4741 atcgctgtcc tggttatgca tcgcggtgat tgcatcagaa agctgctgct ccaccatgcc
     4801 gcccatggtc atcacctgcg tgcggatact ttccagttcg gcgttgaact ggccggaaat
     4861 atgtttatta agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg
     4921 atgtagtctt ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac
     4981 tcaatcaatt cgcccaggta cataaatgcc gtgtggtcgg aacaacgcgc agcctgctgc
     5041 atgttgtggg tgacgatcac cacggtgtaa tcctgcttca gttcggtgat cagctcttca
     5101 atacgcccgg tagagatggg gtcgagcgcc gaacacggtt cgtcgagcag cagcacttcc
     5161 gggcgaatgg cgataccacg cgcaatacac agacgctgtt gctgaccacc agagagagag
     5221 taaccgctct ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc
     5281 cactgcacgc gctcgtccat gtcggcacgg gagagcttct caaacagacg aacgccaaag
     5341 gcgatgttgt cgtagatgga catcgggaac ggcgtcggtt tctggaacac catgcccact
     5401 ttcgcacgca gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcaagcaga
     5461 atttcacctt ccgcacgctg ctccgggtac agttcaaaca ttttgttgaa ggtacgcagc
     5521 agcgtcgatt taccgcagcc ggacggcccg ataaacgccg taacctggtt tttggcgata
     5581 tccaggttga tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc
     5641 tgaattttac tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtacctg
     5701 atgcttttcg ctaatcaggc ttaccgtagg gccgggtaag gcgttcacgc cgcatccgga
     5761 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
     5821 gacgcacagg gtaatgatca ataccccggc ccaggccagt tgttgccatt ccgcaaacgg
     5881 gctcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
     5941 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg
     6001 ggcaatcgcc agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc
     6061 ggagatcatc ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg
     6121 caccagtttc agcatgttct cagtggtgcg gataacgatc ggcacctgca acagcgccag
     6181 cgcaattacg cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac
     6241 aaacagacca accacaatcg acggcgcgga gagcagaatg tcgttaatga agcgaatcac
     6301 ttctgccagc caggatttac gaccatattc cgccagataa atccccgcca taatgcccag
     6361 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
     6421 cagaccacca ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat
     6481 accgtcgata ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa
     6541 ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg
     6601 cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag
     6661 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
     6721 aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat
     6781 tccgcttccg caaattcgtt cgccagcgca gaggtgatac tattgcccgg catgtacagc
     6841 gaggcgctgt cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
     6901 agcgcgcggc ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga
     6961 acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
     7021 atcaccgggg tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg
     7081 atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc
     7141 gcgccaacaa tcgggatatt cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg
     7201 aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc
     7261 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg
     7321 aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc
     7381 aacgtaccgt agatcggcac cagcgccccg tagatatcgt tcggtgcatc ccactctttg
     7441 gtccatagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
     7501 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg
     7561 aaaattatgt cgcccttttt acccggtggg ttaaaagcgg gcttggttgc agccataagt
     7621 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca
     7681 ccgtacccgg cctggagttt tattagtaca gcggcttacc gctactgtct ttaatattgg
     7741 tcttccacgc agcgcgaacc tgttcaacta cactatccgg caggctggcg taatccaggt
     7801 cgttcgcctg tttagccccg gttttgtacg cccagtcgaa gaatttcagc acctctgtgc
     7861 cttgttctgg tttcttctga tctttgtgga tcagaatgaa cgtggtagag gtaataggcc
     7921 atgcatcttc gcctttctgg ttggtcagat cctgagcgaa ggttttgctc cagtctgcac
     7981 cttttgctgc attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga
     8041 tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc atattcaaca taaccaattg
     8101 cacccggcag acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccga
     8161 tcggccattt tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg
     8221 ccaggtagct ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt actacagcaa
     8281 tgttttgtga aggcagtttc agacccggat tcagtttggc gatggcttca tcatcccact
     8341 tcttgatttt gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag
     8401 acttcagccc tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga
     8461 acagaccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccaaaatcaa
     8521 cggtattagc gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt
     8581 tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg
     8641 ttgcacctgc acctgtcagg cttgcttctg caaacacaga gaaagcactc atcgataagg
     8701 tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga
     8761 ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat
     8821 atataaggaa tatgacattt ttatgacaga aaggaattac caaataactt actgatttta
     8881 aatgtgaaga tgaaaattcc acatgccatc tttttgtcat gatgcattta aaagtgataa
     8941 agaaatgggt gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca
     9001 atctcaacag ttatcgtcgc tgttgcagta aattgccccg gggctgggcg agctccagta
     9061 aggctcgcag gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat
     9121 ttatccatgt ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg
     9181 taaattttta tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt
     9241 tcggtagcat aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac
     9301 tcaatagtga ctgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa
     9361 aaatctgcag ctggtatttc tttaaaatca aaacaaactg gctttaaatt atttatttcg
     9421 caactttgcg gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa
     9481 tccaccgggg tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata
     9541 atcaattttc ctgcaaatgt cttcttgata taaaaatata ctatcgcttt agccccactg
     9601 gatatactta aaggttcaat gcggttgcaa ttgtactttg tattaccaga cgggttgtta
     9661 gcatgatatt caaaaggtgc attcacatac ccaaccccaa gaatatctat agcaatccca
     9721 acatcaaggt tcggattaag atcataataa ttaatattag agtgaacccc agcataagtt
     9781 aatgcctggt ttatttttga cgtataatag atatagggga attctcctac gttaccagta
     9841 tcacaatgac agcgtccaag atatgaggat ccattagtta cagtggtgtt aaatgtcgtt
     9901 ccaaccttat tttcgttcgc ggtaataaga tatcccga
//
LOCUS       DAZPFP010000135         9860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPFP010000135 DAZPFP010000000
VERSION     DAZPFP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.135"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1649
                     /gene="hcp"
                     /locus_tag="ACHUR4_003059"
                     /gene_synonym="priS"
     CDS             <1..1649
                     /gene="hcp"
                     /locus_tag="ACHUR4_003059"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1456547.1"
                     /translation="CVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQGLS
                     AWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLA
                     VDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGA
                     AAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDA
                     GETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAH
                     GYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIV
                     GWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSRE
                     KLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRL
                     VDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNI
                     VTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            1661..2629
                     /gene="hcr"
                     /locus_tag="ACHUR4_003060"
     CDS             1661..2629
                     /gene="hcr"
                     /locus_tag="ACHUR4_003060"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1456548.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2762..4480
                     /gene="poxB"
                     /locus_tag="ACHUR4_003061"
     CDS             2762..4480
                     /gene="poxB"
                     /locus_tag="ACHUR4_003061"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1456549.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4517..5518
                     /gene="ltaE"
                     /locus_tag="ACHUR4_003062"
     CDS             4517..5518
                     /gene="ltaE"
                     /locus_tag="ACHUR4_003062"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1456550.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5529..6959
                     /locus_tag="ACHUR4_003063"
     CDS             5529..6959
                     /locus_tag="ACHUR4_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1456551.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            7058..8071
                     /locus_tag="ACHUR4_003064"
     CDS             7058..8071
                     /locus_tag="ACHUR4_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1456552.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(8068..8898)
                     /gene="amiD"
                     /locus_tag="ACHUR4_003065"
     CDS             complement(8068..8898)
                     /gene="amiD"
                     /locus_tag="ACHUR4_003065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1456553.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(8895..9218)
                     /locus_tag="ACHUR4_003066"
     CDS             complement(8895..9218)
                     /locus_tag="ACHUR4_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1456554.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(9429..>9860)
                     /locus_tag="ACHUR4_003067"
     CDS             complement(9429..>9860)
                     /locus_tag="ACHUR4_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1456555.1"
                     /translation="CLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARD
                     IYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIE
                     ILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2302 a         2632 c         2634 g         2292 t
ORIGIN      
        1 tttgtgtgca atgtgaacaa actatccgta ctccggcaga aaacggctgc tcatacgcgc
       61 aggggatgtg tggtaaaacg gcggaaactt ctgaccttca ggatttactc atcgcggcgc
      121 tgcaagggct ttctgcctgg gcggtaaaag cgcgtgaata cggcatcatc aaccacgatg
      181 ttgacagctt cgcgccacgt gcctttttct caaccctgac caacgttaac ttcgattctc
      241 cgcgtattgt cggctacgct cgtgaagcga ttgccttgcg cgaggcgctg aaagcacaat
      301 gcctggctgt agatgccaac gcgcgcgtcg ataacccgat ggctgacctg caactggtga
      361 gcgatgatct cggcgagctg caacgtcagg cagcagaatt tacccctaac aaagataaag
      421 cggcgattgg cgaaaacatt ctcggcctgc gtctgctgtg cctgtatggc ctgaaaggtg
      481 cggcggccta tatggaacac gcgcatgttc tcggtcaata cgacaacgat atttatgccc
      541 agtaccataa aatcatggcg tggctgggga cctggcctgc cgatatgaac gcgcttcttg
      601 agtgttcaat ggaaatcggc cagatgaact tcaaagtgat gagcattctg gatgcaggcg
      661 aaaccggtaa atacggtcac ccgacgccaa cccaggtcaa cgtcaaagcg acggcgggta
      721 aatgcattct gatttccggt cacgatctca aagatctcta caacctgctg gaacagaccg
      781 aaggcacggg cgttaatgtc tacacccacg gcgaaatgct gcctgcgcat ggctacccgg
      841 agctgcgtaa attcaagcat ctggtcggta actacggcag cggctggcag aatcagcaag
      901 tggagttcgc tcgtttccct ggccccatcg tgatgacctc gaactgcatc atcgacccaa
      961 ccgtaggcgc ttatgacgat cgtatctgga cccgcagcat cgttggctgg cctggcgtgc
     1021 gtcatctgga tggcgaagat ttctctgcgg tcatcgccca ggcgcaacaa atggcgggct
     1081 tcccgtacag cgaaattcct cacctgatca ccgtgggctt tggtcgccag acgctgcttg
     1141 gtgcagccga tacgctgatt gatctggtta gccgtgaaaa actgcgtcat atcttcctgc
     1201 ttggtggctg tgacggcgca cgcggcgagc gccactactt caccgatttc gccaccagcg
     1261 tgccggatga ctgcctgatc ctgaccctcg cctgtggtaa atatcgcttt aacaaacttg
     1321 agtttggcga tatcgaaggt ctgccgcgtc tggtagatgc aggtcaatgt aacgatgctt
     1381 actcagcaat tattctggct gtcactctgg cagagaaact gggctgtggt gtgaacgatc
     1441 tgccgctgtc gctggtgctc tcctggtttg aacagaaagc gattgtcatt ctgctgacgc
     1501 tgctttctct gggcgtgaaa aatatcgtca ccggcccgac tgcacctggt ttcctgacac
     1561 cagacctgct ggcggtgctg aacgagaaat tcggcctgcg ttctatcacc actgttgaag
     1621 aagacatgaa gcaactgttg aacgcgtaag gagtttgtcg atgacgatgc caacgaatca
     1681 atgcccgtgg cggatacagg ttcatcacat tacgcaagaa acgccggatg tgtggacgat
     1741 ttccctgatt tgccacgatc actacccata tcgcgccggg caatatgcac tggtcagcgt
     1801 gcgaaactca gcggaaacgc tgcgtgctta caccatttcc tccacgccag gcgtgagtga
     1861 atatatcacc ctgaccgtgc ggcggattga tgacggtgtc ggctcccagt ggctgacgcg
     1921 cgatgtaaaa cgcggtgatt atctctggct ttcggacgcg atgggggaat ttacctgcga
     1981 cgataaagca gaagataaat tcctgttgct ggcggcaggc tgcggcgtca cgccgattat
     2041 gtcgatgcgt cgctggcttg cgaagaaccg tccacaggcc gatgtgcggg tgatctacaa
     2101 cgtgcgtacg ccgcaggatg ttattttcgc cgatgagtgg cgtaactatc cggtaacgct
     2161 ggtggcagaa aataacgtta ccgaaggctt tatcgctggt cgcctcactc gcgaactgct
     2221 ggcagatgta cctgacttag cttcacgtac cgtgatgacc tgcggcccgg ctccgtatat
     2281 ggattgggta gagcaggaag tgaaagcgct cggcgtgacg cgtttcttta aagagaaatt
     2341 cttcacccca gtagcagaag cggcgaccag cggtctgaaa ttcaccaaac tgcaaccggc
     2401 acgagaattt tacgccccgg ttggcaccac gctactggag gcgctggaaa gcaataacgt
     2461 tccggttgtc gctgcctgcc gtgcgggtgt ttgcggctgc tgtaagacaa aagtggtttc
     2521 cggtgaatat acggtgagca gtacaatgac gctgaccgac gccgaaatcg ctgaaggtta
     2581 cgtactggcc tgctcctgcc atccgcaggg ggatttggtt ctcgcataat cgccttatgc
     2641 ccgatgatat tcctttcatc gggctattta accgttagtg cctcctttct ctcccatccc
     2701 ttccccctcc gtcagatgaa ctaaacttgt taccgttatc acattcagga gatggagaac
     2761 catgaaacaa acggttgcag cttatatcgc caaaacactc gaatcggcag gcgtgaaacg
     2821 catctgggga gtcacgggcg actctctgaa cggtcttagc gacagtctta atcgcatggg
     2881 caccatcgag tggatgtcca cccgccatga agaagtggcg gcgtttgccg ctggcgctga
     2941 agcacaactt tcaggagaac tggcggtctg tgccggatcg tgcggcccag gcaacctgca
     3001 cttgatcaac ggcctattcg attgccaccg caataacgtt ccggtactgg cgattgccgc
     3061 tcatattccc tccagcgaaa ttggcagcgg ctatttccag gaaacccacc cacaagagct
     3121 attccgcgaa tgtagtcact attgcgagct ggtttccagc ccggagcaga tcccacaagt
     3181 gctggcaatt gctatgcgca aagcggtgct taaccgtggc gtttccgttg ttgtattacc
     3241 gggcgacgtg gcgttaaaac ctgcgccaga aggggcaacc acgcactggt atcatgcgcc
     3301 acagccagtc gtgatgccgg aagaagaaga gttacgcaaa ctggcgcaac tgctgcgtta
     3361 ttccagcaat atcgccctga tgtgtggcag cggctgcgcg ggggcgcata aagagttagt
     3421 tgagtttgcc gggaaaatta aagcacctat tgttcatgcc ctacgcggta aagagcatgt
     3481 cgaatacgat aatccgtatg atgttggaat gaccgggtta atcggattct cgtcaggttt
     3541 ccataccatg atgaacgccg acacgttagt gctactcggt actcagttcc cctaccgtgc
     3601 gttctacccg accgatgcca aaattattca gattgatatt aatccagcca gcatcggcgc
     3661 gcatagcaag gtagatatgg cgctggtcgg cgatatcaaa tcaaccctgc gcgcattgct
     3721 gccactggtg gaagaaaaaa ccgatcgcaa gtttctggat aaagcgctgg aagattaccg
     3781 cgatgcccgc aaagggctgg acgatttagc taaaccgagc gagaaagcca ttcacccgca
     3841 atatctggcg cagcaaatta gtcattttgc cgccgatgac gccatcttta cctgtgacgt
     3901 cggcacgcca acggtatggg cggcacgtta tctgaaaatg aacggcaagc gtcgcctgtt
     3961 aggttcgttt aaccacggtt cgatggctaa cgccatgccg caggcgttgg gtgcgcaggc
     4021 aaccgagccg gaacgtcagg tggtcgccat gtgcggcgat ggcggtttta gcatgttgat
     4081 gggcgatttc ctctcagtag tgcagatgaa actgccggtg aaaattatcg tctttaataa
     4141 cagcgtgctg ggctttgtgg cgatggagat gaaggccgga ggttacctga cagacggtac
     4201 tgagctgcac gacaccaact ttgcccgcat tgccgaagcg tgcggcatta cgggtatccg
     4261 tgtagaaaaa gcctctgaag ttgatgaagc cctgcaacgc gccttctcta tcgacggtcc
     4321 ggtgttggtg gatgtggtgg tcgccaaaga agaattagcc attccaccgc agatcaaact
     4381 cgaacaggcc aaaggtttta gcctgtatat gttgcgcgca attatcagcg gacgcggcga
     4441 tgaagtgatc gaactggcga aaacgaactg gctaaggtaa aaagggtggc atttcccgtc
     4501 ataataagga catgccatga ttgatttacg cagtgatacc gttacccgac cgagccgcgc
     4561 catgctcgaa gcgatgatgg ccgccccggt tggggacgac gtttacggag acgaccctac
     4621 cgttaatgct ctgcaggact atgcagcaga gctttccggt aaagaagccg ccatttttct
     4681 gcctaccggc actcaggcca acctggtcgc tctgctcagt cactgcgaac gtggcgaaga
     4741 gtatattgtc ggtcaggccg cgcataacta tctgtttgaa gccggtggcg cagcggtgct
     4801 gggcagtatt cagccgcaac ccatcgacgc ggctgccgac ggcacgctac cgctggataa
     4861 agtggcgatg aaaatcaaac ccgacgatat ccatttcgcc cgcaccaaat tactcagtct
     4921 ggaaaacacc cacaacggca aagtgctgcc gcgtgaatac ctgaaagaag catgggaatt
     4981 tacccgcgag cgcaatctgg cgctgcatgt tgacggtgcg cgcatcttta atgccgtggt
     5041 ggcttacggc tgcgaactga aagagatcac gcaatattgt gattcgttca ccatttgcct
     5101 gtcgaaaggt cttgggacgc cagtcggttc attactcgtc ggtaatcgtg attacattaa
     5161 acgtgccatc cgctggcgga aaatggccgg tggcgggatg cgtcagtccg ggattctggc
     5221 tgccgccggg atgtatgccc tggaaaataa cgtcgcacgg ttgcaggaag atcacgacaa
     5281 cgccgcctgg atggcggagc aactgcgtga agcaggcgcg gatgtgatgc gtcaggacac
     5341 taatatgctg tttgttcgcg tcggggaaga aaatgctgcc gcgttaggcg aatacatgaa
     5401 agcgagaaac gtgctgatta acgcctcgcc gattgtccgc ctggtgacgc atcttgacgt
     5461 ctcgcgcgaa caactggcgg aagtcgccgc ccactggcgc gcattcctgg cgcgttaagg
     5521 agagagacgt gccgcaacgc attttagttc tcggtgccag tggctacatt ggtcagcatc
     5581 tggtgcgcac actcagccag caagggcatc agatcctagc ggcggcacgt catgtcgaca
     5641 ggcttgcaaa gctgcaactg gcaaacgtca gttgccataa agtcgatctc agctggccgg
     5701 ataaccttcc ggccctgttg caggatatcg ataccgtcta ttttctggtg cacagcatgg
     5761 gcgaaggcgg cgattttatc gctcaggagc gccagctggc tctcaacgtc cgcgatgcgc
     5821 tacgtgaagt accagttaag caattaatct ttctcagttc gttgcaggcc ccgccacatg
     5881 agcagtcgga tcatctgcga gctcgtcagg ctacggcgga cattcttcgt gaagcgaatg
     5941 taccagtgac cgaacttcgg gccggaatta tcgttggcgc aggttcagcg gcgttcgaag
     6001 tcatgcgcga tatggtctac aacctgccag tgttaacgcc gccacgctgg gtacgttcac
     6061 gcaccacgcc catcgcgctg gaaaacttgc tgcactatct ggtggcgctg ttagaccatc
     6121 cagccaacga acaccgcatc ttcgaagccg ccggaccaga ggtgctcagt tatcagcaac
     6181 agtttgaaca ttttatggcg gtgagcggta agcgccgctg gttgatcccc atccccctcc
     6241 ccacccgctg gatttcggtg tggtttctca atgtgattac ttccgtaccg cccaccaccg
     6301 ccagggcgtt gattcagggg ctgaaacacg atctgctggc agatgatacc gcgctacgtg
     6361 cactcatccc acaacggctg atcgctttcg atgacgcggt acgtagcacg ttgaaagagg
     6421 aagaaaaact ggtcaactcc agcgactggg gctacgacgc tcaggccttt gcccgctggc
     6481 gaccggagta cggttatttc gccaaacagg cggggtttac cgttaaaacg tccgccagcc
     6541 ttgcggcttt atggctggtg gtgaaccaaa tcggcggtaa agagcgttat ttctttggca
     6601 atattttgtg gcagacacgg gcgttgatgg accgcgcgat cggtcataat ctggcgaaag
     6661 gccgcccgga gcgcgaatat ttacagaccg gcgatgcggt ggatagctgg aaagtgattg
     6721 tcgttgaacc gaaaaagcaa cttacgttgt tatttggcat gaaagcgccg gggctgggac
     6781 gactgtgttt taccctggaa gataaaggtg actatcgtac tatcgatgtc cgcgctttct
     6841 ggcatccgca cggtatgccg gggctgtttt actggttatt gatgatcccc gcgcatctgt
     6901 ttatttttcg cggaatggca aaacaaatcg ccagactggc agaacaaagc acagattaat
     6961 cgacaaaacc tgcgttattc tttcatgttt cccattgcat catgggggaa atcacggaag
     7021 aatgcgcacg tcattttctc aacaacagtg gattgatatg aaggtactgg ttaccggcgc
     7081 caccagcggc ttaggtcgaa acgcggtaga gtttttatgc cagaaaggca tcagcgtgcg
     7141 tgcgagcggt cgcaacgaag caatgggtaa attgctggaa aaaatgggcg cagagtttgt
     7201 tccgacggat ctgaccgagc tggtttcatc acaagctaaa gtgatgctcg cgggcattga
     7261 tacgctgtgg cactgctcca gctttacctc accctggggg acacaacagg ctttcgatct
     7321 ggctaacgtt cgcgccactc gccgcctggg tgaatgggct gtcgcctggg gtgtgcgtaa
     7381 ctttattcat atctcttccc cctccctgta cttcgattat caccaccatc gcaatattaa
     7441 agaagatttt cgccctcacc gcttcgccaa cgagtttgcc cgcagcaaag cagccagcga
     7501 agaagtgatc aatatgcttt cgcaggcgaa tccacaaacg cgctttacta ttctgcgccc
     7561 acaaagtctg ttcggaccgc acgataaagt ctttattccc cgtctggcgc atatgatgca
     7621 ccactacggc agtattctgt taccgcatgg cggcagtgcg ctggtcgata tgacctacta
     7681 tgaaaatgcc gtgcacgcaa tgtggctggc aagccaggaa gcctgcgata agctaccttc
     7741 cgggcgtgtg tacaacatca ccaacggcga gcatcgcaca ctgcgcagca tcgtgcagaa
     7801 gctgatcgac gagttgaata ttgactgtcg tattcgttcc gtcccttacc cgatgctgga
     7861 tatgatcgcc cgcagcatgg agcgtttagg ccgcaagtca gcaaaagagc cgccgctgac
     7921 ccactacggc gcctccaagt tgaattttga ctttacgctg gatattacgc gtgcgcagga
     7981 agagttaggt tatcagccgg tcatcaccct ggatgaaggc atcgagaaaa ctgccgcctg
     8041 gctacgcgac cacggaaaac tgccgcgcta atcctgcccg tatttctcca gcaatgcttc
     8101 ggcaatcgcc tgagtttctg catccgcttc gccgttatat aacgtcgggc ggaaatgcat
     8161 ctggaatgcc ataatcacgc gccgctgctc gcgcggtgtc atatcaggtt ttacgtcata
     8221 accgtagcgc gccaaaagct ccagcaatga cgcggtatct accggtgtgt gcggcgcgcg
     8281 cccggcaagg taaaagttca cccgctgcgc atccggccag gcaccaatcc cctgctgcgc
     8341 cagttgctgc cagggaaata atggccccgg atcgtctttg cgctgcggtg cgatatccgc
     8401 atgtgccact acgttttccg gcttgatgtg ataacgggca ataatatctt tcgccagcgg
     8461 aataagcgcc tgaatctgtg cctgttcaaa cggggcaaaa tatttcactc cggatgattt
     8521 ttgccagcca cggttttcca gctcaatgcc aatagaggtg tcgttaaggc gcgttgcccc
     8581 gcgccaggcg ctaatccccg catgccaggc cagttcttgt tccggcacca gttgccagat
     8641 gcgcggttta ccgttgtatc gcggtggcac cgcagggatc agataatgcg agctgacctg
     8701 cttatcagtc agtgtcgcca gcgagctatc aaaatcatct gcggtgtagt ggatcaccag
     8761 cactttaatg cgcggatacg ccgcctgcgc ctggtgtcgg gtatcaagct gatatccctc
     8821 tttctcgaca atgccttttt cgcctgcaca ccctgccagt aacagagcgg cagcgaccag
     8881 ccagaagact cttctcatcg acgcgttttc accgccgtac cgctgacgct aaccatcagc
     8941 atactgccgt tttgcccgac cgtttcgtag tcgatatcaa taccaactac ggcatcggcc
     9001 cccagcgccc gcgcctggga gcctaattcc tcaaaggcga tctcccgtgc tttacgcagt
     9061 tctttttcat acgcaccgga acgtccgcca acgatatcgc ggataccggc aaagaaatca
     9121 cggaaaatat tggcacctaa aatcgcttcg ccggtcacca caccgcaata ttcaacgatg
     9181 gtcaggcctt ccagagttgg ggttgtagaa aattgcatga tcgatctcct tgtttgttaa
     9241 gccttcgatc tctaaagcat tatcggactg atatgccatg ttgggaagaa ggttttgatc
     9301 agtttaggaa atcatctcat tgtgtcaaaa acacccactt aagtctttac gattatccgt
     9361 tgcataaccc gatattggtt tcattatagg gtaccgctta cctgtcttat tatgttgcac
     9421 caacaacatt aatgagcgga aagaggcaac ttatattgta aatgactcct aaagtcttcc
     9481 ccatcttgtg actgaggtct ataccgctgg attgcttttt ggtcagccgc cgcaaaacaa
     9541 gaaagtaata agatctcaat gcttttgtca ttaatataag cgaagatcat tcttgcaaac
     9601 tcgtatataa acgcccgatc ctgagcgctt aatagtttaa tacaatattt atttttaaga
     9661 gagaaaataa tttcaaggaa agcatccgca gcaaccttca cgttattccc ggcgttcata
     9721 ttatatatat cccttgcaca gatagagtac agagatagta aacagccata caaataatca
     9781 cttattaatt cttttgtcca ttttacaggc aaccagggtt ttatcttgac accatgcata
     9841 ctacattcat gaaaaagaca
//
LOCUS       DAZPFP010000136         9826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPFP010000136 DAZPFP010000000
VERSION     DAZPFP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.136"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHUR4_003068"
     CDS             383..1501
                     /locus_tag="ACHUR4_003068"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1456556.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHUR4_003069"
     CDS             1567..1815
                     /locus_tag="ACHUR4_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR1456557.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHUR4_003070"
     CDS             1880..2248
                     /locus_tag="ACHUR4_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1456558.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUR4_003071"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUR4_003071"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1456559.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUR4_003072"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUR4_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1456560.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUR4_003073"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUR4_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1456561.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUR4_003074"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUR4_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1456562.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUR4_003075"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUR4_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2407 a         2721 c         2378 g         2320 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatac
//
LOCUS       DAZPFP010000137         9825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPFP010000137 DAZPFP010000000
VERSION     DAZPFP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.137"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(78..422)
                     /locus_tag="ACHUR4_003076"
     CDS             complement(78..422)
                     /locus_tag="ACHUR4_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1456563.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            554..2464
                     /locus_tag="ACHUR4_003077"
     CDS             554..2464
                     /locus_tag="ACHUR4_003077"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1456564.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2522..3217
                     /gene="tsaB"
                     /locus_tag="ACHUR4_003078"
     CDS             2522..3217
                     /gene="tsaB"
                     /locus_tag="ACHUR4_003078"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1456565.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3257..3838
                     /gene="yeaY"
                     /locus_tag="ACHUR4_003079"
     CDS             3257..3838
                     /gene="yeaY"
                     /locus_tag="ACHUR4_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1456566.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4044..5729
                     /gene="fadD"
                     /locus_tag="ACHUR4_003080"
     CDS             4044..5729
                     /gene="fadD"
                     /locus_tag="ACHUR4_003080"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1456567.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5811..6926
                     /gene="rnd"
                     /locus_tag="ACHUR4_003081"
     CDS             5811..6926
                     /gene="rnd"
                     /locus_tag="ACHUR4_003081"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1456568.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="ACHUR4_003082"
     CDS             complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="ACHUR4_003082"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1456569.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8001..8618)
                     /gene="yeaW"
                     /locus_tag="ACHUR4_003083"
                     /pseudo
     CDS             complement(8001..8618)
                     /gene="yeaW"
                     /locus_tag="ACHUR4_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(8960..9568)
                     /locus_tag="ACHUR4_003084"
     CDS             complement(8960..9568)
                     /locus_tag="ACHUR4_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1456570.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9738..>9825
                     /locus_tag="ACHUR4_003085"
     CDS             9738..>9825
                     /locus_tag="ACHUR4_003085"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1456571.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2378 a         2412 c         2585 g         2450 t
ORIGIN      
        1 attctctggt cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta
       61 ttttgcttcg ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg
      121 ttcatcaaac ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca
      181 tcccacgctt tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc
      241 agaatgctcg atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg
      301 ttggcggttt gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc
      361 gtgttgttgt ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc
      421 atcattttgt cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt
      481 tgccgtcacc acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc
      541 aacagggggt agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc
      601 aggctttaag ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga
      661 aaaaggccag ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct
      721 ggctcctgcg ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca
      781 ggatcagctc tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa
      841 cgtggcgctg ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc
      901 gctggcgggg ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg
      961 gtctaatcaa acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca
     1021 ggcgtggccg ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta
     1081 taaagattgc tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt
     1141 aaaccatcat ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat
     1201 ccctgaagcg gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca
     1261 gtattttggt cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat
     1321 cgcctaccgc accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc
     1381 ccagagtgcg caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg
     1441 tgagctgtta gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga
     1501 actttgttat gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt
     1561 tgagcgcgcc acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg
     1621 ctacagctac tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag
     1681 cgtggcggat aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc
     1741 agcaacgctg tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca
     1801 ggcggagtcg ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt
     1861 gccgcgcaat ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct
     1921 gcgaccgatc atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat
     1981 gatgcgcgat ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg
     2041 ggaaaccagt aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt
     2101 ggcaaccagt agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat
     2161 tatcgacaaa ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga
     2221 ttgtcgtttg cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac
     2281 tctcaaacag ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat
     2341 ttgtgactat cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc
     2401 cgcgccacgc acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag
     2461 gtaatttgtt acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc
     2521 catgcgaatt ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga
     2581 cggtactgtc aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc
     2641 gatggtgcag gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc
     2701 ttacgggcgc ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg
     2761 gctggcgctt ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca
     2821 aggggcgtgg cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg
     2881 cgaagtttac tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac
     2941 cgaagccgta ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg
     3001 ggtgacggta gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt
     3061 tttgcgcgat ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca
     3121 gatgtttgcc gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa
     3181 caacgtcgca tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa
     3241 aaaaaggagt cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac
     3301 gtttgcgcta atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac
     3361 cacgccgcaa caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga
     3421 ggcacgcttt ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga
     3481 aattgctacc gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg
     3541 tcgcatttat gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt
     3601 tacggtagtc gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa
     3661 atttatggtg atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat
     3721 gccgcctcag ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg
     3781 cggatggggc tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt
     3841 gcttgttttt aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta
     3901 aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg
     3961 tatgatgagt taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga
     4021 acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg
     4081 ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg
     4141 tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca
     4201 agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga
     4261 aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg
     4321 gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc
     4381 ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc
     4441 acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg
     4501 gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc
     4561 gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg
     4621 gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat
     4681 acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg
     4741 cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg
     4801 tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta
     4861 tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa
     4921 aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt
     4981 tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg
     5041 gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc
     5101 tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata
     5161 ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg
     5221 atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc
     5281 aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct
     5341 ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc
     5401 gtaaaaaaga catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg
     5461 tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca
     5521 gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac
     5581 tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc
     5641 gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag
     5701 cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat
     5761 ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca
     5821 cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg
     5881 atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg
     5941 atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag
     6001 cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag
     6061 tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg
     6121 ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg
     6181 ttacgctgga caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc
     6241 agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg
     6301 tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa
     6361 tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc
     6421 agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg
     6481 cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg
     6541 cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa
     6601 tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag
     6661 atgccttacc gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta
     6721 aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc
     6781 tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga
     6841 acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca
     6901 atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc
     6961 cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac
     7021 aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat
     7081 ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata
     7141 aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg
     7201 gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt
     7261 cttcgatagc aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt
     7321 cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt
     7381 cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat
     7441 ggactttctc agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat
     7501 ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg
     7561 acaggaaagg ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa
     7621 ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct
     7681 gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac
     7741 tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat
     7801 cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg
     7861 ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta
     7921 cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga
     7981 aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg
     8041 gcgataccat gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca
     8101 cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga
     8161 tggaacacat cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg
     8221 gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg
     8281 accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa
     8341 ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg
     8401 aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg
     8461 gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca
     8521 atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg
     8581 acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt
     8641 acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg
     8701 ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa
     8761 aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata
     8821 acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc
     8881 acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt
     8941 tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag
     9001 ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg
     9061 ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat
     9121 cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct
     9181 ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga
     9241 tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt
     9301 tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg
     9361 cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc
     9421 tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca
     9481 ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg
     9541 catttttgct ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac
     9601 agaattcttt gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt
     9661 tttctgcgct atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag
     9721 agagtaagga tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg
     9781 ctgctgttcg tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAZPFP010000138         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPFP010000138 DAZPFP010000000
VERSION     DAZPFP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.138"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHUR4_003086"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHUR4_003086"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1456572.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUR4_003087"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUR4_003087"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1456573.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUR4_003088"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUR4_003088"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1456574.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUR4_003089"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUR4_003089"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1456575.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUR4_003090"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUR4_003090"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1456576.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHUR4_003091"
     CDS             complement(6031..6360)
                     /locus_tag="ACHUR4_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1456577.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUR4_003092"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUR4_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1456578.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHUR4_003093"
     CDS             7998..8171
                     /locus_tag="ACHUR4_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1456579.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUR4_003094"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUR4_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1456580.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZPFP010000139         9756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPFP010000139 DAZPFP010000000
VERSION     DAZPFP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.139"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUR4_003095"
     CDS             complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUR4_003095"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1456581.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1480..2820)
                     /locus_tag="ACHUR4_003096"
     CDS             complement(1480..2820)
                     /locus_tag="ACHUR4_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1456582.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUR4_003097"
     CDS             complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUR4_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1456583.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(4608..5057)
                     /locus_tag="ACHUR4_003098"
     CDS             complement(4608..5057)
                     /locus_tag="ACHUR4_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1456584.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUR4_003099"
     CDS             complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUR4_003099"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1456585.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(5857..6186)
                     /locus_tag="ACHUR4_003100"
     CDS             complement(5857..6186)
                     /locus_tag="ACHUR4_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1456586.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUR4_003101"
     CDS             complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUR4_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1456587.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            7421..8269
                     /gene="queF"
                     /locus_tag="ACHUR4_003102"
     CDS             7421..8269
                     /gene="queF"
                     /locus_tag="ACHUR4_003102"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1456588.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUR4_003103"
     CDS             8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUR4_003103"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1456589.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         2434 a         2662 c         2300 g         2360 t
ORIGIN      
        1 caattaaatt caatgaatta aaaaatgatg tagacctgat acatagcaca acaggctatt
       61 ttgcgtttag catcagtctc aaaccggctc cagatagagc cggttttggt tttctgtctt
      121 aacgcaccat gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc
      181 ccatcgcatc gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca
      241 cttgatccat atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc
      301 ctttgatctc aaacggttct ttggtcaggc gctgattacc ttcctgccag atccagtgcg
      361 tatcaatagc agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg
      421 aaatatcgaa gtggttgttt gagtgtgaac cccaggtcag gccaaattca tggcacattt
      481 gcgccacacg taccgaacct tgcatagtcc agaaatgcgg atccgccagc gggatatcaa
      541 cggattgtaa cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg
      601 cagtcggcag gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt
      661 gctccgcacc acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccaattt
      721 taattgcttc gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga
      781 agcgttgcgc cagtgccaca atagactcgg cttcttcttc cccggccagt acaccgcctt
      841 tcagtttgaa atcgttgaag ccatattttt catacgccgc ttccgccagg cgcaccaccg
      901 catccggcgt catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct
      961 ggctttgata cggcagaggt gtggctttgc gattaccgac gaagaacaga taaccgagca
     1021 tttcgacttc gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat
     1081 gctgccccag cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac
     1141 gcaaatcaaa tgtctgcaaa ccgcgcccac tagcatcacg atcggcaaaa gtattacgca
     1201 ccagcgtcag aacgtttttg tattcaccca gcgttttacc taccaccagc ggaatcgcat
     1261 cttccagcgt tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg
     1321 aattatcttt gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat
     1381 tcatcagcat actgtcatga cccgccaccg ggatgacctg catttcagta acaacaggcg
     1441 tcgtaaattg agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg
     1501 acgtttacgg tcaaaggtcc agccggggat caggtactgc atcggacctg cgtcgttacg
     1561 cgcaccgcaa ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag
     1621 ttccacgccc agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg
     1681 atttttggtc aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg
     1741 attacccggt gccgccgcgc ccacatgggt aaatatcgcc agagagatat cgaaatggtt
     1801 gttggaatgg cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc
     1861 accggaaagc gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac
     1921 cgcatgaccc atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt
     1981 cgcccgtcga aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg
     2041 atcttcggca taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag
     2101 cagccatgca ccgttgggat caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc
     2161 acgaacagtg tcgatttctt gctcgccagg taacaccccg cccttaagtt tgaaatcttt
     2221 aaagccatag cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc
     2281 tttctgatgg cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag
     2341 atcggttttg gtccgatcac ctatataaaa cagataaccg aggacggtaa tagtctcgcg
     2401 ttgcttgcct ggccccaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc
     2461 aagcaaggcg gcttccagcg ccgccacggc attaacgcgc aattcaaaag tccaggcacc
     2521 tttgccaaag gtatcaaaat cggctgcctg attaccttta tgtacctgct gaaccacttt
     2581 attcaggcgc gcaacttcct ggcccagaac catcggaata gcatcgacca gtgtttgata
     2641 aatcacctct ccgcccggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac
     2701 cacgatattg cgggtgaagt atgcgttatg cgcaccacca atattgagca acatgctgtc
     2761 atgtccggcg accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgccat
     2821 cttattgccc cgcgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt
     2881 agctcagtac cgcgattagg gcatgaacac caacataaat cagcgcccca ttaaacgagc
     2941 cagtcgtgcc aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga
     3001 acatgttgaa caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta
     3061 ctgcccaacc cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga
     3121 tcatccactc aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca
     3181 ttacgatcgg tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg
     3241 aaataattcc acccagcaca ccgccgataa aaccgcaaac cgccggaacg gaagccacaa
     3301 agcccgcttt cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa
     3361 tgaaaaagta agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc
     3421 gtgacccgag cagctgtttg atctgccccc acttcacgct gaacggcact ttaactttgg
     3481 tgctttgctg atccatattg atcagcgcgc cacccgcggc gatatactcc agctctttct
     3541 gatttacccc cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga
     3601 caatgcccag accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc
     3661 agcccataat cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca
     3721 ccgccgttcc cctttcctgc accggaaacc aggccgcaac aatgcgactg ttgccgggga
     3781 aagatggcgc ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc
     3841 cgaatccact aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga
     3901 tcgaccagaa gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct
     3961 gcccgataac ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa
     4021 ggccgatatc tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt
     4081 tgaaggatgt gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt
     4141 ccacactgct cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta
     4201 gccacgctca ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt
     4261 tgccgttttt ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta
     4321 taaaaagcgc gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg
     4381 cccgattttg gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac
     4441 aagaaaggaa gggtaaaaac gaagaaataa agaagagtat gaaatggatc gcttgactct
     4501 aggcaaacgc cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa
     4561 gattcgattc agcaccgcta aaacgacatt taccgctcgc tgacatatca ggacaacagc
     4621 gtgccccact gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg
     4681 tcaatcaaca gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac
     4741 tgtttgccgc cattgcaaaa atttacataa ctgctgtcgc cgagcgcaat cacgccataa
     4801 cgcagattcg gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta
     4861 tcaggaaggt cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag
     4921 ggcagccagt cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc
     4981 agaattgctt ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa
     5041 ataccaattt ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac
     5101 ccgatggcgc actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt
     5161 gtgataacgc ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag
     5221 taaaaggatg cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat
     5281 gctcagcacc gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat
     5341 gacgcaaatg ggcaagatgt cgtcgaagct gatgtttgcg tccggttttc ggctccagtt
     5401 ccaccaggcc gtagcgcgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg
     5461 ccagaccgcg ataatgcgtc actgctggct gcgggccttt atcttcgcgg gcaaatttat
     5521 cagcgatttt gtccagttct tccaccagtg gataatccaa caccgcttct tccatcaacc
     5581 agccgcgcac aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca
     5641 gcagccgtcc ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca
     5701 gacgatgagc agtaaaaaca tgctgtccta tctggtcacg cacggtttgc atgaccacta
     5761 ctttctcgtc gcgatccagc cagctacggt gaaccagcca gccggagggt ttatttaccg
     5821 caaccagcca ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag
     5881 agcatccagt ttttccaact cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag
     5941 cgccatttca taatagggtg caacagcaaa agcgcccggt aacggctggt tgttatttaa
     6001 caaatcgtgc attcgcggga tcagccccca ctgcaaccac tccagtggtt ccatggtgtc
     6061 cataaagaac ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg
     6121 ctgatgttca cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt
     6181 ggtcataaag caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg
     6241 gagcactgta ttcacagtgc tcccggttcg tttcgcagca ttccagctac ttttgttgct
     6301 ccctgctcat ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta
     6361 ttgcttcctg ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc
     6421 tgacccaccg aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc
     6481 gtcctgcgtc atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt
     6541 gtcctgcaga agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa
     6601 gatagaatcg tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa
     6661 atttcatatg aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat
     6721 tcttatcttt ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat
     6781 ctctcacaac cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc
     6841 cgcaagattt gaggctaaat tcgtccgttt acgtgttccc agcgtctctt tgcacacttc
     6901 accggagaga ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag
     6961 cgtgggcggc agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac
     7021 ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg
     7081 cgcgtgcata tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac
     7141 aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg
     7201 ccagtatacg gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacaattc
     7261 ctcacttaac ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa
     7321 atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat
     7381 acacttgtcg tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca
     7441 tcaggcactt gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc
     7501 cagcctactg caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga
     7561 taacctgcct tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc
     7621 gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct
     7681 gattgagtcg aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa
     7741 ctgggatgag gtgcgccaga cgctggagcg cgacttaagc acttgcgctc agggtaagat
     7801 tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg
     7861 cacttgcatt gatgaccagg atatcactat cgataactat gaattcacta ctgactatct
     7921 ggagaatgcc accagcggtg aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa
     7981 atcaaactgc ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg
     8041 acgccaaatt gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga
     8101 gttccacgaa cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga
     8161 gaaattgagc gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg
     8221 cagtaatagc gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca
     8281 attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa
     8341 tcaaagggaa tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc
     8401 gcttggctcc atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag
     8461 cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt
     8521 gaccgataac agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg
     8581 ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg
     8641 gcgaattatt cgcgctttgc aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt
     8701 ttacgggcaa atccatcata ccgttcgttt tcccaacctg aatctcgaca actccgtcca
     8761 catcactaac ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc
     8821 gccaaatatg gtggtctgct ggggcggtca ctcaattaac gaaaacgagt atttgtatgc
     8881 ccgtcgcgtc gggaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc
     8941 aggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa
     9001 agacagtcgt tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc
     9061 actggtcaac gaattgatca tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg
     9121 tatcgctcac ggcatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta
     9181 tttgctggga attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac
     9241 cggcccgaaa gagagcgccg actacttccg cgtactggac gagtttgtcg tacatacgct
     9301 gggcgaaaac gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg
     9361 tcagatgaaa aaatcgatgc cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta
     9421 cagctttaac tggtcaatgc gcattgcgcc agatttgcaa atgccgtttg agccgtctca
     9481 cgagaatatg gctaatctga agctttaccc ggatcaacct gttgaagtgc tggctgccga
     9541 cctgcgccgt gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc
     9601 cattgaagag tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga
     9661 cgacctgcta cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat
     9721 cccttgctac gaaatctgca cgtaatctcc cctccc
//
LOCUS       DAZPFP010000140         9756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPFP010000140 DAZPFP010000000
VERSION     DAZPFP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.140"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..317
                     /locus_tag="ACHUR4_003104"
     CDS             <1..317
                     /locus_tag="ACHUR4_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1456590.1"
                     /translation="HEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVV
                     ECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMP
                     RV"
     gene            414..566
                     /gene="hokE"
                     /locus_tag="ACHUR4_003105"
     CDS             414..566
                     /gene="hokE"
                     /locus_tag="ACHUR4_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1456591.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            917..1069
                     /gene="hokE"
                     /locus_tag="ACHUR4_003106"
     CDS             917..1069
                     /gene="hokE"
                     /locus_tag="ACHUR4_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1456592.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1191..1811)
                     /gene="entD"
                     /locus_tag="ACHUR4_003107"
     CDS             complement(1191..1811)
                     /gene="entD"
                     /locus_tag="ACHUR4_003107"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1456593.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1986..4226)
                     /gene="fepA"
                     /locus_tag="ACHUR4_003108"
     CDS             complement(1986..4226)
                     /gene="fepA"
                     /locus_tag="ACHUR4_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1456594.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4469..5671
                     /gene="fes"
                     /locus_tag="ACHUR4_003109"
     CDS             4469..5671
                     /gene="fes"
                     /locus_tag="ACHUR4_003109"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1456595.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5674..5892
                     /gene="ybdZ"
                     /locus_tag="ACHUR4_003110"
     CDS             5674..5892
                     /gene="ybdZ"
                     /locus_tag="ACHUR4_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1456596.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5889..9734
                     /gene="entF"
                     /locus_tag="ACHUR4_003111"
     CDS             5889..9734
                     /gene="entF"
                     /locus_tag="ACHUR4_003111"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1456597.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2159 a         2535 c         2674 g         2388 t
ORIGIN      
        1 ctcacgagca tcctgttctg cactctgacc agggatggca gtatcgtatg agaagatatc
       61 aaaatatcct taaagaacat ggtattaaac aaagcatgtc cagaaaaggc aattgtctgg
      121 ataatgctgt ggtggagtgt ttctttggaa ccttaaagtc ggagtgtttt tatcttgatg
      181 agttcagtaa tataagcgaa ctgaaggatg ctgttacgga atatattgaa tactataaca
      241 gcagaagaat tagcctgaaa ttaaaaggtc tgactccaat tgaatatcgg aatcagacct
      301 atatgcctcg tgtataactg tccaactttt tggggtcagt acaccctggg gctgccactc
      361 caaaccagac aatttggatg gtagtccctt cttcgcatgg aggtaatata aacatgctga
      421 cgaaatatgc ccttgtggca gtcatagtgc tgtgtttgac ggtactggga tttacgcttc
      481 tggccggaga ctcgctgtgt gagtttacgg tgaaggaacg taatattgag ttcagggctg
      541 ttctcgctta cgaaccgaag aagtagccgt tgtgcgggga gtaatccccg catatccggt
      601 tgtcaggtca ggatggtaag gcacctgctt tacactttcg cccgcggtca gtgatggctg
      661 cgggcgattt cgttttaaga gcaatgatga gttttgccga cagaagtccg aaacaggact
      721 cactgtataa ataaacagct attttgctaa ggaagggtaa gataacggcg ggtgcctgaa
      781 gctttccggt ttcaggttaa ctctgaggtt tggaaagatg aagccccaag agatatttct
      841 atcaatcctg gggctgccac tccgaacctc aacaactcgg atggtagtcc cttcttcgca
      901 tggaggcaat ataaacatgc tgacgaaata tgcccttgtg gcagtcatag tgctgtgttt
      961 gacggtgctg ggatttacgc ttctggtcgg agactcgctg tgtgagttca ccgtaaagga
     1021 acgtaatatt gagtttaagg ctgttcttgc ttacgaaccg aagaagtagc cgttgtgcgg
     1081 ggagtaatcc ccgcatatcc ggttgtcagg tcagagtggt aaggcacctg ctttacactt
     1141 tcgcccgcag tcagtgatgg ctgcgggcga atcgtaccag atgttgtcaa ttaatcgtgt
     1201 tggcacagag ttatgactat tttttctttt atctgccagt gcacagcaaa catctcattc
     1261 tcacgatgaa tgatgacctg ctgtttattc cagctaatta tctgatagtc cagaaaacct
     1321 gcatcagttt ggatctcact tgccttaaat gcgctctctt tggcggaaaa tgcaagtgtc
     1381 agcgccagag aaaaggctaa accgcagtct gcgagtcgct cgtgttctgc tggtgtaata
     1441 atgttgtctg tcaattcggt cgctgtttgc gcagaaaaaa tttcttcgat atcaacgcca
     1501 atcggttgac gggaaaccac ggccaatgcc gtagccccgc agtggctaat actgccgtat
     1561 acctccgcag gccacaccgg ttggcgtagc tcgccgattg cgggcacaca tttatagcca
     1621 tattcccgca aggcataaac agcagcgatc cgtccggcta aatgctctgt tttacgttta
     1681 cgtccagcgt gttgcagttg tgcgtagtgc ggcagccaga gtaaatcctg ctcacaaaaa
     1741 ttcgccggat cgaactcaac aaaatgcagc gtatgtccgg caaaggggag ggaggtatgc
     1801 gtagttttca tatcgaccat attcgagact gatgacaaac gcaaaactgc ctgatgcgct
     1861 acgcttatca ggcctacgtg atcctgcaat atattgaatt ggcaagatct ttgtaggccg
     1921 gataaggcgt tcacgccgca tccggcgtga acgacgcgca ctttgtcaac aatctgacgt
     1981 tagcatcaga agtgagtatt aatgctcata taccacgtac gtcccggctc gttataggta
     2041 tacgcgccgg caccggcgat atagttggct cctgccagat cgcccgtggt ctgggcatta
     2101 cctgcacgcc acaaacgttt gtcgaacagg ttgtccacgc cgccggtcag actgacattc
     2161 ttcgtcacat cccaggtcgc gctcagaccc acaatgctgt aaggactgat ttctttggtt
     2221 tccgggccaa ccgctggctg acctttatag ttgtacttct tcggctgctg cttgccgtac
     2281 caggtgaagg tcgtttgcat cgacaaatct tcccgtgcct gccagctcag cgttgagttc
     2341 aacgtgtact ccgggatgat cgacaaacgg tcgcccgtgg ttttgttttc actcttcagc
     2401 atataagtga tgttattggt ccacatcacc gtttcgctaa ctggtacgtt taacgatcct
     2461 tccagacctt caaccactgc tttcggcacg ttatcccact gatagagatc agtgccgact
     2521 gcgttttgcc ctacagccac atagcccgct tcaatcttat tgcgataatc gttacggaac
     2581 caggtgacgc ccgccagcca gccgtcgcgt ttgaactcca gaccaatctc tttgttgatg
     2641 ctggtttcta ctttcaggtc atcgttacct tgcagatagc agccgcccgc gctggcatag
     2701 cagccctgac ctttactgta gagaatgtag ttcgggttag tctggtatag gctcggcgct
     2761 ttataggcgc gggcaatgcc tattttcagc gtgaagtcat cgcctaaacc ttgcgatatg
     2821 ttcagcgccg ggctccagtt attgccgaca atactgtgat gatcgaaacg cagccccggc
     2881 gttacgatgg tgctgtcagt cagctccatg ttgttttcgg caaacagcga gaaaatttct
     2941 gcttttgaat acgggctacg gtcggtggca ctcacgccat caatagcgcc accggtattc
     3001 gttccggtca gtgcttgggt gttggaactt aagtccttca tccgttgctg attccactcc
     3061 gtacccagcg tcagcgtctg gttaacgagg aaatcaatcg gcaggttaac ttcgctgtgc
     3121 agcatcacgt catcgagatc gatatcgacg aaatcctgtg tcgctttttc gttaaatttc
     3181 ccttcggtac cgcccgccag accttccgga atacgcgagt tacgggtgtg ttcatactgc
     3241 acccagttgc tggtggtcac gccgttatcc cagccaccgt tccaggtcag cgcgtagttc
     3301 tggcgataca ggcggttggt ttcatcgcca tatttcgagc gagtgtaagc gtcagagttg
     3361 gtgttctggg tatcgcccgc atacaggtta ccctggcggc tgtaacccgc ttccagttcc
     3421 agcgattgca atggtgcgaa gtcccagcgc accacgccgt taatatcttt attgatcacc
     3481 ccttcgcgcc cggctggtaa cgtcgtggca tacgttccgg cacgcgcgga ctgatggccc
     3541 tggttgatat cccacgcgtc agcctgagtt ttgtcgaggt tgccatacaa gcggaagctg
     3601 aattcgtcgc ccagcggacc ggtcaggcta aagttagtgc gtttggtggc accttcctct
     3661 ttatgttctg gcgcattgaa atatgcgtcc caggagccgt gccactcgcc gctgcctttt
     3721 ttggtaatga tgttaaccac gccgcccgcc gcgccgttgc cataacgcgc agctgccgga
     3781 ccacgcagaa cttcaatacg ttcaatcatt tcaggtggca cccaggaggt atcaccacgg
     3841 gtatcgcgct cgccacgcca gccctgacgc accgagttac ggctgcttac cggcttgccg
     3901 tcaatcaaaa tcagcgtgtt ttccggaccc ataccgcgaa tatcaatctg tcggttattc
     3961 ccacgctgac cactggtgga gttaccggtc aggttaacgc ctggcatggt acggatgatc
     4021 tccgacacat cgcgggcaac cgggtttttg cggatttcat ctgcggtgat ggtcgaaacg
     4081 ccaggagcct gcaagttctg ctcggcggcg gtaacgacaa tggtatcgtc atgtgaaaca
     4141 ggtgtatcgg tcggctcttg tgcctgcgct accccataaa tccccagatt gaccaacaag
     4201 gccagggaat gaatcttctt gttcattgtt ttattcctgc atttttgcca cgaattgcaa
     4261 ctgtcgggca tggtcgtcat caacacgacg catcccgtta ccgcgaaaac ctttgatcct
     4321 gaaagacacg cagtgcagtt ggttaattaa tgtccgcgct tcccacggcg cgccattacg
     4381 ctattgcaaa tgcaaatagt tattaataac attatcaata tatttctgca atcaatgaaa
     4441 aattgcacag taaacatggg gttatggtgt gacggcgtta aaagtaggaa gtgagagctg
     4501 gtggcagtcg aaacatggcc cggaatggca gcgtctgaat gacgaaatgt ttgaggtcac
     4561 tttctggtgg cgtgatcccc aaggttctga agaatactcg acgataaagc gcgtatgggt
     4621 ctacatcact ggtgtgaccg atcatcatca gaacagccag ccccagtcga tgcagcgaat
     4681 tgcaggtact gatgtctggc agtggacgac acaactcaat gccaactggc gcggcagcta
     4741 ctgctttatt cccaccgaac gcgatgacat tttttctgca ccatcccccg atcgcctcga
     4801 attacgtgaa ggctggcgaa aactattacc ccaggcaata gccgatccgc tgaatccaca
     4861 aagctggaaa ggcgggctag ggcacgctgt ttctgcactc gaaatgccgc aagcgcctct
     4921 gcaaccggga tgggattgtc cgcaagcgcc agaaatacca gccaaagaaa ttatctggaa
     4981 aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt accaccggcg atgtaacagc
     5041 agaagaacgc ccgctggcag ttttgctcga tggcgaattt tgggcgcaaa gtatgcccgt
     5101 ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt cctcccgccg tgtatgtgtt
     5161 gatcgacgct atcgacacca cgcaccgcgc ccacgaactg ccgtgtaatg cggatttctg
     5221 gctggcagta cagcaagagt tattacccct ggtgaaagtt attgcccctt ttagcgatcg
     5281 cgccgatcgc accgtggtcg ccgggcagag ttttggtggg ctgtccgcgc tgtatgccgg
     5341 actgcactgg cctgaacgct ttggctgtgt attaagccag tcaggatcgt actggtggcc
     5401 gcatcggggc gggcagcaag agggcgtgtt acttgaaaag ctaaaagctg gtgaagttag
     5461 cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc gagccgatga tcatgcgggc
     5521 caatcaggcg ctgtatgcgc aattacaccc tataaaagaa tccattttct ggcgtcaggt
     5581 tgacggcgga catgatgcgc tttgttggcg cggtggcttg atgcaggggt taatcgacct
     5641 ctggcaacca cttttccatg acaggagttg aatatggcat tcagtaatcc cttcgatgat
     5701 ccacagggag cgttttacat tttgcgcaat gcgcaggggc aattcagtct gtggccgcaa
     5761 caatgcgcct taccggcagg ctgggacatt gtgtgtcagc cgcagtcaca agagtcctgc
     5821 cagcagtggc ttgaagccca ctggcgtact ctgacaccgg cgaatttcac ccagttgcag
     5881 gaggcacaat gagccagcat ttacctttgg tcgccgcaca gcccggcatc tggatggcag
     5941 aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca ttacgttgag ttaaccggag
     6001 aggttgatgc gccattactg gcccgcgcgg tggttgccgg attagcgcaa gcagatactt
     6061 tgcggatgcg ttttacggaa gataacggcg aagtctggca atgggtcgat gatgcgctga
     6121 tattcgaact gccagaaatt atcgacctgc gaaccaatat tgatccgcac ggtactgcgc
     6181 aggcattaat gctggcggat ttgcaacaag atttgcgcgt cgatagcggt aaaccactgg
     6241 tctttcatca actgatacag gtggcagata accgctggta ctggtatcag cgttatcacc
     6301 atttactggt cgatggcttc agttttccgg ccattacccg ccagatcgcc aacatttact
     6361 gcgcattgct gcgtggcgaa ccaacgcctg cttcgccgtt tacgcctttc gctgatgtag
     6421 tggaagagta ccagcaatac cgcgaaagcg aagcctggca gcgtgatgcg gcattctggg
     6481 tggaacagcg tcgccaactg ccaccgcccg cgtcactttc tccggcacct ttagcggggc
     6541 gcagcgcctc ggcagatatt ttgcgtctga aaatggaatt taccgacggg gaattccgcc
     6601 agctggctac gcaactttca ggtgtgcagc gtaccgattt agcccttgcg ctgacggctt
     6661 tgtggctggg gcgattgtgc aatcgcatgg actacgccgc cggatttatc tttatgcgtc
     6721 gactgggctc ggcggcgctg acggctaccg gacccgtgct caacgttttg ccgctgggta
     6781 ttcatattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggca acccgactgg
     6841 cagcacaact gaaaaaaatg cgtcgtcatc aacgttacga tgccgaacaa attgtccgtg
     6901 acagcgggcg agcggcaggt gatgaaccgc tgtttggtcc ggtactcaat attaaggtat
     6961 ttgattacca actggatatt cctggtgttc aggcgcaaac ccataccctg gcaaccggtc
     7021 cggttaatga ccttgaactg gccctgttcc cggatgaaca cggtgatttg agtattgaga
     7081 tcctcgccaa taaacagcgt tacgatgagc caacgttaat ccagcatgct gaacgcctga
     7141 aaatgctgat tgcccagttc gccgcggatc cggcgctgtt gtgcggcgat gtcgatatta
     7201 tgctgccagg tgagtatgcg cagctggcgc agatcaacgc cactcaggtt gagattccag
     7261 aaaccacgct gagcgcgctg gtggcagaac aagcggcaaa aacaccggat gctccggcgc
     7321 tggcagatgc gcgttacctg ttcagctatc gggaaatgca cgagcaggtg gtggcgctgg
     7381 cgaatctgct gcgtgagcgc ggcgttaaac caggggacag cgtggcggtg gcactaccgc
     7441 gctcggtctt tttgaccctg gcactccatg caatagttga agctggcgcg gcctggctac
     7501 cgctggatac cggctatccg gacgatcgcc tgaaaatgat gctggaagat gcgcgtccgt
     7561 cgctgttaat taccaccgac gatcaactgc cgcgctttag cgatgttccc aatttaacca
     7621 acctttgcta taacgccccg cttacccccc agggcagtgc gccgctgcaa ctttcacaac
     7681 cgcaccacac ggcttatatc atctttacct ctggctccac cggcaggccg aaaggggtaa
     7741 tggtcgggca gacggctatc gtcaaccgcc tgttgtggat tcaaaatcat tatccgctca
     7801 caggcgaaga tgtcgttgcc caaaaaacgc cgtgcagttt tgatgtctcg gtgtgggagt
     7861 ttttctggcc gtttattgcc ggggctaaac tggtgatggc tgaaccggaa gcgcaccgcg
     7921 acccgctcgc tatgcagcaa ttctttgccg aatatggcgt aacgaccacg cactttgtgc
     7981 cgtcgatgct ggcagcattt gttgcctcgc tgactccgca aaccgctcgc cagagttgcg
     8041 tgacgttgaa acaggttttc tgtagtggtg aggccttacc ggctgattta tgccgcgaat
     8101 ggcaacagtt aaccggcgcg ccgttgcata acctgtatgg cccgacggaa gcggcggtag
     8161 atgtcagctg gtatccggct tttggcgagg aactggcaca ggtgcgcggc agcagtgtgc
     8221 cgattggtta tccggtatgg aatacgggtc tgcgtattct tgatgcgatg atgcatccgg
     8281 tgccgccggg tgtggcgggt gatctctatc tcaccggtat tcaactggcg caggggtatc
     8341 ttggacgacc cgatctgacc gccagccgct ttattgccga tccttttgct cctggtgaac
     8401 ggatgtatcg taccggagac gttgcccgct ggctggataa cggcgcggtg gagtacctcg
     8461 ggcgcagtga cgatcagcta aaaattcgcg ggcagcgtat cgaactgggc gaaatcgatc
     8521 gcgtgatgca ggcgctgccg gatgtcaaac aagccgttac ccacgcctgt gtgattaacc
     8581 aggcggcagc cactggtggt gatgcgcgtc agttggtggg ctatctggta tcgcaatcgg
     8641 gcctgccgtt ggataccagc gcattgcagg cgcagcttcg cgaaacattg ccgccgcata
     8701 tggtgccagt cgttctgctg caactgccgc agttgccact tagcgccaac ggcaagctgg
     8761 atcgcaaagc cttaccgttg cctgaactga aggcacaagc gccagggcgt gcgccgaaag
     8821 cgggcagtga aacgattatc gccgcggcat tcgcgtcgtt gctgggttgt gacgtgcagg
     8881 atgccgatgc tgatttcttt gcgctgggcg gtcattcgct actggcaatg aaactggcag
     8941 cgcagttaag tcgacagttt gctcgtcagg tgacgccggg gcaggtgatg gtcgcgtcaa
     9001 ccgtcgccaa actggcaacg attattgatg gtgaagagga cagctcccgg cgcatgggat
     9061 tcgaaaccat tctgccgttg cgtgaaggta atggcccgac gctgttttgt ttccatccgg
     9121 cgtccggttt tgcctggcag tttagcgtgc tctcgcgtta tctcgatcca caatggtcga
     9181 ttattggcat tcagtcgccg cgccctcatg gacccatgca gacagcgaca aacctggatg
     9241 aagtctgcga agcgcatctg gcaacgctac ttgagcaaca accgcacggc attgcggcgc
     9301 ggctgcgtgc ccgtggcgaa caggtggcat ttcttggttt gctggatacc tggccgccag
     9361 agacgcaaaa ctggcaggaa aaagaggcta atggtctgga cccggaagtg ctggcggaga
     9421 ttaaccgcga acgcgaggcc ttcctggcgg cacagcaggg aagtacttca acggagttgt
     9481 ttaccaccat tgaaggcaac tacgctgatg ctgtgcgcct gctgacgact gctcatagcg
     9541 taccgtttga tggtaaagcg acgctgtttg ttgctgaacg cacgcttcag gaaggtatga
     9601 gccccgaacg cgcctggtcg ccgtggattg cggagctgga tatctatcgt caggattgtg
     9661 cgcatgtgga tattatctct ccaggggcgt ttgtaaaaat tgggccgatt attcgcgcaa
     9721 cgctaaacag gtaaattaat attatctata aaccca
//
LOCUS       DAZPFP010000141         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPFP010000141 DAZPFP010000000
VERSION     DAZPFP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.142"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHUR4_003112"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHUR4_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUR4_003113"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUR4_003113"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1456598.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUR4_003114"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUR4_003114"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1456599.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUR4_003115"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUR4_003115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1456600.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUR4_003116"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUR4_003116"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1456601.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUR4_003117"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUR4_003117"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1456602.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHUR4_003118"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHUR4_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHUR4_003119"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHUR4_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1456603.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUR4_003120"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUR4_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1456604.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUR4_003121"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUR4_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1456605.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUR4_003122"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUR4_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1456606.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUR4_003123"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUR4_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1456607.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUR4_003124"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUR4_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1456608.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZPFP010000142         9649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPFP010000142 DAZPFP010000000
VERSION     DAZPFP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.143"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            110..724
                     /gene="evgA"
                     /locus_tag="ACHUR4_003125"
     CDS             110..724
                     /gene="evgA"
                     /locus_tag="ACHUR4_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1456609.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            729..4322
                     /gene="evgS"
                     /locus_tag="ACHUR4_003126"
     CDS             729..4322
                     /gene="evgS"
                     /locus_tag="ACHUR4_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1456610.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4378..5523)
                     /gene="yfdE"
                     /locus_tag="ACHUR4_003127"
     CDS             complement(4378..5523)
                     /gene="yfdE"
                     /locus_tag="ACHUR4_003127"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1456611.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5597..6541)
                     /gene="yfdV"
                     /locus_tag="ACHUR4_003128"
     CDS             complement(5597..6541)
                     /gene="yfdV"
                     /locus_tag="ACHUR4_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1456612.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6611..8305)
                     /gene="oxc"
                     /locus_tag="ACHUR4_003129"
     CDS             complement(6611..8305)
                     /gene="oxc"
                     /locus_tag="ACHUR4_003129"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1456613.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8359..9609)
                     /gene="frc"
                     /locus_tag="ACHUR4_003130"
     CDS             complement(8359..9609)
                     /gene="frc"
                     /locus_tag="ACHUR4_003130"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1456614.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2861 a         2191 c         1990 g         2607 t
ORIGIN      
        1 cttaaaggaa actcagattt tcttattttt attgagaaaa tgagatgacg ccttatgtct
       61 gtattactac agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat
      121 aattattgat gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga
      181 tattgaaatc ttagcggagt taactgaagg cggaagcgcc gttcagcggg tggaaacact
      241 taagcctgat atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt
      301 agaaacgctg aggaagcgcc aatatagcgg aattattatt atcgtctccg ctaaaaatga
      361 ccatttttac gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga
      421 aggcatgaac aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc
      481 cttctctctc aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt
      541 atcgaaacaa gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat
      601 tgcggaaaaa atgttcatca gcaacaaaac tgtcagcacg tataaaagtc gcctgatgga
      661 aaaattagaa tgtaaatcac tgatggatct ttacacattc gcgcaacgta acaaaatcgg
      721 ctaatcacat gaagttttta ccctattttt ttcttctctg ttgtggtctt tggtcgacca
      781 taagtttcgc agacgaagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca
      841 cacttgatcc agtacgtctg agcaacaagg aattacgttg gttagcgagc aaaaaaaatc
      901 ttgtgattgc agtacacaag tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac
      961 aggttcgtgg tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat
     1021 taacactccg ggaatacgca gatcatcaaa aagcaatgga cgcgcttgca gaaggtgaag
     1081 tcgatatagt gttatcacat ttagttactt cgccgcctct taataatgac attgctgcaa
     1141 ccaaaccatt gataattacc tttccggcgc tggtaaccac ccttcacgac tcaatgcgac
     1201 cgcttacctc accaaaacca gtaaatattg ctcgggtagc aaattacccc ccagacgagg
     1261 taattcatca atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat
     1321 tagcatccgt ctcagctggg cacaatgatt actttattgg tagtaacatc attaccagca
     1381 gtatgatttc ccgctatttc actcactcct taaatgtagt gaaatattat aactcgccgc
     1441 gtcaatataa ttttttcttg accagaaaag aatctgtcat tcttaatgaa gtactcaata
     1501 gatttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata
     1561 caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga
     1621 ttaagcagca tcccaattta aaggtgctgg aaaatcctta ctcgcccccc tattctatga
     1681 cggatgaaaa tggctcggtt cggggcgtta tgggggacat tcttaatatt attaccttgc
     1741 aaacaggttt aaatttttct ccgatcaccg tttcacacaa tatccatgct ggaacacaac
     1801 ttagccccgg aggatgggat ataatacctg gcgctattta tagtgaagat cgagaaaata
     1861 atgttttatt tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc
     1921 ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc
     1981 tgcattcgca attaaaagag atgtatccgg aggttgaatg gatacaggtc gataatgcca
     2041 gcgctgcatt tcacaagatt aaggaaggtg aacttgatgc tctggtcgcg acacagctaa
     2101 attcgcgtta catgatcgat cattactatc ctaatgaact ttatcatttt cttattcctg
     2161 gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata
     2221 ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg acggaaaaat
     2281 ggattaaaat gcccaatgtg accattgaca catgggacct atatagcgag caattttata
     2341 ttgttacgac attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt
     2401 tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa atatcattcc
     2461 gaaaagcact ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg
     2521 tcattagtca taatagtgct tttgaacatt atttcactgc ggattactac aaaaatgcaa
     2581 tgttaccatt agaaaacagt gattcaccct ttaaagatgt tttttctaat gcgcatgaag
     2641 tcacagcaga aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa attgataatg
     2701 gcatcgagaa aagatgcatt aatcactggc atacattatg caatcttcct gcaagtgaca
     2761 atgcagtata tatttgtggt tggcaagata ttactgaaac gcgtgatcta attaatgcac
     2821 tcgaggtaga aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag tttctggcaa
     2881 cgatgagtca cgaaataaga acaccaataa gctctattat gggcttcctg gaacttctgt
     2941 cgggttctgg tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg
     3001 gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta
     3061 actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact
     3121 ctttcggtgc gattgctgca agcaaatcga tcgcattaag ttgcagcagt acatttcctg
     3181 atcattacct ggttaagatc gaccctcagg cgtttaagca ggtcttatca aatttactga
     3241 gtaatgctct caaatttacc accgaggggg cagtaaaaat tacgacctcc ctgggtcaca
     3301 ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc
     3361 aggaagaaca acaacaactg tttaagcgct acagccaaac aagtgcaggt cgtcagcaaa
     3421 caggttctgg tttaggctta atgatctgca aagaattaat taaaaatatg cagggcgatt
     3481 tatcattaga aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa
     3541 ttagccagca agtggcgact gtcgaggcaa aagcagaaca acccatcaca ctacctgaaa
     3601 agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc
     3661 taaatctatt aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag
     3721 tcagtatgca acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt
     3781 ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag
     3841 ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt
     3901 tcaaaccgtt gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc
     3961 atattgcacc tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaatg
     4021 atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac
     4081 ccgctgcgtt tcatgcacta gaagctggcg ataacagaac tttccatcag tgtattcatc
     4141 gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt
     4201 tagaaataac acctgcttca gatgacagta agcctgaaat tcttcagctg ctgaactctg
     4261 ttaaagaaca cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact
     4321 aaattgcggc tcccacaatg ttctaatgtg ggagctattt accagcacat cttacattta
     4381 tgatgagaac tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat
     4441 aacatgcggg tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattcctcc
     4501 ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct cagccacact
     4561 taataacggc gcgacgggca caccaacttc atgtattttg gctaaccaaa cttcagctgc
     4621 ctgcgttttt aacgttcgct caatatattg tttaagaata gcctggtttt gtacgcgtaa
     4681 aatattgctg ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa
     4741 cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtatcgaa
     4801 aacatcaaaa ggtgccatgt agggatggcg atttcccagg cgttgtggtg acttccctgt
     4861 cgcaatatat gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat
     4921 atcgacatgc gcccctctct ggctcttttc gcggccataa agtgcactca ctattccgct
     4981 gaataaatag acaccgccgc atagatccgc aagagaggta ccaacacgca ctggcggagc
     5041 atcagggtac cctgtttcca tcattatccc actcattgcc tgaatgatgg tatcgtaggc
     5101 aggagcatct tttagcggac cggtatgtcc gaaacctgac gatgaagcat atatgaggcg
     5161 cgggttgatt tcttgtaacc tttcccatga aaaccccagt ttttccattg tacctaggcg
     5221 aaaattctca gctaatacat cagcttgttt gagcatattt ataaatatac ttttatcgtg
     5281 atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag
     5341 tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac ctggtggctc
     5401 aactttaatt acccttgcgc ccatattata aagaagttga gttccgaaag gtccattaag
     5461 gacatgtgtc atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt
     5521 catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa
     5581 tcaataacat gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga
     5641 cgacaaagcc cagtacgctc acagccaatg acgctgtacc agtacgggtg tagacattaa
     5701 accggctggc aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca
     5761 tctgcaaatg ttcgctgttc aaatgacatg ccataccaac gagaagcagc gccagtggca
     5821 tcagaatcaa cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg
     5881 cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat
     5941 taaaggttgg gtcccatgct gccggaattt ttaccccaac cagcaccagg atcgttgcca
     6001 gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat
     6061 tcttcttacc atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa
     6121 ttgcgttaac aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat
     6181 aaataggatc gagaactgca aaccccagga atccaatggt aggtgaaccc gcaattaatg
     6241 cacatacagc tgcttctgca tgggtacgtt taaaaaattt gtagcaacca aaccaggaga
     6301 aaaagaaaca tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa
     6361 tcatttccct gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga gcgtagttta
     6421 ataccagttt attaaatgcg cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa
     6481 aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca
     6541 taatagtatt tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg
     6601 atgtcagtat ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt
     6661 cagttcctgc tgcagggtcg ataaccacat taataatggt cggtttgcgc gactgaatac
     6721 cggtggttaa agcatgacga agttcatctg tcgtggtgac gttatagcca acgccacgaa
     6781 acgcatccat taatttgtca taccttgcat ggtgcaacag atccgttggt gatggtgcac
     6841 cagcgccact gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga
     6901 tcgtcaccgg caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag
     6961 cactatcacc ttcaatggcg acaaccggag aaccagaagt cacgctagca ccgatggcat
     7021 agcccatacc gatgcccatg acaccccagg taccacaatc cagacgacga cgtggtttat
     7081 acatatcaat aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa
     7141 tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg
     7201 gttgagtatc tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat
     7261 ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca
     7321 taccttgcat actggatgca atatcaccaa cgactggcac agcaatgggg cggttgctgt
     7381 caatttcctg cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt
     7441 taccgtgtgc caataaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg
     7501 ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg cgtatcttca aggatccctt
     7561 tcgccataga cattggcagg aatggaatct gagcactttc aataaattca cgaagctgtt
     7621 catcagcttg tgaatacgcc gcgcctttgc caaggataat taatggccgt tcagcttttg
     7681 ctaaaagcga aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgacggat
     7741 tttcaacttt aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat
     7801 ttgctggcaa atcaagataa actccgccag ggcgacccga tacagagacc cggatagcgc
     7861 gtgccaatgc aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg
     7921 gttttgccgc attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg
     7981 cgcggtcgct ggagccgcta atcatgataa tcggaaaacc atttaccgtt gcgttggcca
     8041 atgcggtcaa accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt
     8101 gggtaagaaa accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa
     8161 tataacgaat gccttccgcc tgggcatggc gtgccatatc cgtcacagga atacctacaa
     8221 caccataaat agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat
     8281 ctgtcatttg aagttgatct gacatgatta ataacctcga aatttttata aattgggagc
     8341 cagaagcccc cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc
     8401 gctataaccc agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt
     8461 aatatccggc gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg
     8521 caacggttgt tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat
     8581 ttctttcata cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc
     8641 ttcatgttta tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg
     8701 tggctgtcgg gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat
     8761 ggctttacag gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg
     8821 atcggtttcc cagcctttac atttcaggat ccagccaggc tgaccgccac cacctgcatt
     8881 accaccgcgg ggaactgcat caccaaatgt accattcgga tactgcgggt attcttccag
     8941 ataacccaat ttatcgagac gctgctggtc acgtaatttc acgcggcaaa ggttcaatac
     9001 ggcatcctgc attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa
     9061 agcagcaagt aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc
     9121 gcttaccagc ggcggaccat cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg
     9181 agcaacgttt tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat
     9241 cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg
     9301 atcaatggcc cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc
     9361 cattacctct ttgccttccg ctgttttggt atttaactca atagaacgtt tgttactgtt
     9421 aagcatggtg aagtaaagcg catcgatatc aggaatatct cgcagttggt gacgcgttac
     9481 gtcaccaacg ccgggacgtt caattttaat aacgtcagcg ccaaaccagg ccagcatttg
     9541 agtacaagat gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg
     9601 agttgacata gcaataccta ttagttaatt aaatatgtgt taatttagc
//
LOCUS       DAZPFP010000143         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPFP010000143 DAZPFP010000000
VERSION     DAZPFP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.144"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUR4_003131"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUR4_003131"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1456615.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUR4_003132"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUR4_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1456616.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUR4_003133"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUR4_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHUR4_003134"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHUR4_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1456617.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHUR4_003135"
     tRNA            4965..5038
                     /locus_tag="ACHUR4_003135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHUR4_003136"
     CDS             5173..6783
                     /locus_tag="ACHUR4_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1456618.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHUR4_003137"
     CDS             6815..7234
                     /locus_tag="ACHUR4_003137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456619.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHUR4_003138"
     CDS             8353..9099
                     /locus_tag="ACHUR4_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1456620.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHUR4_003139"
     CDS             9397..>9537
                     /locus_tag="ACHUR4_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456621.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZPFP010000144         9512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPFP010000144 DAZPFP010000000
VERSION     DAZPFP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.145"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..276
                     /locus_tag="ACHUR4_003140"
     CDS             <1..276
                     /locus_tag="ACHUR4_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1456622.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="ACHUR4_003141"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="ACHUR4_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1456623.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="ACHUR4_003142"
     CDS             2098..3417
                     /locus_tag="ACHUR4_003142"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1456624.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3794..4864
                     /gene="dosC"
                     /locus_tag="ACHUR4_003143"
                     /pseudo
     CDS             3794..4864
                     /gene="dosC"
                     /locus_tag="ACHUR4_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4864..5481
                     /locus_tag="ACHUR4_003144"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="ACHUR4_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHUR4_003145"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHUR4_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHUR4_003146"
     CDS             6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHUR4_003146"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1456625.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="ACHUR4_003147"
     CDS             6978..7952
                     /locus_tag="ACHUR4_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1456626.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="ACHUR4_003148"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="ACHUR4_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHUR4_003149"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHUR4_003149"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1456627.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2320 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca ag
//
LOCUS       DAZPFP010000145         9440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPFP010000145 DAZPFP010000000
VERSION     DAZPFP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.146"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHUR4_003150"
     CDS             <1..1154
                     /locus_tag="ACHUR4_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456628.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHUR4_003151"
     CDS             1833..2051
                     /locus_tag="ACHUR4_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1456629.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUR4_003152"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUR4_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1456630.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUR4_003153"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUR4_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1456631.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUR4_003154"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUR4_003154"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1456632.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHUR4_003155"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHUR4_003155"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1456633.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHUR4_003156"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHUR4_003156"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1456634.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHUR4_003157"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHUR4_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1456635.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2314 c         2446 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gtcgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAZPFP010000146         9386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPFP010000146 DAZPFP010000000
VERSION     DAZPFP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.147"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="ACHUR4_003158"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="ACHUR4_003158"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1456636.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="ACHUR4_003159"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="ACHUR4_003159"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR1456637.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="ACHUR4_003160"
     CDS             3116..3400
                     /locus_tag="ACHUR4_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1456638.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHUR4_003161"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHUR4_003161"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1456639.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHUR4_003162"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHUR4_003162"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1456640.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHUR4_003163"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHUR4_003163"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1456641.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="ACHUR4_003164"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="ACHUR4_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1456642.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2238 a         2480 c         2390 g         2278 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggctctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagcg actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atatta
//
LOCUS       DAZPFP010000147         9378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPFP010000147 DAZPFP010000000
VERSION     DAZPFP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.148"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUR4_003165"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUR4_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1456643.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUR4_003166"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUR4_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1456644.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUR4_003167"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUR4_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1456645.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUR4_003168"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUR4_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1456646.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUR4_003169"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUR4_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1456647.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUR4_003170"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUR4_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1456648.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUR4_003171"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUR4_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1456649.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUR4_003172"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUR4_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHUR4_003173"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHUR4_003173"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1456650.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..>9378)
                     /gene="mdh"
                     /locus_tag="ACHUR4_003174"
     CDS             complement(8442..>9378)
                     /gene="mdh"
                     /locus_tag="ACHUR4_003174"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1456651.1"
                     /translation="KVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVD
                     LSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQ
                     QVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAEL
                     KGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAG
                     GGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEE
                     RKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
BASE COUNT         2250 a         2403 c         2406 g         2319 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttc
//
LOCUS       DAZPFP010000148         9153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPFP010000148 DAZPFP010000000
VERSION     DAZPFP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.149"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHUR4_003175"
     CDS             <1..923
                     /locus_tag="ACHUR4_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1456652.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUR4_003176"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUR4_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1456653.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUR4_003177"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUR4_003177"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1456654.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUR4_003178"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUR4_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1456655.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHUR4_003179"
     CDS             4446..4805
                     /locus_tag="ACHUR4_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1456656.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUR4_003180"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUR4_003180"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1456657.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUR4_003181"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUR4_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1456658.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHUR4_003182"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHUR4_003182"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1456659.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2210 c         2473 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atg
//
LOCUS       DAZPFP010000149         9122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPFP010000149 DAZPFP010000000
VERSION     DAZPFP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.150"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(150..1361)
                     /locus_tag="ACHUR4_003183"
     CDS             complement(150..1361)
                     /locus_tag="ACHUR4_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1456660.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            complement(1633..1727)
                     /locus_tag="ACHUR4_003184"
     tRNA            complement(1633..1727)
                     /locus_tag="ACHUR4_003184"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1691..1693),aa:Sec,seq:tca)
     gene            2020..3403
                     /locus_tag="ACHUR4_003185"
                     /pseudo
     CDS             2020..3403
                     /locus_tag="ACHUR4_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            3413..5731
                     /gene="yicI"
                     /locus_tag="ACHUR4_003186"
     CDS             3413..5731
                     /gene="yicI"
                     /locus_tag="ACHUR4_003186"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1456661.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5784..7493)
                     /locus_tag="ACHUR4_003187"
     CDS             complement(5784..7493)
                     /locus_tag="ACHUR4_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1456662.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(7614..9005)
                     /gene="xanP"
                     /locus_tag="ACHUR4_003188"
     CDS             complement(7614..9005)
                     /gene="xanP"
                     /locus_tag="ACHUR4_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1456663.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
BASE COUNT         2236 a         2246 c         2345 g         2295 t
ORIGIN      
        1 atttaaaaat gtcaatttgt ttttaattat gtttttgtaa taagtttgaa gacgcaaaaa
       61 aataatataa taacaaaaat cctccagcct tgtaggccag aggataaaat acttaattga
      121 ggaaaacatc agggaaaagt atttctgact taactttccc ggctggagag tgaatcaatg
      181 taatcagcgt accattgcat catttcacgg cggccatcga gatacagggc atgattatag
      241 gtacccctga tggaattttt atccacatga gctaactgca tttcaatcca ggcggaatta
      301 aagccttgtt catgcagaat tgtactcatg gtgtgtctga aaccgtgccc tgtgagtctt
      361 cccttatatc caagtaattc aattacttgg ttaatgcttt ccttactaat tggtttgcgg
      421 ggatcatttc taccgataaa gactagggga tgatgttttg agattggttc aagctgctta
      481 aatagcataa tgacctgctc agagagcggc acaatatgag gtcgcttcat cttcattact
      541 tctgccggga tctcccacaa ttttgtctca aaatcgatat cttcccagcg tgcaaaacgc
      601 agttcttgtg ttcgtacgcc ggttaacata atgatctgag tagctgtctt agtgattata
      661 cttccggtat aacccgccag atcgttgagg aagtggggaa gttcattagc agtaagaaag
      721 ggaaaatgta cttttttcgg tgtagctaaa gcactggcaa gatcgggggc tgggttatag
      781 tctgcccggc cagtaacaat tgcatagcgg aacacctcac cacagcgctg gcggaccttg
      841 cgcattttct ccagagctcc acgtttttcc attttacgca gtgcttccag taacttcatt
      901 ggtttgattt cggcaatagg gcgcttgcca atgtagggaa aaatgtcttt ttcaaaggta
      961 tcaatgattt catcacgata gcgtaatgac caccgatcag cttttaactg gtgccattcg
     1021 cgagcaatag cctcgaacgt atttttcaga ctcatttgct gcgcgatttt ttcttccttt
     1081 tttgcctccc ctggatcgcc tccagcagcg aggagtttcc tggcttcact gcgtttctcg
     1141 cgagcatcgg ctagcgttac atcggggtat acgccaatag aaagcatctt ttctttacca
     1201 gcaaagcggt acttcatgcg ccagtattta gaaccattag tgttaatcag tagatacata
     1261 ccgccgccat cagcaagctt atagggtttg tctttgggtt ttgcggtttt cactttgata
     1321 tcgttaatgc catgacatcc tccggctttt gatgaattca tgttcctgag ggggatttct
     1381 gccagtaact aacctgatac ccccaataca ccccttgtat aacaaagata ttgttaaaag
     1441 ccgttgttat gtatcacatt tttcctatta tgggggtgct tgtatttcaa taggaaataa
     1501 caccccctaa agtaccccca aaagaaactt gatacggggt gaagtaggtt gacctcagga
     1561 tacaaataaa tactgatata caagggatta gaagagattt attgacattg ggagagttca
     1621 gaagaggtaa actggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc
     1681 caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc
     1741 tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt
     1801 tttttccgcc taataaacgc agcgtattat cacgaaagca ttataattta tataaaaccc
     1861 atattttaca atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc
     1921 ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt
     1981 tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaggagtga agtgttgtcc
     2041 gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac
     2101 gtaatgttat atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc
     2161 ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg
     2221 ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca
     2281 ctgccattcg ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacggc
     2341 aaaatgatct atgcagcaat tacttacacc ctaacttacc ttactttata ccgtcgtcaa
     2401 tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct
     2461 gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctttctactg ttctgatgat
     2521 gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg gcggtatcgt
     2581 ggtcctttcc gtggtggcat tcatgatgct ggcattttgt ttcttcacca ctaaagaacg
     2641 cgttgaagca ccacctacaa caacgtctat gcgggaagat ttacgtgata tctggcaaaa
     2701 cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctggcgg tgtgcgtacg
     2761 cggtggggcg atgatgtatt acgtcacatg gattttgggc acgccggaag tgtttgtcgc
     2821 ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa aacctctgac
     2881 cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag
     2941 cctcgcgatg ttctttgttc ccatgcaggc cagcatcact atgtttgtct tcatcttcgt
     3001 gattggtgtg ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt tcgataccgt
     3061 cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg ctggcacgct
     3121 gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga tgctggctta
     3181 tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca tcattattgc
     3241 gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg ctaaacgcta
     3301 ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggctc agggtaaacg
     3361 ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg caatgaaaat
     3421 tagcgatgga aactggttga ttcaacctgg cctcaatttg attcacccgc ttcaggtatt
     3481 cgaggttgaa cagcaggata atgaaatggt ggtctatgct gccccccgtg atgtgcgtga
     3541 acgtacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc cacaggaagg
     3601 tattgtcggt gtgcggattg agcattttca gggggcgctg aataacggtc ctcattatcc
     3661 gctcaatatt ttgcaggacg tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt
     3721 taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac tggattttct
     3781 gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg tgcaggacac
     3841 gaataatcaa cgcaattata tgtttgagcg gcttgatctt ggcgttggcg aaacagttta
     3901 cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag agacctggaa
     3961 ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct acatgactaa
     4021 ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc tcttttgaag tgggatcgga
     4081 gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact ttgttatcga
     4141 cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc tttactggtc gtccggcgct
     4201 gccgcccccg tggtccttcg gcctgtggct aaccacttca tttaccacca actacgacga
     4261 agcgacggta aacagcttta tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt
     4321 ccactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt gggacccgct
     4381 gactttccct gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg
     4441 cgtctggatt aacccctata tcggtcaaaa atcccccgtc tttaaagagt tacaagagaa
     4501 aggctattta ctcaaacgcc cggacggttc gctatggcag tgggataaat ggcagccagg
     4561 tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa
     4621 aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac gtatcccaat
     4681 tgatgttcag tggtttgacg gttccgattc gcagaaaatg cataaccatt atgcgtacat
     4741 ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt
     4801 gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg gtacactggg gtggcgattg
     4861 ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc
     4921 aggttttggc ttctggagcc acgatatcgg cggctttgaa aataccgctc cggcgcacgt
     4981 ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac acggtagcaa
     5041 atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtggtgc gcttcttcac
     5101 gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgcgcgtg cgaacgcgcg
     5161 gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg cttgtgatta
     5221 ccttgaccgt caatacatgt taggcgacaa cgtgatggtt gcgccggtgt tcactgaagc
     5281 gggcgatgtg cagttctacc tgccggaagg tcgctggaca cacctgtggc acaacgatga
     5341 actcgacggt agtcgctggc ataaacagca gcacggcttc ctgagtctgc ccgtttatgt
     5401 gcgtgataac actctactgg cgctgggcaa caacgatcaa cgtcccgatt acgtgtggca
     5461 cgaaggcacg gcattccacc tctttaatct gcaagacggg catgaagccg tctgtgaagt
     5521 gcctgctgct gacggatcgg tgatctttac tttaaaagca gcacgtactg gcaacacgat
     5581 tactgtgact ggtgcgggcg aggcgaagaa ctggacgctg tgcttgcgca atgttgtgaa
     5641 agtaaatggt ctgcaaggcg gttcgcaggc tgaaagtgag caggggctgg tggtgacgcc
     5701 tcaagggaat gcgctgacaa ttacgttgta atctattgac ggaatgccgg gtacgtctga
     5761 gtcgtacccg gcgagattga tgtttaccgc accggctccg tcgatgtaac ttcaccgctg
     5821 gaaacgacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcgtt cacggcatgc
     5881 aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc actggcggta
     5941 agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg ttgcaaaata
     6001 ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg cgtttgtgaa
     6061 acacttgcgg tggcttccag aatgcctttt tcagtaaatg cactcagttc gctgatattc
     6121 accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac acgattgaat
     6181 gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt atgatcggta
     6241 accagcgtca ggttggcacc gtaaccatcg agcgcggtga gctgccacgg gaagtcaggg
     6301 tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag gctgtttaac
     6361 caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt atattccagc
     6421 ccggcaattg ccgcatcatc aaggatcaac gttttaccgt cacgcagcca gttccctgac
     6481 gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac gccctgcggg
     6541 gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat gaactcgctg
     6601 gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg
     6661 cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcagacg agcatcgatg
     6721 acttccaagc gaccaatctg caacgacggg acagagcgta atggcgcaaa gaagtcggtt
     6781 agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac ttgccagctg
     6841 ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg
     6901 ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt ggtggcagga
     6961 acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt acccagcact
     7021 ttaccggctt ctggtgacca cggaaccacg ccgccattta cccgctgcgc gctcagtttc
     7081 cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc ggctttgaac
     7141 ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag gatggtatcg
     7201 acatggcgtg gttcggtcag ttggcgactg cttagcgcaa tgtcgacact ttttgccacc
     7261 aggggcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat atgagatggc
     7321 gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt ctcggaaacc
     7381 catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag gccagcgatc
     7441 accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt catggtcttc
     7501 catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc tcaaggcggg
     7561 aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt gaattactgt
     7621 ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc ggcgatcccg
     7681 gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg ctgctgagac
     7741 acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg gttcagcggc
     7801 tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac aagcgttgcg
     7861 ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc
     7921 agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac accagtcaac
     7981 tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa caccgccgaa
     8041 acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat gtacagcgga
     8101 ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat ggtttccagc
     8161 gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat gccaagaccg
     8221 taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt gctttctggc
     8281 aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaattac cagtgaggcc
     8341 acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa aaccacgcct
     8401 gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg
     8461 ccaatggagg tcagccccac ctgaattagc gacaggccga taatcatcac cacaacgcca
     8521 gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat caccatctcg
     8581 gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg aacatcagca
     8641 ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt gaagctggtg
     8701 ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg aataatcgat
     8761 gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa
     8821 cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat cgccagcaga
     8881 tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag acggtaaatc
     8941 agttcgctgt tttgagtctg agcaaccggt tgcgcatttt ctgactcgag ggtggaaaca
     9001 gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga aagagaaaag
     9061 caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact tttttatata
     9121 ac
//
LOCUS       DAZPFP010000150         9043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPFP010000150 DAZPFP010000000
VERSION     DAZPFP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.151"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            116..1459
                     /locus_tag="ACHUR4_003189"
     CDS             116..1459
                     /locus_tag="ACHUR4_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1456664.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1684..3339
                     /gene="opgD"
                     /locus_tag="ACHUR4_003190"
                     /gene_synonym="mdoD"
     CDS             1684..3339
                     /gene="opgD"
                     /locus_tag="ACHUR4_003190"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1456665.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3479..3703
                     /locus_tag="ACHUR4_003191"
     CDS             3479..3703
                     /locus_tag="ACHUR4_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1456666.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3766..4302
                     /gene="rimL"
                     /locus_tag="ACHUR4_003192"
     CDS             3766..4302
                     /gene="rimL"
                     /locus_tag="ACHUR4_003192"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1456667.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4297..5277)
                     /gene="ydcK"
                     /locus_tag="ACHUR4_003193"
     CDS             complement(4297..5277)
                     /gene="ydcK"
                     /locus_tag="ACHUR4_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1456668.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5401..6393
                     /gene="tehA"
                     /locus_tag="ACHUR4_003194"
     CDS             5401..6393
                     /gene="tehA"
                     /locus_tag="ACHUR4_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1456669.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6390..6983
                     /gene="tehB"
                     /locus_tag="ACHUR4_003195"
     CDS             6390..6983
                     /gene="tehB"
                     /locus_tag="ACHUR4_003195"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1456670.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            7286..7942
                     /locus_tag="ACHUR4_003196"
     CDS             7286..7942
                     /locus_tag="ACHUR4_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR1456671.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(7960..8466)
                     /gene="tnpA"
                     /locus_tag="ACHUR4_003197"
                     /pseudo
     CDS             complement(7960..8466)
                     /gene="tnpA"
                     /locus_tag="ACHUR4_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8474..9043
                     /locus_tag="ACHUR4_003198"
                     /pseudo
     CDS             8474..9043
                     /locus_tag="ACHUR4_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         2318 a         2120 c         2251 g         2354 t
ORIGIN      
        1 agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttcccgc
       61 cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag acactatggc
      121 gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta
      181 ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg tgaatctggc
      241 tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagacg agctggcgcg
      301 actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct
      361 tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc
      421 aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc tggcgcgtaa
      481 tcccttccgc gtttttacat ccttactccg ccttgagctt atcgagaacg aaattttgcg
      541 ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact
      601 gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg agtttgtgca
      661 ggaagccctg gaaacgtttc gctggcacca gttagcaacg gtagatgaag aaacctatcg
      721 cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat
      781 caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga tgcctgaatg
      841 cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac cgattttact
      901 acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg
      961 cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa cgccgaaagg
     1021 gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata atctcactca
     1081 ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca
     1141 gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc atcgtcaggc
     1201 gattcatcct ggagacgatc cacagccctt aattgaacgt ggttgggtag tggcgcaacc
     1261 catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg
     1321 taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg agcaggcgtt
     1381 gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac gcagtaaacg
     1441 tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac
     1501 aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca tattctctca
     1561 gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt
     1621 tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga ctcactttca
     1681 ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc
     1741 agcggcattg cttctctttt ttctcaggca gcattcgcgg cagattctga tattgccgac
     1801 gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa
     1861 acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat gacgccgcag
     1921 gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt
     1981 caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt
     2041 tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa
     2101 tacaacgatg caggtgttga taccaaacaa ttagaagggc aaagcgatct cggctttgcc
     2161 ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc atttctcggc
     2221 gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc ccgcggcctg
     2281 gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc cttctggttt
     2341 gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga tagcgccagc
     2401 attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat tatggatgtg
     2461 gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcatttcgcc gatgaccagt
     2521 atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc gcaaattcat
     2581 gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaat
     2641 aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta
     2701 ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta taacaaacgc
     2761 ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa
     2821 atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc agaaaaagct
     2881 gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct
     2941 gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa
     3001 ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc cgtcgatttc
     3061 gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat tacgctttcc
     3121 agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg ttatcgtatt
     3181 cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg gatgtatcta
     3241 cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc gccagcgccg
     3301 gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct tcggcacctt
     3361 cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct aagttaaatg
     3421 agtaatctga tggtgtgtat ttcagataca ccgtgtcagc cactaacagg gagtgcgtat
     3481 gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc
     3541 cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg
     3601 tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga
     3661 gatgctcaaa atcctgcagc aggagagcgt caaagaggtg taaactttcc taagccgcca
     3721 gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac tgaaacgata
     3781 aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac acctctttat
     3841 cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagttcgtt
     3901 cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc
     3961 tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcgtttaat
     4021 cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga atctcatcag
     4081 gggcagggga tcatttctca ggcgctgcag acattgattc atcattacgc tcagtctggt
     4141 gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtcgct
     4201 ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct gaatgatgcc
     4261 tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg ccagcggcgt
     4321 tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc
     4381 atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa ttatcaccgc
     4441 acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa tcggtccacc
     4501 acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac
     4561 aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat cttcttctat
     4621 gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc agacgttgtt
     4681 ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt caataaaagc
     4741 ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac
     4801 ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc cttgtgcggc
     4861 gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt gcacgtaaac
     4921 taatgagtca tgaatggtga cactgtcact tatatatgca ccctggctga tttcactatt
     4981 atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg tgccggtgat
     5041 acgcgtatta ccggaaatca ccgttccgcc gaacgcaata gcattctggt cgtaaatcca
     5101 gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cggttcctgc
     5161 tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacac tttttttagt
     5221 gccatcttcc tgataactaa aggctcgctg ttcttcactt aggcgatatt tgcgcatagc
     5281 gttttcccac aggtgactta ctataaccgt agcaaattct gcggctctgg ctatgctcga
     5341 gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat gagtaagaag
     5401 atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt gttggggacg
     5461 atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag ccactggtta
     5521 ggggatgggc tggtgattct ggcgatgatc atctggggat tattgactag cgcatttatt
     5581 acccgactca tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc agtgctgagc
     5641 agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt tgttccgtgg
     5701 tttcgcccac tggcggtgtg catgttcagt tttggtgtcg tggttcagtt ggcttatgcc
     5761 gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac cacgcctgga
     5821 ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc
     5881 tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct aagccttgaa
     5941 ccggtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact gcggacatca
     6001 ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggctgag cgtcaacggc
     6061 ggcgagggtg acacgctggc gaaaatgctt ttcggttatg gactgctgca actgctgttt
     6121 atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt ctggagtttc
     6181 tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg cagcgataat
     6241 ggatttttcc atacgctggc ggtgccgctg tttatcttta ccaattttat tattgcaata
     6301 ctgctcatcc gtacttttgc gcttctgatg cagggaaaat tgttagtcag aaccgagcgc
     6361 gccgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac tattttactg
     6421 ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa gtggttaaac
     6481 cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac ctggcagcca
     6541 atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaac gtcgagcgca
     6601 ttaaatccat tgaaaatctg gataatttac acacccgggt cgttgatctg aataacctca
     6661 catttgatgg acagtacgat tttattcttt cgactgtggt gctgatgttc cttgaggcta
     6721 aaaccatccc agggttgatt gccaatatgc aacgttgcac taaacctggt ggttacaacc
     6781 tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc acgtttgcct
     6841 tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa tacaatgaag
     6901 acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg cgtttcgcca
     6961 cgatgctgac acgtaaaaaa tgacccggta agcacaaaac gcgtgaaaat tccccacgct
     7021 gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg atgtgaaatt
     7081 aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact actgatgatt
     7141 gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa aaatttattt
     7201 tcaccaacac tttttttgcc acaacacgaa gcggcgcttt ttgctataac ttagaaagta
     7261 atataatcat ctcaggaaac tattcatgcg taccacatca tttgcgaaag ttgcagcttt
     7321 atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc acccagccag ataaatataa
     7381 caattactct gatttaaaag aaacaacctc agcaacaggt aaacctgttt tacgttgggt
     7441 agacccgagt tttgatcaaa gcaaatatga cagcatcgtc tggaacccaa tcacttatta
     7501 tccggtacca aaaccttcta cccaggtagg gcagaaagtt ctggataaaa ttttgaacta
     7561 taccaacacc gaaatgaaag aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg
     7621 gccgcgtagt ctgattttcc gtggggccat taccggtgta gataccagca aagaagggct
     7681 gcaattctat gaagtggttc ctgttgcatt agtggttgcg gggacgcaaa tggctacagg
     7741 ccaccgtacc atggatactc gcctctattt tgaaggtgag ctgattgatg cagcgactaa
     7801 taaaccggtt atcaaagtcg ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac
     7861 gccaatggct ttcgaaaata ttaaacaagt tattgatgac atggcgaccg atgccaccat
     7921 gtttgacgtt aacaaaaagt agttccagac gcgccatcgt tagatggcgc tttttatccg
     7981 gtgcgccgta aaaccccatc cttcagggcg gggatataag gcgcggtttt ccacctgacc
     8041 aggtgtttgc tgttgttcaa tgtattggcg gatgacggat attggcgcac ctccgcagct
     8101 actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga ctgcaatatc
     8161 tggtcgatct cgtcgcagta acctacccga tacgcctttg aggctgttta ccagactgga
     8221 tatcgccagt ttgggaggtt agttaattaa caaatggacg tgatctggtt cgccatccat
     8281 ttcaaccagt tcagcttcaa aataagcaca tacatttgaa aagtaagtgc gtagtttttc
     8341 tgtcgcgtca tggtcaaaaa tctggcgtcg gtatctggtg acaaagacta ggtgaacatg
     8401 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca cagaccaaag
     8461 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac caggtggtca
     8521 acaggagtgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
     8581 ggcacgtcag aatgagaatc ataaggccgg taataaatac atcccttacg ggaaaatggc
     8641 ttcctggctg gttgagtgga aaaatgccac tgaaacgcaa tggcttaaag attctccctc
     8701 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccggaa
     8761 gcgggctgct tttccccgat tcaaaaagcg gtgacagaat gatgcattcc gctacccgca
     8821 gggcgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaactgg gctggatgcg
     8881 ctaccggaat agccgtcagg tcacgggtgt tgtgaaaaat gtcactgtca gccagtcctg
     8941 cggtacgtgg tacatcagta ttcagacaga aagtgaagta tccactccgg ctcacccttc
     9001 agcatcaatg gtcgggctgg atgctggcgt ggctaaactc gcc
//
LOCUS       DAZPFP010000151         9029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPFP010000151 DAZPFP010000000
VERSION     DAZPFP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.152"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUR4_003199"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUR4_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1456672.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUR4_003200"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUR4_003200"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1456673.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUR4_003201"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUR4_003201"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1456674.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUR4_003202"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUR4_003202"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1456675.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUR4_003203"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUR4_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1456676.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHUR4_003204"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHUR4_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1456677.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHUR4_003205"
     CDS             6064..6606
                     /locus_tag="ACHUR4_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1456678.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUR4_003206"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUR4_003206"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1456679.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUR4_003207"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUR4_003207"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1456680.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHUR4_003208"
     CDS             8003..8260
                     /locus_tag="ACHUR4_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1456681.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHUR4_003209"
     CDS             complement(8236..8790)
                     /locus_tag="ACHUR4_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1456682.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZPFP010000152         8953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPFP010000152 DAZPFP010000000
VERSION     DAZPFP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.153"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(86..1450)
                     /gene="tdcG"
                     /locus_tag="ACHUR4_003210"
     CDS             complement(86..1450)
                     /gene="tdcG"
                     /locus_tag="ACHUR4_003210"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1456683.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1522..1911)
                     /locus_tag="ACHUR4_003211"
     CDS             complement(1522..1911)
                     /locus_tag="ACHUR4_003211"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1456684.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1925..4219)
                     /gene="tdcE"
                     /locus_tag="ACHUR4_003212"
     CDS             complement(1925..4219)
                     /gene="tdcE"
                     /locus_tag="ACHUR4_003212"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1456685.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4253..5461)
                     /gene="tdcD"
                     /locus_tag="ACHUR4_003213"
     CDS             complement(4253..5461)
                     /gene="tdcD"
                     /locus_tag="ACHUR4_003213"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1456686.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5487..6818)
                     /gene="tdcC"
                     /locus_tag="ACHUR4_003214"
     CDS             complement(5487..6818)
                     /gene="tdcC"
                     /locus_tag="ACHUR4_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1456687.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6840..7829)
                     /gene="tdcB"
                     /locus_tag="ACHUR4_003215"
     CDS             complement(6840..7829)
                     /gene="tdcB"
                     /locus_tag="ACHUR4_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1456688.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7928..8866)
                     /gene="tdcA"
                     /locus_tag="ACHUR4_003216"
     CDS             complement(7928..8866)
                     /gene="tdcA"
                     /locus_tag="ACHUR4_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1456689.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         2348 a         2342 c         2077 g         2186 t
ORIGIN      
        1 aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac
       61 ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc agtcctccgc
      121 gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca
      181 ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta
      241 ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa
      301 ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct
      361 gcgccggact gccgcccagt agttcagtta accctgccgc tgccattgaa caggccacgc
      421 caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt ttatacagcg
      481 cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt accggacgac
      541 ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg
      601 ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatgttga
      661 tccagtcgat gacattcatc ggatcgttag agatgttatc gctagaaacc agctgacgac
      721 gcagcgctac ggcacgacac ggcacattga gcggaccagg cagcacgcct tcagtgttca
      781 tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct gcgtcaattt
      841 ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt
      901 tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt acggacgttt
      961 cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt
     1021 aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc attccgttct
     1081 catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa
     1141 tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct ataaatgcag
     1201 ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg ataatggcga
     1261 catccgtggc atggcctttg cccgtcagtg acaacgaccc gtacagatcg accacaatat
     1321 ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat
     1381 tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg
     1441 cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc
     1501 gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa tttccagctt
     1561 cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg tcgcctgatg
     1621 ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat
     1681 gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca cgatcgcttt
     1741 gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct caccggtctg
     1801 cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac
     1861 ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc
     1921 ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt tcacgggtca
     1981 gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg
     2041 ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac
     2101 cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt
     2161 tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg tacgagatcc
     2221 catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcacctt
     2281 tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac
     2341 cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata gtcagaatcg
     2401 actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc ataaagcgtt
     2461 caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac tgcggatatt
     2521 caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt ttcacgcggg
     2581 catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca
     2641 tagtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag ctgtacttgt
     2701 cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc cagtccatga
     2761 agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc agcggtgctg
     2821 ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt gcgtagagca
     2881 gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca atcaccattg
     2941 ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt
     3001 cattttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat tttttgaagg
     3061 caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc ggccccatag
     3121 tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac cagcgtacga ccgtccagcc
     3181 ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt
     3241 ccggtgtacg caggaagcgt accatacgga tcttcatgat gaagtgatcg atcagttcct
     3301 gtgcctgctg ctcattgagg acgccagctt taaagtcgcg ctcaatgtag atatccagga
     3361 acgatgccgt gcgccccagc gacatcgcgc cgccattttg cgatttcact gccgccagat
     3421 aagcgaagta gagccactgc accgcttcct gtgcattctg cgccgggcga gagatatcaa
     3481 agccatattt cgccgccatt tcctgaatct gcaacagcgc atgacgatgc tctgccagct
     3541 cctcacgcag acggatggtg gcttccagat cctcgccttt ttccagacga gactggagat
     3601 cggcaaattg cagttcgcgt tcacgcacca gataactgat gccatacagc gctacgcggc
     3661 gatagtcacc gataatgcgc ccacgaccat agccatctgg taaaccggtc agcacgccag
     3721 atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt
     3781 tacgcagatc ggtaaacaga tattcaaatt cactgtccat ttcacggcca taggcgtgga
     3841 atgaactttt aatcatatta atgccaccga acgggtgtag cgcacgtttc aacggcgcat
     3901 ccgtttgcag gccaacaatt ttttccagcg gctggttaat atatcccgca tcatgagcgg
     3961 taattgtggt ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg ttttcgatac
     4021 ggatgccttc cattaccttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagaa
     4081 aagattcatc gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt
     4141 cgcttttcca gtccgtacct ttaaagccaa gccatgcgtc agcgtacagc ttatcgctgg
     4201 tatcaatatc taccttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa
     4261 attctgcggg cgcgttaact ttacctaaat gaatggcatc caaagcaatc attttttctt
     4321 cgttagtcgg aataacgacg caaatgacat gcgcattttc actggaaaca attcgctcgc
     4381 caaaagagtt agaacgatta ttcatttctg tatcaatctc tacgcctaat acagccaaat
     4441 gttccatgac cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa
     4501 ttccatccag ccgacgtaat gaagctgcat gtccggcaat atgacgggca attcggtgaa
     4561 caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa
     4621 cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc cgactcttta ttcactacgc
     4681 gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc gcaccaaagt
     4741 cgacatcgcc actgcgggta cccatcatca agccttccag cggcgtcatt cccattgagg
     4801 tgtcaacact ctgaccgttg cgaactgcgc agattgacgc gccattgcca agatgcgcca
     4861 caaccaggcc ggagtcatct tccgccagat tcagcagcga atgtgcgcgc tgggaaacat
     4921 agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc
     4981 acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata
     5041 ccgccacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaatttg
     5101 cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg
     5161 taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg
     5221 ccacgctgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt
     5281 cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt
     5341 ccgagttaat accgtcggca atacctgaca ttaatacttc acaatcgctg gcatcgagta
     5401 cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca
     5461 tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt caggatggtc
     5521 agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac
     5581 ggcgctttac ggatagcata catcggcaac aggcacagca gggatgcgat aatcggtgcg
     5641 cccatggctt caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag
     5701 cccatgatga agatcatgct gagagtattc agtttaccca gcgacacttt ggttttgtca
     5761 cctttataac cgaacttcag aatcagacca ttcaagcctt ccagcgtccc cagatagtga
     5821 ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta
     5881 atcgcgaacg ttgttttcgt accggtcatg gacgcaaagt ggttagccag ataagaaagc
     5941 actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagagt aaacaggcag
     6001 ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg
     6061 gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa
     6121 accacgaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc
     6181 agccagacag tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc
     6241 tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg
     6301 gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag
     6361 atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca
     6421 aatccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc
     6481 cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta
     6541 ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac
     6601 agacgcgcca gcgcccggtg gcaataaaat gcgatggggt atgccaatac caacatcaga
     6661 agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg
     6721 attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt
     6781 tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat
     6841 taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa
     6901 ataatactga cggtttttct attttgaata tattggtcta atttaccgct taataatgca
     6961 gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc
     7021 atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg
     7081 atttcgtaag ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta
     7141 gttcggtgcg tggttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttct
     7201 gactgtacgc caataacacg aatggtcggg ttaatagatt taattgccac cgcaatacca
     7261 gcaattaaac caccaccacc aattggcaca atcacgttat cgacatcata gagatcttcc
     7321 ataatttcca gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg
     7381 ataaaaatac gaccttccat ttcgacaatt tcgctcactt tagcgatagt gtcgttgaag
     7441 ttatcaccat gcagaacgac ttctgcggag tagtcgcacg ttgccgctac tttggatttt
     7501 ggcgcacctt ttggcatcac cactttaccg tcgataccca gcatcgcgca ggagagcgaa
     7561 accccttgcg catggttgcc cgcagaacag gccaccacgc ctttgcgttt ttccgcatcg
     7621 gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaacccgt acgctgcata
     7681 ttttcaaact tcaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc
     7741 atgcctgttt tataaattcg cccagccagt cgttgtttcg cttcaataat atcatcaata
     7801 gcaaccggca gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa ttaaatatgt
     7861 aggtaaccgt aaccgacacc tgcaaaacag acaggtggat tatttatgtt taaataaaaa
     7921 caaacaacta accaacttct attaattgcc ttcgtctaca cccattataa gatgaatact
     7981 ctttggctaa ttccaccaaa accgatgctg cttttttaat acgataattt ttcgaccata
     8041 ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata aattgattag
     8101 aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa
     8161 gattataaat tgtcacgact gagtcggttt taacgatgtt ttcaatactg atgccatttc
     8221 tttgtaacgt agtaagcagt tcgctgtagt accccatatt agtttgtggc aacacccact
     8281 gttcgttctt caacgactcc agcgtggtgg tgccggtgca tgttcgggac ttactggcta
     8341 ccagcacaaa ctcggactcg aacagcggct caacatgtaa atcctgaagc ttcatttctg
     8401 cacttaacgt accaatcgca aaatccagtc gaccgtcgcg gattgccggt aagaacgaag
     8461 acagttgcgc ttcatacata gaaacctgcg ctttcgggaa cacctctttg aacttgttga
     8521 tcatccctga cataaaagta aaaccaatca atgaaggaaa accaaatgag acttccacca
     8581 ccgcttcaga agacataccg cttatctcat taaccatatt tttcatttca cgggtaatgg
     8641 attcggaacg ggagagtaac aattgaccgg caggtgttaa tgttacaccg gtatttttcc
     8701 gcaccactaa ttccacacca aaataatctt caatatcgtt aatgatttta ctgacggccg
     8761 gttgagttaa ccctaattct tttgcagccg agccgataga gccacttcta atgacttcct
     8821 gaaagactac caggtgctgc gttttcggaa gaagaatagt gctcataacg acctacgtta
     8881 attacctcat tgacggcatg aagtgtatca aaatgaaata aacaggatat gtgcgaccac
     8941 tcacaaatta act
//
LOCUS       DAZPFP010000153         8907 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPFP010000153 DAZPFP010000000
VERSION     DAZPFP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.154"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            63..2247
                     /gene="espL4"
                     /locus_tag="ACHUR4_003217"
                     /pseudo
     CDS             63..2247
                     /gene="espL4"
                     /locus_tag="ACHUR4_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2216..3953)
                     /gene="aceK"
                     /locus_tag="ACHUR4_003218"
                     /pseudo
     CDS             complement(2216..3953)
                     /gene="aceK"
                     /locus_tag="ACHUR4_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4137..5441)
                     /gene="aceA"
                     /locus_tag="ACHUR4_003219"
     CDS             complement(4137..5441)
                     /gene="aceA"
                     /locus_tag="ACHUR4_003219"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR1456690.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5471..7072)
                     /gene="aceB"
                     /locus_tag="ACHUR4_003220"
                     /pseudo
     CDS             complement(5471..7072)
                     /gene="aceB"
                     /locus_tag="ACHUR4_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7341..8270)
                     /gene="metA"
                     /locus_tag="ACHUR4_003221"
     CDS             complement(7341..8270)
                     /gene="metA"
                     /locus_tag="ACHUR4_003221"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1456691.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8427..8870
                     /locus_tag="ACHUR4_003222"
     CDS             8427..8870
                     /locus_tag="ACHUR4_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1456692.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2332 a         2151 c         2155 g         2269 t
ORIGIN      
        1 taaatttttt cactgttctg atagttaaaa ttcaagacat caataaacaa tgagatattt
       61 aaatgattac tcgtattcct cgtagttctt tctctgcaaa tattaataat acagcccaga
      121 caaatgaaca ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg
      181 gcaaggagct ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatgttc
      241 gtcatatctt tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg
      301 ataagaaaga aattacgatg attaataaaa acgaaaacac gacactcact caaaccattg
      361 ctacaatatt tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata
      421 aacaagaatt aaaccaaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta
      481 atggtcaatg ttatttcccg gggcgcccca aaaccgtata gtataccgac acattgctgc
      541 acaatatatt aatgatattt atcagaatgt tgattacaaa ccccatcaag atgattactc
      601 ttcagctgaa aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga ctttggcgtt
      661 gatttccagc cgtcctgagg ggcgttgcgt tgctgcctgc ggtgatttcg ggctagttat
      721 gaaagcatat tttgacaaga tggaatcaaa tggcatcagt gttatggcag ccatattact
      781 ggtggataac catgctttga cggtccggct aagaataaag aacacaactg aaggatgtac
      841 ccattacgtg gtttcggttt atgatcctaa tgtaactaac gataaaataa gaattatgag
      901 cgaaagcaaa gaggatatta aacactattc tctgatggat tttatgaatg tagattatag
      961 cctcctgaaa tggtcaaatg atcatgttat taaccaatct gttgcaataa ttccagcact
     1021 tccgaaagaa cagctattga tgttaaaagg aactgtggat gaaataaccc ctccattatc
     1081 accagcaacg atgaatttgc taatggcaat tggtcagaat caccaactta cgcaactgat
     1141 gattcagctc cagaaaatgc cagaactaca tagaacagaa atgttgactg cctataatag
     1201 tattaacctc cccggtttat atttggctat aaattatggc aatgcggata tcgttgagac
     1261 tattttcaat tcattgtcag aaacaggata tgaagggtta ctctcgaaaa aaaatctcat
     1321 gcatattctg gaggcaaaag ataaaaatgg tttttctgga ttatttttag cgatatcacg
     1381 taaggataaa aatgttgtaa cctcgattct gaacgtctta cctaaactgg ccgcaacaca
     1441 tcatttagat aatgaacagg tgtataaatt cctgagtgcc aaaaatagaa cgtccagcca
     1501 tgttttatac catgttatgg cgaatggtga tgccgacatg ctgaaaattt ttttggtcgc
     1561 gttacctttg ttaattcgca catgtcattt gactaaagaa caggtactcg atctcctgaa
     1621 ggcaaaggat ttttatggtt gcccaagact atacctggcg atgcaaaatg gacatagcga
     1681 tatcgtgaaa gttattctcg aagcattgcc ctgtctggcc caggaaatta acatttcagc
     1741 ttccgatatt gtagatcttc tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat
     1801 ggccatgcag cgcggacata tgaacgttat taatactatt tttaacgcat tacccactct
     1861 ttttaatacg tttaaattcg ataaaaaaat atgaagcccc tcctcctggc aaataattct
     1921 aatgaatatc ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg
     1981 gtttatcttg ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg
     2041 gatttttggc aacacaaagc gccacaaaaa tactctgcct ttgagttggc ttttgaattt
     2101 ggtcaccggg ttattgctga attaatcctt aatacattaa ataagatggc tgaaagcttt
     2161 ggctttacgg ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa
     2221 aaagcatctc cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt
     2281 ccacatgccc ttcacgtatg cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt
     2341 cggcgtgcat ctcttcaaac agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa
     2401 actcttccgg gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt
     2461 cttccggata gcgcggcggc gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt
     2521 catcgtaatc ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca
     2581 tgtcgccagg gaaaatgtta gcagcggcaa gctggcgaat agcgttaccg tattcttcaa
     2641 tggcgtcgcg caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc
     2701 gccgctcgat tatagagatg gcgaattaca atctgctcac caagatcggt gattttttcc
     2761 gctgcttcct ggagcagtaa ttccattaat accggggaaa tatgccgctt ctccagcaca
     2821 aagttttcaa actcctgggt gtccgccatt cggcccacac ggtcgtgctc tttcaccagt
     2881 tgatagcagg cacgaacgtg agcggcagac atctctttct gcggcgcgaa cttgtctttg
     2941 atgactttga atacccgatc aaagcccggc agcgtaaaca ccaacatcac cattccacga
     3001 atacccggcg cttcaatgaa ctgctcatta cagccctgta gataaacgag atattcgcgg
     3061 tagctttcgg ttttggcgtg cttctggcag ccgatagcca tatacaattc agcagtggtt
     3121 ttacctggca gaatttcccg tagccactcg accagcgctg cgggcagcgg tgcataaacc
     3181 ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag
     3241 gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg
     3301 ccggaaggcg tgatcagttt gcccaccagc caggcggctt tattgcggta aaacagttcg
     3361 ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg
     3421 cgaatgatgt aatgaatgtc acggctttta ttctgccagc gcaggcgcag cggcaggtcg
     3481 ctgataacgc gcatcagtag agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc
     3541 gggcggggaa tggtacgaaa gcggcgctct ggctgagagc taaagataaa aagccgctcg
     3601 ggagtaagcg agcggtggtc aaataaccga cagtacacgg agttaaaaaa gctctccgca
     3661 atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa
     3721 aatgccgcgt ccgtactttg gccgttagta atgcagcgca gttgctccac gaccagacca
     3781 acgtgatgat cgtaaagatg gatacggttt ttcatcgcct gctggacagc atgccagtcg
     3841 gcctgttcga aacgctgctg cgccccggaa gtcacttcga ggaatcgacc atactgagcg
     3901 tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca ggccacgcgg catttagcgc
     3961 cctcatcagg agcagagaat tgccggatgc ggcgtaaacg ccttatccag cctacgttcg
     4021 gcaacggttg taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg
     4081 ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc aacgtttgta ggttgcttag
     4141 aactgcgatt cttcagtgga gccagtcagc gcggtgactg aagacgtgcc gccctgaata
     4201 atagtcgtca ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta
     4261 tagccatctt tcgcggcggc aaattccggc tgctgcactt tctcaacata gtgcttcata
     4321 ccttcgccct gcgcataagc gtttgccagg tcgaacatgt taaaccacat gctgtggata
     4381 cctgctaggg tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg
     4441 gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc
     4501 agcagtttgc ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga
     4561 tccggcgtgg aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg
     4621 ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgccg
     4681 gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga tcagatccgc cgcatcagca
     4741 tcggtacgtg caaccagcag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg
     4801 accagtttct gaatagcttc ctgagttggc actaaaactt tgccgcccat gtgaccgcat
     4861 ttcttcactg acgccagctg atcttcgaag tgaactgccg cagcaccggc ttcaatcatc
     4921 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
     4981 atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccac ggaccattgg
     5041 atctgatcgg cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt
     5101 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
     5161 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
     5221 ccagtcagtg caccgaggtt gttgatgtag ccttttttcg actcaccgtg cagcagacgc
     5281 cacattttcg ctgcgcccag ttgcgccagc gtgcattcag gattgactga accgcgtaat
     5341 ttcaccacat cttccgcact gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac
     5401 tctttctgta attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt
     5461 atgtggtggt ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat
     5521 cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc aaaacggccc tgggagaaac
     5581 gttcttcgcc cagttcgctg gcaatgactt tcatctcttc gcccagcatc tggcggaaca
     5641 aggctttggt caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga
     5701 tcgaggtacg ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc
     5761 agccgttgcc ggagatccac gcttcgatgt actgcacagc cacgcgaatg ttagcgcgca
     5821 taccttcttc ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg
     5881 cgtcttgttc gcgcaatact tcaagctgat ttttacggga gccgagaatg tcgttgaata
     5941 ccgccattgc cgtgtccgca aggcctgggt gagcgatcca tgtgccatcg tgaccgttat
     6001 tggcttccag cgctttatcc gcttttactt tgttgagcac ctggttatta cgctcttcat
     6061 ctttgctcgg aataaacgcg gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg
     6121 ttttaatcag caggcgtgag taagcattca ggaatggttt atccatcgtc acggcctgtc
     6181 tgtctggcag gacacgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat
     6241 cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag cgcgtgaagg atttcgtcca
     6301 tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca
     6361 gattaaagcg atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgctagg
     6421 actgggtttt cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt
     6481 tgtggaagaa atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg
     6541 tgacatgttt ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg
     6601 gcttgagctg ataaattttg cctgcttcat tggtatagct gatggtaccg ttaaccgcat
     6661 cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga
     6721 aatcggccat aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcta
     6781 ccgggccagt tatctctacg cggcggtctt ctaagtccgc aggaatcccg cgaattttcc
     6841 aatcagcatc gcgaatggaa gccgtttccg aaataaaatc aggcaacgtt ccgttatcaa
     6901 tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg
     6961 tcaccagctc agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc
     7021 catacggcct tgtgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc
     7081 tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa ggaacgttgt gcgcttttcg
     7141 ttcagcacaa ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat
     7201 taaaaatact tgataactta ttgataacaa tatgaataaa atttattcac ttgctgaggt
     7261 gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa
     7321 cgatcgacta tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc
     7381 gtgatctgat agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc
     7441 cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga aatagttata cggtacatcc
     7501 gggtctagtc cggcttccac atcgcgtaaa tattcctgcg ccagcgtttg cgcatcatat
     7561 tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc
     7621 ccttcttccg tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga
     7681 aagtcagcat agcgcgaatg cggtgccagg aatgaatcat caaagccacg cgtcagaagc
     7741 gcatgaggat ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc
     7801 ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc
     7861 gaggtgacgt gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca
     7921 tcattaaact ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt
     7981 tcctgaatat cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc
     8041 gattcacggg aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc
     8101 aggcgcagaa actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc
     8161 ttaagtggac gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct
     8221 tcacgcaaga aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta
     8281 cctcactaca tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt
     8341 gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa
     8401 tataagaatg acgaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg
     8461 aaggggagga actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat
     8521 cagcagagta tcgggccgag ctggaagagc tggtgcgttc ctttctgccg gaagcaccgt
     8581 tgtgggtcgc ggttaatgaa cgggagcagc cggttgggtt tatgttgtta agtgggcagc
     8641 atatggatgc gctgtttatc gatcctgatg tgtgcggctg cggcgtgggt cggatgctgg
     8701 tggagcatgc gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc
     8761 aggcggttgg gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg
     8821 atttggggaa accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcctctg
     8881 ccgtgcctgc ccaagctgga gaagacg
//
LOCUS       DAZPFP010000154         8760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPFP010000154 DAZPFP010000000
VERSION     DAZPFP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.155"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUR4_003223"
     CDS             <1..439
                     /locus_tag="ACHUR4_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1456693.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUR4_003224"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUR4_003224"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1456694.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUR4_003225"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUR4_003225"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1456695.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUR4_003226"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUR4_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1456696.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHUR4_003227"
     CDS             complement(4441..5148)
                     /locus_tag="ACHUR4_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR1456697.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUR4_003228"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUR4_003228"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1456698.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHUR4_003229"
     CDS             complement(6006..6209)
                     /locus_tag="ACHUR4_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1456699.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHUR4_003230"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHUR4_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8760
                     /locus_tag="ACHUR4_003231"
     CDS             7873..>8760
                     /locus_tag="ACHUR4_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1456700.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2389 c         2251 g         2088 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DAZPFP010000155         8723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPFP010000155 DAZPFP010000000
VERSION     DAZPFP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.156"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUR4_003232"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUR4_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1456701.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHUR4_003233"
     CDS             696..914
                     /locus_tag="ACHUR4_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1456702.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHUR4_003234"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHUR4_003234"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1456703.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUR4_003235"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUR4_003235"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1456704.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUR4_003236"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUR4_003236"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1456705.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUR4_003237"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUR4_003237"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1456706.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUR4_003238"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUR4_003238"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1456707.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUR4_003239"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUR4_003239"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1456708.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUR4_003240"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUR4_003240"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1456709.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2005 a         2163 c         2465 g         2090 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat gatgcaaaag
     8701 taacacattt aatttattaa tta
//
LOCUS       DAZPFP010000156         8679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPFP010000156 DAZPFP010000000
VERSION     DAZPFP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.157"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(42..134)
                     /locus_tag="ACHUR4_003241"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHUR4_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHUR4_003242"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHUR4_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1456710.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHUR4_003243"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHUR4_003243"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1456711.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHUR4_003244"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHUR4_003244"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1456712.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHUR4_003245"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHUR4_003245"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1456713.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHUR4_003246"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHUR4_003246"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1456714.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHUR4_003247"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHUR4_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1456715.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="ACHUR4_003248"
     CDS             complement(8258..>8679)
                     /locus_tag="ACHUR4_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1456716.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1975 a         2241 c         2235 g         2228 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttcccctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAZPFP010000157         8654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPFP010000157 DAZPFP010000000
VERSION     DAZPFP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.158"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..542
                     /locus_tag="ACHUR4_003249"
     CDS             <1..542
                     /locus_tag="ACHUR4_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR1456717.1"
                     /translation="NFMRQHRFSKVNFDTQQMIHFINRFNMNKGLVDKNNNFFIQLID
                     QALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSS
                     VDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYA
                     LFPIFTASWRATRIKNKGI"
     gene            765..1262
                     /gene="ubiC"
                     /locus_tag="ACHUR4_003250"
     CDS             765..1262
                     /gene="ubiC"
                     /locus_tag="ACHUR4_003250"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1456718.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1275..2147
                     /gene="ubiA"
                     /locus_tag="ACHUR4_003251"
     CDS             1275..2147
                     /gene="ubiA"
                     /locus_tag="ACHUR4_003251"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1456719.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2302..4725)
                     /gene="plsB"
                     /locus_tag="ACHUR4_003252"
     CDS             complement(2302..4725)
                     /gene="plsB"
                     /locus_tag="ACHUR4_003252"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1456720.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4896..5264
                     /gene="dgkA"
                     /locus_tag="ACHUR4_003253"
     CDS             4896..5264
                     /gene="dgkA"
                     /locus_tag="ACHUR4_003253"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1456721.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5374..5982
                     /gene="lexA"
                     /locus_tag="ACHUR4_003254"
     CDS             5374..5982
                     /gene="lexA"
                     /locus_tag="ACHUR4_003254"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1456722.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6055..7380
                     /gene="dinF"
                     /locus_tag="ACHUR4_003255"
     CDS             6055..7380
                     /gene="dinF"
                     /locus_tag="ACHUR4_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1456723.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7496..7705
                     /locus_tag="ACHUR4_003256"
     CDS             7496..7705
                     /locus_tag="ACHUR4_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1456724.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7747..8262)
                     /gene="zur"
                     /locus_tag="ACHUR4_003257"
     CDS             complement(7747..8262)
                     /gene="zur"
                     /locus_tag="ACHUR4_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1456725.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8580..>8654
                     /locus_tag="ACHUR4_003258"
     CDS             8580..>8654
                     /locus_tag="ACHUR4_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456726.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKD"
BASE COUNT         2025 a         2063 c         2315 g         2251 t
ORIGIN      
        1 ataattttat gcgtcaacat cgattcagta aggtgaattt cgatacccaa cagatgatcc
       61 attttattaa cagatttaat atgaataaag ggctggttga taaaaataac aattttttta
      121 tccaacttat cgatcaggcc ttacgatcaa cggatgatat gatcaaagca aatgcctggt
      181 atctttataa agagtggatt cgtagtgatg atgtttcacc tatatttata gaaactgaag
      241 aaaagttaag aacctttaac actaataaat taacacgaaa cgataatata tttatcctgt
      301 tctcctctgt cgatgatggg cctgttatgg tggtaagctc ccagcgctta catgatatgt
      361 tgaatcctac aaaagatacc aattggaatt ccacgtgtat ctataaatcc agacataaga
      421 tgttgcctat taatcttact caggaaacac ttttcagctc caaatctcat ggtaaatatg
      481 cgcttttccc aatttttact gcgagttggc gagctactcg tataaagaat aaaggtattt
      541 aagtaaagga aaacatcacc gttcctagca tcatgggcgg tgatgcccct atggttgccc
      601 tcgccagcac gggcatcggc aaagcgtaag gttcaacagc gttttaccac ttcatgcgat
      661 tgttgcgttt ttgttgcgca ttagatcact taatttgctt tacatctccc gtaaacactt
      721 ttctgcgata caatgccttt acgttatgta acggagagtt cggcatgtca caccccgcgt
      781 taacgcaact gcgtgcgctg cgctatttta aagagatccc tgccctggat ccgcaactgc
      841 tcgactggct gttgctggag gattccatga caaaacgttt tgaacagcag ggaaaaacgg
      901 tgagtgtgac gatgatccgc gaggggtttg tcgagcagaa tgaaatcccc gaagaactgc
      961 cgctgctgcc gaaagagtct cgttactggt tacgtgaaat tttgttatgt gccgatggtg
     1021 aaccgtggct tgccggtcgt accgtcgttc ctgtatcaac attaagcggg ccggaactag
     1081 cgttacaaaa attgggtaaa acgccgttag gacgctatct gttcacatca tcgacactaa
     1141 cccgggattt tatcgagata ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc
     1201 gattaagcgg taaaccgctg ttgctaacag aactgttttt accggcgtca ccgttgtact
     1261 aagaggaaaa aaatatggag tggagtctga cgcagaataa gctgctggcg tttcatcgct
     1321 taatgcgtac ggataagcca attggcgcgt tactgctgct ctggccaaca ttatgggcgt
     1381 tgtgggtggc gacaccgggc gttcctcagc tctggatcat ggcggtgttt gtcgcgggtg
     1441 tctggctgat gcgcgctgct ggttgcgtgg tgaatgatta tgctgatcgc aagtttgatg
     1501 gtcatgttaa gcgcacggcg aaccgaccac ttcccagcgg cgcggtaaca gagaaagagg
     1561 cgcgcgcgct gtttgtagtg ctggtactga tttcgttttt actggttctg acgctgaata
     1621 cgatgaccat tctgttgtcg attgccgctc tggcattggc gtgggtgtac ccgtttatga
     1681 agcggtatac ccatctcccg caagtggtgc tgggcgcggc gtttggctgg tcgattccaa
     1741 tggcttttgc cgctgtgagt gagtcggtgc cattgagttg ctggttaatg ttcctcgcca
     1801 atattctctg ggcggtggct tacgacacgc agtatgcgat ggttgaccgc gatgatgatg
     1861 tgaagattgg cattaaatcc acggcaatcc tgttcggcca atacgataaa ttgattattg
     1921 gtattttgca gattggcgta ctggcactga tggcgatcat cggtgagtta aatggcttag
     1981 gctggggata ttactggtca attgtggtgg ctggcgcgct gtttgtttat caacaaaaac
     2041 tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat aactatgttg
     2101 gtctggtact gtttttagga ctggcaatga gttactggca tttctgatga tgtaaaaaag
     2161 ccggatgatc atccggcttt cttctgggtt gcctgatgcg ttgcgcttct caggcctacc
     2221 cggtgaatcg caatttattg aatttgcagg tcatggtagg ccggataagg cgttttcgcc
     2281 gcatccggca attctctctg attacccttc gccctgcgtc gcactctcaa tcgtcaaacg
     2341 cacgtctgat gtaatcaact ccgccagcaa ctgataaacc ttcatcgttt ctgctggttc
     2401 ggcatcgccg ctatcgctga tatacccttc atcacgcagc gtcagcacca gagaactgaa
     2461 caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc
     2521 aacggtgcgg ctctctttct ccagcgtacc gcggttgatc gacgggttgg cactcaacaa
     2581 ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag
     2641 ggtgcgagaa tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg
     2701 acgttgcatc tcatttgcca gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg
     2761 caggaacagc tccgctttca gcattgggta aagcacattg acgtgctcca tcaatacgtc
     2821 gcgggagatg tggcgatgct gggtgacaat tgccgccatc agcgaaggca gcaccaacat
     2881 atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct tgctcgcgtg gcagaatgat
     2941 gatgtcgccg atagtgtctt tctcgacttc aaacttgttc atttgcagcg cgtgatcgat
     3001 aagctcgctg gcgctggctg aaggaacggt agagtccgtg gagtaaggta cgttgcgcat
     3061 cagatccagg tagcagttga gttgctcggt taactgctcg cgggtgagtg agcgctgacg
     3121 tgatgccagt agcgcagtac agcacaggtt catggcgttt gccgcgcctg cgttgttaat
     3181 gcgtaccatc agatcggcag caatattatt gaccgtcggc gttaaccagg ccggacgcac
     3241 cgcttcgatg ggatcgatag attcacgcca gtctggtacg tgctggttaa ggtaggtcat
     3301 caacggcatt ggttcaccga agttgacgta accctgaccg agattacgca gcttgcttaa
     3361 accgcgcagc atctgcggca ggctctcttt ctctttcgtc gcgccgcgca gttctttggc
     3421 gtaagtaccc acttccatga cgtgctcata accgatatag atcggaatca gcgtaatcgg
     3481 acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc
     3541 cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag tactcgacgg aataaccacg
     3601 gctgaacagt tcgccgagat actcacggaa aacggtggaa taaagtttat tgcctttaaa
     3661 agtacggcga ataaagaacg cccccagacg gcggaaaatc ggcccggccg gccagaagtt
     3721 caggttgatc ccggcggcga tatgcggcgg caccagtccc tggtggtaca gcacgtaaga
     3781 aagtagcagg tagtccatgt gactgcggtg gcaaggcaca tacaccagct catgaccgtc
     3841 gtgggccagc tggcgaacgc gctcggcgtt atggacgttg atgccctgat aaaatcggtt
     3901 ccaggtgaag cccagaatgc ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc
     3961 aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga tttttttgct
     4021 gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag gcgagcagct tattaaacag
     4081 atcctgacga gcaggcagac gtgggcctac ggcagccaga cgttgacggg caaagtgcat
     4141 acgcgccacg cgcgccagtt tctgagcgat agttttatcc gtgccgtgtt catccgccat
     4201 acggcgcagc gacactgacg gcgagaaacg cacaaaactg tcgcgaccga gccacagtac
     4261 agcgaaaaat ttctgtacgc cgttaagcat acgcagcggc gggttcactt cgcctttttc
     4321 acgccccggc gcgcgaccaa acatcaccga tactggcacc atctgcacat ccagatttgg
     4381 gttgctacgg tgcaaatcga gatagtcgtg gaaaagctta atagactctt ctttcggcgt
     4441 gtaataggtg aatacacgcg gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc
     4501 gtcgatttcc agcggctcta acgggtcagg caaatcatgt gccagacact gggcgcgcaa
     4561 cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac ataattggac gagaggtatc
     4621 cagccccagt tccggggcag gatctgccgg aatagacttg ctttttacca ggatgcttaa
     4681 tggtaaattc agtaatttgt agtaaattcg tggccagccg gacataaacg atgtaaagcc
     4741 tctggttaat aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa attctgtggt
     4801 atccgctcat gtttcgcgcg gcgttacgca aacccgaatc atcggattta acggtacact
     4861 gatattgacg ctcataatgt aaaaaggttc tttcaatggc caataataca actggattca
     4921 cccgaattat caaagctgct ggctattcct ggaaaggttt acgcgctgca tggatcaacg
     4981 aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc
     5041 tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg
     5101 tggaaatcct caatagcgcc atcgaagcag tggttgaccg aattggctct gaataccatg
     5161 agctttccgg acgcgcaaaa gatatgggat ccgccgcggt gctgattgcc attatcgtcg
     5221 ccgtgattac ctggtgcatc ctgttatggt cgcattttgg ataacccttc cagatttcga
     5281 taaatctctg gtttattgtg cagtttatgg ttccaaaatc gccttttgct gtatatactc
     5341 acagtataac tgtatataca cccagggggc ggaatgaaag cgttaacggc caggcaacaa
     5401 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg
     5461 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg
     5521 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg
     5581 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accgcttctg
     5641 gcgcaacagc atattgaagg tcattatcag gtcgatcctt ccttgttcaa gccgaatgct
     5701 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggcgac
     5761 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt
     5821 gatgacgaag ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga actgttgcca
     5881 gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt caccattgaa
     5941 gggctggcgg ttggggttat tcgcaacggc gactggctgt aacatatctc tgagaccgcg
     6001 atgccgcttg gcgtcgcggt ttgtttttca tctctcttca tcaggcttgt ctgcatggca
     6061 ttcctcactt catctgataa agcactctgg catctcgcct tacccatgat tttctccaat
     6121 atcaccgttc cgttgctggg actggtcgat acggcggtaa ttggtcatct tgatagtccg
     6181 gtttatttgg gcggcgtggc ggttggtgca acggcgacca gctttctctt tatgctgttg
     6241 ctgtttttac gcatgagcac caccgggctg actgcgcagg cttatggtgc caaaaatcct
     6301 caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg cgttgggggc cggggcgtta
     6361 attgcgctgt tgcgtacgcc gattatcgat ctggcgctgc atattgttgg cggtagtgaa
     6421 gcggtcctgg aacaggcgcg gcgctttctt gaaatccgct ggttaagcgc tccggcgtcg
     6481 ctggcgaatc tggtattact cggttggtta ctcggcgtgc aatatgcccg tgcgccagta
     6541 attttgttag tggtcggcaa tatcctcaac attgtgctgg atgtctggct ggtgatgggg
     6601 ctgcatatga acgtgcaggg cgcggcgctg gcgacggtta ttgcggaata tgcaacattg
     6661 ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg
     6721 ctgaaaactg cctggcgagg aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg
     6781 ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca ccgtacttgg tgcgcgactg
     6841 gggagtgaca ttatcgctgt taacgcggtt ctgatgacgc tactcacctt taccgcctat
     6901 gcgcttgatg gttttgccta cgcggttgaa gcgcactccg gtcaggcata cggtgcgcgc
     6961 gacggtagcc agttgctgga tgtatggcgg gcagcgtgcc gccagtcggg gattgtagcg
     7021 ttactatttt cggtggttta tttgctggca ggggaacaca tcattgcgtt gctgacgtcg
     7081 ttaacccaga ttcagcagct ggctgaccgc tatcttatct ggcaggtgat tttgccgttg
     7141 gttggcgtct ggtgttatct gctggacggc atgtttatag gcgcaacgcg tgccgccgaa
     7201 atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc tgacgctcct tacgctgccg
     7261 tggctgggta atcatggttt gtggctggca ttaaccgtct ttctggcgtt gcgcgggctt
     7321 tctctggcgg ctatctggcg gcgtcactgg cgcaacggta cctggtttgc cgcaacgtga
     7381 cggttaaaaa ttctgaataa attatcctaa gccaaattgc tgactacact taatctcacg
     7441 ttcagaagaa aagtgaacgt actctcattc acaacctaac gatgaggtct tgattatgaa
     7501 taaagatgaa gccggcggta actggaaaca gtttaaaggt aaagtgaaag agcaatgggg
     7561 caaactgacc gatgatgata tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa
     7621 aatccaggaa cgttatggtt atcagaaaga tcaggcagaa aaagaggtcg tggattggga
     7681 aacccgcaat gaatatcgct ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac
     7741 gccctcttaa cgcggtttct ttttcacctg cacagagtga tcatgctggc actgttcagg
     7801 atgacgacac gcttccactt ccacgcatgc cgcacagagc ccatgtgctt caatcacatt
     7861 atgccgcagg gcaaacccca tttttgccgc cagcgtatgc ataatgtctt ccacgccttc
     7921 tgcacactct tctttcaccg cgccgcagcg atcgcaaata aacatggctg acgtatgcgt
     7981 gggctggtcg aacagatgac agagcacata actgttggtg gattccacct tatgcacaaa
     8041 accttgctca agcagaaaat ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc
     8101 agcttcgcgc agtaaatcaa gcagatcata agcgctgata gcgccatcct gcagactcat
     8161 caggcgcaac acttccaggc gctgtggggt caggcgcaca ttacgctgcg cgcagatttt
     8221 ttcagcctgc gctaataact cctgcgttgt ggtcttttcc atcgggcacc tcaaagtggg
     8281 ggttaagaat gtccttactt taccatgttc caggaaaaac accgagaacc cttattgttg
     8341 ccgtaatgtt gattttctgt tttgtaggta aggtgttgtg ttgccttatc gtaccattat
     8401 caacacgata ataattaata attcattttt aaataaattg cttcaagtta actcgctatt
     8461 tatttaactg ggtttaaacg catacctgta gttcagaaat tatgagcgat ttttgatagt
     8521 gaccagatgg caaaagaatt ataaggagga cacggtacga caaaaaaggt agatgaatca
     8581 tgttgaagat aatacccggt gcaactggat actttaataa aacactcaat tcaaatcaat
     8641 tcgaaaataa agat
//
LOCUS       DAZPFP010000158         8648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPFP010000158 DAZPFP010000000
VERSION     DAZPFP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.159"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..551)
                     /gene="gloB"
                     /locus_tag="ACHUR4_003259"
     CDS             complement(<1..551)
                     /gene="gloB"
                     /locus_tag="ACHUR4_003259"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1456727.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLS"
     gene            585..1307
                     /locus_tag="ACHUR4_003260"
     CDS             585..1307
                     /locus_tag="ACHUR4_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1456728.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(1304..1771)
                     /gene="rnhA"
                     /locus_tag="ACHUR4_003261"
     CDS             complement(1304..1771)
                     /gene="rnhA"
                     /locus_tag="ACHUR4_003261"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1456729.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            1836..2567
                     /gene="dnaQ"
                     /locus_tag="ACHUR4_003262"
     CDS             1836..2567
                     /gene="dnaQ"
                     /locus_tag="ACHUR4_003262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1456730.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2700..2776
                     /locus_tag="ACHUR4_003263"
     tRNA            2700..2776
                     /locus_tag="ACHUR4_003263"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2734..2736,aa:Asp,seq:gtc)
     gene            complement(2907..3953)
                     /locus_tag="ACHUR4_003264"
     CDS             complement(2907..3953)
                     /locus_tag="ACHUR4_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR1456731.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(3967..4526)
                     /gene="tssG"
                     /locus_tag="ACHUR4_003265"
                     /pseudo
     CDS             complement(3967..4526)
                     /gene="tssG"
                     /locus_tag="ACHUR4_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(4523..6406)
                     /gene="tssF"
                     /locus_tag="ACHUR4_003266"
     CDS             complement(4523..6406)
                     /gene="tssF"
                     /locus_tag="ACHUR4_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1456732.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(6422..6916)
                     /gene="tssE"
                     /locus_tag="ACHUR4_003267"
     CDS             complement(6422..6916)
                     /gene="tssE"
                     /locus_tag="ACHUR4_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1456733.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(6913..7743)
                     /locus_tag="ACHUR4_003268"
                     /pseudo
     CDS             complement(6913..7743)
                     /locus_tag="ACHUR4_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(7730..8644)
                     /gene="tagK"
                     /locus_tag="ACHUR4_003269"
                     /pseudo
     CDS             complement(7730..8644)
                     /gene="tagK"
                     /locus_tag="ACHUR4_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         1933 a         2282 c         2414 g         2019 t
ORIGIN      
        1 gacaaatcgt gcggaaaaat actcaaagca aacttcatat ttgataacgt atattcatga
       61 gcacaacata ccaatgtatc gtcaggtaac gcacttagct ttttaagtga ttgatacatt
      121 tgtaaagctg tcccttcaaa caaccgacca cacccaccag aaaacagcgt gtcgccgcaa
      181 aatagataag gtttactgaa gtaacagata tgtcctaaag tgtgacccgg tgtggtaatt
      241 acactaaatt catgccctaa aacgaaggca gtttcgccat ctttgactac ctgtgttgtt
      301 cccttatctt gtgtctcttg tggaccatac actacaattt gcggaaactt ttccaccagt
      361 tcttttacgc cgccaacgtg atcgtggtga tggtgggtta gaaatatggc ctccggttgc
      421 cagttattgg cggtaatggc gtttaatact ggctctgcgt ctccgggatc gacaatcagg
      481 cagcgacctg cctcatcatt caaaacccag atgtaattgt catcaaaggc gggaatactg
      541 ttaagattca tagattacct ctcagtgtaa aacggaaggt tgtgatgaaa ccggcaagag
      601 tccctcaaac tgtcgtggct cctgattgct ggggcgattt gccctgggga gagctttatc
      661 gcaaggcgct ggagcgccag ctcaacccgt ggttcactaa aatgtatggt tttcatctgc
      721 ttaagattgg caatttaagc gcagaaatca attgcgaagc gtgcgcggtt tctcatcaag
      781 tgaatgtttc tgcgcaagga atgcccgtcc aggtgcaggc ggatccactt catcttcctt
      841 ttgccgataa atccgttgat atttgtctac tggcacatac cttgccgtgg tgcaccgatc
      901 cgcatcgttt attacgtgaa gccgatcggg tactgattga tgatggctgg ctggtgatta
      961 gtggcttcaa ccccatcagt ttaatgggat tacgcaaact tgtgccggta ttgcgcaaaa
     1021 cctcgcccta taatagccgg atgtttactc tgatgcggca actggactgg ctctctttgt
     1081 tgaattttga agtgctgcac gccagccgtt tccacgttct cccgtggaac aaacacggag
     1141 gaaaactatt gaatgcgcat attcctgcgc ttggttgctt acaacttatt gttgcccgga
     1201 aacggactat tcctttaacg ctaaatccga tgaaacagag taaaaacaag ccacgaattc
     1261 gccaggcggt tggagccacc cggcaatgtc gtaaaccaca ggcttaaact tcgacttggt
     1321 agcccgtatc ttccagtgtg ggattcatcg ccgcggcacg agccagttca tcacagcgtt
     1381 cgttttccgg gtgtccggca tggcctttaa cccattccca tttgatttga tgctgcccca
     1441 atgcagcatc aagacgttgc cagagatcga cattttttac tggttttttg tctgctgttt
     1501 tccagccacg ttttttccag ttatggatcc actgggtgat accctggcgg acatactggc
     1561 tgtcggtact caaaatgact tcgcaatgtt cttttaacgc ctccagcgcg acaatagcgg
     1621 ccatcaactc catacggttg ttggtggtgc gggtgtagcc tgcgctaaag gttttctcgc
     1681 gtccgcgata gcgtaaaata gcgccgtaac ccccaggtcc aggattaccc agacacgaac
     1741 catcggtgaa aatttctacc tgtttaagca tctctggtag acttcctgta attgaatcga
     1801 actgtaaaac gacaagtctg acataaatga ccgctatgag cactgcaatt acacgccaga
     1861 tcgttctcga taccgaaacc accggtatga accagattgg tgcacactat gaaggccaca
     1921 agatcattga gattggtgct gttgaagtag tgaaccgtcg cctgacgggc aataacttcc
     1981 atgtttatct caaacccgat cggctggtgg atccggaagc ctttggcgta catggtattg
     2041 ctgatgaatt tttgctcgat aagcccacgt ttgccgaagt agccgatgag tttatggact
     2101 atatccgcgg cgcggagttg gtgatccata acgcagcgtt cgatataggc tttatggact
     2161 acgagttttc gttgcttaag cgcgatattc cgaagaccaa taccttctgt aaggtcaccg
     2221 atagccttgc ggtggcgagg aaaatgtttc ccggtaaacg caacagcctc gatgcgctat
     2281 gtgcccgcta cgaaatagat aacagtaaac gaacgctgca cggggcatta ctcgatgccc
     2341 agatccttgc ggaagtttat ctggcgatga ccggtggtca aacgtcgatg gcttttgcga
     2401 tggaaggaga gacacagcag caacaaggtg aagcaacaat tcagcgcatt gtacgtcagg
     2461 caagtaagtt acgcgttgtt tttgcgacag atgaagagct ggcagcacat gaagcccgtc
     2521 tcgatctggt gcagaaaaaa ggcggaagct gcctctggcg ggcataaata catgtaaaag
     2581 gcgctaaaaa tagcgacttg ggcgattttt gcagcaaacg attcaaaaga tgagaaaaat
     2641 cgttgacgaa ggtcgaggca atccgtaata ttcgcctcgt tcccaacgga acacaacgcg
     2701 gagcggtagt tcagtcggtt agaatacctg cctgtcacgc agggggtcgc gggttcgagt
     2761 cccgtccgtt ccgccaatat tcagaaagcc cgaatcagta atgattcggg ctttcgtcgt
     2821 tttggtgaag tgagaatttt tcactccgaa gtaaaacgga tatgatttaa aaaatggcca
     2881 gacattataa attctggcca cttctgttac tccagcgcat cccatttctc cagcagttct
     2941 actggaattt catttattac ctccgagacg cgttttccga ccaggcgttc tgcctggcgc
     3001 agcaaaggaa tggtcgggct gctcggttcg ctgtgctcaa accagcgacg gatgacacgc
     3061 aggcgttcca gcgcgtcgtt tcggtcgcgg atctgccggg gttctgcgcc ggtcatcggt
     3121 gatatttcct ccgcgatatg caccggtagc gcaatctgct ctgcctgcat ctgttcagta
     3181 tgaacgatgg tggcgttttc cggctgctca gcctgtggtt gtggcaggat ctccggcgtg
     3241 gtgctctgca ctgctcccgg taacagggcc agcagttgcg tcagacggga gaagtccggt
     3301 gcctggtcat tcagcgtttc acgcgcatgg cgttgcagcc gctcagtggt ctctgctgcc
     3361 tggcgaaagg catccagcgg cagtgtgccg cgcgcttcca ggtctgccag ttgctggcgt
     3421 accgattctg gtgccagcgc atccgccggg cgtaatgcag atagcgaacg ctccacatcc
     3481 cgcacctgaa ggcgcatggc agcattattt gacagtgtaa tgccacgaat atccgccatt
     3541 acaccttcgt ggtccagcaa cgccgccagc gcgttactgc gtgccaccgc agcatcttcg
     3601 gcggtgatat cttcagtcgc gagcagttga ggatggatgg cgtccgggta cgtggagcac
     3661 aacgtttcca gcagcgtgag cgcctccgac agtcccctgg caccggcctg ctggatacgg
     3721 cagcgcagca ggacaaccag aatgcggata tctttgctgc gcatcagcag acgatgggcg
     3781 tcccgctcaa gctccgccca gttaatgttt tccggcgtac tgacaaaatc gccatattgc
     3841 gcttcttcac gcggtgcggc gcgggtgaac agcagcaggt attccggatc gtactccaga
     3901 tccgggccgc aggggcattc cgccgaaatt gggtcagaca ggaatatttc catttaatta
     3961 tcctcagtgc gccagcgtgg tggccagacc gtccggattg ctggactcac gcaccagatg
     4021 ggccgatgcc gccagacgag cgtgatctgg caacgggctg tcagcgctct cccgcaaatc
     4081 ctgcccgctg aggctggcga cataaaaggc aaagcggtca tgttgcggtt tatccagacc
     4141 gcccccagcg gactgggcgc tgcgccatgc ctggtaaaac tgcgtcagcc agcggtgatg
     4201 aaaaagatca gaaaaacggg tcagcgtcgg ctttcagtcc ggttacgcgc cagttcggtg
     4261 tagtgcagcg gcagtggacc gttcggcccc cacagcacga ggctgtacag actgatgtgc
     4321 aggcgccctt cctgccagcc cacactggcg atttctcgtg gcgcaaaggt cagcgcgggc
     4381 gtttgtccga ggcgaaattt ttccatccgt ggttgccagg tgttgcccgc cggaacggta
     4441 cacaactgca catccacgcg gcgcatcagg ttcagaaaac cgtaacgcca ggggggggcc
     4501 tgcgcctgag tgaaatcgtt gctcatacgc ttccccgctg tcccgccctg acaggccagg
     4561 tcataatttt gccgcgctgc atgctgtgca gcgtcatctg cgagaaggta ttaatggaaa
     4621 catggcgggc gatatagtgt tccagcacca ggccgaacag atacggactg atgccggaaa
     4681 aaccttcttc atccaccgtc agttcacaac tgacgccacg cccatagacc agtagtccgg
     4741 aaccgggcag acggcgggtg accggggtcg ttttacaacc aatcaggctg cgcacctggc
     4801 gcgattgcgg actgtcatga gcaggaataa acaggttcag cagatctcgc aacgcctggc
     4861 cgccggtacg gtgatccaga tcggccagcg gcagataatt aaaagataac tggcggatca
     4921 accgccatgc catctcacgt tcagccatcg ggggctgcgg cgagcgtggc gggcggataa
     4981 gccctacgcc cgccaccggg atggctgcat ccacggttag atcgtcccgg ccattgcggg
     5041 caataagaca gggcagatcg cggttagtga ccatggccgt gatggtgatg tggcgcagat
     5101 tttccgggta cggcgcttca tactggtcaa ccagcgacag gaacacttcc gagcccgtat
     5161 agggggtacg ggtgccgtaa cggcgggcgt tttctgataa gcggcggggc tcccggcgca
     5221 gggaaaaata acgtccgtga ttgccttcat cattattgcg ggtgtggtag agcgggcgaa
     5281 aggccatctt ccgtgtggtg tcggtttcca gcccttccac ttcctggacc gagaacacct
     5341 cataatccag cggatgggtt ctgtccacca ccagatgctg ttccgtcgtg ctgtgcgtga
     5401 catcgatccg ggcggtgacg cggggaaaca ggttgataac cggggtacag aacaggctga
     5461 actgtgccgc atttgtctgg tggatcagcc agtccggcgg caggcggttc agcaggataa
     5521 caatctccgc cacgccaccg tcaattttct gtaatccggc agacaggcct gtcggggtga
     5581 aaaaatagaa gcgttccgga caggcaaaat attcatgcag caggttatga ccgtgaaaca
     5641 cgttccacgc cagcggcagc agcccctgat ccggctccag gccttcgtac ataacgggct
     5701 gttgcagatt cacatccagc gcgccgtcaa aatgccccgg aatgccagcc aggggcgcga
     5761 tggcgctggt atgtagaagt tctaacaggt gtgaggccgt acgctcctcg ccgcagagat
     5821 aaaacggcag cctgtccagt ccggcaagct gactgaacgt tagttcacca aaggtgcgca
     5881 gggtaatacg cagcgccccg gcgacgtgaa tgttggccgg caggtagcgg tgcagggcag
     5941 gcatatccgg tggcgcggcg gtcagtcgcg cctcttcgat ggcgagcggc cacagcgtta
     6001 catcctggct gctgcgaaac tggcaggcag tgctttcgcc ttccggaatg gcggagaaaa
     6061 aggctgtgtc gcgcgggacg gtaaatcctt tcgccagatc gccttcttcc gtatcgggat
     6121 gcaactgcgc cacgctcatt gagggcgtgg gtgtgacgta attggggctg accacttcca
     6181 gcaggcgctg ggtgaaacgg gggaattcag cgtcaatttt tagctgcgtg cgggcggtca
     6241 ggaagctgaa ggcctcgacc attcgctcta catacggatc ggcaatatcc gttccctgca
     6301 tgcccagccg ggcggcgact ttgggatgac gggcggcaaa ttcggctccc gtttcccgca
     6361 gatagctcag ttcgcggttg tagtattcca gtagccgtgg atccatcggt ctctctctga
     6421 attaaaaaaa cgtaatgcga ctctgctcca tatccagcgc gctacgtacc cggaatgcag
     6481 tcggatacgg ctgcgtgagg atctcgccgc gaatttcaaa ctgtaatgtg ttatggccac
     6541 tctggcggcg ctcatcctga agtgggatga tttgcagagt ggacgctttc agccggggtt
     6601 caaaccgggt gatggttcgc aaaatggccc tgcggatatc gtcccacttg tgctcataca
     6661 gaaagctgcc cgccagtggc ggcaggccgt agttaagtac ggacgctgcc gcgtacggat
     6721 aacgtgcagc atcaatatca tcttcatggc tgacggtgtt gagcagaaat gacaaatccc
     6781 ggcggatgat ctccttaatc tgaaccggac tgacgctgat accgtggtcg cgtttctggt
     6841 ttggggcgtc gtcacacagg cggtcaaaca gcgtgggcag caggtgattg cctggacgct
     6901 ggcgggaagc gctcacgcgt cgcttccttc agcggtgtta aaggtacagg tggacaaatc
     6961 cagcaggctg atatcgccgt gaccggtgag ccacaccctc tgacccagcg cctgtaccag
     7021 tgtctctccc ggaccgtcct gccagaccgt ttcccggcac agacgcaggg agtcggcagc
     7081 cttttcagag ccgctgtagc ggggaaacag ctaggcactg tgtgtatcgc cattgttcag
     7141 ggtgacgttg acgggtttcc agatcaaatc gatcagatgc gcaggccgcg gcgatttcag
     7201 cgaacagatt tgtgaaaacg gcagccagat atatgctccg cccgcgatca gttcgagtac
     7261 cggaccggta cgggagtcgc tgtcgctaat ccagtcaaat gcgccgccat tccactgccc
     7321 ggaagtgtct gttatggcct caagcgcgcg gctgcggtga gcatcagctt cctgcgcttc
     7381 accacgggcg ttacaggcga gggcggtcag cagatcctcc atccaggcgg gcggcgggag
     7441 aataacaccg ggccgctgtt cgccctgaaa gcaggcgtgg cggtatatct cacagcggat
     7501 cagctcgcca aatacctgcg cttcgcgagt ataatttgcg tccatccggg cgcatagctg
     7561 gatctgttgc agtgcccgcg accagtcgcc tgccacgcac aataactgaa acaggctgtg
     7621 gcggcgggag gcctgcgctg ggtgcatcct gacctgttgc tctgccatct gaatgccctc
     7681 caccacggaa taatcccgca gcagcgccga caaggtaatc ggtagctcgt tattgtttgt
     7741 catgatatat ctctccatgt tgtgccgggt ttatccggta atggctgtca atgccaatga
     7801 tgcgatgctc tttccgtgtc agatctggca gtacggtgtt atgcgctgtt tccggggcgt
     7861 attccgggga aagtaaatgc aggatatcag ggggagtctt ttcgacaggc cagatagttt
     7921 catcttcgcc agtggcatcc agtacattga gaatagcgtc aatgcttaac ccaccagtga
     7981 ccatatcctg caatgtctcg gcttcggtga ccaggtcacg caatgaatcc agatcctgcg
     8041 aggagggctc ttcgttaaaa ggaaaaggat cagcatggcg attaagatct tgttcgggag
     8101 ataacaacgc ctcgcggtat tcctgataaa gtcgggtcag ggcatttcct ttttccgcac
     8161 cttctccggt ggaggaggct ccatgaacag gaatgatatc gaatggattt tgtgatgtca
     8221 gatgctgctg cctgaaccaa tccagatcca gatattcggt tattgcttcc gacgtgatgg
     8281 cagtctgcga tatcgttttt actgtgctca ggttcggaga tgatggattg ccatgtacaa
     8341 ggcactatga agataacccc cattctatca ggtcgccttc attcagacgc atacggtgat
     8401 gaggttccac aatctggtcg tttaccgtac agcaatattc ggtagactga ttgataatcc
     8461 accatgcttc ttcgtggcgg gtcagagtga gggcaatctt tacatcatca ttcaccttaa
     8521 aaacaggaat ccagggagag gtaacggtga agataatttg tgttccttca gtcgcgtaaa
     8581 ttgtgttatc ggcgatcccg tgcgcctgta ttttttggag tccccacgta atatttggtt
     8641 tcattgtg
//
LOCUS       DAZPFP010000159         8609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPFP010000159 DAZPFP010000000
VERSION     DAZPFP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.160"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(343..549)
                     /locus_tag="ACHUR4_003270"
     CDS             complement(343..549)
                     /locus_tag="ACHUR4_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1456734.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            862..951
                     /gene="kdpF"
                     /locus_tag="ACHUR4_003271"
     CDS             862..951
                     /gene="kdpF"
                     /locus_tag="ACHUR4_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1456735.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            951..2624
                     /gene="kdpA"
                     /locus_tag="ACHUR4_003272"
     CDS             951..2624
                     /gene="kdpA"
                     /locus_tag="ACHUR4_003272"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1456736.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2647..4397
                     /gene="kdpB"
                     /locus_tag="ACHUR4_003273"
                     /pseudo
     CDS             2647..4397
                     /gene="kdpB"
                     /locus_tag="ACHUR4_003273"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4703..5275
                     /gene="kdpC"
                     /locus_tag="ACHUR4_003274"
     CDS             4703..5275
                     /gene="kdpC"
                     /locus_tag="ACHUR4_003274"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1456737.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5268..7948
                     /gene="kdpD"
                     /locus_tag="ACHUR4_003275"
                     /pseudo
     CDS             5268..7948
                     /gene="kdpD"
                     /locus_tag="ACHUR4_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7945..8609
                     /gene="kdpE"
                     /locus_tag="ACHUR4_003276"
                     /pseudo
     CDS             7945..8609
                     /gene="kdpE"
                     /locus_tag="ACHUR4_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1835 a         2230 c         2489 g         2055 t
ORIGIN      
        1 tgcggtagcg atactaataa caaaattcgg aatttattta gatgctggaa ttagaaaaag
       61 ggatgacggc tttctcacct taccctgaag tttcacgaaa gtgtgtaagg aagtatgcaa
      121 tctaactgga gatttctgat gatctagtcg cttagagcga caatgaaggt gaccgcatcc
      181 tgggtagccc tatgaaagga tactattgaa acgtgatgct aaaacagtga tgaaatgaat
      241 aatacgatgg aaacgtcttc catgttaatt atcatggaag acgttgatgt tgaacaccta
      301 aaccagcccc ctgatacgag gctggcttaa gcgcgcaggc aatcagtaaa aatcaccggt
      361 tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc agacgcggtg
      421 caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac
      481 gcccgctgca accgcatatg tacggcgtaa aaagataagc catgcaggat attctctgta
      541 gagttccata cttttctccc ccttttgtat ctacccggtg aatagcaccg gaaaaatgaa
      601 tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc atccgaccac
      661 ttatttttgc ttattgatgg tttatttaca ttcacactgt aattaaatta cacaaaagtt
      721 aaattaatac taaccatcag ttaaatcatg gcctttgcca tttttatact ttttttacac
      781 cccgtccgca gatttttgcg aaatctttgc agccagaatt ctactcttcc ggtatcactt
      841 ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag ccggcgtgtt gctggtgttt
      901 ttattactgg gctatttggt ttatgccctg atcaatgcgg aggcgttctg atggctgcgc
      961 aagggttctt actgattgcc acgtttttac tggtattaat ggtgctggcg cgtcctttag
     1021 gcagcgggct ggcgcggctg attaatgaca ttcctcttcc aggtacagcg ggcgttgaac
     1081 gtatactctt tcgtttacct ggcgtctctg accatgagat gaactggaag caatatcttt
     1141 gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg ttgctcggtc
     1201 agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat ctggcgctga
     1261 ataccgccgt cagctttgtc acgaatacca actggcaatc ttatagcggt gaaaccacgt
     1321 tgagctattt cagccagatg gcaggcttaa cggtacaaaa ctttctttct gccgccagcg
     1381 ggattgcggt gatttttgcc ttcatccgtg cgtttactcg ccagagcatg agcacactcg
     1441 ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctagtccct gtggcgttgt
     1501 tgatcgcact gttttttatt caacaaggtg cgcagcaaaa ctttctgccc tatcaggcag
     1561 tgaataccgt tgaaggagcg caacagctgt tacccatggg accagtagct tctcaggaag
     1621 cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg tcgcatccgt
     1681 ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc ttgatcccaa
     1741 cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccagggg cgcatgttgc
     1801 tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggttatgtgg gcagaagttc
     1861 agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg gaaggtaaag
     1921 agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct
     1981 gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggcggcatg gtgccgatgt
     2041 ggctaatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt tacggcatga
     2101 tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt acaccggaat
     2161 atctgggtaa aaaaatagac gtacgcgaga tgaaactgac tgcactggcg attctggtta
     2221 ctccaacact ggtactgatg ggcgcggcgt tggcgatgat gaccgacgcc ggacgtagcg
     2281 ccatgcttaa ccctagcccg catggtttta gcgaagtgct gtacgccgtg tcgtccgccg
     2341 ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctctccgttc tggaactgtt
     2401 tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg atggcaattg
     2461 ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg ccaacgcacg
     2521 gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca ctgaccttta
     2581 tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat tgagtgagca
     2641 ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg
     2701 aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt gatgtttatc
     2761 gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag cggtgcgatg
     2821 cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac cgtactgttc
     2881 gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa cagtcttaaa
     2941 ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg
     3001 gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga agctggcgat
     3061 attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga aagcgccatc
     3121 accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc cgttaccggc
     3181 ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc cggtgagaca
     3241 tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac accgaacgag
     3301 attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc aaccgccacg
     3361 ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact ggtggcgctg
     3421 ctggtctgtc tgatcccaac cactattggt ggcctgttgt cagcgatcgg cgtcgccggg
     3481 atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca
     3541 ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg taaccgtcag
     3601 gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa
     3661 ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa
     3721 cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt accgtttact
     3781 gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa aggttctgtc
     3841 gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga tgttgatcaa
     3901 aaagtcgatc aggttgcgcg tcaaggagcc acgctgctgg tggtggtgga aggttctcgt
     3961 gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc
     4021 cagctgcgca aatgggcatt aaaacggtga tgattaccgg cgataaccgt ctgactgccg
     4081 ccgcgatagc tgcggaagcg ggtgtcgatg attttctcgc cgaagcgaca ccggaggcca
     4141 agctggcatt gatccgtcag tatcaggcgg aaggtcgttt ggtagcgatg accggcgaca
     4201 gcaccaacga tgctacggcg ctggcgcagg cagatgtcgc ggtggcgatg aactccggca
     4261 cccaggcggc taaagaggcg ggcaatatgg tggatctcga ctctaacccg accaagttga
     4321 tcgaggtggt gcacattggc aaacagatgc tgatgacccg tggctcgctg accaccttca
     4381 gcattgccaa cgatgtggcg aaatacttcg ccattattcc ggcggcgttc gcggcaacat
     4441 atccgcagtt aaatgcgctg aacatcatgc gcctgcattc gcccgactcc gccattctca
     4501 gtgcggtcat tttcaacgcc ttgattatcg tctttttgat tcctctggcg ttaaaaggcg
     4561 tgagttataa accgcttacc gcttctgcca tgttgcgccg taacttatgg atttacggtc
     4621 tgggtgggct gctggtgccg tttattggta tcaaagtcat tgatttactg ctaaccgttt
     4681 gtagtctggt gtgaggttta ccatgagtgg attacgtccg gcattatcga catttctctt
     4741 tctgttattg attactggcg gcgtttaccc gctgctgacc accgcactgg ggcaatggtg
     4801 gtatccctgg caggccaacg gttcgttgat tcgtgaaggt gatacggtgc gcggttcggc
     4861 attaatcggg caaaatttta ccggcaacgg ctattttcat ggtcgcccgt cggcaacagc
     4921 agaaatgccc tataatccac aggcttctgg cgggagcaat ctggcagtca gtaacccgga
     4981 gctggataaa cagatagccg cacgcgttgc cgcattacgg gccgctaacc cggacgccag
     5041 cacgaacgtt ccggttgaac tggtgacggc gtcggcaagc ggactggaca ataacatcac
     5101 cccgcaagcg gcggcctggc agatcccacg catcgcaaaa gcgcgtaatc tcagcgttga
     5161 acagctcacg cagctgatcg caaaatacag ccaacaaccg ctggtgaaat atatcggcca
     5221 gccggtcgtc aacattgtcg aactcaatct ggcactggat aagcttgatg aataacgaac
     5281 ccttacgtcc cgaccccgac cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc
     5341 tgaaagtttt cttcagtgcc tgtgcaggcg tcgggaagac ctgggcgatg ctggcagaag
     5401 cccagcgact gcgggcgcaa gggctggata ttgtggttgg tgtggtagaa acccatgggc
     5461 ggaaagatac cgccgccatg cttgaagggc tggctgttct gcctctgaaa cgccaggcgt
     5521 accgtgggcg gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc
     5581 tgatcttaat ggacgaactg gcgcacagta atgcgccagg ttctcgtcat cctaaacgct
     5641 ggcaggatat cgaagaactg ctggaagctg gcattgatgt tttcacaacc gtcaacgttc
     5701 agcatctgga aagtctgaat gatgtggtca gcggcgtcac cggaattcag gtacgggaaa
     5761 ccgtgcccga tccttttttc gatgccgccg acgacgtggt gctggtggac ttgcccccgg
     5821 acgatctgcg ccagcggctg aaagaaggca aagtctatat tgccgggcag gcggagcgcg
     5881 ccattgaaca ttttttccgc aaaggcaatc tgatcgccct gcgcgaactg gcactgcgcc
     5941 gtactgccga tcgcgttgat gagcaaatgc gcgcctggcg ggggcatcct ggcgaagaga
     6001 aagtctggca cacgcgcgac gcgatccttt tatgcatcgg ccataacacc ggcagcgaaa
     6061 actggtccgc gcggcggcgc ggctgacgtc acggctgggg agcgtctggc acgcggtgta
     6121 tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc
     6181 cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc cagcggaaga
     6241 gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc tcggtcgccc
     6301 agcctcgcgc cgctggtggc gtcgggaaac atttgctgac cgactggcgc gcatcgcccc
     6361 cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc
     6421 gccggatagc cgctcattta aagacaagtg gcgggtacag attcagggat gcgtggttgc
     6481 cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg cgtttgatgc
     6541 cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg gcgctatttt atggacgctg
     6601 gccttcggtg gttgccaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc
     6661 acgcggcacg ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac
     6721 cgtcgggctg gtgatcggga accttactgc tggtgtgcgt tatcaggcgc gggtagcccg
     6781 ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg
     6841 cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg
     6901 cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg
     6961 aattacgcca tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg
     7021 cgcgggcacc gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga
     7081 gaaaacctac gggctggtgg tggtggagcc ggggaatctg cgccagttga tgatcccgga
     7141 acagcagcgc ctgctggaga cttttacgct gttagtcgcc aatgcccttg agcggctgac
     7201 gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc
     7261 cctgctggca gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc
     7321 agaaatctta acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga
     7381 gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg
     7441 aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg
     7501 cagcgcgctg caaatgccgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga
     7561 accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct
     7621 ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga
     7681 gggcgaaaat ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga
     7741 gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg
     7801 gcttgggttg gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt
     7861 caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga
     7921 acttgaagac tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct
     7981 attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa
     8041 actctgcaac gcggcttgct ggaagcggca acccgtaagc cagatttgat tattctcgat
     8101 ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcccg
     8161 gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc cgcgctggat
     8221 gccggagcgg atgattatct gagtaagccg tttggtattg gcgaattgca ggcccgtctg
     8281 cgcgtcgctt tacgccgcca ctccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8341 aattttccgg tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8401 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8461 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8521 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8581 ctcgccactt cattactgaa accggtatt
//
LOCUS       DAZPFP010000160         8529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPFP010000160 DAZPFP010000000
VERSION     DAZPFP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.161"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="csgC"
                     /locus_tag="ACHUR4_003277"
     CDS             <1..190
                     /gene="csgC"
                     /locus_tag="ACHUR4_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1456738.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            311..622
                     /gene="ymdA"
                     /locus_tag="ACHUR4_003278"
     CDS             311..622
                     /gene="ymdA"
                     /locus_tag="ACHUR4_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1456739.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            717..1250
                     /gene="ymdB"
                     /locus_tag="ACHUR4_003279"
     CDS             717..1250
                     /gene="ymdB"
                     /locus_tag="ACHUR4_003279"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1456740.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1192..2673
                     /gene="clsC"
                     /locus_tag="ACHUR4_003280"
     CDS             1192..2673
                     /gene="clsC"
                     /locus_tag="ACHUR4_003280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1456741.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2681..3838)
                     /gene="mdoC"
                     /locus_tag="ACHUR4_003281"
     CDS             complement(2681..3838)
                     /gene="mdoC"
                     /locus_tag="ACHUR4_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1456742.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4232..5767
                     /gene="mdoG"
                     /locus_tag="ACHUR4_003282"
     CDS             4232..5767
                     /gene="mdoG"
                     /locus_tag="ACHUR4_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1456743.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5760..8303
                     /gene="mdoH"
                     /locus_tag="ACHUR4_003283"
     CDS             5760..8303
                     /gene="mdoH"
                     /locus_tag="ACHUR4_003283"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1456744.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2151 a         2025 c         2222 g         2131 t
ORIGIN      
        1 aggcagttca gggcaaagtc agacgaagca agaaaagacc ctctcattgc ctgctaatca
       61 acccattgct ttgacgaagt tgagtttaaa tatttccccg gaagatcggg tgaaaatagt
      121 tgttactgtt tctgatggac aggcacttca tttatcacaa caatggacgc cctcttcaga
      181 aaagtcttaa tttgttgaaa tatcgagcat aagatgaatc tggagagaat ggtctgctgc
      241 gaatcagcca acctgaaagt atggataaca caaccttcaa ggatgactaa tcattgagga
      301 aatagaataa atgttcagac cttttttaga ctctcttatg ctcggcagtt tgttttttcc
      361 ttttattgcc attgcaggaa gcaccgcgca agggggcgtg atccattttt atggccaaat
      421 tgtggaaccg gcatgtgacg tcagcaccca gtcatcaccc gtagaaatga actgcccaca
      481 gaatggttct attccgggca aaacctactc cagcaaagcg ttaatgagcg gcaatgtcaa
      541 aaacgcgcaa atagcctcag taaaagtgca gtatcttgat aaacagaaaa agctggctgt
      601 aatgaacatc gaatataact aagtttctga acaactcacg ttgctgagca gaaaaatgcg
      661 atttaaccaa aaagcctgct gtacacttaa gaaacaagaa ggtgtaagga ggcgttatga
      721 aaacgcgtat tcatgttgtg cagggtgata ttaccaaact ggccgttgat gtgattgtga
      781 atgcggctaa tccgtcatta atgggaggcg gcggcgtcga tggggccatt catcgcgcag
      841 cgggtccggc cctgctggat gcctgtttaa aagtcaggca acagcagggc gattgcccta
      901 cggggcatgc cgttattacg cttgcaggcg atcttcccgc taaagccgta gtacacaccg
      961 tcgggccagt ctggcgtggt ggggaacaaa acgaagacca gcttttgcag gatgcctatc
     1021 tgaatagcct acgactggtg gcggcaaaca gctatacgtc agtggctttt cctgcaatca
     1081 gtactggggt ttatagttac cctcgtgcgg cagcggctga aatcgcagtt aaaaccgttt
     1141 cagaatttat tacccgtcac gctttacccg aacaggtata ctttgtctgt tatgatgaag
     1201 aaaacgccca cctctacgaa agactcctta cccaacaagg agatgaatga tttgccccgg
     1261 ctggcgagcg cggtgctgcc actgtgttcg caacatcccg gtcagtgtgg tcttttccct
     1321 ctggagaaaa gtctggatgc gtttgccgcc cggtatcgtc tggccgaaat ggcagagcat
     1381 acgctggatg ttcagtatta catctggcag gacgatatgt cggggcggtt actgttttcc
     1441 gccctgttag ccgcagcaaa gcgtggcgtt cgcgtccgtt tgttgctgga cgacaacaat
     1501 acgcccggac ttgacgacat tttgcgcttg cttgacagtc atccacgcat tgaagtccgg
     1561 ctttttaatc ctttctcgtt tcgcttgcta cgtccgcttg gatatatcac cgacttttcc
     1621 cgtctcaatc gccggatgca caataaaagt ttcactgtcg atggcgtggt taccctagtg
     1681 ggaggacgaa atattggtga tgcctatttt ggtgcagggg aagagccact tttttcggat
     1741 ttagatgtca tggcaatagg ccccgtggta gaggatgttg ccgatgattt cgcccgctac
     1801 tggtattgca aatcggtttc acccttacag caggtgctgg atgtcccgga aggtgaaatg
     1861 gcggatcgca tcgagttacc cgcctcctgg cataacgatg ccatgacgca tcgttattta
     1921 cgcaaaatgg aatccagtcc atttataaat catctggttg atggaacgtt gccgcttatc
     1981 tgggcgaaga cacgtttatt aagtgatgat ccggcgaaag gggagggcaa ggcaaaacgg
     2041 cattcactgt taccgcagcg cctgttcgat atcatgggct cacccagtga acgcatcgat
     2101 attatctctt cctattttgt accgacacgc gcaggtgtgg cgcaactctt acggatggtg
     2161 agaaaagggg taaagattgc gatcctaacc aattctcttg ccgctaacga tgttgctgtc
     2221 gtccatgccg gatacgcgcg ctggcgcaaa aaattgctcc gctatggcgt ggaattatat
     2281 gaactcaagc cgacgcgtga acaaagcagt acgttacacg atcgcggcat aaccggtaat
     2341 tccggtgcca gcctgcatgc taaaactttt agcatcgatg gtaaaacggt gtttatcggc
     2401 tctttcaatt tcgatccgcg ttcaacattg ctcaatactg aaatgggctt cgtgatagag
     2461 agcgaaacgc tggcacagtt aattgataaa cgctttattc agagccagta cgatgcggcc
     2521 tggcagctcc gtctggacag gtggggacgg atcaactggg ttgatcgtca tgcaaagaaa
     2581 gaggttattc tcaaaaaaga acccgccacc agtttctgga agcgggttat ggtcagactg
     2641 gcgtcgatat tgcccgtgga atggttattg taaatgtggc ttaacgggct ggtgctttat
     2701 cgttctcacg cttaacaacc ggtttaccag agaacaaaaa cttcagtaac gggatgcgta
     2761 aatgaatttc atacagaatt atcgcaatcc ctactacgaa tatcaggcca cagagaaaac
     2821 caagccagtt ggaggtgatg tgcggtgtaa tgtatgcgcc gaaaaacagc gttaacgggt
     2881 ggtgaaccag atagataaac agcgatgcgt taacaaaata agtcacccgc gctgactgga
     2941 agttaagcaa acggtggcca aaggagaaga ccacattcac catccacagg ccgaggacca
     3001 tggtgatcac cgactcggtt tcgtacatcc aggcatcgcc actgccatag cgctggttga
     3061 gtaaataagc gacaaacgcc aatgctgcag cgagggtaca gccacgagac ggcgtggtaa
     3121 acaaggcttt aagatgaggg aaaatgaaag ccagtgcgcc gaggataaag aacggtaaat
     3181 aaaatagcgt ttgcatgaca ataaaattga acatgccatt actcagaatg ggtggataca
     3241 caataaaaat cgttcttctt attaccgcat agccgatgcc gaggcataaa aaaatcaccg
     3301 atagttttac catcgagaat tttttattcg ttttatcaga attttctaaa ttatttctga
     3361 tgcgcttaaa tatccataca cacagcgtcg tcatgactac taacaccagt aaaaaccaca
     3421 ggtgtgatat taattcccag gccaacgtat tatatttgtc atacaatgat agcccaggcc
     3481 aactttccgc tttccctttg acatattgca gcataataaa ttgcggtaat gtcagtaggg
     3541 ggatggctgt taacatcggg atacctacac gttcgacacg tactttccac cattttttca
     3601 agggatagcg taaaaaaagc atgtaggaaa agtatccgga tataacgaaa aatacctgca
     3661 tgcggaacga gtggatgaag tcattaaaaa gggttagcca caatgacggt tcggcgctat
     3721 tcacatgcca tgtatggctc gaatagatta aagaaatatg aaaagggatc cctaacaaca
     3781 tcagccaggc gcggatggag tcgaggaaat attcacgttg cgcgggtact ggattcatat
     3841 atggttaact aatctcggat ttttcgtctt atccctgtcg ggttatgcct ttaggcttgt
     3901 tgccatagcg acaccgacct gaccgcgcca ggcgcaggct tcaaggtttt tatgcatagc
     3961 atcatcgcta ccactaacca gaatggaagc gtctgtaaga cggttgataa ataaatttgc
     4021 tggcaaaccc tacacgaagt cgatgcttct gtctttagga gaagcacgga aagtgaaaac
     4081 ggttgcaatc aggtgcttaa tccatgagcc agcgtgctga acgataccgg gattcagttg
     4141 tcggaatggc tggttatcca ttaaaataga tcggatcgat ataagcacac aaagggggaa
     4201 gtgcttacta attatgaaac ataaactaca aatgatgaaa atgcgttggt tgagtgctgc
     4261 agtaatgtta accctgtata catcttcaag ctgggctttc agtattgatg atgtcgcaaa
     4321 gcaagctcaa tccttagccg ggaaaggcta tgaggcgcct aaaagcaact tgccctccgt
     4381 tttccgcgac atgaaatacg cggactatca gcagatccag tttaatcatg acaaagcgta
     4441 ctggaacaat ctgaagaccc cattcaaact cgagttctac catcagggta tgtacttcga
     4501 taccccggtc aaaataaatg aagtgactgc caccgcagtc aaacgaatca aatacagccc
     4561 ggattatttc actttcggcg atgttcagca tgacaaagac acggtaaaag accttggttt
     4621 tgccggtttt aaagtgcttt acccgatcaa cagcaaagat aaaaacgatg aaatcgtcag
     4681 catgctcggg gccagctatt tccgcgtgat tggtgcaggt caggtttatg gcctttctgc
     4741 ccgcggccta gcaattgata ccgctttgcc atcgggtgaa gaatttccac gcttcaaaga
     4801 gttctggatc gagcgtccaa aaccgactga taaacgttta accatttatg cattgcttga
     4861 ctcgccgcgt gcgacaggtg cttacaaatt cgtggttatg ccagggcgtg acacggttgt
     4921 ggatgtgcag tcgaaaatct atctgcgcga taaagtcggc aaactggggg ttgcaccgtt
     4981 aaccagtatg ttcctgtttg ggccgaacca accgtcgcct gcaaataact atcgtccgga
     5041 gttgcacgac tctaacggtc tgtctatcca tgctggtaat ggcgaatgga tctggcgtcc
     5101 gttgaataac ccgaaacatt tagcggtcag cagcttctcc atggaaaacc cgcgaggctt
     5161 tggtctgttg caacgaggtc gtgatttctc ccgctttgaa gatctcgatg atcgttacga
     5221 tctccgtcca agcgcatggg tgactccgaa aggggagtgg ggcaaaggca gcgttgagct
     5281 ggtggaaatt ccaaccaacg atgaaaccaa cgataacatc gtcgcttact ggacgccgga
     5341 tcagctgccg gagccgggta aagagatgaa ctttaaatac accatcacct tcagccgtga
     5401 tgaagacaaa ctgcatgcgc cagataacgc atgggtgcaa caaacgcgtc gttcaacggg
     5461 ggatgtgaag cagtcgaacc tgattcgcca gcctgacggt actatcgcct ttgtggtcga
     5521 ttttaccggc gcagagatga aaaaactgcc agaggatacc ccggtcacag cgcaaaccag
     5581 cattggtgat aatggtgaga tagttgaaag cacggtgcgc tataacccgg ttaccaaagg
     5641 ctggcgtctg gtgatgcgtg tgaaagtgaa agatgccaag aaaatcactg aaatgcgtgc
     5701 tgcgctggtg aatgccgatc agacgttgag tgaaacctgg agctaccagt tacctgccaa
     5761 tgaataagac aactgagtac attgacgcaa tgcccatcgc cgcaagcgag aaagcagcat
     5821 tgccgaagac tgatatccgc gccgttcatc aggcgctgga tgccgaacac cgcacctggg
     5881 cgcgggagga tgattccccg caaggctcgg taaaggcgcg tctggaacaa gcctggccag
     5941 attcacttgc tgatggacag ttaattaaag acgacgaagg gcgcgatcag cttaaggcga
     6001 tgccagaagc aaaacgctcc tcgatgtttc ccgacccgtg gcgtaccaac ccggtaggcc
     6061 gtttctggga tcgcctgcgt ggacgcgatg tcacgccgcg ctatctggct cgtttgacca
     6121 aagaagagca ggagagcgag caaaagtggc gtaccgttgg taccatccgc cgttacattc
     6181 tgttgatcct gacgctcgcg caaactgttg tcgcgacctg gtatatgaag accattcttc
     6241 cttatcaggg ttgggcgctg attaatccta tggatatggt tggtcaggat ttgtgggttt
     6301 cctttatgca gcttctgcct tatatgctgc aaaccggtat cctgatcctt tttgcggtac
     6361 tgttctgttg ggtgtccgcc ggattctgga cggcgttgat gggcttcctg caactgctta
     6421 ttggtcgcga taaatacagt atatctgcgt caacagttgg cgatgaacca ttaaacccgg
     6481 agcatcgcac ggcgttgatc atgcctatct gtaacgaaga cgtgaaccgt gtttttgctg
     6541 gcttgcgtgc aacgtgggaa tcagtaaaag ccaccgggaa tgccaaacac tttgatgtct
     6601 acattctgag tgacagttat aacccggata tctgcgtcgc agagcaaaaa gcctggatgg
     6661 agcttatcgc tgaagtcggt ggcgaaggtc agattttcta tcgccgccgc cgtcgccgcg
     6721 tgaagcgtaa aagcggtaat atcgatgact tctgccgtcg ctggggcagc cagtacagct
     6781 acatggtggt gctggatgct gactcggtaa tgaccggtga ttgtttatgc ggcctggtgc
     6841 gcttgatgga agccaacccg aacgccggga tcattcagtc gtcgccgaaa gcgtccggta
     6901 tggatacgct gtatgcgcgc tgtcagcagt tcgcgacccg cgtgtatggg ccactgttta
     6961 cagccggttt gcacttctgg caacttggcg agtcgcacta ctggggacat aacgcgatta
     7021 tccgcgtgaa accatttatc gagcactgcg cactggctcc gctgccgggc gaaggttcct
     7081 ttgccggttc aatcctgtca catgacttcg tagaagcggc gttgatgcgc cgtgcaggtt
     7141 ggggggtctg gattgcttac gatctcccgg gttcttatga agaattgccg cctaacttgc
     7201 ttgatgagct aaaacgtgac cgccgctggt gccacggtaa cctgatgaac ttccgtctgt
     7261 tcctggttaa gggtatgcac ccggttcacc gtgcggtgtt cctgacgggc gtgatgtctt
     7321 atctctccgc tccgctgtgg tttatgttcc tcgcgctctc tactgcattg caggtagtgc
     7381 atgcgttgac cgaaccgcaa tacttcctgc aaccacggca gttgttcccg gtgtggccgc
     7441 agtggcgtcc tgagctggcg attgcacttt ttgcttcgac catggtgctg ttgttcctgc
     7501 cgaagttatt gagcattttg cttatctggt gcaaaggaac gaaagaatac ggcggcttct
     7561 ggcgcgttac attatcgttg ctgctggaag tgctgttttc cgtgctgctg gctccggtac
     7621 gcatgctgtt ccatacggtc ttcgttgtca gcgcgttcct tggctgggaa gtggtgtgga
     7681 attcaccgca gcgtgatgat gactccactt catggggcga agcgttcaaa cgccacggct
     7741 cacagctgct gttggggtta gtgtgggctg ttgggatggc gtggctggat ctgcgtttcc
     7801 tgttctggct ggcaccgatt gtcttctcgt tgatcctgtc accgtttgtt tcggtgattt
     7861 ccagccgtgc caccgttggt ctgcgcacca aacgctggaa actgttcctg atcccggaag
     7921 agtattcacc gccgcaggtg ctggttgata ccgatcggtt ccttgagatg aatcgccaac
     7981 gctcccttga cgatggcttt atgcacgcag tgtttaaccc gtcatttaac gctctggcaa
     8041 ccgcaatggc gacagcgcgt caccgcgcca gtaaggtgct ggaaatcgcc cgtgaccgcc
     8101 acgttgaaca ggcgcttaac gagacgccag agaagctgaa tcgcgatcgt cgcctggtgc
     8161 tgctaagcga tccggtgacg atggcccgtc tgcatttccg cgtctggaat tcccctgaaa
     8221 gatattcttc atgggtgagt tattacgaag ggataaagct caatccactg gcattgcgta
     8281 aaccggatgc ggcttcgcaa taaaaacgta gttgcctgat gcgctacgct tatcaggcct
     8341 acatcgttcc tgcaatttat tgattttgca agattttgta ggtcggataa ggcgttcacg
     8401 ccacatccgg caaaaacaac gagccaataa aaataccggc gttatgccgt aatgccagtc
     8461 agttaagcaa ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt
     8521 accactctc
//
LOCUS       DAZPFP010000161         8509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPFP010000161 DAZPFP010000000
VERSION     DAZPFP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.162"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            427..1359
                     /gene="glaH"
                     /locus_tag="ACHUR4_003284"
     CDS             427..1359
                     /gene="glaH"
                     /locus_tag="ACHUR4_003284"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1456745.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1379..2647
                     /gene="lhgO"
                     /locus_tag="ACHUR4_003285"
     CDS             1379..2647
                     /gene="lhgO"
                     /locus_tag="ACHUR4_003285"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1456746.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2670..4118
                     /gene="gabD"
                     /locus_tag="ACHUR4_003286"
                     /pseudo
     CDS             2670..4118
                     /gene="gabD"
                     /locus_tag="ACHUR4_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4126..5412
                     /gene="gabT"
                     /locus_tag="ACHUR4_003287"
                     /pseudo
     CDS             4126..5412
                     /gene="gabT"
                     /locus_tag="ACHUR4_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5650..7050
                     /gene="gabP"
                     /locus_tag="ACHUR4_003288"
     CDS             5650..7050
                     /gene="gabP"
                     /locus_tag="ACHUR4_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1456747.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7071..7733
                     /gene="csiR"
                     /locus_tag="ACHUR4_003289"
     CDS             7071..7733
                     /gene="csiR"
                     /locus_tag="ACHUR4_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1456748.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7734..8183)
                     /gene="kbp"
                     /locus_tag="ACHUR4_003290"
     CDS             complement(7734..8183)
                     /gene="kbp"
                     /locus_tag="ACHUR4_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1456749.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8267..8425)
                     /locus_tag="ACHUR4_003291"
     CDS             complement(8267..8425)
                     /locus_tag="ACHUR4_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1456750.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1935 a         2224 c         2352 g         1998 t
ORIGIN      
        1 tagaggcgga tctctaaata tcaatattcc taggcgtagc gctcttcttt tagggaaaac
       61 aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg
      121 gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat ttactgtttt
      181 ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat
      241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
      301 tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
      361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
      421 cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat
      481 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
      541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
      601 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
      661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
      721 gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
      781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
      841 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
      901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
      961 gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg cccgatgcgc
     1021 tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt gttcgacgtt
     1081 gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc aaaagatttt
     1141 gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa aggcattctt
     1201 tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg gctgcacggt
     1261 cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca gcgtggctat
     1321 ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa ttgagcggat
     1381 gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca tgcaactgat
     1441 tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc cggcctgtca
     1501 ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc ccggcagcct
     1561 gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg atcaaaacgg
     1621 cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg aaatggaacg
     1681 gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt ggataaacgc
     1741 cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt ttgtgccgtc
     1801 cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct tccaggccag
     1861 aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata aaaacggcgt
     1921 ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta gctgttccgg
     1981 gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta ttatctgccc
     2041 gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta accacctgat
     2101 ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc gcatgatcga
     2161 cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag gctatcgcaa
     2221 gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga ttcgccgggt
     2281 gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt gcaaaagcgg
     2341 ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc tccagccctg
     2401 gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg acgattttct
     2461 gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg cagcgacatc
     2521 agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg caagccagag
     2581 taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg ccgcatttaa
     2641 tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac ttattccgcc
     2701 agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc atcgacgtca
     2761 ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca gatgaaaccc
     2821 gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc accgccaaag
     2881 aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag gacgatttag
     2941 cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc gaaatcagct
     3001 acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat ggcgacacca
     3061 ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt ggcgtcaccg
     3121 ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc ggtccggcgc
     3181 tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc tctgcgctgg
     3241 cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac gtggtcaccg
     3301 gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc aaactgtcgt
     3361 ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa gacatcaaaa
     3421 aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat gccgatctcg
     3481 ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa acctgcgtct
     3541 gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa aaattacagc
     3601 aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc atcgggccgc
     3661 tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg ctggagaaag
     3721 gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc ttccagccaa
     3781 ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg ttcggccccc
     3841 tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc aatgacaccg
     3901 agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc cgcgtcggcg
     3961 aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat gaagtggccc
     4021 cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat ggcatcgaag
     4081 attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg aaggaacagc
     4141 aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg gcaaattcac
     4201 ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg ccgtgagtat
     4261 cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc gaaggtggtg
     4321 gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt gctggcttac
     4381 gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga tttcgccaag
     4441 aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa aatcgcccgc
     4501 gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg gcgcacgcat
     4561 tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg gctgatgccg
     4621 ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga ggatgacgct
     4681 atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat cgccgccatc
     4741 atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc ctttatgcag
     4801 cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga agtgcagagc
     4861 ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc gccggatctt
     4921 accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac cgggcgcgcg
     4981 gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg taacccgatt
     5041 gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcaaaaa
     5101 gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga aaaacacccg
     5161 gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt tgaagatggc
     5221 gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc ccgcgataag
     5281 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtaccactc
     5341 accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg ttttgatgag
     5401 gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt ccggcctacg
     5461 agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     5521 aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc aataacaatt
     5581 aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     5641 ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct gaaatcacgc
     5701 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     5761 gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc cggattactg
     5821 gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga taccggttcg
     5881 ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat cggctggttg
     5941 tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc catgatcctg
     6001 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta
     6061 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     6121 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     6181 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     6241 aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     6301 gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat tgtccgcgcc
     6361 accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtcgtg
     6421 gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg ctcggttctg
     6481 gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt actttccgta
     6541 accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc cttaagccgt
     6601 cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg
     6661 gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta cgcgcctgcg
     6721 aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt ttatttagtc
     6781 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     6841 ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     6901 ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta
     6961 gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg
     7021 caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta
     7081 cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg
     7141 atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt gggccgttgc
     7201 aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt
     7261 atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata
     7321 tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg
     7381 ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc
     7441 ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg ggctgtggtt
     7501 ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta
     7561 tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac gatcagcacc
     7621 agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag ttaatgcgcc
     7681 agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct
     7741 tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag catcggttta
     7801 ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca
     7861 ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt
     7921 ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac aaggattttc
     7981 tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga
     8041 atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc
     8101 gcctgatcgt ctttatcgtg ctgacctgta accgcatccc agagtttttc tccggcatct
     8161 ttaacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg
     8221 tagcagagga acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa tcgcgggttt
     8281 gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc agaatattaa
     8341 taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gatcggcggc agaatgatgg
     8401 tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact cattgtttaa
     8461 aaagagattt tatctcttaa gcgtagtaat tttagcggag cctgccagt
//
LOCUS       DAZPFP010000162         8502 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPFP010000162 DAZPFP010000000
VERSION     DAZPFP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.163"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            45..398
                     /locus_tag="ACHUR4_003292"
     CDS             45..398
                     /locus_tag="ACHUR4_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1456751.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHUR4_003293"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHUR4_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR1456752.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHUR4_003294"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHUR4_003294"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1456753.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHUR4_003295"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHUR4_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1456754.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHUR4_003296"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHUR4_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1456755.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHUR4_003297"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHUR4_003297"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1456756.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHUR4_003298"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHUR4_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1456757.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="ACHUR4_003299"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="ACHUR4_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1456758.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="ACHUR4_003300"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="ACHUR4_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1456759.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7610..8502
                     /locus_tag="ACHUR4_003301"
                     /pseudo
     CDS             7610..8502
                     /locus_tag="ACHUR4_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1905 a         2309 c         2288 g         2000 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc ctggaaaata tcggcattat cc
//
LOCUS       DAZPFP010000163         8376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPFP010000163 DAZPFP010000000
VERSION     DAZPFP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.164"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="ACHUR4_003302"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="ACHUR4_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /gene="yniD"
                     /locus_tag="ACHUR4_003303"
     CDS             1130..1237
                     /gene="yniD"
                     /locus_tag="ACHUR4_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1456760.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="ACHUR4_003304"
     CDS             complement(1290..2048)
                     /locus_tag="ACHUR4_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1456761.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="ACHUR4_003305"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="ACHUR4_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1456762.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="ACHUR4_003306"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="ACHUR4_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1456763.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="ACHUR4_003307"
     CDS             3761..4621
                     /locus_tag="ACHUR4_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1456764.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="ACHUR4_003308"
     CDS             complement(4662..5198)
                     /locus_tag="ACHUR4_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1456765.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="ACHUR4_003309"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="ACHUR4_003309"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1456766.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="ACHUR4_003310"
     CDS             6176..6766
                     /locus_tag="ACHUR4_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1456767.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="ACHUR4_003311"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="ACHUR4_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8293..>8376)
                     /locus_tag="ACHUR4_003312"
     CDS             complement(8293..>8376)
                     /locus_tag="ACHUR4_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456768.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         2173 a         2004 c         2009 g         2190 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcggcga tgcatactga ctcata
//
LOCUS       DAZPFP010000164         8134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPFP010000164 DAZPFP010000000
VERSION     DAZPFP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.165"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..953
                     /locus_tag="ACHUR4_003313"
     CDS             <1..953
                     /locus_tag="ACHUR4_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1456769.1"
                     /translation="KEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIV
                     IKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMAD
                     GTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADL
                     SFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANV
                     NFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTL
                     VTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1107..2756)
                     /locus_tag="ACHUR4_003314"
     CDS             complement(1107..2756)
                     /locus_tag="ACHUR4_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1456770.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2907..4256)
                     /gene="ghxP"
                     /locus_tag="ACHUR4_003315"
                     /gene_synonym="yjcD"
     CDS             complement(2907..4256)
                     /gene="ghxP"
                     /locus_tag="ACHUR4_003315"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1456771.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4962..5426)
                     /gene="soxR"
                     /locus_tag="ACHUR4_003316"
     CDS             complement(4962..5426)
                     /gene="soxR"
                     /locus_tag="ACHUR4_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1456772.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5512..5835
                     /gene="soxS"
                     /locus_tag="ACHUR4_003317"
     CDS             5512..5835
                     /gene="soxS"
                     /locus_tag="ACHUR4_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1456773.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5838..7415)
                     /gene="pdeC"
                     /locus_tag="ACHUR4_003318"
                     /pseudo
     CDS             complement(5838..7415)
                     /gene="pdeC"
                     /locus_tag="ACHUR4_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7844..8125
                     /locus_tag="ACHUR4_003319"
     CDS             7844..8125
                     /locus_tag="ACHUR4_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR1456774.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2260 a         2085 c         1859 g         1930 t
ORIGIN      
        1 gtaaagaaaa ggatgtatcc attaaggtgg tatattttat aaataatgtc gccgtccata
       61 ataatactat cgaaattcca cagatagtaa atggtggtta cgatttttca caccttagcc
      121 tgaagggtat cgtgattaaa gatgaagatt tatccaattc gaattttgca ggttgcagac
      181 tacaaaacgc tattttccag gactgtaata tgtataaaac gaatttttat tacgccataa
      241 tggaaaaaat actttttgat aattgtattc tcgatgactc aaatttcgct cagataaaaa
      301 tggccgacgg aactctaaat gcatgctccg ctatgcatgt tcaattctac aatgcagcaa
      361 tgaatagagc caatattaaa aataccttcc ttgactattc aaatttttat atggcgtaca
      421 tggctgaggt aaatctttat aaagtaatag cgccatatgt taatttattt aaagccgacc
      481 ttagtttctc taaactcgat ttaattaact ttgaacatgc tgatctgtct cgcgtcaatc
      541 tgaacaaagc aatcctccag aatataaact taattgatag caaactcttt tgtacgtggc
      601 tgacaaatac attcctcgaa atggttatat gtaccggctc taatatggct aatgttaatt
      661 ttaataatgc caatttaagc aactgccatt tcaactgttc tattttaaca aaagcctgta
      721 tgtttaatac ccgtctctat cgggttaatt tcgatgaggc tagcgtccag ggaatgggca
      781 tttccattct ccgtggggag gaaaatatcc ccattgatag tgataccctg gtaacactac
      841 agaaattctt tgaagaagat tgtacctctc atactggcat gtcacaaact gaggataata
      901 ttaatgcagt cgctatgaag attactgcag atattatgca acacgcagat tgaggttaag
      961 tcgaagaaca gccaatgaat tgactttaca agagagataa cttatttgtt ttagaatcat
     1021 gaatgttcgc ccgagctaac ctgttaaagg tgcaaaagat tgcacctttt tcagattacc
     1081 tcgggcctgg ttacgtcgct ttcggcttac tgattttcct caattagcaa cgcttcaagc
     1141 aaatcgagat cgtgcagtaa tttttgcagc gtttcgttgc tgatctcccg cgtggcgcgc
     1201 aggtggtaca gttcagcacg ttcagaacgc aatgccgcca gacggaagcg acgctcaagg
     1261 ttctcttcct gcacggaact ttcaacgtcg ttacgtccat cggcgcgacg acgcaggtta
     1321 ccaatgacgc gagaactgac ctccgtaagc aactggttat cgatattctc ttcggtatcc
     1381 gccgccagac gctcctccat tttctggatc gccacaatcg ccacttctgc cgttgccgct
     1441 cgcgcaatac gttcttcttt caattgttgc gaatggtccg cgacttcaat gtgttgtagc
     1501 agaataggca acatcaccac gccgacaaac agcgagaaga gaatgacgcc agccgccagg
     1561 aacaccagct catagcgcgc cgggaagacg ttaccatccg gcaagagcag cgggattgag
     1621 agcacaccgg ccagagtgat cgccccacgc accccagcga aagacgcgat caggatttct
     1681 cgtgtggtcc acgaaccaaa ctccatcggc ttcttcttca ggaagcggtt gctgaacttt
     1741 ttcatcgtcc acagccagcc gaaacggacc agcatcagcg ccgcatatat cagaataata
     1801 tcggtaaaca gcatccagat ttcgacgtta gggtcgattt ctgccgccat cagcgacgtc
     1861 tccagaatac ccggcagttg cagacctaac agcaggaaca ccatgccgtt aaagacaaat
     1921 tccagcatcg cccaggtgct gtttgcgcgc aggcgcattg ccagtggcgc acggcgcatc
     1981 acaccggagc gggtgatggt catcccggcg gcaaccgcag cgaggatgcc cgaaacgcca
     2041 atatgttcgg caatcagata agaagcgaat ggcagcaaga acagcagaac gatctgcgtc
     2101 gcgggttcat caccgcccca gcggctgagg aatcgcagcg agcgaccata cagccagctc
     2161 accacaaaac cggcaagaat accgccaatg gctactttca taaattcgac cgtcgcaccg
     2221 ccgacagtga agatcatcgt ccccatcgcc actgccacgg caaacttcaa cgacaccagg
     2281 ccggaagcgt cattcatcaa cgcttcgccc tgcaaaatgc ccatgatttt tttcgggatg
     2341 cgcccttcgc caacaatccc ggagagcgcc acagcatcgg tcggagaaag taccgccgcc
     2401 agcgcaaagg cggggatcag cggaatgccc ggcaccaccc agtaaataag gaagccaatg
     2461 ccgaccacgg tgaccaccac cagcgccagc gcgaggccga aaatctctcg accatgttcg
     2521 agaaattcac gggtcggcgt tttccagcca tcagcgaaca gcaacggcgg gataaacaag
     2581 actaaaaaga gttcaggatc aaactccaca tgcaaaccaa acgtcggcca cgccagtagc
     2641 gcaccgatgg cgatttgcat aagcggaagc gggatctgaa agggcatgac acgagtgacc
     2701 accccggaca gcgagaccac gagggtcatt atcagtatgg tgaagaagat ttccatgcgt
     2761 tccctgtttg cgatgttttt tatgatctga gaaagacgtt atgcctgaat tctcattttt
     2821 tcagaatatc gcaaaagctc acacaatgtg cggagaaaat aaaaacgggc agccattgcc
     2881 acccgttttc agaatccgat gaaagattag atagcccagc cacccgcata gaaggtcacc
     2941 agcgccacgg cgataacgac cgtaccgatg ttcaacttgc gccattcacc ggaaaccaga
     3001 cgaccaatca ccagagtcgc gaagccgatc atgatgcctg ttacgatgtt acaggtcagc
     3061 acgatgaata ccgccgtaac cagacccgcc atcgcatcaa caaaatcagc aaagtcgatt
     3121 ttcgccacgt tgctcagcat cagcaggcca acgtacatca gcgccggagc cgttgcatac
     3181 cccggaacga ggtaagaaag cggagagagg aacagaatca gcaggaacag cacgccaacg
     3241 gtgatagccg tcaacccggt tttaccgccc gccgccgtac ccgccgcaga ctcgatgtat
     3301 accgctgccg gagctgcacc caccaggcca gagaaaacgc tgctcatgga gtcagtggtc
     3361 agtgctttcc cgccgtcgat gatctgccca tctttatcca gcaggttcgc ctggccggcg
     3421 acagcgcgga tagttccggt ggcatcaaat actgccgtca tcaccagcgc cagaacgctt
     3481 ggcaggacta caggattcag cgcgcccata atgtccaggc tgccaatcag tgaattgccg
     3541 ttttcatcgc tcaatgaagg catggcgaaa acgccggaga aatggacgtt aggatcgaag
     3601 atcaaaccga caattgagat accgataatg gtcagcagaa tgccaccagg gactttcagt
     3661 ttttccaagc cgatgatcac cgccagacct gccagtgaca taatcaccgg gaaggtcgcg
     3721 aaatcaccca gcgcaacggg cagaccatca agcgggtttt taatcaccag accgacacca
     3781 ttagcggcaa tgagcagcag gaacagaccg ataccgatcc ccgtgccgtg cgccacaccg
     3841 tgcggcaagt tgcgcaaaat ccagctacgg atacccgtgg cagaaattac cgtaaacagc
     3901 acacccatca ggaacacggc acccagcgcg acaggtacgc taatatgttg ccccagcacc
     3961 aggctgaatg cggtaaacgc cgtcagggag atggcgcaac caatcgccaa cggcagatta
     4021 gcccacagac ccatcacgat agaaccgagt ccggcaacca gacaggttgc aacgaaaact
     4081 gccgcaggcg ggaagcctgc tttacccaac atacctggaa cgacgatgac cgagtagacc
     4141 atcgccagaa acgttgttaa cccggcaacc acttcctgac ggacagtgct tccacgttgt
     4201 gaaattttaa accaggcgtc gagtgaaccg ccggtacgcg ctgatggcgt agacataaga
     4261 aaacatcccc tgagagttta attttcgtca agatgcgtgg cggacaatcc cctgccagcg
     4321 aaacgttatc ttcctgctcc aggtttgcgg caatttggtg cgtcgcaaaa aagcaaacgt
     4381 ttagctccgc gcatacaaaa cgggtggtaa aaaaaagcaa acgattatcc ggcgatcata
     4441 gcggcttttt caaccgaagt ttgctgcttt tttctctttt attgtcttga cgatgaaaaa
     4501 ccaggcaatt gatgcgcaaa cgttatcgtc gatgtgcaag attgcagcgt tatcgagccg
     4561 tataccgttc tggattgcga taaaaaatcg tctgagatga caaaaatatt gcggctaaaa
     4621 agtatacttt tacactggcg aagaaacgtc ggtgacatac aattaaagca tcaacaccaa
     4681 ccggaacctc caccacgtgc tcgaatgagg tgtgttgacg tcgggggaaa ccctcctgtg
     4741 ttccagcggg atagagagaa agacaaagac cggaaaacaa actaaagcgc ccttgtggcg
     4801 ctttagtttt gtgtttgcgg ataattccaa aagagctcga tcagtcccct cgccccttcg
     4861 gggagagggt tagggtgagg ggaacaggca gcaccagtgc aaatatttac cctcaccccg
     4921 gccccatagt taacgatgag tcagcttgtc cttgtggcgc tttagttttg ttcatcttcc
     4981 agcaagcgtg cgccggtacc ttcttctcct aagcggtcgc ccgggttacg caacgggcaa
     5041 tcactgcgcg aaaggcagcc acaaccaata catccgtcca gttcgtcacg cagcgccact
     5101 aaggtatgaa tgcgccgatc caactcttct cgccattggg acgaaagctg tttccactct
     5161 ttcgcactta acgtatgccc ttctggcaac acgccaaacg cttcaccaat ggtcgccagc
     5221 ggaatgccaa tacgctgagc gattttgata attgcgacat atcgcaacac atcacgttta
     5281 tatcgccgct gattgccgct gttacggata ctagtaatca accctttgct ttcatagaaa
     5341 tgcagcgccg ataccgccac accgctgcgt ttcgccactt cgccaggggt tagcagcgct
     5401 ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact tgaggaatta
     5461 tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt tatgtcccat
     5521 cagaaaatta ttcaggatct tatcgcatgg attgacgtgc atattgacca gccgcttaac
     5581 attgatgtcg tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg gatgttccgc
     5641 acggtgacgc atcagacgct tggcgattac attcgccaac gccgcctgtt actggccgcc
     5701 gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct gggttatgtc
     5761 tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc cagcgattat
     5821 cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa ttgctccatc
     5881 cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg gcaatagcgc
     5941 acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc agcgatcgtt
     6001 tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg actggttttg
     6061 tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac attcaatgat
     6121 ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac ttcgtaacca
     6181 gcctggcgga aagccagaat taatcggcgt cattttttcg acatcaagaa atgcatgctc
     6241 agtcacttca aatttaattt gctgcggacg caccgcgtat tgctctgttt tctgattgat
     6301 tcgcgctatc aaccgtgacg tatgaaaatc ggaggccgac aggttaatag aaacatagcg
     6361 atcggcatgt gttgccaggt acgcgcccag atcgcggaag acattatcaa taacataatc
     6421 agttatctgt tctatcatcc cctccttttc tgccagcgga ataaactctg ccggattcat
     6481 tatttgcccc tgctcaccag gccaacgtaa caacgcttca gcgccgatac atttttctgt
     6541 tttgatatcg attattggct ggtaataaag acaaagttga tgtttttcga gggcgcgttg
     6601 caatttacgt ttgggagata aatagttttg tcgaatacgt agccagagta atagcagaac
     6661 aagactcccc aaaataccgg cgggcaacgc aaaaataaga tgattataaa aatgggttat
     6721 aagacgttga tatgaagtcg caacaatggc tgcaattggg cgttttgtcg aataaattgt
     6781 cgcatataaa tagccatttc tttgtacggt taaatccttc aaatgaatca gcggagaaaa
     6841 cgttgctgcc gaggcctctt tgcttaacga gaaaaaggtt ttcatcaccg tatcatacac
     6901 accccattgc aatgtcgggt catcagacat cacttcactc cagaagagag ggttgataac
     6961 cgccacataa tttccccgct gcatataggt cattttatag ccagagaaaa aaggcgtatc
     7021 gcggtaataa tagatagaaa cgttaggttc acgcttataa tcggccggtg caatcgtata
     7081 gccgtttaca ggcgctatca gcgatgagca taaaaaatgg ttatcacgaa ttcattaata
     7141 taaagatagc cacgaataat attcaacatt cgcttttgat gggctggagt gcaaacttgc
     7201 ccctgatagc gttcagctgc atcgctcact aaatctgcct ggcgaatgac cagctcggat
     7261 ttatccagtg ccagttgagc aaaggttcgg agctgattat tcacttctga ttttgcccaa
     7321 aggaaggcaa tccatagcga aagaatgatg ggaaagagaa ctaaaaagat aatgcccggc
     7381 aacgccagta attggtgtcg tgcacgatga ctcattgcta tcaatttcct gcctgtttat
     7441 acccggccat tgcaaggtga aaccctggct accgacgtct ttagataaat cttacggtat
     7501 aaatagcact tttgcctgat ttgaaattga taccttttta atcgtcacct ggatgaaaat
     7561 aattcattga tattcaaaat taattttcta caggaataca gaggcggcgg gaagatatgc
     7621 ccttaatgcc actgaaaaac cacataattt gtacagcaat aaaagtcacg gcctaatatg
     7681 gctaacaaac aatcaattgt gatatagttc acaaaattaa tgaaacaaac agagtgtttc
     7741 atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac cacgcctccc gtgaacaacg
     7801 ccgcgctgtc cagggcatcg gctcttttca gaggaattgt ttgatggcta ccctcaccac
     7861 tggcgtggtt cttcttcgct ggcaacttct tagtgccgta atgatgtttc tggccagcac
     7921 actcaacatc cgttttcgtc ggtctgatta tgtcgggctt gcggtgatca gcagcggtct
     7981 gggcgttgtt tccgcctgtt ggttcgcaat ggggttgctt ggcatcacaa tggcggacat
     8041 caccgctatc tggcacaaca tcgagtcggt gatgatagaa gagatgaatc agacaccgcc
     8101 acaatggcca atgattttga cctgatacgt agaa
//
LOCUS       DAZPFP010000165         8074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPFP010000165 DAZPFP010000000
VERSION     DAZPFP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.166"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUR4_003320"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUR4_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUR4_003321"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUR4_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1456775.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUR4_003322"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUR4_003322"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1456776.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUR4_003323"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUR4_003323"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1456777.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHUR4_003324"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHUR4_003324"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1456778.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHUR4_003325"
     tRNA            7783..7858
                     /locus_tag="ACHUR4_003325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1844 a         2083 c         2193 g         1954 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgag
//
LOCUS       DAZPFP010000166         7939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPFP010000166 DAZPFP010000000
VERSION     DAZPFP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.167"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            258..761
                     /gene="dps"
                     /locus_tag="ACHUR4_003326"
                     /gene_synonym="pexB"
     CDS             258..761
                     /gene="dps"
                     /locus_tag="ACHUR4_003326"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1456779.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1165..1911
                     /gene="glnH"
                     /locus_tag="ACHUR4_003327"
     CDS             1165..1911
                     /gene="glnH"
                     /locus_tag="ACHUR4_003327"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1456780.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2050..2709
                     /gene="glnP"
                     /locus_tag="ACHUR4_003328"
     CDS             2050..2709
                     /gene="glnP"
                     /locus_tag="ACHUR4_003328"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1456781.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2706..3428
                     /gene="glnQ"
                     /locus_tag="ACHUR4_003329"
     CDS             2706..3428
                     /gene="glnQ"
                     /locus_tag="ACHUR4_003329"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1456782.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3689..5913
                     /gene="ybiO"
                     /locus_tag="ACHUR4_003330"
                     /pseudo
     CDS             3689..5913
                     /gene="ybiO"
                     /locus_tag="ACHUR4_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5910..6836)
                     /gene="rlmF"
                     /locus_tag="ACHUR4_003331"
     CDS             complement(5910..6836)
                     /gene="rlmF"
                     /locus_tag="ACHUR4_003331"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1456783.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7112..7372
                     /gene="mcbA"
                     /locus_tag="ACHUR4_003332"
     CDS             7112..7372
                     /gene="mcbA"
                     /locus_tag="ACHUR4_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1456784.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7637..>7939
                     /locus_tag="ACHUR4_003333"
     CDS             7637..>7939
                     /locus_tag="ACHUR4_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1456785.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         1935 a         2032 c         1981 g         1991 t
ORIGIN      
        1 ctcgctactt ttcctctaca ccgtatttat atatcgaatt atgcaaaagc atatttattc
       61 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
      121 aatctttatt agttttgttt ttcatgcttg ttaccactat tagtgtgata ggaacagcca
      181 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
      241 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
      301 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
      361 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
      421 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
      481 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
      541 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
      601 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
      661 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
      721 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
      781 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
      841 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
      901 gtatccacat catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta
      961 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
     1021 atcttcaggg tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
     1081 atgagttgca aaattggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
     1141 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
     1201 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
     1261 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
     1321 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
     1381 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
     1441 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
     1501 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
     1561 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
     1621 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
     1681 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
     1741 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
     1801 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
     1861 ggaacttaca acgaaatcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
     1921 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
     1981 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
     2041 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
     2101 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
     2161 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
     2221 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
     2281 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
     2341 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
     2401 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
     2461 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
     2521 agcattaaag acacctcgct gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
     2581 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
     2641 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
     2701 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
     2761 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
     2821 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
     2881 ttgtcgatgg cctgaaggtc aacgatccga aagttgacga gcgcctgatt cgccaggaag
     2941 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
     3001 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
     3061 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
     3121 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
     3181 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
     3241 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
     3301 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
     3361 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
     3421 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
     3481 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
     3541 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
     3601 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
     3661 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
     3721 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
     3781 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
     3841 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
     3901 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
     3961 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
     4021 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
     4081 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
     4141 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
     4201 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
     4261 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
     4321 ctgaatgcag gcagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
     4381 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
     4441 cgcccgttca cgattcagga cgagagcgcc cgttactggg gtcgtcgcct gagctggtta
     4501 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
     4561 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
     4621 tacctgatct tccgaaataa aaaagagatt acccagcata tgctcaactt tgcggagcat
     4681 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
     4741 agcgcctatt ttatcatgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
     4801 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
     4861 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
     4921 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
     4981 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
     5041 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
     5101 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
     5161 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
     5221 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
     5281 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
     5341 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
     5401 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
     5461 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
     5521 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
     5581 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
     5641 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
     5701 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
     5761 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
     5821 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
     5881 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
     5941 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
     6001 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
     6061 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
     6121 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
     6181 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
     6241 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
     6301 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
     6361 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
     6421 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
     6481 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
     6541 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
     6601 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
     6661 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
     6721 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
     6781 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
     6841 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
     6901 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
     6961 cagcattatg catgtcattg atttgtctta ttttgtaaaa atatatcgtt caaaaaataa
     7021 gcatttctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
     7081 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
     7141 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
     7201 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
     7261 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
     7321 ttccgcaggc ggaaatgacc agatgttagg tactgcaacc atctacaaat aaccccacca
     7381 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
     7441 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
     7501 atcaagtgat aatgcttatc aaaattatta tcacttgcac gaacactatc acgggattaa
     7561 cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga
     7621 aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct
     7681 cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga
     7741 agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc
     7801 gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat
     7861 gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct
     7921 caaaaacgtc cccggcgtg
//
LOCUS       DAZPFP010000167         7925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPFP010000167 DAZPFP010000000
VERSION     DAZPFP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.168"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHUR4_003334"
     CDS             complement(<1..956)
                     /locus_tag="ACHUR4_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR1456786.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUR4_003335"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUR4_003335"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR1456787.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUR4_003336"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUR4_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUR4_003337"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUR4_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1456788.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUR4_003338"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUR4_003338"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1456789.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHUR4_003339"
     CDS             complement(5018..6346)
                     /locus_tag="ACHUR4_003339"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1456790.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..7925)
                     /locus_tag="ACHUR4_003340"
                     /pseudo
     CDS             complement(6354..7925)
                     /locus_tag="ACHUR4_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         2102 a         2008 c         1787 g         2028 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctga
//
LOCUS       DAZPFP010000168         7778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPFP010000168 DAZPFP010000000
VERSION     DAZPFP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.169"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(162..647)
                     /gene="sixA"
                     /locus_tag="ACHUR4_003341"
     CDS             complement(162..647)
                     /gene="sixA"
                     /locus_tag="ACHUR4_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1456791.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(850..2994)
                     /gene="fadJ"
                     /locus_tag="ACHUR4_003342"
     CDS             complement(850..2994)
                     /gene="fadJ"
                     /locus_tag="ACHUR4_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1456792.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2994..4304)
                     /gene="fadI"
                     /locus_tag="ACHUR4_003343"
     CDS             complement(2994..4304)
                     /gene="fadI"
                     /locus_tag="ACHUR4_003343"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1456793.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4484..4768)
                     /locus_tag="ACHUR4_003344"
     CDS             complement(4484..4768)
                     /locus_tag="ACHUR4_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1456794.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5140..6480
                     /gene="fadL"
                     /locus_tag="ACHUR4_003345"
     CDS             5140..6480
                     /gene="fadL"
                     /locus_tag="ACHUR4_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1456795.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6846..>7778
                     /gene="yfdF"
                     /locus_tag="ACHUR4_003346"
     CDS             6846..>7778
                     /gene="yfdF"
                     /locus_tag="ACHUR4_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR1456796.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPE"
BASE COUNT         2065 a         1989 c         1788 g         1936 t
ORIGIN      
        1 aatcaagata cgcgtagaag aataatttcc gaatgaatgt agaaagtgtt ttatttatag
       61 ataaggagag tcttcgatag tagtgaggtt gacagatatc cgctgcctaa tgagcaatta
      121 tgcccaatga atctacctca ttgggcataa tttggaacac atcagatagc ttttgccatc
      181 ttcagattac acgggctcat ctgccagttg aatgtaccgt ttccgctttc atcaagcgtt
      241 acgctggcaa tggccgaggt ggtaaacata ggcggcgttt cgcccggaca taactcggca
      301 accagatacc cgactaacgg taagtgagag atcaccagta ccgaagcaac accttcattg
      361 gtcaacgcct gcaaataggc actgaccaga ccaacatcgc cgcagggcgt taactccggc
      421 agaacttctg cactggaagg caggttcaga caatccccta cttcttccag tgtttgctcg
      481 gctcgcagga acgggctcac cagaacgcgt tcgatttcca ctttttgacc tttcagccag
      541 ttcgccatca ggcgagattc gtcacaacca ttagtggtca gaggacgaac cgaatcactg
      601 gcggaatcga gggctgcgtc gccgtgacgc atgataaaaa cttgcatatt gcaccgcttt
      661 tgttaaccag attcgcccgt tttctttcat ctgaaaccag agaaagaaaa cggtggccgg
      721 cattgtgcct tatccattca ccgaatgaaa cgctgttttt tacctcaatg gcgtaagtat
      781 agtcaatatg cgtttacatt ttgaccaaca ctccgccatt cagcgcggat tcatatagct
      841 ttgaccttct tattgcaggt cagttgcagt tgttttccaa aaactttccc cacgcgcgcc
      901 catctcgacc aaacgctcgc aaggggtaaa acgggaacca tactgcgtgg ccagtcgttg
      961 cattattgca accacttcgc ccgcgccgag agaatcgata tagcggaacg gtccaccgag
     1021 aaatggcgga aaaccaatgc caaataccgc accaatatcc ccgtcacgca cgctacggat
     1081 aacctgctca tcaacacaac gtactgcttc attcagcatc aacatcacac accgttcagc
     1141 aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca atgagcgggt aaatggcagg
     1201 atcgacctgt tttttgcttt tacgcccttt ctgaccataa agatagaaac cccgaccatt
     1261 ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca acatttgcag gcgcgctaaa
     1321 acgttctcca taagcggctt ccagtacagg aataatttta gtcccggtgt cgattcctac
     1381 ctcatccaaa agttggattg ggcctaccgg aaaaccaaat ttcactagcg cggcatcaat
     1441 gtgctctacc cgttcacctt cggtcagcat gcggatagct tcattaatgt aaggcgctaa
     1501 gatgcgattg acgtaaaacc cggctttgtc acgcacgaca atgggcgttt tgccctgttt
     1561 tttcgccagt tttaccgtag tggcgatggt ttgcgccgat gtacccgcat gaggaataat
     1621 ctctaccagc ggcatttttt ccaccggact gaagaaatgc aggccaataa cttgctcagg
     1681 tcgcgcggcg tgagcggcga tatcaccaat cggtaaagat gacgtgttcg aagcaaaaat
     1741 ggtatgagcg gcgcaatttt gctccacttc cgccaccatc tgttgtttta attcgagatt
     1801 ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag ccgcgatagt ccgtcgttcc
     1861 ggagattaat gccagctgtt tgtcacgttc gctggctttg agatgacgac ggcgaacttt
     1921 gccctccagc tgatcccaac tgtacttcag cgcatgattt atgccctgcg ggttgatatc
     1981 tttaattctg accggaagcc ccgctttaca agcagtgaca taagcaatac cgccgcccat
     2041 caagccacca cctaaaattc ccacgctgtt taatggcgca ggcggtgcat cactaccggg
     2101 atctttcttc acgtccgtac tggcaaaaaa gatactacgc aaagcttgcg attgcggcgt
     2161 catcgccagt tcgccaaagg cgcgagcttc agcgtcatag ccgctgctgg tgccctgcgc
     2221 taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc ggataattgc cttgagtttt
     2281 gtgttctgtt ttcttgccga ccattttgaa cagcagcgca cgacctaacg gccccgccag
     2341 aatacgctcg cgtacaggta gagggcggga agatgggcga tccttcttcg ccagctcaac
     2401 agcggcttcc agcaaaatgg agtgcggaac aacgtcatcc accagcccca gctttaatgc
     2461 ctgtttcgcc cgaagttgtt ttccggtgag aatcatctct aatgctgtac tgacgcctat
     2521 cagacgcggt aaacgctggg tgccgcctga accgggtaac aatccaagtt gtacttcagg
     2581 caaaccgagc accgttttag gatcgtcagt acaaacgcga ccgtggcacg ccagcgccag
     2641 ctccagccca ccgcccaggc aagcgccatg aatagccgcg ataaccggaa tgggcaaagc
     2701 atgaatctcc gccatcaact gttggccctg ccgcgccagt gcttccgctt cttgcgccgt
     2761 tttgcagttg ccaatcatgt tgatgtctgc tccggcgata aaattgtccg gtttcgcgga
     2821 gataaacacc acgccgcgca actctttgtt ttcacggagt tgcttaataa tggcgcgcac
     2881 ctgcgaggca aattccgcct tcagggtatt cattttctca tccggtacgt cgatggtgat
     2941 gatagcaatg ttgtccagac gaacattaag agtaaacgct gatgccattt ccattattcc
     3001 gcctccagaa ccattgccgc gccaagccca ccggcagcac aggcggtaac taaaccaaat
     3061 ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa tcatccgcgc gccggtcgcc
     3121 gcgaagggat gtccgtaagc aatcgaaccg ccaagcacgt taaatttgct atcgtccact
     3181 tcgccagtgg catgtgcacg ccccagtact tcacgagcaa aacgttcact acccagcaac
     3241 tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca tatcgatcaa tgtcagttcg
     3301 ctcatcgtca aaccggcacg ttccagcgcc agcggtgttg accaggctgg accgagcaac
     3361 atgtcctgcc agacatcaat cgcagtaaat gcgtagctgc gcagataccc cagcggcacc
     3421 agccctaatt ctttcgcccg ggattcagtc atcaggatca ccgctgccgc gccatcggtc
     3481 agcggcgtac tgtttgccgc cgttaccgtt ccgtgtttgc gatcaaacgc cgggcgcagc
     3541 tttgcgtaat cggcaagcga ggaattaccg cgaatattgt tgtcttcgac aagcggttgt
     3601 ttataaggag ggataaaggc agtcatcacc tcttctttga gttttccgtc tgaccatgcc
     3661 tgagcggcac gctgatgcga acggtgcgct aatgcatctt gctgttctcg ggtgatgccg
     3721 taggttttcg ccatttgttc ggcggtgtcg cccatccgca agccagtaga atattctgct
     3781 accgcaggcg gtacgggcat taagtcgcgc aaacgcaggc gagagaagag tttcaggcgc
     3841 tggctcatgg ttcgagcttt gttgacatca accagcacgt acgccagttt tttactgacg
     3901 ccaattggca atacggaaga ggaatctgcc ccaccggcaa tccccgctcg gatagttccc
     3961 gccatcaggc tttctgcgac gtttgcaacc gcttggaaac tggtagcgca agcgcggctg
     4021 acgctgtaag catcggtatg cacattcatt ccggtaccga gaacaatttc acgcgcaatg
     4081 ttgggggctt caggcatttg tacgacctga ccaaagacca gctgttcaat cacttcagcg
     4141 gggatctcgc tgcgtgccag cagttcgcct accaccatct tccctaaatc aaccgtggga
     4201 atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac gtaaaccgct aacaatggcg
     4261 atacgatcgc cctggcgggt aaccagtggt aaaacctgac ccataacact cccctgtaaa
     4321 aaaataaata aagtggtctg acctgatcat agtcttaacc atttttttac atttagccaa
     4381 gcggagaaaa aggaaagtgg gagctatgac acagtgaaga cagaagaaag aaaagaaaac
     4441 gcccctgcca gcaactgaca ggggcgtacg tacccgagag gaattaacgc agacccagct
     4501 ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg cgcacaccgt
     4561 cagactcttc agtgaaagtc ggggagattt tgcacggttc ggatttcacg ctacgggatt
     4621 tttcagtcag cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac acgcggctgt
     4681 aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag cagcaaaccg
     4741 gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc acccaaggtg
     4801 ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt tgttaattct
     4861 gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa aattcacccg
     4921 aatccgcgag tgcgcaacct ccaaattttg ccagctggat cgcgtttctt agatcatatt
     4981 tgaaaaaaga tggaaacata cttgcaacat tctagctggt ccgacctata ctctcgccac
     5041 tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt acagcacgta
     5101 acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa aaccctgttt
     5161 acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg gtcggcaggc
     5221 tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg ggaaggcgca
     5281 attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat gtttgaccgc
     5341 ccgacatttt ctgcgggtgc ggtttatatt gacccggatg tgaatatcag cggaacgtct
     5401 ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt tccgaacatg
     5461 cactttgttg caccgattaa cgaccaattt ggttggggcg cttctattac ctctaactat
     5521 ggtctggcaa cagagtttaa tgatacttat gcaggcggct ctgtcggggg tacaactgac
     5581 cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct taaataatgc atggagcttt
     5641 ggtcttggtt tcaacgccat ctacgctcgc gcgaaaattg aacgtttcgc aggcgatctg
     5701 gggcagttgg ttgctggtca gattatgcaa tctcctgccg ggcaaactcc tcaagggcaa
     5761 gcattggcag ctaccgccaa cggtatcgac agtaatacca aaatcgctca tctgaacggt
     5821 aaccagtggg gctttggctg gaacgccgga atcctgtatg aactggataa aaataaccgc
     5881 tatgcactga cctaccgttc tgaagtgaaa attgacttca aaggtaacta cagcagcgat
     5941 cttaatcgtg cgtttaataa ctacggtttg ccaattccta ccgcgacagg tggcgcaacg
     6001 caatcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcagg ttataaccgt
     6061 gttgatccac agtgggcgat tcactatagc ctggcttaca ccagctggag tcagttccag
     6121 cagctgaaag cgacctcaac cagtggcgac acgctgttcc agaaacatga aggctttaaa
     6181 gatgcttacc gcatcgcgtt gggtaccact tattactacg atgataactg gaccttccgt
     6241 accggtatcg cctttgatga cagcccagtt ccggctcaga atcgttctat ctccattccg
     6301 gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa agatgcttca
     6361 gtcgacgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga aggcccatac
     6421 cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta cgcgttctga
     6481 taacgcgttc gcctggataa agtcacctgc tatgcaggtg actttaactc ccccacttca
     6541 ccgaaagtag tttcctcatt gcttgcctcc tgagttttgc aaacagcctg atggcagctt
     6601 gccctttcaa atcagtaagt ggtatccaca gacaaacctg aagggaaatg catattttca
     6661 agcgttctgc tcgtccttct caaagagttt acttttctgc atttccagga tactcaaccc
     6721 ccctggctat tgtgcgctta tacactcaaa ttaaagatag gttctaaata aatgagcgtt
     6781 ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca acatggaata
     6841 aaatcatgct accatctatt tcaatcaaca ataccagcgc agcttaccca gaatccatca
     6901 atgaaaataa caatgatgaa attaatggat tagtacaaga gttcaaaaac ctttttaatg
     6961 gtaagaaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
     7021 gagtaaacga cgatccttat agatctaata ttaataattc ctcagttact tatattgata
     7081 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
     7141 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
     7201 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
     7261 atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
     7321 aatttgatga gagcgggaaa agcgaaccaa ctgacttatt tacctggtac ggtaaagata
     7381 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatctc
     7441 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
     7501 gtctcaccca atattgtagt gaaaacgcca ggagtgcttc agcgtggttt gaaagcagta
     7561 aagctatcat ggcagaatca tttgcaactg gttccgatca tcaggttgta aacgagctca
     7621 acggggaaag actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat
     7681 ataattttca agtggacgat gcaaaattta gacgcgataa tgtaaaagaa ataatttcta
     7741 ctttattcgc taacaaagtt gacgttgacc atcctgaa
//
LOCUS       DAZPFP010000169         7745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPFP010000169 DAZPFP010000000
VERSION     DAZPFP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.170"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            71..835
                     /locus_tag="ACHUR4_003347"
     CDS             71..835
                     /locus_tag="ACHUR4_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1456797.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            855..1793
                     /locus_tag="ACHUR4_003348"
     CDS             855..1793
                     /locus_tag="ACHUR4_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1456798.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1849..3138
                     /locus_tag="ACHUR4_003349"
     CDS             1849..3138
                     /locus_tag="ACHUR4_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1456799.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            3135..3428
                     /locus_tag="ACHUR4_003350"
     CDS             3135..3428
                     /locus_tag="ACHUR4_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1456800.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            3485..5131
                     /gene="fadK"
                     /locus_tag="ACHUR4_003351"
     CDS             3485..5131
                     /gene="fadK"
                     /locus_tag="ACHUR4_003351"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR1456801.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(5188..7566)
                     /gene="ppsA"
                     /locus_tag="ACHUR4_003352"
     CDS             complement(5188..7566)
                     /gene="ppsA"
                     /locus_tag="ACHUR4_003352"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1456802.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT         1861 a         2024 c         2032 g         1828 t
ORIGIN      
        1 cgcacactat tttcaggcca tttttacctt ccatcggaga tggttccgta tgcgactcac
       61 aggagaaatc atgaaaataa taacctgctt taagctggtg cctgaagaac aggacattgt
      121 tgtcactcca gaatacaccc tgaatttcga caatgccgac gccaaaatca gccagttcga
      181 tctcaatgcc attgaagctg caagccagct cgcaacagat gacgatgaga tagccgcgct
      241 gaccgttggc ggctcattgt tgcagaactc gaaagtgcgc aaagacgtgc tatcccgcgg
      301 gccgcacagc ctgtatttgg tgcaggatgc gcaacttgaa catgcactgc ctctcgatac
      361 cgcaaaagcg ctggcggcag caattgaaaa gatcggcttc gatttactga tctttggtga
      421 aggttccggc gacctttatg cccagcaggt tggcttgctg gtcggagaaa ttctgcaact
      481 tccggtgatt aatgcagtga gtgctattca gcgtcagggc aatacactgg tgattgaacg
      541 cacgcttgaa gatgatgttg aagttattga actctctgtt ccagccgtgc tctgcgtcac
      601 ctccgatatt aacgtgccac gcattccttc gatgaaagcc attctcggcg cgggtaaaaa
      661 accggtaaat cagtggcagg caagtgatat tgactggagc cagagcgcgc cacttgctga
      721 actggtcggc attcgcgtac cgccgcaaac agaacgtaag cacatcattc tcgataacga
      781 ttcgccggag gccattgccg agctggcgga acatctgaag aaagccctga actgaagcct
      841 acggagaaga aacgatgagt caattaaaca gcgtctgggt ctttagcgat aatcctgaac
      901 gttatgctga actgtttggc ggcgctcagc aatggggcca acaggtgtat gccattgtac
      961 aaaataccga ccaggcgcag gcagttatgc cttatggtcc aaaatgtatt tatgttcttg
     1021 agcaaaacga cgcgctgcaa cgcactgaaa attacgccga aagcattgct gccctgctga
     1081 aagataaaca ccccgctatg ttgctgttgg ccgcgacgaa acgtggtaaa gctctggcag
     1141 cacggttaag tgtgcaactg aatgcggcgc tggtgaacga tgccacggcg gtggatattg
     1201 tcgatggtca catttgcgcc gaacaccgga tgtatggcgg gttagcgttc gctcaggaaa
     1261 agatcaacag cccgctggcg atcattaccc ttgcacccgg tgttcaggaa ccgtgcacca
     1321 gtgatacctc tcatcagtgc ccgacagaaa cggtacctta tgttgctccg cgtcatgaaa
     1381 ttctctgtcg cgaacgccgt gcgaaagccg caagcagcgt ggacctgagc aaagcaaaac
     1441 gtgtggttgg cgtcggtcgt ggtctggcgg cgcaggatga cctgaaaatg gtccacgaac
     1501 tggcggcggt gctgaatgct gaagtcggct gttcacgtcc aattgccgaa ggcgagaact
     1561 ggatggagcg tgaacgttat atcggtgtct ccggcgtgtt gctgaaatcc gatctctacc
     1621 tgacgctggg gatctccggg cagatccaac atatggttgg cggcaacggc gtaaaagtga
     1681 ttgtcgccat caataaagat aaaaatgcgc caatcttcaa ctatgccgac tacggtctgg
     1741 tgggcgatat ctacaaagtc gtccctgccc tgattagcca gttgagccgc taattcccct
     1801 ttcaaccaca tctcccgctg taaacgcagc aggaagggag cataacgcat gtcggatgac
     1861 aaatttgatg ccattgtggt cggtgcgggc gttgctggta gcgttgccgc actggtcatg
     1921 gcacgagccg ggctggatgt cctggtgata gaacgcggcg acagtgccgg atgtaaaaac
     1981 atgaccggcg ggcgtcttta tgcccacaca cttgaagcaa tcattccagg ctttgcagta
     2041 tcagcgccgg tagaacgcaa ggtcacacgc gagaaaatct ccttcttaac cgaagaaagc
     2101 gccgttaccc tcgattttca ccgcgagcaa ccagatgttc ctcaacacgc atcttatacc
     2161 gtattgcgta atcgtctgga cccgtggttg atggaacaag ccgagcaggc gggcgcgcag
     2221 tttatcccag gcgttcgcgt cgacgcgctg gttcgtgaag gaaacaaggt cactggcgtg
     2281 caggctgggg atgatattct cgaagcgaat gtggtgattc tggctgatgg cgttaactcg
     2341 atgcttggcc gctcgctggg aatggttccc gcttccgatc cgcatcatta cgctgttggt
     2401 gttaaagagg ttattggcct cacaccagaa cagatcaacg atcgctttaa tattacgggc
     2461 gaggaaggtg ccgcctggct gtttgccggt tccccttctg acggcctgat gggcggggga
     2521 tttctctata ccaacaagga ttccatatcc ttggggctgg tttgtggatt gggtgatatc
     2581 gcccatgcgc aaaaaagcgt gccgcaaatg ctggaagatt ttaaacaaca ccccgccatt
     2641 cgcccgctga ttagcggcgg caaactgctt gaatattccg cgcatatggt gccggaaggc
     2701 ggtctggcaa tggtgccgca actggttaac gagggcgtga tgatcgttgg tgacgccgca
     2761 ggcttctgcc tgaatttggg ttttacggtc cgcggcatgg atttagccat tgcatcggct
     2821 caggctgccg ccacaacggt gatcgccgcc aaagaacgcg cggatttctc cgccaacagt
     2881 ctggcgcaat acaaacgtga gctggaacaa agctgcgtca tgcgtgatat gcagcatttt
     2941 cgcaagatcc cggcgctgat ggaaaacccg cgcctgttta gccaataccc acgaatggta
     3001 gccgacatca tgaacgagat gttcaccatt gacggcaaac caaaccagcc ggtacgcaaa
     3061 atgatcatgg gacacgcgaa gaaaattggg ctgatcaact tgctgaaaga tggcattaag
     3121 ggagcaaccg cgctatgagc cagaacgcta cggttaacgt cgacatcaaa ttaggcgtca
     3181 ataaattcca tgttgatgag ggccacccgc atatcattct gacggaaaat cccgatatca
     3241 atgaattccg taaattaatg aaagcctgcc ctgccggact ttataagcag gatgacgtag
     3301 gaaacattca ttttgatccc gccggttgtc tggagtgcgg cacctgtcgg gtgctgtgcg
     3361 gtaacactat tctcgaacag tggcaatatc ccgcaggcac cttcggtatt gattttcgct
     3421 acggctaatc atgcatccca caggcccgca actcgggcct gatgttctgt ttcgagagtc
     3481 caaaatgaaa gtgacattaa cgtttaacga acaacgtcgt gcggcgtatc gtcagcaagg
     3541 gctatggggc gatgcttcac tggccgatta ctggcagcag accgctcgtg cgatgccaga
     3601 caaaattgcc gtggtcgata atcatggtgc atcgtacacc tatagcgcgc tcgatcacgc
     3661 cgcgagctgt ctggcaaact ggatgttagc gaagggtatt gaatcaggcg atcgcatcgc
     3721 atttcaactg cctggctggt gtgaatttac cgttatctat cttgcctgcc tgaaaatcgg
     3781 tgcggtttcc gtgccgctgt tgccttcctg gcgggaagca gaactggtgt gggtgctcaa
     3841 taagtgtcag gcaaaaatgt tctttgcacc gacgttgttt aaacaaacgc gtccggtaga
     3901 tttaatcctg ccgctgcaaa atcagcttcc acaactacaa caaattgtcg gcgtggacaa
     3961 actggctccc gccacctctt ccctctcatt aagtcagatt atcgccgaca atatcccact
     4021 gaccacggcg ataacgaccc acggcgatga attagctgcg gtgctgttta cctccggaac
     4081 cgagggtctg ccaaagggcg tgatgctaac gcataacaat attctcgcca gtgagcgggc
     4141 ttattgcgcg cggctgaatc tgacctggct ggatgtcttt atgatgcctg cgccacttgg
     4201 tcacgcaacg ggctttctgc atggcgtaac ggcaccattc ttaattggcg ctcgcagcgt
     4261 gttgttagat attttcactc ctgatgcgtg tctcgcgctg cttgagcagc agcgttgcac
     4321 ctgtatgcta ggcgcaacgc cgtttgtcta tgatcttttg aatttactag agaaacaacc
     4381 cgcagacctt tcagcgctgc gcttctttct ttgcggcggt accacaatcc ccaaaaaagt
     4441 ggcgcgcgaa tgccagcagc gcagcattaa attattaagt gtttatggtt ccacagaaag
     4501 ttcgccgcat gcggtggtga atctcgataa ttctttgtcg cgctttatgc ataccgatgg
     4561 ttacgctgcc gcaggtgtag agattaaagt ggtcgatgac gcacgcaaga ccttaccgcc
     4621 aggttgcgaa ggtgaagaag catcgcgtgg ccccaatgtg tttatggggt attttgatga
     4681 acctgaatta accgcccgtg cgctggatga agaaggctgg tattacagcg gcgatctctg
     4741 ccgtatggat gaggctggct atataaaaat taccggacgc aaaaaagata ttattgtccg
     4801 cggcggcgaa aatattagca gccgtgaagt ggaagatatt ttattgcagc atcctaaaat
     4861 tcacgatgcc tgtgtggttg caatgcccga tgaacgttta ggtgaacgat catgcgctta
     4921 tgtcgtgctg aaagcgccgc atcattcatt atcgctggaa gagatggcgg ctttttttag
     4981 ccgtaaacgg gtcgcaaaat ataaatatcc tgaacatatc gtggtaatcg aaaaactacc
     5041 gcgcactgcc tcagataaaa tacaaaagtt tttgttaaga aaagatatta tacggcgatt
     5101 aacgcaggat gtctgtgaag agattgaata agtttcatct tcggggatca cataaccccg
     5161 gcgactgaac gccgccgggg atttatttta tttcttcagt tcagccaggc ttaaccaggt
     5221 ttgcaccacg gtgtccgggt tcagagacag gctatcgatc ccctcttcca tcaaccatgc
     5281 ggcaaagtct tcgtggtcgg acggaccctg accgcaaatc ccgacatatt tgccctgttt
     5341 cttcgcggca cggatagcca tcgacagcag tgctttcacc gcatcgttgc gctcatcgaa
     5401 caattcagac accacgccgg agtcacggtc cagaccgagc gccagctgcg tcatatcgtt
     5461 tgagccaatt gagaagccgt cgaaatattc gaggaactgc tcggccagca aggcgttgga
     5521 cgggatttca cacatcatga tgattttcag cccgttctcg ccacgtttca gcccctgacg
     5581 cgccagttct tcaaccaccg ctttcgcctg atctacggta cgcacgaacg ggatcatgat
     5641 ctcaacgttg gtcagcccca tgtcgttgcg cacacgtttc actgcttcac actccagtgc
     5701 gaagcagtcg cggaagctgt cggaaacata gcggcccgca ccacggaagc cgagcatcgg
     5761 gttctcttca tctggctcgt aacgctcacc accgaccagg ttggcatatt cgttcgattt
     5821 aaaatcagag agacggacaa tgacgcgctt cggataaaac gcggcaccca gcgtcgcgat
     5881 cccttcagtc agacgaccaa cgtaaaattc acgcggagaa tcaaaacctt tcatcatctc
     5941 gcggatttcg ttttgcaact gcggttcctg atcgtcaaac tcaagcagtg cgcgtgggtg
     6001 gacgccaatc atacggttga tgataaattc cagacgcgca aggcccacgc cttcgctcgg
     6061 caggcaggcg aagtcgaaag cacggtccgg gttaccgacg ttcatcatca ctttcaacgg
     6121 cagatccggc atcgtttcta cgctggagct tttcacgcta aattccagca actccgcata
     6181 gacgtaaccg gtatcacctt cggcacaaga aacagtgacg ttctcaccgt ctttcatccg
     6241 ttctgttgca tctccacagc ccactaccgc cggaatgccc agttcacgag cgatgatcgc
     6301 cgcgtgacag gtacgaccgc cacggttggt gacgatggca gatgctttct tcatgatcgg
     6361 ttcccagtcc gggtcggtca tgtcagtaac cagcacgtcg ccaggttcga tgcggttcat
     6421 ttcgctgatg tcatggatga ctttcaccgg acccgcgccg atgcgatgac cgatagcacg
     6481 gccttcggcg ataatcttac cctgtgaatg cagcgtataa cgctccatga cctgaccgcg
     6541 tgagcgcacg gtttccggac gcgcctgcac aatgaacagt ttgccggtgt ggccatcttt
     6601 cgcccactca atatccattg ggcgaccgta gtgtttctca atttgtacgg cctgttttgc
     6661 cagttcctgc acttcttcgt tggtcagcga gaagatgtca cgctgttcct gcggtacgtc
     6721 ttcgatttta acctgcttgc cgtgctcctg ggtcggcgcg taaaccatgc ggattttttt
     6781 cgaccccatg gtgcggcgca cgatagccgg gcgattcgcc gccagtgtcg gtttatgcac
     6841 gtaaaactca tccgggttaa ccgcaccctg cacgaccatt tccccaaggc cccatgcgga
     6901 agtgataaac accacctggt caaagccgga ttcggtatca atggagaaca tcacgccaga
     6961 agatgcgagg tcggagcgca ccatccgctg aacaccggcg gagagcgcca cgccacggtg
     7021 atcgtaaccc tggtgcacac gataagagat ggcgcgatcg ttaaacagag aagcaaatac
     7081 atgtttcact gccacgagaa cggcgtcaaa accctgaacg ttgaggaagg tttcctgctg
     7141 accggcaaag gaagcgtccg gcatgtcttc tgcggtggcg gaggagcgta ccgcaaaaga
     7201 ggcgttttcg tcatcggcgg aaagctgtgc ataggcttcg cgtatggcgt tttccagctc
     7261 aggctggaag ggagtgtcga taatccactg gcggatttgc gcgcccgctt tcgcaagctg
     7321 agtaacatcg tcaatatccg ttttatccag cagttcataa atgcgctggt ttacgccgct
     7381 ttggtccaga aactggttaa acgcgtcggc ggttgtggcg aaaccattcg gaacggaaac
     7441 acccattccg gaaagattag taatcatttc acccagggag gcatttttgc ccccaaccct
     7501 gtctacatca ttcatgccga gttggttata ccaaagcacc agcggtgacg agccattgtt
     7561 ggacatcgaa caatcctttt gtgataaatg aacggtttga gaaacacatt tctgcgcatt
     7621 tatctttgca tatttaaccg gatgaaaaaa acggtgaatc gttcaagcaa atatattttt
     7681 ttacttttta agactgatcc cagcgttgcg caaacctgct ctattcgaca atttccacat
     7741 aaacc
//
LOCUS       DAZPFP010000170         7646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPFP010000170 DAZPFP010000000
VERSION     DAZPFP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.171"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUR4_003353"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUR4_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR1456803.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUR4_003354"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUR4_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1456804.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUR4_003355"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUR4_003355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1456805.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUR4_003356"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUR4_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUR4_003357"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUR4_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1456806.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUR4_003358"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUR4_003358"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1456807.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUR4_003359"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUR4_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1456808.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHUR4_003360"
     CDS             7490..7639
                     /locus_tag="ACHUR4_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456809.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
BASE COUNT         1783 a         2005 c         1924 g         1934 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatt
//
LOCUS       DAZPFP010000171         7591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPFP010000171 DAZPFP010000000
VERSION     DAZPFP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.172"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(114..1585)
                     /gene="yeeO"
                     /locus_tag="ACHUR4_003361"
                     /pseudo
     CDS             complement(114..1585)
                     /gene="yeeO"
                     /locus_tag="ACHUR4_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1746..1821
                     /locus_tag="ACHUR4_003362"
     tRNA            1746..1821
                     /locus_tag="ACHUR4_003362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1779..1781,aa:Asn,seq:gtt)
     gene            complement(1859..2809)
                     /gene="cbl"
                     /locus_tag="ACHUR4_003363"
     CDS             complement(1859..2809)
                     /gene="cbl"
                     /locus_tag="ACHUR4_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1456810.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2911..3828)
                     /gene="nac"
                     /locus_tag="ACHUR4_003364"
     CDS             complement(2911..3828)
                     /gene="nac"
                     /locus_tag="ACHUR4_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1456811.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4156..4231
                     /locus_tag="ACHUR4_003365"
     tRNA            4156..4231
                     /locus_tag="ACHUR4_003365"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4189..4191,aa:Asn,seq:gtt)
     gene            complement(4287..5222)
                     /gene="ldtA"
                     /locus_tag="ACHUR4_003366"
     CDS             complement(4287..5222)
                     /gene="ldtA"
                     /locus_tag="ACHUR4_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1456812.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5284..6363)
                     /gene="cobT"
                     /locus_tag="ACHUR4_003367"
     CDS             complement(5284..6363)
                     /gene="cobT"
                     /locus_tag="ACHUR4_003367"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1456813.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6375..7118)
                     /gene="cobS"
                     /locus_tag="ACHUR4_003368"
     CDS             complement(6375..7118)
                     /gene="cobS"
                     /locus_tag="ACHUR4_003368"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1456814.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7115..>7591)
                     /gene="cobU"
                     /locus_tag="ACHUR4_003369"
     CDS             complement(7115..>7591)
                     /gene="cobU"
                     /locus_tag="ACHUR4_003369"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1456815.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2097 a         2052 c         1647 g         1795 t
ORIGIN      
        1 gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa
       61 agcctgtttt ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc
      121 gacacaactg gctttttttc acacttttgc ggctcggatc gagggtattt ccacagccaa
      181 cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt
      241 cccatccaga caccaaccac accccagcca agcatgattc cctgcacata accgactaca
      301 acccggcaac cccacatgct caacatagaa acccacatgg cgtaacgggc atcacgagca
      361 cccttaaatc cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt
      421 agctaaatca gaatcacaac gacgtgttta acctgtggat cctgggtgta aaacgatgcc
      481 ataaccccgg caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc
      541 cagaacacat gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg
      601 cctgtaatga tcgtagaagc agaaccgagc gcacttccgg gtaagttgat aagagccgca
      661 attgagaacg cgataaaatt tccggcaata acgctggtcc ccatcccggc aacgaacatt
      721 tgggttaata accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc
      781 cccatgactt cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc
      841 cttagcgcag gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa
      901 cgagaaatgg ttaaacccaa cccagccccg accaggagaa aaggccgtaa atcaatatgc
      961 cgctaataat aatattaaga atattcaggc taccgttaat cagtagcggt attttcgtat
     1021 tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagcggct ggataactga
     1081 gtacggtcag ctccagataa gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg
     1141 cgacgaaatc aataatttgt tcgccaaaat gatgaattag cgttgccaac agtacggcaa
     1201 acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct
     1261 taccgagact aaatgctaca acgacagtag taccaagatc gatagctgca aaaaaagcca
     1321 taatgaccat attgaagctg tccgccaacc ccacgccggc catcgcatct tttcccagcc
     1381 aactgaccag aaaagtgctc aggaccccca tcaacaggac acaggcgttc tccatgaaga
     1441 taggaacagc aagcggggtt atctcgcgct agaacaacac tttgtagctc ttgcgtttag
     1501 agtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc aaagccgacc
     1561 ttaattgcag aaagtgaaac cacatttcaa atgatgaggg agaatcagca aagctgcaaa
     1621 gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt
     1681 tgggaaattt aaaaatcggg tttgacaaaa tatttttcgc cgttaagatg tgcctcaaca
     1741 gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     1801 cgagtccagt cagaggagcc aaatttctga aaagcccgct tttatagcgg gctttttgct
     1861 atatctgata atcaatttcc tcttcactgt tttccatcac ctggcgcttg atatcctcaa
     1921 ctgacagtcc tgcattacaa agttccagaa agcgccaaac atagttacgc tgaagttgtc
     1981 ctcgcttcag tcccaaccag acagtattag catcgaaaag atgccgcgta tccaggcgga
     2041 ttaaattctc ttcctcttgt tcgccgctgg attgttcggc aactaatccg atcccaagcc
     2101 caagagcaac ataggtttta atgacatcag aatcctgtgc acttaataca atatctgccg
     2161 gcaaaccttt gcgggtaaat gcgtcatcag tgcgtgagcg ccccgtaatt ccctgtcggt
     2221 aagtaattaa cggccacttc gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg
     2281 gatgatcgag tggaacaagc aaactatggt gccaacaaaa ccatgggaag gcgacgagct
     2341 gcgggtcatt actcaaacgc tcgctggcga taccaatatc agcttcgcca ttttgcaaca
     2401 acgtcgcaat ttcctgtggc gaaccctgga ttagctcgag ccgaacctcc gggaaaagtt
     2461 cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa
     2521 tagtgagaac accagacgta tcgttggtaa acaggtctgc aagccgacga acattactgg
     2581 cttcattcag aatacgttct gcaatgacca gtaatgcttt gcccggttca gtcatcccca
     2641 gcagtcgctt acctcgtcga acaaatattt cgatgccaaa ttcatcctcc agttcccgaa
     2701 tatgacggct aacgcctgac tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt
     2761 aatcctgacg tgcagcctcg cggattatct ttagttgttg gaaattcacg gtaaactccg
     2821 ggcagttcag atatcccgtt attgttaaag tctaatgccc ggcataacaa ataataaaaa
     2881 cccgcatctt attccatccc gatataacgc ttagctcacc aattgccact gccttttttc
     2941 catcactgga gaactaatca ctgacattaa caactctttc actgcctgtg cctgtggtga
     3001 caagtttgct ctggcgggta aatttaagga caaagagaga ctcattgaag gagtggtaat
     3061 gcgtgacatc cacccattta ctgcgccaca taacgaacgc gcagccgatt caggtaatac
     3121 tgcaacgccc atgccgctgg caatcgctgc ggtaagcgtg gcaatagact caatttcacc
     3181 aatgactttt gccgtgagcc gccgtaggga aaaagcctca tcaacacgaa gtctaacagc
     3241 gctgtaatca ctggggagaa aaaggttcat ttgcgcaata gcattaacat caacgttttg
     3301 ccccgggcaa tcttgagttc ctaccaggaa aagatcttct ttcagcaaag cctgactgga
     3361 taccccagcc acaggggaat gctcataaat caccgccata tcgagttggt gatttatcag
     3421 tttttcgtta agcactgcac cactattttc atgaagatag ataacgacct ccgggaattc
     3481 agcgcgaacc gcctgtaata agggcatggt gatggatgac gcagcggttc ctggcgcaaa
     3541 gccaatcgag acttgccccg ataatgactg accaacgtta tgcaccgcca gttgggcctg
     3601 ctcacactga cgtaaaatgg cccgtgcatg ggtatagaga atttttccgg cgtctgttgg
     3661 cgtaacgccc cgttttgtgc gaatcaaaag ctgttgattt aactcacctt ccagtatggc
     3721 aacctgttgg ctgagcgctg gttgtgcgat atgcaatact tcagcagcct gggtcaggct
     3781 accaatatct acaattttta cgaagtattt caggcgtctg aagttcatgt tgcctccggt
     3841 ttttaagaat cggcccaagt gccgccatta cttacaacca gtatgcaaga tgcctgccag
     3901 ttctattttg gcgttgatgc gcaagctaac caactgtcaa ataagagatt atgatagatt
     3961 cgtcattggc cctttttatc agcagtcgca ttcacctgcc ctataaaagg agggtatgca
     4021 ccacgatggt tcattaccca ataagattga aagctcacca ctttgttgaa attggcagca
     4081 aacaaacaaa aaaaatgcat ttcacccttt gacatcacca tgcactgcca ttaatatgcg
     4141 ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     4201 gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aagcctgctt tcgagcaggc
     4261 tttttacttt ctaattacca acgctcttaa aacatctgtc ttgaaccaga actaatttgc
     4321 acaggcattc ccgatcgatg ttgcaacgca gcatttgcgc gatttacatc aacttcttgc
     4381 ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg gtcagactca
     4441 tattctgcac gattgcgcga cagcggctca tgaacttcca gccactttga accatccggt
     4501 tccgttgtgt atttcactgg ctgatcaata atttgtacac gagtgcctac aggaacatta
     4561 tcaaacagat atttgatatc gtcattacgc agacgaatac agccctgact tacacggagc
     4621 ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat ataaatcgcg
     4681 tacagcccca tggggttatc gggccccgca ggaacaaatg cgggcaaact ctcccctcgt
     4741 ttcgcatatt cgcgccggat gttcggcgtt ggcgtccagg ttggtgcttc ttgtttacgt
     4801 tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat accaatagga
     4861 aagacttcca ctgtattact gtctggcgga tagtaataaa gacgcatttc agcgacgtta
     4921 accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat ggtgagttgt
     4981 gagccaggct tcggcaaaaa aacatcagcg cccggattcg cttccagcat attacttaac
     5041 ccttgcccgt attgtgcggc aaaagtctcc agcggctgag tattgtgatc aggaacagtt
     5101 acagtaagcg actgccccac taaacggcta ccctctggag gtaatggata agttaccgcc
     5161 aggctatttt ggctggcaaa aagcagagca aatgagcaaa gaatatttac acgacgcatc
     5221 atgtcccttt cctaagtcgc gaatactatc cgataagtat agcttttatc agacttttcg
     5281 tttttagctg ttcaaatcag aagtcgtatt ccccggtaga acaatattac tggcagcaag
     5341 ttcgcccatg ttgttgtata tcgcacaagc agcttcgatg atggacatcg ccagagctgc
     5401 accactcccc tcacctaaac gcatatccat attgagataa ggctccagcc ccaaatgcga
     5461 gagcgctata cgcgcgcctt tttctgccga caagtgagaa ggaatgagat acggtttgat
     5521 tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaagaaatc catccagcaa
     5581 cacgggtaaa ccgcaggaag cagcacctaa catcactcca gctattccga ccaaatcaaa
     5641 tccacccact tttgccagga catcaacacc atcctgagga tttggttgat tcaacgtaat
     5701 cgcccgacgc acaacatcaa ttttattagc cagtttatct gtcggcaggt ttgcgccaat
     5761 cccaaccact tcttcaggat cccggccagt gattgtgctg actattgccg ctgccggtgt
     5821 cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt tcgccagctc
     5881 ccgcgtataa catatgacgt ccaaaagcaa cttttcagcc tgacggcgac tcattgccgg
     5941 agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagcc cggggatagg
     6001 ctcagcagta tcaataccaa catcaattac gtggacgtta gcgcccgctt gtgctgccag
     6061 cacacacacg ccggttgttc cacgggtcat attttcagcc tgtatggctg tcacttcttt
     6121 tggagaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa ccagtaccgc
     6181 ttttttgccc acatgcggta tgccattcac ccccggcatt cctgccagtt gtatggcaag
     6241 cgcctccagc tttcctaggc taccaacagg tttgaataac ccgtcaacat gccgttgtgc
     6301 acgcgacata gcggcagaat tgatggctgg gatcgtattc agtaaatcgg caattatttg
     6361 catgtcacgt ttctttatag cagtgccagc aggaagacca gttcaccaag ctctatggct
     6421 gctcccagcg tatcgcccgt ttgcccgccc agcgtacgtt tgagaagctg accgaggatg
     6481 aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccag caataatacg
     6541 gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc aattttgcca
     6601 atgaacacat tgccaagccc ttcttcccgc gcataacgat gacgatacat caataatgcg
     6661 gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg ttcgccacgc
     6721 agagccaact cgcttaacac cagaatcttt gccagtacca caaaaatcaa tgccagaccg
     6781 ccgtgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg actacgtgca
     6841 gaaaatacgc catcacaggt atcagcgagg ccgtccagat ggaattcccc ggtcatcagc
     6901 gccagcacaa gtacgctaaa cagtgccgcc agtggtgcgc cacaccatgc ctgcagcacc
     6961 atgaagacca gcccgctaat cgcgccaagc aataatccaa tcaaaggaaa agtaataata
     7021 ccgcgagaat aatgttcgaa atccagtccc tgggaccagc gacgcggtac aggcagacgc
     7081 gtaataaatg agagcatcgc ccaaaataat ttgctcattt gatttttact ccaatacccg
     7141 aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc cgcccggcaa
     7201 tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc acttcgttag
     7261 tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac tgaatctcag
     7321 cattaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta tcgccgccat
     7381 aatcaaacaa cagattagtc accattgtgg taacgcattc cagcaacaca gcctcattcg
     7441 ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact gtgcgccagt
     7501 gctccgggcg gctttgccga tgatgttcta tccgtgcagc catctcatca tcaaggattt
     7561 gcgaggtagc gatatacaga acctgtgaag a
//
LOCUS       DAZPFP010000172         7537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPFP010000172 DAZPFP010000000
VERSION     DAZPFP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.173"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUR4_003370"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUR4_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUR4_003371"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUR4_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1456816.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUR4_003372"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUR4_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUR4_003373"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUR4_003373"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1456817.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUR4_003374"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUR4_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1456818.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUR4_003375"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUR4_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1456819.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHUR4_003376"
     CDS             complement(6512..7204)
                     /locus_tag="ACHUR4_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1456820.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1826 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAZPFP010000173         7526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPFP010000173 DAZPFP010000000
VERSION     DAZPFP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.174"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUR4_003377"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUR4_003377"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1456821.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUR4_003378"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUR4_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1456822.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUR4_003379"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUR4_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUR4_003380"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUR4_003380"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1456823.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUR4_003381"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUR4_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1456824.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUR4_003382"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUR4_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1456825.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2096 a         1762 c         1716 g         1952 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttatt
//
LOCUS       DAZPFP010000174         7518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPFP010000174 DAZPFP010000000
VERSION     DAZPFP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.175"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            296..1333
                     /gene="dusA"
                     /locus_tag="ACHUR4_003383"
     CDS             296..1333
                     /gene="dusA"
                     /locus_tag="ACHUR4_003383"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1456826.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1467..1709
                     /gene="pspG"
                     /locus_tag="ACHUR4_003384"
     CDS             1467..1709
                     /gene="pspG"
                     /locus_tag="ACHUR4_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1456827.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(1875..2858)
                     /locus_tag="ACHUR4_003385"
     CDS             complement(1875..2858)
                     /locus_tag="ACHUR4_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1456828.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2941..4356
                     /gene="dnaB"
                     /locus_tag="ACHUR4_003386"
     CDS             2941..4356
                     /gene="dnaB"
                     /locus_tag="ACHUR4_003386"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1456829.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4409..5488
                     /gene="alr"
                     /locus_tag="ACHUR4_003387"
     CDS             4409..5488
                     /gene="alr"
                     /locus_tag="ACHUR4_003387"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1456830.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5741..6934
                     /gene="tyrB"
                     /locus_tag="ACHUR4_003388"
     CDS             5741..6934
                     /gene="tyrB"
                     /locus_tag="ACHUR4_003388"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1456831.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7437..>7518)
                     /locus_tag="ACHUR4_003389"
     CDS             complement(7437..>7518)
                     /locus_tag="ACHUR4_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456832.1"
                     /translation="LQKIFVDLEKKYQVLLQVKFFTHLLI"
BASE COUNT         1807 a         1815 c         1944 g         1952 t
ORIGIN      
        1 ttctctatca atgctattag cagggagata tatcaccaga gtttaatgtg tgatttttta
       61 tttatcgtcg aacctggatt gtttatcatt ggccttaaca aagttaacgg ctaataagat
      121 tatttccatc acttcgtgag agcttcatgc cttgagagga tctcaatttt cttttgcaat
      181 gagacaggcg cttcctgttg ttatggtata gtaccccgct attgagcctc ctgaacagtg
      241 aagctgaata acataacccc atgatatatc gataaaataa tctctacatt tgaaaatgca
      301 cggtaattct gaaatgcaaa aaatcaacca aaccagcgca atgcctgaaa aaactgacgt
      361 tcactggagt ggtcggttta gtgttgcacc aatgctcgac tggacggaca gacattgccg
      421 ctatttcttg cgtctgcttt cccgcaatac gttgctttat accgaaatgg tgaccacagg
      481 ggcgataatt cacggtaaag gtgattatct ggcgtacagt gaagaagaac atccggtagc
      541 gttgcaactg ggcggtagcg atccggcggc gctggcgcag tgtgcaaagc tggcagaagc
      601 gcgcggatat gatgagatca acctgaatgt cggctgcccg tctgaccggg tgcagaacgg
      661 catgtttggt gcgtgtctga tgggtaatgc gcagctggtt gccgactgcg tgaaagcgat
      721 gcgcgatgtg gtgtcgattc cggtgacggt gaaaacgcgt attggcatcg acgaccagga
      781 cagctatgaa tttctctgcg atttcatcaa taccgtttcc ggcaaaggcg agtgtgagat
      841 gttcatcatc cacgcacgta aagcctggct ttcggggtta agcccgaaag aaaaccgtga
      901 aatcccgccg ctcgattatc cgcgtgtgta tcaactgaag cgtgacttcc cgcatctgac
      961 aatgtcgatt aacggtggta tcaagtcgct ggaagaagct aaagcgcatt tgcaacatat
     1021 ggatggcgtg atggtcgggc gcgaggcgta tcagaatccg ggtattctgg cggcggtaga
     1081 ccgggagatc tttggttcct cggataccga tgccgatccg gtggcagtag tgcgtgccat
     1141 gtatccgtac attgagcatg aactcagcca ggggacgtat ctcggccata ttatccggca
     1201 tatgctgggt ttgttccagg gtattcctgg cgcgcggcag tggcggcgtt atttaagtga
     1261 aaatgcccat aaagcgggtg ctgacattaa cgtgctggaa cacgcgctca aactggtggc
     1321 ggataagcgt taacttttca ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg
     1381 cggggcgttt tgctgttaaa tcaatagatt atttttggca tgattcttgt aatgccagca
     1441 agagatttca tatttgggag agcatcatgc tggaactact ttttgtgatt ggcttttttg
     1501 tcatgctgat ggtcaccggc gtttcgttgc tgggcattat cgccgcgctg gttgtggcga
     1561 cggccattat gttcctcggc ggtatgctgg cattgatgat taagttgctg ccgtggttac
     1621 tactggcgat tgcggtggtg tgggttatta aggcgattaa agcaccaaaa atgccgaaat
     1681 atcagcgtta tgaccgctgg cgttactaag ggattgtgcg gatgatcaca acctaaggtt
     1741 ttatccttag aacaaaatag gaattgataa tcaaatctgt cactattgcg cctctaacag
     1801 attcatcgtg ctgtacccta catacagccg aactataaaa agaaagggct tcccaggtgg
     1861 aagccctatt tcttttatgg aatcagcagg ctggaacctt gcgtcgcccg gctttccaga
     1921 atctcatgcg cacgctgcgc atccttcagc ggatatttct gctgctcggc gacatcgacc
     1981 ttaatcacac cgctggcaat caaagagaac agttcattac tggcctcggt taattcctcc
     2041 cgcgtggtga tatagccttg cagggaaggg cgtgtcacat acaacgagcc tttttgattg
     2101 agaatgccta agttcacacc ggtaaccgca cctgatgagt tgccaaaact gaccattaag
     2161 ccgcggcgtt gcaggcaatc cagcgaccgt tcccaggtgt ctctgcccac ggaatcgtac
     2221 actacgcgca ctttcttacc gccggtgatc tctttcaatc gctcgaccag attctcttca
     2281 cgatagttaa taacctgcca cgcgccggct tttagcgcgc tctgcgcttt ttgcgcggtt
     2341 cctacggtgc cgatgagttt cgcgcccagg gcttttgccc actggcaggc aattaagcca
     2401 atgccgccag ccgctgcgtg gaacaggaac tgttcatcgg gtttaatttc ataggttttg
     2461 cgcagcagat aataaaccgt taagcctttc aggaaggatg ccgcagcttg ctcaaaagaa
     2521 attgccgcag gcagaatcgc cgctttatcc gcattaatgt tatgcacaga gctgtaagcg
     2581 cctaacgccg actgcgcata gactacatga tcgccagcct taatatgctt tacaccactg
     2641 ccgactttac tcacgatgcc tgccgcttcg gtacctaatc cgctgggtag cgatggcggc
     2701 gggtaaaggc cgctgcggat atacgtgtcg ataaaattga tgccgatggc tttattttcg
     2761 acctggattt cattctccgc cggatcggca ggagtgaact ctacggcttg aagtacttcc
     2821 gggccaccgt gcttgtgaaa ttcaattcgt gttgccatgt gtcctccaga acgtatcgtc
     2881 agggtctgct tcatatggta aagtttcgac ccattcttta tttcggtaac tccattcact
     2941 atggcaggaa ataaaccctt caacaaacag caggctgaac cccgcgaacg cgatccacaa
     3001 gttgccgggc tgaaagtgcc tccgcactcg atcgaagcgg agcagtcggt gttgggcggt
     3061 ttaatgctgg ataacgaacg ctgggatgat gtagccgagc gtgtggtagc agacgatttt
     3121 tacacccgcc cacaccgtca tatctttact gaaatggcgc gtttgcagga aagcggtagt
     3181 cctatcgatc tgattaccct tgcggaatcg ttggaacgcc aggggcaact cgatagcgtc
     3241 ggtggttttg cttatctggc agagctgtca aaaaatacgc caagtgcggc gaacatcagt
     3301 gcttatgcgg acatcgtgcg tgaacgtgcc gttgtccgtg agatgatctc ggttgcgaat
     3361 gagattgctg aagccggttt tgatccgcag gggcgtacca gcgaagatct gctggacctt
     3421 gctgaatccc gcgtctttaa aattgccgaa agtcgtgcaa acaaagacga agggccgaag
     3481 aacatcgccg atgtgctcga cgcaaccgtg gcgcgtattg agcagttgtt tcagcagcca
     3541 cacgatggcg ttaccggggt aaacaccggt tatgacgatc tcaacaaaaa aaccgctggc
     3601 ttgcagccgt cggatttgat catcgtcgcc gcgcgtccgt cgatgggtaa aacaacattt
     3661 gcgatgaacc tcgtcgaaaa cgcggcaatg ttgcaggata aaccagtact tatcttctcg
     3721 ctggagatgc catcagaaca gatcatgatg cgttctctgg cgtcgctgtc gcgcgttgac
     3781 cagactaaaa tccgtaccgg gcagctcgat gatgaagact gggcgcgcat ttccggcacc
     3841 atgggtattt tgctcgaaaa acgcaatatc tatatcgatg actcttccgg cctgacgcca
     3901 acggaagtgc gttcccgcgc acgccgtatt gcccgtgaac acggcggcat cgggcttatc
     3961 atgatcgact acctgcaact gatgcgcgta ccggcgcttt ccgataaccg tacgctggaa
     4021 attgcagaaa tctcccgctc gctgaaagca ctggcgaaag aactgaacgt gccggtggtg
     4081 gcgctgtccc agttgaaccg ttctctggaa caacgtgccg acaaacgtcc ggtcaactcc
     4141 gacctgcgtg aatctggctc tatcgagcag gatgcggact tgatcatgtt tatctatcgt
     4201 gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg cggaaattat tatcggtaaa
     4261 caacgtaacg gcccaatcgg gacggtacgc ctgaccttta acggtcaatg gtcgcgcttc
     4321 gacaactatg cggggccgca gtacgacgac gaataataat tattttatga attaggtaat
     4381 taaagcaaac aattatcaag gaacacaaat gcaagcggca actgttgtga ttaaccgccg
     4441 cgctctgcga cacaacctgc aacgtcttcg tgaactggca cctgccagta aaatggttgc
     4501 ggtggtgaaa gcgaacgctt atggtcacgg tcttcttgag accgcgcgaa cgctccccga
     4561 tgctgacgcc tttggcgtag cccgtctcga agaagctctg cgactgcgtg cggggggaat
     4621 caccaaacct gtactgttac tcgaaggctt ttttgatgcc agagatctgc cgacgatttc
     4681 tgcgcaacat tttcataccg ccgtgcataa cgaagaacag ctggctgcgc tggaagaggc
     4741 tagcttggac gagccggtta ccgtctggat gaaactcgat accggtatgc accgtctggg
     4801 cgtaaggccg gaacaggctg aggcgtttta tcatcgcctg acccagtgca aaaacgttcg
     4861 tcagccggtg aatatcgtca gccattttgc gcgcgcggat gaaccaaaat gtggcgcaac
     4921 cgagaaacaa ctcgctatct ttaatacctt ttgcgaaggc aaacctggtc aacgttccat
     4981 tgccgcgtcg ggtggcattc tgctgtggcc acagtcgcat tttgactggg tgcgcccggg
     5041 catcattctt tatggcgtct cgccgctgga agatcgctcc accggtgccg attttggctg
     5101 tcagccagtg atgtcactaa cctccagcct gattgccgtg cgtgagcata aagccggaga
     5161 gcctgttggt tatggtggaa cctgggtaag cgaacgtgat acccgtcttg gcgtagtcgc
     5221 gatgggctat ggcgatggtt atccgcgcgc cgcgccgtcc ggtacgccag tgctggtgaa
     5281 cggtcgcgaa gtaccgattg tcgggcgcgt ggcgatggat atgatctgcg tagacttagg
     5341 tccacaggcg caggacaaag ccggggatcc ggtcatttta tggggcgaag gtttgcccgt
     5401 agaacgtatc gctgaaatga cgaaagtaag cgcttacgaa cttattacgc gcctgacttc
     5461 aagggtcgcg atgaaatacg tggattaatc gttctgtaat atttgattgt ctgtgccgga
     5521 tgcggcgtga atgccttatc cggccaataa aatcctaaaa attcaataag ttgatgttct
     5581 ttcatgctct tataaaggtc gtgcctctgg cggatgtacg tttatcatta gtctcactct
     5641 gttgctaatt gccgttcgct cctgaacatc cactcgatct tcgccttctt ccggtttatt
     5701 gtgttttaac cacctgcccg taaacctgga gaaccatcgc gtgtttcaaa aagttgacgc
     5761 ctacgctggc gacccgattc ttacgcttat ggagcgtttt aaagaagacc ctcgcagcga
     5821 caaagtgaat ttaagtatcg gtctgtacta caacgaagac ggaattattc cacaattgaa
     5881 agccgtggcg gaggcggaag cgcgcctgaa tgcgcagccc catggcgctt cgctttattt
     5941 accgatggaa gggcttaaca gctatcgcca tgccattgcg ccgctgctgt ttggtgcgga
     6001 ccatccggta ctgcaacaac agcgcgtagc aaccattcaa acccttggcg gctcaggggc
     6061 attgaaagtg ggcgcggatt tcctgaaacg ctacttcccg gaatcaggcg tctgggtcag
     6121 cgatcctact tgggaaaacc acgtagcaat attcgccggg gctggattcg aagtgagtac
     6181 ttacccctgg tatgacgaag tgactaacag cgtgcgcttt aatgacctgt tggcgacgct
     6241 gaaaacatta cctgcccgca gtattgtgtt gctgcatcca tgttgccaca acccaacggg
     6301 tgccgatctc actaatgatc agtgggatgc ggtgattgaa attctcaaag cccgcgagct
     6361 tatcccattc ctcgatattg cctatcaagg atttagtgcc ggtatggaag aggatgccta
     6421 cgctattcgc gccattgcca gtgctggatt acccgctctg gtgagcaatt cgttctcgaa
     6481 aattttctcc ctttacggcg agcgcgtcgg cggactttct gttctgtgtg aagatgccga
     6541 agccgctggc cgcgtactgg ggcaattgaa agcaacagtt cgccgcaact actccagccc
     6601 gccgaatttt ggtgcgcagg tggtggctgc agtgctgaat gacgaggcat tgaaagccag
     6661 ctggctggcg gaagtagaag agatgcgtac tcgcattctg gcaatgcgtc aggaattggt
     6721 gaaggtatta agcacagaga tgccagaacg caatttcgat tatctgctta atcagcgcgg
     6781 catgttcagt tataccggtt taagtgccgc tcaggttgac cgactacgtg aagaatttgg
     6841 tgtctatctc atcgccagcg gtcgcatgtg tgtcgccggg ttaaatacgg caaatgtaca
     6901 acgtgtggca aaggcgtttg ctgcggtgat gtaatgcagg aaagcaggct ggagttaccc
     6961 agcctgcagt gaaattaaac tgtcgtcgct ttcactattt ctttgtagat gatttttttg
     7021 atgccgttgt tctacgtgag agataataaa acgttgtcag ttcttttatt gttaagttta
     7081 tcccaattat ctggaattcc tttccctatt ttttcgtgtg gagtaataat ttctgcacat
     7141 ctaaccatta ttaaatttaa taataagtat cgggtttgtt acgatagaat atttctatat
     7201 cctcaagaat atgctttgcc ttattgcctg tgactttctg agattcaatt atagtatcgt
     7261 taaattctaa tgttaaagag aactcttttt tccgctttgt gtaagtttaa ccttatattg
     7321 attaccaata tcaaataaaa aggtcctgta atgagaataa tcacctttaa cctcctcggc
     7381 aaaacagcaa agcgtgcaaa tatacataaa gtctttctta cccctgattt tgttaactaa
     7441 atcaataaat gcgtgaaaaa ctttacttgc aatagaactt gatacttctt ctccaggtct
     7501 acaaaaatct tttgcaat
//
LOCUS       DAZPFP010000175         7464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPFP010000175 DAZPFP010000000
VERSION     DAZPFP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.176"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(228..1628)
                     /gene="asnS"
                     /locus_tag="ACHUR4_003390"
     CDS             complement(228..1628)
                     /gene="asnS"
                     /locus_tag="ACHUR4_003390"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1456833.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1797..2999)
                     /gene="pncB"
                     /locus_tag="ACHUR4_003391"
     CDS             complement(1797..2999)
                     /gene="pncB"
                     /locus_tag="ACHUR4_003391"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1456834.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3265..5880
                     /gene="pepN"
                     /locus_tag="ACHUR4_003392"
     CDS             3265..5880
                     /gene="pepN"
                     /locus_tag="ACHUR4_003392"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1456835.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6087..6854)
                     /gene="ssuB"
                     /locus_tag="ACHUR4_003393"
     CDS             complement(6087..6854)
                     /gene="ssuB"
                     /locus_tag="ACHUR4_003393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1456836.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6851..>7464)
                     /gene="ssuC"
                     /locus_tag="ACHUR4_003394"
     CDS             complement(6851..>7464)
                     /gene="ssuC"
                     /locus_tag="ACHUR4_003394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1456837.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1893 a         1986 c         1898 g         1687 t
ORIGIN      
        1 tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa actaatccgc
       61 aataagagta cgggttattt tttgtgcgta aatgttgcaa tgtaatattc cgtaacagga
      121 tgatcgttat ggcgggggtg caaatagatt gcttgactta acaaacagat aacttgacag
      181 aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca gaagctggcg
      241 ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc agttacgtaa
      301 gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc gtagcgacgc
      361 agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat acgctcgtcc
      421 agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat gcccggagcc
      481 agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata gaacgcttta
      541 atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc cgccagataa
      601 cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa cttcctgccg
      661 cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc ggcttcaatg
      721 aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa tttcatgtcg
      781 tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc agccagaccc
      841 gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa ttccgccagg
      901 tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata aattttggat
      961 aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa agactcttta
     1021 ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag gttttccaga
     1081 tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc ggtaatcagt
     1141 ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc ctgcgccagc
     1201 gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag gtgagcgact
     1261 tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc tggatcttca
     1321 acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc ctggcccggc
     1381 gatgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg caggacgtct
     1441 tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa gcaggaaccg
     1501 tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg tacccatccg
     1561 cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc tacaggcaca
     1621 acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc cacccgtaaa
     1681 tgggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt cgcaaatgca
     1741 gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat tagctccctg atgatattaa
     1801 ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac aaacgcttta
     1861 tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt accgttacac
     1921 tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca ggtcaggcga
     1981 gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag ctcaaccgct
     2041 ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc aattcccagc
     2101 ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga gtcatgacgc
     2161 aggccctgat accgactagc gaactcgaca ccgaaatcac gcaggaaagc atccatagtg
     2221 atgcagtcgg ttaatgcaat gccaagttgg tcgggatact cttccagcca ggcagcaagt
     2281 gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg gaaccattcg
     2341 tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta gttgctggtg
     2401 cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac ttcgcgagaa
     2461 aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag accggcggtt
     2521 aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc aacgtcggcc
     2581 tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg aacttcccag
     2641 aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt atcgttggac
     2701 acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata gtcggcctta
     2761 aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag gtgctgcatc
     2821 gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc acctcggcaa
     2881 cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg ctgcatatgc
     2941 aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa ttgtgtcata
     3001 ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt caggaactgc
     3061 tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc cacctttctg
     3121 gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt ttgtacctga
     3181 aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa caccagactc
     3241 tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata ccgtcacgat
     3301 tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct cgacgcgcaa
     3361 aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga tgctccgctt
     3421 cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga gccgtggacc
     3481 gcctggaaag aagaagaaga gggcgcactg gtcatcagta atttgccgga gcgttttacg
     3541 cttaagatca ttaatgaaat aagcctggcg gcgaatacgg cgctggaagg gctttatcag
     3601 tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt tccgccatat tacgtattat
     3661 ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa ttattgccga taaaatcaaa
     3721 tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag gcgaactcga aaacggacgc
     3781 cattgggtac agtggcagga cccgttcccg aaaccgtgct acctgtttgc gctggtggca
     3841 ggcgactttg atgtactgcg cgataccttt accacgcgtt ctggtcgcga agtagcactg
     3901 gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt gggcgatgac ctcgctgaaa
     3961 aactccatga aatgggatga agaacgcttt ggcctggagt atgacctcga catctatatg
     4021 atcgtcgcgg tggatttctt caatatgggc gcaatggaga ataagggtct gaatatcttt
     4081 aactccaaat atgtgctggc ccgcaccgac accgccaccg acaaagatta cctcgatatt
     4141 gaacgcgtta tcggccatga atatttccat aactggaccg gtaaccgagt gacctgccgc
     4201 gactggttcc agctcagcct gaaagaaggt ttaaccgtct tccgcgatca ggaattcagc
     4261 tctgaccttg gttcccgcgc agttaaccgc atcaataatg tgcgcaccat gcgcggattg
     4321 cagtttgcgg aagacgccag cccgatggcg cacccaatcc gcccggacat ggtcattgag
     4381 atgaacaact tctacaccct gaccgtttac gagaagggcg cggaagtgat ccgtatgatc
     4441 cacaccctgc tgggcgaaga aaacttccag aaagggatgc aactctattt cgagcgtcat
     4501 gatggtagcg cagcgacctg tgacgacttt gtgcaggcga tggaagatgc gtcgaatgtt
     4561 gatctctccc atttccgccg ttggtacagc cagtccggta cgccggttgt gaccgtcaaa
     4621 gacgactaca atccggaaac tgagcagtac accctgacca tcagccagcg cacgccagcc
     4681 acgccggatc aggcagaaaa acagccgctg catattccgt ttgccatcga attgtatgac
     4741 aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc cggtgaattc cgtgctgaac
     4801 gtcacccagg cggaacagac ctttgttttt gataatgtct acttccagcc ggtgcctgcg
     4861 ctgctgtgcg aattctctgc gccagtgaaa ctggaatata agtggagcga tcagcaactg
     4921 accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt gggatgcggc gcaaagtttg
     4981 ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc aagggcagcc gctgtctctg
     5041 ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg atgagaagat tgatccggcg
     5101 ctggcggcag aaatcctgac gctgccttct gtcaatgaaa tggctgaact gttcgatatc
     5161 atagacccga ttgctattgc cgaagtacgc gaagcactca ctcgtactct ggcgactgaa
     5221 ctggcggatg agctgctggc tatttacaac gcgaattacc agagcgagta ccgtgttgag
     5281 catgaagata ttgcaaaacg cactctgcgt aatgcctgcc tgcgcttcct tgcttttggt
     5341 gaaacgcatc tggctgacgt gctggtgagc aagcaatacc atgaagcaaa caatatgacc
     5401 gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc tgccttgccg tgacgcgctg
     5461 atgcaggagt acgacgataa gtggcatcag gacggtctgg tgatggataa atggtttatc
     5521 ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg tgcgtggtct gttgcagcat
     5581 cgctcattta ccatgagcaa cccgaaccgt attcgttcgt tgattggtgc gtttgcgggc
     5641 agcaacccgg cagcgttcca tgccgaagat ggcagcggtt accagttcct ggtggaaatg
     5701 cttaccgacc tcaacagccg taacccgcag gtagcttcac gtctgattga accgctgatt
     5761 cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc gcgcggcgct ggaacagttg
     5821 aaagggctgg aaaatctctc tggcgatctt tacgagaaga taactaaagc actggcttga
     5881 tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaactcc
     5941 gttgaaattg ctggttttgt aggctggata aggcgttcac gccgcatccg gcagccgtgc
     6001 ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc tacaagtccg ttgaaaatgc
     6061 tggttttgtg cgccacatcc ggcatattaa ccatgacgcc gaatcagttg ttcagaatgc
     6121 ccacgccgca ttacccgttg caatacttcc gcttccagct ccgctaatcg caccgaccct
     6181 aatcgacgcg gacgggggat atccactgtc aaatccagac taatttttcc ctcttcaatt
     6241 aacaacaccc ggtcagccat tgctaccgct tcgctcacat catgcgtcac caacagtacg
     6301 gtaaagccgt gctgctgcca aagtgacaca atcaaatcct gcatctcgag tcgcgttaag
     6361 gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac ccggtcgatg aatcaacgct
     6421 cttgccagcg ccacacgctg tttctgcccg ccagaaagtg cggcaggcca ttccccggcg
     6481 cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg catcgcgcca ctgaccttta
     6541 aggcctaacc caacgttatc aatcaccgat ttccacggca gcagacgcgc atcctgaaac
     6601 atcattcgcg tatcatcctg aatttcagcc agcggtgtgg tgcccgctaa cacatcgcct
     6661 gcggttggcg tttccagacc ggccagcagg tgcagcaggg tacttttacc accaccgctg
     6721 cggcccacca ccgccacaaa ctgacctgcc ggaatatgta aatccagttg gttcaggacg
     6781 atattttccg cgtaatgttt gcttactgca ttgagcaaca atggtgtgcc ctggttcaga
     6841 cgagcagtat tcatatcgtg gcctccttca aatgataagc cgggttccag cgcaaccaga
     6901 ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc gagcagggcg taaagaataa
     6961 tagcgactac caccacgtct gtttgcaaaa actctcgcgc attcatcgcc agataaccaa
     7021 tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt cagccacatc aggcccaacg
     7081 caaaacgcac gccgaccata attgagggca gggcatcggg caggatcaca tggataaaca
     7141 gcggtatgcc ggataatcca tagctacgcg ccatctccac cagcccgcga tcgatattac
     7201 ggatgccatg ccaggtgttg atataaatgg ggaacagcgt accgagcgcc accagaaaga
     7261 ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag cgggatcagc gccagatgcg
     7321 gcacgttgcg caacatctga attgaggtat ccagcagcct ttctccccag cgcgacagtc
     7381 cgctaatcag tcccagaatc aggcccagcg atccgccaat tgaaaagcca atcagcgccc
     7441 gccaggaact gatcgccaga tgct
//
LOCUS       DAZPFP010000176         7464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPFP010000176 DAZPFP010000000
VERSION     DAZPFP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.177"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUR4_003395"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUR4_003395"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1456838.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUR4_003396"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUR4_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1456839.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="ACHUR4_003397"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="ACHUR4_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1456840.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="ACHUR4_003398"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="ACHUR4_003398"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1456841.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="ACHUR4_003399"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="ACHUR4_003399"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1456842.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="ACHUR4_003400"
     CDS             complement(5841..6881)
                     /locus_tag="ACHUR4_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1456843.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="ACHUR4_003401"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="ACHUR4_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1456844.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         1771 a         1938 c         1836 g         1919 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaa
//
LOCUS       DAZPFP010000177         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPFP010000177 DAZPFP010000000
VERSION     DAZPFP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.179"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUR4_003402"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUR4_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR1456845.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHUR4_003403"
     CDS             complement(1433..1759)
                     /locus_tag="ACHUR4_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR1456846.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHUR4_003404"
     CDS             1894..2235
                     /locus_tag="ACHUR4_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR1456847.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHUR4_003405"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHUR4_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHUR4_003406"
     CDS             complement(2833..3543)
                     /locus_tag="ACHUR4_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR1456848.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHUR4_003407"
     CDS             3651..3956
                     /locus_tag="ACHUR4_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR1456849.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHUR4_003408"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHUR4_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHUR4_003409"
     CDS             complement(6298..7287)
                     /locus_tag="ACHUR4_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456850.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZPFP010000178         7316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPFP010000178 DAZPFP010000000
VERSION     DAZPFP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.180"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..359)
                     /locus_tag="ACHUR4_003410"
     CDS             complement(<1..359)
                     /locus_tag="ACHUR4_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1456851.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVV"
     gene            569..1582
                     /gene="intI1"
                     /locus_tag="ACHUR4_003411"
     CDS             569..1582
                     /gene="intI1"
                     /locus_tag="ACHUR4_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1456852.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1785..2135
                     /locus_tag="ACHUR4_003412"
                     /pseudo
     CDS             1785..2135
                     /locus_tag="ACHUR4_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            2261..2821
                     /locus_tag="ACHUR4_003413"
     CDS             2261..2821
                     /locus_tag="ACHUR4_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1456853.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            2824..5790
                     /locus_tag="ACHUR4_003414"
     CDS             2824..5790
                     /locus_tag="ACHUR4_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1456854.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            5857..6234
                     /locus_tag="ACHUR4_003415"
     CDS             5857..6234
                     /locus_tag="ACHUR4_003415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1456855.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(6435..7094)
                     /locus_tag="ACHUR4_003416"
     CDS             complement(6435..7094)
                     /locus_tag="ACHUR4_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1456856.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1611 a         2137 c         2019 g         1549 t
ORIGIN      
        1 acaacagaaa attgcatcca cgtctttggt gtatgattgc tgttccagat ctccattcct
       61 ttgggggttt tatcccattt gaatatggtt ttctgatcta ttatttccgc atcaaatgcc
      121 ataagtgata atgcgatctt gaaagttgaa tctggtgcca tttgcgttgc acactttgct
      181 ttattgaatt gagcaatttc agcgtttgtg gatgcatcgt aaagtaaaaa acaaccttca
      241 gttccttcaa ataatggaga tgcaacagta gagatatctg ttgatgcact ggcgctgctg
      301 tagataatat ttgcaattat taaaaaaata gcgaagttga tatgtattgt gtttttcata
      361 ataagtattg gtttggtaaa gggcttaatt ttaacggcta acaattaatg aggctccggg
      421 ttcgcccaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
      481 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
      541 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      601 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
      661 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg
      721 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
      781 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
      841 caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg
      901 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
      961 cgagcatcgt ttgttcgccc agcttctgta tggaacgggc atgcggatca gtgagggttt
     1021 gcaactgcgg gtcaaggatc tggatttcga tcacggcacg atcatcgtgc gggagggcaa
     1081 gggctccaag gatcgggcct tgatgttacc cgagagcttg gcacccagcc tgcgcgagca
     1141 gctgtcgcgt gcacgggcat ggtggctgaa ggaccaggcc gagggccgca gcggcgttgc
     1201 gcttcccgac gcccttgagc ggaagtatcc gcgcgccggg cattcctggc cgtggttctg
     1261 ggtttttgcg cagcacacgc attcgaccga tccacggagc ggtgtcgtgc gtcgccatca
     1321 catgtatgac cagacctttc agcgcgcctt caaacgtgcc gtagaacaag caggcatcac
     1381 gaagcccgcc acaccgcaca ccctccgcca ctcgttcgcg acggccttgc tccgcagcgg
     1441 ttacgacatt cgaaccgtgc aggatctgct cggccattcc gacgtctcta cgacgatgat
     1501 ttacacgcat gtgctgaaag ttggcggtgc cggagtgcgc tcaccgcttg atgcgctgcc
     1561 gcccctcact agtgagaggt agggcagcgc aagtcaatcc tggcggattc actacccctg
     1621 cgcgaaggcc atcggtgccg catcgaacgg ccggttgcgg aaagtcctcc ctgcgtccgc
     1681 tgatggccgg cagcagcccg tcgttgcctg atggatccaa cccctccgct gctatagtgc
     1741 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacaatggag gtggtagccg
     1801 agggtgtgga aacacccgac tgccttgcgt ggttgcggca ggcgggttgc gacacggtgc
     1861 agggtttcct gttcgccagg ccgatgccgg cggcggcctt cgtcggcttc gtcaaccaat
     1921 ggaggaacac caccatgaac gccaatgaac cgagcaccag ttgctgcgtg tgctgcaagg
     1981 aaatcccgct cgatgccgcc ttcacgccgg aaggggccga gtacgtggag catttctgcg
     2041 ggctggagtg ctatcagcgc ttccaggcgc gggccagcac tgcgaccgaa accagcgtca
     2101 aaccggacgc ttgtgattcg ccgccgtcag gttgaggcat accctaacct gatgtcagat
     2161 gccatgtgta aattgcgtca ggataggatt gaattttgaa tttattgaca tatctcgttg
     2221 aaggtcatag agtcttccct gacattttgc agggaattcc atgactggac agcgcattgg
     2281 gtatatcagg gtcagcacct tcgaccagaa cccggaacgg caactggaag gcgtcaaggt
     2341 tgatcgcgct tttagcgaca aggcatccgg caaggatgtc aagcgtccgc aactggaagc
     2401 gctgataagc ttcgcccgca ccggcgacac cgtggtggtg catagcatgg atcgcctggc
     2461 gcgcaatctc gatgatttgc gccggatcgt gcaaacgctg acacaacgcg gcgtgcatat
     2521 cgaattcgtc aaggaacacc tcagttttac tggcgaagac tctccgatgg cgaacctgat
     2581 gctctcggtg atgggcgcgt tcgccgagtt cgagcgcgcc ctgatccgcg agcgtcagcg
     2641 cgagggtatt gcgctcgcca agcaacgcgg ggcttaccgt ggcaggaaga aatccctgtc
     2701 gtctgagcgt attgccgaac tgcgccaacg tgtcgaggct ggcgagcaaa agaccaagct
     2761 tgctcgtgaa ttcggaatca gtcgcgaaac cctgtatcaa tacttgagaa cggatcagta
     2821 aatatgccac gtcgttccat cctgtccgcc gccgagcggg aaagcctgct ggcgttgccg
     2881 gactccaagg acgacctgat ccgacattac acattcaacg ataccgacct ctcgatcatc
     2941 cgacagcggc gcgggccagc caatcggctg ggcttcgcgg tgcagctctg ttacctgcgc
     3001 tttcccggcg tcatcctggg cgtcgatgaa ctaccgttcc cgcccttgtt gaagctggtc
     3061 gccgaccagc tcaaggtcgg cgtcgaaagc tggaacgagt acggccagcg ggagcagacc
     3121 cggcgcgagc acctgagcga gctgcaaacc gtgttcggtt tccggccctt caccatgagc
     3181 cattaccggc aggccgtcca gatgctgacc gagctggcga tgcaaaccga caaaggcatc
     3241 gtgctggcca gcgccttgat cgggcacctg cggcggcagt cggtcattct gcccgccctc
     3301 aacgccgtcg agcgggcgag tgccgaggcg atcacccgtg ctaaccggcg catctacgac
     3361 gccttggccg aaccactggc ggacgcgcat cgccgccgcc tcgacgatct gctcaagcgc
     3421 cgggacaacg gcaagacgac ctggttggct tggttgcgcc agtctccggc caagccaaat
     3481 tcgcggcata tgctggaaca catcgaacgc ctcaaggcat ggcaggcact cgatctgcct
     3541 accggcatcg agcggctggt tcaccagaac cgcctgctca agattgcccg cgagggcggc
     3601 cagatgacac ccgccgacct ggccaaattc gagccgcaac ggcgctacgc cactctcgtg
     3661 gcgctggcca ccgagggcat ggccaccgtc accgacgaaa tcatcgacct gcacgaccgc
     3721 atcctgggta agctgtttaa cgctgccaag aataagcatc agcagcagtt ccaggcgtca
     3781 ggcaaggcca tcaacgccaa ggtacgtctg tacgggcgca tcggtcaggc gctgatcgac
     3841 gccaagcaat caggccgcga tgcgtttgcc gccatcgagg ccgtcatgtc ctgggattcc
     3901 tttgccgaga gcgtcaccga ggcgcagaag ctcgcgcaac ccgatgactt cgatttcctg
     3961 catcgcatcg gcgagagcta cgccaccctg cgccgctatg caccggaatt ccttgccgtg
     4021 ctcaagctgc gggccgcgcc cgccgccaaa aacgtgcttg atgccattga ggtgctgcgc
     4081 ggcatgaaca ccgacaacgc ccgcaagctg ccagccgatg caccgaccgg cttcatcaag
     4141 ccgcgctggc agaaactggt gatgaccgac gccggcatcg accggcgcta ctacgaactg
     4201 tgcgcgctgt ccgagttgaa gaactccctg cgctcgggcg acatctgggt gcagggttca
     4261 cgccagttca aggacttcga ggactacctg gtaccgcccg agaagttcac cagcctcaag
     4321 cagtccagcg aattgccgct ggccgtggcc accgactgcg aacaatatct gcatgagcgg
     4381 ctgacgctgc tggaagcaca acttgccacc gtcaaccgca tggcggcagc caacgacctg
     4441 ccggatgcca tcatcaccga gtcgggcttg aagatcacgc cgctggatgc ggcggtgccc
     4501 gacaccgcgc aggcgctgat agaccagaca gccatggtcc tgccgcacgt caagatcacc
     4561 gaactgctgc tcgaagtcga tgagtggacg ggcttcaccc ggcacttcac gcacttgaaa
     4621 tcgggcgatc tggccaagga caagaacctg ttgttgacca cgatcctggc cgacgcgatc
     4681 aacctgggcc tgaccaagat ggccgagtcc tgccccggca cgacctacgc gaagctcgct
     4741 tggctgcaag cctggcatac ccgcgacgaa acgtactcga cagcgttggc tgaactggtc
     4801 aacgctcagt ttcggcatcc ctttgccggg cactggggcg atggcaccac atcatcatcg
     4861 gacggacaga atttccgaac cgctagcaag gcaaagagca cggggcacat caacccaaaa
     4921 tatggcagca gcccaggacg gactttctac acccacatct ccgaccaata cgcgccattc
     4981 cacaccaagg tggtcaatgt cggcctgcgc gactcaacct acgtgctcga cggcctgctg
     5041 taccacgaat ccgacctgcg gatcgaggag cactacaccg acacggcggg cttcaccgat
     5101 cacgtcttcg ccctgatgca cctcttgggc ttccgcttcg cgccgcgcat ccgcgacctg
     5161 ggcgacacca agctctacat cccgaagggc gatgccgcct atgacgcgct caagccgatg
     5221 atcggcggca cgctcaacat caagcacgtc cgcgcccatt gggacgaaat cctgcggctg
     5281 gccacctcga tcaagcaggg cacggtgacg gcctcgctga tgctcaggaa actcggcagc
     5341 tacccgcgcc agaacggctt ggccgtcgcg ctgcgcgagt tgggccgcat cgagcgcacg
     5401 ctgttcatcc tcgactggct gcaaagcgtc gagctacgcc gccgcgtgca tgccgggctg
     5461 aacaagggcg aggcgcgcaa tgcgctggcc cgtgccgtgt tcttcaaccg ccttggtgaa
     5521 atccgtgacc gcagtttcga gcagcagcgc taccgggcca gcggcctcaa cctggtgacg
     5581 gcggccatcg tgctgtggaa cacggtctac ctggagcgtg cggcgcatgc gttgcgcggc
     5641 aatggtcatg ccgtcgatga ctcgctattg cagtacctgt cgccactcgg ctgggagcac
     5701 atcaacctga ccggtgatta cctatggcgc agcagcgcca agatcggcgc ggggaagttc
     5761 aggccgctac ggcctctgca accggcttag cgtgctttat tttccgtttt ctgagacgac
     5821 ccctattatg gcgaaggtat cattcaggaa gcgttgatga aggattatct ctgtaaaaag
     5881 ttattcaacc ggctttccgg taccctggtg atcagagcgc gctgcggcaa taacattact
     5941 ggcctggcat gttgcaatat tctttatccc tcgccccgat acagcggtca gctgcatatt
     6001 aaagagctgt atgtttctca gtgcgatcga aataagggca cgggtaaagc gataatgcgc
     6061 tttatagccc ggcttgcgct tgaacaggaa tgccttagcc ttagctggaa cgctgaaaaa
     6121 tccaaccccg gcgctaaccg tttttatcag gctctgggag gcagaataaa tgatcatatc
     6181 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
     6241 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     6301 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     6361 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     6421 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     6481 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     6541 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     6601 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     6661 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     6721 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     6781 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     6841 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     6901 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     6961 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     7021 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     7081 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     7141 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     7201 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     7261 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtc
//
LOCUS       DAZPFP010000179         7248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPFP010000179 DAZPFP010000000
VERSION     DAZPFP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.181"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="ACHUR4_003417"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="ACHUR4_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR1456857.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="ACHUR4_003418"
     CDS             1103..2563
                     /locus_tag="ACHUR4_003418"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1456858.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="ACHUR4_003419"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="ACHUR4_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1456859.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="rspR"
                     /locus_tag="ACHUR4_003420"
                     /gene_synonym="ydfH"
     CDS             complement(2980..3666)
                     /gene="rspR"
                     /locus_tag="ACHUR4_003420"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1456860.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="ACHUR4_003421"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="ACHUR4_003421"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1456861.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="ACHUR4_003422"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="ACHUR4_003422"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1456862.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="ACHUR4_003423"
     CDS             complement(6728..7246)
                     /locus_tag="ACHUR4_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1456863.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1787 a         1720 c         1865 g         1876 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctggcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactg
//
LOCUS       DAZPFP010000180         7244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPFP010000180 DAZPFP010000000
VERSION     DAZPFP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.182"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUR4_003424"
     CDS             complement(<1..190)
                     /locus_tag="ACHUR4_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1456864.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHUR4_003425"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHUR4_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1456865.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHUR4_003426"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHUR4_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1456866.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHUR4_003427"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHUR4_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1456867.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHUR4_003428"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHUR4_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHUR4_003429"
     CDS             complement(3262..3381)
                     /locus_tag="ACHUR4_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1456868.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUR4_003430"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUR4_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1456869.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUR4_003431"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUR4_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1456870.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHUR4_003432"
     CDS             3803..4147
                     /locus_tag="ACHUR4_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1456871.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHUR4_003433"
     CDS             4151..4486
                     /locus_tag="ACHUR4_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1456872.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHUR4_003434"
     CDS             complement(4543..6864)
                     /locus_tag="ACHUR4_003434"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1456873.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHUR4_003435"
     CDS             complement(6981..7190)
                     /locus_tag="ACHUR4_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456874.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1966 a         1721 c         1613 g         1944 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatg
//
LOCUS       DAZPFP010000181         7238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPFP010000181 DAZPFP010000000
VERSION     DAZPFP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.183"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            246..1817
                     /gene="garD"
                     /locus_tag="ACHUR4_003436"
     CDS             246..1817
                     /gene="garD"
                     /locus_tag="ACHUR4_003436"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1456875.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1869..2693)
                     /gene="agaR"
                     /locus_tag="ACHUR4_003437"
     CDS             complement(1869..2693)
                     /gene="agaR"
                     /locus_tag="ACHUR4_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1456876.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2942..4222
                     /gene="kbaZ"
                     /locus_tag="ACHUR4_003438"
     CDS             2942..4222
                     /gene="kbaZ"
                     /locus_tag="ACHUR4_003438"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1456877.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNVRRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4245..4718
                     /gene="agaV"
                     /locus_tag="ACHUR4_003439"
     CDS             4245..4718
                     /gene="agaV"
                     /locus_tag="ACHUR4_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1456878.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4729..5508
                     /gene="agaW"
                     /locus_tag="ACHUR4_003440"
     CDS             4729..5508
                     /gene="agaW"
                     /locus_tag="ACHUR4_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1456879.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5498..6307
                     /gene="agaE"
                     /locus_tag="ACHUR4_003441"
     CDS             5498..6307
                     /gene="agaE"
                     /locus_tag="ACHUR4_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1456880.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6394..6828
                     /gene="agaF"
                     /locus_tag="ACHUR4_003442"
     CDS             6394..6828
                     /gene="agaF"
                     /locus_tag="ACHUR4_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1456881.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6825..>7238
                     /locus_tag="ACHUR4_003443"
     CDS             6825..>7238
                     /locus_tag="ACHUR4_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1456882.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1721 a         1872 c         1917 g         1728 t
ORIGIN      
        1 tgcaaatgct aatttaatta atacttttta aatattattt tgagcatatg cacataaggt
       61 tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctaaaat
      121 gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa
      181 atcatttatt tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt
      241 gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc
      301 acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt
      361 ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg
      421 acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg
      481 caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt
      541 tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca
      601 cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca
      661 ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg
      721 atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt
      781 gtggcgtggc gattaacgca ccggcagcag ttgtacctat tcgtaccatt cacaatattt
      841 cgctgaaccc taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc
      901 agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta gaaagcgcca
      961 gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt
     1021 tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg
     1081 cttcagaact ggttgtcggc atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg
     1141 caaacccggc ggttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt
     1201 tttcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg
     1261 aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg
     1321 gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctagcaaacg
     1381 tggtagagaa agcgctcggc tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc
     1441 tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg
     1501 attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg
     1561 gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggca ccccgcaccg
     1621 agctggcgaa ccgctggttt gatttaatgg atattaatgc gggcaccatc gctaccggcg
     1681 aagaaactat tgaagaggag ggctggaagt tgttccactt tattctcgac gtcgccagcg
     1741 ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc
     1801 cagcaccggt gacctgataa ttcaattcgt attatgcccg atagcacacg tttatcgggc
     1861 atattgagtc acagcgaaga cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt
     1921 cgcagtcctt ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt
     1981 tgagtatcaa tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg
     2041 atgatccgtt ccgcaacttc gcacatccga cggcttaaac gggcttcatc ttcattatgt
     2101 gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg
     2161 taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa
     2221 tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc
     2281 ataccgttgg tcatcgcaat tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa
     2341 gtggtggtcc cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct
     2401 ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac tgatggctcg
     2461 actgacggcg tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc
     2521 tgcttttcca gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc
     2581 gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt
     2641 cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt
     2701 cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa
     2761 tgcaagccca ccttgcccat tggcgcaagc tactctcgtt tcagtgactt tcattacgtt
     2821 tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac
     2881 gcagtatcaa ttgaaacaaa acgaaagatc aatatcgcag taatctgaac tggagaggaa
     2941 agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta
     3001 tgccgtttgt tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa
     3061 ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac
     3121 cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt
     3181 cccgcaggat gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct
     3241 gccagccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg
     3301 attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccaa ttcccttaac
     3361 tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg
     3421 cgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg
     3481 cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac
     3541 gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat
     3601 tattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca
     3661 gcccgccaaa gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga
     3721 agcgcactct accgattatc aaacgccgca atctctgcgc cagctggtga ttgaccactt
     3781 tgccattctg aaagttggcc cggcgctgac cttcgccctg cgtgaagccc ttttttctct
     3841 ggcggcgatt gaagaagaac tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct
     3901 ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa
     3961 cgtgcgtcgt ctggcgcgtg gttacagcta ctcagatcgc gtgcgctatt actggccgga
     4021 cagccagatt gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc
     4081 gctaccgctc atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct
     4141 gcaaccgacg ccacgggaac tcattataaa ccatattcag gacatcctgg cgcagtacca
     4201 cacagcctgt gaaggccaat aagcaaaaca aagaggaaca tgctatgcca aatattgttt
     4261 taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg
     4321 cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa
     4381 acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag
     4441 ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac
     4501 ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca
     4561 atatgcacta cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg
     4621 atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc
     4681 cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagc
     4741 ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctaga tatgtttaac
     4801 ggcttaaccc atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc
     4861 gatctgcata ccggaatttt aaccggcgat acgctggaac tagtgtggat ggggctggcc
     4921 ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt
     4981 gccattacta ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc
     5041 gcagtacaga tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc
     5101 gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg
     5161 ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa
     5221 catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg
     5281 ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc
     5341 tacataccct acttcatcct gggctttgtt gccgcaacct ggctcaagtt accggtgctg
     5401 gctattgctg ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa
     5461 ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct
     5521 gccgaacgtc agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga
     5581 agatcggagc attgccgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc
     5641 catgctgtta caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg
     5701 tctgctgcct gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa
     5761 ggggcatatg ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat
     5821 ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt
     5881 cggtgcgccg cttggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg
     5941 tggcgggata ggcgcaagcc tcgcgctaca aggctctatt cttggcgcag tcgtctttat
     6001 tgtgctgttc aacgtggtac acttggggct gcgttttggt ctggcgcatt atgcttaccg
     6061 catgggcgtg gcggcgattc cccttattaa agcgaatacc aaaaaagtcg gccatgctgc
     6121 gtcaatcgtt ggaatgacgg taatcggcgc actggtggca acctatgttc gtttaagcac
     6181 cacgctggaa atcacggcgg gcgacgcagt agttaagtta caggctgatg ttatcgacaa
     6241 actgatgcct gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg
     6301 cggctagagt ccgctgcgcc tgattgcggt taccgtggtt ctagggatcg tcggtaaatt
     6361 ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat
     6421 ggcgggtttg ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag
     6481 tttattgcca tcgattttcc cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa
     6541 gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc
     6601 ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt
     6661 accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt
     6721 gaagaattcc gtgtacaggc gctggagtgt ggacaccgag ggctgaccag tctggtggac
     6781 gagttaggcc gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc
     6841 gcgccagaag gctgctgact gaagagggat ggttcgatga ccatcagttg cgtgttgctg
     6901 acggtgtcat cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac
     6961 ggctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg
     7021 atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca
     7081 gttggttacc gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctgaaacgta
     7141 ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag
     7201 gaccgtactt cacgccgcag aataaaggcg cgcatccg
//
LOCUS       DAZPFP010000182         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPFP010000182 DAZPFP010000000
VERSION     DAZPFP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.184"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHUR4_003444"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHUR4_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUR4_003445"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUR4_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR1456883.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUR4_003446"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUR4_003446"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR1456884.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUR4_003447"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUR4_003447"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR1456885.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUR4_003448"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUR4_003448"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR1456886.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUR4_003449"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUR4_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHUR4_003450"
     CDS             7090..>7183
                     /locus_tag="ACHUR4_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR1456887.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZPFP010000183         7182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPFP010000183 DAZPFP010000000
VERSION     DAZPFP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.185"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(94..2097)
                     /locus_tag="ACHUR4_003451"
     CDS             complement(94..2097)
                     /locus_tag="ACHUR4_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456888.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2094..3230)
                     /locus_tag="ACHUR4_003452"
     CDS             complement(2094..3230)
                     /locus_tag="ACHUR4_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1456889.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3223..5502)
                     /locus_tag="ACHUR4_003453"
     CDS             complement(3223..5502)
                     /locus_tag="ACHUR4_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR1456890.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5513..6601)
                     /locus_tag="ACHUR4_003454"
     CDS             complement(5513..6601)
                     /locus_tag="ACHUR4_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR1456891.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1679 a         1931 c         1917 g         1655 t
ORIGIN      
        1 ggtgactctc accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga
       61 actggagtag aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa
      121 taattgttcg caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt
      181 attacgaaat tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt
      241 cgccagcaac tctgcctgat taatagcgct ctgttcctga tagcgccagc cgttatggag
      301 cagttgaata agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg
      361 cagaacgctg gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca
      421 ggcacgagtc tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact
      481 gacaaagcca gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg
      541 ttcaatggca taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc
      601 accttcgccg ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga
      661 aatacactca gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca
      721 ggcgggggga cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg
      781 cgcgctcaac atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg
      841 ggtagctaac agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc
      901 acccgccagt gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc
      961 ctgttcactt cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt
     1021 aaaccagatg cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac
     1081 cagccgcaga tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca
     1141 cggcacagga ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa
     1201 tcgactgttt aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg
     1261 ggcgtggaag gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca
     1321 gctgcagcgt tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg
     1381 ctggctgatg ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt
     1441 tttgccttct tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg
     1501 ccagattaaa tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc
     1561 cagtacgaca tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact
     1621 gctgcgggaa taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg
     1681 cgcacagaac aactcaatgg tgatgctttt agcgaccagc gcctgtgcgc gtttgcgggt
     1741 ggcatcggga agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt
     1801 tcccgtaggc tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg
     1861 acggcacatg ccgctggccc cataggtgca ctgagcctct ttcagtgcct ggccgttcgc
     1921 cagctgggta cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat
     1981 ttccgggacg ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct
     2041 taacgccgtc agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac
     2101 tgaagattct ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg
     2161 ccgacattaa ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg
     2221 agcgctgcca gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc
     2281 agcaatgatg atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca
     2341 ggttgttcaa taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct
     2401 aattgcactt tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg
     2461 cttgtgtcaa acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc
     2521 atcacagcag agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc
     2581 agttgccatt gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac
     2641 aacttgccgt gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca
     2701 aagtttcgcg ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc
     2761 tgacgaactt cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg
     2821 cgggcggcag ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc
     2881 aggctttcgg aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg
     2941 ccgtagcgca gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg
     3001 tgaatactgt tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt
     3061 tcaccacgct ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc
     3121 tgccgggcgt tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat
     3181 cgccagcgtt gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc
     3241 ccagtgttgc agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg
     3301 cggtggacaa tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg
     3361 ctcaagcacc tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc
     3421 gcgtaaatcg ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct
     3481 gaaaccggcg ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc
     3541 gtgcagcgcc tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc
     3601 ggcactagcg ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag
     3661 tgccaggaca tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga
     3721 aacgccgggg agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca
     3781 gctatgcaag tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca
     3841 cagcgtgcga tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc
     3901 gctaatttca tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc
     3961 aaattgattt tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga
     4021 cgccagaccc gctgacgccg cttggctaag acatgtggta atgctgccga ttccacccgc
     4081 gtccagcata tcggcttcta atttatggcg tgcggcttcc aggagtgtgg caccaaagca
     4141 ggcggcctca atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc
     4201 accgttgcct gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag
     4261 tattgccagc cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa
     4321 ggtaagctcc gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg
     4381 cagaggcggt tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc
     4441 tatcaacgtt agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg
     4501 caacggcgca ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt
     4561 gcgtaacggt agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc
     4621 agccatatcc gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt
     4681 tagcagttgt tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg
     4741 ggcaggcatt cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggt
     4801 gagataacaa cctgtaacgg catctgccag cgacggcaat tctggcgtgt taattacctg
     4861 cggacattcg cgccacattt ttgccagtgc cggagcgtgc cagccaccgc agacaactaa
     4921 cacgtcgcca ttattttgct gcatcgccca tccaatccag cgggccataa aggcttcgcg
     4981 ctgacgattt agagcatcgc cgggggagtc gccccgcagt tgggcaaaat agtgcgccag
     5041 tgcagagggt aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtgtc
     5101 gctgttatcc atgcgggtgg cacgcagcag caaggtctgg ctttcctctt gcgtatcagg
     5161 cgaatcgtcc tcttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc
     5221 ctgaatacga cgatcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt
     5281 ccaggcacca cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac
     5341 cggaagctgg tgggctaaaa acagttcgtc tacccggtca ttaaaatcag cagggccttc
     5401 aatcaacacg tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc
     5461 cggactatga tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca
     5521 ggcggtggcg ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgacacgct
     5581 gttcgaagta acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg
     5641 caatgcagtc caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga
     5701 cgcctacggc gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat
     5761 ccattgacgt tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgg accagtaact
     5821 ccagtaccgc gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag
     5881 cactggcgac aagctccagc tcctgggcga aatccataat cggaaagaca gtttcaaaat
     5941 cgaagcgacg ctttagcgca gcactcattt cattgacgcc gcgatcgcgg gtattggcag
     6001 tggcaataat attaaagcct tctcgcgcat agagctgact ggcttcacca gtgagttccg
     6061 gcaccgtcat caccctgtca gaaagcatac ctaacagaca gtcttgcact tccagcggtg
     6121 tacgcgttat ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg
     6181 cagggaccag cgcttccgtt gatggaccat gattgataag cagggcgtag ttccagccgt
     6241 acttgatttg atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc
     6301 taatagaggt tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca
     6361 acatcaaacc gcgtccggtg gcaagagtga ctaacatacg ttcgacagcg gagggactaa
     6421 tcaccacttt acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag
     6481 gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc attttgtttt aattttgcca
     6541 gttcatcggc gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca
     6601 tatattcatc cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt
     6661 ggcgcattag ttgtttgagc gaaaaatatc tggaaagcag atcacagtaa ataaaaaaaa
     6721 ctgctggtga ggcaggttct tttatttact attttcaggc ctaacaccac tgcctgtgca
     6781 acatcgtgtt ccaacgaacg aattataacc acctctggac agtgttgcaa ttaagataat
     6841 gagatttata atcctgactg ttgggaagtt gcgccggatc gaacctattt caagtcctgg
     6901 ctaccggacg ggtggtgccg caggtagtgc tctgatccgg agtgcaacat ggcgactcaa
     6961 caaacgtaaa ccagtatcga caggcagtgt aagggtaagg tgctggtgtt gtgctcctta
     7021 ataaggagag cggatggaca cgctgacaca aaagttaacc gtgctcattg ctgtactgga
     7081 gctattagtg gctctgttac ggttgattga tttgttgaag taacgggcaa attgaaacgc
     7141 atctaagggg gaataccgtt ccccccttta ataaagctat ac
//
LOCUS       DAZPFP010000184         7140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPFP010000184 DAZPFP010000000
VERSION     DAZPFP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.186"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..152)
                     /locus_tag="ACHUR4_003455"
     CDS             complement(<1..152)
                     /locus_tag="ACHUR4_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456892.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLN"
     gene            230..1324
                     /gene="ddlA"
                     /locus_tag="ACHUR4_003456"
     CDS             230..1324
                     /gene="ddlA"
                     /locus_tag="ACHUR4_003456"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1456893.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1348..1560)
                     /locus_tag="ACHUR4_003457"
     CDS             complement(1348..1560)
                     /locus_tag="ACHUR4_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1456894.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1820..2128
                     /locus_tag="ACHUR4_003458"
     CDS             1820..2128
                     /locus_tag="ACHUR4_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1456895.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2187..3281)
                     /gene="yaiW"
                     /locus_tag="ACHUR4_003459"
                     /pseudo
     CDS             complement(2187..3281)
                     /gene="yaiW"
                     /locus_tag="ACHUR4_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3294..4514)
                     /gene="sbmA"
                     /locus_tag="ACHUR4_003460"
     CDS             complement(3294..4514)
                     /gene="sbmA"
                     /locus_tag="ACHUR4_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1456896.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4866..6023
                     /gene="ampH"
                     /locus_tag="ACHUR4_003461"
     CDS             4866..6023
                     /gene="ampH"
                     /locus_tag="ACHUR4_003461"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1456897.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6024..6527)
                     /locus_tag="ACHUR4_003462"
     CDS             complement(6024..6527)
                     /locus_tag="ACHUR4_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1456898.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6615..>7140)
                     /locus_tag="ACHUR4_003463"
     CDS             complement(6615..>7140)
                     /locus_tag="ACHUR4_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1456899.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1812 a         1824 c         1726 g         1778 t
ORIGIN      
        1 taattaagag aaaaaaactc accgcattgc catttaagcc aggaataaat tagcttcccg
       61 ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt caccagtacg
      121 ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga atcccccgtt
      181 ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact
      241 gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc
      301 aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga
      361 taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc
      421 tgcccatatt gcgttgcgcc cttcggcgac cagccttgcg caggtgccag gtaaacatga
      481 gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc
      541 gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa
      601 tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgtcac
      661 caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc
      721 taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cactgtttgt
      781 aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta
      841 cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat
      901 caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg
      961 tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga
     1021 tggcgcgaaa gtggtagttc cggcagccat tgcaccagag atcaacgata agatccgggc
     1081 gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt
     1141 tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa
     1201 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     1261 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     1321 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     1381 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     1441 ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa gaatgaatat
     1501 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     1561 ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt
     1621 agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt gtgactcaca
     1681 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     1741 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     1801 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     1861 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     1921 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     1981 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     2041 cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg
     2101 gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc
     2161 ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct
     2221 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     2281 atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa
     2341 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg
     2401 cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt
     2461 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     2521 ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     2581 aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa
     2641 gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt
     2701 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     2761 ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc
     2821 agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     2881 taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg
     2941 tgcatccgtt cagcacgacg gtcaatctct tgctaggcga tcttgcttaa gcccggaacg
     3001 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gtagacgttt
     3061 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     3121 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct
     3181 ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc
     3241 gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc
     3301 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     3361 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     3421 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     3481 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     3541 agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt
     3601 acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca
     3661 taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc
     3721 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     3781 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     3841 gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacgccc
     3901 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct
     3961 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     4021 cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc
     4081 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     4141 gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt gacggcgacc
     4201 ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag tattgaccag
     4261 tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca
     4321 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     4381 atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc ttgccagaag
     4441 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     4501 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     4561 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa
     4621 ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga
     4681 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     4741 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     4801 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac
     4861 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     4921 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     4981 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     5041 accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc
     5101 tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa atgctggtga
     5161 aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat gctccgccag
     5221 gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg gcaacccata
     5281 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     5341 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     5401 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     5461 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg gggatgaaag
     5521 acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     5581 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     5641 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     5701 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     5761 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     5821 agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg
     5881 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     5941 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     6001 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     6061 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     6121 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     6181 aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt ggctggtaga
     6241 tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa
     6301 ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct
     6361 ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt gacatcactg
     6421 gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat
     6481 ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt
     6541 gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt
     6601 cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct
     6661 acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg
     6721 aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca
     6781 ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga
     6841 gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa
     6901 cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca
     6961 ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt
     7021 ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca
     7081 acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag
//
LOCUS       DAZPFP010000185         7062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPFP010000185 DAZPFP010000000
VERSION     DAZPFP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.187"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..4858
                     /gene="traI"
                     /locus_tag="ACHUR4_003464"
                     /pseudo
     CDS             1..4858
                     /gene="traI"
                     /locus_tag="ACHUR4_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            4878..5624
                     /gene="traX"
                     /locus_tag="ACHUR4_003465"
     CDS             4878..5624
                     /gene="traX"
                     /locus_tag="ACHUR4_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1456900.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5679..6239
                     /locus_tag="ACHUR4_003466"
     CDS             5679..6239
                     /locus_tag="ACHUR4_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1456901.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6370..6582
                     /locus_tag="ACHUR4_003467"
     CDS             6370..6582
                     /locus_tag="ACHUR4_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1456902.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            6826..>7062
                     /locus_tag="ACHUR4_003468"
     CDS             6826..>7062
                     /locus_tag="ACHUR4_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1456903.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1630 a         1771 c         2313 g         1348 t
ORIGIN      
        1 tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat caggaaccac
       61 agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa tggaagacac
      121 tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct aatcagattg
      181 cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg ggctatgaaa
      241 ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg gaggcctttt
      301 ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg aaatcccggg
      361 atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa gtcagaatgg
      421 ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat cgtgacgcag
      481 cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag gacgggccgg
      541 atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa gtgcagttta
      601 tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat ggtgtgattg
      661 aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc cccctcgatc
      721 gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca gtccgggcac
      781 tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa agtgtccccc
      841 ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg tcgctggcca
      901 ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa ctggtcatga
      961 tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc tcgcagatga
     1021 acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt cagttgctgg
     1081 aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa aaactctccc
     1141 tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag gtcctgataa
     1201 ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag gatgccgggg
     1261 tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc agtgaaccgg
     1321 accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag aagagagcgt
     1381 ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc gcagtgagct
     1441 gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt caccggtctg
     1501 gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg tgatggagca
     1561 gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga cggcgcagag
     1621 tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta tttcttccct
     1681 ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg gtgagcgtct
     1741 gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc tgcaggtggc
     1801 atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac cggccaccct
     1861 gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg tggaaacgcc
     1921 cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga tggcaatgga
     1981 caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt attcctcact
     2041 ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg tggtttctga
     2101 gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc tgcagaaagc
     2161 cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc tggaaagtaa
     2221 aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg ctgcagaagg
     2281 aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg gtgatttact
     2341 gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg cgtcgtatga
     2401 ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca cgccgctgat
     2461 ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg ccgccacccg
     2521 tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg gtgtgggtaa
     2581 gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga gtgaacgtcc
     2641 ccgtgtcgtg gggctggtgc ccacacaccg tgcggtcggg gagatgcgca gcgccggcgt
     2701 ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc gcagcggaga
     2761 aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg tgggcaatac
     2821 cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg tggccagcgg
     2881 tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc agcagacgcg
     2941 cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg aactgcggga
     3001 ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc ttgagcgtgt
     3061 gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact ccgtgacgga
     3121 gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga tgctgaaagg
     3181 cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg actataccgg
     3241 caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg aggaccggcg
     3301 cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg ggaaggtgca
     3361 ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc gtcgtctctc
     3421 cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc accggattgc
     3481 cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta acacgcggct
     3541 gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg acaccatccg
     3601 ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg gttatgtggc
     3661 caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt ctgacggtca
     3721 gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg acctggccta
     3781 tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg cgcttgaagg
     3841 cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg ccctgtcgcg
     3901 tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg atgccatcaa
     3961 caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg accgggaggt
     4021 catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg cggcaggccg
     4081 tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt ttattgctcc
     4141 gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta acggcaagtc
     4201 cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc ggggattcag
     4261 cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc agggcagccg
     4321 taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg cccgtgataa
     4381 tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga atcccggtgc
     4441 catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc cgggagccgg
     4501 aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag aggccatccg
     4561 ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga acaaacctga
     4621 cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg agcgtgaccg
     4681 ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg aaccacaacg
     4741 ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc gtctgcagca
     4801 gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg gagactgata
     4861 caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac gattcactgg
     4921 ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga cagcgggata
     4981 tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg atattccagc
     5041 tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt gccctggtgt
     5101 gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac cggctgtggg
     5161 gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg tatgagggga
     5221 atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg
     5281 ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg tccggcacca
     5341 gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac cgggcggaag
     5401 acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg gcgcttaacc
     5461 ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg accgttggtc
     5521 tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt ttcccgacgt
     5581 tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg tggtatcttt
     5641 ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag aagcgaccgg
     5701 tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg aaaaccatca
     5761 tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc gagaaagcgg
     5821 cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg tccatttatc
     5881 tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg ccgggattat
     5941 ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta ctggaagacg
     6001 tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg aaggccatca
     6061 cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat gacacggaag
     6121 ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg cgtctggcga
     6181 aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat gagaaataaa
     6241 aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa taacttccgc
     6301 ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca actaaagggg
     6361 gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg gcgttaccag
     6421 cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga agcggcagaa
     6481 gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt tgcccggaaa
     6541 agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa gcagccttca
     6601 ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta atatccggtc
     6661 gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta agtcgatttt
     6721 ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct gcactattca
     6781 ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag gaaatatatt
     6841 ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca tggacgggtt
     6901 gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc ggtgcggatc
     6961 aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg gtcaacagac
     7021 atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZPFP010000186         7060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPFP010000186 DAZPFP010000000
VERSION     DAZPFP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.188"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHUR4_003469"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHUR4_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHUR4_003470"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHUR4_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..>7060
                     /gene="yehK"
                     /locus_tag="ACHUR4_003471"
     CDS             6769..>7060
                     /gene="yehK"
                     /locus_tag="ACHUR4_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFR1456904.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQ"
BASE COUNT         1859 a         1824 c         1713 g         1664 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga
//
LOCUS       DAZPFP010000187         6954 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPFP010000187 DAZPFP010000000
VERSION     DAZPFP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.190"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            18..515
                     /gene="ftnA"
                     /locus_tag="ACHUR4_003472"
     CDS             18..515
                     /gene="ftnA"
                     /locus_tag="ACHUR4_003472"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1456905.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLTGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(552..791)
                     /locus_tag="ACHUR4_003473"
     CDS             complement(552..791)
                     /locus_tag="ACHUR4_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1456906.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            981..2192
                     /gene="tyrP"
                     /locus_tag="ACHUR4_003474"
     CDS             981..2192
                     /gene="tyrP"
                     /locus_tag="ACHUR4_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1456907.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2254..2919)
                     /gene="yecA"
                     /locus_tag="ACHUR4_003475"
     CDS             complement(2254..2919)
                     /gene="yecA"
                     /locus_tag="ACHUR4_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1456908.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3115..3201)
                     /locus_tag="ACHUR4_003476"
     tRNA            complement(3115..3201)
                     /locus_tag="ACHUR4_003476"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3165..3167),aa:Leu,seq:taa)
     gene            complement(3214..3287)
                     /locus_tag="ACHUR4_003477"
     tRNA            complement(3214..3287)
                     /locus_tag="ACHUR4_003477"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3253..3255),aa:Cys,seq:gca)
     gene            complement(3342..3417)
                     /locus_tag="ACHUR4_003478"
     tRNA            complement(3342..3417)
                     /locus_tag="ACHUR4_003478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3382..3384),aa:Gly,seq:gcc)
     gene            complement(3569..4117)
                     /gene="pgsA"
                     /locus_tag="ACHUR4_003479"
     CDS             complement(3569..4117)
                     /gene="pgsA"
                     /locus_tag="ACHUR4_003479"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1456909.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4174..6006)
                     /gene="uvrC"
                     /locus_tag="ACHUR4_003480"
     CDS             complement(4174..6006)
                     /gene="uvrC"
                     /locus_tag="ACHUR4_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1456910.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6003..6659)
                     /gene="uvrY"
                     /locus_tag="ACHUR4_003481"
     CDS             complement(6003..6659)
                     /gene="uvrY"
                     /locus_tag="ACHUR4_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1456911.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6801..6872
                     /locus_tag="ACHUR4_003482"
                     /pseudo
     CDS             6801..6872
                     /locus_tag="ACHUR4_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1690 a         1802 c         1640 g         1822 t
ORIGIN      
        1 ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga
       61 acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata
      121 ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc
      181 agcgtctgtt tgattacctg actgataccg gcaatttacc gcgtattaat accgttgaat
      241 ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac
      301 aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc
      361 caacatttaa tttcctgcag tggtatgttt ctgagcagca tgaagaagag aaactgttca
      421 aatcgattat tgataaatta agcctgacag gcaaaagcgg cgaaggtctg tattttatcg
      481 acaaagaact ctctaccctc gacacacaaa actaatgctc tccggcggca ggtttctgcc
      541 gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa
      601 ggtataaatt tgccttttgc tgccagaaac gctaccagct ctcccgctgt caccccttct
      661 gccgagcacg tgtgaaaacg tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga
      721 ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga
      781 atagagtcca tcgttcctcc tcaaaaaaag cctagcgtag cgattaccgc ttatgaagac
      841 tttgcgccag cgcaggactg aatgctttta ttgtacattt atatttacac aatatgtaac
      901 gtcggtttga cgaagcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga
      961 tcgtcaggac agaagaaagc gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg
     1021 gaaccacaat tggcgcaggc atgctggcaa tgccgctggc tgcggccggt gttggtttta
     1081 gcgttacgtt aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac
     1141 tgctggaggt gtaccagcat gttccggcag ataccggtct gggcacgctg gcaaaacgct
     1201 atctgggacg ctacggtcaa tggctgacgg gcttcagtat gatgttctta atgtatgctc
     1261 tgactgcagc atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga
     1321 caggtatttc aatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg
     1381 tggtttgtgt tggaacatcg ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa
     1441 ttatttttct ggtggtgatg ctggtattgc tgctaccgca tattcacaaa gtgaatcttt
     1501 taaccctgcc gttgcaacag gggctggcac tgtctgcaat cccggtgatt tttacgtcgt
     1561 ttggttttca cggtagcgtg ccgagtattg tcagctatat ggatggcaac attcgtaagc
     1621 tacgctgggt gtttataatc ggtagtgcca tccccctggt ggcatatatt ttctggcagg
     1681 tggcgacgct tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgctg
     1741 gattaaacgg gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg
     1801 cggtgcattt atttgctgat ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct
     1861 tatttgatta tctggctgat ttgtttcagc gttcaaatac cgttggtgga cgattgcaaa
     1921 ctggtgcaat tacctttctg ccgccgttgg cgtttgcact gttttatcca cgaggatttg
     1981 tgatggcgct gggttacgcc ggtgtggcgc tggcggtact ggcattgatt atcccttcac
     2041 tattgacctg gcaaagcaga aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc
     2101 gtccggcgct ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga
     2161 ttgcggcagg gttgttacca gaagtggggt gatcagatag cctcaaattc cttattgggt
     2221 gccagaatta acgctgacac ccaatgtgac ctcttaatgc aggcagcact gcttaaattt
     2281 cttaccactg ccgcacggac aaggatcgtt acgccccggt ttctcttctg ctttgatcgg
     2341 ttgctgaaca gctttttcct gcggatgcgc catccagtat gcatgtaaat caagcgccgc
     2401 cagtcgaatg gcatctacgc tctcttcaaa cgcttctggc gacattttct ctacccgctc
     2461 gaagttttcc tcagtaccgt gcagcgcaat cgcctccagc gctggtttta aagaatcggg
     2521 caacgttgac cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac
     2581 aatcgtcagc tcgctgccat caacttctcg caagccgaat aacggctcaa actgctccgg
     2641 gaattcgttc agacgctctg cggtatcggc catatgttga aaagccaggt tcataaagcg
     2701 cgtcatctct ttctctgacg cccaacgcgg cacatagtca gccccacccc acacggcaac
     2761 cagccactgt tctggttcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc
     2821 cagctccgcc acatcaagga tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag
     2881 ccattccaac tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc
     2941 aactcgccag cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt
     3001 gccggtgatt aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc
     3061 gaaggtggtt tcacgacact gcttattact ttgattttat tcttatcttt cccatggtac
     3121 ccggagcggg acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc
     3181 taccgattcc accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg
     3241 actagacgga tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc
     3301 ctttcagcca gacatccgct tgacgccgat gtcttttaaa ctggagcggg aaacgagact
     3361 cgaactcgag acctcgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt
     3421 catcaagcaa tcagttaatc acttgatttt attatcgtct ggcaatcagt gccgccgttc
     3481 gatgcgttgc attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga
     3541 tcgtttgctg aaaattacgc cgaaacgatc actgatcaag caaatctgca cgcgcagcgc
     3601 tcaaatattg caacattgac cacagagtca gtaccgcagc cacaaagaaa agtgcaatac
     3661 cgacgtactc aacccaaatg ttcggacgcc acagcagcca ggccaacgcc accatctggg
     3721 cagtggtttt cactttccca atccaggaga cggccacgct actgcgttta cccaactccg
     3781 ccatccattc gcgtagcgca gaaataataa tttcacgggc gatcatcgtt gccgccggta
     3841 atgtcaccca ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa
     3901 ctttatctgc cacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg
     3961 ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa
     4021 acggcgacca ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac
     4081 ggaacagtgt aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt
     4141 cagtaagatt acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt
     4201 ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg
     4261 taaaccttgc aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc
     4321 ttcaatggtt tccagggaac tggtattttt gaccttcgcc cgttttttac ggtgcccgcc
     4381 aatcgcgtga tcatgtgatt catcgcgaat atgctggata acatgcagcg cgggtgaatc
     4441 tggcggcaaa ctaaatccct caccttccgg ctcaaagaac agcgtttcca gtccagcctt
     4501 acgatctgct cctttggcaa cgccaagtag cagcggatga tttttatccc atgagacatc
     4561 cagttcggcg aagacatttt tcgcctgcgc aagctggcct ttgccgccgt cgataaggat
     4621 cacatccggg atcttactgt cgtcaatggc tttaccataa cgccgacgca gcacctgatt
     4681 catcgccgca taatcatcgc ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc
     4741 acgcagcggg ccgttagcat caaacaccac acaggaagcg acggtttgtt cgcccatggt
     4801 atggctgatg tcaaagcact ccatccgctt cacttccggc aatttcaaca cgctggcaag
     4861 cgcggtcagt cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc
     4921 cgcattggtg cgcgcgagtt tcagataacg cgccctatcg ccgcgaggtt tggtttgaac
     4981 attaatcttg cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag
     5041 attaaaatcg agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa
     5101 ctgacctacg aaggtttcta ccacctcgct cagttccgta ccgccaggca ctttcgggaa
     5161 atagctgcgg ctgccgagca ctttgccctg acgaatgaac aatacgtgga cacaagccat
     5221 gcccgcatcg aacgccacac caataacgtc aaggtcgtcg ccggtattgg aaacaaactg
     5281 tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga atacatgccg cttcttcaaa
     5341 ctccagattc tggctggcag tttccatacg gctaatgagt tgcgtaagca cctgatcatc
     5401 tttgccagac aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac
     5461 cagtccttca acgcacggtc ccagacagcg ccctatctgg tattgcagac acggacgcga
     5521 gcgattgcga taaacactat tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc
     5581 cagtgtttca cgtacggcat agccattcgg gaacgggccg aaatattcac ctttggcatg
     5641 ctttgcacca cgatgcatcg ccagacgcgg gtgggtatcg ccactcagga agataaaagg
     5701 ataagattta tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt
     5761 gtgttccagc aacagcgctt cggtttctgt atgcgtaacc gttacatcaa tttgctggat
     5821 ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg ctgcggaaat agctggaaag
     5881 ccgttttttc aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat
     5941 gcgataaacg cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc
     6001 actcactgac ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc
     6061 agctcaacat cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc
     6121 gttttcggac tgagattgag ctgttctgag atctcattga ccttctggcc cttagtgatc
     6181 atcagcataa tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct
     6241 ggttcgatct ggcttaacgc catttgttga gcgatatcag aagcaatgta acgctgccct
     6301 gaatagacag aacgaatcgc actcacgact tcctgcggag ccgcgccttt gctgaggtag
     6361 ccagcagcac ctgcctgcat gactttcgct ggtaaagggt tttctgtatg gaccgtaagc
     6421 atgatgattt taacatcagc tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca
     6481 atgcccggca tactcatgtc cattagcacc acgtcaacgg catttgtccg gcaccactta
     6541 acggcgtctt caccgcacga tgcctctccg acgactttta tacccttaat atcttccaga
     6601 atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa
     6661 ggaatatctc cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt
     6721 ttttattgca actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt
     6781 caattcgatt gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca
     6841 cagtaagccc tacgatgaaa tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca
     6901 gcccgctgaa tatgtaacat taattaaccc gaacaatacg ggcaaaaaac attc
//
LOCUS       DAZPFP010000188         6936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPFP010000188 DAZPFP010000000
VERSION     DAZPFP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.191"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            266..1798
                     /locus_tag="ACHUR4_003483"
     CDS             266..1798
                     /locus_tag="ACHUR4_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1456912.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(1850..2569)
                     /gene="fadR"
                     /locus_tag="ACHUR4_003484"
     CDS             complement(1850..2569)
                     /gene="fadR"
                     /locus_tag="ACHUR4_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1456913.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2790..4331
                     /gene="nhaB"
                     /locus_tag="ACHUR4_003485"
     CDS             2790..4331
                     /gene="nhaB"
                     /locus_tag="ACHUR4_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1456914.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4477..5007
                     /gene="dsbB"
                     /locus_tag="ACHUR4_003486"
     CDS             4477..5007
                     /gene="dsbB"
                     /locus_tag="ACHUR4_003486"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1456915.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5053..6321)
                     /gene="umuC"
                     /locus_tag="ACHUR4_003487"
     CDS             complement(5053..6321)
                     /gene="umuC"
                     /locus_tag="ACHUR4_003487"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1456916.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6321..6740)
                     /locus_tag="ACHUR4_003488"
     CDS             complement(6321..6740)
                     /locus_tag="ACHUR4_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1456917.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         1659 a         1759 c         1701 g         1817 t
ORIGIN      
        1 ttgaaaatat tattcaacat acggcttttt tatggtgagc tgactcacat ctccctgact
       61 ctgcggcaat atggcgtctt tagtatctcc tgctgaacgc cctttttatc cagcataaaa
      121 ttccgttcag aagcggatta gtggcactct gataataaga aatcgcaaag aaaaaaattc
      181 tccggcgacg cggtttttaa caggtgttct atgcttgaaa tgaggtgctc cacacagaga
      241 gcgccgacaa caatgaggtg cgcgtatggc gacgatcgat tctatgaata aggacaccac
      301 acgtttgagc gatggacccg actggacgtt cgacctgctg gatgtttatc tggcagagat
      361 agaccgggtg gcgaaactct accggctgga tacctacccg caccagattg aagtgataac
      421 ctcagaacag atgatggatg cctattccag cgtcggcatg ccaattaact atccgcactg
      481 gtcattcggt aaaaagttta tcgagactga acggctgtat aagcacggtc agcaagggct
      541 ggcctatgaa atcgtcatta actctaaccc gtgtatcgct tacttgatgg aagagaacac
      601 cattactatg caggcgctgg tgatggcaca cgcctgctat ggacataact ctttctttaa
      661 aaacaattgc ttattccgca gttggaccga cgccagttcg attgtcgatt atctgatttt
      721 tgcccgtaaa tatattaccg agtgcgaaga acgttatggc gttgatgaag tagaacggct
      781 tctggactcg tgccacgcgc tgatgaacta cggcgtggac cggtacaaac gcccgcaaaa
      841 aatctcgctg caagaagaga aagcccggca gaaaagtcgc gaagagtatc tacaaagtca
      901 ggtcaatatg ctctggcgta ccctgccgaa gcgcgaggaa gagaaaacgg ttgctgaagc
      961 gcgccgctat ccgtccgaac cacaagaaaa cctgctctat tttatggaga aaaatgcgcc
     1021 actgctggaa tcatggcagc gtgaaatcct gcgtattgtg cgtaaggtga gccagtattt
     1081 ttatccgcaa aaacagactc aggtgatgaa cgaaggctgt gcgaccttct ggcactatac
     1141 catccttaac catctgtatg atgaagggaa agtaacggaa cgttttatgc tggagttttt
     1201 gcacagccac accaatgtgg tcttccagcc cccctataac agcccgtggt acagcggcat
     1261 caacccgtat gccctcgggt tcgccatgtt ccaggatatt aaacggattt gtcagtcgcc
     1321 aacggaagaa gacaaatact ggttcccgga tatcgccggt tccgactggc tggaaacgct
     1381 gcatttcgcg atgcgtgatt tcaaagatga gagttttatc agccagtttc tgtcaccgaa
     1441 agtgatgcgt gatttccgct tcttcaccgt gctggatgat gatcggcata attatctgga
     1501 gatttccgct attcataatg aagaaggtta tcgggagatc cgtaaccggt tatcgtcgca
     1561 atataactta agtaatctgg agccgaatat tcagatctgg aacgtggatt tgcgcggcga
     1621 ccgttcgctg acgctgcgtt atattccaca taatcgcgca ccgctggatc gggggcgcaa
     1681 agaagtcctg aagcatgtgc atcgcctgtg gggatttgat gtgatgctcg aacagcaaaa
     1741 cgaagacggc agcatcgagt tgctggaacg ttgcccgcca agaatgggaa atctgtaaaa
     1801 acaacaaaaa acccctcgtt tgagggggtt gctctttaaa cggaagggat tatcgcccct
     1861 gaatggctaa atcacccggc agatttttct gcatccggtg ccaaatctcg ccactctcat
     1921 gcccatagcg acgcactgtt tcgtacacct gatcgtgcgc gccttcactg cacaacgccg
     1981 acagtttgtg gtagaagccc agcgccagac tgcgcgcttc cggattggcg aaatagtgac
     2041 gaccaatgcg cgtatacagc cctttcatcc cgttaaggat cagaccgtaa atcgggttgc
     2101 cggaagcaaa cgccaggccg cggaatatgt tgtaatccag ctcggcaaag gcatcggcgt
     2161 gatcggccac ttcattagcg gtagccagca cttcctgcgc tttatcggga tgctgacgaa
     2221 acgcggtgcg aataaaaata gtggaaatat tggtacgcac cgacagcaaa ttatcaataa
     2281 gctgcggcac actttcgtga tccagtcgcg ccagtgtttc aaggatattt aaaccggaag
     2341 tttcccagaa attattcacc ttcgtcggct tgccatgttg aatggtcaac cagccatctc
     2401 gtgccagacg ctgtaacact tcacgtaacg tagtacgcgt tacgccaatt aattctgaaa
     2461 gttcacgttc tgcgggcaaa atagtcccgg gagggaagcg gttattccag atactttcaa
     2521 taatgtactc ttccgcgaaa cccgccgggc tttgcgcctt aatgaccata gtgagatttc
     2581 cataacacag caaaacaaag ttggactcat cataccagag ggctaactac gctgatagca
     2641 gacaaaaaga agaaaaaggg atcaaggctt cattttccgc atctctttcc ccttgactat
     2701 caagacttgc ctcactcctg cctgaccgtt aagctttgcg tttaaacata aaaacatcat
     2761 tgattatttg taaggtaagg gaaaccatca tggagatctc ctggggccgc gcgctatggc
     2821 gcaatttttt gggccagtcc cccgactggt acaaactcgc cctcatcatt ttcttaatcg
     2881 taaacccgtt aattttcctc atcagccctt tcgtcgcggg ctggttgctg gtagcggaat
     2941 ttattttcac tctggcgatg gccctgaaat gctacccgct actccccggt ggtctgttgg
     3001 ctatcgaagc ggtattcatc ggcatgacca gcgcggaaca cgtccgtgaa gaggtggcgg
     3061 caaatcttga agtcttgctg ttattgatgt ttatggtggc gggtatctat tttatgaaac
     3121 agctgttgct gttcatattt acccgtttgc tgttaagcat tcgctccaaa atgctgctgt
     3181 cgctctcttt ttgcgtggcg gcagcgttcc tctccgcgtt cctcgatgcc ttaaccgtcg
     3241 tggcggtggt gatcagcgtt gcagtcggtt tttatggtat ttatcatcgc gtagcctctt
     3301 cccgtaccga agacaccgac ctgcaagacg atagtcatat cgacaagcat tacaacgtgg
     3361 ttctggaaca gttccgtggc tttctgcgta gcctgatgat gcatgccggt gtcggcaccg
     3421 cattaggcgg cgtaatgacc atggtgggcg aaccacagaa cctgatcatc gctaaagcgg
     3481 ctggctggca ttttggcgat ttcttcctgc gcatgtcgcc ggtgaccgtt ccggttctga
     3541 tttgtggcct gttaacctgc ctgctggtag agaagctgcg ttggtttggc tacggtgaaa
     3601 cgctgccgga gaaagtccgc gaagtgttgc aacagtttga cgatcaaagc cgccaccagc
     3661 gtacccgtca ggataaaatc cgtctgattg tccaggcgat tattggcgtc tggctggtga
     3721 ctgcgctggc gttgcatctg gcggaagttg gcttgattgg tttgtcagtc attattctgg
     3781 caacatcatt gaccggtgtc accgatgaac atgctatcgg taaagccttc accgaatctc
     3841 tgccattcac cgcactgttg acggtgtttt tctcggtagt cgcggtgatt atcgaccaac
     3901 aactgttttc gccgattatt cagtttgtgt tgcaggcatc tgaacatgct cagctgtcgc
     3961 tgttctatat tttcaacggt ctgctgtcat ccatttcgga taacgtcttc gtggggacga
     4021 tttatatcaa cgaagcgaaa gcggcaatgg aaagtggcgc tatcacgttg aagcaatacg
     4081 agctgctggc ggtcgccatt aataccggta ccaatctgcc ctctgtcgct acgccgaacg
     4141 gtcaggctgc gttcctgttc ctgctgacct ctgcactcgc gccattgatt cgcctctctt
     4201 atggccgcat ggtgtggatg gccctgcctt acaccctcgt actgacactc gtcggcttgc
     4261 tctgcgtcga gtttacgctt gcccctgtaa ccgaatggtt tatgcaaatg ggctggatag
     4321 caacgctttg ataacaactt accgggcata taaatgcccg gtttgccttt tcgcccgata
     4381 attgtccaat tgcgtcctat ttttgctgcc tcctggtggc gcagcgaatg aattggttta
     4441 aactgcgcac tctatgcata ttgcagggaa atgattatgt tgcgattttt gaaccaatgt
     4501 tcacaaggcc ggggcgcgtg gctgttgatg gcgtttactg ctctggcact ggaactgacg
     4561 gcgctgtggt tccagcatgt gatgttactg aaaccttgcg tgctctgtat ttatgaacgc
     4621 tgcgcgttat tcggcgttct gggtgctgcg ctgattggcg cgatcgcccc gaaaactccg
     4681 ctgcgttatg tagcgatggt tatctggttg tatagtgcgt tccgcggtgt gcagttaact
     4741 tacgagcaca ccatgcttca gctctatcct tcgccatttg ccacctgtga ttttatggtt
     4801 cgtttcccgg aatggctgcc gctggataaa tgggtgccgc aagtgtttgt cgcctctggc
     4861 gattgcgccg agcgtcagtg ggatttttta ggtatggaaa tgccgcagtg gctgctcggt
     4921 atttttatcg cttacctgat tgtcgcggtg ctggtggtga tttcccagcc gtttaaagcg
     4981 aaaaaacgtg atctgttcgg tcgctaatcc attcggcgct cctgcgggag cgcttttttc
     5041 ctgccgctat atttatttga cccgcagtaa atcagaactt cgcgttgtat aacgtggtga
     5101 aagcatagct cgcttcatct gccattgttg ttggatcccc tgcccggcaa aatagagtgt
     5161 tcctctgccc tctttagcat tcagagtatc cattaccgcc atcaattgct cactcccggg
     5221 gcgcggtgcg ttgtcatcga ataaattgag ctgcgcgact ccctgactga agaaatcccc
     5281 cagcatcacg cccgcttttt ggtagcgatg gcccgcttgc cagatggcat ccagagatcg
     5341 cgtagcagcg ttaatgatat ccctgctgtc ctgagtgggc gtcagcagtt ttaccgacgc
     5401 gctattgccg taataaggtt cattgagcgc aaatggtgac gtcttaataa atgtggagat
     5461 aaaccgacaa tattgatgct cactgcgaag tttttccgcc gcccgggcag cgtaactaca
     5521 aatggcctgc cgcatcgacg tataatccgt gatgcgttca ccaaacgagc gggaacagat
     5581 aatttcctgc ttcgtcggtg caaactcttc cagttgcaaa cagggttcgc cgcgcagttc
     5641 acgcaccgtt ctttcgagca tgacattaaa atgtttacgg ataaaccgga tatctgtatc
     5701 cgccaaatcg agaacggttt tgatccccat cgcgtccagt tttttgctga tccgccgtcc
     5761 aatcccccag acgtcatcca cggggagagc agacattaat ttacgctggc gttccagatt
     5821 tgataaatcc accaccccac ccgtctgccg ctgccatttt tttgccgcat gattggcaag
     5881 cttagccaac gttttggtct gggcgatccc cacaccaaca gtaagatggg tacgttgtag
     5941 caccgttgcg cgaatttctc tgccaaaatc agtcagatcg cgacaattac gcacacctgt
     6001 cagatcgcag aatgcttcat caatactgta aatctcgacg cggggcgata gttcttccag
     6061 cgtcgacatc acccgattgc tcatgtctgc gtaaagctca taattgctgc taaagcaaac
     6121 cacaccacag cgacgaaaca gatctttttg tttgaaccag ggatcgccca ttttaacgcc
     6181 cagcgccttt gcctcagcgt ttcgggcgat aacgcaaccg tcattattcg atagcacaac
     6241 caccggttta ccccataaat cagggcgaaa caccgtctca cagctggcat aaaacgcgtt
     6301 tacatcacag agggcaaaca tcagcgcatc gccttaacga cgtggatcac cacaccaaag
     6361 acatccagcg tatcttcgct actgatggta atgggcgagt acgcactgtt catgggaata
     6421 agctgtaccg tcgggcgtag ttgcaatttt ttcaccgtaa actcgccgtc aacagcagcg
     6481 atgacaatat caccatggct ggcggtaata gcactatcga caatcagtaa atcaccgtca
     6541 ctaattccac catcaatcat agaatcacca cttgctttga cgaagtaagt cgcgctggga
     6601 tgctggatca acagttgatt cagatcgatg cgctgttcaa cgtaatctgc tgccggtgaa
     6661 ggaaagccac actgaacaag atcgctaaat agcggaaaag tcacaatttc gcggagatcc
     6721 gcaggcttga taaacaacat aataatctgc ctgaagttat actgttttta tatacagtgg
     6781 tctgttcttg ccagcagatc aatactgatt caggctatca atatttgtcg ctgcatagac
     6841 tgctgatttt tcgttctctt atcctgtgct cacgtggcct tctggcgacg acgctcatcc
     6901 agcagaaatg aaaaatatca cccggcaaaa aaatag
//
LOCUS       DAZPFP010000189         6921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPFP010000189 DAZPFP010000000
VERSION     DAZPFP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.192"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHUR4_003489"
     CDS             <1..694
                     /locus_tag="ACHUR4_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1456918.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHUR4_003490"
     CDS             complement(1033..1803)
                     /locus_tag="ACHUR4_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456919.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHUR4_003491"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHUR4_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHUR4_003492"
     CDS             complement(2294..2515)
                     /locus_tag="ACHUR4_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456920.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHUR4_003493"
     CDS             complement(2516..3199)
                     /locus_tag="ACHUR4_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1456921.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHUR4_003494"
     CDS             complement(3263..3574)
                     /locus_tag="ACHUR4_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456922.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHUR4_003495"
     CDS             complement(3571..4473)
                     /locus_tag="ACHUR4_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1456923.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5871
                     /locus_tag="ACHUR4_003496"
                     /pseudo
     CDS             4913..5871
                     /locus_tag="ACHUR4_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5871..6266
                     /locus_tag="ACHUR4_003497"
     CDS             5871..6266
                     /locus_tag="ACHUR4_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1456924.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6449..6829
                     /locus_tag="ACHUR4_003498"
     CDS             6449..6829
                     /locus_tag="ACHUR4_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR1456925.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1891 a         1790 c         1676 g         1564 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt tgcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttgc cccccgactg ttaacgctaa
     6061 tcagcgtttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gcaatctttc tatgttaaaa aaataataaa agaagagcag gttcagtcaa ttacctgctc
     6301 ttcttttata catactaaaa aatcagattt ttacgaatat tcagattatc ataaaaaatg
     6361 caacagacag gaatgcaata attttgcatt atctcgttgc ttgtaatgca aatatgcatt
     6421 attgtcagtg tgtgacaata acagagagat gacgatggcg accattaacg cacgtattga
     6481 tgatgatata aagaaccaag cagatgaagt gctgaagctg atgaatatca gtcaaactca
     6541 ggctattgca gctttttatc agtatattac tgaacaaaag aaactgccat ttgtgatcac
     6601 ctcaattgta aaaacgccgc atgatttatt acgtgaaagt acagacatgt tagctgaagc
     6661 tcttgcagtg atttccaatc tacaggtgtg gactgaacag caggatggga ttggaaaagc
     6721 aaagctaatg gagtattacc gccgcctcga cgcactgtac tgttgtgcaa aagagaaaat
     6781 aggtcttctt tcagataacc gggatgctga actaggctgt gtcccttaat catctgagtt
     6841 atagtaactg tctgtttaca caacatattg aactatggct cgctacgacc ttcccgatga
     6901 ggcatggacc atcatcaagc c
//
LOCUS       DAZPFP010000190         6589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPFP010000190 DAZPFP010000000
VERSION     DAZPFP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.193"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1077
                     /locus_tag="ACHUR4_003499"
     CDS             <1..1077
                     /locus_tag="ACHUR4_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR1456926.1"
                     /translation="AAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLHDAYKASDERLYVNKQNKNSRS"
     gene            1120..1623
                     /gene="yeaK"
                     /locus_tag="ACHUR4_003500"
     CDS             1120..1623
                     /gene="yeaK"
                     /locus_tag="ACHUR4_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1456927.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACHUR4_003501"
     CDS             complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACHUR4_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1456928.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1898..2344
                     /locus_tag="ACHUR4_003502"
     CDS             1898..2344
                     /locus_tag="ACHUR4_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1456929.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACHUR4_003503"
                     /pseudo
     CDS             complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACHUR4_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3216..4396
                     /gene="nimT"
                     /locus_tag="ACHUR4_003504"
                     /pseudo
     CDS             3216..4396
                     /gene="nimT"
                     /locus_tag="ACHUR4_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="ACHUR4_003505"
     CDS             4451..4798
                     /locus_tag="ACHUR4_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1456930.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACHUR4_003506"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACHUR4_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1456931.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="ACHUR4_003507"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="ACHUR4_003507"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1456932.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /locus_tag="ACHUR4_003508"
     CDS             6315..6413
                     /locus_tag="ACHUR4_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1456933.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACHUR4_003509"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACHUR4_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1456934.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1594 a         1596 c         1616 g         1783 t
ORIGIN      
        1 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
       61 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
      121 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
      181 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
      241 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
      301 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
      361 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
      421 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
      481 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc
      541 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc
      601 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
      661 tacgtccatg acttacctgg cggcattcgt gtctcgttat ccattgatat tctttacttt
      721 atcacgtctt cgaggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
      781 atggtccgga tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
      841 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
      901 ctggtgcaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa
      961 ataaatgaca ccctcggtca tcaggagggg gatttagcga ttacgttaca tgatgcgtat
     1021 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     1081 ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga
     1141 agcgtgacgc atcagcagtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     1201 gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg taccgctcta
     1261 ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt
     1321 ctggcgatcc tcgcagccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     1381 ggattacgcg cttcgttggc cagcccggcg gaagttgatg aactcacagg ctgtgtcttc
     1441 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc
     1501 ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg
     1561 aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     1621 tagctagcct atccgttcaa taagcagaac ggacaaaacc agcacaaccg caatagcaaa
     1681 aaacgatgat gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg
     1741 ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg
     1801 cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcataaa
     1861 gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     1921 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     1981 tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     2041 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     2101 cgggacgcta ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc
     2161 aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacgt taatgggcag
     2221 ccagccgcaa ctggttgctg ggctgctggt gggcactgtt ttaggcgtag cgctgtttcg
     2281 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     2341 gtagttaatc ccgcaatata tcggcctggc gtttgcccca agcctttctt aaacatagta
     2401 ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct
     2461 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     2521 aaccccgtct ctttcactat caggcgggca agattgcgtt cactcatcgc aaaaaagcca
     2581 gcccattgcc ccaatgcccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg
     2641 cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt
     2701 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     2761 ctgtttgcca gggttaaaat caattcccgg cataagggcg atattttcaa tgtgcaacat
     2821 ggtcggcatt gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt
     2881 cacttgattg ctatgcttca ccccgcccgg tatccaaacc gcatattgtg gcggcaccat
     2941 ccacaaagca ttttccaccg tacaggtaat tgcaccatgt aatgccagta tcagctgtcc
     3001 tttgcggtgc tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc
     3061 gaccgcggct tcatggtgtc ggtgggttca tagccattga gattcaacct gtgcatcatt
     3121 ttgtccgaac ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg
     3181 taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg
     3241 gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca ttacgcgtca
     3301 cctttaccgg cgcagcgccg ttactggata cgattcgttc cgcttactcg ctgacgacag
     3361 cgcaaaccgg cttattaacc accctgccat tattggcctt tgcgctaatc tcacctctgg
     3421 ctgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga
     3481 tctgtgcagg tatcgcaatt cgttctctcc cttcgcctta cttattattt ggcggtacag
     3541 cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta attaaacgcg
     3601 atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag
     3661 cggcactggg atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg
     3721 cgttgttcat gctgatgtgt tttcctctgc tggctctttt tttatggctg ccacagtggc
     3781 gaagtcagca acatgcaaat ttgagtacct cacgcgcctt acatactcgg ggtatctggc
     3841 gttcgccact tgcctggcag gtaacattgt ttcttgggat caactcactg gtctattacg
     3901 tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg
     3961 gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg atcgcacttt
     4021 tcttacatca tgtgaaagac cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag
     4081 tgggcgcggt tgggctctgc tttatgccag cgcacgcgat cacctggact ctgcttttcg
     4141 gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt cggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtcttgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggct gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcg
//
LOCUS       DAZPFP010000191         6533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPFP010000191 DAZPFP010000000
VERSION     DAZPFP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.194"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUR4_003510"
     CDS             complement(<1..353)
                     /locus_tag="ACHUR4_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1456935.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..934)
                     /locus_tag="ACHUR4_003511"
     CDS             complement(350..934)
                     /locus_tag="ACHUR4_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1456936.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(921..1298)
                     /locus_tag="ACHUR4_003512"
     CDS             complement(921..1298)
                     /locus_tag="ACHUR4_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1456937.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHUR4_003513"
     CDS             complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHUR4_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1456938.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1748..2776)
                     /locus_tag="ACHUR4_003514"
     CDS             complement(1748..2776)
                     /locus_tag="ACHUR4_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1456939.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2834..3181)
                     /locus_tag="ACHUR4_003515"
     CDS             complement(2834..3181)
                     /locus_tag="ACHUR4_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1456940.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(3218..4723)
                     /locus_tag="ACHUR4_003516"
     CDS             complement(3218..4723)
                     /locus_tag="ACHUR4_003516"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1456941.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(4713..6306)
                     /locus_tag="ACHUR4_003517"
                     /pseudo
     CDS             complement(4713..6306)
                     /locus_tag="ACHUR4_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6303..6506)
                     /locus_tag="ACHUR4_003518"
     CDS             complement(6303..6506)
                     /locus_tag="ACHUR4_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR1456942.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
BASE COUNT         1421 a         2069 c         1574 g         1469 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacatc gggattttca
      481 ccacatcaat ggggtaacga tttttgccgt caatacgccg catcacatgc cagcgaccat
      541 tcgccagttg ctgaataaac gcatcccgga aaaggtattt ccccactttc agtacgctgc
      601 cacgatgacg tgagttgctg ccatgccggg agcgggccag tctgacctgt gcggtaccca
      661 gcttaatggc gggcaggtta ccccggttaa cacgcatgcg ggcatacatt tttcctgagg
      721 ggctggcttt aagcagcctg acacgctccc tgaccgtttt caggggaata cctttcacct
      781 ggttatcccc ggcaacggta ttctgcgcaa cctgccgtgt ggcgacagaa acaatctttg
      841 ccgccacacg gtttaccgcc catgcgctgg tctgtggcac catgcgggta tcgaggctgt
      901 tcagattgcg gatggcattc tcaagtccct tcatcatatc gcctcattct ggcattattc
      961 tgtggggcag gactgccccg ccttaaccgg atataacagc atcccccgtc atccggagta
     1021 atacgatcca caaagaaaga atcatccccg atagttaacg tatcctgacg gcgtaactgc
     1081 gatatatctg ccgttttcac aaacaggcac ggtgaggaat cctcaagccg gatccccccg
     1141 gcaacaaaag aaatactttc gggatcatca aaaacccctg tcaggtgtgt cccctcaagt
     1201 tctccggacg taatgacggc gctgatcccc atatcacgga gaatgacttc attgacaccg
     1261 gcaagcgcag catcaaacag attatcgaaa ccgtccacga catctcctgt tactgccttt
     1321 ttgccagacc acctgccgcc atgctggcag ccacctcagc agaaacacga aacgacgttc
     1381 ccggtgaaac aaatatcact gaaccattac attttgtatg cagtgtcgcc agtgccacca
     1441 ccgtaacctg ccccgtttcg tctccggtaa caatgcctgg ctgttcagga actgactcgc
     1501 ctttatgcgc ggtcggatca tctttcgatg gtgagtacgt atccggctct gcgacctccc
     1561 ccgcatctcc aagctcttcc tccagttccg ccactcgctg cgcaatatct gccgcactac
     1621 cggagacatc cggttcacgc cccagcctct cagccagctc acaaagacgt ttcaggtttt
     1681 cttctttcgt agccataccc gctcctgatt gaatgaaaaa cgggagcatt tagctcccgc
     1741 tcacggatta tttcacctgc accaccacaa actcatccgg atccggcagc accatcagcg
     1801 gcgcggactg cgtcatggtg aattcacgcg ccggatcacc cacggtcagc cagtgtttcg
     1861 gataacggga agacgccacc acgccttcag acaatgcctg cgcatcctga atggcaccgt
     1921 agcaacgaat accctctgcg gccgtatttc ccagtaccag tgtgccgtcc ggcagataac
     1981 gtttttcggt accgtcctct gccacatagg acgtttttgc caccacgatt gccagatcac
     2041 cataataccc tttgaaggac accaccgccc ccaggtcttt cactgccgtt tccagctgtg
     2101 aatttgagcc acgacgggta tccagttttt cgcggaacag cttaaagcca ttcagcagac
     2161 gccagacctt accatccata atggcaatat tgatggtgcc ggaagcaaaa tcacagtacg
     2221 catccagatc atgcgtcgga tcaaaggtat cgcggtcctg agcgctccac tccgtccctc
     2281 ctgcctgcgt aatgttattt cccaccgacc ggccaaaatc gacctcaaca gtttcaaact
     2341 gctctccttc catggtgtat ttgccataca gcacggcatt caccgcctgc atctcttcca
     2401 cctggacaat ggcgtgctct tcctgtttga ggttatcggt gatgatacgc aggcgacggt
     2461 aagccgggcc gttcagctgg gccggatctt caccgggaag acgctccacc gcctgctggt
     2521 aattaaattc gtgtttcggc ttaacgtagc ccgggcgcag tacacgagtt tcaccgccac
     2581 ggtgacgcaa cacctttcct tcaacaaccg gggagacata ggccgccacc ggcgtttttc
     2641 cggtaatttt gtccagcatt acctcttcgg tatggaaatt caccgtacgg cggaaaaaca
     2701 gctccagaaa cagcgcacga aatttaactt tttgttcggt ataaccgagt aactggcggg
     2761 tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc taccgcagtg
     2821 acctgataaa atgttacggc agcgctgcgt gactcagggc actgccggca aaggcattcg
     2881 cctttttgtg tgtatccact ctctcaggcc agtggatggc ctccgtcgca aaggtccccg
     2941 acttgtaata cgtcagcacc gtctctgtac cttcaagcgg cagtaccagt atgccaacca
     3001 catggcctgc cttctgtccg tcccagacca ccagtttccc ggtatcctca tccagcatca
     3061 ggggcgtcag agccggtgtt gccgaggaaa tcccgctgca gcctgtggcg gtatgggccg
     3121 gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatggtt ttcgttacca
     3181 tattgtaaaa acctcatatt gatggtcagc actgacttca tggcatggcc atgagcattt
     3241 tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc gctgccggtg
     3301 aatggttcgc catgaaatgt tcaaacatgg cggttgtgga tgcagaaacc ggttctgccc
     3361 tgcctgaggc tgccgccagt acagcccggg cattctccac ggtcatcccc ggacaggccg
     3421 ccagtttttc agcctgcgcc tctgcccctt ttgcctcatc cagggccata atctgatcac
     3481 gaagtgaggg tccggcattc gcctgcggtg aagcagccag aatcggacgg gctttttcca
     3541 ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgtcct ttcgcctctt
     3601 cacatgccat aatgcgatcg gcttcactct gcgcagatac accagattgt tgcggtgccg
     3661 ccacggccag aatcgcccgg gcctgctcaa cgctcatacc ctgttgccct gccagcatcg
     3721 tggccagttg ttcacgtcct ttcgcttcct gacacgtcag gatccccatc actcgctggt
     3781 tctcctgcgc ggcagcatcc gttgcagtta attgcggcat agtgcctcct gtatcgtgtg
     3841 tgttcagcgt cgcagccatc acgctgatgg cgtccgacgc attgattaat tcatccgcca
     3901 gttcggcctc aataccggac tgaccttcaa aaacggcggc ctctgttccc ataaccgcat
     3961 ccacagataa accggtatac atcgccactt tttcagcaaa catccggcgc gccgcatcaa
     4021 tgcgctgctg catgtcctgg cgaacctttg ctggcaggga ttcaaactga ttgccatcca
     4081 ccttgtgcga tccggcgtaa atcagcgtga tatccacacc ggcctgtgcc agatgaccgg
     4141 catagctgac atggcccatc atcaccccaa tggagccgat acgggatgtc tgggtaacca
     4201 gccgtcggga gcaggccgat gccagtaaca tggcggctga acaggccgtg tcattgcaca
     4261 gtgcccagac aggcttcagc tgccggagac ggtaaatcat gtcagcgcag tcaaacgcgc
     4321 cggtggcctg cccgccagaa ctgtcaatgt ccagcagtac gccccgtacc tgagtgtccg
     4381 ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc agtcattccg gaaaatggcc
     4441 gcatgccacc cagccggtgt accagcgtgc cggtcaccgg cagtacagca ataccgttca
     4501 ccacccggta aacacgggca ggtcgtttgc ctccggccat gtactcgtcc gtttcagcca
     4561 gcatcccggg agcgtcaagc tgtacctgct gttgcggtac cgaaaggctt gctgcgccca
     4621 tctcacgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggct
     4681 cattgaatgc cgcagcgata atatgtgaaa gattacgtct cacgtgatgt tgtctcctct
     4741 tccggcctgc gactctccgc tatctgctgc tgatacgcct gggctatcca caccggacgt
     4801 gagagtccgg ctttttcgcg ctctgcggat tccctgacct gctggcggaa aatgtcctga
     4861 taatcttcgc ccatcagtgc cagttctttc tcatacgtgc tcagtccggc ctcaatgcgc
     4921 atcaccgatt cctggacctc cttgagcccg tcaatggcca ttcttccgga gctaatccac
     4981 tctgcccgtg accaggctga acgcgcctgg taaaaatcaa aacgcgcccg tggcggacga
     5041 ataatccccc gaagaagcgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg
     5101 gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc ggaggcgcgg
     5161 gcacttgagt aactgacctt cgagtaatca cgggacaact gttcgtagga aacgccaaga
     5221 ccggctgcaa tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc
     5281 tgcgctgtct gcaggttcag cgcatcgcca ggaaaaaagg tgcggaattt taacaccgcc
     5341 cagcgtcacg ttattcgtgt cataccagcg ggtgaacttc tccagaatat taataagcgg
     5401 attatcctgc tgaccttgcg gtgcccccgc gatatattca aatgcctttt cggtatcaag
     5461 ttcactttcg attgtggctg catacatcgc cttcacaatg gccgactgaa gctgcgtggc
     5521 ctgcagggag tccagcatct tcatccgctc cattacgctg taaaactgat tagccccacg
     5581 ggtctgcccg tcctccaccg gctcaaaaat atgcagcatg gccggacgcc cggtgggaag
     5641 ctcacgcggg atccgctccc agcgtccggc accagagaac ggaaaatcat cctcacagat
     5701 atggtacgca atcgcccgcc cataccgatc gacctcaacc ccggcccgca gaaagtggtt
     5761 ccccataccg tgtcctggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     5821 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaaggc
     5881 atgcacgccc accccttcac ggataaattc cgtaaacgtg cgtttccctt ccacgtcgat
     5941 ctcgccaaac attccttctg cgtattccga ccaggctgct tccacctccc cgacaaaact
     6001 ttttgccgtg gtctcccgca tccccagcca acgccagttc agacggtagc tgataagaaa
     6061 catgtgcccg acgatatgat ccttatgcag ggccaccgca ttagccgcga taccgttatt
     6121 gcgcaccaga tcatctgccc gggcattccc cagacgcaat gcaggcaaca gggccgcatc
     6181 ggcactctgc gccggtggta accactctgc catttgcccg ccaaatcctg cgccgcctcc
     6241 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     6301 tttcataacc tcactcccag cggtcggcgg cgtcgggtcg tacccagaac cgactccgca
     6361 tcgttgatcg cacggttaag ctcattcagg gacgctgccg tatattcgat cctgcgaccg
     6421 tctttctgta ccgacacaac ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc
     6481 gcctgcagtt cagcgactgt aaccattcac tcctccggac agcttcgctg cca
//
LOCUS       DAZPFP010000192         6520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPFP010000192 DAZPFP010000000
VERSION     DAZPFP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.195"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUR4_003519"
     CDS             <1..115
                     /locus_tag="ACHUR4_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456943.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHUR4_003520"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHUR4_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1456944.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUR4_003521"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUR4_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="ACHUR4_003522"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="ACHUR4_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1456945.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAZPFP010000193         6467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPFP010000193 DAZPFP010000000
VERSION     DAZPFP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.196"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(135..989)
                     /locus_tag="ACHUR4_003523"
                     /pseudo
     CDS             complement(135..989)
                     /locus_tag="ACHUR4_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1097..1375
                     /locus_tag="ACHUR4_003524"
     CDS             1097..1375
                     /locus_tag="ACHUR4_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456946.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1376..1903)
                     /gene="gadE"
                     /locus_tag="ACHUR4_003525"
     CDS             complement(1376..1903)
                     /gene="gadE"
                     /locus_tag="ACHUR4_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1456947.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2702..3274)
                     /gene="hdeD"
                     /locus_tag="ACHUR4_003526"
     CDS             complement(2702..3274)
                     /gene="hdeD"
                     /locus_tag="ACHUR4_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1456948.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3529..3861
                     /gene="hdeA"
                     /locus_tag="ACHUR4_003527"
     CDS             3529..3861
                     /gene="hdeA"
                     /locus_tag="ACHUR4_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1456949.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3977..4303
                     /gene="hdeB"
                     /locus_tag="ACHUR4_003528"
     CDS             3977..4303
                     /gene="hdeB"
                     /locus_tag="ACHUR4_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1456950.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4367..5013
                     /locus_tag="ACHUR4_003529"
                     /pseudo
     CDS             4367..5013
                     /locus_tag="ACHUR4_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5055..5585)
                     /locus_tag="ACHUR4_003530"
     CDS             complement(5055..5585)
                     /locus_tag="ACHUR4_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1456951.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5741..6307)
                     /gene="slp"
                     /locus_tag="ACHUR4_003531"
     CDS             complement(5741..6307)
                     /gene="slp"
                     /locus_tag="ACHUR4_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1456952.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1872 a         1350 c         1391 g         1854 t
ORIGIN      
        1 tcgatatcga agcattgagc atttttaact cgttttatct tgaatgcaat cggcgcataa
       61 cttattgaat cccatcaaat cccgtctttt tttacccatt cccggttatc ttgaataaat
      121 tacctagtca taaatacatt ctggcggcta ccttcatcca ctaatgccgt gacatacatg
      181 ccaggcagca agtcaccatt tgggttgggg aaaatcgccc gtaacgtcac ggagcccgtg
      241 gtttcatcaa ccgtcgggtc ggagaatttc agcgtgccgg tctggctgta acgtttacca
      301 ttttccagat tgagctgtac tggcgtactg ccctgaacct gtttgatttg cccactggcg
      361 acctcttctt tcatgcgtaa gaaatcttgc accgactgcg tgagatcgac ataaatcggg
      421 tccagacgtt gtacggtaac cagcgaatct gcctgattag cggtaacgag tgcgccgacg
      481 gtcaccgacg atttcccgct gacgcccgta atcggcgagg tgacattcgc gtattgcaga
      541 ttgatcgtcg cctgttcaac agccgctttg gcgacggtga cattggcttc tgcttcattc
      601 agctgggtgc gcgcggtgtc gtaatcctga cggctaacgt ggttggtctt cagcaacgat
      661 gcctggcggt taaaggtgat gcgggcattg ctggcggtag agagcgcttt cgccagcgag
      721 cctttggcgg agtttagctc ggcctgtaaa ggtgcaggat caatctgata cagcgaatcg
      781 ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa tgataatacc gcccacctgg
      841 ggacgtatct cggcaacttc ataaggaacg gttctaccgg gcaattcgct caacacattg
      901 accgaaccag gggagagtgt gacgacaccg acctcaggcg tcatggcggc ggcgttttcc
      961 gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga ataacaacgg tattaacagc
     1021 tttcttcttc tgttcatttt agtccctgaa aattcttgag aaataacggt tctttaaatt
     1081 gcgcgcgagg agagatatga gggatttaca gaccagcggc atagtggggt tgtcagccag
     1141 taaggtggga tacaggcaca gtgatcgaca tggtgaggtc aacgacaaaa aaaatgttgc
     1201 cgttctgcca acagttcctg ccagcattcg ggcaacgaag ggttctactg gtggatacac
     1261 ataccagggg aatcaagtcg ttttagcctc tcctttagta atcaccgggg gtaatgaaat
     1321 cagcatctgc atccctcgtc atgccagcca tcaatttcag ttgcttatgt cctgactaaa
     1381 aataagatgt gatacccagg gtgacgatgt cgctcatacg tcttagtttc agcttgtaca
     1441 taatgttgaa tttctgaatc tttaatgttt gttgcgaaat attgagcgtc gacgtgatat
     1501 tgctttcatt tttatttttc agaatcagtt gcataatttt gaactgatta tgtgaaaaac
     1561 ataattgtga attcttatgg ggcaagtgtt taccataagt aatctcaaga agatttttga
     1621 tactttcttt gcggctaaca aaataaatag gcgctttagc ttttagttta ctgatgtgat
     1681 aaggtgccaa aacaataatt ctctcggcat ctaatttctc cagaaattta atcgcttctt
     1741 catcaaggat atgattgtga taggtatcaa tgataacctt gaagggttta ttgcctactt
     1801 ttttgatctc tgacagggaa ttgattttta tcatttcgtg attatctttc aactgccaaa
     1861 agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat cataacttgc tccttagccg
     1921 ttatcgtttc gaacatgtca tccttttcat tatttacatc cttgtccgaa tcgttgttca
     1981 atatagtaaa cgcctataag aactttgccc ttcgtgaact aatacgtcta ttaacactaa
     2041 tcaatacttc agatttatcc atcacgcact ccatatattg atcctaagta agattcctgg
     2101 ttgttatcag cttgtaaata gtaaacaaaa agttaacgtt tgcggattac tgcccaagaa
     2161 taagttatca ataaccagtt tgtgatctct gaagaatatt actaaagtta aaatgtaatc
     2221 cgatttaaat atcgagtctc cttgtttcga cttaagctgg caattggatt gccagctttc
     2281 ttttaacgca ttgaaattac ctgagtaaat aacgcattca tgtcttcagg tttttatata
     2341 gcgtctggag acacggatat ttatgcaatg taatcaacgc cagttgcaaa aaaatgaacc
     2401 cggattactt atttaaccgc ccatagcatc aacgcttacg gaactctttg ttcactttcc
     2461 cacagcactt ttaaaacatt aaacctgact acggaaaata tcagccatga taatgtttga
     2521 aatggctaat ttgccatgga gtgaaaaaaa ttagatgaaa ttcagtaggt tgaaataatc
     2581 actagcaggt aattatttca atgatagtgc gcaattgatc tacaacactg cgtagcggag
     2641 agagtattaa tcggatcata gtcacatcaa gtgactatga tccgggtgac aaccggggta
     2701 attattgctg cttaacgaac aaactggcga agctgaacag gctggcggcg ctaaatatca
     2761 gttcaattcc caccagtgtg gaaaccagcg ttacagacac catcggcgtt gcaccaagga
     2821 atatccaggc aatgacgata tccagcacac caataacgag ctgtagccag ctgcctttca
     2881 ttgaacgctg acgataccaa ctcatcaggc gaataacccc tgcaacacag aacaaaccgg
     2941 caataaatgc cgcaatggca aaaatgccca gctccggtgc acggatgaag aaatagccga
     3001 tcaataaata ggcgactgcg acgaggaaac cggataatac cggccagaaa ttatgactgc
     3061 ggttgctgaa taacccgaca ataagcgcaa tacccgagca gattaataat gcacccacta
     3121 ctgtgcttaa aatatcgcca gagacgaacg ggaaactgat acacagcaac ccgacgataa
     3181 acagcagcac ggcaataaac tggattgctc tgcgatgctt tttcagcatc tccagatcaa
     3241 acttcaaaat tgttgcctta tctatatata acatagaacc accctataaa attaagaaga
     3301 aaatcccctg ctatcaatct atgccaaaaa cgcgtctaag aatgcagtcg atttaataaa
     3361 aatttcctaa ttgcagtatc tgatgcatct gtaactcatt gtattgaaat aaaaatatct
     3421 gattttgata ttttccatca acatgacata tacagaaaac caggttataa cctcagtgtc
     3481 gaaattgatt cgtgacggct ctttcacttt atagttgagg atattacgat gaaaaaagta
     3541 ttaggcgtta ttcttggtgg tctgcttctt ctgccagttg tgagcaatgc agcggatgcg
     3601 caaaaagcag ctgataacaa aaaaccggtc aactcatgga cctgtgaaga tttcctggct
     3661 gtggacgaat ccttccagcc aactgcagtt ggttttgctg aagcgctgaa caacaaagat
     3721 aaaccagaag atgcggtttt agatgttcag ggtattgcaa ccgtaacccc agctatcgtt
     3781 caggcttgta ctcaggataa acaagccaac tttaaagata aagttaaagg cgaatgggac
     3841 aaaattaaga aagatatgta attccgggaa tgcgttacat cgtacttcct tgcatattga
     3901 acaggccgga atatcttctt taaaagcagc tattcctcct gttcatatat aatctctata
     3961 ttgaatgggt tacaaaatga atatttcatc tctccgtaaa gcgtttattt ttatgggcgc
     4021 tgtagcggct ttgtcactgg tgaacgcaca atctgcgttg gcagccaatg aatctgctaa
     4081 agatatgacc tgccaggaat ttattgatct gaatccaaaa gcaatgaccc cggttgcatg
     4141 gtggatgctg catgaagaaa cagtatataa aggtggcgat accgttactt taaatgaaac
     4201 cgatctcact caaattccta aagtgatcga atactgtaag aaaaacccgc agaaaaattt
     4261 gtataccttc aaaaatcaag catctaatga cttgccgaat taatgaggta caagtaaaaa
     4321 ggagtagcaa gttgagccat cttgctgctc ctttttgcat ttttatatga cagcagaatt
     4381 tattatacgt cttatactcg cggcaattgc ctgtggcgct attggcatgg aaaggcaaat
     4441 gcgcggcaaa ggagcagggt tacgcacaca tgtattaatt ggcatgggaa gcgccctgtt
     4501 tatgattgtt tcgaaatatg gttttgctga cgtgctgtct ttagatcacg tcggactcga
     4561 ccccagccgt attgctgctc aggtggtgac gggcgtcggg tttatcggtg caggtaacat
     4621 tctcgttcgt aaccaaaata ttgtaggtct gacgacggca gcggatatct gggtgaccgc
     4681 cgcataggta tggttattgg cagcggtatg tacgaactgg gtatttatgg ttcagtgatg
     4741 accttgttgg tgctggaagt cttccatcaa ttaaccttcc gcctgatgaa caaaaattac
     4801 catttacagc tgacattagt gaacggtaat acggtatcaa tgctcgactg gtttaagcag
     4861 caaaaaataa aaactgattt agtttcgttg caggaaaatg aagatcatga agtggtcgcc
     4921 attgatatcc agcttcatgc cacaacgttg atagaagacc tgttgcgatt attaaaaggt
     4981 atggcagggg tgaaaggcgt ttcaattagc taatctgtgt ggcagcgtag ccagactcac
     5041 cgtaagcctg aaattcacac cagataatca atatgctgat ggcgtaccag ctcattgatc
     5101 cgcttaagat tgaggatcat catgatattg tgtttatggc agtagaacgt ttttagcgat
     5161 attttcagtt tctccagaat atcttcagtt gtcatttgat tcgcaatgca gctcagaacc
     5221 tgatgttgct tgcgcgaaag tgacacattc gcttcaggca cagagcagta actgccattg
     5281 atgagtgcga caaaatcaat gatagtgata gtccgcggaa caaaggtcac cggtactttc
     5341 cccagacagc gtttaatatt aaagggggag aaaacaatga catggacagg ttttaatcgt
     5401 tcaatttgag tgagaaaatt agaatgaaat acattattca ttaatgtatc aataatgacg
     5461 aaggcattct gatgcaacat tacgtcgatc tcttcttcgt tctgtatatg aacgacatta
     5521 cctttactca gaatgttttt catcgcggtg aagaacatcg tatccctggt aattataaga
     5581 aacataaccg actcctggtg cggacattaa ctttataatc agtttgccaa aggtaattaa
     5641 caggacaggg cttataataa taatagttag cttactgtat attaatttta tgtaatacat
     5701 gatgcagatg ctgagaaagc atctgcatct ttcggtggtg ttatttgacc agctcaggtg
     5761 ttacctgact caccgcattg gtgtagtaag gcgcacccca gcccggttcc ggccagaatg
     5821 caccatagcc gtaatcccac aggttatagg tggtattaac cacttctctc aaatgccaca
     5881 cctggatgcc ctgcatattc acttccagga agttatacgg gactttattg ataaagcctg
     5941 gttgttcacc ctgaatggta ccgaggatgg taacaaagtg attacgatag ttcactggat
     6001 caaggaagcc gctttgtctg gcgagcagtc ggccctgata gttggcttca atatcaggct
     6061 tcgcatagct atccaacggt aatacagcga tttctaacaa cgtatccgtt ttgccattga
     6121 taacgttgat aaccttccca ccaaagcgcg cttgttgacc aacatataac cccggctggt
     6181 tatgaacagc aacaaaactt ttttgaatat caggttggtt attgcctttg atattttgcg
     6241 gaattgaact acatgcggca agcaaaaatg aaaggctgag gatgagtgca ccttttgtca
     6301 tgttcatgtt actatcctta tcaacaaata ttaaaaccat aatagatatt tctttatagt
     6361 tttcatctga ttctgaggtg atatgcctga tagtaacgta aacctgtctg atgtttatat
     6421 cccttctgtt tagttcaatt ccgttatttt ggggcgtggt atgagta
//
LOCUS       DAZPFP010000194         6282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPFP010000194 DAZPFP010000000
VERSION     DAZPFP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.197"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUR4_003532"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUR4_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1456953.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUR4_003533"
     CDS             2219..2767
                     /locus_tag="ACHUR4_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1456954.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUR4_003534"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUR4_003534"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1456955.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUR4_003535"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUR4_003535"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1456956.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUR4_003536"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUR4_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1456957.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1572 a         1546 c         1600 g         1564 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tg
//
LOCUS       DAZPFP010000195         6141 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPFP010000195 DAZPFP010000000
VERSION     DAZPFP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.198"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            157..504
                     /locus_tag="ACHUR4_003537"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHUR4_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHUR4_003538"
     CDS             557..952
                     /locus_tag="ACHUR4_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1456958.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUR4_003539"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUR4_003539"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1456959.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUR4_003540"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUR4_003540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1456960.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUR4_003541"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUR4_003541"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1456961.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..>6141)
                     /locus_tag="ACHUR4_003542"
     CDS             complement(5323..>6141)
                     /locus_tag="ACHUR4_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1456962.1"
                     /translation="FVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAM
                     DIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNG
                     AHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLA
                     LATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTY
                     CAGCHASIAADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1365 a         1618 c         1491 g         1667 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa a
//
LOCUS       DAZPFP010000196         6083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPFP010000196 DAZPFP010000000
VERSION     DAZPFP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.199"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHUR4_003543"
     CDS             <1..378
                     /locus_tag="ACHUR4_003543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456963.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUR4_003544"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUR4_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1456964.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUR4_003545"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUR4_003545"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1456965.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHUR4_003546"
     CDS             complement(1787..2476)
                     /locus_tag="ACHUR4_003546"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1456966.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUR4_003547"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUR4_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1456967.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUR4_003548"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUR4_003548"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1456968.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUR4_003549"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUR4_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1456969.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1524 a         1664 c         1534 g         1361 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aac
//
LOCUS       DAZPFP010000197         6076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPFP010000197 DAZPFP010000000
VERSION     DAZPFP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.200"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUR4_003550"
     CDS             complement(<1..143)
                     /locus_tag="ACHUR4_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR1456970.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHUR4_003551"
     CDS             379..651
                     /locus_tag="ACHUR4_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1456971.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUR4_003552"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUR4_003552"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1456972.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHUR4_003553"
     CDS             1111..1749
                     /locus_tag="ACHUR4_003553"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1456973.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUR4_003554"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUR4_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHUR4_003555"
     CDS             complement(3132..3785)
                     /locus_tag="ACHUR4_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1456974.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHUR4_003556"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHUR4_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6076)
                     /locus_tag="ACHUR4_003557"
                     /pseudo
     CDS             complement(5229..6076)
                     /locus_tag="ACHUR4_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1623 c         1607 g         1419 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct ctttta
//
LOCUS       DAZPFP010000198         6050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPFP010000198 DAZPFP010000000
VERSION     DAZPFP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.201"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            213..1334
                     /gene="ompC"
                     /locus_tag="ACHUR4_003558"
     CDS             213..1334
                     /gene="ompC"
                     /locus_tag="ACHUR4_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1456975.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1446..2501
                     /gene="apbE"
                     /locus_tag="ACHUR4_003559"
     CDS             1446..2501
                     /gene="apbE"
                     /locus_tag="ACHUR4_003559"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1456976.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2575..3639
                     /gene="ada"
                     /locus_tag="ACHUR4_003560"
     CDS             2575..3639
                     /gene="ada"
                     /locus_tag="ACHUR4_003560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1456977.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3639..4289
                     /gene="alkB"
                     /locus_tag="ACHUR4_003561"
     CDS             3639..4289
                     /gene="alkB"
                     /locus_tag="ACHUR4_003561"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1456978.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4365..6008
                     /gene="yojI"
                     /locus_tag="ACHUR4_003562"
     CDS             4365..6008
                     /gene="yojI"
                     /locus_tag="ACHUR4_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1456979.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1435 a         1518 c         1646 g         1451 t
ORIGIN      
        1 cattccgcaa taatcttaaa aagttccctt gtatttacat tttgaaacat ctatagcgat
       61 aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt ggattattct gcatttttgg
      121 ggagaatgga cttgccgact gattaatgag ggttaatcag tatgcagtgg cataaaaaag
      181 caaataaagg catataacag agggttaata acatgaaagt taaagtactg tccctcctgg
      241 tcccagctct gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca
      301 acaaattaga tctgtacggt aaagtagacg gcctgcacta tttctctgac gacaagtctg
      361 tagatggcga ccagacctac atgcgtcttg gcttcaaagg tgaaactcag gttactgacc
      421 agctgaccgg ttacggccag tgggaatatc agatccaggg caacagcgct gaaaacgaaa
      481 acaactcctg gacccgtgtg gcattcgcag gtctgaaatt ccaggatgta ggttctttcg
      541 actacggtcg taactacggc gtagtttacg acgtaacttc ctggaccgac gtactgccag
      601 aattcggtgg tgacacttac ggttctgaca acttcatgca gcagcgtggt aacggcttcg
      661 caacctaccg tagcaccgac ttctttggtc tggttgacgg tctgaacttc gctgttcagt
      721 atcagggcaa aaacggtagc ccggaaggtg aaggcatgac caacaacggt cgtgaagctc
      781 tgcgtcagaa cggtgacggc gttggcggtt ctattactta tgattacgaa ggcttcggta
      841 tcggtgctgc agtttccagc tccaaacgta ctgatgacca gaacttcggt ctgaaccgtt
      901 atgatgagcg ttacatcggt aatggcgacc gcgctgaaac ctacaccggt ggtctgaaat
      961 acgacgcgaa caacatctac ctagctgctc agtacaccca gacctacaac gcaactcgcg
     1021 taggcaactt gggttgggcg aacaaagcac agaacttcga agctgttgct cagtaccagt
     1081 tcgacttcgg tctgcgtccg tccctggctt acctgcagtc taaaggtaaa aacctgggtg
     1141 tcatcaatgg tcgtaactac gacgacgaag atatcctgaa atatgttgat gttggtgcga
     1201 cctactactt caacaaaaac atgtccacct acgttgacta caaaatcaac ctgctggacg
     1261 acaaccagtt cactcgtgac gctggcatca acactgataa catcgtagct ctgggtctgg
     1321 tttaccagtt ctaatctcga tggatatcga acaaagggcc tgcgggccct tttttcattg
     1381 ttttcagcgt acaaacacag ttttttggtg tactcttgcg accgttcgca tgaggataat
     1441 cacgtatgga aataagcttt acccgcgtgg cactgctggt tgccgcgctc ttctttgttg
     1501 gttgcgatca aaaaccacaa cccgccaaaa cccacgctac tgaagttacc gttcttgaag
     1561 gcaaaaccat gggtaccttc tggcgtgcca gcatcccggg cattgatgcc aaacgcagtg
     1621 ccgaacttaa agaaaagatt cagacccagc tggacgccga cgatcagctg ctttcgacct
     1681 ataaaaaaga ttccgcgctg atgcgcttta acgactcgca aagtttgtcg ccgtggccgg
     1741 taagtgaagc gatggccgat atcgtcacca cctcgctgcg cattggggca aggaccgatg
     1801 gtgcgatgga tattaccgtc gggccgctgg tgaatctgtg gggctttggc ccggaacaac
     1861 agccggttca aattccgagc caggaacaga tcgatgcgat gaaagccaaa accggcttac
     1921 agcacctgac ggtcatcaat cagtcgcatc agcaatatct gcaaaaagac ctgccggatt
     1981 tatatgtcga tctctccacc gtcggtgaag gttatgcggc ggatcacctg gcacgcttga
     2041 tggagcagga agggatttcc cgctatctgg tgtcggtggg cggcgcgctg aacagccgtg
     2101 gtatgaacgg tgaaggccag ccgtggcggg tagcgattca aaaaccaacc gataaagaaa
     2161 acgcggttca ggctgtggtg gatatcaacg gtcatgggat tagcacctct ggcagctacc
     2221 gtaactatta cgaactggac ggcaaacgtc tttcccatgt tatcgatccg caaaccgggc
     2281 gtcccatcga acacaatctg gtatccgtga cggtgattgc tccgacggcg ctggaagccg
     2341 atgcctggga tactggcttg atggtactcg ggccggagaa agccaaagag gttgttcgcc
     2401 gggaagggct ggcggtttat atgatcacca aagaaggcga tagctttaaa acctggatgt
     2461 caccacagtt taaaagcttc cttgtcagcg aaaaaaatta aagcgcaaga ttgttggttt
     2521 ttgcgtgatg gtgaccgggc agactaaagg ctatccttaa ccagggagct gattatgaaa
     2581 aacgccacat gcttaactga cgatcaacgc tggcaatctg tcttagcccg cgacccgaat
     2641 gccgacggcg aattcgtttt cgccgtgcgc accacgggta tcttttgccg tccttcttgc
     2701 cgcgccagac atgccttgcg ggaaaatgtc tccttctacg caaatgccag cgaggcactc
     2761 gccgccggat ttcgcccctg caaacgttgt cagccagaca aagccaatcc ccggcaacat
     2821 cggttggata aaatcaccca cgcgtgtcga ctgctggaac aggaaacgcc tgtaacgctg
     2881 gaagccttag ccgaccaggt ggcgatgagt ccatttcatc tacatcggtt gtttaaagct
     2941 actaccggaa tgacgcctaa agcctggcaa caggcctggc gcgctcgccg tttgcgcgaa
     3001 tcgctggcga aaggggagag cgtgacgacg tctattctta acgccggatt ccccgacagc
     3061 agcagttact accgcaaagc cgatgaaacg ctgggcatga cggctaaaca attccgtcac
     3121 ggtggcgaaa atctggcggt gcgttacgcg ctggctgatt gtgagctggg tcgttgcctg
     3181 gtggcagaaa gcgagcgggg gatttgcgcg atattgctgg gcgatgatga cgcgacatta
     3241 atcagcgagt tgcagcagat gtttcccgct gccgacaacg cgcctgccga tctgacgttt
     3301 cagcaacatg tgcgtgaagt gatcgccagc ctcaatcaac gcgatacgcc gctgacgtta
     3361 ccgctggata ttcgcggcac tgcttttcag caacaagtct ggcaggcact gcgcacgata
     3421 ccttgtggtg aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa accgaaagcg
     3481 gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg ctatcgtaat accctgtcat
     3541 cgggtggtcc gtggtgatgg cacactttcc ggttaccgct ggggcgtgtc gcgtaaagcg
     3601 caactgctgc gccgcgaagc tgaaaatgag gagaggtaat gttggatctg tttgccgatg
     3661 ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat tttacggcgt tttgctttta
     3721 atgctgcgga gcaactaatc cgcgatatta atgacgttgc cagccagtcg ccgtttcgcc
     3781 agatggtcgc tccaggcgga tataccatgt cggtggcgat gaccaactgt gggcatctgg
     3841 gctggacgac ccatcggcaa ggttatctct attcgcccat tgatccgcaa acaaataaac
     3901 cgtggcccgc tatgccacaa agctttcatg atttatgtca acgtgcggct acggcggcgg
     3961 gctatccaga tttccagcca gatgcttgtc ttatcaaccg ctacgctcct ggcgcgaaac
     4021 tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc gccaattgtt tctgtttctc
     4081 tgggcttacc cgcgattttt caatttggtg gcctgaaacg taatgatccg ctcaaacgtt
     4141 tgttgttgga acatggcgat gtggtggtat ggggcggtga atcgcggctg ttttatcacg
     4201 gtattcaacc gttgaaagcg gggtttcatc cactcaccac cgactgccgc tacaacctga
     4261 cattccgtca ggcaggtaaa aaagaataaa aataagaatt attattgctg tgcgcgcgaa
     4321 gatgtttaaa ctgcgggctg taattcattg tccgggtttt ctgcatggaa cttcttgtac
     4381 ttgtctggcg gcagtatcgc tggccattta tcagtgtgat ggcgctaagc ctcgccagtg
     4441 cggcattagg cattggctta attgctttta tcaatcagcg ccttatcgaa acggcggata
     4501 ccagtctgct ggtgttaccg gagtttctgg gattattgct gctgttgatg gcagtcactc
     4561 tcggatcgca actggcgctc accactttgg ggcatcactt cgtttaccga ctgcgtagtg
     4621 aatttatcaa gcggattctg gatacccacg tcgagcgcat tgaacaactc ggtagcgcct
     4681 cgttgctggc gggattaacc agcgatgtgc gcaatatcac cattgctttt gtgcgtctgc
     4741 cggaactggg gcaggggatc attctcacta tcggttcatc ggcgtatctg tggatgctgt
     4801 cgggcaaaat gttgctggta acggctatct ggatggcgat caccatctgg ggcggttttg
     4861 tgctggtggc gcgggtgtac aaacatatgg cgaccctacg tgaaaccgaa gacaagctgt
     4921 acacggattt tcaaactgta cttgaagggc gcaaagagct gactctgaac cgggaacgcg
     4981 ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca agagtatcgc catcatatta
     5041 ttcgcgcaga caccttccat cttagtgccg tgaactggtc aaacatcatg atgctgggcg
     5101 ctatcggcct ggtgttctgg atggcgaaca gtctcggttg ggctgatacc aacgttgctg
     5161 cgacctattc gttgacgctg ttattcctgc gtacgccgct gctttcggcg gttggcgcat
     5221 tgccgacgct gctgacggcg caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc
     5281 ctttcaaagc tgagtttccg cgcccgcagg cgtttcccaa ctggcaaacg ctggagctgc
     5341 gtaacgtgac gtttgcttat caggataacg cgttttccgt tggtccgatt aatctcacca
     5401 tcaaacgtgg cgagctgctg tttctgattg gcggtaacgg tagcggaaaa tcgacgctgg
     5461 cgatgttgtt gacgggcttg tatcagccac aaagcggcga aatcttgctg gatggcaaac
     5521 ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg tttaccgatg
     5581 tctggctgtt tgatcaactg ctggggccgg aggggcaacc cgctaacccg caactggttg
     5641 agaagtggct ggcgcagctg aaaatggctc ataagcttga gttaagcaac gggcgtattg
     5701 ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag
     5761 aagaacgcga tattatcctg ctggatgaat gggcggtgga tcaggatcca cacttccgtc
     5821 gtgagtttta tcaggtgttg ctgccgctga tgcaggagat gggtaaaact attttcgcta
     5881 tcagtcatga tgatcattac tttatccacg ccgaccgcct gctggaaatg cgcaatgggc
     5941 aacttagcga gctgacgggc gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga
     6001 cggcataata ttacgacgct gacctggcgg actgctgccg tcaggtcaat
//
LOCUS       DAZPFP010000199         5778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPFP010000199 DAZPFP010000000
VERSION     DAZPFP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.202"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(208..1920)
                     /locus_tag="ACHUR4_003563"
     CDS             complement(208..1920)
                     /locus_tag="ACHUR4_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR1456980.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            1985..2443
                     /locus_tag="ACHUR4_003564"
     CDS             1985..2443
                     /locus_tag="ACHUR4_003564"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1456981.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            2575..2835
                     /locus_tag="ACHUR4_003565"
     CDS             2575..2835
                     /locus_tag="ACHUR4_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR1456982.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(2925..4922)
                     /locus_tag="ACHUR4_003566"
     CDS             complement(2925..4922)
                     /locus_tag="ACHUR4_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1456983.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(4922..>5778)
                     /locus_tag="ACHUR4_003567"
     CDS             complement(4922..>5778)
                     /locus_tag="ACHUR4_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1456984.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1482 a         1411 c         1283 g         1602 t
ORIGIN      
        1 aaattaaact tttttataac ccctcttcct gacatgaact ggtctcaagg agcagaatta
       61 ataaataaag caaaacatgg agaagccgtc tcatttacag tgctccctcc atggttaact
      121 cttgatttaa taaaaaaata aaacttcaca ttaataaata acaatgaatc cgcctcccta
      181 cgtattcccg tagggaggct gtcgtgttta tagtagcctg gtaatgctaa atgcaaacga
      241 actagagatt cctgctccgc taagcgtatt gcgcagggtc accctaaatg tatctacgcc
      301 aatataagta acatcacaac taactgaatc aggcgcagat ataggtgtta tctgcacaat
      361 ataaccgcta gcaactgctg cgagcggatg agaaaagcta acctcataca cacctgtagc
      421 aaccacgtta accgatgaaa caccagtagc aacgtcgata ctaccattgg cgttaatttt
      481 tcccgagcgt gtgactgaca atgcagaagc cgatgttgcc ttactggttg tttctgtaaa
      541 aacacaccca atgtcaacta cctcgcaatt agctcctgtt atccagtatg gtcgatctgc
      601 acttggctga taatcccctg cgctctggaa cgttgtgccg ttgagtatga ctgtaacttt
      661 tcccccacct gagcttgtta cttgaatatt agtgtgcgtg tatcttgcac tgctcacgcg
      721 atgaaaattt ccaccgttta taaccacagt tacagggcgc gtcccagtat tatctatagc
      781 aaggtctgcc cccccggcat tagcctcaaa gtatgggttg tttaatacca aagcgcacga
      841 accgttaagg ccatttatgg ctaattgcgc accacctgta ctggaatccc cctgagtacc
      901 atttccttca caagtcaggc tattgatagt tgtaccagca ccccagcggt ccccgaggat
      961 accccattca ctgttctgac tgcacgtaag cctatttgca gttatagcgt tcagttcaga
     1021 atgatttacg gagttggtag cacgccaacc gatacgatta ctctgcaagt acacgttcgt
     1081 catgtcgctc gtgagcatcg agtcagtgcg taacccttcc ccgtcagggt ggcggcagat
     1141 aacaatgttt tcaagacgag tatgtgcttt attctgcacc agcataccgt aacttccggg
     1201 attccctttc agcgtcatgt tgccgatgta gtcatatgca gccaatctct gactgttaaa
     1261 cgctgcgtca cctagcattt ttacgttaaa atcagtagct ctacattcca gtattgtctc
     1321 agccatgttc tcgcctgagt aaaaacagcg gacacccgga gcacctacaa agtcggcagg
     1381 gtaatcatca taattaagta ccagaggagt ggtaacatca tacacccctt gctttatttt
     1441 taatgttcca ccgaaaggcc acgatgtaag cgggtcttga tatatttcat tttttattgc
     1501 aaatagctca tcttgaagat ttttcccgct attagtgcca atgtaagtag ccccttcatc
     1561 agattgaagt aactgcttta actgatccgg gtcatacttc agcaaattcg gaaaatagaa
     1621 ctgttgcgaa ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt
     1681 ggcaatctgc ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac
     1741 agggacgtga gatccgtctt cattctctac ataaacctga atctggtttt caggatttac
     1801 cgggtcagtg tcaattttcc cgatataaat ttttccattg gcatttgctt taaacgagcg
     1861 agctaaagta aataactgac ttggttgtga aacaacaacg ttgtataatt gttctggcat
     1921 aataaacatt ccggagaaaa taatgagtaa gagacgtcac gaggtagtaa ctcgggagcg
     1981 ccttatggag gttctctatt acaataaaga aacaggaatt ttcacttgga aaaagaaatt
     2041 aagcgccaga ggtgctgttg ggaaagttgc tggcacaatt tcttatggat ataacgcaat
     2101 taatattgat ggcgtaagat attttgcaca tagacttgcc tggctgtatg tatatggcga
     2161 atggcccaaa caagagattg accacattga tagaaacaga agaaataacg caatctcaaa
     2221 ccttagggat gtcagcagag tcgtaaatgc attaaatgtc ggacctcgaa atagcaatgc
     2281 tggaatcaag ggggtaactt tctgtcaagc cagaaatcaa tggcaagctc agataaatgt
     2341 ctcaggtaaa aacataaccc ttgggagatt caacaccatt gatgaagcgg ctatagctta
     2401 taaggcagca aacatggttg ctgaccatct gttgagtagg tgatatctga catttcattg
     2461 ctccagacga atgatatgat gcaaccatga tgtgattgca taccgaaatg gtactattga
     2521 gtatttatcc agtaagttac gatgccattc cacccaattg gtgaggcatc aaggatgtac
     2581 agcaaatacg acgaggcgca gtttcacttg agacttccgc atgaactcca cgcgaaaatt
     2641 aagcagcgtg cgaagatgaa taacaggtct ctgaactcag agataattgc agcgattgaa
     2701 gaatcactgg ataaacaaaa ctctgcatca gtttacattg acgatgcaga gcgtatggca
     2761 gaacaacaat ccgatatggt taagaaaatt gtctttgata cgctcaaaga gctatataaa
     2821 aaagacagca gctaaccatc agttacggag gatttatgca aagagatatg ctgaatattg
     2881 cgttctacat atttggtttt tgcacgttcc tggtgtttgc gaagctattc tgacaacgca
     2941 tcagacttgg caccctgagt cagggcgtta atggcctttt gcgcctgctg catggctttc
     3001 tcaaacgctg ttgatccgcg tggggtattt gccattcgga gcattgcatt tctgaatggc
     3061 tcgctctcat aggcgcgagt aagaagtccg tagcttaccg ctgcgccagt tgtcgccggg
     3121 ttcattgccg tcccataccc gataatgaac gggatggttt gctgccctgt tggtgttgtt
     3181 actgccgctt ttgctgcctg ctgagtggac tgaaggtaat ttttcaatcc tttcagataa
     3241 gcagcgtcct gccccttaaa tgtgatgcca gtctggtttt gcaggatgtt aagctgccga
     3301 aggaactggt caggggatcc gccagatttc tccatcgcct ttccaatgat gccattgcgc
     3361 atttgcaccc tgccaacacg accaactgag ttatacagcg tcttaatttc cgatttgttc
     3421 ttgctgaata gcatgttgtt gacaacttcc ggcgtcaggt cgcctttcat gagaacattc
     3481 ttcagcctgg tattctttag tttcgccgct tcgtcagcgt agacggcatt ggcctgctga
     3541 tatttacgga gagtatcgtt gccaagattc tgaccaatgg caccattgat atcgtcggtc
     3601 atcgccttgt aaacgcgctg aatggcagca tcggaacggt ttggtaacac tggtcgctca
     3661 cccttcacgt ccattctgaa ctgactgcgc aggtcgctta attgcttcaa atccagattt
     3721 accggacctt cagggccagc attgcgaaca agctcatcac gatatgactg aagttttgaa
     3781 attgtctcgt tatcagccac cttaccaagc ttctgcaggt tagatatttc tgtatcaatc
     3841 tgctgaattg ctcgcgcagg ctgaatgttt actccagcca tagcattctg aacctgctcc
     3901 agtcgattac cggcggcacg acgaattcct gatgttttcg ctttaaggct gtcaataaca
     3961 accgctggat catactcccc gaatttatca gcaaatctct gcaccaactg gcttctcgct
     4021 tcctgttgcg ttgctctcat tccgcttgtg ccagccagag ggatattttc tgctgtagtc
     4081 tgcgccattt ttccgacgcg ggaagttggt tgtaacaggt ctgtggtgtg cagaggaact
     4141 ccttcacgct ctgcaaatct gatagcctgc tgagcttctg gcgcgatagc accacgaacg
     4201 ccacgataag cagcacctaa tccacgtccg gcagcgttaa tagcaccgcc agcaagtaca
     4261 ccaacgccta aatcggtggc gagtgcttcc gcatcatctt tcgcactatt tgcagcaagt
     4321 gatccaactg cgttttcagc gagaaggcga gttgcaccct gagcaattcg accagcaagt
     4381 gttggtgcct gtgttgccgc tctctcaacg ccggcaggag tgaggtaagg caatgcttca
     4441 gcaaataccc tgccctctgt cgtttgtgga gtcagcgcac cttgctgaag gccaaagtcc
     4501 tgctctaatc catgcgttgt tactcgtggc gctggttgat atgttccatc gccaatgccg
     4561 agtttaccgc cagcccatgc agccgcgctt gttacagcat cggcaaccga tgcaggtatg
     4621 tttgccacgt tcacgccagc ctgcaccagt ccgcgaccag tctcttttac tgcttcgcca
     4681 agatcagaca taaatccact ttgctgtggt tgttgctgtg ctactggttg ctgtgtctcc
     4741 actggctgca cagatggcaa tggataggca gcatagaaag cttgcttagc ctgctctgca
     4801 ttttctccgg cttgcggggc aacgacttca ttgaagtatt gctcctgagc ctgcgctttt
     4861 tgttctggtg ctaacgcctg atactgtgga gatgcgataa catctttcca tgctttagcc
     4921 attaatcacc ccatagtgaa gaaaaattac tgccagcagg agattgttgc cctggcgtat
     4981 tctgcaccgg ctcctggtaa tcaaactgtt ttttaacagt gctcaacttg ctttcaagct
     5041 gatttcgaat ctttccgata gagtcacgaa aagccttttc actcattttg ggacttagag
     5101 caccaaccgc atcggataat tttttaccct cagcatctga aagagccccc atccccttca
     5161 gggactgcac catagggagg aaagtttgag ctttaaaggt gtcgagtctt gcttcaaagt
     5221 tagccgcatc agatccagga actgtcggaa acgctgagcg aataccaact gcttttgaaa
     5281 gtccagggct ttgctctatc tcgttgagag aatcaagcgc agtgctgaac gtatcaacag
     5341 cgccctgagc ggcggcctgt ctgtcagcac gagctatttc agccttttgc cgaacatctg
     5401 cctgtttctg ctttagctct tcaagcttta actgattgct ttctctggct atctgtctgt
     5461 ccagagcctt ttcttgtaat tctgctcttt gtatttcgcg ggaaagagca gcattctgtg
     5521 cgctgatgtt ctggccacgt atctggatgt cctgacctcg tgctgttagt gcctcgccag
     5581 cctgattgct gcggattgtc tctgccagcc tacctcggtc aatctcacga ccagccatct
     5641 tgtcctgaac attgaagtaa tcaatcggac caagagcagc catcccaagg tgatcaacaa
     5701 actcaccaaa tcctgaagga ttctgctgat acatctgagc aacgttgtta ggatcaacgc
     5761 cgacgcgagt cagttcct
//
LOCUS       DAZPFP010000200         5751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPFP010000200 DAZPFP010000000
VERSION     DAZPFP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.203"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            129..1037
                     /locus_tag="ACHUR4_003568"
     CDS             129..1037
                     /locus_tag="ACHUR4_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR1456985.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1083..3074)
                     /gene="tkt"
                     /locus_tag="ACHUR4_003569"
     CDS             complement(1083..3074)
                     /gene="tkt"
                     /locus_tag="ACHUR4_003569"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1456986.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3352..4110
                     /gene="loiP"
                     /locus_tag="ACHUR4_003570"
                     /gene_synonym="yggG"
     CDS             3352..4110
                     /gene="loiP"
                     /locus_tag="ACHUR4_003570"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1456987.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4316..5236)
                     /gene="speB"
                     /locus_tag="ACHUR4_003571"
     CDS             complement(4316..5236)
                     /gene="speB"
                     /locus_tag="ACHUR4_003571"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1456988.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5372..>5751)
                     /locus_tag="ACHUR4_003572"
     CDS             complement(5372..>5751)
                     /locus_tag="ACHUR4_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456989.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1383 a         1533 c         1516 g         1319 t
ORIGIN      
        1 gaaaacatac ttttctaatg caatgttttt tcgctatacc cctttataga ttctgaattc
       61 tcacctgtcg gctgttttct cctcatctca tgtgacatat tcccatactt tactcaagga
      121 agattgtcat gaaagcacct gtttcattaa cctcatttcg cccacaaaaa tctcttgcta
      181 tcgccattgg cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac accgttaatg
      241 aaggtgaacg cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc gaaccggggc
      301 tgggttttaa gatccctttt atggaaagcg tggagaaaat ttccacgcgt aatcaggctg
      361 tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa atgacagtat
      421 cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc tacaacacta
      481 ttgaagcatt aaaagatcgt ctaattgtcc gtcagttgcc aactcagctg gagaacattt
      541 tcggccaata taccgccatc agcgccgtac aggatcgtac aaaattggtt caggatctgc
      601 aaaatgccat gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc cagattgaaa
      661 atattgattt ttctgacgct tatgaaaagt cgattgaaaa ccgaatgaaa gcagaagtcg
      721 ccattgccac gcgtaagcaa aatctggaaa cagagaaaat ccaggcgcaa atagcggtaa
      781 ctcaggccca ggcagaagca gacagtaaac tggcggcagc caaagcagaa gcggaaacta
      841 ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg gctgaagcag
      901 aagcaatccg cctgcgcggt gaagctctgc gggataatcc cggacttgta gccctcacca
      961 ctgccgaacg ttgggacggt aaactgcccg ataccatgat ccccggcagc acagttccgt
     1021 ttatctctac taagtaaaaa aagggccgct ttcgcggtcc ttctcacaaa acgtcttact
     1081 gattacagca gcgcttttgc tttcgcaaca acgttatcaa cagtgaagcc gaactcttca
     1141 aacagcagct ctgccggagc agattcaccg aaggtggtca tgccgacgat agcgccgttc
     1201 aggccaacat acttgtacca gtagtcagca atacccgctt ctaccgcaac gcgtgcagta
     1261 accgctttcg gcagtacgga ttcacggtaa gccgcatcct gcttgtcaaa tgcgtcggta
     1321 gacggcatgg acaccacgcg cgctttcacg ccttcggcag tcagtttttc gtaggcagca
     1381 accgccagtt caacttcaga accggtggcg atgaagatca gttccggctg accagcacag
     1441 tctttcagca cataaccacc gcgcgcgatg tttgccagct gctcttcagt acgttcctgc
     1501 tgcgccaggt tctgacggga gaggataagc gcagtcgggc cgtcctgacg ctcaacgccg
     1561 tatttccacg cgaccgcgga ttcaacctgg tcacacggac gccatgtaga catgttcggg
     1621 gtcacgcgca gagaagcgac ctgctctacc ggctggtgag tcgggccatc ttcgcccaga
     1681 ccgatggagt cgtgggtgta aaccatcacc tggcgctgtt tcatcagcgc agccatacgt
     1741 acggcgttac gtgcgtattc cacgaacatc aggaaggtgg aggtgtacgg caggaagcca
     1801 ccgtgcaggg agataccgtt agcaatcgcg gtcataccga actcgcgaac accgtagtgg
     1861 atgtagttac ctgcagcatc ttcgttgatt gctttagaac cagaccacag ggtcaggtta
     1921 gacggtgcca ggtcagcaga gccgccgagg aattcaggca acagcgggcc gaacgcttcg
     1981 atagcattct gcgacgcttt acggctggcg attttcgccg gattagcctg cagtttagcg
     2041 ataaactctt tcgctttggc gtcgaagtca gacggcattt cgcctttcat acggcgggta
     2101 aattcagccg cttcctgcgg ataagctttc gcgtaagccg caaacttctc attccatgca
     2161 gattctttcg cctggcctgc ttctttcgca tcccactgag catagatttc agacgggatt
     2221 tcgaacggtg catatttcca gcccagctgt tcgcgggtca gggcaatttc agcgtcgccc
     2281 agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg gggaaccgaa accgatgatg
     2341 gttttgcaca tcagcaggga cggtttgtca gtcactgcgc gcgcttcttc aactgcgcgt
     2401 ttgatggatg ccgcgtcatg accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg
     2461 aaacgcattg ctgtgtcgtc ggtgaaccag ccttcaacgt gaccatcgat agaaataccg
     2521 ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg tacccgccag agagcaaact
     2581 tcgtgggaga tgccttccat catgcagccg tcgcccatga aggcgtaggt gtagtggtcg
     2641 acaatgtcgt ggcccggacg gttaaactgc gccgccagcg ttttttctgc aatcgccata
     2701 ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg tggtttccac accagcggta
     2761 taacctactt ccggatggcc cggagtttta gagtgcagct gacggaagtt tttcagttct
     2821 tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg
     2881 ccgttggaca gcacgaagcg gtcacggtca gcccaggacg gattctgcgg gttgtgtttc
     2941 aggaaatcac gccacaggac ttcggcaatg tcagccatac ccataggggc acccgggtga
     3001 ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct
     3061 ttacgtgagg acattttgac tccagatcgg atgatgaagg gcacgccctt aacgacttga
     3121 cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca atttaccgca agccgcgcgt
     3181 catgtacatg gaacatcctt ttgccgcttc agaaatctct ggatcatgct cgcatgttgc
     3241 gcaatctgct cgcccgtccg ctgcgctttt ccttatactg agactgagcg tcgattcacc
     3301 tgcaaacggc gcatttttag aataatcctg accttgtgcg gaagagaaaa catgaaaatt
     3361 cgcgccttat tggtagcaat gagcgtggca acggtactga ccggttgcca gaatatggac
     3421 tccaacggac tgctctcatc aggagcggaa gcttttcagg cttacagttt gagtgatgcg
     3481 caggtgaaaa ccctgagcga tcaggcatgt caggagatgg acagcaaggc gacgattgcg
     3541 ccagccaata gcgaatacgc taaacgtctg acaactattg ccaatgcgct aggcaacaat
     3601 atcaacggtc agccggtgaa ttacaaagtg tatatggcga aggatgtgaa cgcctttgca
     3661 atggctaacg gctgtatccg cgtctatagc gggctgatgg atatgatgac agataacgaa
     3721 gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt taggccatgt gaagaaaggg
     3781 atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg cggcctctgc gggcgggatt
     3841 gtcggcagtt tatctcaatc acaacttggt gatctgggcg agaaattagt caattcgcaa
     3901 ttctcccagc gccaggaagc agaagccgat gattattctt acgatcttct gcaccaacgc
     3961 ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac tggcgaagct ggaagaaggt
     4021 cgtcaaagct caatgtttga cgaccatcct gcatccgccg aacgtgccca gcatattcgc
     4081 gatcgcatca gcgcggatgg gattaagtaa agcctagtgg tgtcgaacgt aggtcagata
     4141 aggcgttcac gccgcatctg acattaatgg cacatttgtt accttgtgcg cattgccgga
     4201 tgcgatgctg gcgcatctta tccggcctac gggtgccgaa cgtaggtcgg ataaggcgtt
     4261 cacgccgcat ccgacattaa tggcacgttt tacccgtgcg catcgcatct ggtgcttact
     4321 cgcccttttt cgccgcctga atatacagca tctccagcgc cagcgtcgcc gcagccagag
     4381 cggtgatttc cgactgatcg tatgccggag ccacttccac tacgtccatc ccaacgatgt
     4441 tgagatcttt caggccgcgt accagtttaa tggcgcgatc ggaggtcagg ccgccaatca
     4501 ctggcgtacc ggttcccggc gcgaaagcag gatccaggca gtcgatatca aaagtcaggt
     4561 aaaccggcat atcacccaca atctgtttca cctgggcgat aacgtcatcc acgctgcgat
     4621 cgttcacctg gcaggcgtcc agcacggtaa agccgttgtc tttatcaaac tcggtacgaa
     4681 taccaatctg cacggaatga ttcgggtcga tcagaccttc tttcggcgcg gtatagaaca
     4741 tagtgccgtg gtcaaattca caaccgttcg cataggtatc ggtgtgggcg tcaaagtgta
     4801 ccagcgccat tttgccgaaa tgcttcgcat gagcacgcag cagcggcagc gtaacaaagt
     4861 ggtcaccacc gaaggagagc atacgcttac cggcagccag cagcttctcg gcgtgcgcct
     4921 gcagcttttc gctcatctca cgggcatcgc caaaggcata taccagatcg ccgcagtcca
     4981 cgacgttcag acgctcacgc atgtcgaaat tccacgggaa gcggttgtgt tcccaggcca
     5041 gattcgtcga aacctgacgg atcgctgccg gaccgtggcg accacccgca cgaccagaag
     5101 tggccatatc gaacggcacg ccagtaatca cccagtctgc atcgctgtca tacggctgga
     5161 agttcatcgg caggcgtaaa aaaccaaagg cattggaaac cagtgagtta tcgtattgat
     5221 gacctaaggt gctcatggat ggactcctat tacaaagtcg atataaaaaa acccttccgc
     5281 gtcgttaggc ccgacgagga agggttggat ttgcgaaaca caagtttgtg ctaattatcg
     5341 ccttaaatgg gcgaggcatc aagcgaggtt gctatttccg acacggtggc ggcgcatagc
     5401 cattgttatc tgccggaaag gcttgtccag agcacggcgg cagcgcgttt tgcgccgggc
     5461 cttgtaattc aaaggcggca aagccgatta ccccggtatt acgcacatcg ccttgcgccg
     5521 aattggctgc gtaggaatca gcaacgttgg cgaactgaaa agccgcttca gtatgtttat
     5581 ctttgcggaa tcctttaatc actaacgaat ctccggcatt cacaatatag ccgttgttat
     5641 tcagcgaacc ttgcttgccg ttcagcacat ccaggccatc agccgtagca acgatctcgt
     5701 agttggtgtt acgactgtaa ttacggacat ataattgata attcattccg a
//
LOCUS       DAZPFP010000201         5742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPFP010000201 DAZPFP010000000
VERSION     DAZPFP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.204"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..171
                     /locus_tag="ACHUR4_003573"
                     /pseudo
     CDS             1..171
                     /locus_tag="ACHUR4_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(487..825)
                     /locus_tag="ACHUR4_003574"
     CDS             complement(487..825)
                     /locus_tag="ACHUR4_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456990.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            747..1874
                     /gene="ltrA"
                     /locus_tag="ACHUR4_003575"
     CDS             747..1874
                     /gene="ltrA"
                     /locus_tag="ACHUR4_003575"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1456991.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1957..2136
                     /locus_tag="ACHUR4_003576"
                     /pseudo
     CDS             1957..2136
                     /locus_tag="ACHUR4_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2163..2648)
                     /locus_tag="ACHUR4_003577"
     CDS             complement(2163..2648)
                     /locus_tag="ACHUR4_003577"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1456992.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2904..3884)
                     /locus_tag="ACHUR4_003578"
     CDS             complement(2904..3884)
                     /locus_tag="ACHUR4_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR1456993.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3884..5083)
                     /locus_tag="ACHUR4_003579"
     CDS             complement(3884..5083)
                     /locus_tag="ACHUR4_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1456994.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5257..5391)
                     /locus_tag="ACHUR4_003580"
                     /pseudo
     CDS             complement(5257..5391)
                     /locus_tag="ACHUR4_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5405..>5742)
                     /locus_tag="ACHUR4_003581"
     CDS             complement(5405..>5742)
                     /locus_tag="ACHUR4_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1456995.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1483 t
ORIGIN      
        1 gctgaactgc tggaaccgct gtatgacatc ctgcgccagt atgtgctgat gcccggtaaa
       61 gtccatgccg atgatatccc cgttccggtc caggagccgg gcagcggtaa aacaaggact
      121 gcccggctgt gggtctacgt tcgtgatgac cgtaacgccg gttcggaaat tgcagcataa
      181 gttatccatg ctctgaggtg ggtagaggtg ggccctccgg tcagggttta caggagcaca
      241 gaccaaacga cccggtgctg cttcattcta aggaatgagg tggtgagcga ctcgggaaaa
      301 ggcagagatc tactgtcacg tgggttcggg aagctgaacg aaagtaaact gtccgatgaa
      361 gcatcgaaat cttagatgat gccaaaatgg ggccgtacag ccgtcccctg ataagcgcag
      421 aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat cccgggccag
      481 accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag gggcggaccc
      541 attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg ggatcacatt
      601 cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa aatcatttga
      661 aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca agggagcccc
      721 ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc aatctataca
      781 agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg gaaaaacgga
      841 tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc gatcgaattg
      901 ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc catgctgatt
      961 catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt gccatccggt
     1021 gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac catgtgagac
     1081 atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc atcctgtact
     1141 gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta ataacccgaa
     1201 cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg ttccttcact
     1261 atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag aggtacgctg
     1321 acgatattgt agtacattgt tcacgaatga gtgatgcaac aaggctaaag aacaggttat
     1381 cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat atcgcctaca
     1441 ttgacacgtt taaaaggaga aacgtcgcaa cgagtttcac ctttctcgga tatgacttca
     1501 aagtgcgtac gctgaagaat ttcaacggcg aacggtaccg aaaatgcatg ccgggtgcgt
     1561 caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata catcgctcaa
     1621 cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga ggctggatcg
     1681 agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt gcaatgcagt
     1741 cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag gctcagcgaa
     1801 agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat ttactacgtg
     1861 catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt tccgtgagca
     1921 gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag cgatgacgaa
     1981 catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggaaccagaa
     2041 aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg ggtacgtgat
     2101 ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt ctgacgagcc
     2161 gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta ttcagcaaga
     2221 agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg acgcatattc
     2281 aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg tatagttcca
     2341 tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg gcaccgctga
     2401 taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt cctgatttcc
     2461 cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt aggctgttgc
     2521 accagccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg tttgccatga
     2581 gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt gattttctgt
     2641 tcagacatgg tatcccgctt acttttgttg ttaaagttta tccatcatgg cgcaattgaa
     2701 ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg ctaagagaaa
     2761 ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg aaatactgtt
     2821 caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa aactggcgag
     2881 ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag tacaagagca
     2941 atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc atatgagaac
     3001 gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt ccataacaaa
     3061 gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc cttggttata
     3121 agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc agtaataagc
     3181 tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc cagcctcagt
     3241 cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga ctgaacagga
     3301 aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt caccttagct
     3361 gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc tttgagacgt
     3421 actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa atctttcgcc
     3481 agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg taatccaacc
     3541 ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat aagatcgcca
     3601 gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt gatgtctttt
     3661 agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac cacaaaagtt
     3721 tgagtatcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt tcggccagta
     3781 ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag gatatttgta
     3841 ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag ttggccctta
     3901 taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata cgtgcatttt
     3961 tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg gtatcaaacg
     4021 actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc atgtcaccac
     4081 cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc gccagtttcg
     4141 acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca ataatctgta
     4201 caagttcagg taaacgagcc aaatatttaa gcgtggaatg gaaatcaact tgtgctggcg
     4261 gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat gcgtccagat
     4321 gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca ataatattct
     4381 cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga gtatcccagt
     4441 ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca gggataactg
     4501 acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac atagcctgcg
     4561 gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata gtggctgatg
     4621 actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg tgaggatgag
     4681 cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg ccttttagac
     4741 taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga atgccacgat
     4801 gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg gttcctgctg
     4861 ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca tccacgcttg
     4921 cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga taaaaaacat
     4981 cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc aacattttat
     5041 ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct ccgtgctatg
     5101 taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg caagtacata
     5161 ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc ggaatgaata
     5221 ttactgtaag atagtatgcc tttccgggag aaagacgctg atttcccccg aataatccag
     5281 ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta tttcgcggga
     5341 aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg ggtagtgctt
     5401 ccaatcagta gaacatgtgc ttttcaatga aggagccgat gactttttcg tggatttcca
     5461 cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg cggagggtcc
     5521 ggttgttacg tgcaatacgt tgcgtgaaga ttttgccggt caggtgtttc tccttcggca
     5581 cttctctggc gtagctgccc cagtcatcgc tggtcaccat gccgatacag aacggagtca
     5641 acagggctaa cagttcccga caggtctcat cattccgtgg cccgaaagtg taagccaaca
     5701 caccgccagt tttagtgttg tacgcatacc agagccagtg tt
//
LOCUS       DAZPFP010000202         5738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPFP010000202 DAZPFP010000000
VERSION     DAZPFP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.205"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUR4_003582"
     CDS             <1..241
                     /locus_tag="ACHUR4_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1456996.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHUR4_003583"
     CDS             complement(238..1179)
                     /locus_tag="ACHUR4_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR1456997.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHUR4_003584"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHUR4_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHUR4_003585"
     CDS             complement(1684..2904)
                     /locus_tag="ACHUR4_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1456998.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHUR4_003586"
     CDS             complement(2908..3651)
                     /locus_tag="ACHUR4_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1456999.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHUR4_003587"
     CDS             complement(3542..5008)
                     /locus_tag="ACHUR4_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR1457000.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHUR4_003588"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHUR4_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZPFP010000203         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPFP010000203 DAZPFP010000000
VERSION     DAZPFP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.206"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUR4_003589"
     CDS             complement(<1..170)
                     /locus_tag="ACHUR4_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457001.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHUR4_003590"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHUR4_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1457002.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUR4_003591"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUR4_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUR4_003592"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUR4_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1457003.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUR4_003593"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUR4_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHUR4_003594"
     CDS             5484..>5702
                     /locus_tag="ACHUR4_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR1457004.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZPFP010000204         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPFP010000204 DAZPFP010000000
VERSION     DAZPFP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.207"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUR4_003595"
     CDS             complement(1..279)
                     /locus_tag="ACHUR4_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457005.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUR4_003596"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUR4_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1457006.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHUR4_003597"
     CDS             complement(1254..1670)
                     /locus_tag="ACHUR4_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457007.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHUR4_003598"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHUR4_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHUR4_003599"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHUR4_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHUR4_003600"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHUR4_003600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUR4_003601"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUR4_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1457008.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHUR4_003602"
     tRNA            3996..4071
                     /locus_tag="ACHUR4_003602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHUR4_003603"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHUR4_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZPFP010000205         5558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPFP010000205 DAZPFP010000000
VERSION     DAZPFP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.208"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..642
                     /locus_tag="ACHUR4_003604"
     CDS             <1..642
                     /locus_tag="ACHUR4_003604"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR1457009.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUR4_003605"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUR4_003605"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1457010.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUR4_003606"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUR4_003606"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1457011.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DAZPFP010000206         5509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPFP010000206 DAZPFP010000000
VERSION     DAZPFP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.209"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUR4_003607"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUR4_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1457012.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUR4_003608"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUR4_003608"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1457013.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHUR4_003609"
     CDS             complement(3736..4092)
                     /locus_tag="ACHUR4_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1457014.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHUR4_003610"
     CDS             complement(4096..4512)
                     /locus_tag="ACHUR4_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1457015.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUR4_003611"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUR4_003611"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1457016.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1291 a         1456 c         1444 g         1318 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtat
//
LOCUS       DAZPFP010000207         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPFP010000207 DAZPFP010000000
VERSION     DAZPFP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.210"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHUR4_003612"
     CDS             complement(<1..1630)
                     /locus_tag="ACHUR4_003612"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1457017.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHUR4_003613"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHUR4_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHUR4_003614"
     CDS             complement(2541..3146)
                     /locus_tag="ACHUR4_003614"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1457018.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHUR4_003615"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHUR4_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR1457019.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZPFP010000208         5450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPFP010000208 DAZPFP010000000
VERSION     DAZPFP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.211"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            494..1483
                     /gene="araF"
                     /locus_tag="ACHUR4_003616"
                     /pseudo
     CDS             494..1483
                     /gene="araF"
                     /locus_tag="ACHUR4_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1553..3067
                     /gene="araG"
                     /locus_tag="ACHUR4_003617"
     CDS             1553..3067
                     /gene="araG"
                     /locus_tag="ACHUR4_003617"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1457020.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3082..4068
                     /gene="araH"
                     /locus_tag="ACHUR4_003618"
     CDS             3082..4068
                     /gene="araH"
                     /locus_tag="ACHUR4_003618"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1457021.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4235..5035
                     /gene="otsB"
                     /locus_tag="ACHUR4_003619"
     CDS             4235..5035
                     /gene="otsB"
                     /locus_tag="ACHUR4_003619"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1457022.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5010..5450
                     /locus_tag="ACHUR4_003620"
                     /pseudo
     CDS             5010..5450
                     /locus_tag="ACHUR4_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1343 a         1267 c         1461 g         1379 t
ORIGIN      
        1 taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt taattaatga
       61 gcacagtgat aattaatggc gaaatgaatg acgcgcattt tccacatctt tgagttgtgg
      121 ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact ggttaaaaag
      181 aagacatccc gcatgggtac cgaagacaac aaggatttcc aggctaatct tatggattaa
      241 tctgctgtgc attcaacaat ttgtctgaca aattggcttt tccttatgtc ttttcccgct
      301 aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct atggagaatt
      361 aatttctcgc taaaactatg tcaacacggt cacttatctt ttagataaaa ggtaatgctt
      421 tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa cgacactaaa
      481 gctggagaga accatgcaca aatttactaa agccctggca gccattggtc tggcagccgt
      541 tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga agcaaccgga
      601 agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg atttagggtt
      661 tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga tcgacagcct
      721 ggcagccagt ggcgcaaaag gtttcgttat ttgtactccg gacccgaaac tcggctctgc
      781 catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gcagtggatg accagtttgt
      841 taacgccaaa ggtaagccaa tggataccgt tcctctggtg atgatggcgg cgactaaaat
      901 tggcgaacgt cagggctagg aactgtataa agagatgcag aaacgtggct gggatgtcaa
      961 agaaagcgca gtgatggcga ttaccgccaa cgaactggat accgcccgcc gccgtacaac
     1021 gggttctatg gacgcactga aagctgcagg attcccggaa aaacaaattt atcaggtacc
     1081 taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc tggttcaaca
     1141 tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtac ttggcggcgt
     1201 acgtgcaacc gaaggtcagg gctttaaagc agccgatatc atcggtattg gtattaacgg
     1261 tgtggatgca gtaagcgaac tgtctaaagc acaggcaacc ggcttctacg gttccctgct
     1321 gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact gggtagcaaa
     1381 agacgttgaa acgccaaaat ttaccgaagt tacagacgtg gtactgatca cgcgtgacaa
     1441 ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg gaaaaattcc
     1501 cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg ttatgcaaca
     1561 gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg ttaaggcgct
     1621 gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg gtgaaaatgg
     1681 cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa ccacgggttc
     1741 tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac ttaacgcggg
     1801 tgtggcgatt atttaccagg aactgcatct cgtgccggaa atgaccgtcg cggaaaacat
     1861 ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc tgaattatga
     1921 ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc cgctgaaata
     1981 tctcgccatt ggtcagtggc agatggttga aatcgccaaa gcgctggcgc gtaacgctaa
     2041 aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg gcaatctttt
     2101 ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt ctcaccgtat
     2161 ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac gttatgtcaa
     2221 aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga tggtcgggcg
     2281 cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc gcctgcgtct
     2341 tgatgctgtg aaagcactag gcgtgcgtac gccaataagt ctggcggttc gcagtggtga
     2401 aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga aaggcttgtt
     2461 tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga tcgatattcg
     2521 taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagctc gcaaagcgga
     2581 aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca gacgcaaaca
     2641 tgtgctcggc ggttgtgtaa tcaacaacag ttgggaagaa aacaatgccg atcagcacat
     2701 tcgttcgctc aacatcaaaa cgccgggcgc ggagcaactg atcatgaatc tctcaggcgg
     2761 aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg tcattttgct
     2821 ggatgaacct acgcgcggca ttgatgttgg cgctaagcac gaaatatata acgtaattta
     2881 tgcgctggcg gcgcagggcg tggcggtgct gtttgcctcc agcgacttac ctgaagtcct
     2941 cggcgttgcc gaccggattg tggtgatgcg ggaaggcgaa atcgccggtg aattgttaca
     3001 cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca gccaggcagt
     3061 tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg cgcacctaag
     3121 tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt gtttgcggtg
     3181 ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa tatgaaaggg
     3241 ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt ctgccttgct
     3301 tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt caccacggcg
     3361 gtggttatca acctgactga aagcctgtgg attggcgtgg cagcgggttt gttgctgggc
     3421 gttctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa tgctctgatc
     3481 acgacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc agacggtaaa
     3541 gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg gttcggtctg
     3601 cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgct gctgaataaa
     3661 accacctttg gtcgtaatac cctggcgatt ggcgggaacg aagaggctgc gcgtctggcg
     3721 ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct ggtatctgcg
     3781 atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac gtcgattggt
     3841 tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa aggtggcatc
     3901 ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga aaacgccatg
     3961 aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat cctgctggca
     4021 gcggtgatct tcgaccgtta caagcaaaaa gcgaagcgca ctgtctgatg cttttttcca
     4081 caacaactta acgttttttc ccaccacagc cagccgccac aacggttggc tgttcttcgt
     4141 tgcaaatggc gacctccgtc acactgtcta tacttacatg tctgtaaagc gcggtctgcg
     4201 caacacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa ccgaaacccc
     4261 tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg cggaaatcaa
     4321 accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac agctactggc
     4381 aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg agcttgacgc
     4441 actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc gccgtgacat
     4501 caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata ttagcgtgca
     4561 actgcatacg gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag ggatggcttt
     4621 tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat tagcgcaacg
     4681 tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg tcgagatcaa
     4741 accgagaggt accagtaaag gtgaagcgat tgcagccttt atgcaggaag ctccttttat
     4801 cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct tcgcagtcgt
     4861 taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg catcatggcg
     4921 actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccgccg cattacaaca
     4981 aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta tctaaccgga
     5041 ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc atactggggg
     5101 cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg aatgaggatc
     5161 agccgctaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac aggaccttga
     5221 cgaatactac aaccaattct ccaatgccgt tctctggccc gcttttcatt atcggctcga
     5281 tctggtgcaa tttcagcgtc ctgcctggga cggctatcta cgcgtaaatg cgttgctggc
     5341 agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg attatcacct
     5401 gttgcccttt gcgcatgaat tacgcaaacg tggggtgaat aatcgcattg
//
LOCUS       DAZPFP010000209         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPFP010000209 DAZPFP010000000
VERSION     DAZPFP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.212"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHUR4_003621"
     CDS             <1..497
                     /locus_tag="ACHUR4_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR1457023.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUR4_003622"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUR4_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHUR4_003623"
     CDS             2500..3114
                     /locus_tag="ACHUR4_003623"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1457024.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHUR4_003624"
     CDS             3114..3443
                     /locus_tag="ACHUR4_003624"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1457025.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUR4_003625"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUR4_003625"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1457026.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHUR4_003626"
     CDS             4529..4840
                     /locus_tag="ACHUR4_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1457027.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHUR4_003627"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHUR4_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZPFP010000210         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPFP010000210 DAZPFP010000000
VERSION     DAZPFP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.213"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHUR4_003628"
     CDS             complement(99..581)
                     /locus_tag="ACHUR4_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457028.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHUR4_003629"
     CDS             complement(649..2598)
                     /locus_tag="ACHUR4_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1457029.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHUR4_003630"
     CDS             complement(2683..3018)
                     /locus_tag="ACHUR4_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1457030.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHUR4_003631"
     CDS             complement(3018..3374)
                     /locus_tag="ACHUR4_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1457031.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHUR4_003632"
     CDS             complement(3374..4870)
                     /locus_tag="ACHUR4_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1457032.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHUR4_003633"
     CDS             complement(4867..5028)
                     /locus_tag="ACHUR4_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1457033.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHUR4_003634"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHUR4_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457034.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZPFP010000211         5339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPFP010000211 DAZPFP010000000
VERSION     DAZPFP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.214"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUR4_003635"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUR4_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1457035.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHUR4_003636"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHUR4_003636"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1457036.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUR4_003637"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUR4_003637"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1457037.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUR4_003638"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUR4_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1457038.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZPFP010000212         5337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPFP010000212 DAZPFP010000000
VERSION     DAZPFP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.215"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHUR4_003639"
     CDS             <1..171
                     /locus_tag="ACHUR4_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457039.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHUR4_003640"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHUR4_003640"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1457040.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUR4_003641"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUR4_003641"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1457041.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUR4_003642"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUR4_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1457042.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUR4_003643"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUR4_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1457043.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1317 a         1188 c         1399 g         1433 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatg
//
LOCUS       DAZPFP010000213         5304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPFP010000213 DAZPFP010000000
VERSION     DAZPFP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.216"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..4079
                     /gene="sepA"
                     /locus_tag="ACHUR4_003644"
     CDS             <1..4079
                     /gene="sepA"
                     /locus_tag="ACHUR4_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR1457044.1"
                     /translation="LKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVAALSLSS
                     AWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSS
                     ATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTR
                     RLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGG
                     FLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWV
                     LIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAANGTGTLT
                     QGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQ
                     TWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQ
                     QADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQ
                     AADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYDSSTKQY
                     FILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQLNGNMD
                     VNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQF
                     TLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVSSVEGSA
                     TATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGSFVNS
                     SLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLED
                     NASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSIT
                     GAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNTTFLMHT
                     NNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGF
                     SNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMG
                     DLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGALLTYTD
                     SNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLGTKDYSS
                     HSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIG
                     RTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTV
                     GMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            complement(4248..4862)
                     /gene="istB"
                     /locus_tag="ACHUR4_003645"
                     /pseudo
     CDS             complement(4248..4862)
                     /gene="istB"
                     /locus_tag="ACHUR4_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(4894..5304)
                     /locus_tag="ACHUR4_003646"
                     /pseudo
     CDS             complement(4894..5304)
                     /locus_tag="ACHUR4_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1383 a         1279 c         1361 g         1281 t
ORIGIN      
        1 atcttaagta ttgccatata accaaaagcc tgattgctgt ctccgaactg gcccgcaggg
       61 ttacctgcaa aagccatcgc agactttcac gtcgggttat tcttacgtct gttgcagctt
      121 tatcactttc ctcggcatgg ccagctctgt cagcaacggt cagtgcagag atcccttatc
      181 agatattccg cgactttgcc gagaataaag gtcagtttac gccggggacc acgaatattt
      241 ccatttacga caagcagggc aatctggtcg gtaaacttga taaagccccg atggctgatt
      301 tcagtagtgc cactattact accggtagcc tgcctccggg agaccataca ctttactccc
      361 ctcagtatgt ggttaccgcc aaacatgtca gcggttctga cacgatgagc ttcggatacg
      421 ccaaaaacac ctatactgcg gttggtacaa ataataatag cggtcttgat attaaaaccc
      481 gccgcctgag taaactggtg acagaggtgg cgccggcaga agtgtctgac atcggggctg
      541 tgagcggtgc ctatcaggcc ggcggacgct ttactgaatt ctatcgcctt ggggggggga
      601 tgcagtacgt caaagacaaa aatggtaatc gcacccaagt gtataccaat ggcggatttc
      661 ttgttggggg aaccgtcagc gcgctgaact cctataacaa cggacagatg attacagctc
      721 agaccggtga catttttaat cccgcgaatg gacctctggc taattatctg aatatggggg
      781 acagcggctc ccccctgttt gcttatgatt ccctgcaaaa aaaatgggta ctgattggcg
      841 tgctttcctc aggaactaac tatggaaata actgggtcgt caccactcag gatttccttg
      901 gtcagcaacc gcaaaatgat tttgacaaaa ctatagccta cacctctggt gaaggagtac
      961 tgcagtggaa atatgatgcg gctaatggca ccggcacact gactcaggga aacacgactt
     1021 gggatatgca tggaaagaaa ggaaatgatc tgaacgcagg aaaaaacctc ctgttcaccg
     1081 gcaataatgg cgaggtcgtt ctgcagaatt ccgttaatca gggagctggg tatctgcagt
     1141 ttgccggcga ttacagggtg tccgccctga acggccagac atggatgggg ggcgggatta
     1201 tcaccgataa aggaacccac gttctgtggc aggtgaacgg agttgccggt gataacctgc
     1261 ataaaactgg cgaaggtacc ctgacagtaa acgggaccgg tgtgaatgca ggcggactca
     1321 aggtggggga cggtaccgtt attctcaacc agcaggcgga tgctgacgga aaggtacagg
     1381 ctttcagctc tgttggtatt gccagcggtc gtccgacagt tgtgctttct gactcacagc
     1441 aagttaatcc ggataatatt tcgtggggat accgtggagg acgtctggaa ctgaatggta
     1501 ataatctgac ctttacccgt cttcaggcgg ctgactacgg ggccatcatt accaataaca
     1561 gtgagaaaaa atcaacggta acactcgacc ttcagacatt aaaagccagt gacatcaatg
     1621 taccggttaa tactgtcagt atttttgggg ggagaggagc gccgggagac ctatattatg
     1681 acagttctac caagcaatat ttcatcctga aagcgagttc atattccccg ttcttctccg
     1741 atctgaacaa cagcagtgtc tggcagaatg tcggaaaaga tcgtaataaa gccattgata
     1801 ctgtgaagca acaaaaaatt gaggccagca gccagcctta tatgtatcac ggccaactaa
     1861 acggcaatat ggatgtgaat attccgcagc tctcaggtaa ggatgtactg gctcttgatg
     1921 gttcagttaa cctgccagaa ggttcgataa ccaagaagtc cggcacgctg atattccagg
     1981 gtcatccagt tattcatgct ggaacgacga cttcttccag ccagagcgac tgggaaaccc
     2041 gtcagtttac gctggaaaaa ctgaaactcg atgcggcaac attccatctg tccagaaacg
     2101 gcaagatgca gggagatatt aacgccacca atggaagtac agtcattctg ggaagtagcc
     2161 gtgtctttac tgacaggagt gacggaaccg gtaatgcggt ctcctctgtt gaagggagtg
     2221 ccactgcaac cacagttggt gaccagagtg attacagcgg aaatgttacg ctggaaaata
     2281 aatcatccct gcaaatcatg gagagattca ccggtggtat tgaggcttat gatagcaccg
     2341 tgagtgtgac ctctcagaat gctgtttttg accgtgttgg cagctttgtc aacagcagcc
     2401 tgaccctcgg aaaaggagca aaacttacgg ctcagagcgg tattttcagc accggggctg
     2461 tggatgtaaa agaaaatgct tctctgaccc tgacggggat gccttctgcg cagaaacagg
     2521 ggtattactc acccgtaatt tctacaacag aagggattaa cctcgaagat aacgccagct
     2581 tttctgttaa aaatatgggc tatctgagtt cggatattca tgcaggaacc actgcggcaa
     2641 ccattaatct gggagacagt gatgctgatg cagggaagac ggactctccg ttattcagct
     2701 ccttaatgaa gggatataac gctgttctga gaggcagtat tacgggggca cagagtacgg
     2761 taaatatgat caatgctctg tggtactctg acggaaaatc agaggccggt gcactgaagg
     2821 ctaagggctc gcgaattgaa ctgggggacg ggaaacactt tgccacttta caagtaaaag
     2881 agctttctgc agataatacc acgttcctga tgcataccaa caacagccgg gctgaccagt
     2941 tgaatgtcac agacaaactg tcaggcagta ataatagcgt cctggttgac tttttaaaca
     3001 aaccagccag tgaaatgagc gtgacgttaa ttaccgcacc gaaagggagt gatgagaaaa
     3061 cgttcactgc cggtacgcag cagattggtt tcagtaacgt tacgccagta atcagcacgg
     3121 aaaaaacgga tgatgccaca aaatgggtgc tgacaggata tcagactacc gctgatgccg
     3181 gagcctcgaa agccgcaaaa gactttatgg catcaggtta taagtccttc cttacagagg
     3241 tcaataacct gaacaaacgt atgggtgacc tgcgggatac tcagggggat gccggtgtct
     3301 gggcacgcat aatgaatggt accggttcgg cagatggtga ctacagcgat aactacactc
     3361 acgttcagat tggtgtcgac agaaagcatg agctggacgg tgtggattta tttacggggg
     3421 cattgctgac ctatacggac agcaatgcaa gcagccacgc attcagtgga aaaaacaaat
     3481 ccgtgggtgg cggtctgtat gcctctgcac tctttaattc cggagcttat tttgacctga
     3541 ttggtaaata tctccatcat gataatcagc acacggcgaa ttttgcctca ctgggaacaa
     3601 aagactacag ctctcattcc tggtatgccg gtgctgaagt tggttatcgt taccacctga
     3661 cgaaagagtc ctgggtggag ccacagatag agctggttta cggttctgta tcaggaaaag
     3721 cttttagctg ggaagaccgg ggaatggctc tgagcatgaa agacaaggat tataacccac
     3781 tgattggccg tactggtgtt gacgtgggaa gagccttctc cggagacgac tggaaaatca
     3841 cagctcgagc cgggctgggt tatcagttcg acctgctggc gaacggagaa acggttctgc
     3901 aggatgcttc cggagagaaa cgtttcgaag gtgaaaaaga tagcaggatg ctgatgacgg
     3961 tagggatgaa tgcggaaatt aaggataata tgcgtttggg actggagctg gagaaatcag
     4021 cgttcgggaa atataatgtg gataatgcga taaacgccaa cttccgttat gttttctgac
     4081 gaatgggata ggtctgccat tcggcaggcc tttttctgaa agaactccgt gagcctgttt
     4141 aagattctgt gtaaatgcct tttctcagaa gtgaccgtcc aggtattaga cgtcaactag
     4201 ttttgccgac tggcgccaac tatactggcc gccccttcag tgcgacatca agtcacgctt
     4261 gttactgcct tcgctgtctt tttcctgtag ctttcccctt tcagttcgaa catatatccg
     4321 tgatggatca gtcggtctgc tgccgccacc gccatagtct catccacgaa gatgctgccc
     4381 cacatgctga acggatggtt actggttatc accaggctcc cacgttcata gcgatgcgct
     4441 attaactcga acagcacacc cgtttcggcg ctgtcgcgtt tgacatagcc aagatcatcc
     4501 accactatca cccggtagcg atccagtttc agtagcagct cattcagttt caactgagct
     4561 ctggctttac gtagttcctg caacagctct cctgcgctgt agaaccgggc ccggtagccc
     4621 tggcctacta cgccatccac gatcgctgcc gccagatggc ttttccccaa cccgctggcc
     4681 ccgaacagca gaacgttttc tccagcatca acccagtctg tcgtttcaca gagttgctgg
     4741 aactgagctc cattcagttc cggcaactga atgaagtcgt attcgcttag cgttttggca
     4801 actggcaacc gggcctcctt tttataacga cgcagttttt cactctcgcg ccacaggagt
     4861 tctgtaagcg tcaacggaag gccgtattga cgcttattta tcggtgagaa ctacgttcca
     4921 tggcagcagt tcgtcaactc ggttggaagg ccattccggc agtacgctca ggatatggcg
     4981 cagatacgct tctggatcga taccgttcaa ccgacagctc ccgattagtc cctacagcag
     5041 agctccgcga tcgcctccat gatcgctgcc gaagaacatg taattctttt tcccgagaca
     5101 gacggtacga agcgctcttt ctgctgtgtt attgtccgcc tccgccagac cgtcatcact
     5161 gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc ccagtctgga
     5221 ttttttcgac agcgtaccat tcttctccac catccattca ttcagcgacg tcagtaacgc
     5281 tttgcttcgc tgctgcctga cggc
//
LOCUS       DAZPFP010000214         5280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPFP010000214 DAZPFP010000000
VERSION     DAZPFP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.217"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUR4_003647"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUR4_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1457045.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2583
                     /locus_tag="ACHUR4_003648"
                     /pseudo
     CDS             1957..2583
                     /locus_tag="ACHUR4_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2782..3870
                     /gene="serC"
                     /locus_tag="ACHUR4_003649"
     CDS             2782..3870
                     /gene="serC"
                     /locus_tag="ACHUR4_003649"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1457046.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUR4_003650"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUR4_003650"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1457047.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1313 a         1263 c         1358 g         1346 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt cgctcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
     2101 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
     2161 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
     2221 gcctggtaat gtgatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
     2281 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
     2341 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
     2401 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
     2461 gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg ggaaaaacaa
     2521 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
     2581 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
     2641 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
     2701 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
     2761 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
     2821 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
     2881 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
     2941 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
     3001 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
     3061 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
     3121 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
     3181 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
     3241 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
     3301 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
     3361 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
     3421 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
     3481 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
     3541 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
     3601 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
     3661 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
     3721 cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt ggtcggcgga
     3781 atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct gacagacttc
     3841 atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc cccacagcca
     3901 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
     3961 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
     4021 cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
     4081 cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct atacgctttc
     4141 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
     4201 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
     4261 tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc gcccgattgg
     4321 tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
     4381 ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg ttgatggctc
     4441 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
     4501 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
     4561 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
     4621 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
     4681 ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
     4741 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
     4801 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
     4861 tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
     4921 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc gcctgtttgc
     4981 gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg attacattcg
     5041 tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
     5101 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
     5161 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
     5221 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
//
LOCUS       DAZPFP010000215         5278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPFP010000215 DAZPFP010000000
VERSION     DAZPFP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.218"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(511..887)
                     /gene="rnpB"
                     /locus_tag="ACHUR4_003651"
     ncRNA           complement(511..887)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUR4_003651"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(920..2065)
                     /gene="garK"
                     /locus_tag="ACHUR4_003652"
     CDS             complement(920..2065)
                     /gene="garK"
                     /locus_tag="ACHUR4_003652"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1457048.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2162..3052)
                     /gene="garR"
                     /locus_tag="ACHUR4_003653"
     CDS             complement(2162..3052)
                     /gene="garR"
                     /locus_tag="ACHUR4_003653"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1457049.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3082..3852)
                     /gene="garL"
                     /locus_tag="ACHUR4_003654"
                     /pseudo
     CDS             complement(3082..3852)
                     /gene="garL"
                     /locus_tag="ACHUR4_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3868..5202)
                     /gene="garP"
                     /locus_tag="ACHUR4_003655"
     CDS             complement(3868..5202)
                     /gene="garP"
                     /locus_tag="ACHUR4_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1457050.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1281 a         1494 c         1251 g         1252 t
ORIGIN      
        1 taaatgcaat atagacttaa taacttctta caaagtctat tattaatacc gtagatattc
       61 atcttaccct gtgcaccaaa agcaaaaact tatcgacgcg ggtttgaaac tgatggcaac
      121 cgcaaaaatg cctgatgcgc tacgcttatc aggcctacgc catctctgca atatattgaa
      181 tttgcgtgct tttgtaggca ggataaggcg ttcacgcagc atccggcatg aataaagcgc
      241 actcaacaat ctgaaacccg ccggaacggt ttattacgta catcaggtga aactgaccga
      301 taagccgctt tcttttgggt atagtgtcgt ggacagtcat tcatctttct gcccctccaa
      361 aagcaaaaac ccgccgaagc gggtttttac gtatatcagg tgaaactgac cgataagccg
      421 ctttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      481 aacccgccga agcgggtttt tacgtaaaac aggtgaaact gaccgataag ccgggttctg
      541 tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg
      601 ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg
      661 tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg
      721 atcccgcttg cacgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc
      781 cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc tccacccgtc
      841 tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata
      901 gagggtttgc gcgcccttgt caccccgtgt tgcgcattcc aatcgccagt gtcgcggcaa
      961 tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac
     1021 ctatgctggt caatacgctg aagaccgcat caatgccatg ctgatgtaca acgccaacat
     1081 catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg
     1141 cgacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga
     1201 tcaccagcgt acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa
     1261 taccactttt cagttccgca ccaagaaacg ccatcagcgc cgcacccata ccgcccgcag
     1321 ctcctgcgcc ggggacatct tttacatcaa catgcagcgc ttttttaatg acatcggcat
     1381 agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc
     1441 caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc
     1501 gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat
     1561 taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca
     1621 gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa
     1681 tgtttgtcgc gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg
     1741 tcacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt
     1801 caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca
     1861 gcggccctgt aacccaggcg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca
     1921 ccgttccttc gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt
     1981 cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag
     2041 agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt
     2101 ttctcttatt cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc
     2161 attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacaccagg gcgctatgat
     2221 ccgccgttcc taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga
     2281 gcggcagttg cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat
     2341 gcagatcaat acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat
     2401 ccagcacggt actgcccgcc agtccaccgc gaattgcctg atacaccagg tccgggttaa
     2461 cgcccgcttt agttgccagc gttaacgctt ctgacatcgc ggcaatattc agcgccacaa
     2521 tgacctgatt cgccagtttg gtgacgttac ctgcaccaat tgccccggta tgcaccacgg
     2581 aacccgccat tgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca
     2641 ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca
     2701 gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga
     2761 tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac
     2821 ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact
     2881 gttcagcgat cgctttagcc gtagaggctg tttctgcacc tgcagcaatc acgtcagcaa
     2941 tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac
     3001 tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca
     3061 tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttat gagtggcaga
     3121 gcggaagacg ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg
     3181 acgcgcatcg gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg cgctggcacg
     3241 gttaaaaatg tgctgaattg ctttttgtac atccgggtgt gatgcattgc cgagatggcc
     3301 taatgccgcg gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc
     3361 aatggcatcg acgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt
     3421 cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac
     3481 ggagacgccg cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc
     3541 ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt
     3601 aataatttcc ggctcgttgg tcggcactca cactactggc gcgctggcgc tgcctttcaa
     3661 ggccattaac tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac
     3721 cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga
     3781 ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac
     3841 atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc
     3901 aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc
     3961 tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga
     4021 ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc
     4081 ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc
     4141 catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc
     4201 cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt
     4261 gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg
     4321 aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat
     4381 cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac
     4441 gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc
     4501 tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa
     4561 cttcagctct tcctcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca
     4621 cagcgccgtc agcacaaaac aaatcacccc cataacggta aagacgtgct cccatcccca
     4681 ggcgaaagtc agccagccaa gcagaggcga aaagagcgcc agcgagaaat attgtgccga
     4741 gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg
     4801 ggcgttcgcc gggaaggatg gagcttccga gaatcccagc ataaagcgca taaagaacat
     4861 ggagatccct gcccaagcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag
     4921 cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca
     4981 gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcggagaaga tgtaacccat
     5041 cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc
     5101 acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt
     5161 atgcacacct ttcctttttt cgtcaacggt gtccagaatc attttattta cctcgggtac
     5221 ttatgctgat ttttattatt atggggaagg tgttatttat aagtttcatt tatgccgt
//
LOCUS       DAZPFP010000216         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPFP010000216 DAZPFP010000000
VERSION     DAZPFP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.219"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHUR4_003656"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHUR4_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUR4_003657"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUR4_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1457051.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUR4_003658"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUR4_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1457052.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUR4_003659"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUR4_003659"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1457053.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHUR4_003660"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHUR4_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHUR4_003661"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHUR4_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHUR4_003662"
     CDS             complement(3604..4419)
                     /locus_tag="ACHUR4_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1457054.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHUR4_003663"
     CDS             4516..>5150
                     /locus_tag="ACHUR4_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1457055.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1225 g         1376 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZPFP010000217         5108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPFP010000217 DAZPFP010000000
VERSION     DAZPFP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.220"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUR4_003664"
     CDS             <1..244
                     /locus_tag="ACHUR4_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457056.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHUR4_003665"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHUR4_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUR4_003666"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUR4_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1457057.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUR4_003667"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUR4_003667"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1457058.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUR4_003668"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUR4_003668"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1457059.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUR4_003669"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUR4_003669"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1457060.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1166 a         1298 c         1465 g         1179 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataa
//
LOCUS       DAZPFP010000218         5093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPFP010000218 DAZPFP010000000
VERSION     DAZPFP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.221"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            23..853
                     /gene="rcnA"
                     /locus_tag="ACHUR4_003670"
                     /pseudo
     CDS             23..853
                     /gene="rcnA"
                     /locus_tag="ACHUR4_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHUR4_003671"
     CDS             1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHUR4_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1457061.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1492..2525)
                     /locus_tag="ACHUR4_003672"
                     /pseudo
     CDS             complement(1492..2525)
                     /locus_tag="ACHUR4_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2541..5003)
                     /locus_tag="ACHUR4_003673"
     CDS             complement(2541..5003)
                     /locus_tag="ACHUR4_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1457062.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            complement(5019..>5093)
                     /locus_tag="ACHUR4_003674"
     CDS             complement(5019..>5093)
                     /locus_tag="ACHUR4_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457063.1"
                     /translation="KSNNANNWYLTIINDHGNYISDKI"
BASE COUNT         1357 a         1235 c         1034 g         1467 t
ORIGIN      
        1 attaatacta agaattatta tcatgaccga atttacaact cttcttcagc aaggaaacgc
       61 ctggttcttc atccccagcg ccatcttact tggtgcgctt catggcctgg aaccagggca
      121 ctcaaaaacg atgatggcgg cgtttatcat cgccatcaaa ggcaccatta aacaggcggt
      181 gatgctcgga ctggcagcaa ccatttcgca taccgcggtg gtctggttga ttgcctttgg
      241 cgggatggtg atcagtaagc gctttactgc tcaatcagca gaaccgtggc tccagctgat
      301 ttccgcagtg atcattattg gcaccgcgtt ctggatgttc tggcgtacct agcgcggcga
      361 acgcaactgg ctggagaata tgcacgggca tgattatgag catcatcacc atcatcacga
      421 tcacgaacat caccacgacc aaggacatca tcaccatcac gaacatggcg agtatcagga
      481 tgcccatgca cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcat
      541 caactggcaa attttgttat ttggcttaac cggtggcttt atcccctgcc cggcagcaat
      601 taccgtgctg ttgatatgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
      661 cagtttcagc attggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag
      721 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
      781 tttttccagt ctgttgattg gcttagtcgg tgtgtatatg ggcgtacatg gcttcatggg
      841 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
      901 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
      961 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
     1021 tgacaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
     1081 aaaaataaga tgttgctggg tgtgcttttg ctggttacca gtgccgcctg ggccgcacca
     1141 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag tagtttcatt
     1201 gctgacttta agcatttcaa accaggtgat accgtcccag agatgtaccg caccgatgag
     1261 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
     1321 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
     1381 tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg
     1441 cagacacctt gttatttttt attattagcc acttgctcgt tttgcttgtt attagtcgta
     1501 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
     1561 gacgtagtaa ctggctgtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
     1621 cgcaatttct tttaaatttg tgtatccttt actgctgtta gtgatattta aaagtgtgac
     1681 tggcgaacca ttactttcaa gaataaatcc tacgcattat cttccgcacc tttcagtatt
     1741 gtgttgctgt tggttaacaa gtcagaactt gataaataca cctgcatttt ccgtgatgtt
     1801 tgattttgtt gacctaataa atttttacaa cgcagaataa tatccctttt tttactattt
     1861 attgttgctt tgttacctgt ggctggtaat tgtaaaacgg gaatactgtc taaagtaatt
     1921 aataaatcgt cctgtttgca ggttgtggtt atcgggttgt atgacaatgt cagattgaag
     1981 gaatagacac ctgcattttg tgccaatgca tttctgtagc tatttacaca tgcaccccat
     2041 gacttgccac ctaataaaca cagtagaaat tgtactgcgg cactcccgcg tgtcgatgat
     2101 gaagcactgg ataatgtcgc aacaccatta atcatcactg aaccaccgcc ccccgcagaa
     2161 atattcactt ttgcattact ggccgcaggt gtcaccgtta ccgaataacc actggtgaaa
     2221 ctgctctgat tatttgacaa cgtgatgttg tttttattgg tccattcaaa atgtaaattt
     2281 aatttaatgg tatcgttata aagtccaaca ataatatccg atgggctcat aggggtgata
     2341 gtatttgtac tcacgcatga cgaatcatta aaattatgct catatgtcgc tatttgtgtt
     2401 tgagtaagtg caaaattccc ctgaacaacc aaattgtgct gaacagataa ctcactataa
     2461 catgccgcat agctcacagg cataaccatc atcattaata taaatagcat tatgcgaatt
     2521 tccatagaac cacctgacta ttactggcaa atataatttc tactttcatc aatctcttta
     2581 ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa ttgctacatt aaccgatggc
     2641 ggtatttcat tggtgcgaat aaataactgg cttccctggc cgacaacgcc aatattatga
     2701 ccatggatat cattgacttc ataaccaaac gttaatggtt gaccatccgc tcttaacgct
     2761 tttataaacc agggcttgcg ctgatcggta tcaaaattaa ccagtacaac cgcgccgcga
     2821 taaggggcgg caattttccg gttgccacgt aattctgctt cgctatcgct ttgcgacaca
     2881 tccagcatca ggtgattttc ccgataaggt gtcattccgt cgtataccac cactccatta
     2941 cggtttgttg tgcgatattt ttgcccattg acataagcat ctttaattcc tggcgcattc
     3001 atcacagcaa acgtttcgga aagacggttc gccagattaa cgccacccga ccaggcgaca
     3061 atgccccctg aaacactggc tccagtctgt cgataagtac tcgactgact ataactgcca
     3121 ttcactgtcg caaccggcgc gttccaggtc aaattcgccc cagctgtcgt ttcatttccc
     3181 tgatgttgat gactcaggtt gacaccataa ttgaactgat cccgactccc tactgttcct
     3241 gataatcccg tattatttga ggcaaaaccc tgatcatcaa acgtcgttga gttagacata
     3301 tatatttgcc gacgaggcgt cgtaacgtca tcaccccaat cacagggaat tgatataaaa
     3361 atattaaaac gtttctcttc atgatgattc tcgccatacg cctggcttgc cgcgagggta
     3421 tagcttatcc gccgccagtt attggaataa ctcaactgat aatccttact gctgccgcta
     3481 cgcccccagt aatctcgcca taacgtactt aaggacacag aaccccagcc ttctggcaat
     3541 gactggctca tattggcaga aaagctattt ttacggccaa aatcgttctg gtaataatcg
     3601 gcaatgtcat agatatcgtt ttcatcacgg cgataattat ctttattgtt tgcccaaacg
     3661 tgatcgttaa atgtccggta atcacgcgac gaataacgcc aggccgccag accaaaacgc
     3721 gtcgacgttt ggctcacaaa tttgttgtag gcaatttgat aactttgccc gtcaaacaca
     3781 tcgccgttgt cttgtttact atgcgacttc gtggcatcga cggaaattgc gccaatgcgc
     3841 gtgttccaac ccgttccgag ggtaaacgca tagtaattat tagcaaccat cgtgccgcca
     3901 tacagcgtca ataagttatt aaaaccatac tgataacctg cctggacaaa atcactttgc
     3961 ttgctcgccc ccgccgcaaa atcatatttc gacacgccgg gttgcagcat atttggcacc
     4021 gctgcataag gcaccagata ggtggttacc gagccgtccg cctctttcac gctaacatca
     4081 agatctgctc caccaccggc caactgcaaa tctgtaatcg caaacgggcc aggaggaacc
     4141 tctttctgat aaaccacaaa accattctgt tcaatagtta ccagcgcgtt actctgagca
     4201 atcccctgca cccgtggcgt aaaattttgt ttcgagttag gcaacatctg catatcacga
     4261 aacaaccgca caccgctgaa gcgaacagaa tcaaaaatat cgcttgatgt gtacatatca
     4321 cccacgcgaa gcgtgccgag aaattgggca aatccacgtt ccagatacag ggtattgctt
     4381 ttccacaccc ctggattgtt atttgtttta ctgaaactgg catcagaatg cagttgccac
     4441 tccagtaaat ttaacccgct gttaaaacgt acatatgtac tcttgttgtt acccgacgct
     4501 ttatagtcgc tgtaatactg actcacataa taagaagtat aaaacgcatt aataccccgc
     4561 tcccagtttt ccggtggaac atagccactt tccagttctt ccacccaagc ctgcgggaca
     4621 ctgaaatcga gacgaaaaac cccgatatcc cagctatagc tcccaccctg aacaagttgc
     4681 tcaaatgtta aacattgctt accgctggcg aagttatcgc tattaatgcc taaccgcttg
     4741 ataacttctc ttgataaaca tgtttcttgc gggttgtctt taacaataat ctcatatttc
     4801 ccgcgccatt gcttattgac ataaatatcg atgtcatact gccccggtaa gggttgatta
     4861 tcatcaagac gaatatttgc aacctgctgg tctttcattc cacctatcat aaaatgggta
     4921 tcaaaggttt cttcagctgc ataagcttca atgccgagca ataacgctac gattgctgat
     4981 gcaagtgggg tcattctcaa cataggcagc tcctgcaatt aaattttgtc actaatatag
     5041 ttgccgtggt cattgataat ggtcagatac cagttatttg cattattact ttt
//
LOCUS       DAZPFP010000219         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPFP010000219 DAZPFP010000000
VERSION     DAZPFP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.222"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUR4_003675"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUR4_003675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR1457064.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHUR4_003676"
     CDS             1020..1703
                     /locus_tag="ACHUR4_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR1457065.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHUR4_003677"
     CDS             1700..2161
                     /locus_tag="ACHUR4_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR1457066.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUR4_003678"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUR4_003678"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1457067.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUR4_003679"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUR4_003679"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR1457068.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUR4_003680"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUR4_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR1457069.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUR4_003681"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUR4_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR1457070.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHUR4_003682"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHUR4_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457071.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZPFP010000220         4934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPFP010000220 DAZPFP010000000
VERSION     DAZPFP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.223"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            302..1687
                     /gene="dcuC"
                     /locus_tag="ACHUR4_003683"
     CDS             302..1687
                     /gene="dcuC"
                     /locus_tag="ACHUR4_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1457072.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1728..2069)
                     /locus_tag="ACHUR4_003684"
                     /pseudo
     CDS             complement(1728..2069)
                     /locus_tag="ACHUR4_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2407..2916)
                     /locus_tag="ACHUR4_003685"
     CDS             complement(2407..2916)
                     /locus_tag="ACHUR4_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1457073.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            3088..3324
                     /locus_tag="ACHUR4_003686"
     CDS             3088..3324
                     /locus_tag="ACHUR4_003686"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457074.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            3299..4930
                     /locus_tag="ACHUR4_003687"
                     /pseudo
     CDS             3299..4930
                     /locus_tag="ACHUR4_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1230 a         1095 c         1225 g         1384 t
ORIGIN      
        1 atatatatcg tcaacccatg ctcatattag attatttttt gagcgcacag ccgcagctaa
       61 caccattgca attaacaaat ttgcatcaat ccaccatcaa tttacacaca ttattatgtg
      121 ataattgcca accgctaaat atgcgttttg ttatctatgt ataaaaacag caacttcaat
      181 gtcttaatgg cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat
      241 tttttcctta agctttagga agtttttatt atttactttg ggcctggaga caggaaaaat
      301 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tggctcgcta
      361 catcattaaa gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat
      421 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
      481 cgccacggat atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg
      541 catgatgatt atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga
      601 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
      661 gattgccgcc tattttgttg cttgtctgat gtcactggcc gtctcttccg caaccggtct
      721 gggggttttg ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagtcgtgg
      781 cgcagcagct gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
      841 tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
      901 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
      961 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
     1021 cactgccccc gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt
     1081 tgacggtaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
     1141 tgcctccatt ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga
     1201 agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
     1261 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
     1321 tacctcgttt ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct
     1381 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
     1441 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actattccga tgctgcaggc
     1501 gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat
     1561 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
     1621 agtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg
     1681 caagtaaata gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
     1741 tatatgcgtt gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
     1801 ctggcgcaat attcaagata acgcctggca gtggtgcggc tgatggtcag tgcctgcgcc
     1861 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
     1921 gtcaaggggt caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta
     1981 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
     2041 tggcggaaac gggttagcgt ttgccccagt gtattgattc acttgaagta cgaaaaaacc
     2101 gggagaacat tggattattc gggatctgat gggattagat ttggtggggc ttgcaagcct
     2161 gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag atagtaaaaa attgcttttg
     2221 tttcattgaa aaaacgaaaa acaaaaacac tataaatcat ttcaatcata gcttcaaaaa
     2281 acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac tcatatcaca aatcagctca
     2341 tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt agctggctca
     2401 tggttgttat acggcttttt tcgcatcttg tggttccaac aaaggagcta caggctcttc
     2461 ctgtttatga acaccataca ttgacaaaat ctcttggcga gtttctggag gtaatttatg
     2521 cagcgccaaa gctgtttgta gtgtttggcg atcaataccc agcgttgcca atagggtgtc
     2581 aacggagatg gaggggcgtt cttgcattaa tccggaaagc ccatatttag ccatgtttag
     2641 ctctatctct cggacttcat tttctcctag tttattgatt aacagggaga acattttcaa
     2701 tgtatcagca ttcatttctt cgatagcaat tttttgcata gccggtattg ccgggatata
     2761 ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc ggccatcctt ctttctttgc
     2821 tcgtttatgc accccctgta ttgtcccagg cattccatcc agttcagtaa tttgtttggg
     2881 tgtcatccac tggtttatct catttgcgct tttcataaac taagtttacc agatttatct
     2941 atataaaaat caatggatta caaaataaac cacattaact gatataaacc ggtttggttt
     3001 atttggtaat gtataaaaca gataatcaag acgaattatt tgttttggta accctttatc
     3061 atacgcaata tggttggtgt gcgaaagatg agtaacgatt ggcatcgggc cgacgtgatt
     3121 gcggccctca aaaaaacagg aacccctctg gcaaaattgt ccagacgttc aggttattca
     3181 accgggacgc ttgcaaatgc attagtcagg ccatggccta aaggtgaacg cattattgct
     3241 gacgccttaa acatgaaacc tgaggaaatt tggcctagta gatatctaag ggatcacaat
     3301 gagagagtgg atatcagcaa ttgaatgcat gggtttatct ggtttacctg gttcattaat
     3361 gggggttcac aaaagagctc agagagaagg atggctcaag agaagaagag aaggaattag
     3421 aggggttgct tacgaatacc atatatcttc tcttcctaac attgctcgag ctgaagtttt
     3481 gctccaacaa ggaaaattta aaaccagtca gggatgtttt gaaatcgccc gccccacgct
     3541 ggaagctcat gattatgatc gtgaggcact atggagcaaa tgggataaag ccagcgattc
     3601 ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag gccacagacg aaatgctgaa
     3661 ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca gggcattacc aggtcagcgc
     3721 atccactttg cgggacaagt attaccaggt acagaagttt gcgaagcctg actgggcggc
     3781 tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt cacaaaagtg aatttgacga
     3841 ggatgcctgg cagtttctga ttgcagatta tctgcgaccg gaaaaacccg ccttccgcaa
     3901 atgttatgag cgtctggaac tggcagcccg cgagcatggc tggagtattc cctcccgtgc
     3961 cacggccttt cgccggattc agcaactgga cgaggcaatg gttgttgcct gtcgtgaagg
     4021 tgaacatgca ctgatgcatc tgataccggc acagcagcga actgtggaac acctggacgc
     4081 catgcagtgg atcaacggcg acggttatct gcataacgtc tttgtacgct ggtttaacgg
     4141 tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg aaaacccgaa aaattctggg
     4201 ctggcgctgc gatgtgagcg agaacattga ttcaattcgc ctctcgttca tggatgttgt
     4261 gactcgctac agtatcccgg aggattttca catcaccatt gataacaccc gtggtgctgc
     4321 gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc tttaaggtaa aagaggacga
     4381 tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac tggacaagcg ttgttgccgg
     4441 taaaggctgg ggccaggcaa aacctgttga acgtgctttc ggtgttggtg ggcttgagga
     4501 atacgttgat aagcatccgg cactggctgg cgcatatacg gggccaaatc cgcaggcaaa
     4561 acctgataac tatggcgacc acgctgttga tgcagagctg tttctgaaaa cctttgccga
     4621 aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca gaaatgtgcg ggggcaaact
     4681 ctcgtttgat gatgttttcg agcgtgaata cgccagaacg attgtgcgta agccaaccga
     4741 agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg aacgtttcac gcaaaggcga
     4801 gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa aacgtttatt acaacatggc
     4861 attaatgaat gccggagtga aaaaagttgt ggtcagattt gatccacagc agttacacag
     4921 cacggtttat tgag
//
LOCUS       DAZPFP010000221         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPFP010000221 DAZPFP010000000
VERSION     DAZPFP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.224"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUR4_003688"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUR4_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1457075.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHUR4_003689"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHUR4_003689"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR1457076.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUR4_003690"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUR4_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUR4_003691"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUR4_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1457077.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHUR4_003692"
     CDS             4169..4342
                     /locus_tag="ACHUR4_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457078.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUR4_003693"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUR4_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1457079.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUR4_003694"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUR4_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1457080.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHUR4_003695"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHUR4_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZPFP010000222         4758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPFP010000222 DAZPFP010000000
VERSION     DAZPFP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.225"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUR4_003696"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUR4_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR1457081.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHUR4_003697"
     CDS             2739..4208
                     /locus_tag="ACHUR4_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1457082.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4758
                     /locus_tag="ACHUR4_003698"
     CDS             4208..>4758
                     /locus_tag="ACHUR4_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1457083.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGA"
BASE COUNT         1375 a         1157 c         1148 g         1078 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctga
//
LOCUS       DAZPFP010000223         4737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPFP010000223 DAZPFP010000000
VERSION     DAZPFP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.226"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(30..1040)
                     /gene="ascG"
                     /locus_tag="ACHUR4_003699"
     CDS             complement(30..1040)
                     /gene="ascG"
                     /locus_tag="ACHUR4_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1457084.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(1102..1173)
                     /locus_tag="ACHUR4_003700"
                     /pseudo
     CDS             complement(1102..1173)
                     /locus_tag="ACHUR4_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1183..2892)
                     /gene="hycE"
                     /locus_tag="ACHUR4_003701"
     CDS             complement(1183..2892)
                     /gene="hycE"
                     /locus_tag="ACHUR4_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1457085.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(2910..3833)
                     /gene="hycD"
                     /locus_tag="ACHUR4_003702"
     CDS             complement(2910..3833)
                     /gene="hycD"
                     /locus_tag="ACHUR4_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1457086.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(3836..>4737)
                     /locus_tag="ACHUR4_003703"
     CDS             complement(3836..>4737)
                     /locus_tag="ACHUR4_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR1457087.1"
                     /translation="IALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHR
                     DIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARL
                     LGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICC
                     VIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLP
                     SRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSA
                     LLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         1120 a         1395 c         1258 g          964 t
ORIGIN      
        1 ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa gggcaatgag tgagtcgcgg
       61 cggatcagtt ttccgctaaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa
      121 atcagccgtc caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc
      181 gccggaacga tatagggggc aattgcgata tcatcgaatc cgataactga cacctgctct
      241 ggcaccgcta cgccgcgctc gtgcaacgct ttgatcgcac ctatcgccat atcgtcgtta
      301 ctggcaacta acgcgctaaa tttagcccct cgttcgagca acgtttctac cccttcggcc
      361 ccgctggcgg gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc
      421 tgcgacagcg cgtctttata cccggcaaga cgttcaatgc tggtggggga atccatcgag
      481 ccggtaagga aagcaatctc ctgatgcccg gcgtttatca actctgccac ggcgttaaag
      541 ctggtctgtt tatgatcgca ccagacgcta tggctgctat tattgcgcag gcggcgatta
      601 agcaccatta tcggctgact gtgcgcgtca atgatgtcat caatctcatc cacgcttaaa
      661 aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg
      721 cgctcttctt cggcgctgtg tttaccatct gccaatagca actgtcgccc tttctcttcc
      781 gccattcgcg cggcatgaaa cagtaattca ctaaaataaa tgccgtgata aagcgtgttg
      841 gtcactacca gccccagcgt ctgagtactc ttcgccgaca gattgcgggc cagcaaattt
      901 gggcggtaac tgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg
      961 tagccattac ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc
     1021 acttccagca tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc
     1081 accgtttgcc gattgtcctt gcgcaatcgg ctccagcgga taagacgagg tcgccgtgcc
     1141 ggttttgatg acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt
     1201 tttacgctca atgctgtaac gctcgagttc tttgtacggc accactttgc tcttcttctt
     1261 acgcacatcg actacggtca tgcggtcggt acaggagtag caagggtcca ggctaccgat
     1321 aatcagcggc gcataggaaa cggtattgcc acgcagcatg tagcgcaggg tcggccagtt
     1381 cgcgtaggtc gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct
     1441 ccagtggata tcgtcgccgc gcggcgcttc ggcaaagccc agcgcgaaac ggtgcggaat
     1501 gtaggtaaag ccttccacca tcagcggacc gcccggcagg ttatccagac cgtagtcgat
     1561 catattcagc gcggtataga cctcgttgat tcgcactttc agacgggaaa taacgtcgca
     1621 gccctgctcg ctgtggactt ccattggcag caggccataa ccgacaaacg ggtgatcggc
     1681 gcgagtatcg cgggcgagac cgctggcgcg aaccatcggg ccaacgttac tgaaatcgcg
     1741 ggcgatttcc gggtccagac gaccaatgcc gacggtgcgt tgttccatgt tcggcgtact
     1801 cagcagcaca tccaccagct cctgcacttc acgacgcatt tgttgcgcca gctggcgggt
     1861 ctggatcatg tcatctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta
     1921 ggttttacgc gcaccggtaa ggatctctgc cattttcatg gaggtttcac gcacgcggaa
     1981 gaactgcata aagccggagt cgaagccggt aaagtgacag gcgaggccga ggttgagcag
     2041 atgcgggtgc aggcgttcca cctccagcag aatggcgcgg atcatctgcg cgcgttctgg
     2101 cacctgaata cccatcgcgt tttccaccga cgtggtgtag gcggtgctgt gggcaaagcc
     2161 gcagatcccg cacacacggt cagagaggaa ggtcacttcg ttataaccca tgcgggtttc
     2221 cgccagtttt tccataccgc gatggacgta gaacagacgg tagtcggcgt cgataatgtt
     2281 ttcgccatcg acgaacagac ggaagtgacc cggttcgtca gaagtgacgt gcagcggacc
     2341 aatcggcacg acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc
     2401 atcggtagtc ggtgccggac gctgacgata atccatgctg tctttacgca gcggataaag
     2461 ttcatccggc cagtcatccg gcagcaccag acgacgttca tccggcagac caaccggaat
     2521 caaaccgtac atatcgcgca cttcgcgctc gccccacacc gccgccggaa cgcgcggcgt
     2581 cacggacgga tactccggtt tgttggcgtc aacttcgacg cgaaccgtta cccaacactt
     2641 ggtgcccttc tccatcgaca gcacgtaata aacggcgtaa tgaccattca gtttgcgttc
     2701 gtcgttacca aacagcaccg acagccagcc accctgcttg tagtagagaa actccaccac
     2761 ttccggcaga tagttcacct ttacggtgac agtcagctga tctttggtct gccaggcgtg
     2821 gtccagcacg acgcctagaa atgcctcatt cagcgcggcg agataatgtt gacctaattt
     2881 ttcttcagac atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa
     2941 aatgcaaagc caaacccagc ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc
     3001 atgctgtttt cgaacagcgc gataaccagg acgccgacca ccagtttgac gatggcaatc
     3061 accagcgcca gcagcagtcc accgacggtg aaggtttcca tttgtcccca cggaataaac
     3121 accccgacga acatctgcaa caccaccagc tgtttcaggc tgatacccca tttcatgacg
     3181 ccaaagccgc taccgctgta ttcggagagc ggaccttcct gcaactcctg ctcggcttcc
     3241 gccaggtcga acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcgcagagc
     3301 gccagcacca gcgggatgct ttggctcagc ggccagtgat aaacggtgtc ggtaatgttg
     3361 ctgatgttgg tggaaccggc aacctgtgcg gcaacccaca gaccaagcag caacatcggt
     3421 tcgaccagca cgccaagcat cgcttcacgg ctcgcaccga tagcggtaaa cgggctaccg
     3481 gtatccagac cggaaatggc aaagaagaaa cgcgcgatgg cgaagagata cagtaaggtg
     3541 atcaaatcgc ccagttgcgg cagcggagaa ccgacggtca ccaccggcag cgcagtagcg
     3601 atagtcagca tgacgcccac catcacatac ggcgtcaggc ggaacatcca gccagaggca
     3661 tccgggccaa cgctctggcg gcccagcagt ttgataatgt cgcgatactc ctgcaacacg
     3721 cccggcccgc gacggttatg cagacgggcg cgcgccacgc gggtaatacc ggagagcagc
     3781 ggcgcaacgg caaataacac cagcgcctga attaacggat ataaaacact cattctcagg
     3841 ctcctcgtga aacaataatc accaccagta ccgccagttc aaccagcgcc atccggcgga
     3901 acagcaacgc actaccttcg cactgccagc ccggcactag cgacaccgga ttcagccatt
     3961 tgcgcagttt cagcactggc gcaaacgcct gtttaaccgg catggcaaaa ccgtgagcgg
     4021 taatcaccat tgatttttca tggtcgtagc cgcacaccca ggccgcaccg cgggaacgtg
     4081 acggcaggcg gtcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa
     4141 tcagcagcaa tgtgatcatt ggttgagaaa cggtggtgtt agcaggctcc agaggcagag
     4201 gtacagcagc agagagcatc ggcagcagcc acggcgcagc aacaccgcca attacgcagc
     4261 aaatcgccag tgccactacg cttacgctca tcaggagcgg cgcacaggtg gcgttttcgg
     4321 cttctttggt acgcggcgcg ccgaggaacg tgacgccata gactttcgcc atacacatca
     4381 ccgccagcgc accggtaatt gccagaccca cagcgagcag cggccccagc agacgggcaa
     4441 caaacgcgcc gctattgctc agtttgaaga aggattgata gataacccat tccccggcaa
     4501 acccgttcag tggcggcagc gcagccattg ccatcaaccc gactaacatg gcgatggaga
     4561 taaccggcat tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga
     4621 accagacgct ccccgccccg aggaacagta cgcttttgaa caggctatgg ttaagcagat
     4681 ggtacagacc accgaccagg ccaagagcaa tcagcgccgg ttgttcgagc gcgatac
//
LOCUS       DAZPFP010000224         4709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPFP010000224 DAZPFP010000000
VERSION     DAZPFP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.227"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            18..746
                     /gene="gadW"
                     /locus_tag="ACHUR4_003704"
     CDS             18..746
                     /gene="gadW"
                     /locus_tag="ACHUR4_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1457088.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1109..4222)
                     /gene="mdtF"
                     /locus_tag="ACHUR4_003705"
                     /pseudo
     CDS             complement(1109..4222)
                     /gene="mdtF"
                     /locus_tag="ACHUR4_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(4247..4504)
                     /locus_tag="ACHUR4_003706"
                     /pseudo
     CDS             complement(4247..4504)
                     /locus_tag="ACHUR4_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1197 a         1172 c         1164 g         1176 t
ORIGIN      
        1 aaatataagg gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg
       61 atatttatca tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga
      121 aaaatttggc agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg
      181 agctgacagt ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac
      241 atagcaaaga ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt
      301 ttgatagctt caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt
      361 cctgtttatc gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca
      421 gcactgtttc aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc
      481 tgcgcgatat cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg
      541 atgaaaatac ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat
      601 tactcgagtt acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta
      661 cgtcgtactt tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc
      721 aacattcgcc aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca
      781 cacctgcttc atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct
      841 ttcgcagttt gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga
      901 ttattaagct gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat
      961 tgatgacgaa tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc
     1021 tgacggttag ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt
     1081 cacgttcaac cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt
     1141 ccactacaac gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc
     1201 cgcccatcac gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa
     1261 ccagcggcag cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca
     1321 tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa
     1381 cggcaaattc gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac
     1441 caacctggaa gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa
     1501 cgacgcctaa cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg
     1561 cggcgagggc gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg
     1621 aggataacgc ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt
     1681 tagcgaccag gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt
     1741 cacctaaaat ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg
     1801 tccattcggt agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat
     1861 accattggtt gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt
     1921 ttttcacccg cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa
     1981 ttgtctggtt gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt
     2041 tcactttgaa catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag
     2101 gcgattgcgc tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca
     2161 ggttaccgtt gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg
     2221 cgggtaagtt gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc
     2281 gctgaatgat cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac
     2341 tgataaacgc caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa
     2401 acaccgactg aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta
     2461 ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc
     2521 cctgatcctc ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg
     2581 cgcaaatcag caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc
     2641 tatcggtata gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt
     2701 taggtttgtg accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca
     2761 ggctcattgc cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc
     2821 ggtagatctc cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa
     2881 caacggcaat cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct
     2941 tcggcggtag cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt
     3001 catccaccag taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga
     3061 aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg
     3121 ggatgatggt ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga
     3181 tagcctcaac cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt
     3241 cgtaaggata aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct
     3301 ctttgactgc ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc
     3361 cggcaacagg tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga
     3421 cgcgggcgac atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt
     3481 tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct
     3541 ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct
     3601 taatctggga aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca
     3661 gccagatacg catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac
     3721 ggcttagcgg gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc
     3781 tgccgttatc agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc
     3841 taatcccctg ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt
     3901 gcacctgaac ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg
     3961 cattacccgc cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat
     4021 tttgctcaat cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg
     4081 tagcgctgac ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca
     4141 tgatcgccag accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc
     4201 gatcaataaa atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt
     4261 cggtgctggc gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc
     4321 gttgcaaacc ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact
     4381 ggtcgccgat ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca
     4441 gaatgagcgc cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta
     4501 atataaagcg cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc
     4561 gtgtgatagc aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta
     4621 tatattttta ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc
     4681 agcggtaaat cgaagtaaag ggagtgaat
//
LOCUS       DAZPFP010000225         4611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPFP010000225 DAZPFP010000000
VERSION     DAZPFP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.228"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..121
                     /gene="yecU"
                     /locus_tag="ACHUR4_003707"
     CDS             <1..121
                     /gene="yecU"
                     /locus_tag="ACHUR4_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1457089.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTTNFHCNMTRKINEYA"
     gene            108..332
                     /gene="yecF"
                     /locus_tag="ACHUR4_003708"
     CDS             108..332
                     /gene="yecF"
                     /locus_tag="ACHUR4_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1457090.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(365..985)
                     /gene="sdiA"
                     /locus_tag="ACHUR4_003709"
                     /pseudo
     CDS             complement(365..985)
                     /gene="sdiA"
                     /locus_tag="ACHUR4_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(1215..1967)
                     /gene="tcyN"
                     /locus_tag="ACHUR4_003710"
     CDS             complement(1215..1967)
                     /gene="tcyN"
                     /locus_tag="ACHUR4_003710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1457091.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(1964..2632)
                     /gene="tcyL"
                     /locus_tag="ACHUR4_003711"
     CDS             complement(1964..2632)
                     /gene="tcyL"
                     /locus_tag="ACHUR4_003711"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1457092.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2647..3633)
                     /gene="dcyD"
                     /locus_tag="ACHUR4_003712"
     CDS             complement(2647..3633)
                     /gene="dcyD"
                     /locus_tag="ACHUR4_003712"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1457093.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3738..4538)
                     /gene="tcyJ"
                     /locus_tag="ACHUR4_003713"
     CDS             complement(3738..4538)
                     /gene="tcyJ"
                     /locus_tag="ACHUR4_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1457094.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT         1152 a         1251 c         1070 g         1138 t
ORIGIN      
        1 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
       61 aacatttttc accacaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
      121 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
      181 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
      241 tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
      301 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag cgtgcctttc
      361 agccggttat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc gccgtccatt
      421 tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact atttcatcat
      481 ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt aattgcagtt
      541 catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa aagcccagcg
      601 cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt gcgtgcgcgg
      661 cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa tggccctgac
      721 taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc tgataataac
      781 taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg aatggtaccg
      841 ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga agctctattt
      901 catggtaggc ctcttctgcg gcctccatcc tctgaaaacg caacagcatc gtgcgacgcc
      961 agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt gagtatcatt
     1021 tataatgata attcaaacta tcacataaat aagatttata tataatttat attattcagg
     1081 caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt tatgctgatt
     1141 tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga atcgtcgggg
     1201 ctgatttttt cttattattg cagcagaagc ttctcgagga actggcgggt gcgaggctgc
     1261 tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc ctggtccata
     1321 aagatcgccc ggtccgcaac atcccgggca aagctcattt cgtgtgtcac aatcaccatc
     1381 gtgcgctttt cctgcgccag ctggcggatg gtgttcagga cttcgcccac cagctctgga
     1441 tccagcgccg acgttggctc gtcaaacaaa atcacctcag gacgcattgc cagtgcacgc
     1501 gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct ggtttcttta
     1561 ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc ttctttcggt
     1621 tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg atgcggaaac
     1681 aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat cagcgatttt
     1741 tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat cgttcccgct
     1801 tccggttgtt ccagcagatt tatgctacgt agcaatgtgg ttttgccgga accactcgga
     1861 ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg cagcaccgtc
     1921 tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttt ggttctctct
     1981 cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca gtcgccatga
     2041 tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg cgcgaggtaa
     2101 tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc gacgtatctt
     2161 ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc gcctgtggca
     2221 aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg gcttcccact
     2281 gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcggcatag gcggcagtgt
     2341 tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca aactgcggca
     2401 ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa atagagatat
     2461 aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc gcgagaataa
     2521 acccgagtag taagccaaaa aacatgccgc cgatactgag ttgcagggta tacccagccc
     2581 ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc atgagcgttc
     2641 ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg tatgaataaa
     2701 cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca gccccgccat
     2761 cgcttttccg gtatacacag gatcaagcag aatgccttcc agacgcgcca gcaatttcac
     2821 tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt catcccagag
     2881 taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt gtaggttaac
     2941 cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca gttcgctttc
     3001 aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac tgcccgatgc
     3061 gactaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt ccagcgcact
     3121 ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga catacgggcg
     3181 aaagccttgt gcttcgactc gcgtcgccag ttcttccagt tgggcattgg gatcggtcag
     3241 tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat tgccgttggt
     3301 taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc agtgcagacc
     3361 gagtttcgcc gcgactgcgg cagtctggcg cacatggtta gactggatcg cgccggcagt
     3421 aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca gcttacgtaa
     3481 tttattgccg cccattgcca tgggggtgac ctcatcccgt ttgatgaaaa tttcccgtcc
     3541 aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga taaactccaa
     3601 ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt gttgttgtga
     3661 ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga aagcgccctt
     3721 ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc ggaaagagct
     3781 tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt cagcaggtct
     3841 tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc accggttact
     3901 gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc aacgaggatc
     3961 gcatcgatac gccctacgcg cagatcctga tatttggtcg ggtcatcatc ataggtacgc
     4021 acatcgacac cctgaacatt ctgccgcagc cactcttcat agttggtgcc cagaccgaca
     4081 cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt accttttttc
     4141 accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt tttcttgcgc
     4201 tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc cagcgacgcc
     4261 agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg ttttgccagc
     4321 tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg aaaactgaac
     4381 ggcggataag tgccttccag ccctaccagc agcgtgccgc tctctttgac tttattaagc
     4441 agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac ggccatcaca
     4501 cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa tgttgttatg
     4561 tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct a
//
LOCUS       DAZPFP010000226         4602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPFP010000226 DAZPFP010000000
VERSION     DAZPFP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.229"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUR4_003714"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUR4_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1457095.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUR4_003715"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUR4_003715"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1457096.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUR4_003716"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUR4_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1457097.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUR4_003717"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUR4_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1457098.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZPFP010000227         4536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPFP010000227 DAZPFP010000000
VERSION     DAZPFP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.230"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            251..1108
                     /gene="focA"
                     /locus_tag="ACHUR4_003718"
     CDS             251..1108
                     /gene="focA"
                     /locus_tag="ACHUR4_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1457099.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1163..3445
                     /gene="pflB"
                     /locus_tag="ACHUR4_003719"
     CDS             1163..3445
                     /gene="pflB"
                     /locus_tag="ACHUR4_003719"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1457100.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3637..4377
                     /gene="pflA"
                     /locus_tag="ACHUR4_003720"
     CDS             3637..4377
                     /gene="pflA"
                     /locus_tag="ACHUR4_003720"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1457101.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4459..>4536)
                     /locus_tag="ACHUR4_003721"
     CDS             complement(4459..>4536)
                     /locus_tag="ACHUR4_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457102.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1154 a         1115 c         1094 g         1173 t
ORIGIN      
        1 tattgttact ttattcgcct gatgctccct tttaattaac tgttttagcg gaggatgcgg
       61 aaaaaattca actcatttgt taatttttaa aatttatttt tatttggata atcaaatagt
      121 tactccgtat ttgcataaaa accatgcgag ttacgggcct ataagccagg cgagatatga
      181 tctatatcaa tttctcatct ataatgcttt gttagtatct cgtcgccgac ttaataaaga
      241 gagagttagt gtgaaagctg acaacccttt tgatctttta cttcctgctg caatggccaa
      301 agtggccgaa gaggcgggtg tctataaagc aacgaaacat ccgcttaaga ctttctatct
      361 ggcgattacc gccggtgttt tcatctcaat cgcattcgtc ttctatatca cagcaaccac
      421 tggcacaggc acaatgccct tcggcatggc aaaactggtt ggcggcattt gcttctctct
      481 ggggctgatt ctttgtgttg tctgcggagc cgatctcttt acttccacag tgttgattgt
      541 tgttgctaag gcgagtgggc gcatcacctg gtgtcagttg gctaaaaact ggctaaatgt
      601 ctattttggc aacctggtcg gcgcactgct gtttgtactt ttaatgtggc tttccggcga
      661 gtatatgacc gcaaatggtc aatggggact aaacgtccta caaaccgccg accacaaagt
      721 gcaccatact tttattgagg ccgtctgtct tggtatcctg gcaaacctga tggtatgtct
      781 ggcagtatgg atgagttatt ctggccgcag cctgatggac aaagcgttca ttatggtgct
      841 gccggtcgcg atgtttgttg ccagcggttt tgagcacagt atcgcaaaca tgtttatgat
      901 cccgatgggt attgtaatcc gcgacttcgc atctccggaa ttctggaccg ctgtcggttc
      961 tgcaccggaa aatttttctc acctgaccgt gatgaacttc atcactgata acctgatccc
     1021 ggttacgatc ggtaatatta tcggcggtgg tttgttggtt gggttgacat actgggtcat
     1081 ttacctgcgt gaaaacgatc accattaatg gttgtcgaag tacgcagtaa ataaaaaatc
     1141 cacttaagaa ggtaggtgtt acatgtccga gcttaatgaa aagttagcca cagcctggga
     1201 aggttttacc aaaggtgact ggcagaatga agtaaacgtc cgtgacttca ttcagaaaaa
     1261 ctacactccg tacgagggtg acgagtcctt cctggctggc gctactgaag cgaccaccac
     1321 cctgtgggac aaagtaatgg aaggtgttaa actggaaaac cgcactcacg cgccagttga
     1381 ttttgacacc gctgttgctt ccaccatcac ctctcacgac gctggctaca tcaataaagc
     1441 gttggaaaaa gttgttggtc tgcagactga agctccgctg aaacgtgctc ttatcccgtt
     1501 cggtggtatc aaaatgatcg aaggttcctg caaagcgtac aaccgcgaac tggacccgat
     1561 gatcaaaaaa atcttcactg aataccgtaa aactcacaac cagggcgtgt tcgacgttta
     1621 cactccggac atcctgcgtt gccgtaaatc cggtgttctg accggtctgc cagatgctta
     1681 tggccgtggc cgtatcatcg gtgactaccg tcgcgttgcg ctgtacggta tcgactacct
     1741 gatgaaagac aaatacgctc agttcacctc tctacaggct gatctggaaa acggcgtaaa
     1801 cctggaacag actatccgtc tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca
     1861 gatgaaagaa atggctgcga aatacggcta cgacatctct ggtccggcta ccaacgctca
     1921 ggaagctatc cagtggactt acttcggcta cctggctgct gttaagtctc agaacggtgc
     1981 tgcaatgtcc ttcggtcgta cctccacctt cctggatgtg tacatcgaac gtgacctgaa
     2041 agctggcaag atcaccgaac aagaagcgca ggaaatggtt gaccacctgg tcatgaaact
     2101 gcgtatggtt cgcttcctgc gtactccgga atacgatgaa ctgttctctg gcgacccaat
     2161 ctgggcaacc gaatctatcg gtggtatggg cctcgatggt cgtaccctgg ttaccaaaaa
     2221 cagcttccgt ttcctgaaca ccctgtacac tatggggccg tctccggaac cgaacatgac
     2281 cattctgtgg tctgaaaaac tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat
     2341 cgacacctct tctctgcagt atgagaacga tgacctgatg cgtccggact tcaacaacga
     2401 tgactacgct atcgcttgct gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt
     2461 cggtgcgcgt gcaaacctgg cgaaaaccat gctgtacgca atcaacggcg gcgttgacga
     2521 aaaactgaaa atgcaggttg gtccgaagtc tgaaccgatc aaaggcgatc tcctgagcta
     2581 cgatgaagtg atggagcgca tggatcactt catggactgg ctggctaaac agtacatcac
     2641 tgcactgaac atcatccact acatgcacga caagtacagc tacgaagcct ctctgatggc
     2701 gctgcacgac cgtgacgtta tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc
     2761 tgctgactcc ctgtctgcaa tcaaatatgc gaaagttaaa ccgattcgtg acgaagacgg
     2821 tctggctatc gacttcgaaa tcgaaggcga atacccgcag tttggtaaca atgatccgcg
     2881 tgtagatgac ctggctgttg acctggtaga acgtttcatg aagaaaattc agaaactgca
     2941 cacctaccgt gacgctatcc cgactcagtc tgttctgacc atcacttcta acgttgtgta
     3001 tggtaagaaa actggtaaca ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg
     3061 tgctaacccg atgcacggtc gtgaccagaa aggtgctgta gcgtctctga cttccgttgc
     3121 taaactgccg tttgcttacg ctaaagatgg tatctcctac accttctcta tcgttccgaa
     3181 cgcactgggt aaagacgacg aagttcgtaa gaccaacctg gctggtctga tggatggtta
     3241 cttccaccac gaagcatcca tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg
     3301 tgaaatgctg ctcgacgcga tggaaaaccc ggaaaaatat ccgcagctga ccatccgtgt
     3361 atctggctac gcagtacgtt tcaactcgct gactaaagaa cagcagcagg acgttattac
     3421 tcgtaccttc actcaatcta tgtaattaga tttgactgaa atcgtacagt aaaaagcgta
     3481 caataaaggc tccacgaaag tggggccttt tttagcacga gagccttttt tgtcagctat
     3541 ctatacttta aggtgactgc caaaacagac tcgacgtagc cttcgagctg cgcaccaaca
     3601 cggcctcaga tgggccacat ctggagaaac accgcaatgt cagttattgg tcgcattcac
     3661 tcctttgaat cctgtggaac cgtagacggc ccaggtattc gctttatcac ctttttccag
     3721 ggctgcctga tgcgctgcct gtattgtcat aaccgcgaca cctgggacac gcatggcggt
     3781 aaagaagtta ccgttgaaga tttgatgaag gaagtggtga cctatcgcca ctttatgaac
     3841 gcttccggcg gcggcgttac cgcatccggc ggtgaagcaa tcctgcaagc tgagtttgtt
     3901 cgtgactggt tccgcgcctg taaaaaagaa ggcattcata cctgtctgga taccaacggt
     3961 tttgttcgtc gttacgatcc ggtgattgat gaactgctgg aagtaaccga cctggtaatg
     4021 ctcgatctca aacagatgaa cgacgagatc caccaaaatc tggttggagt ttccaaccac
     4081 cgcacgctgg agttcgctaa atatctggcg aacaaaaatg tgaaggtgtg gatccgctac
     4141 gttgttgtcc caggctggtc tgacgatgac gattcggcac atcgcctcgg tgaatttacc
     4201 cgtgatatgg gtaacgttga gaaaatcgaa cttctcccct accacgaact gggcaaacac
     4261 aaatgggtgg cgatgggtga agaatacaaa ctcgatggtg ttaaaccacc gaagaaagag
     4321 accatggaac gcgtgaaagg cattcttgag cagtacggtc ataaggtaat gttctaattt
     4381 cctcgtaaag cggcaacttt attgagttgc cgctttttta ttccgcgctt cattctgcta
     4441 cagcgttttt aacacatctt atagtgcatc taccatatcc cgtacctgag ataataacga
     4501 ctgataatag ctcgcgtgaa gttcttcccc atcgaa
//
LOCUS       DAZPFP010000228         4489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPFP010000228 DAZPFP010000000
VERSION     DAZPFP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.231"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1151..1867
                     /gene="nanC"
                     /locus_tag="ACHUR4_003722"
     CDS             1151..1867
                     /gene="nanC"
                     /locus_tag="ACHUR4_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1457103.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1887..2993
                     /gene="nanM"
                     /locus_tag="ACHUR4_003723"
     CDS             1887..2993
                     /gene="nanM"
                     /locus_tag="ACHUR4_003723"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1457104.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3058..4038
                     /locus_tag="ACHUR4_003724"
     CDS             3058..4038
                     /locus_tag="ACHUR4_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1457105.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            4148..4353
                     /locus_tag="ACHUR4_003725"
                     /pseudo
     CDS             4148..4353
                     /locus_tag="ACHUR4_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1354 a          928 c          988 g         1219 t
ORIGIN      
        1 aagtaaaatc aacataaaaa attcagcaaa atttaatatt tgttaaacta gccgtctcaa
       61 atatgacatt attagttaac tgcatttgcc acaaaatggc tatttcatgg aacgcagtaa
      121 tttcttagtg gctattatca tgctaattct tagatcccta gcgattattc ctgcctggtt
      181 cgtcagctta ggccatgaga ttgttacatt cctttttatc ctggagatat gggggaacgc
      241 aggcgacgct ttttcatcta catccgttgg taaatatagc tttggtcact acgacttaag
      301 ccttcctcaa tcacagctat tcgccgggcg atacggcaaa caaactaagt gaaacatcat
      361 cctgaaaaca ccaacatcaa caagcctctc cagatcgaca tcaggagtga ccagttacaa
      421 gccacaaacc aagaaacata tttcatgatg agaattatgc tcagcctgat ggcgagtaaa
      481 cccttatttt caccactctc actgagttag cttgcaaagc tttctttgtt tagattctaa
      541 ataaattatt tataaaacgt atagttaaaa gaaaatcttt agtttttgaa gaccaccgca
      601 agtgttcgtc tggcttcaca tggcatcttc ttcttagaaa aagatcgaca tattttgtga
      661 cgcgaattgc aaatctggtt ttgttgtatg gattgcgtga tttttgatct ggtataacag
      721 gtataaagat gcaccacgat agtcaatgag actgggcatc tcgtaatcta tggcaaacat
      781 cacttcagtt ctttcacatc gggtgatgaa aatgcacttc agtctgaaag gaatatgaaa
      841 atgagatcaa cagacactct attttatgac tctgggtaaa atgggttgag taagtgatat
      901 aacttacgaa tattcaaatc aattaaaaat cagaagagat tttatactca ggtatttaat
      961 ctggatctct gtttatttaa ataatgttaa aagagaattt tcacaggaga ccttatacaa
     1021 aaaatataaa atacagttac ccgttgccaa agacactata agcctggcaa aaaaatatta
     1081 cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg
     1141 agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc
     1201 cccattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca
     1261 cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat
     1321 ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga
     1381 tgttcaggtt gaagttaatt acgcaattaa acttgatgat caatggacgg tgcgcccggg
     1441 aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg
     1501 ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg
     1561 acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt
     1621 cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca
     1681 gaatgattat cgctatgcaa accacaagaa atgggcgacg gaaaatgcct ttgttctaca
     1741 ataccatatg acgcccgata ttacgccata catagaatat gactaccttg accgccaggg
     1801 tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa
     1861 actgtagtag acaggagaca gtcacaatga ataaaactat aatggcgctt gctatcatga
     1921 tggcttcatt tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg
     1981 gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca ggtacagcat
     2041 ggtacaagct agatacacag gccaaagata aaaaatggac tgcgttagct gcattccctg
     2101 gtggaccgag agagcaagca acctctgcat ttattgatgg caatctgtat gtgtttggcg
     2161 gcattggcaa aaacagcgag ggattgactc aggtatttaa tgacgtacac aaatacaacc
     2221 ccaaaaccaa tagctgggtt aaattgatgt cgcacgcgcc gatgggcatg gcgggtcatg
     2281 tgacttttgt acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca
     2341 atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata gataaaatca
     2401 acgcccacta ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt
     2461 ttgatccctc aacacagcaa tggagttacg ctggcgaatc gccctggtac ggaacggctg
     2521 gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag
     2581 gattgcgaac ggatgccgta tttgaacttg atttcaccgg taataattta aaatggaata
     2641 agcttgatcc cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg
     2701 attctcttat atttgctgga ggggccggat tcaaaggttc acgagaaaat taccagaacg
     2761 gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc
     2821 ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat
     2881 tgccctggaa taatagttta ttaattattg gcggtgaaac tgcaggcggc aaagcggtga
     2941 cggattcagt tttaatttct gtgaaggata ataaagtcac agtacaaaac taacgcttca
     3001 gggccccggt aaaggggccc tgctatcaat ttgtcatttg tattacgcga aaattatatg
     3061 aacgcaataa tatcacccga ttattactat gttcttaccg ttgctggtca gtctaatgcc
     3121 atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca ttccagaatt
     3181 aaacaattag cgagatttgc gcatacgcat cccggaggcc cgccatgtca ctttaacgac
     3241 attattccac tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct
     3301 ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg
     3361 aaattactgc ccttcattcc tgataatgca gggattctta tcgttccgtg ttgccgtggc
     3421 ggatcggctt ttaccgcggg cagcgaaggg acatattcag aacggcacgg agccagccat
     3481 gatgcttgtc gttggggaac ggatactccg ctgtaccagg atttagtcag cagaacgcga
     3541 gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa
     3601 tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca tatggttgaa
     3661 gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac tgacgcgccg
     3721 tggttttgcg gcgataccac ctggtactgg aaagaaaatt tcccgcattc gtatgaagct
     3781 atttatggca attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa
     3841 caaggtgaaa gaggactgac gaacgcgcct gatgaagacc cggacgattt aagcacggga
     3901 tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat
     3961 ttcagcacgg cagcccgtcg ggggattatt tctgacagat ttgtagaagc aattttgcag
     4021 ttttggcgcg aaaggtgagc ggggagttta ttcatggggg atgtccttgt tcgttctcag
     4081 agtagacaac aaaaaacaaa tgagatcaag gtaatatctc ttcacttgcg ggctcgaaca
     4141 gcaggcaatt gccaatataa taatctggca caagggtgaa aaacgcgcac cgcataaacc
     4201 gttgttattg cttacagatt agccggatgc cttaatagac atccgcgcct tttcgattat
     4261 ggctcggaaa tctacgaaca tctacacagt ttactggaac gttttgggcc acagcgttcg
     4321 cagcaccgtc ctgatatgcc gttctggcga ttataagtcg acggattctg gcaaatagac
     4381 gatgatgata tgcttaatga tgttcaaaga aattaggcca ttattatatt attgcttgca
     4441 ttttctttca ttcctaaaag cacccgccat taatcccagg cgcattatg
//
LOCUS       DAZPFP010000229         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPFP010000229 DAZPFP010000000
VERSION     DAZPFP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.232"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(166..834)
                     /gene="artM"
                     /locus_tag="ACHUR4_003726"
     CDS             complement(166..834)
                     /gene="artM"
                     /locus_tag="ACHUR4_003726"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1457106.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(834..1550)
                     /gene="artQ"
                     /locus_tag="ACHUR4_003727"
     CDS             complement(834..1550)
                     /gene="artQ"
                     /locus_tag="ACHUR4_003727"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1457107.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1557..2288)
                     /gene="artI"
                     /locus_tag="ACHUR4_003728"
     CDS             complement(1557..2288)
                     /gene="artI"
                     /locus_tag="ACHUR4_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1457108.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2306..3034)
                     /gene="artP"
                     /locus_tag="ACHUR4_003729"
     CDS             complement(2306..3034)
                     /gene="artP"
                     /locus_tag="ACHUR4_003729"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1457109.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3252..3767)
                     /locus_tag="ACHUR4_003730"
     CDS             complement(3252..3767)
                     /locus_tag="ACHUR4_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1457110.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1153 a         1098 c         1073 g         1085 t
ORIGIN      
        1 gataacttag tgagttgtcc gtcttcatta taagaattta tgcactatgt aggccggata
       61 aggcgtccac gccgcatccg gcacaggcac cgtgctgatg tctgatgcga cgctggcgcg
      121 tcttatcaga cctacaaacc cccccggcga atgtacgcag ctacattaat tccgccgttc
      181 gaatgccagt gctttgcgct cgatcagacg catcatcagc gtcagcaggc cgttgacgac
      241 caagtaaata atccctgccg caccgaacac cattacatcg taggtgcgtc cgtacaacaa
      301 ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg tacttttgaa
      361 caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat acggcagcag
      421 gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc actgaccttc
      481 tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac tgttcagcga
      541 taacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagtg ccggatactc
      601 ctgcaacgtc ggaaactggc ccggcccgta ataaatcagg aagatctgca ccagcagcgg
      661 cgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg gcgttttcag
      721 cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc acaatcagcg aggcaacggt
      781 tagcgtcagg ctggtgtgca gccctttcat cagctcgggt aaatactcaa acattagctg
      841 ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta ctgactgagc
      901 agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta
      961 cgagtagcaa tgcttttggt ttgtaacatt aaatcattca cactaatcaa actgaccagc
     1021 gcggtatctt tcagcagcac cagccactgg ttaccgaggc caggcagcgc atgacgccac
     1081 atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag cgcctgacca
     1141 gattcccact ggcccaccgg tacagctttc aacgcgccgc gcagcgtttg cgaggcatag
     1201 gcggcataca gcagtgacag agcgatgaca ccacaaagga acggactcac gtcgaagttc
     1261 tcaatgtcca tctgcaccgg gatctgcacg aacccaagat tgatagtgaa gccatccgaa
     1321 agcgtcagca gtaactgcga ggagccaaaa tagataaaca gcactaccag aatttctggc
     1381 aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg ccatttcgct
     1441 gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc acaaacggca
     1501 aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat caggaattac
     1561 ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac tttttccagc
     1621 gcagtgttga gtttctgctg cagttcagtg tttccctgac gtaccgcgat gccgaggcca
     1681 gtaccgaaat aatctttatc ggtcacttta tcgcccactg ccgccagttt tgggttatct
     1741 ttcagccact cagtgaccac tgcggtgtca ccaaatacgc tgtcgatacg cccgttttgc
     1801 agatccagtt ttgcgttctg gtagctgtca tacggaacgg tggtgatttc cgggtgctta
     1861 tccataatga acttctggtg tgtcgtcccg ttctgaacgc cgactttttt gcctttcagc
     1921 tgatcaacac tggtgtattt gccttgctga cccacaaaca gggcagagtt gtcatagtac
     1981 ggggtagtaa acagtacctg cttttcacgc tccggagtga tatccatgcc cgccatcact
     2041 gcttctacgc gacggaattt caggcttggg atcaggctgt caaacgcctg gttagagaaa
     2101 gtacaggttg catcaatctc tttacacagt gcctgcgcca ggtcgacgtc aaaaccaacg
     2161 atctggttgt ttgcatcaat cgattcaaac ggaggatagg gggcttcggt agcaaaacga
     2221 atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc aatcagaact
     2281 tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat gcttcggttt
     2341 gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccattt tccatataca
     2401 ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc acctgggtaa
     2461 tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca
     2521 gtgcggcggt cggttcatcg aacagcagta cctgcggttc catcatcaac gcacgggcaa
     2581 tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt
     2641 tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct ttactcaacc
     2701 ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt
     2761 tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct ttgtcagagg
     2821 gtgttttggt gaaatcgaaa tggttgcctg caatgttgag cgtaccggag cgcggcatct
     2881 caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg ccaagtaaca
     2941 ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt
     3001 agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg gcagtctgat
     3061 agctattgag gtcgaagata gtacctttaa cagaataatt atgcaatatt tatgctttaa
     3121 aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg ggcggtggtc
     3181 ggtgagcata aatgtcggca ttcctcacga aatgccggat aatttacggg gtttattggt
     3241 tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgccgt ggcatgaacg
     3301 ctaccgccta agtagcgcac atcgtcgatg acccaacact ggccttcctg aatcattaac
     3361 acttcatcct gccagccctg gtcaccctgt ttgagatcca cccgcaatgg aatgttacgg
     3421 gcatcacgat tagggatagt cgatgcactg gcaacgtggg cgctgtctgg caaggtggtt
     3481 cgactggaga acggatcgct gcttaacaat tcacgatgac tattatcgcg gctggcatcg
     3541 ctaagttggg ttgccagttt gtcactcaaa taggggcgca gggcggtaat gtcattgctg
     3601 cgatgttgaa tgcggtagtc gtagaattgt tgagccacac tgtcaggccc tccctggaca
     3661 caggaaccgg tgcgggggcc gttatcttta taagctggcg tgacggtggt acaggcactc
     3721 agcagcagtg cacatggaat aagtagagat agtgaacggt agcgcataat catttcctta
     3781 ttagcttgct gtctgcgtca cactacgacc aaacgctatc agattattta atacattgtt
     3841 tttaatacaa ttctgaaaca atctgaacac ctgattgggt ttgagtccat tgctggcgac
     3901 ccactcaagg ttgctcgtag ctgacttact taaattaccc aaacaataga cagtgtaaac
     3961 catttgtgag gttgagcgtg ttatatggct gaaatgatca tttaaggtaa atatgatagt
     4021 tctgaaatgg tggtataaat cctcctgtag aggttaagag catctgcaag aagccagttt
     4081 cacatttata acgcgtgata aatgaatgcg gttattgtct aatcattaca aaatttttca
     4141 catgcgataa gagatcctgc gattaagatg attgccaaaa ttacgttgta tttttgaaaa
     4201 tgagcgaaat ttgatagaac atttatctgc tgtacgattt aatgaactta atgatacagc
     4261 attacaaaaa caatcgaagt ttataaagat gatttctgat tgaccagccc cctaccctac
     4321 aatggtgatt aattttatta ctcgcgatgg agatgatgat atgaatatat catatgtgaa
     4381 tagcaacaaa acgacatctt tacctgttg
//
LOCUS       DAZPFP010000230         4356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPFP010000230 DAZPFP010000000
VERSION     DAZPFP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.233"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..195)
                     /locus_tag="ACHUR4_003731"
     CDS             complement(<1..195)
                     /locus_tag="ACHUR4_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457111.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
     gene            complement(252..2612)
                     /locus_tag="ACHUR4_003732"
     CDS             complement(252..2612)
                     /locus_tag="ACHUR4_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1457112.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(2650..4335)
                     /locus_tag="ACHUR4_003733"
                     /pseudo
     CDS             complement(2650..4335)
                     /locus_tag="ACHUR4_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1185 a          972 c          967 g         1232 t
ORIGIN      
        1 caatgaaccg gtatgaattt gcagccataa atttacctga tcctttggat gagcatgcgg
       61 ataaatcata tatcgcaagt cattgtccag ttgcccggta attaactttt catcctgcgg
      121 taaggcggcg gcaatcagcc gcccggggag caacagagtt gccactaacg tcagtaagaa
      181 acagaggttt ctcataatta tctccatgcg aaaaccgggc gaatttaccc ggttaagtaa
      241 aatccgaact attaaaattt catgctgaca tcaagccaga aagtacggcc actggcgtag
      301 gtcgccacac cggtgttcgt tgtatcaaca gcggttttgc tatccagtac attgagaata
      361 tcggcactga tcgtcaggtt ttgttgtttc agaaattgtg gcgtccagga caagcgggtg
      421 tcccaggtca ggctgctatc cagtttttcg tcaacatact gcttgtaatc tgaatattcg
      481 ctgatgtatt gcgcatttgt cttaccaagg ataatgcgag ctttacgcgc ttcttgccag
      541 gccagcgtat tcgcccacac taacccgctc ggttgatgcg tgaaatccat gtttaaggag
      601 atctttaatg ggttattaaa atctgccact ggaacgctat cgtaagagac cagattaccg
      661 ttataaacca cctggttatc accggtattg ctctcctcat aaccattgtt taacgacaag
      721 ttgcccttgc tcttgatata gctaaagaca atttgcgggt taatatctac ctggcggata
      781 tgcaggggtt cggccagttc aaaactgagg ttaaacgaat gcgttttggt tttgccatcg
      841 ttgttatatt cagtaatggt ggttttagtc gcgctgtcgg tacgactgct tttgctgatt
      901 tgatcatgcg cttcacttgc gcgtgcaata acgttcttat cgattttttg ctgcaatccc
      961 attgccagtt catcgttata aggcgttttc aaattctgat aacgcgtcag ggttttatta
     1021 cctgataccg attccgtcca gctattgcgg atatcacgta atcccatatc aagaatattc
     1081 ccgccatagt aacggttata accggcggta atcattgagg tttgatcagc aaaaatatcc
     1141 cattccgtca taaagcgcgg ggagatattg tggtttgaca gatagttgtc atagtcataa
     1201 cgcacaccgg gcattaacga cacattccgc cagctaatgt gatccgccat atacagcgtg
     1261 tagttgtcaa ttcccaggct gcctttacct ttatggtaaa tggtatggtt agttttcttt
     1321 cctgcagcat taatcacata ggattcagac tggttatggc gttcagtcca tgcatcggaa
     1381 tagatgtatt ccgcgccgaa gtagggttga tgcgaaacat cacccacggc gaatttttgc
     1441 cagtccaggc gtgttttgaa ggtgtaatta tctacagcct gggaaatgtg tcctaatccg
     1501 ccacgggtac aacgccctgt aatatcacca tatgtacatg aaagttcggt gtaccagatg
     1561 tcatgatcgt gacgggtata atcactaata tgatcccaac caacggtggt acgtagtttg
     1621 gcccatgcga gttgcgtatc catatcccat gccagaccat aggatttatt acccatttcg
     1681 cgatcagact gcgggaaggt gctggtatta taatcacggc tggagccggt atattttaag
     1741 gttaaatcgt gggtaaagcg gtcgctggca aaccaggtaa atttgctcaa tgcagtatcg
     1801 ataacgtttt tatactgtgc ccgaccggcg acaatgccgt cattcgaaac ataatccgcg
     1861 cgggtgatat cagactggcg gcgcgataaa ccggcggtaa cgccaaagtt atcagccagc
     1921 tcctgattaa acgacaaggt ataaaagttc tttttaaaat ctggagagaa ataggtactt
     1981 cctgaagaac cttgattaaa tgcgctcttg ttattctcat cgatatgcga tgttaaccag
     2041 tccaaacgcg tagtgcgata acccaatttc accttgctat catcagcgtt gaagcgtttg
     2101 atctttgcat caattacccc gccattgaag cgaccaaatt caaccggcac aaaactgtca
     2161 taaagcgtca cattgtccag taagctgaca tcaagataat acccctgtga catcccgcta
     2221 atattggttg cactactggc atcggactca ttcgctgggt tcaggttatt agttgcacta
     2281 ataccgtcaa tcaaataggc attctggtag ggcgacgcac cgtgaataga gattttctca
     2341 gggcgaatat ctccctggtt caacgaggta ctttgcgttg aatccatgcg taccgcaggg
     2401 ttggttctca gcagatcgct gatattgcca ttcccggtcg gtaaacgctc gatgctttca
     2461 ctggtgtaat gcgtgttgcc gtttacgggt acagggacag gagcatagac cgtcatctct
     2521 tcaaaaaaat acatgtacat gtttttgtca tcgacggcct gcgccacatc agcgccacat
     2581 aaaatgacgc caggaataag aactcgcttc atttatttat atctccgtac agcagggctt
     2641 ttattgttgt tataaaaccg tgctaatgtc acaaatatca tcagccaggt tccagacacc
     2701 gggttgatgt gtaaccataa tgacgccgct tgtgggtagt ttttcacgca ctaaacgcag
     2761 taagcgtata gcctcttgtt cctcaagatg agaggtagtt tcgtcaagaa atatccattt
     2821 cggacgtcgt aaaattaatc gtgccagggc gatacgttgt ttttcgccgc tggaaagaat
     2881 atctccccag cgatcgtggt catgaatacg cgcagccaat tttcccagac caacctgatg
     2941 cagtacttcg ctcaacgatt tatcgtctac gggcagggga agtgctttac aaataatctc
     3001 tttcagtaag ccgcttttga ttaacggtgt ttgtgacaca taccaactgt cagcaggaga
     3061 agaaatatca cctttaaacc acggccagca gtgggataat gttttaagca gtgtggtttt
     3121 tcccgcacca gagtagcctt tcagtaatag ccatttgcct ggcgaaacat gaaagttcaa
     3181 gttctctaat atgatcttat tatcaggcgt acgaatactc gcattagcca cttgtaccgc
     3241 atgttggcaa tttttaggct tattcgtagg gcgctattca gtgagttgat ggaactcata
     3301 caagcgatcg ataaccgcag ccagttcagc aagttcgtca tatttataaa taaaccagct
     3361 taaattgttc gatactagca taaatgcctg gcgcgatttc atcagtccgc ccagattgat
     3421 ctgcccgcta ataaactgcg gcaataacag aaagtaggga agaacgctaa gcgagcgcga
     3481 ataaatattt tgccagtaat caagccaccg ctgacgattc attaaacgat gccagttctc
     3541 tttaatggta tgaaaattgt cgcgcaactc ctggcgttgt agagactcag cattgcttag
     3601 cgcaatcagt tctgcctgct tattatgctg cacaagatta gttcgaaatg tcgcttcgct
     3661 acgttgtttt tccacattaa gcggacgaat acgtttaccc accttatggg taaataaagt
     3721 tccaccgatc acaatgagca cgacggtata gaccatatac ccctggatat tccattctgt
     3781 tccaccaaca gtgaatgaga gcgtacccgc gctttgccaa agaataacgg taaaggtgat
     3841 cagcatgcta agtgactgga tgaagccaaa tgaaagactg agcgttttgt tgatcagtaa
     3901 gagaatgtct tcagcgatac gttggtcagg gttatctgta tttttatgct cgccgtagat
     3961 ctgcgtgaag tagtagtttt tatctgcaaa ccagcgatta aggtagtaat ctgttaacca
     4021 ttcgcgccag cgaatagtca gtagtttaat taaccatgtt ttgtttgcgg atattaatac
     4081 aaagatcccc agcagggcag ggaaccaaag aacgagctgc caaagcttat cggtttcttt
     4141 ctggcttagc gcattgaaaa aatcattatt ccagtcgttt aaccagacct gaattttaac
     4201 cacgccaagg atcattgcga ggataataat gattaacaac actgaagttt tattattctt
     4261 acgcagccaa aagggcttca gcagacagag atactttgct atcaacatac aaaacgtaat
     4321 gggaatggtt atcattagcg aaaattgatt gtgcca
//
LOCUS       DAZPFP010000231         4318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPFP010000231 DAZPFP010000000
VERSION     DAZPFP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.234"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(202..948)
                     /gene="cutC"
                     /locus_tag="ACHUR4_003734"
     CDS             complement(202..948)
                     /gene="cutC"
                     /locus_tag="ACHUR4_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1457113.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(962..1528)
                     /locus_tag="ACHUR4_003735"
     CDS             complement(962..1528)
                     /locus_tag="ACHUR4_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1457114.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1744..3477
                     /gene="argS"
                     /locus_tag="ACHUR4_003736"
     CDS             1744..3477
                     /gene="argS"
                     /locus_tag="ACHUR4_003736"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1457115.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3519..3563)
                     /locus_tag="ACHUR4_003737"
     CDS             complement(3519..3563)
                     /locus_tag="ACHUR4_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457116.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3654..4142
                     /locus_tag="ACHUR4_003738"
     CDS             3654..4142
                     /locus_tag="ACHUR4_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1457117.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1146 a         1067 c         1044 g         1061 t
ORIGIN      
        1 tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag cacaacgcta
       61 tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg
      121 ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc
      181 gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc
      241 agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga
      301 catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct
      361 atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc
      421 tccagccata atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc
      481 ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag
      541 attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc
      601 cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac
      661 atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc
      721 aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat
      781 cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt
      841 taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc
      901 cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta
      961 attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag
     1021 ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg
     1081 gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca
     1141 gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac
     1201 cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa aactgccagt
     1261 gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcattt
     1321 tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca gcggtgacgt
     1381 tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac
     1441 ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca
     1501 gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa
     1561 tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt
     1621 gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc
     1681 cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt
     1741 ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc
     1801 gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt cggcgactat
     1861 caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca
     1921 gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt
     1981 ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg
     2041 ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac
     2101 tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt
     2161 gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc
     2221 ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa
     2281 aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat
     2341 tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc
     2401 ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac
     2461 cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc
     2521 ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta
     2581 gaaagcgaag gggcgaccgt cgtattcctt gatgtgttta aaaacaagga aggcgaaccg
     2641 atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc
     2701 tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc
     2761 cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg
     2821 gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg
     2881 ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga agccctggag
     2941 cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa
     3001 ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc
     3061 acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac
     3121 atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat taacgaagag
     3181 caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc
     3241 ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg
     3301 tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc
     3361 ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg
     3421 aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg
     3481 atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt attgccatct
     3541 aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag aggccagaag
     3601 cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc gaagtgttta
     3661 aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta
     3721 tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat tcagatgatg
     3781 atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc
     3841 aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg
     3901 caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg
     3961 attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag gatttaagtg
     4021 acccctattc tgaatcagaa tggctatcat gtatgatcat acagaccaat acgcgaactc
     4081 gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat
     4141 aattcacatc tttttggcat gttttgttgc aagctattcc tgataaatga ttgcaacaaa
     4201 acatccagcc ttttgactga gttattaaac atactcgcga gcgcgtaatt ttttgtcttt
     4261 cagcgataat tcacaatcac ttcattccgt tgtaccttta gcggtggaat taaccgcc
//
LOCUS       DAZPFP010000232         4312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPFP010000232 DAZPFP010000000
VERSION     DAZPFP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.235"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUR4_003739"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUR4_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1457118.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHUR4_003740"
     CDS             1131..1931
                     /locus_tag="ACHUR4_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1457119.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHUR4_003741"
     CDS             2009..2779
                     /locus_tag="ACHUR4_003741"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1457120.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUR4_003742"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUR4_003742"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1457121.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..>4312)
                     /locus_tag="ACHUR4_003743"
     CDS             complement(4245..>4312)
                     /locus_tag="ACHUR4_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457122.1"
                     /translation="ETLLQQPEERFAWLRSKKDRF"
BASE COUNT         1032 a         1087 c         1056 g         1137 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc tt
//
LOCUS       DAZPFP010000233         4263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPFP010000233 DAZPFP010000000
VERSION     DAZPFP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.238"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            218..1150
                     /gene="rfaD"
                     /locus_tag="ACHUR4_003744"
     CDS             218..1150
                     /gene="rfaD"
                     /locus_tag="ACHUR4_003744"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1457123.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1160..2206
                     /gene="rfaF"
                     /locus_tag="ACHUR4_003745"
     CDS             1160..2206
                     /gene="rfaF"
                     /locus_tag="ACHUR4_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1457124.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2210..3202
                     /gene="rfaC"
                     /locus_tag="ACHUR4_003746"
     CDS             2210..3202
                     /gene="rfaC"
                     /locus_tag="ACHUR4_003746"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1457125.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3199..>4263
                     /gene="rfaL"
                     /locus_tag="ACHUR4_003747"
     CDS             3199..>4263
                     /gene="rfaL"
                     /locus_tag="ACHUR4_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR1457126.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKN"
BASE COUNT         1122 a          966 c         1048 g         1127 t
ORIGIN      
        1 cgttatttat catacatcgg aatattgata ctaaagcact attcacatgc aaaaccaaca
       61 tccgccatga aggactagct aaaacccaaa ctagtttgtt gcaattagca tccttgcacc
      121 tctatgtaaa gggctgaagg gattcggatg tgatggtatg attacagaca ttcgtgtctg
      181 agattgtctc tgactccata attcgaaggt tacagttatg atcatcgtta ccggcggcgc
      241 gggctttatc ggcagcaaca tcgttaaggc cctgaatgat aaaggcatca ccgatattct
      301 ggtggtggac aacctgaaag acggcaccaa gtttgtgaac ctggtggatc tggatatcgc
      361 ggactatatg gataaggaag acttcctgat ccagattatg gctggcgaag agttcggtga
      421 tgtcgaagcg attttccacg aaggcgcatg ctcttccacc accgagtggg atggcaagta
      481 tatgatggat aacaactatc aatactccaa agagctgctg cactactgtc tggagcgcga
      541 aatcccgttc ctgtatgcct cttccgcagc cacctacggc ggacgcacct ccgactttat
      601 tgaatcccgc gagtacgaaa aaccgttgaa tgtctacggt tactcaaaat tcctgtttga
      661 tgaatatgtt cgtcaaatcc tgccagaagc gaactcgcag attgttggct tccgctattt
      721 caacgtttat ggaccgcgtg aaggccataa aggcagcatg gcgagcgtcg ctttccatct
      781 caacacccag cttaataacg gtgaatcacc gaagctgttc gaaggtagcg agaacttcaa
      841 acgtgacttc gtctatgtgg gcgacgtggc agatgtaaac ctgtggttcc tggaaaatgg
      901 cgtttccggt atcttcaacc tcggcaccgg tcgtgcagaa tccttccagg cagtagcaga
      961 tgctacgctg gcttatcaca agaaaggcca gatcgaatac attccgttcc cggataagct
     1021 gaaaggccgc taccaggcgt tcactcaggc agatctgaca aatctgcgcg cggcgggtta
     1081 cgacaaaccg ttcaaaaccg ttgctgaagg tgtaacggaa tacatggcct ggctgaatcg
     1141 cgacgcataa gagctctgca tgaaaatact ggtgatcggc ccgtcttggg ttggcgacat
     1201 gatgatgtcg caaagtctct atcgcacgct ccaggcgcgc tatccccagg cgataatcga
     1261 tgtgatggca ccggcatggt gccgtccatt attatcgcgg atgccggaag ttaacgaagc
     1321 tatccctatg cctctcggtc acggagcgct ggaaatcggc gaacgccgca aactgggtca
     1381 tagcctgcgt gaaaagcgct acgaccgcgc ctacgtctta ccaaactcct tcaaatctgc
     1441 attagtgcct ttcttcgcgg gtattcctca tcgcaccggc tggcgcggcg agatgcgcta
     1501 cggtttactc aacgatgtac gcgtgctcga taaagaagcc tggccgctaa tggtggaacg
     1561 ctatgtcgcg ctggcctatg acaaaggcat tatgcgtacc gcacaagatc tgccgcagcc
     1621 attgttatgg ccgcagttgc aggtgagcga aggtgaaaaa tcatatacct gtaatcaatt
     1681 ttcgctttca tcagaacgtc cgatgattgg cttttgcccg ggtgcggagt ttggtccggc
     1741 aaaacgctgg ccacactacc actatgcgga actggcaaag cagctgattg atgaaggtta
     1801 tcaggtggtt ctgtttggct ctgcgaaaga tcatgaagcg ggcaatgaga ttcttgccgc
     1861 tttgaatacc gagcagcagg catggtgtcg gaacctggcg ggggaaacac agcttgatca
     1921 agcggttatc ttgattgcag cctgtaaagc cattgtcact aacgattctg gcctgatgca
     1981 cgttgcggcg gcgctcaatc gtccgctggt tgccctgtat ggtccaagta gcccggactt
     2041 cacaccgccg ctatcccata aagcgcgcgt gatccgtctg attaccggct atcacaaagt
     2101 gcgtaaaggt gacgctgcgg agggttatca ccagagcttg atcgacatta ctccccagcg
     2161 cgtactggaa gaactcaacg cgctattgtt acaagaggaa gcctgacgga tgcgggtttt
     2221 gatcgttaaa acatcgtcga tgggcgatgt tctccatacg ttgcccgcac tcactgatgc
     2281 ccagcaggca atcccaggga ttaagtttga ctgggtggtg gaagaagggt tcgcacagat
     2341 tccttcctgg cacgctgccg ttgagcgagt tattcctgtg gcaatacgtc gctggcgtaa
     2401 agcctggttc tcggccccca taaaagctga acgcaaagcg tttcgtgaag cgctacaagc
     2461 agagaactat gacgcagtta tcgacgctca ggggctggta aaaagcgcgg cactggtgac
     2521 acgtctggcg catggcgtaa agcatggatt ggactggcaa accgctcgcg aacctttagc
     2581 cagcctgttt tacaattgta agcatcatat tgcaaaacag cagcacgccg tagaacgcac
     2641 ccgcgaactg tttgccaaaa gtttgggcta tagcaaaccg caaacccagg gcgattatgc
     2701 tatcgcacag cattttctga cgaacctgcc tacagatgct ggcgaatatg ccgtatttct
     2761 tcatgcgacg acccgtgatg ataaacactg gccggaagaa cactggcgag aattgattgg
     2821 tttactggct gattcaggaa tacggattaa acttccgtgg ggcgcgccgc atgaggaaga
     2881 acgggcgaaa cgactggcgg aaggatttgc ttatgttgaa gtattgccga agatgagtct
     2941 ggaaggcgtt gcccgcgtgc tggccggggc taaatttgta gtgtcggtgg atacggggtt
     3001 aagccattta acggcggcac tggatagacc caatatcacg gtttatggac caaccgatcc
     3061 gggattaatt ggtgggtatg ggaagaatca agtagagtgt cgttctacaa gtatgtctct
     3121 tgcagatttg ccagctcaaa ccgtttttca aaatttaaat cttgaaataa taaccaataa
     3181 gttgacatcg gagataagat gacctcaaca ttatttttct ctctcgagaa aaaaaactgg
     3241 atagcgtact ggaacagagc tctcgtattc ttattcatta ccacctattt tttgggtggg
     3301 attacaaggt ataaacatct tattgttatt cttatgacaa taacgacaat cgtctatctc
     3361 tgcaaacggc caaaacacta tctctcacta tttaaaacat ttctttttgg tagtgttgcc
     3421 atattaacta ttgctgcatt gctgtcactt cttcaatccc ctgatgcagg tgctagcatg
     3481 aaggaagttt tcaaagctat tattgagaat actttactat gcacaatagc aataccggtc
     3541 atattgagag acgagaaaag agaagatgtc gaaaaaatcg ttttcttctc atttattagt
     3601 gcgttgggct tacgctgttt ttctgaattg attacctatt ataaggacta tcaacaaggg
     3661 ataatgccat tcgcagatta tagacaccgt agcatttctg actcgatggt ctttttattc
     3721 cctgcattgt taaatctctg gcttatcaaa tcagcaaaat accgcatttc ttttgtggtt
     3781 ctaagcgtta tttttatttt tctgatatta ggaactttat ccagaggggc ctggctttcc
     3841 gtgttagtca ttggattaat atggattctg atgtttaaac aatggaagtt actattagta
     3901 ggagtaatgg tttccatcat tgcattgtcg gttattttca cacataagga gatgaccgca
     3961 aagctaacgt ataaacttca acaaactaat agttcttatc gctatgcaaa tggtactcaa
     4021 ggcagcgcac tcgatctaat attagaaaat cctgttattg gttatggtta cggtaacgtt
     4081 gcatataaag atgtctataa taaacgtgtc attgattatc cagaatggac ctttagacaa
     4141 tcaatagggc cacataattt tgcgctattc atctggtttg gcactggttt attagggctg
     4201 gtaagtctta tgatgctata ctgtgcaata ttgaaagagt gtataaaaaa tggcgtcaag
     4261 aat
//
LOCUS       DAZPFP010000234         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPFP010000234 DAZPFP010000000
VERSION     DAZPFP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.239"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUR4_003748"
     CDS             complement(<1..209)
                     /locus_tag="ACHUR4_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1457127.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHUR4_003749"
     tRNA            complement(324..399)
                     /locus_tag="ACHUR4_003749"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHUR4_003750"
     tRNA            complement(406..480)
                     /locus_tag="ACHUR4_003750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHUR4_003751"
     tRNA            complement(597..681)
                     /locus_tag="ACHUR4_003751"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHUR4_003752"
     tRNA            complement(690..765)
                     /locus_tag="ACHUR4_003752"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUR4_003753"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUR4_003753"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1457128.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUR4_003754"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUR4_003754"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1457129.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUR4_003755"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUR4_003755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1457130.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZPFP010000235         4203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPFP010000235 DAZPFP010000000
VERSION     DAZPFP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.240"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(34..360)
                     /locus_tag="ACHUR4_003756"
                     /pseudo
     CDS             complement(34..360)
                     /locus_tag="ACHUR4_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(370..918)
                     /locus_tag="ACHUR4_003757"
     CDS             complement(370..918)
                     /locus_tag="ACHUR4_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1457131.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(918..1625)
                     /locus_tag="ACHUR4_003758"
     CDS             complement(918..1625)
                     /locus_tag="ACHUR4_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1457132.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACHUR4_003759"
     CDS             complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACHUR4_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1457133.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2322..3032)
                     /locus_tag="ACHUR4_003760"
     CDS             complement(2322..3032)
                     /locus_tag="ACHUR4_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1457134.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACHUR4_003761"
     CDS             complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACHUR4_003761"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1457135.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         1071 a         1075 c         1067 g          990 t
ORIGIN      
        1 aatgccagtc agttgcttaa ctgactggca ttactctttt aaggtgcgga agttttcgcc
       61 gttcacccag agcagatcca ccgagccgcc cgttttacga ccggcagcag cttcggtctg
      121 aatgcgcttc accgcgtctg cggcatccgc gagacgaaca atcttcaggt ttatagcgta
      181 atgggttttc atctcgccgc taacccagtc gagatagcgg ttaattgcgg tatcgccgcc
      241 ccaggcgtta aaccagacgg tttgcccttt ggcctcattt ttaatttcct gccagtcact
      301 ggcatgtgtt gccaaagaaa acagcgataa caatcccagc aaccacccac aatggcgcat
      361 cttccctcct taagaacgtt tttccggaat acggcacagc ccctgagcgg gaatgtccat
      421 ggcagaatta aaacgagccg acaggttttg caggccgatc aatgccgtca gttcggtgag
      481 cgcctgagcg tcaaaatgcg cagccagtcg ggtacgcagg gcatcatcga ctgttggcgg
      541 tgtgacgctt gcggcttcgg cgtactccag cgccagccgt tcttcatcgc taaagagcgg
      601 gctttgccgc cagtcagcca ctgccagcag tttatcggtg ctgccggtac gctcggcgac
      661 tttcatactg gtgatatcca cgcaaaactc acacaggcac atttgcgcaa tgcgcgcgct
      721 gacaagcgat cgcactaccg gatcgagcgg tgagcgtttg cgctccagcc acccaacaaa
      781 cattgacacc aaataaaaga taaacgggat ccgcccccac cagcgaatcg ggcttagcac
      841 ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt aaaatgaaag gaattttgct
      901 aagcggcggt aaacccatca gtcattcctc ttctggcgct caagccatat tttcttcgcc
      961 ataaaaatca ccacggtcag ggcaaacaga ataaacagtt cggtgacaaa ccagaaagta
     1021 ccgcctgtta acatgctgcc tgcccaggaa taaacaatag tcgccggtaa ttgaccaagc
     1081 ccggtggcga taaaaaacga gcgaaaacgt attgaagtca gaccggcagc atagctgatt
     1141 ggatcgaaag ggacaaaagg caataaccga cagaccagga tggtgtgttt gccgtagcga
     1201 gtgaaaaagc cgtccatact gtcaagcacg gttttgccgg ttaatttttc caccacttcg
     1261 cggcccatca ctctggcgat aaaaaagcac agcgccgcgc cggccatcga actggtccac
     1321 gacagcaagc cgccccagaa cgcgccaaac agcgacgcat tggcaaaggt gatcaaaaac
     1381 gcaggtagcg gtgcagcaat ggcctgtaaa atcatcaaca agaacgagac aactgccgcc
     1441 agtgcgccgt aagactgaat aaagcgttct ataccctgtt ggtccacggc ggcaaacgcc
     1501 gcaacgctgc gattgataaa ctcatggacg ccgggaagca gcgcccatgc gagcagcata
     1561 gcgaacaaca ggatgatgag ggttatacgg tagtaccaga ttttacgcga ttgtatcttc
     1621 atcatccttt atcttggctt tattcggtga ttttaaaggt aaccgttgcc agcgtgttat
     1681 ccgccgggag aactgaggca ttgcctttga atttcacttc gccgtgtttt tccaccgcac
     1741 cataagtgta tgttgcattg ctgacgatgc cgaccgggca gctatccaga cacaccaggc
     1801 aacccgtttt cttctcttct gctgccgtta aattaccgcc aaagcgcatg tccagtttct
     1861 tgccattact gtcaacaatc acttcatcga aggaatacgc ttttgccgcc ccttgccagt
     1921 tgaccgaaat atccagtttg ctgcctgtga catgagtcgt ttctttatta tccatcgtca
     1981 tgttttcgcc cggcgtacca cctgcctctt tcagggcttc ataaaatgct ttcgggggcg
     2041 cataagccat aaacagcgat ttatgtccgt tggagccatc tttaaacaca ataccgtgac
     2101 gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag catggttacc gtatggttat
     2161 tggcatcgac gcgcagcggt tggctgttcg aaagtccgtc gtattccact tttgccgccg
     2221 cattctcttt ttgatcgcag ccgctaagcc cggcaacagc cagtaagcag agtgcagcca
     2281 gtcctttttt ccatgacact gaataatgtt gcgacatcgt tctatccttc attttttggg
     2341 tgagtaagtg ggctgcggcg cgaagcagac agatccacat gtttgtgtcg ggcgtagagt
     2401 tttgcgagct ggatgagaat aaacagcgcc aggcccgcca gacagagttg taaaataaag
     2461 cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca cggtataaat aacaataccg
     2521 ggcagggtcg tgagtgccga aataagggta taaggccaga aggcgatggt ggtgaatccg
     2581 taagcgtaat tttgaatatt gtaaggaaac aacgggatta agcgggtcag aataagaaaa
     2641 tcaataccgt tacgcgcaat gcctttttca atggcctgga aggtatggct atggcccacg
     2701 tatttcagca gtaaatcacg tcccagccag cgcgccagca ggaatgagca cgaggaggcc
     2761 agcgttgcgg caattaatga gagttgtgtc cctaagagcg ggccaaaaac tattcctcca
     2821 gcgatcacca gtatgcttcc tggtaacaga aagagggggg cattgatgaa taacagaata
     2881 tagaggctat aaccgaacaa tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg
     2941 gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga
     3001 caggcaaaca gaaagttacg ctcagcgttc atcgtggagt ttcctatatc aaacttgata
     3061 atagagaagt ggtcagacaa ctaaaaacgc agtgacggta taacaaagga cggcaggcaa
     3121 caaatcatta ctgacagttt gttgattttt ctcaaggtta attctcctga cccagtttga
     3181 gccaggagag ctgctaaatt agcggcggaa ggtcgcccag acgggggcgt gatcggacgg
     3241 tttttccatg ctgcgaattt catagtcgat gccggtttct acgcaacatt ctgccagcgg
     3301 ttggctggcg agcagcaggt cgatgcgcag accacggtta tcgtcaaaac cttttgagcg
     3361 gtaatcaaac catgagaaac gatctgctgt ttgcggatta gcatggcgga aggtatcgac
     3421 caccccccag ctcatcagcc tgtccatcca ttcgcgctct tccggcagga aagagcattt
     3481 accggtacgc agccagcgct tacggttttc ttcgccaatg ccgatatcca gatctgtagg
     3541 gctgatattc atatcgccca taatcagtac cggattatca cgtttgagtt cggtttccag
     3601 gtagttttgc agattctgat aaaactgcgc ttttgccggg aattttatcg gatggtcgcg
     3661 gctttcaccc tgcgggaagt aaccgttgat cacggtgaca ttacccagca gtgaggggat
     3721 ttccgccata ataatccgcc gctgcgcctc ttcgtcgtca ccgggaaagc cgcgacgcac
     3781 ggcaatcggc gtctctttgg tcagcagcgc cacgccataa tggcctttct gcccgtgata
     3841 aaacacgttg tagccgagct tcgccacctc ttcgagcgga aacatatcgt catgaacttt
     3901 tgtctcctgc aggccaatca catccggttg gtgcttttcg acgatggctt caagctggtg
     3961 aggtctggcg cgcaggccgt tgatattaaa agagacaaat ttcatagtcg ctgccattta
     4021 ttcagataaa gagtgcgtgg atggtagcag aatttcgcgt tgcttaccgc ctgttgttaa
     4081 gtgatttgat cgatggttgt taatgatgtc aacgatggcg caaaaaatgc ccgcttttgg
     4141 tgcgcgctgc gtcagaatgg cgcagtaatt tccagtaaat ttcgtcaatg atcacaattc
     4201 tag
//
LOCUS       DAZPFP010000236         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPFP010000236 DAZPFP010000000
VERSION     DAZPFP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.241"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHUR4_003762"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHUR4_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1457136.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHUR4_003763"
     CDS             complement(995..1690)
                     /locus_tag="ACHUR4_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1457137.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHUR4_003764"
     CDS             complement(1683..3110)
                     /locus_tag="ACHUR4_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1457138.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHUR4_003765"
     CDS             complement(3121..3840)
                     /locus_tag="ACHUR4_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1457139.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZPFP010000237         4189 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPFP010000237 DAZPFP010000000
VERSION     DAZPFP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.242"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHUR4_003766"
     CDS             <1..297
                     /locus_tag="ACHUR4_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1457140.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHUR4_003767"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHUR4_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1457141.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHUR4_003768"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHUR4_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1457142.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHUR4_003769"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHUR4_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1457143.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHUR4_003770"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHUR4_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1457144.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1128 a         1035 c         1035 g          991 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcg
//
LOCUS       DAZPFP010000238         4186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPFP010000238 DAZPFP010000000
VERSION     DAZPFP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.243"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUR4_003771"
     CDS             <1..190
                     /locus_tag="ACHUR4_003771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457145.1"
                     /translation="IQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITN
                     IISIILQQAQDYDVAKIT"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="ACHUR4_003772"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="ACHUR4_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1457146.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1026..1079)
                     /gene="ychE"
                     /locus_tag="ACHUR4_003773"
     CDS             complement(1026..1079)
                     /gene="ychE"
                     /locus_tag="ACHUR4_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR1457147.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="ACHUR4_003774"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="ACHUR4_003774"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1457148.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          962 g         1035 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttctgg
//
LOCUS       DAZPFP010000239         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPFP010000239 DAZPFP010000000
VERSION     DAZPFP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.244"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUR4_003775"
     CDS             complement(<1..186)
                     /locus_tag="ACHUR4_003775"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1457149.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUR4_003776"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUR4_003776"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1457150.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUR4_003777"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUR4_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1457151.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHUR4_003778"
     CDS             3863..4144
                     /locus_tag="ACHUR4_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1457152.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZPFP010000240         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPFP010000240 DAZPFP010000000
VERSION     DAZPFP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.245"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUR4_003779"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUR4_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1457153.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUR4_003780"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUR4_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUR4_003781"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUR4_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHUR4_003782"
     CDS             3787..>4168
                     /locus_tag="ACHUR4_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR1457154.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZPFP010000241         4058 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPFP010000241 DAZPFP010000000
VERSION     DAZPFP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.246"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            172..3480
                     /gene="icsA"
                     /locus_tag="ACHUR4_003783"
                     /gene_synonym="virG"
     CDS             172..3480
                     /gene="icsA"
                     /locus_tag="ACHUR4_003783"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR1457155.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3624..3942
                     /locus_tag="ACHUR4_003784"
                     /pseudo
     CDS             3624..3942
                     /locus_tag="ACHUR4_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1220 a          703 c          963 g         1172 t
ORIGIN      
        1 tttttcaaag caagacacag gtaaatttct cccgttgcat tgatatataa cacagctctc
       61 atgttttggt tgaggctttg tttaatatgt ttttgcatat atcgtccctt tattccggat
      121 aaaacggaat cttttcaggg gtttatcaac cacttactga taatatagtg catgaatcaa
      181 attcacaaat ttttttgtaa tatgacccaa tgttcacagg ggggggccgg agaattacct
      241 acggtaaagg aaaaaacatg caaattgtct ttttctcctt ttgttgttgg tgcatccctg
      301 ttgctcgggg ggccaatagc ttttgctact cctctttcgg gtactcaaga acttcatttt
      361 tcagaggaca attatgaaaa attattaaca cctgttgatg gactttctcc cttgggagct
      421 ggtgaagatg gaatggatgc gtggtatata acttcttcca acccctctca tgcatctaga
      481 actaagctac ggattaactc tgatattatg attagcgcag gtcatggtgg tgctggtgat
      541 aataatgatg gtaatagttg tggcggtaat ggtggtgact ctattaccgg atctgacttg
      601 tctataatca atcaaggcat gattcttggt ggtagcggcg gtagcggtgc tgaccataac
      661 ggtgatggtg gtgaggctgt tacaggagac aatctgttta taataaatgg agaaattatt
      721 tcaggtggac atggtggcga tagttatagt gatagtgatg gggggaatgg aggtgatgcc
      781 gtcacaggag tcaatttacc cataatcaac aaagggacta tttccggtgg taatggaggt
      841 aacaattatg gtgagggtga tggcggtaat ggaggtgatg ccatcacagg aagcagcctc
      901 tctgtaatca ataagggcac gttcgctgga ggcaacggag gtgctgctta cggttatggt
      961 tatgatggct acggtggtaa tgctatcaca ggagataacc tgtctgtaat caacaatgga
     1021 gctattttag gcggtaatgg tggacattgg ggggatgcta taaatggtag caatatgacc
     1081 attgctaata gcggatatat aatttcaggt aaagaagatg atggaacaca aaatgtagca
     1141 ggtaatgcta tccacatcac tggtggaaac aattcattaa tactccatga aggttctgtc
     1201 attactggtg atgtacaggt taacaattca tccattctga aaattatcaa caatgattac
     1261 actgggacca caccaactat tgaaggtgat ttatgtgctg gtgattgtac aactgtttca
     1321 ctatcaggta acaaattcac tgtttcaggt gacgtttctt ttggtgagaa cagttcttta
     1381 aatttagctg gaatcagtag tctggaagct tctggaaata tgtcatttgg caacaatgta
     1441 aaagtggagg ctattataaa taactgggcg cagaaggact ataaactgct aagtgcagat
     1501 aaagggataa caggtttcag tgtttctaat atatctatca tcaatccgtt actcactact
     1561 ggtgctattg actatacaaa aagctatatc agtgaccaga ataaattgat ctacggtttg
     1621 agctggaatg atacagatgg cgacagtcat ggagagttca atctgaaaga aaacgctgaa
     1681 cttactgtta gtactattct ggcagataat ctcagccatc ataatataaa tagctgggac
     1741 ggaaaatccc taacaaaatc aggggaggga actctcattt tggcggaaaa aaatacctac
     1801 tctggtttca ccaacatcaa tgcaggcatt ctaaaaatgg ggacagttga agctatgaca
     1861 cgtaccgctg gtgttattgt taataaaggt gctaccttga atttttcagg catgaaccaa
     1921 actgttaaca ctttattaaa tagtgggact gtgctaatca ataatattaa tgcccctttt
     1981 ttgcctgacc ccgtcattgt cacaggtaac atgactctgg agaaaaacgg tcatgttatt
     2041 ctcaataata gttcgtcaaa tgtcggtcag acctatgttc agaaaggtaa ttggcatgga
     2101 aagggcggaa tattatcttt gggcgcggtt ctcggcaatg acaacagtaa aactgaccgg
     2161 ctggaaattg caggccatgc gtctggtatt acctatgttg cagtgacaaa tgagggaggc
     2221 tctggagata aaactcttga aggtgttcaa attatttcga cagattcttc tgataagaat
     2281 gcttttattc agaaaggccg tattgttgct ggtagttatg actatcgcct gaaacagggc
     2341 actgtatctg gactgaatac caataagtgg tatctaacta gtcagatgga taatcaagaa
     2401 tcaaaacaga tgagcaatca agagtctact caaatgagta gtcgccgagc tagttcacag
     2461 cttgtatctt cacttaattt gggtgaaggt agtattcaca catggcgccc tgaagctggc
     2521 agttatattg ctaacctgat agcaatgaac acgatgttta gtccttctct ctatgaccga
     2581 cacggtagca ctattgttga tcctactaca ggtcagctca gcgaaaccac catgtggatt
     2641 cgtactgttg gtggacataa tgagcataat ttagctgata gacaattaaa aaccacagct
     2701 aacaggatgg tttatcagat tggtggagat attttgaaga caaacttcac tgatcatgat
     2761 ggcttgcatg tgggtattat gggagcttat ggatatcagg atagcaaaac tcataataag
     2821 tatactagtt atagttcacg aggaactgtg agcggttata ctgccggttt gtacagttct
     2881 tggtttcagg atgaaaaaga acgaacaggt ctatatatgg atgcttggtt gcagtacagt
     2941 tggtttaata atacagtcaa aggagatggg ttaactggtg agaaatattc cagcaaagga
     3001 ataacaggag ctttggaagc tggctatatc tacccaacca tacgctggac tgctcataat
     3061 aatattgaca acgcattgta tctcaatcca caagtccaga taactaggca tggggtaaaa
     3121 gcaaacgact atattgaaca caatggcact atggtcacat cctctggggg caataatatt
     3181 caagcaaaat tgggattgcg tacatcctta attagtcaga gttgtatcga taaggagact
     3241 cttcgtaagt tcgaaccatt tttggaagtg aattggaaat ggagctcaaa gcaatatggt
     3301 gtaattatga atggcatgtc aaatcaccag ataggcaacc gtaatgtgat tgaactcaaa
     3361 actggtgtgg gggggcgtct tgcagataac ctaagcatct ggggaaacgt atctcagcaa
     3421 ttgggtaata acagttacag agacacccaa ggtattttgg gtgtgaaata taccttctga
     3481 taataaaaat gcagggcgtg ttgatgtcct gcatttctct gggacccacc gtgtaaaagc
     3541 tcggtgggtt tttgtgcaca tgatggtatg cctgcgatca atgccaaacg tgtttaccag
     3601 atcatgcgcc agaatgcgct gttgcttggc gttacacagg aattgcatta gaaagcgatg
     3661 aacgaatgct accgccaaat attcgtggtt atgcgcctca gattactggt attgcagaaa
     3721 ctaatgcacg tgttgttgta tctcaacaag gtcgagtaat atatgactct actgtaccag
     3781 ctggaacttt ttctattcag gacttgagta gttctgtacg aggaatattg gatgttgaaa
     3841 tttttgagca aaatggtaaa agaaaacatt tccaagtaga aatgtgcagg tgtgcctttc
     3901 ttatccagac ttggtcagag taggtataaa ttagtaacag gtctacctaa aacaaataat
     3961 aaagcaactg gtgatgcatt tttttcggtg aagatcctac ggggtcctga gcccggtgaa
     4021 gtggcgaggc aaatcacatg gggaagcgta cagcctga
//
LOCUS       DAZPFP010000242         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPFP010000242 DAZPFP010000000
VERSION     DAZPFP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.247"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHUR4_003785"
     CDS             complement(126..284)
                     /locus_tag="ACHUR4_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1457156.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHUR4_003786"
     CDS             complement(886..1077)
                     /locus_tag="ACHUR4_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1457157.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHUR4_003787"
     CDS             1142..1465
                     /locus_tag="ACHUR4_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1457158.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHUR4_003788"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHUR4_003788"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHUR4_003789"
     CDS             2143..2673
                     /locus_tag="ACHUR4_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1457159.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHUR4_003790"
     CDS             2680..2913
                     /locus_tag="ACHUR4_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1457160.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHUR4_003791"
     CDS             complement(3459..3851)
                     /locus_tag="ACHUR4_003791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457161.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZPFP010000243         4028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPFP010000243 DAZPFP010000000
VERSION     DAZPFP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.248"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            377..580
                     /locus_tag="ACHUR4_003792"
                     /pseudo
     CDS             377..580
                     /locus_tag="ACHUR4_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            599..3985
                     /locus_tag="ACHUR4_003793"
     CDS             599..3985
                     /locus_tag="ACHUR4_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1457162.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
BASE COUNT          991 a          925 c         1264 g          848 t
ORIGIN      
        1 ttgttgttaa taaaaagctt atagatttat agttgataat taaaacgcat ccgatatttc
       61 agctttttct atgcaagata caattttata aggtgtccat ttttatatgg aggcggtagc
      121 gtgtctgtta ctagtggttt tatttgcata gtgaatatat tgggattgtt aaatacataa
      181 ctatgtatcc gctctggtcg ttgtaaatat tgctaattgc ttaatattat tagtgattat
      241 gactggaaag ataaagtgtg ctgtaaagcg gtgatgggta ttacattcat gattaaaaaa
      301 acagaacctg tcaataaata attcctttat taaaacggac tatttacatt atgacggaat
      361 attatctcaa cgaggcggtt tggaaataca gagccagttt ctccggggta acgagcaggt
      421 gtaccagtgt caggtggcat ttgtattgcc gggtgagcgg gtcatgatgg ccttcaccta
      481 cgccagaacc acaccgctga ccccggcaga tatgacgcgc tgggctgaaa ttaaaaagaa
      541 tctgcgcttc aggatgcggc aggaagtcag gacgaattaa tcatacaggg aacgggacat
      601 ggaagaggca ttctgggcgg caagacaggg tgatgccctg ctgcatacgt cgtttatggc
      661 agatgtgctg ggggcggtgc tggaggtggc cgcaaatgtc gttattgatg ccctcattgt
      721 cggcacaacc tcgttactgg cgacgtcagt ggggatcacg atggggtgca cggccgtcgt
      781 gctgaccggt tttgtggcgg gtgtggcgat ggtgtacacc ggcgtgacag aaaaggtcag
      841 cgaagcctgt tcagccctcg caaatatgct tttcccgcct cagattgaag gttacatcct
      901 caccggctct ggtgacacat ggatcaacag caaacctgcc gcgcgggcag cggcgactgc
      961 ggcatcgcgg caggacatag aggcgcagga agcgcaggcg aaggcagaac aggaagaagc
     1021 gcagcgtcag gaggatgcca gaacgttccg ggatgtggcc ggggaatatc tggaaatggc
     1081 gggggccata gccctggcgg ttaacccggt gacagggcct gtgctgatgt cccgggctct
     1141 gcacgaccag ctcagtacgg aagaagggcg ttctgaaatg gtggatggcg tgacacattt
     1201 tgtgtcggaa ctatggcagc ccactgtggc cagtgcggcc ccgggatcca ccccgacccc
     1261 tgacgataaa atcgactgcc ataaacatcc ttcctcactg acgcagtttc tcgcacaaaa
     1321 aaaagccgcc tttctggatg cccccctggg aacggccctt aacctgctga atccgggcgg
     1381 tatgctggaa accgcctttc agggagtgaa tgcggttatt ggcagcatca gtaatctgtt
     1441 caaaggcgat gacgagccgc ccgcagcgga atatatcgcg gaaggcgcgc gggatgtgcg
     1501 tatcaacagc cagccggcga cacgcagcgg ggcacgctgt acctgcgagg ccagggtggt
     1561 gaatgagccg gggaacgggg cgtttgtgtc accggatgtg cgcattggcg gtccgttact
     1621 ggtggtcagg gatatcagaa gcggcaggtc gcagataacc ctggtggcaa ccgtggcgct
     1681 gatgtttctt cgcccgggca aaatgctttc taaaatcgcc tgttttgctg ccagtgtcgg
     1741 gatgagcatg ctgacgcaga aggccaccag tgcccttccg catccggtca atgccgccac
     1801 cggggccaaa tacctggccg atgacgatga cttcgatttc agtctgccgg gccactttcc
     1861 gctggactgg cagcgcgtgt acagcagtcg tgacgaacgt acggaaggaa tgtttggtca
     1921 gggctggagt gtaatgtatg aagtcagcct cgtgtgcaca ccgggtagcg cagacgaaaa
     1981 ctgcatgacc tttgtgtcgg gcatggggcg acgcttggat atggaagccg tactgccggg
     2041 gggcgggttt tacagccccg gtgaagggct ggcggtaagg cgtggtgagc aggggcactg
     2101 gcttatcagc agcgatgacg ggcagtgttt tctgtttgag gctgacccgc accacccgca
     2161 gcgccagcgg ctgaaaatgc tgggcgaccg taacagcaac tgtctgaatc tgtactacga
     2221 cgaccggggc cgcattgtgg aaatcagcgg cgaacagcag cgtccgtgca tccgtctgta
     2281 ttacgaactg gcggcgcatc cccggcgggt gacacagatt tatcagcatt tccctgagac
     2341 tgcaccttta ctgctgcgtc gttacagcta tgacgaggcc ggccacctga acggcgtgta
     2401 cgacagcacg gggcacctgc tgcgtgagtt tgcttacgac gaaaaccact gcatgacgct
     2461 gcaccgccag ccgggcggcg aagggtatta ttaccagtgg ggctggtatg aaggtccgga
     2521 tgacgcggca tggcgggtga cggggcacca cacggacagc ggtgaacagt accggctggc
     2581 gtgggacctg gcgcagcgca ggctgtgcgt gacggacgga ctggggcgca cacgttatca
     2641 ccagtgggat gcgcagaatc aggtgacggc gtaccaggac gaagccggac aggtgacgac
     2701 attccgctgg agcgacgaag aacgactgtt gctggggatg accgaccctc agggcggcaa
     2761 atggcgctat gtgtatgacc tgcaggggca tatcacggag acgcacgacc cgctggggcg
     2821 ggtggagcag gcgcagtggc acccggtgtg gcaccagccg gaaacggagg tggacgcggc
     2881 gggaaacctg ctggggaggc gcgcggggga aagggccacg gcagaaaaca gcgtggtgcc
     2941 gttcaaccgg ctgatgtctt accgtggtgt gcactaccgt tatgacgaat ttggccgtgc
     3001 ggtggaaaag gagggccgga gcggaacgca gagttaccgt tatgatgcgg aacaccggat
     3061 ggtggaagtg acgacagcgc gggggacata ccggtatgtg tacgatgcac tggggaggcg
     3121 gacggaaaaa cagcacatca gccctgacgg taagccgtac aaccggacaa aatttttatg
     3181 ggacggaatg cggctggcgc aggagagcag gccggaggga acaggcagcc tgtacatcta
     3241 cagggaccag gggagttatg aaccgctggc gcgggtggat aaggcgggca aagagggccc
     3301 gaaccggata ctgtattttc acacggatgt gaacggcgcg ccggaagaga tgacggacag
     3361 cgacgggaaa atcgtgtggg agacggggta tcaggtctgg ggtaacacga tacaggagaa
     3421 ggaccacggc ggggtggagc agaacctgcg ctaccaggga cagtacctcg acagggaaac
     3481 ggggctgcac tacaatctgc acaggtacta tgatccggat gtcgggcggt tcatggtaac
     3541 tgacccgata gggttgagag gcggtttaaa tctctacagt tatgcaccta atcctctaaa
     3601 atatgctgat ccacttggat taactccatg tgctgttagt aaccaaaaag cgaatcgttt
     3661 attagatagt agtgaaacca aagttactgt taggtctaga agtgacgctg aacaattgtt
     3721 tatggataga tatttaggtc ataattataa aaatatgaca ggtgagtcag gccccagcac
     3781 taagaactta atggaatatc ttactgaaaa taaaacaaaa gcaggttcat atcattggga
     3841 tgatataaaa gatccttctg taacaaaacc ttcatataga gtgtcaggtc atgggccagg
     3901 aaatcctgat ggtgatttac cacatttaca agttcatcaa catggtggaa gtgttagaca
     3961 tatatttttc ccgtgggaga cataataatg aacaatctca atacattatt aagtggaaag
     4021 gttttgct
//
LOCUS       DAZPFP010000244         3931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPFP010000244 DAZPFP010000000
VERSION     DAZPFP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.249"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHUR4_003794"
     CDS             72..302
                     /locus_tag="ACHUR4_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457163.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHUR4_003795"
     CDS             280..780
                     /locus_tag="ACHUR4_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1457164.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHUR4_003796"
     CDS             780..2198
                     /locus_tag="ACHUR4_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1457165.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2198..3046
                     /locus_tag="ACHUR4_003797"
     CDS             2198..3046
                     /locus_tag="ACHUR4_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1457166.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3046..3501
                     /locus_tag="ACHUR4_003798"
     CDS             3046..3501
                     /locus_tag="ACHUR4_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1457167.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3504..>3931
                     /locus_tag="ACHUR4_003799"
     CDS             3504..>3931
                     /locus_tag="ACHUR4_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1457168.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT         1034 a          957 c         1013 g          927 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca atatcttctc
     2281 gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg tggctaacaa
     2341 agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg agcaggatgt
     2401 gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga
     2461 ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa tagctcagaa
     2521 tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc aggtttcaga
     2581 ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt cagaggttgt
     2641 ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta gcctgctgaa
     2701 aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta acaacaagat
     2761 ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc
     2821 cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc ctgacacact
     2881 ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta cgttcttcag
     2941 cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc agtcgaatgg
     3001 tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3061 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3121 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3181 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     3241 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     3301 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     3361 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     3421 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     3481 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgcgcg
     3541 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     3601 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     3661 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     3721 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     3781 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     3841 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     3901 cttggttatc aaatcagatg agcaattaca a
//
LOCUS       DAZPFP010000245         3904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPFP010000245 DAZPFP010000000
VERSION     DAZPFP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.250"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUR4_003800"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUR4_003800"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1457169.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUR4_003801"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUR4_003801"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1457170.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          989 a         1036 c          981 g          898 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgc
//
LOCUS       DAZPFP010000246         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPFP010000246 DAZPFP010000000
VERSION     DAZPFP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.251"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHUR4_003802"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHUR4_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHUR4_003803"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHUR4_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHUR4_003804"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHUR4_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHUR4_003805"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHUR4_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZPFP010000247         3803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPFP010000247 DAZPFP010000000
VERSION     DAZPFP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.252"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..746
                     /locus_tag="ACHUR4_003806"
     CDS             <1..746
                     /locus_tag="ACHUR4_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457171.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUR4_003807"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUR4_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1457172.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..>3803
                     /gene="safA"
                     /locus_tag="ACHUR4_003808"
     CDS             3607..>3803
                     /gene="safA"
                     /locus_tag="ACHUR4_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1457173.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT         1045 a          851 c          901 g         1006 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctg
//
LOCUS       DAZPFP010000248         3785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPFP010000248 DAZPFP010000000
VERSION     DAZPFP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.253"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            2..256
                     /gene="yoeB"
                     /locus_tag="ACHUR4_003809"
     CDS             2..256
                     /gene="yoeB"
                     /locus_tag="ACHUR4_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1457174.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            339..1163
                     /locus_tag="ACHUR4_003810"
     CDS             339..1163
                     /locus_tag="ACHUR4_003810"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1457175.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1209..2192
                     /locus_tag="ACHUR4_003811"
     CDS             1209..2192
                     /locus_tag="ACHUR4_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1457176.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUR4_003812"
     CDS             2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUR4_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1457177.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUR4_003813"
     CDS             2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUR4_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1457178.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          890 a          896 c          997 g         1002 t
ORIGIN      
        1 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaaacaga
       61 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      121 aggtaagggg aaaccagaac ccctgaaaca taatttgtca ggtttctggt cccgacgcat
      181 tacagaggag caccgtctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      241 tcgttatcat tattgaacag attcattcat agaatgaatt gtcagttttg atacgctagc
      301 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      361 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      421 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
      481 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
      541 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
      601 atttacaagc ggtacaagag ttagtggata gcgcgctggc ccatcgtatt ccccgaatta
      661 tttttaccag ctcaacatct gtctatggcg acgcgcaagg cacggttaaa gaaactaccc
      721 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
      781 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
      841 atccgggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
      901 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
      961 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1021 cccgtttact ggggctgcaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1081 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1141 cgctggtaat gccgttggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1201 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgccata gaaaaagagg
     1261 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1321 ttcacaagct ggaaagcgac ctaaatattc aattgcttga tcgtagcgga caccgcgcta
     1381 aattcacccg caccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1441 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     1501 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     1561 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     1621 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     1681 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     1741 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     1801 tggtggggga tactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     1861 aagccattac cgttttcgat tttaaaacca aactggagct gcaaattagc ggccttggct
     1921 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     1981 agaaagtggt cgcccaaact ctctttgaac ctgtctggat tggctggaat gaacagaccg
     2041 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2101 tttatgcaaa atctgatgac ggaaaatcag ccattcaaag aaaaattatt atgacaagcc
     2161 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgat
     2221 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2281 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2341 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2401 aaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     2461 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     2521 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     2581 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     2641 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     2701 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     2761 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg cgctggtggc
     2821 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacgtca acactgtaat
     2881 cgtcgtgttg caggtagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     2941 tgaaggtgaa ggcgctggta cgctggcgag cacgcgtccg ttctggtctg gcgatgcgca
     3001 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gctttgacgg
     3061 catcagcaat ctgtcggaag aaaccaaaga tgcagagcgc gtgatcccgc gtgcgatttt
     3121 cctgaccgcg ctgattggcg gcatgatctt catctttgca acttacttcc tgcagctgta
     3181 cttcccggat atctctcgct ttaaagatcc ggatgcgtca cagcctgaaa tcatgctgta
     3241 cgttgcgggt aaagcgttcc aggttggcgc gctgatcttc tccaccatta ccgtactggc
     3301 gtccggtatg gcggcgcacg caggcgtagc gcgtctgatg tacgtaatgg gtcgtgacgg
     3361 cgtgttcccg aaaagcttct tcggttatgt acacccgaaa tggcgtactc cggcgatgaa
     3421 catcatcctg gttggcgcga ttgccctgct ggcaatcaac ttcgacctgg taatggcaac
     3481 ggcgctgatt aactttggtg cgctggtggc gttcaccttt gttaacctgt cggtcatctc
     3541 gcagttctgg atccgtgaga agcgtaacaa gacgctgaaa gatcacttcc agtatctgtt
     3601 cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg gttaacctgg aagaaagctc
     3661 aatggttctg ggtctgatct gggctgctat cggtctgatt tacctggctt gcgtcaccaa
     3721 gagcttccgt aatccggttc cgcagtacga agacgtagca taatcatctt ttctaataat
     3781 aaacc
//
LOCUS       DAZPFP010000249         3770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPFP010000249 DAZPFP010000000
VERSION     DAZPFP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.254"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            62..337
                     /gene="ypdI"
                     /locus_tag="ACHUR4_003814"
     CDS             62..337
                     /gene="ypdI"
                     /locus_tag="ACHUR4_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1457179.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(414..656)
                     /locus_tag="ACHUR4_003815"
     CDS             complement(414..656)
                     /locus_tag="ACHUR4_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1457180.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1009..1929
                     /gene="lpxP"
                     /locus_tag="ACHUR4_003816"
     CDS             1009..1929
                     /gene="lpxP"
                     /locus_tag="ACHUR4_003816"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1457181.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2284..2355
                     /gene="ypdK"
                     /locus_tag="ACHUR4_003817"
     CDS             2284..2355
                     /gene="ypdK"
                     /locus_tag="ACHUR4_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1457182.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2420..3658)
                     /gene="alaC"
                     /locus_tag="ACHUR4_003818"
     CDS             complement(2420..3658)
                     /gene="alaC"
                     /locus_tag="ACHUR4_003818"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1457183.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT          974 a          959 c          879 g          958 t
ORIGIN      
        1 tatgtaaata cagccataac atcgtatggt tctcagtgaa ccattttcag gataactttc
       61 tatgaaagta aacttaatac ttttcagctt atttttattg gtctctatta tggcatgcaa
      121 tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag agactgaaaa
      181 aacatcagcg atgacgacca cacactctac aaaacttcag ccatcacagg cgattttgtt
      241 taagatgaga gaagatgtgc caccattaaa cctcacagaa gaaatcacgc ctacttatcc
      301 gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg gcaggtgaac
      361 aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac ccactatcgc
      421 ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag aaacataaat
      481 atccccgtca ccagttcatt accgccagtt aaccacatgg tgaagtaata aatgagtgca
      541 gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag tacctgaccg
      601 atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata aatcatagct
      661 caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta agacaatcct
      721 taacctctgg caatttttgc taagcatcaa tgaaaacagc acgttaaatg caacgctatt
      781 gcgtgcggaa aattttttac tttgcacgat taataatcag tagctgaaag cagtcagcca
      841 gtgataaagt agagccaccg cattcatgct cctcgtggtt atgtcctgac tagtctttac
      901 actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt accgctgtcg
      961 acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat gtttccacaa
     1021 tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt tgggcttggt
     1081 gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg cacgcgtatt
     1141 ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa aaacctggaa
     1201 ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga aaactttcgt
     1261 tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga cagtcgcgta
     1321 cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat gcaaaatcgc
     1381 ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg cgtgatggga
     1441 ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat ggaatgggtg
     1501 cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc
     1561 attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca ggattatggt
     1621 cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac aaccaatggc
     1681 acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat ggtaagaaaa
     1741 gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta cccgacagat
     1801 gaaaatcaag ccgctgctta tatgaacaag attatcgaga aagagatcat gcgcgcaccg
     1861 gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgttg
     1921 tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat aacctcatga
     1981 tttttatagc ttttttctta agttttttcc ttcgctgaat atctaattat taccgtcaga
     2041 aattaaactg tcgccgatat gagacacttg taagttacta aaagtaaata agaaaatgcc
     2101 tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt tagctcagaa
     2161 ttctggggtg atgcccacgg taaaatacag ggcttttaag gtcgctttgc ccggtgtcaa
     2221 ctcactgtat caggtgtaat gaagtcattc aggcgtaaca gtaattacgc ggagagatgt
     2281 aaagtgaaat atttctttat gggcatttct tttatggtca tcgtttgggc cggtactttt
     2341 gccctgatga tctaaaagca aactgtgcaa tgaaaaacag gagccatcag gctcctgttt
     2401 gcatttctga cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg ctgacgggta
     2461 gtaaaccgtc ggcacggaac atcgctttaa tccctctgat ggcctgacga atacggtcgt
     2521 ggttttcaat cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag ccaatccccg
     2581 gcgagacaca aaccttcgct tcgttaagga gcttcttggc aaattccagc gatcccatgg
     2641 ccgcatatgg ttccgggatt ttcgcccaga catacatcga agccttcggc atttcgacca
     2701 tccagcccgc ttcatgcagc cctttaacca atacatcacg acggcgtttg tactgttcgg
     2761 caatgtcgcg cacgcactgt tgatcgccct cgagtgccgc aatcgccgcc acctgcaacg
     2821 gcgtaaaagt gccgtaatcg tgatagcttt tgattcgcgc cagggcactg accagcgttt
     2881 tgttgccgac cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa
     2941 agaactcgac cgccacatcg cgtgcacccg gcacctgcat aattgacggc gctttccagc
     3001 catcgtagac gatatcggca taggccaggt catggaccac cagcacatcg tagcatttcg
     3061 ccagcgccac aaccttttcg aagaactcca gttccacgca ttgcgcggtt gggttcgacg
     3121 ggaagccgag gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt
     3181 cgttgaagaa atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca
     3241 ccgcgccata aatatggatc gggtaacttg gattcggcac cagcaccgtg tcaccatgat
     3301 ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt
     3361 ctgattccgg gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac
     3421 gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc tgggccacag
     3481 tacataattt ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat
     3541 cgataatatc ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa
     3601 cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa
     3661 tttcctcaga ttaacgttag cgcccggacc gtccgagcga cgctgccaca atggtggctg
     3721 ttttgaaaat agcctgatta atttctgtct gtccagcgtt ttagaacatt
//
LOCUS       DAZPFP010000250         3758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPFP010000250 DAZPFP010000000
VERSION     DAZPFP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.255"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUR4_003819"
     CDS             complement(139..3654)
                     /locus_tag="ACHUR4_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1457184.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          949 a          968 c          784 g         1057 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgttt
//
LOCUS       DAZPFP010000251         3672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPFP010000251 DAZPFP010000000
VERSION     DAZPFP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.256"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHUR4_003820"
     CDS             <1..220
                     /locus_tag="ACHUR4_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457185.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHUR4_003821"
     CDS             complement(392..721)
                     /locus_tag="ACHUR4_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1457186.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHUR4_003822"
     CDS             complement(726..911)
                     /locus_tag="ACHUR4_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1457187.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHUR4_003823"
     CDS             complement(908..3547)
                     /locus_tag="ACHUR4_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1457188.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          902 a          978 c          834 g          958 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cg
//
LOCUS       DAZPFP010000252         3634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPFP010000252 DAZPFP010000000
VERSION     DAZPFP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.257"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(5..49)
                     /locus_tag="ACHUR4_003824"
     CDS             complement(5..49)
                     /locus_tag="ACHUR4_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457189.1"
                     /translation="MKRNDKILHYEGLN"
     gene            168..1892
                     /gene="ilvI"
                     /locus_tag="ACHUR4_003825"
     CDS             168..1892
                     /gene="ilvI"
                     /locus_tag="ACHUR4_003825"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1457190.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1895..2386
                     /gene="ilvN"
                     /locus_tag="ACHUR4_003826"
     CDS             1895..2386
                     /gene="ilvN"
                     /locus_tag="ACHUR4_003826"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1457191.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2398..2484
                     /locus_tag="ACHUR4_003827"
     CDS             2398..2484
                     /locus_tag="ACHUR4_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1457192.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2566..3570
                     /gene="cra"
                     /locus_tag="ACHUR4_003828"
     CDS             2566..3570
                     /gene="cra"
                     /locus_tag="ACHUR4_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1457193.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          844 a          875 c         1008 g          907 t
ORIGIN      
        1 attatcaatt taatccttcg taatggagga ttttatcgtt tcttttcacc tttcctcctg
       61 tttattctta ttaccctgcg tttatgtctc tggctgccaa ttgcttaagc aagatcggac
      121 ggttaatgtg ttttacacat tttttccgtc aaacagtgag gcaggccatg gagatgttgt
      181 ctggagccga gatggtcgtc cgatcgctta tcgatcaggg cgttaaacaa gtattcggtt
      241 atcccggagg cgcagtcctt gatatttacg atgcattgca taccgtgggt ggtattgatc
      301 atgtattagt tcgtcatgag caggcggcgg tgcatatggc cgatggcctg gcgcgtgcga
      361 ccggggaagt cggcgtcgtg ctggtaacgt cgggtccaag ggcgaccaat gcgattaccg
      421 gcatcgccac cgcttatatg gattccattc cattagttgt cctttccggg caggtagcga
      481 cctcgttgat aggttacgat gcctttcagg agtgcgacat ggtggggatt tcgcgaccgg
      541 tggttaaaca cagttttctg gttaagcaaa cggaagacat tccgcaggtg ctgaaaaagg
      601 ctttctggct ggcggcaagt ggtcgcccag gaccagtggt cgttgattta ccgaaagata
      661 ttcttaatcc ggcgaacaaa ttaccctatg tctgccctga gtcggtcagt atgcgttctt
      721 acaatcccac tactaccgga cataaagggc aaattaagcg tgctctgcaa acgctggtag
      781 cggcaaaaaa accggttgtc tacgtaggcg gtggggcaat catggcgggc tgccatcagc
      841 agttgaaaga aacggtggag gcgttgaatc tgcccgttgt ttcctcattg atgggaatgg
      901 gggcgtttcc ggcaacgcat cgtcaggcac tgggcatgct gggaatgcac ggtacctacg
      961 aagccaatat gacgatgcat aacgcggatg tgattttcgc cgtcggggta cgatttgatg
     1021 accgaacgac gaacaatctg gcaaagtact gcccaaatgc cactgttctg catatcgata
     1081 ttgatcctac ttccatttct aaaaccgtga ctgcggatat cccgattgtg ggggatgctc
     1141 gccaggtcct cgaacaaatg cttgaactcc tgtcgcaaga atccgcccat caaccgctgg
     1201 atgagatccg cgactggtgg cagcagattg aacagtggcg cgctcttcag tgcctgaaat
     1261 atgacactca cagtgaaaag attaaaccgc aggcggtgat cgagactctt tggcggttga
     1321 ctaatgggga cgcttacgtg acgtccgatg tcgggcagca ccagatgttt gctgcgcttt
     1381 attatccatt cgacaaaccg cgtcgctgga tcaattccgg cggtctcggc acgatgggtt
     1441 ttggtttacc tgcggcactg ggcgtcaaaa tggcgttgcc agaagaaacc gtggtttgcg
     1501 tcactggcga cggcagtatt cagatgaaca tccaggaact gtctaccgcg ttgcaatacg
     1561 agttgcccgt actggtggtg aatctcaata accgctatct ggggatggtg aaacagtggc
     1621 aggacatgat ctattccggc cgtcattcac aatcttatat gcaatcacta cccgatttcg
     1681 tccgtctggc ggaagcctat ggtcacgtcg ggatccagat ctctcatccg catgagctgg
     1741 aaagcaaact tagcgaggcg ctggaacagg tgcgcaataa tcgcctggtg tttgttgatg
     1801 ttaccgtcga tggcagtgag cacgtctacc cgatgcagat tcgcgggggc ggaatggatg
     1861 aaatgtggtt aagcaaaacg gagagaacct gattatgcgc cggatattat cagtcttact
     1921 cgaaaatgaa tcaggcgcgt tatcccgcgt gattggcctt ttttcccagc gaggctacaa
     1981 cattgaaagc ctgaccgttg cgccaaccga cgatccgaca ttatcgcgta tgaccatcca
     2041 gaccgtgggc gatgaaaaag tacttgagca gatcgaaaag caattacaca agctggtcga
     2101 tgtcttgcgc gtgagtgagt tggggcaggg cgcgcatgtt gagcgggaaa tcatgctggt
     2161 gaaaattcag gccagcggtt acgggcgtga cgaagtgaaa cgtaatacgg aaatattccg
     2221 tgggcaaatt atcgatgtca ccccctcgct ttataccgtt caattagcag gcaccagcga
     2281 taagcttgat gcatttttag catcgattcg cgatgtggcg aaaattgtgg aagttgctcg
     2341 ctctggtgtg gtcggacttt cgcgcggcga taaaataatg cgttgagaat gatctcagtg
     2401 cgcaatttgc agcccaacat gtcacgttgg gctttttttg cgaaatcagt gggaacctgg
     2461 aataaaagca gttgccgcag ttaattttct gcgcttagat gttaatgaat ttaacccata
     2521 ccagtacaat ggctatggtt tttacatttt acgcaagggg caattgtgaa actggatgaa
     2581 atcgctcggc tggcgggagt gtcgcggacc actgcaagct atgttattaa cggcaaagcg
     2641 aagcaatacc gtgtgagcga caaaaccgtt gaaaaagtca tggctgtggt gcgtgagcac
     2701 aattaccacc cgaacgccgt ggcagctggg cttcgtgctg gacgcacacg ttctattggt
     2761 cttgtgatcc ccgatctgga gaacaccagc tatacccgca tcgctaacta tcttgaacgc
     2821 caggcgcggc aacggggtta tcaactgctg attgcctgct cagaagatca gccagacaac
     2881 gaaatgcgct gcattgagca ccttttacag cgtcaggttg atgccattat tgtttcgacg
     2941 tcgttgccac ctgagcatcc tttttatcaa cgctgggcta acgacccgtt cccgattgtc
     3001 gcgctggacc gcgccctcga tcgtgaacat ttcaccagcg tggttggtgc cgatcaggat
     3061 gatgccgaaa tgctggcgga agagttacgt aagtttcccg ccgagacggt gctttatctt
     3121 ggcgcgctgc cggagctttc tgtcagcttc ctgcgtgaac aaggtttccg tactgcttgg
     3181 aaagatgatc cgcgcgaagt gcatttcctg tatgccaaca gctatgagcg ggaggcggct
     3241 gcccagttat tcgaaaaatg gctggaaacg catccgatgc cgcaggcgct gttcacaacg
     3301 tcgtttgcgt tgttgcaagg agtgatggat gtcacgctgc gtcgcgacgg caaactgcct
     3361 tctgacctgg caattgccac ctttggcgat aacgaactgc tcgacttctt acagtgtccg
     3421 gtgctggcag tggctcaacg tcaccgcgat gtcgcagagc gtgtgctgga gattgtcctg
     3481 gcaagcctgg acgaaccgcg taagccaaaa cctggtttaa cgcgcattaa acgtaatctc
     3541 tatcgccgcg gcgtgctcag ccgtagctaa tccgcgaaca aaaatacgcg ccaggtgaat
     3601 ttccctctgg cgcgtagagt acgggactgg acat
//
LOCUS       DAZPFP010000253         3596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPFP010000253 DAZPFP010000000
VERSION     DAZPFP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.258"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..444
                     /locus_tag="ACHUR4_003829"
     CDS             <1..444
                     /locus_tag="ACHUR4_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1457194.1"
                     /translation="ASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDVSPGINVGYIFA
                     DADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTARYGKERLVKLSA
                     MDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
     gene            445..1863
                     /locus_tag="ACHUR4_003830"
     CDS             445..1863
                     /locus_tag="ACHUR4_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1457195.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            1863..2711
                     /locus_tag="ACHUR4_003831"
     CDS             1863..2711
                     /locus_tag="ACHUR4_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1457196.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            2711..3166
                     /locus_tag="ACHUR4_003832"
     CDS             2711..3166
                     /locus_tag="ACHUR4_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1457197.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3169..>3596
                     /locus_tag="ACHUR4_003833"
     CDS             3169..>3596
                     /locus_tag="ACHUR4_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1457198.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT          935 a          890 c          933 g          838 t
ORIGIN      
        1 gcatcaaatg ccacgttaac cgatgtagaa ccgcagtcta tggaagacgg cgtcaacgac
       61 cttgaaatga tgatggctga atggcttggc ggtgatgtgt cacctggtat caacgttggc
      121 tacattttcg ctgatgcaga tgtcgctcca gaaccgggcg atgagcacgg tttatcaaat
      181 aacgctatca atgccgtcat tttcaacctt gcctgccgca ttgctccaga ttatgcgctg
      241 gaagcgccag caaaacttat aaccactgcc agatacggga aagagcgact cgtcaaactg
      301 tctgcaatgg acagagcaaa agccgctaaa tgtaagtccg gttatccaaa ccgtatgcct
      361 gttggtagcg gtaaccagtt ggcgaagtgg aatggttgga attacttcca ccggaaggaa
      421 ccttgcgata acgggggcga ataaatgccg attcagcaac ttccgcttat gaaaggtgtc
      481 ggcaaagatt tccgaaacgc cgactacatc gactatctgc cagtgaatat gctggctaca
      541 cccaaagaaa tactcaacag cagcggatat cttcgctcat tcccgggcat tgccaaacgt
      601 tctgatgtga atggtgtatc gcgcggtgtc gagtacaaca tggcgcagaa tgctgtttat
      661 cgcgtttgcg gtggcaagtt gtataaggga gaaagcgagg tcggtgatgt tgccggaagt
      721 ggtcgcgtat caatggcgca tggtcggaca tcacaggcgg taggcgttaa tggtcaactg
      781 gtcgagtatc gctatgatgg cacggttaaa accgtctcaa actggcctac agacagtgga
      841 ttcactcagt atgagttagg ttcagttcgc gacattacgc gcttacgtgg gcgttatgcg
      901 tggtcaaaag acggcactga ttcatggttt atcactgacc ttgaagatga atcgcatcct
      961 gaccgctaca gtgcacaata tcgtgccgag tctcagccgg acggcatcat cggcataggt
     1021 acatggcgag acttcatcgt ctgctttggc tcatcgacga ttgaatattt ttccctgaca
     1081 ggtgcaacca ccgttggtgc cgctttgtat gtcgcacagc catcgctgat ggtgcagaaa
     1141 ggtattgccg ggacttactg caaaacacca ttcgctgatt cttatgcgtt catcagcaat
     1201 ccggcaacag gtgcgccgtc tgtatacatc atcggctccg gtcaggtgtc accaatcgcc
     1261 agcgcgagca ttgagaaaat cctccgctcc tacactgctg atgaactggc tgatggcgtg
     1321 atggaatcgc tgcggtttga tgcgcatgag ttgctgatta tccaccttcc gcgccatgta
     1381 ctggtgtacg acgcatcttc aagcgccaat ggtccgcaat ggtgtgtgct gaaaacaggc
     1441 ctgtatgacg atgtgtaccg cgctatcgac ttcatttacg aaggcaatca aataacgtgc
     1501 ggcgataagc tggagtccgt gaccgggaaa ttgcaattcg acatcagcag ccagtacgac
     1561 aagcaacagg aacacctgct gtttactcca ctgttcaaag cggataacgc cagagtgttc
     1621 gaccttgagg ttgaatcgtc aactggcgtt gcgcagtatg ctgaccgcct ttttctctct
     1681 gcaaccactg acggcatcaa ttacgggtgt gagcagatga ttgagcagaa tgaaccgttc
     1741 gtttacgaca aacgcgtttt gtggaagcga gtagggcgca tcaggaaaaa tgtcggcttc
     1801 aaattgcgcg ttatcacgaa gtcacctgtc actctgtctg gctgccagat aaggattgag
     1861 taatggcgga ttcgaatctc aatgtgccgg taatcattca ggctacacgg ctcgacacat
     1921 cagtccttcc acgcaatatc ttctcgcagt cgtatctgct ttacgttatc gcacagggca
     1981 ctgatgttgg taacgtggcc aacaaggcca acgaggccgg acagggcgct tatgacgcac
     2041 aagtcaggaa cgatgagcag gatgtgattc tcgctgacca tgagcagcga atttctgctg
     2101 cggaagcaac gcttgttaat catgaggagc gaatcagcca ggcagaatca actcttcagg
     2161 aacatgaaac gcgaatcgct cagaatgaaa gcgatattgc gtcgcttgat accagagttc
     2221 agtcgctgga atcgcaggtt tcagaccatg aaacgcgcat cgatgctctg gagtatgcca
     2281 ctactcgcaa gaagtcagag gttgtttact ctggcgtatc tgtaaccatc ccgacagcgc
     2341 cgaccaacct tgttagccta ctgaaaacgc tcacgccgtc atccggcacg ttggcaccat
     2401 tcttcgacac cgttaacaac aagatggttg tgttcaacga gaacaaaacc ttgttcttca
     2461 agctgtcgat cgtcgggacg tggcccagcg gaaccgccaa caggtcaatg cagctaacct
     2521 tttccggctc tgttcctgat acgctggtca gcagtcgtaa tgcggcgaca acaaccgaca
     2581 acatcctgtt agctacattc ttcagcgtgg ataaagacgg atttcttgcc acaaatggca
     2641 gtacgttaac cattcagtcg aatggtgcgg cgtttactgc cacaaccatc aagataatcg
     2701 cggagcagtg atgattcagt tcaaaccaac gcgaaacatc gacctgatag aagccgtggg
     2761 aaatcacccc gacattatcg ccgggagcaa caacggtgat ggatacgact acaagcctga
     2821 atgccgttac ttcgaggtga acgtgcacgg gcagttcggc ggcattgttt actatcagga
     2881 gattcagcct ttgacctttg attgccacgc catgtacctg ccagaggttc gtggattcag
     2941 caaggaaatc gggctggcgt tctggcgata cattctgact aacaccaccg ttcagtgcgt
     3001 cacatcgttc gctgcgcgca aattccgcca cggtcagatg tactgcgcaa tgattggcct
     3061 taagcgagta ggaaccatca agaaatactt caaaggcgtg gatgacgtga cattttacag
     3121 tgctacacgc gaagaactaa tcgacttcct gaatcacggg agatagccat gttatatgca
     3181 tttaagctgg gcagaaaact gcgcggcgag gaaccttatt gccctgaaaa aggcgggaaa
     3241 ggtggcagct ctgataaaag tgcaaagtat gcagcagaag ctcagaagta tgccgcagac
     3301 ctgcaaaatc agcagtggca gacgatcatg aaaaaccttg ctccgttcac gccgcttgcg
     3361 gagcagtatg ttaaccagct tcagaacctt tccagtttag aaggacaggg tcaggcactt
     3421 aatcagtatt acaactctca gcagtataaa gaccttgcag gtcaggctcg ttaccagagt
     3481 cttgctgctg cggaggctac gggaggactt ggttcgacag ccacaagcaa tcaactggct
     3541 acgattgctc cgactctcgg tcagtcgtgg ttatcaaacc agatgagcaa ttacaa
//
LOCUS       DAZPFP010000254         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPFP010000254 DAZPFP010000000
VERSION     DAZPFP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.259"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUR4_003834"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUR4_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1457199.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHUR4_003835"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHUR4_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1457200.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUR4_003836"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUR4_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1457201.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZPFP010000255         3527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPFP010000255 DAZPFP010000000
VERSION     DAZPFP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.260"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..615)
                     /locus_tag="ACHUR4_003837"
     CDS             complement(<1..615)
                     /locus_tag="ACHUR4_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1457202.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKH"
     gene            861..1622
                     /locus_tag="ACHUR4_003838"
                     /pseudo
     CDS             861..1622
                     /locus_tag="ACHUR4_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1729..2241)
                     /locus_tag="ACHUR4_003839"
     CDS             complement(1729..2241)
                     /locus_tag="ACHUR4_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457203.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2380..>3527)
                     /locus_tag="ACHUR4_003840"
     CDS             complement(2380..>3527)
                     /locus_tag="ACHUR4_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1457204.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1072 a          776 c          622 g         1057 t
ORIGIN      
        1 atgctttttc gaaatttcag cggcctctat ttgagattta gcgatgccaa tctcgttaag
       61 attctctgaa agatataaat cacaggtgtc aatcttacct gatttaacca atggataatc
      121 cgttatactg taggtatctt taaaagccac ggcaaacatt tcaataataa caattactac
      181 cacaagcgaa atcaaaatgc gaaaaagagg aatactcctt ttgcgctcta ctttttcaac
      241 aacatcttct tgtaaatgag aatcatcata agattgttta cttattgtta tgttttcact
      301 gatgtaaaag cctactttgg gtatcgtaag aatcacattt tcttcaacac caatattttt
      361 aaaacttttg cgtaacaggg atatatattg gttcaatgtg ttgtttgaca gatcaaatga
      421 aaatctctcc aaaacgctat caatgattga gttcctgtca accgctccac cattactttc
      481 cagtaacact gccaaaatac accccgccgt agctggaaaa tttatctcat cctgggtatt
      541 tacattcgag agcgagcatt tgttcggatc aaaaataatc gcgtcattga taatgaaaaa
      601 acatctccgc tccatgcagg caacaacaca aaataagatt ttgtcatttt agcctattcc
      661 tggtggaact gacacaagaa actaaaatac aaatataacc ctgaaaaagt catataaaac
      721 ttatatccac agaataaaaa atcaaacatg tcagttttat caatataaag tatcgcaagg
      781 cgaaaagtga tgcaagaata ctcctaagta aatgaaagcg caccacatga aattatcaac
      841 acatgtaatt tcatcattag gtgctatcga aaataactca ggagctactt aagatgaata
      901 atgtcttact catgagaccc gatgactcag aatttatgat acctgatgac tggtcagggc
      961 taaacgcaaa aggcattaca tacgaacaag ataatagagt gacagagtct tttctgcctt
     1021 ttaaatcttt ttattgttca ccggtgtgca cgacagttga actcatctca taaaataatt
     1081 gttttgctat gggtatccgc taccttctgg aaaagaaaag agacttaagg ctaaatcact
     1141 ttaaagcctt ctctcataaa tgcgagacaa aacctgacgt gttaattatg accgggtgca
     1201 ccgtcgctga tacttatagt atattctcac gcattgttga agaaaatata atgaaaacga
     1261 tgacacctat cgtcatcatt actgggcatc actcagaaga tctttgttct ctggcatcga
     1321 aactgggagg agttaaaata atatcaccga cagaaaagat tgcaaaaata gaaaacgcta
     1381 tcaatgatgc ttttaacagc aagcctaaaa aaataagatt tttaacagat atactctctc
     1441 ctcaacaaat tgagaccatg aaaatggttg cgcagggtag atccgttaat gagatttcag
     1501 ccgcaatgaa tattagcatc aagaccgtgt atgcacataa agttcgtgct atcaataagc
     1561 tgggaattaa gaaaaaagtt tatgaagcgc ttttttataa agcgctaatt cagttcaact
     1621 gatgtaaaaa tcaccctcgc ttaataaata atatattcta tgtctttttt tataagttaa
     1681 aaaaggcggg atatcaaatc ccgccatgaa attaactaat catctgcatt attctgcgta
     1741 gatataagcc tgggtacgga tggtgtaaga gtctggctca acaacctgtt ctttcatatg
     1801 ggaaacgacg cgataggcac cacctgttcc agtatttttc agcatcatat aacgcttacc
     1861 gccaatctct tcggaggcag ctgaaggagg atcctgctca agcgttgtca gggaaacttt
     1921 cagtttatgg tccgggttat tgtcaccaga aattactgca tccgctgaag tactgctctc
     1981 cgcataaata tcatccggga aagttacagc atagtcgtgg ctctgcccat cagatgcgcc
     2041 aacaatacct gttgccaaag gcgtccatgc agttaacgta ccgctatata cccctttcac
     2101 tggttgaatg gtattattaa caacgatagg cttggtcaaa gtaagattag ctgacgcttc
     2161 agtaccctgg cttactaaag ttcccgcctg agaagcaaaa gctgtccccg ccagcatgat
     2221 tgctaacaat gttttcttca ttatattttc tccatgattt tcatttacac tgcaaaatgc
     2281 agtttctttc tttgcattta cgtgctatcg cgaaaatgct tccaccaaat aatggaaaca
     2341 tcttcacaaa aataaattat gagtttctta atttagaaac tataaacatg tcgccgctat
     2401 ctggtataac cctgatgcac ttttcttcgc aggaagttgg tattttgcga tacaggattg
     2461 atctttagaa taaccccatt tcacattaaa ctcgccttcc gcactcaagc cagaaacata
     2521 agcaatacca tcttcaccta caatgccggc catattttca gtgtctttgc tataactaat
     2581 cacagcgccg aaggggatgg gtttaccctc tttctgtttt aaggttacta atcctttatc
     2641 cccgctcttc actgccaatg tcgcaggcac aacggctcca gctgtcggta caaccaattg
     2701 actagtttgt ggtaagtcgt aattcttacc tatcgttttt tcatccagaa taacatcgtc
     2761 tcttcggtat ggctgcactg gcagtattgc atacccacga aaatcagtac tcagtcccgg
     2821 ccagccatta acgttaaccc ccgttgctcc gggtgcctct ataagggcta atgtcccatc
     2881 cacattttga ccaaatgtaa gtccatccct tgtaataagt atcccaccgc ttaaatttgc
     2941 acctagctgt ttcatgtttt cgctggagct gtaatggcta ctaaactgac catatgatcc
     3001 atttttgcca acttgcacaa tgttcatcgt gctcttcggt tgtgactgat gagcgttagt
     3061 tatatttgtc gctacatccc accatacacc accactattc tgacctgata atgaaacctg
     3121 atagttatta ccctgctctc ccgaacgact catctgtgaa gacgcataaa tttgattttc
     3181 gtcacccagc gataatggca ctgaaatacc cacgcttaat ttccgatcta ctttccattt
     3241 atgcgatgtc agcaagtcgt tttttgccca gttaatggag aatgttatgc ctctccatac
     3301 cgtactataa ctcaggctgg tagaccgcgt gtttcctgtg ccccgggaag aattatacct
     3361 cgtggtattg aagttcaaat tcccgatatt accaaggggc tgggataagg atatcgtaga
     3421 gccatcgtga ctattcttat ctctttttct gttccgttca tcctgtgcac ttactgcttc
     3481 agtcagtgtg gcataacctg atgcataacg gttcgtggtt atagaca
//
LOCUS       DAZPFP010000256         3499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPFP010000256 DAZPFP010000000
VERSION     DAZPFP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.261"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..313
                     /locus_tag="ACHUR4_003841"
     CDS             <1..313
                     /locus_tag="ACHUR4_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR1457205.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            470..1219
                     /locus_tag="ACHUR4_003842"
     CDS             470..1219
                     /locus_tag="ACHUR4_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1457206.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1241..1807
                     /gene="yieF"
                     /locus_tag="ACHUR4_003843"
                     /gene_synonym="chrR"
     CDS             1241..1807
                     /gene="yieF"
                     /locus_tag="ACHUR4_003843"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1457207.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1861..3198)
                     /gene="adeP"
                     /locus_tag="ACHUR4_003844"
     CDS             complement(1861..3198)
                     /gene="adeP"
                     /locus_tag="ACHUR4_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1457208.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3363..>3499
                     /locus_tag="ACHUR4_003845"
     CDS             3363..>3499
                     /locus_tag="ACHUR4_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1457209.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          866 g          764 t
ORIGIN      
        1 ggcactggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta tgagcctgcc
       61 ggaattcgag cagtcactgt ttatggcagc gcaacccgac aatctgctac tggcgaccgc
      121 gccgcgctac tgtcagtact acaatcaact ccatcaactg ccgttggttg ctcttcctct
      181 cccgtttgac gaaagccagc aaaaaaagct ggaagttcct tttaccctgc tgtggcataa
      241 acggaacagc cataatccga agatcgtctg gttacgggaa accattaaaa acctttacgc
      301 gtcgatggca taaccgaatc gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa
      361 gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt
      421 aaatgattac tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca
      481 ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg
      541 tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg
      601 tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg aaatcgtcac
      661 cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt ctatttccta
      721 tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga
      781 actacagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc acaccttttc
      841 cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct
      901 cattacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg acgatccgcg
      961 cgatctgcag ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga
     1021 agcgttatgc gacgcggagg acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga
     1081 aaagctcagt gtctgggagt tagatggcaa acacagctgg aaaagcctgc cggatattca
     1141 cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct ctaccgtgaa
     1201 gtctttttcg ccaaattgat agacaaccac aggagtcatt atgtctgaaa aattgcaggt
     1261 ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct
     1321 gccgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg ccgacattcc
     1381 cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg aagctctggc
     1441 ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata actactcggt
     1501 accgggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc aaccgctggc
     1561 cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg cgcgctgtca
     1621 gtatcacctg cgccagattc tggttttcct cgatgcaatg gtgatgaaca agccggaatt
     1681 tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga ttgatcaggg
     1741 tacgctggac cacctgaccg ggcaattgac cgcatttggt gagtttattc agcgagttaa
     1801 gatctaaata aaaaacccgc cagtaatcat gcatggcggg tttttaatgc gctatcgatt
     1861 ttaatgagcg tcgataaata caatcttcag gataaacagc agcgcaacga tgattacgca
     1921 cgggctaagg tcacgcagac gcccggtacc aatcttcatc acgcagtagg agataaagcc
     1981 cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc atcacggcgg taataaacgc
     2041 cggaacagat tcagtaagat cctgccagtt cacgcgagca agacttgagg tcatcaacac
     2101 gccaacgtag atcaacgcgc cagctgcagc gtagtctggc accattcccg ccagcggcga
     2161 cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac cggtacgacc
     2221 gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag taccgataaa
     2281 cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc gcgggaattt
     2341 ccccttctca tccgccagac ctgctttatc ggtcacgcca atcagcgtac cggaggagtc
     2401 aaacaagttg accaacatga aagagaaaat cacccctgcc agcccgaggt taaacgaccc
     2461 ggctaaatct acatgaccca caactgtcat tacgctcggc ggcgcagaaa cgatgccatt
     2521 gtagtgcaca tcacccagca tccagcccag cagcgtcgtc accacgatag aaaccagcac
     2581 cgctgcgtga atgttgcgcg aggccagaat agcaatgatg aaaaagccga ggatacccag
     2641 aagtacgctg tgagaagtca gattaccgat gctcaccagc gtttccgagt tagcgacaat
     2701 cacacctgcg tttttcagcc ccatcatgcc gataaacagg ccgataccgc tggtaatacc
     2761 cacacgcaga cttaccggaa tgttagcaat catccagtaa cgaacgcgga aaatggtcaa
     2821 aagaagcagt ccgacagcgc cccagaagat tgcgcccata ccgatctgcc acggcagccc
     2881 cattgcctgg acaaccacaa aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc
     2941 caccggaagg tttgcaaaca atcccatcat aatgctaccg aaagcagcaa tcagacaggt
     3001 tgttacgaag acggcactgg tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa
     3061 tacgatgtag accatcgtca ggaaggtggt gaacccggca atcacttcag ttcgtgccgt
     3121 agtaccatgt tcgcgcagtt taaaaacacg ttccagcatc ctctgaccag aagtctgggt
     3181 ggtatgttga tgactcatta tctatttccg aacaaggagg gaaaatccgt cgctatcgta
     3241 taccaaaatg cgacaatagg cgcgtttgtg agagactttt ttattggatt tacttatacg
     3301 gcaacgattg cgttgcgcaa atcggctcta cgaaaacgtt aaactgatta aaaaggaaag
     3361 gcatgtcccg gatagaagcg gtatttttcg actgcgacgg tacgctggtc gacagtgaag
     3421 tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg
     3481 aagaggtatt caaacgttt
//
LOCUS       DAZPFP010000257         3462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPFP010000257 DAZPFP010000000
VERSION     DAZPFP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.262"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHUR4_003846"
     CDS             <1..77
                     /locus_tag="ACHUR4_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1457210.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHUR4_003847"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHUR4_003847"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1457211.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHUR4_003848"
     CDS             1490..2614
                     /locus_tag="ACHUR4_003848"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1457212.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHUR4_003849"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHUR4_003849"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1457213.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          912 a          756 c          846 g          948 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tg
//
LOCUS       DAZPFP010000258         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPFP010000258 DAZPFP010000000
VERSION     DAZPFP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.263"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHUR4_003850"
     CDS             complement(226..429)
                     /locus_tag="ACHUR4_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457214.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHUR4_003851"
     CDS             complement(431..586)
                     /locus_tag="ACHUR4_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1457215.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHUR4_003852"
     CDS             complement(789..1196)
                     /locus_tag="ACHUR4_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457216.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHUR4_003853"
     CDS             1273..1500
                     /locus_tag="ACHUR4_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1457217.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHUR4_003854"
     CDS             1484..1906
                     /locus_tag="ACHUR4_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1457218.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHUR4_003855"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHUR4_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHUR4_003856"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHUR4_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZPFP010000259         3415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPFP010000259 DAZPFP010000000
VERSION     DAZPFP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.264"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUR4_003857"
     CDS             <1..239
                     /locus_tag="ACHUR4_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR1457219.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACHUR4_003858"
     CDS             249..887
                     /locus_tag="ACHUR4_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1457220.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACHUR4_003859"
     CDS             890..2053
                     /locus_tag="ACHUR4_003859"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1457221.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3415
                     /locus_tag="ACHUR4_003860"
     CDS             2137..>3415
                     /locus_tag="ACHUR4_003860"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1457222.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          804 c          987 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggtt
//
LOCUS       DAZPFP010000260         3404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPFP010000260 DAZPFP010000000
VERSION     DAZPFP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.265"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(80..715)
                     /gene="msrQ"
                     /locus_tag="ACHUR4_003861"
     CDS             complement(80..715)
                     /gene="msrQ"
                     /locus_tag="ACHUR4_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1457223.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(716..1720)
                     /gene="msrP"
                     /locus_tag="ACHUR4_003862"
                     /pseudo
     CDS             complement(716..1720)
                     /gene="msrP"
                     /locus_tag="ACHUR4_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            complement(1829..2242)
                     /gene="hiuH"
                     /locus_tag="ACHUR4_003863"
     CDS             complement(1829..2242)
                     /gene="hiuH"
                     /locus_tag="ACHUR4_003863"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1457224.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2375..3046
                     /gene="hprR"
                     /locus_tag="ACHUR4_003864"
     CDS             2375..3046
                     /gene="hprR"
                     /locus_tag="ACHUR4_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1457225.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3046..>3404
                     /locus_tag="ACHUR4_003865"
     CDS             3046..>3404
                     /locus_tag="ACHUR4_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HFR1457226.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSI"
BASE COUNT          917 a          755 c          831 g          901 t
ORIGIN      
        1 gatgcgatct gtgtgggccg gatagggcgc tcgcgccgca tccggccggg ataacgcgaa
       61 gtttgtcggc aatatgaagt tatagagata atttgttgtg ggcctgcttg cgtaaccggt
      121 tgaaaaggga aagcaacttc ttataccgta aggctaaaag cagtacagcc agcccggcgt
      181 agatgagtgg ctgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga
      241 tcgcgacaag atagacgaag ttatgcaact gttgccagtg tttgccgagt tttcgctgca
      301 tcgactgggt tgaagtgaac gctaaagcaa gcaaaataac ccagctgata attcctaacg
      361 ttaaataagg ccgggtaatt aactccttac ctaataacgc cagattattc acgcccaact
      421 ccagcaatgc gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca
      481 ggcggcgagt gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca
      541 aggccgcaag caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg
      601 gatcggcacc cagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc
      661 cggcaagatg caggcaaact ttcagccata tcacttgttt tgctgtcaga cgcatttaga
      721 aattctcccg caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa
      781 acagtagtgt tggctggcgc tgaacatcga gaatgccgcc tgaaccaata aatcgttcgg
      841 tagcctgtga ccagcgcggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt
      901 cgtcaggcgc tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga
      961 cgatcgattt aatcccttta aagccatatt tccacggcac aatcagtcgc accggcgcgc
     1021 cattttgcgg tggtaacgcc ttgccataaa cacctacggt catcagtgtg agcggatgca
     1081 ttgcttcgtc gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc
     1141 ggtcctgctg gccaggcatc tgttccggcg cataaatcgt ttcgaaagcg acatacttcg
     1201 cattgctggt gggttcggca agcgccagca atttgtgtag cggaaaacca atccacggca
     1261 ccaccatcga ccacgcttcc acgcagcgca tacgataaat acgctcttcc agcgggaagc
     1321 gacgggttaa atcatcatga tcgagggtca atggttttgc cacttcgccg ctgattttca
     1381 gtgtccatgg atcggttttc aggctaccag cattagccgc gggatcgtct ttatccagcc
     1441 cgaattcata gaagttgtta taaccggaga ctttatctgc tggcgtcagt ggcaggttat
     1501 tttgctaggc ggcaggcttg ctgaactcca gcggttttcc ggcgggggcg ggcggacgat
     1561 cgttcccttt aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaaatgcag
     1621 ctgcgctgat gcccagtgct tttaacacct gccgacgctt cataaagaat accgactcgg
     1681 ccgtaacatc tgattctttt aaaaattgct tctttttcat aagccatcac acttttttgt
     1741 cgttaaccaa caagagctgt ttactgttaa tctgtcaggg agtcacctcc ctaacagtag
     1801 cagagatcag cttttacata tttacgcatt aactgccacg ataggttgaa tacccatatt
     1861 ggctgagtaa caaaggcaca tgataatgct cgttcacttt attaatatga aattcaacgg
     1921 gaatctcagg gaagaaactt tcaagatttt gtttcttgaa atagtccccg gttttaaata
     1981 cgacacggta atcgccagta gtcgcagttt gctcgggcca cagtgcctta attcgtccat
     2041 ccttatctgt tttggcggta ttaagttgta accagccgtt atccgctttc ttttcaagag
     2101 tgactgtcac gtcggcagcg ggttttcctg tttgctggtt caaaatgtgc acgctaagaa
     2161 tgttttgttg tgcggcataa accaaagaag gtaatgaaaa ggctgccgtt gctaccgaga
     2221 gtactaaata acgctttaac atgtttatat ccttgtcatg tgaatgagtt ttgaggatag
     2281 cgaaaaaacg tccagggtaa ccttacagca gcattacaat tttgtaatga accaggttgt
     2341 ttctataaca tatgatttat ggcatattat tttcatgaag attctactta ttgaagataa
     2401 tcaaaggacc caggaatggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc
     2461 cgtttctgat ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat
     2521 tctggatatt atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacggc
     2581 aaagcaaacc cctgttattt gccttactgc aagggattct gtcgatgaca gagtcagagg
     2641 gttggacagt ggggcaaatg attatctggt aaaacctttt tcattttctg agttgctagc
     2701 aagggttcgg gcacaattaa ggcaacatca cgctctgaat tcaacattag aaatcagtgg
     2761 cttaaaaatg gactctgtca gtcaaagtgt cagcagggac aatatcagta ttacactgac
     2821 gcgcaaggag tttcagttac tttggctact ggcctccaga gctggcgaga ttatacccag
     2881 aacggttatt gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga
     2941 tgtcgccatt cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaatcgc
     3001 cacaatccgg gggatgggct attcattcgt agcggtaaaa aaataatgaa aagattatct
     3061 ataaccgtcc gtttaacctt gctttttata tttctgcagt ctgttgctgg cgctggaatt
     3121 gtatggactc tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc
     3181 attaaccgga cagcgcagat caagcagttg ttaattgatg gggtaaatcc agatacgtta
     3241 cctgtgtact ttaaccggat gatggatgtt agtcaggata ttttgatcat tcatagtgat
     3301 ggcatcaata aaattgttaa ccggacaaat gtcagtgatg acatgttaaa taacatacct
     3361 gctagtgaga caatcagcgc agcaggcatt tacagaagca ttat
//
LOCUS       DAZPFP010000261         3402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPFP010000261 DAZPFP010000000
VERSION     DAZPFP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.266"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            49..1200
                     /gene="yiaY"
                     /locus_tag="ACHUR4_003866"
     CDS             49..1200
                     /gene="yiaY"
                     /locus_tag="ACHUR4_003866"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR1457227.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1365..2903
                     /gene="aldB"
                     /locus_tag="ACHUR4_003867"
     CDS             1365..2903
                     /gene="aldB"
                     /locus_tag="ACHUR4_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR1457228.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3122..3265)
                     /locus_tag="ACHUR4_003868"
     CDS             complement(3122..3265)
                     /locus_tag="ACHUR4_003868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457229.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
BASE COUNT          847 a          829 c          909 g          817 t
ORIGIN      
        1 aatcctcagt aagctgccca ccctttttta cactttcagg agtgtgttat ggcatcttca
       61 actttcttta ttccttctgt gaatgtcatc ggcgctgatt cattgactga tgcaatgaat
      121 atgatggcag attatggatt tacccgtacc ttaattgtca ctgacaatat gttaacgaaa
      181 ttaggtatgg cgggtgatgt gcaaaaagca ctggaagaac gcaatatttt tagcgttatt
      241 tatgatggca cccaacctaa ccccaccacg gaaaacgtcg ccgcaggttt gaaattactt
      301 aaagaaaata attgcgatag cgtgatctcc ttaggcggtg gttctccaca cgactgcgca
      361 aaaggtattg cgctggtggc agccaatggc ggcgatattc gcgattacga aggcgttgac
      421 cgctctgcaa aaccgcagct gccgatgatc gccatcaata ccacggcggg tacggcctct
      481 gaaatgaccc gtttctgcat catcactgac gaagcgcgtc ataacaaaat ggcgattgtt
      541 gataagcacg tgactccgct gctttctgtc aatgactcct ctctgatgat tggtatgccg
      601 aagtcactga ccgccgcaac gggtatggat gccttaacgc acgctatcga agcgtatgtt
      661 tctattgccg ccacgccgat cactgacgct tgtgcactga aagccgtgac catgattgcc
      721 gaaaacctgc cgttagccgt tgaagatggc agtaatgcga aagcgcgtga agcaatggct
      781 tatgcccagt tccttgccgg tatggcgttc aataatgctt ctctgggtta tgttcatgcg
      841 atggcgcacc agctgggcgg tttctacaac ctgccacacg gtgtatgtaa cgccgttctg
      901 ctgccgcatg ttcaggtatt caacagcaaa gtcgccgctg cacgtctgcg tgactgtgcc
      961 gctgcaatgg gcgtgaacgt gacaggtaaa aacgacgcgg aaggtgctga agcctgcatt
     1021 aacgccatcc gtgaactggc gaagaaagtg gatatcccgg caggcctacg cgacctgaac
     1081 gtgaaagaag aagatttcgc ggttctggcg actaatgccc tgaaagatgc ctgtggcttt
     1141 actaacccga tccaggcaac tcacgaagaa attgtggtga tttatcgcgc cgcaatgtaa
     1201 tcatcattcc aacaacggct ggcaaattgt cagccgcttt ttcaactatc tctgtaatcc
     1261 ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc gactggcgaa gatttcgcca
     1321 gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc aataatcccc
     1381 cttcagcaca gattaagccc ggcgagtatg gtttccccat caagttaaaa gcccgctacg
     1441 acaactttat tggcggtgaa tgggtagccc ctgccgatgg tgagtattac cagaatctga
     1501 cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga gacatcgatc
     1561 tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg gtgcaggatc
     1621 gcgcggcgat tctgtttaag attgccgatc gaatggaaca aaacctcgag ctgttagcga
     1681 cagctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcg gatgtaccgc
     1741 tggcgattga ccatttccgc tatttcgcct cgtgtattcg ggcgcaggaa ggtgggatca
     1801 gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc gtggtggggc
     1861 agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct cccgcgctgg
     1921 cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct gtactgctgc
     1981 taatggaaat tgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc aatggcgcag
     2041 gtggggaaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg gcgtttaccg
     2101 gctcaacgga agtgggccaa caaattatgc aatacgcaac gcaaaacatt attccggtga
     2161 cgctggagtt gggcggtaag tcgccaaata tcttctttgc tgatgtgatg gatgaagaag
     2221 atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac cagggcgaag
     2281 tttgcacctg tccgagtcgt gccttagtgc aggagtcaat ctacgaacgc tttatggaac
     2341 gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc gctcgacagc gtgacgcaaa
     2401 tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt gatatcggta
     2461 aaaaagaggg cgctgacgtg ctcacaggcg ggcggcgcaa gctgctggaa ggtgaactga
     2521 aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg cgggtgttcc
     2581 aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg gaagaagcgc
     2641 tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc cgcaacggta
     2701 atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc aactgttatc
     2761 acgcttaccc ggcacatgct gcgtttggtg gctacaaaca atcaggtatc ggtcgcgaaa
     2821 cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg agctactcgg
     2881 ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt cattgcctga
     2941 tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg gataaggcgt
     3001 acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt taaacctacg
     3061 tgcgcaataa cccagactac gccaacagaa gtgcgtttct ggggcgtgtt ttgtcaggag
     3121 tttaatctcg cgtttcgatg gtgagcgggc gaaccaggcc gtaaatcacc attggtgatc
     3181 cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgctta atctgtactg
     3241 tggatgtttt actggagtta ttcattagtt tggccaatag ttccactatt tcaatatgat
     3301 aaacctcgtc ctgttgaaaa accagacagc acatttcaga aagtatataa aaaacaatat
     3361 gtcgttatat aaataaggaa attgcactgc atcatggcat tt
//
LOCUS       DAZPFP010000262         3373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPFP010000262 DAZPFP010000000
VERSION     DAZPFP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.267"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHUR4_003869"
     CDS             <1..1202
                     /locus_tag="ACHUR4_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1457230.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHUR4_003870"
     CDS             1216..2127
                     /locus_tag="ACHUR4_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR1457231.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHUR4_003871"
     CDS             2127..>3373
                     /locus_tag="ACHUR4_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR1457232.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZPFP010000263         3370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPFP010000263 DAZPFP010000000
VERSION     DAZPFP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.268"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHUR4_003872"
     CDS             108..2105
                     /locus_tag="ACHUR4_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR1457233.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..>3370
                     /locus_tag="ACHUR4_003873"
     CDS             2168..>3370
                     /locus_tag="ACHUR4_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1457234.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHI"
BASE COUNT          790 a          780 c          856 g          944 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata
//
LOCUS       DAZPFP010000264         3347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPFP010000264 DAZPFP010000000
VERSION     DAZPFP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.269"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            18..896
                     /locus_tag="ACHUR4_003874"
     CDS             18..896
                     /locus_tag="ACHUR4_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1457235.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            893..2284
                     /locus_tag="ACHUR4_003875"
     CDS             893..2284
                     /locus_tag="ACHUR4_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1457236.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2281..2538
                     /locus_tag="ACHUR4_003876"
     CDS             2281..2538
                     /locus_tag="ACHUR4_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457237.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2633..2833
                     /locus_tag="ACHUR4_003877"
     CDS             2633..2833
                     /locus_tag="ACHUR4_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR1457238.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2826..3206
                     /locus_tag="ACHUR4_003878"
     CDS             2826..3206
                     /locus_tag="ACHUR4_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1457239.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          725 c          969 g          768 t
ORIGIN      
        1 attctgaggt gacagcgatg atgacgttta acctgcgtga acaacaaaaa agactacagg
       61 cgcgaatgga tgagttacgg gcagagattg catttgctca gaagggcgaa aagccatggc
      121 cttatcgttc ctgcctgatg cgtgaaggtc gcggatattg cgaaaaacat ggcgaatatc
      181 acacgcatat actggtgtgg agcgatcgta atggcgagga cagagaagaa atttcatgct
      241 gccctgactg cttaatcgct gaggccaacg atttgaccat ggagctgtcg tccatcaagg
      301 cggaagagct gactgataac gccggaattg ccctgcgttt tcgggactgc gagtttgata
      361 attatctgga ggttaatcct ggcgcagcca gaaatcttgc ggcctgtcgc cgctatgcgg
      421 agaactggcc agatatgctg gagaacggta ccagtcttgt tatgaccggc agttgtggta
      481 ccggaaaaaa tcatctggct gtggctatgg caaaacacat catccgtaac tatctggcca
      541 gtgtggagat caccgacgtg atgcgcctta cccgtgctgt gaaaaattgc tggcggaatg
      601 acagcgaaaa aacagcggat gaagttattg agcgttatgc gtcaatggat ttgctgatca
      661 tcgacgaagt tggcgttcag tttggcagcg cggctgaaat ggctattttg caggaaatta
      721 tcaacgccag gtacgaaagt atcttgccca ccattctgat cagtaacctc tcaccggaag
      781 agttgtgggc gttcatcagt ccccgaattg ccgacaggat cacagacggg ggacgcaact
      841 ggttgtcgtt taactggccc agttaccgtt ctcgtatcag aggtgtggct gcatgacaac
      901 tccagtatgg cgtaacgatg accttgaagg cgctgtcatc ggcgcgttct ttctgcgcgg
      961 agcagatcct gaagtgatgg atattctggc cacactgcca gcggacgttt tttctgtacg
     1021 agcgtatcag gatatctata caggcatctg cagacaggcc cgtgtttcag gagtgattga
     1081 ccccgtgctg ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg
     1141 taaaacctgg gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc
     1201 actgcgcgat gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac
     1261 ctgtgaagca gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac
     1321 aggaaagggc gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg
     1381 tgttgaatgc cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga
     1441 actggacgca aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc
     1501 ttcgatgggg aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca
     1561 tggtgtgctt ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc
     1621 tgcggctgga gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga
     1681 ctggacgcgt ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga
     1741 ccaggcgaac ctggccattg atgagatatg cgcaacaacg aaacaccacc tgattaaata
     1801 tccggaaacg gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg
     1861 gcgtcatgac cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg
     1921 tggttttccg ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa
     1981 acgtcccatg aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat
     2041 tctgatgctt tacagggatg aagtgtacaa cccggatacg caggccaggg gcatcgcaga
     2101 aatcaatatc acgaaacaac gtaacggttc tctgggaacg atttaccggc gtttttataa
     2161 cggacatttt ctgcctgtgg accaggaaag tgcacgggtg ctttccactc ccatgaagcc
     2221 cggcaatccg cgcagataca gtaataaacg aactgacagc agtaagatgg agcgtttctt
     2281 ttgaacaacc agacaatgac ttttacccct gaacaattgc gtaaacacgc gcaggaaatg
     2341 ctgcgtcatg ctgaacaact cgaaaaaacg ggcataacaa aagatgctat ccgtaaagat
     2401 atggtacccg cgcttcggga actgatgcag gcgaagcatc gcgcacagaa agcagtagat
     2461 gagctggtgg actgtgtggc agagctggaa accagagtcg gaaagtttga aaaactggtg
     2521 caggaggcgc tgcgctgatg cgccatgagt ttgttttgcc ctggcttgtg cgcgatcatt
     2581 atgtccctgg tggaaagccc gggataaaaa tcaccgaacc ggagagtatt tgatgagcgt
     2641 caaaattcaa acgataccgg aactgcttat ccagacacga ggtaatatga ccgaagtttc
     2701 acgaatgctg aactgtaatc gcgccacggt aagaaaatat gctgaagata aagagggcaa
     2761 agggcacgcc attgttgatg gtgttctgat tgttcatcgt ggatgggaca ggggtaaaga
     2821 cagtgatgcg tgatattcag caggttcttg agcgctgggg cgcgtgggtg gctaataatc
     2881 atgaggatgt gacctggtcg tccattgctg ccggttttaa gggattaatc ccttcaaaag
     2941 taaaatcccg cccgcaatgc tgtgacgatg acgcgatggt tatatgtggg tgtatggccc
     3001 gccttaaccg gaacaacaac gatctacatg acttgctggt tgactactac gtgtttggga
     3061 tgacattcat gacgctggcg cggaagcatg gatgctcaga tggttatata ggaaagaagc
     3121 tacaaaaagc cgagggggtg gtggaaggaa tgttgatgat gttgggtgtc cggttagaaa
     3181 tggacaaata tattgaacat ccctgatggt aaggggcgac tttctagaaa atattttacg
     3241 tacgtaaaaa acgggatatt ctgttaagag tggttgcttc gccacacagc ttaaacccgc
     3301 ctccgggcgg gttttttatg cctgaaaaac ggtacaggac gttaaac
//
LOCUS       DAZPFP010000265         3326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPFP010000265 DAZPFP010000000
VERSION     DAZPFP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.270"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..209
                     /locus_tag="ACHUR4_003879"
     CDS             <1..209
                     /locus_tag="ACHUR4_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457240.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            270..458
                     /locus_tag="ACHUR4_003880"
     CDS             270..458
                     /locus_tag="ACHUR4_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457241.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            455..1282
                     /locus_tag="ACHUR4_003881"
     CDS             455..1282
                     /locus_tag="ACHUR4_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1457242.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1326..2075
                     /locus_tag="ACHUR4_003882"
     CDS             1326..2075
                     /locus_tag="ACHUR4_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1457243.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2072..2641
                     /locus_tag="ACHUR4_003883"
     CDS             2072..2641
                     /locus_tag="ACHUR4_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457244.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2638..2760
                     /locus_tag="ACHUR4_003884"
     CDS             2638..2760
                     /locus_tag="ACHUR4_003884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457245.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2765..3007
                     /locus_tag="ACHUR4_003885"
     CDS             2765..3007
                     /locus_tag="ACHUR4_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1457246.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3011..3157
                     /locus_tag="ACHUR4_003886"
     CDS             3011..3157
                     /locus_tag="ACHUR4_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457247.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3166..>3326
                     /gene="xisR"
                     /locus_tag="ACHUR4_003887"
     CDS             3166..>3326
                     /gene="xisR"
                     /locus_tag="ACHUR4_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1457248.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          929 a          797 c          854 g          746 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca
      421 tttataaggg tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc
      481 ccttgttctg ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg
      541 catcaaggta aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg
      601 ctactggacg ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac
      661 ctacgatgag ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg
      721 cgtggcctac aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg
      781 cgtggacgac attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact
      841 ggccagcaaa atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc
      901 agctcttaaa gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc
      961 atatgtgggt aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac
     1021 ggcgaaccag aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa
     1081 catgttgcgc gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga
     1141 agccgatgct attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct
     1201 ggaagccatc aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac
     1261 accatttatt caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga
     1321 taaaaatgaa tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc
     1381 agcgtgttgt gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc
     1441 gtgcagcatt caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag
     1501 gtgcggacgt aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg
     1561 ggattgtact ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg
     1621 cctggcaggt tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg
     1681 aaatcgagat gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc
     1741 atgtggaaga acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata
     1801 tgcgcgccgg atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca
     1861 agtgtcggaa tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc
     1921 cggacggtct gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc
     1981 accaaatgat gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc
     2041 ttttccaggt gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc
     2101 cgctctggtg tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac
     2161 gccgaaacat attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg
     2221 gacgaagaaa aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac
     2281 agagctgatt tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact
     2341 gtccattacc ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc
     2401 gaaaaagttt gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga
     2461 gaagatgttt ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg
     2521 gcaagcggtc agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt
     2581 tgttgtgatg ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg
     2641 aaaatcgaat accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta
     2701 actctgggga tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa
     2761 ccacatgttc gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt
     2821 agaaattact cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg
     2881 gcgagtacgc atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca
     2941 caagattctg gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag
     3001 gaagtaaaaa atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta
     3061 cgacctgacg cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga
     3121 ggtctggtgc cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt
     3181 caactggctc ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa
     3241 ccaggcacaa ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac
     3301 gtatcaccag acggtaatcc aaagcc
//
LOCUS       DAZPFP010000266         3324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPFP010000266 DAZPFP010000000
VERSION     DAZPFP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.271"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHUR4_003888"
     CDS             complement(<1..171)
                     /locus_tag="ACHUR4_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR1457249.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUR4_003889"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUR4_003889"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1457250.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUR4_003890"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUR4_003890"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1457251.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUR4_003891"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUR4_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR1457252.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHUR4_003892"
     CDS             complement(2840..3175)
                     /locus_tag="ACHUR4_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1457253.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          700 a          853 c          936 g          835 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttcatttga aaggaaacgc aaaa
//
LOCUS       DAZPFP010000267         3311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPFP010000267 DAZPFP010000000
VERSION     DAZPFP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.272"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..121
                     /locus_tag="ACHUR4_003893"
                     /pseudo
     CDS             1..121
                     /locus_tag="ACHUR4_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(151..3063)
                     /locus_tag="ACHUR4_003894"
     CDS             complement(151..3063)
                     /locus_tag="ACHUR4_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR1457254.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3120..>3311)
                     /locus_tag="ACHUR4_003895"
     CDS             complement(3120..>3311)
                     /locus_tag="ACHUR4_003895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457255.1"
                     /translation="MFTSEQHRYSNEKKEFQRRVYLRGDAFINLYTNFYSCIMTGLQL
                     LRHFQRIALPAYHPTFFIP"
BASE COUNT          832 a          884 c          800 g          795 t
ORIGIN      
        1 cccggtcagt taccgcgcac atggcctgcc cgtgtcagaa aatctgctgg agcaggattt
       61 ttacgccagt ggtccgaacc agaagtgggc gggagacatc acgtacttac gtacagatga
      121 agttcggtta catcctgtca gcacggaacc tcacgctttt tgagcggcac gacggatccc
      181 ccccacccag tatccgacag acttttcact gcgttcgtaa aaacccttct tccctcccag
      241 catatccact aattccggtg acatatcctg cagataagtc tgcaaatcat gccccagatc
      301 ttccggtgac tgcaactcat aaaccgcgcg ccacacatca cccttcctta ttttaagccc
      361 cgggttaaga atatactgtc cgtggttcag cctcagaaaa agcttttttc cataaggatt
      421 tttgctgtta acttcatgtt gagagagctg gctgctgata taactccttc gtttgcggcg
      481 ttccggcagc aacgcttcag gaagatgaac cacacactct gccagtcgtg ccgcactgaa
      541 ggcttccgaa aaaaacagag cacttcccag atgcgtgtgg aacagcgcct gcatcaggat
      601 caccagaaac attgccatct gccggttgtc cagtttgatg aggcgtcctt ccacctgcag
      661 tgacagactt tcaggtttca gcagggtata caactgcgca cttttctgtt gcgcataacg
      721 gggcgtactg accgccgctg acagaacttg atgataagcc gccagcccgt cattaccggt
      781 cagcaaggga tctgcaccac gctccagcag taactgaacc agtgcaatat tacctgcccg
      841 ggctgctgac atcagcggag taagattaaa cggcgaacgg tgatcaacac cataacgttc
      901 aatatcccgc aacacggcaa ccgggttatt cattgagtat ggccggaaat acttcagctc
      961 cagtaactgc agagtcttcg ttctgggctg ccttgtgggc ttaaaatctt cccggtacag
     1021 tgcattatac agcgggaaca gggaataaat cagactgtac tccagcgcct gcagttgtat
     1081 ggttttatcg gctgtacctg cgaggatatg ctgtttccac tgtcgtgcat cctctgcggt
     1141 gataatctgc catggcatct cagatgtctg caggatccgg ctacggatgg tgtccgcctg
     1201 ctcctgcttc ccctgttttt caagcagatg tgcggttttc tgccactcat cacggctgga
     1261 aatctcttcc tgcagattca gaggctcttc ctgatgcgtg agcgcaagca gactccacag
     1321 aggatgctcc acctgctgtt caacaagata cacattgtgt ttagcacggg tgagcgcaac
     1381 atacagcgcg ttggtaaaaa atttatagat ttccgctgaa cgatcctgcc tgtcccgcgg
     1441 acgggaataa cgggcctcgc cctcaagatc agccggtgta agaccctcgc aaatatcatc
     1501 aaatgcctgc cgggcagcgc ttactatatt gtaaaggatc accgtttcgt attccagccc
     1561 cttaacctcc tgaaccgaaa agaccagcgg cgtactgaac cagcatcgcg cctgtgcttt
     1621 ctgttccgga tgcatcacaa tgaccgctac acgattcgaa agtgaggttt tggcattcag
     1681 ttcctgcttt gtctcttccc tatcgtccag caaccggatg gtcccttctg cctgaccgca
     1741 gcttctgaca aaatggtgac tttcccgatc aatagcacta aagcggacct gtttgagtct
     1801 cagcaaccgg ttagccagtg cagtgacgtg gtgcccgtta cggtaattcg cctgcaggat
     1861 attaacggtt gtgtcgtttc cctgttgctg ccggaaaaag agacttttga gcgaactcca
     1921 tgagaaaaag ctcggatgaa ctatctgatt ggcatcacca cagagtaaaa actgaccggg
     1981 atgacgtagc gttttcagta ccagttgcag ttgaacaggc gtcatatcct gaacttcatc
     2041 aacaaaaatg acatcataac ggggagttgc caacgaaaga tactgatgac tgagcagatt
     2101 actgtcatac tgatgcgact gtttcagcca tgcgatataa cgttcaaaca ggacatagac
     2161 cgtcgggcgg tcctccatgc catagagcga ctgccgtatc cccagtgaca gatacgcctc
     2221 ccgggacagc ggcccattcc cggaggcaac cgcaccgatc acgcccctga actcctcata
     2281 cagggtatgg gcagccccca gtgcggcgat agccctgttg cggggcagcc agtcgctgaa
     2341 cgcactgaaa gtcacctccc tgccttccgg aatgcgcaga gtttccagaa attccgtcag
     2401 cgaaagaaag tcaatattct gtacctcact cccttcccct gaagcgtcat agagagtacg
     2461 cgctttttcc accagaaatg aggaaaggct gagatacagt atatcaccag cagcctgctt
     2521 cattttttcc agtaccagcg ccgtcttacc acttcccgcc gagccaacaa tcaccagcgg
     2581 tagcgggtgt tcgtacagtg cctgctgatc atcatcaaag gacagcattt tatccaggcg
     2641 gtggaaacgc ccatgagacg gattgatata cgtcagcgct tcagtcgcga tatcaacagg
     2701 atccggcact ggttgttgtt gtaagcgccc ttcatcaatc accacgtttc tgcgaagaaa
     2761 acgtgaagtg ttataggcat gattgcgaat atattccaga acaaggcaga tagtttcacc
     2821 gtggtagcga tacagcgaaa acagtaatcg gtcccggata ctgagccggg cgcggtaaag
     2881 attggtgtca attttacgaa catcagcctg aacaaagtta tcggtggcaa tagcctgtgc
     2941 aaatttttta taacccggga tcgtgtcagg attcagagta tgataaagac agattttcat
     3001 ggctgccggt ttccgtcgtg caaaaaacat gctgatttta cgaaggaatg ccttactgaa
     3061 caaccttgcc cggaataccc cccgaaatca gatggaacaa tcagaaaaat gctctgcagt
     3121 tatggaataa agaaggtggg gtggtacgcc gggagggcga tccgctggaa atggcgcagt
     3181 agctgcaggc ctgtcatgat gcaggaatag aagttcgtgt acaggttaat gaaagcgtca
     3241 ccccgtaaat aaactctgcg ctgaaattcc tttttttcat tggagtacct gtgttgttct
     3301 gaggtgaaca t
//
LOCUS       DAZPFP010000268         3286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPFP010000268 DAZPFP010000000
VERSION     DAZPFP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.273"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(3..299)
                     /locus_tag="ACHUR4_003896"
     CDS             complement(3..299)
                     /locus_tag="ACHUR4_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1457256.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            514..1242
                     /gene="tonB"
                     /locus_tag="ACHUR4_003897"
     CDS             514..1242
                     /gene="tonB"
                     /locus_tag="ACHUR4_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1457257.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1282..1680)
                     /gene="yciA"
                     /locus_tag="ACHUR4_003898"
     CDS             complement(1282..1680)
                     /gene="yciA"
                     /locus_tag="ACHUR4_003898"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1457258.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1785..2324)
                     /locus_tag="ACHUR4_003899"
     CDS             complement(1785..2324)
                     /locus_tag="ACHUR4_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1457259.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2354..3097)
                     /locus_tag="ACHUR4_003900"
     CDS             complement(2354..3097)
                     /locus_tag="ACHUR4_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1457260.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3125..3214)
                     /locus_tag="ACHUR4_003901"
                     /pseudo
     CDS             complement(3125..3214)
                     /locus_tag="ACHUR4_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          938 a          815 c          790 g          743 t
ORIGIN      
        1 actcagaaca ctttcttaaa tggtttcact gaaacgtgtt catagactcc tgccgctacg
       61 tacgggtcag catcggccca ggcctgagct gcttccagcg attcaaattc agcaataacg
      121 gttgagccag taaatcccgc agcccctgga tcgttactgt ctaccgctgg cattggacca
      181 gctgtcaaca aacgaccttc atcatgcagt aactgtaaac gtgctaaatg tgccggacga
      241 acggaaaggc gtttttcgag gctatcagct ttgtcttgag cgtaaataac atacaacacg
      301 ggcacaactc cttgttcggg aaagttgtaa agtacgttat ttgaaagggc gaagatctgc
      361 aacggaaaga tgatgtcttt gttaaggtca tgcataaagt aagggtaatt acgccaaaaa
      421 tgacattttc actgatcctg atcgtcttgc cttattgaat atgattgcta tttgcattta
      481 aaatcgaggc ctagtttttc tactgaaatg attatgactt caataaccct tgatttacct
      541 cgccgcttcc cctggccgac gttactttcg gtctgcattc atggtgctgt tgtggcgggg
      601 ctgctctata cctcggtaca tcaggttatt gaactacctg cgcctgcgca accaatttct
      661 gtcacgatgg ttgcgcctgc tgatctcgaa ccgccacaag ccgttcagcc gccaccggag
      721 ccggtggtag agccagaacc ggaacctgag ccgatccccg aaccgccaaa agaagcaccg
      781 gtggtcattg aaaagccgaa gccgaaacct aagccaaaac cgaagccggt gaaaaaggta
      841 caggagcagc caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa
      901 aatacggcac ccgcgcgccc cacatcaagt acggcaacgg ctgcaaccag caagccggtt
      961 accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta tccggcacga
     1021 gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgacgtcac gccggatggt
     1081 cgcgtggata acgtacaaat cctctcagcc aagcctgcga acatgtttga gcgtgaggtg
     1141 aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg gattgtggtg
     1201 aatatcctgt ttaaaattaa cggcaccacc gaaattcagt aagcagaaag tcaaaagcct
     1261 ccgaccggag gcttttgact attactcaac aggtaaggcg cgaggttttc cttcaggatc
     1321 aaccgctaca tacttaaata atgcttctgt cgctttatag cgttgcccga ttggttcaga
     1381 cgctactttt ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc ctttctggac
     1441 acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc
     1501 ttcaacccgc acagtcacta cgcgaccgtg ggcaatttct tttgccagaa tagcgccgcc
     1561 aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat tggtatcggc
     1621 gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg ttgtagacat
     1681 ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt tcctgaggag
     1741 aacagaggag atacagcgcc agccccgaag gactggcagt ctggttagga tttatcttcc
     1801 tgcggcatgt ggcggtagat atagataccg cttaacaatg tgaagattaa ggtaagggcg
     1861 gtcaggccaa agactttaaa gttgacccaa atattttgcg gcagccagaa tgcgatgtag
     1921 atgtttgcca gaccgcaaag gataaagaaa acagcccagg ccagatttag cttcgaccat
     1981 accggttgcg gcagcgtgag ttctttactc agcatccgct gaattagcgg ctttttcatc
     2041 acccattggc tgactaacag ggctcccgca aacagagcat aaatgactgt aaccttccat
     2101 ttaataaact catcattgtg gaagaacagc gtcaagccac cgaagacgac caccagaaca
     2161 aaagtgatca gggccatctt ctcaacctta cgaaagcgaa cccagctata gataagcaca
     2221 atcgccgtgg cgacgatcag cgctgcagta gccgcataga tgtcataaat cttgtaaaac
     2281 gcgaaaaaga caaccagcgg taaaaaatca agaaactgct tcattttacg attccgtaat
     2341 caaatgctta aggttattgg cgaatcaaca tatacaggcg gaacagatag atgagcaata
     2401 tggcagaaat caggttgctc aaggtgttcg ccagtacggc accaatttcc ggggttaatg
     2461 cggcaaaaga agaggcaaaa agcagcaaca gtgtttttgc cagcaaccag ctcagtactg
     2521 cgggtgccac cagacgcata ttcgcccaag tcagccgcat actgctacgc atcgaggcaa
     2581 aaatgcccat tttgtcctgt accagcatca ccggtgccag ggccagtaaa atggccatga
     2641 taattcccgg aacgaccacc agcatgatgc caatctgtac taaaagggta gtcagaaaaa
     2701 tcagaataaa taacttgggc aatatcggcg cactggcacc aatagctcgc agcgcactga
     2761 ctctctgacc cgcagacacc agctggataa ttaatattac gcctccggcg agaatggcgt
     2821 taccgattaa ttctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt
     2881 ccggtgacat attctgaacc aggtcgaaca acccactact gccgctaacg ggcacgccgt
     2941 cattgagctg cgcaagctgt gcatcactgg gtgagaaaac atgccctaac accactgtga
     3001 taaacgcaca tagcaacgat accaacagaa tggtcataaa ttgattacgg aagaaatttc
     3061 cggtgtcacg gtatacggac tgcgccgtga tagacatgca ctctccttga gttttatgca
     3121 ggtgttaatt agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg
     3181 tatggaaaag catatctttg taaagcggag gtaattgctg gccttcaggc gtgggcaaag
     3241 cgcggcaagt ggcgatcgag ttaatatttg cgtagagaaa atattt
//
LOCUS       DAZPFP010000269         3266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPFP010000269 DAZPFP010000000
VERSION     DAZPFP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.274"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            15..1055
                     /gene="gatD"
                     /locus_tag="ACHUR4_003902"
     CDS             15..1055
                     /gene="gatD"
                     /locus_tag="ACHUR4_003902"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1457261.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1161..1934
                     /locus_tag="ACHUR4_003903"
     CDS             1161..1934
                     /locus_tag="ACHUR4_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1457262.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHUR4_003904"
     CDS             complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHUR4_003904"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1457263.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          873 a          724 c          812 g          857 t
ORIGIN      
        1 aatcaggagt ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa
       61 aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg
      121 gcttatgtgg ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa
      181 cgttaggcca tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac
      241 accctggcga tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt
      301 tgaaagggtt ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg
      361 gatttgctga atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc
      421 ctattgagga tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag
      481 cgcaaggttg tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg
      541 ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag
      601 aaaaactggc actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga
      661 gcgcgccgca aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga
      721 cggctggcgt accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac
      781 tggcgctggt gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa
      841 tattgcgtaa agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg
      901 ggcaggagtg ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat
      961 taatcgctca ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta
     1021 atgccatgca gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc
     1081 ccggtattgt gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa
     1141 ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag
     1201 tgaatgaaca gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag
     1261 cgacaatccg tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg
     1321 gcggtgcggc gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta
     1381 aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa
     1441 tgatccatga agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg
     1501 aaggattaat gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg
     1561 ccctttccga aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa
     1621 cgcgctcgat gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa
     1681 tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt
     1741 attcgattag cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat
     1801 cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg
     1861 ttaccgatga tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat
     1921 taattacggt gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt
     1981 acggattctc cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt
     2041 ggtaatcgac aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac
     2101 ggttcgccat caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa
     2161 gcgccttcga taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga
     2221 agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgagttc
     2281 gggcacaatt gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg
     2341 tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc
     2401 atgcgcatta agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc
     2461 ggcgtttctg tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt
     2521 tgtttgttga cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc
     2581 gccttatcca gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat
     2641 ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact
     2701 tcattaatgg tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc
     2761 tcctctacaa atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt
     2821 ccttcctcac gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttacca
     2881 ttaagaatca gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt
     2941 ctgtagatag tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg
     3001 ataaaaagtc agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg
     3061 acagcgaata ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc
     3121 aactaaacag tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg
     3181 gcctcaatgt ccgtaattcc tacttatgta ggaaatgttg tacagaacat ttattataat
     3241 cctattcaat tataataatc atgcca
//
LOCUS       DAZPFP010000270         3253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPFP010000270 DAZPFP010000000
VERSION     DAZPFP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.275"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..576
                     /gene="yibB"
                     /locus_tag="ACHUR4_003905"
     CDS             <1..576
                     /gene="yibB"
                     /locus_tag="ACHUR4_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR1457264.1"
                     /translation="DESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVK
                     TPLVAWIDFGYCRKPNVTRGLKIWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNH
                     VYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYL
                     GRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            851..2047
                     /gene="kbl"
                     /locus_tag="ACHUR4_003906"
     CDS             851..2047
                     /gene="kbl"
                     /locus_tag="ACHUR4_003906"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1457265.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2057..3082
                     /gene="tdh"
                     /locus_tag="ACHUR4_003907"
     CDS             2057..3082
                     /gene="tdh"
                     /locus_tag="ACHUR4_003907"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1457266.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          797 a          717 c          859 g          880 t
ORIGIN      
        1 gatgagtcat ttacaaatag acttgagcct cgacaattaa aaaacccaga gtactggtca
       61 ccagagtatg ttttggtatg taaccttaag gcctattttg taaataaagc tatcaacatg
      121 ggtttagtga aaacgcctct tgttgcgtgg atagattttg gctattgccg taagccaaat
      181 gtaactcgag gattgaaaat ttgggatttc ccatttgatg aaaataagat gcatcttttc
      241 actataaaaa aagggcttac tgttacatct caacaacagg cttttgattt catgattggc
      301 aatcatgtat atattattgg gggcgcaatt gttggttcac agcataaatg gaaagaattc
      361 tataaattag ttctggaaag tcaaaaaata acactaaaca ataacattgt ggacgatgac
      421 cagggcattt ttgtcatgtg ctattacaaa cgttccgatc tcttcaacct aaactatctg
      481 gggagaggaa aatggttcga tttgtttcgc tgcttcagga gtaacacttt aggggcaaaa
      541 atgcaggcgc tgagaatttt cctttcaaga aaataggttt ccctccatta gtgggtagcg
      601 ttttgatgcc aactgagata ggaaaaatga tgctttggca tcattttttc tttttatatc
      661 aatgcagtaa aatcactcat cattatgctc agccaaaact gtacagtttt tctactatct
      721 gtaggtgttg agagacatca agatacacgt tatttatcct gaattttgca gaagtgttaa
      781 cgcatcatct cgtcgcgacc tataagtttg ggtaatatgt gctggaattt gccctgtctg
      841 gagaatcgca atgcgtggag aattttatca gcagttaacc aacgatctgg aaaccgcacg
      901 ggcggaaggg ttgtttaaag aagagcgcat tattacgtct gcgcagcaag cagatatcac
      961 cgtggctgat ggaagccacg tcattaactt ttgtgccaac aactatctcg ggctggcgaa
     1021 tcatccggat ctgattgcgg cagcaaaggc gggaatggat tctcacggtt tcggcatggc
     1081 ttcggtgcgt tttatttgcg gcactcagga cagccataaa gagcttgaac aaaaactggc
     1141 ggcattcctg gggatggaag atgcgattct ctactcttcc tgctttgatg ctaacggtgg
     1201 cctgtttgaa acgctgctgg gcgcggaaga cgccattatc tccgacgcac tgaaccacgc
     1261 gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc tatcgctatg ccaacaacga
     1321 tatgcaggag cttgaagctc gtctgaaaga agcgcgtgaa gccggtgcgc gtcatgtgct
     1381 gattgccacc gatggtgtgt tctcgatgga cggcgtgatt gccaatctga agggcgtttg
     1441 cgatctggca gataaatacg atgccctggt gatggtagac gactcccacg cggtcggttt
     1501 tgtcggtgaa aatggtcgtg gttcccatga atactgcgat gtgatggggc gtgtcgacat
     1561 catcaccggc acgctcggta aagcgctggg cggggcttct ggtggttata ccgcggcgcg
     1621 caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg tacctgttct ccaactcgct
     1681 ggcaccagca attgtcgccg cttccatcaa agtgctggag atggttgaag cgggtagcga
     1741 gttgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt gagcaaatgt cggcggcggg
     1801 ctttaccctg gcgggagccg atcacgccat tattccggtc atgcttggtg atgcggtagt
     1861 ggcgcagaaa tttgcccgag agctgcaaaa agagggcatt tacgtcaccg gtttcttcta
     1921 tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag atgtctgcgg cgcatacccc
     1981 tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt ggtaaacaac tgggcgttat
     2041 cgcctgagga tgtgagatga aagcgttatc caaactgaaa gcggaagagg gcatctggat
     2101 gaccgacgtc cctgtaccgg aactcgggca taacgatctg ctgattaaaa tccgtaaaac
     2161 agccatctgc gggactgacg ttcacatcta taactgggat gagtggtcgc aaaaaaccat
     2221 cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa gtggtaggta ttggtcagga
     2281 agtgaaaggc ttcaaaatcg gcgatcgcgt ttctggcgaa ggccatatca cctgcggtca
     2341 ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc aacaccattg gtgtcggtgt
     2401 taaccgtccg ggctgcttcg ccgaatatct ggtgattccg gcgttcaacg ctttcaaaat
     2461 ccccgacaat atctccgacg acctggcttc catttttgat cccttcggta acgccgtgca
     2521 taccgcgctg tcgttcgatc tggtgggcga agatgtgctg gtttctggtg caggtccgat
     2581 aggtattatg gctgcggcgg tggcgaaaca cgttggtgca cgcaatgtgg tgatcaccga
     2641 tgttaacgaa taccgccttg agctggcgcg caaaatgggt atcacccgtg cggttaacgt
     2701 cgcgaaagaa aaccttaatg atgtgatggc tgaactgggc atgaccgaag gctttgatgt
     2761 cggtctggaa atgtccggcg cgccgccagc gtttcgtacc atgctcgata ccatgaacca
     2821 cggcggtcgt attgcgatgc tgggtattcc accgtctgat atgtctatcg actggaccaa
     2881 agtgatcttt aaaggcttgt tcattaaagg catttacggt cgtgagatgt ttgaaacctg
     2941 gtacaagatg gcggcgctga ttcagtctgg cctcgatctc tcgccgatca ttacccatcg
     3001 tttctctatc gatgatttcc agaagggctt tgacgctatg cgttcgggcc agtccgggaa
     3061 agttattctg agctgggatt aacacgaaca agggctggta ttccagccct tttatctgag
     3121 gataatctgt taattatgta aaatcctgtc agtgtaataa agagttcgta attgcgctga
     3181 tcttttatat agctgttctc attatctctc taccctgaag tgactctctc acctgtaaaa
     3241 ataatatctc aca
//
LOCUS       DAZPFP010000271         3231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPFP010000271 DAZPFP010000000
VERSION     DAZPFP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.276"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            31..366
                     /locus_tag="ACHUR4_003908"
     CDS             31..366
                     /locus_tag="ACHUR4_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1457267.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(647..755)
                     /locus_tag="ACHUR4_003909"
                     /pseudo
     CDS             complement(647..755)
                     /locus_tag="ACHUR4_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1278..1354)
                     /locus_tag="ACHUR4_003910"
     tRNA            complement(1278..1354)
                     /locus_tag="ACHUR4_003910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1318..1320),aa:Arg,seq:tct)
     gene            complement(1456..1531)
                     /locus_tag="ACHUR4_003911"
     tRNA            complement(1456..1531)
                     /locus_tag="ACHUR4_003911"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1496..1498),aa:Ile,seq:cat)
     gene            complement(1676..2497)
                     /locus_tag="ACHUR4_003912"
     CDS             complement(1676..2497)
                     /locus_tag="ACHUR4_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1457268.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2512..2868)
                     /locus_tag="ACHUR4_003913"
     CDS             complement(2512..2868)
                     /locus_tag="ACHUR4_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1457269.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(2881..>3231)
                     /locus_tag="ACHUR4_003914"
     CDS             complement(2881..>3231)
                     /locus_tag="ACHUR4_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1457270.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          897 a          879 c          634 g          821 t
ORIGIN      
        1 tcaccagcaa ggcataaaca aggaaactaa atgaagtgga ttgtgattga tacagttatc
       61 cagccatcat gcggaatatc tttttcagtc atatggagta aaataaaatt aataatctgg
      121 tatcaatcgg atgctttctt acctcctgaa agtatattta cactgactca cacaggcatc
      181 atgctcaata acaaagtgct acctgtaacc atttacaacg tagtaccatt caataaaaca
      241 ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa cgtattgaat
      301 gaatgcttta ataaccgttg cactctgcaa atatgccctt atggactaaa acaacaaagt
      361 ccataaggag tttactcaca tctgacaaaa tcaatataaa cagcccctcc ggagaggggc
      421 tggagagtgg cgctatgtgc cattccatgg tgccgggtgc ctcccggtga attcagtacc
      481 agcacctgaa tccgcgatta tcccatatac ctactcgctg attgcccctc cgcacagggg
      541 gattcaccat gccagtttct tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc
      601 tgaattgtga agtatttaaa aatttctccc gctaactgat acccggctaa cagtctggcg
      661 ttttcttttt cagcaacggg aaagcaacaa ccaccacacc cgccaccagc acaccgtcag
      721 ccggcactga cattatccgg ctgctgcaat gccattaaca aaaacagtaa gcaatcactt
      781 tttaccgtaa caggtgataa tccagatatg tatctacccc agatgagtaa tccgaagttc
      841 atccatacca caggtcctgg ctattctgtt gtactcctga acaagagcaa ataattctga
      901 attagcaacc atgaactcat cgcaaaccct ctgtatagca tcactattca gaataataac
      961 gtctcttccc gaaagacgat caggagtaca gaacaaaact gtcaaacggc tgaaggcctt
     1021 tgctcgtgct gcattgacta tatcaatacg ctgcctaagg atgaaacacc ccgacgcctc
     1081 atcaatattc actctaccca caccatatga atgataaata cttaatgctg aaaaaaccat
     1141 tagaccgtat aacaaatact caatcaatac ttaacagaac ttttattttt gacaaatata
     1201 taatattttc aacaatatcc tgagccaggt atatttcagt ataaggctct gccggaagga
     1261 atctggaaga atgaatatgg cgcgctgtac tggattcgaa ccagtgaccg attgcttaga
     1321 aggcaattgc tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg
     1381 gggacccgcc ccccgcacca acaaccctgt tatcgtgtcg tctgctcttc ctgataagct
     1441 aatggcggtt tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
     1501 tcgctcgctc tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta
     1561 atttcgcaat aaaaaaaccc gctcagcggc gggttgtaga aactcttcta acgccaggca
     1621 taaaacgccc atcattatga cgaatttacc acagattccg gaaaaatcaa ccttgttacc
     1681 tagttacctt ttttaactgc cgctcagccc atgcttcttc aatatcaaac cgggtcacca
     1741 gcgcatcata aaatttctta actgtttttt cccatgacgc gcgtgttatc tggtttgtca
     1801 cctcgcatat agcattaaat gcctccgttg atggtagtct ttcatagcca cgaccaccac
     1861 aacgctggca gtctctgata acaggcatac cacgttttac cgactcttca cgatgaatgg
     1921 cgacaccacg cccacggcaa tccttacagg cggtggaaac ctcacccttt ccgccacact
     1981 ccggacaggc aacttttacc acctccctga cttttttcca ttcttcccag taaaacggat
     2041 acacaccttt cgtacacttt gcccataccg gcggcttacc atccggatac tggatcttgt
     2101 ttgtaaaaac tatgctttca ataaattttt ccccatagca acaagggcac tgctttttac
     2161 tcgctgcgct gcgggcataa tcctcaaaag cgtacgaagc cataatgcgc atcactaccg
     2221 gttttatttc tgccggaagt tttctcaacg ccgccacacg atcgcaccga ctgagtgcat
     2281 aatctgccag taattctgtt gcccgcgccc tgtcattcat actgatgccc attttcccca
     2341 ggaacgcaga aaaacccatc tcagcccgat tctgtgtcat gccctgcgcg gccatcacat
     2401 cagtgatact cagcgcatct tttgacgttg aggccgatgc atcggtcagg ccaggggatt
     2461 ttggggagta gtatttcggt aaatcttcca gtttcatttt ttgacctgct cttaatgcat
     2521 tatggggtaa atcttcaccc ccagacgtcc accagatact ggctgaccac gaacgatatt
     2581 gatttcatca aactgctcat cgtccattaa cactcccgca tgcgtcagcg catccagcgg
     2641 tgctttcagg atattgtcca ggtcgcgacg acgcttatcc ggtggctctg caatcacctt
     2701 tatcgccagc cttccggaca ggcttaattt cagccgctgc tggcgaacaa taagcgccac
     2761 agcccggcga taacgctttc cctcctccga gataaaatat gtgctgccac ggcgtcgcca
     2821 gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca gtcacctctt
     2881 ttacccaagc acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg
     2941 agaaccatgc ttttcttcga acgccagagg atccgcatga agctcgttgt gatgctcccg
     3001 acacagcggt agcgtgaaaa tatcgtgaga tttggtcccc attccgccct gaccatgacc
     3061 aatcaggtga tggggatcgt cggctggctt accacaacac gcacacggct gtgtcttcac
     3121 ccagcgtttg tatttctcgt taacccagcg gcgacgttta ggtcgtttca tgaaagattc
     3181 cggagactca ggatcaacgg caatgctgac caccgtcttt tcctgttgtg g
//
LOCUS       DAZPFP010000272         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPFP010000272 DAZPFP010000000
VERSION     DAZPFP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.277"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUR4_003915"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUR4_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1457271.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHUR4_003916"
     CDS             complement(1772..1906)
                     /locus_tag="ACHUR4_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1457272.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHUR4_003917"
     CDS             complement(2552..2722)
                     /locus_tag="ACHUR4_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1457273.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZPFP010000273         3178 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPFP010000273 DAZPFP010000000
VERSION     DAZPFP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.278"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(16..642)
                     /gene="dsbA"
                     /locus_tag="ACHUR4_003918"
     CDS             complement(16..642)
                     /gene="dsbA"
                     /locus_tag="ACHUR4_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1457274.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(659..1645)
                     /gene="srkA"
                     /locus_tag="ACHUR4_003919"
     CDS             complement(659..1645)
                     /gene="srkA"
                     /locus_tag="ACHUR4_003919"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1457275.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1722..1991)
                     /locus_tag="ACHUR4_003920"
     CDS             complement(1722..1991)
                     /locus_tag="ACHUR4_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1457276.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2061..2645
                     /gene="mobA"
                     /locus_tag="ACHUR4_003921"
     CDS             2061..2645
                     /gene="mobA"
                     /locus_tag="ACHUR4_003921"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1457277.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2627..3154
                     /gene="mobB"
                     /locus_tag="ACHUR4_003922"
     CDS             2627..3154
                     /gene="mobB"
                     /locus_tag="ACHUR4_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1457278.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          835 a          771 c          763 g          809 t
ORIGIN      
        1 gtaatttaca ttgaattatt tttcctcgga cagatatttc accgtatcag catactgctg
       61 aacaaaaaca tccatattgc tggtatccat accctgcgga ttcagctgat atttaccgtt
      121 aacaaacatc gccggaacac cacgcaattg cacatcagct gcggcttttt cctgctgagc
      181 gaccagagat ttcaccacga agctgttcca cgccgcgtcg tactcttcac ctttaatacc
      241 tgcattgata aatacatcgc ggatatcaga tgctgaacga atggtctggg ttttctgtac
      301 gccttcaaac agcggaacag tcactttgtc ttccacgccc agcgccatcg ccacagccca
      361 tgcctgagtc agatctttgc ccaggtcacc acccatgaag ttgacgtggt atttagtcat
      421 cttcacgcct tccggcagtt ttttcttcac attatcagaa atatgcagaa cttcttcaaa
      481 ctgatagcag tgcgggcaga agaaagagaa aaactccagc acttgcggcg cgccagctac
      541 cggtttttcc agggtagtgt actgtttacc atcttcatac tgcgccgccg atgcgctaaa
      601 cgctaaaact aaaccagcca gcgccagcca aatctttttc atgatctact ctctccgatt
      661 aatacatagg tgttaattgc aaagggggtt cttgtagaac ttttgcctgt tctataaaag
      721 tcgccgtctg tcgtagccag taatcttccc cggttaacca cgggaaattt ttcgggaacg
      781 cgggatcagc ccaatgccgc attagccagg caagataata aaccaaacgc atggcgcgta
      841 aaggttcaat cagtccgatt tcagcggtgt cgaactcgct aaattcttca taagcttcaa
      901 taatagtttc cagttgcatc cgctgctggg ctttatcgcc attgagcaac atccacaaat
      961 cctgaatagc tggaccatta cgtgcatcat ccagatcaac aaacattgga ccatcgcgcc
     1021 agagaatatt cccggcgtgg cagtctccat gtagccgcag aacggtgaaa tcttcccgcc
     1081 agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt cagaaatgcc gctttcaacc
     1141 cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg ctcaatgaga tattcattca
     1201 gaccgatggt cgggcgatga ataaaaagct gtttgcgccc cgtctggtgc atacgcccta
     1261 aataacgccc aaccgcttcc atctgatcga tattatcagc ttcgaactgg cgaccaccga
     1321 cgcttggaaa aacagcgaaa taaaatccct gatgattcaa taaagtctga ccgttaaagg
     1381 ccacaggtgc tgcgaccgga acttcatcat ttaccagctg caacgcaaat tgatgttctt
     1441 cgaggatttg atcggctgtc caacgttcag ggcgataaaa tttgacgaca aaacgtcgac
     1501 gctcttcgtc ctgaaattga tagacacggt tttcatagct gttaagcggg gtaagaccgg
     1561 aatccacccg gatcccttgc ttaaacagag cgtccatgat ggtatccggg tgtagtgtct
     1621 ggaaagtaaa agcgctgttg ttcatcccat catccggaaa acattacgaa tgatgaagga
     1681 tatcatttca cgatgatttt ggtggcgccg cttacaagct tttactcttt aattacgccg
     1741 cgagcgcgta acagcgccgt tttgaagtct tcttcataat ctttctgcaa acccgggatc
     1801 accgcatctt tcgcggaatc acgcattttc aggtgataga tcagaatgtc atccgtcaaa
     1861 tccgccagtt cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa ttgcaacaag
     1921 ttaaagtctg gctctttttg ccaggctggc tggaggagtt caataacttc attcagacgt
     1981 ttacatttca tagtaatgct ccttactctt gagacagaca cgttagcagg gtcaatccca
     2041 caataaaaga ggcgatatcg gtgaatctaa tgacgacgat aacaggcgtt gtgctggcag
     2101 gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt gctggactta aatggcaaac
     2161 cgctgtggca acatgtcgct gacgcgctta agacgcagct ctctcacgtc gtggttaatg
     2221 ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa gattcactgg
     2281 cggattaccc aggccctctg gcaggaatgc tttcagtaat gcagcaggaa gctggtgagt
     2341 ggtttttgtt ttgtccgtgc gatacgcctt acattcccca tgatttagcc gcccggctta
     2401 atcatcagcg caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc gatcacccga
     2461 ctattgctct ggtaaaccgc gctattgagc ctttattact ggaatatctg caagcaggag
     2521 aacgccgggt aatggcattt atgcgtctgg ctggcggtca tgcggttgat ttcagcgatc
     2581 ataaagatgc atttgttaac gtgaatacgc cagaggagct tgcccgatgg caggaaaaac
     2641 gatgataccg ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta cgcttttgaa
     2701 aaaactgatc ccggcattat gcgccagagg gatccgtccg gggctgatta agcatacgca
     2761 ccatgatatg gatgttgata agccaggcaa agatagctat gagctgcgca aggctggcgc
     2821 ggtacaaacc atcgttgcca gccagcaacg atgggccttg atgacggaaa caccagacga
     2881 agaagagctg gatctacatt ttctcgcaag tcgaatggat acctcaaagc tggatttgat
     2941 tctggtcgaa gggtttaagc atgaagagat cgcaaagatt gtgctgtttc gcgatggagc
     3001 cggacatcga cctgaagaat tagtgataga caggcatgtt attgctgtag ccagtgatgt
     3061 gccgcttaat cttgatgtcg cattactgga tattaatgat gttgaggggc tggctgattt
     3121 tgtggtggag tggatgcaaa agcagaacgg ataagcaaga gtaggctgga tcagacgc
//
LOCUS       DAZPFP010000274         3114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPFP010000274 DAZPFP010000000
VERSION     DAZPFP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.279"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            95..2248
                     /locus_tag="ACHUR4_003923"
     CDS             95..2248
                     /locus_tag="ACHUR4_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1457279.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>3114
                     /locus_tag="ACHUR4_003924"
     CDS             2301..>3114
                     /locus_tag="ACHUR4_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457280.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYL"
BASE COUNT         1133 a          563 c          572 g          846 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaa
//
LOCUS       DAZPFP010000275         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPFP010000275 DAZPFP010000000
VERSION     DAZPFP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.280"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHUR4_003925"
     CDS             <1..498
                     /locus_tag="ACHUR4_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457281.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHUR4_003926"
     CDS             578..1273
                     /locus_tag="ACHUR4_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1457282.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHUR4_003927"
     CDS             1302..>3069
                     /locus_tag="ACHUR4_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1457283.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZPFP010000276         3067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPFP010000276 DAZPFP010000000
VERSION     DAZPFP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.281"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUR4_003928"
     CDS             <1..224
                     /locus_tag="ACHUR4_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1457284.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHUR4_003929"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHUR4_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1457285.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHUR4_003930"
     CDS             518..1108
                     /locus_tag="ACHUR4_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1457286.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHUR4_003931"
     CDS             1348..1608
                     /locus_tag="ACHUR4_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1457287.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHUR4_003932"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHUR4_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1457288.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHUR4_003933"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHUR4_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1457289.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          828 a          724 c          781 g          734 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattac
//
LOCUS       DAZPFP010000277         3066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPFP010000277 DAZPFP010000000
VERSION     DAZPFP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.282"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUR4_003934"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUR4_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1457290.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHUR4_003935"
     CDS             348..641
                     /locus_tag="ACHUR4_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1457291.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHUR4_003936"
     CDS             713..1372
                     /locus_tag="ACHUR4_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1457292.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHUR4_003937"
     CDS             1464..1910
                     /locus_tag="ACHUR4_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1457293.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUR4_003938"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUR4_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          804 a          659 c          725 g          878 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gtttta
//
LOCUS       DAZPFP010000278         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPFP010000278 DAZPFP010000000
VERSION     DAZPFP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.283"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHUR4_003939"
     rRNA            <1..2850
                     /locus_tag="ACHUR4_003939"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2946..>3046
                     /gene="rrf"
                     /locus_tag="ACHUR4_003940"
     rRNA            2946..>3046
                     /gene="rrf"
                     /locus_tag="ACHUR4_003940"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          802 a          673 c          950 g          621 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     3001 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagt
//
LOCUS       DAZPFP010000279         3024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPFP010000279 DAZPFP010000000
VERSION     DAZPFP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.284"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            51..974
                     /locus_tag="ACHUR4_003941"
     CDS             51..974
                     /locus_tag="ACHUR4_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1457294.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(1012..2652)
                     /gene="trg"
                     /locus_tag="ACHUR4_003942"
     CDS             complement(1012..2652)
                     /gene="trg"
                     /locus_tag="ACHUR4_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1457295.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT          729 a          786 c          710 g          799 t
ORIGIN      
        1 atcaaacaac ttcacttgtc agcgacaccg cttcgttttt aacatcgctt atggaaaaaa
       61 atagtctgtt tagtcagcgc atccgtttgc gccaccttca tacattcgta gctgtcgcac
      121 aacaaggaac tttggggcgc gcagctgaaa cccttaattt gagtcaacct gcgctctcta
      181 atacattgaa tgaactggag cagctgacgg gcgctcgctt gtttgagcgt ggtcgtcagg
      241 gggcgcaact taccttaccc ggcgaacaat ttttaacgca tgcagtcaga gttcttgacg
      301 ccatcaacac tgccggacag tcgcttcatc gtaaagaagg tcttaataat gatgtcgtca
      361 gggttggtgc actacctact gcggcactgg ggatattacc ttcggttata ggtcagtttc
      421 atcagcaaca aaaagagacg accttgcaag ttgcgacaat gagtaaccct atgattctgg
      481 cgggtttaaa aaccggggaa atcgatatcg gcattggtcg gatgtcagat cctgaactga
      541 tgaccgggct taattacgaa ctgctgtttc ttgaatcgtt gaagctggtt gtccgcccta
      601 atcacccgct acttcaggag aacgtaacgc taagccgggt gctggaatgg ccggtcgttg
      661 tatcaccaga aggcactgcg ccacgccagc attcagatgc attagtgcag agccagggct
      721 gtaaaattcc ttcgggttgt atcgaaacgc tgtctacttc gctatctcgt caacttaccg
      781 ttgaatacga ttacgtgtgg tttgtccctt ctggcgctgt aaaagacgac ctgcgtcatg
      841 ccacgctggt ggccctgcct gttccgggac atggtgcagg cgaaccgatt ggaatactga
      901 cccgcgtaga tgcgacgttc tcttctggtt gccagttgat gattaacgct attcgaaaat
      961 caatgccgtt ctgaaaggtg aagggatctg tcgatccctc cttgaacatt ttcacaccgt
     1021 agcgaaacta actggttcac ccgctccgcg aggttctgcc gacacagaat gtttgttcag
     1081 acggaatacg ttcaccgcct cagttaatcg tgcagcctgt tcttcaaggg acaccgccgc
     1141 tgctgaggcc tcttctacca gagaagcatt ctgttgcgtc accttatcca tttcagaaat
     1201 cgcctggcta acctgagtta tgcctctact ttgttcatcc gaggcggcgg cgatttcctg
     1261 catgatatgt gtgacactcg cgacggcatc aacaatagtg ctcatggttt tccctgccgt
     1321 tgccacctca tccgacccca ggtcaattaa cctgactgat tcactgatca agccttcaat
     1381 ctctttcgct gcctgagcgc tgcggcttgc gagtgtccgt acttcgctgg cgacaacggc
     1441 aaatccacgc ccttgctcac ccgctcgcgc ggcttcaacg gcagcattca gtgccagaat
     1501 attcgtctgg aaagcaatac tgttgatgac ggcggtgatc tcagaaattt tcttcgaact
     1561 cgtggagata gcgcccatcg tttttactac accggaaacc gtctgcccgc catcgctggc
     1621 tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc gcgttatccg cattctgttt
     1681 caccgtcgca gtgagttgct ccatgctggc ggcagtttgt tcgatagccg ccgcttgttc
     1741 ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt tcgctggtgc cacgataaat
     1801 ctcttccgcg ccctgtcgaa cagtccctac tgtcatcccc agtgaatgct gcatttgctg
     1861 taaatgacga cttaagcgac cgatttcatt acgacccgcc ggttcatcat tcatcgtcag
     1921 atcgccactg gcgatttttt caatccgttg tgcggcatgt tgcagtggac gaatgacgat
     1981 ccgacgtagc accataaatg ttatcagcgt catgaccagg gcaagcacaa acgcgccaat
     2041 catgaacatc ccacccaggc gggtgcgctg atgggccagt tccgccagtt ggttggctct
     2101 ggtgctacgt atcttaacgg ctttgttcaa aatatcggta taagcattat ccagcggtcg
     2161 gatctgctca ctttcatgat tgataatcgc ttcaaacatg ccatttttgg catatttcaa
     2221 cataggttgc atacccgtga tataagcctg aaagcgttga tttaattcag tgtcgaaggc
     2281 ttcatcagca ggtgttttca ccggtcgatt ctgataagca cgataacctt gctgcgactg
     2341 tttaatctcc gattcggctt gcgcaatatt tcgcttcatt gcttccattt ccgcaatacg
     2401 actcgcagcc ccggcctgaa tcatattgat ccgggctgaa cgcaaaaaat ctgaactgtt
     2461 cgctaacccg gtccgaatct caatctccgc agtaacatca cgctgatcgc gatcggcctg
     2521 ccagaggaaa tagccagcca gagcagaact taatgcgaat agaactaaga taccgccaag
     2581 aatgcaggca aataacggaa ccagcctgat gtgatgcaaa aaacctaatc gctgtgaggg
     2641 agttgtattc attaccgttg tctctcgtcc aggtttactc ctgatagaaa gtcatcggca
     2701 cttgacggta attacttatg accaaaaacc tctttgcgtc acatttttac aacaattatt
     2761 gcagagaaaa ttaatgctat tggaatgaag agagtaacga atggctaaac tggctaaccc
     2821 gaatgacgaa aaattctgga aaacagcacg caatttcgtg aagtgattat tttcgtccct
     2881 aaggaaacct cataggatgc ctgcgggtgc ctgagacttt ctgttttcag gctaaccggg
     2941 aatcagaaag acgaaggccc cgaccgatat ttctatcaac tgtactgcac ccattttgtt
     3001 ggacgatgaa atggaatagc ccct
//
LOCUS       DAZPFP010000280         2994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPFP010000280 DAZPFP010000000
VERSION     DAZPFP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.285"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1031
                     /gene="tsuA"
                     /locus_tag="ACHUR4_003943"
     CDS             <1..1031
                     /gene="tsuA"
                     /locus_tag="ACHUR4_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1457296.1"
                     /translation="CGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISAQSV
                     GVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIA
                     LFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMK
                     ELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLG
                     VTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRAADAQTTLRSG
                     LGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKA
                     RLATAAAN"
     gene            1045..1272
                     /gene="tsuB"
                     /locus_tag="ACHUR4_003944"
     CDS             1045..1272
                     /gene="tsuB"
                     /locus_tag="ACHUR4_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1457297.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1315..2739)
                     /gene="sbcB"
                     /locus_tag="ACHUR4_003945"
     CDS             complement(1315..2739)
                     /gene="sbcB"
                     /locus_tag="ACHUR4_003945"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1457298.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2921..>2994
                     /locus_tag="ACHUR4_003946"
     CDS             2921..>2994
                     /locus_tag="ACHUR4_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457299.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADA"
BASE COUNT          691 a          698 c          791 g          814 t
ORIGIN      
        1 tttgtggtgc tctgctggga tttgtcatgc agcgtgggcg tttttgcctg actggtggtt
       61 ttcgcgatat gtatatcgcg aaaaataatc gcatgtttta cgccttgctg attgcaattt
      121 cggcacaaag cgtgggggtt tttgcgttaa ttcaggcggg cctactgact tacgaagccg
      181 gggcgttccc gtggcttggt actgttatag gtgggtatct tttcgggctg ggaattgttc
      241 tggcgggtgg atgtgccacc gggacctggt atcgcgcggg tgagggattg atcggcagtt
      301 ggatcgcgct tttcacttat atggtgatga gtgcggtgat gcgttctcca cacgccagtg
      361 gtttaaatca aaccttgcag cactacacta ctgaacataa ctctattgct gagactttta
      421 atttgtctgt gtggccgttg gttaccgttt tgctggtgat aacgctctgg gtggtgatga
      481 aagagttgaa gaagccaaaa ctcaaagtcg cgaccttacc gccgcgccga accgggatcg
      541 ctcatattct gtttgaaaaa cgctggcatc cctttgtcac tgctgtactc atcggtttga
      601 ttgcgctttt agcctggccc ctgagtgaag caaccggacg catgtttggg ttaggagtca
      661 cttccccaac ggccaatatt cttcaatttc tggtcgcggg tgacgtgaaa tacattaact
      721 ggggcgtttt cctggtgtta gggatcttcg tggaggcatt tattgcagcc aaagcgagcc
      781 gtgagttccg cgttcgcgca gctgatgcac aaacaacatt acgtagcggg ttaggtggtg
      841 tactgatggg cttcggtgcc agtattgcag gtggttgctc tatcggtaat ggactggtta
      901 tgactgcaat gatgacctgg cagggctgga ttggccttgt atttatgatt ctcggagtct
      961 ggactgcgtc ctggcttgtg tatgttcgac cgcagcgtaa ggcgcgactg gcaactgctg
     1021 cggcaaatta agtaaggtgt aaagatggcg attaaaaagc tggatgtggt gacgcaggtc
     1081 tgtccgtttc cactcataga agcaaaagca gcactggcag aaatggccag cggcgatgag
     1141 ttggtaattg agtttgattg cacccaggca actgaagcaa tcccacagtg ggcggcagaa
     1201 gaaggacatg ccatcaccga ttatcagcag attggcgatg cagcgtggag catcaccgtt
     1261 caaaaagcct gactcaacat tgtcctccgc cgtaccagcg gcggcggctt caaattagac
     1321 gatctcttcc gcgtactgcc aaagtgcttt taacagcgcc actttctctt tgtcatcggc
     1381 atattgttgt accagcatct gcaattcatc agcataaccc tgcaaaaact ctggcgtgaa
     1441 gacctggtgg cggtgctcca gccagcgttg ctgctcggca taatccagcg tccccgggaa
     1501 gttgcgtgcc cgataattga acaacaactt ttcaattcgt ttatcaacaa aagtgatatc
     1561 cagtgccggt aaattacgcg gctcggtttc cagcacaatt ttcattgctg cacgatcggc
     1621 gtcactgaaa aagccattat aaagctgtgc atccacatta tctgaaggtg taaacggttc
     1681 ggcttccgcg aatatcgcta ccactttttc acgcacttgc ggattttcac gcagaatttt
     1741 taggttatcg aggcaatgct gacgattaat tcccagtcgg tcggcatctt ccgggcgtag
     1801 cgtattcgcc tgggccagta ccggacattt attgatatgc accagcttaa ccggaacggc
     1861 ggcgttatcg ccaagatcgg cttttgcggt atataaacgc tcgcgcaatg tgtcgatatc
     1921 cagctccagt aatggcgaaa tgtctcctgc caaatccgcc ataattacgg cattgcgatt
     1981 ttcaggatgc cacgccagcg gtgccaccca gctggtattg ccacgccatg ccccaaacat
     2041 tccggaaacg tgagccaggg gtttcatctg cggaacatca atcaacgcca tcagtttgtg
     2101 tttattacga tgggtaaaga gataatcaaa caggcgtggc tgacgcgttt ttaccagttt
     2161 cgccatcgca atagtggcgt acacatcagc catcgcatcg tgggcgttgc tatgttcaat
     2221 accattcgct ttggttaaat gctcaaggcg aaagctcggt agaccgtcat cattttcagg
     2281 ccagtttatt ccttccgggc gcagggcata acaggcacgc ataacatcca gtaaatccca
     2341 gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg tagaaattac gataaaaaac
     2401 gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag cccagaatac aggtcttcgg
     2461 tacggtaaaa agcgagtgaa tacgggcggc aaacgcggct tcgttttctc ctttcgcccg
     2521 tgcctcctgc ggggtaatac cggtaattaa tactgctcca ggccggggta aatagtcatc
     2581 cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca ttgaattcgt catcggtgcg
     2641 aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc gtgccaaagg tttcgtaatc
     2701 gtgaaagagg aaggtaggtt gtttatctgt gtctgtcatt gtctagcatt gtccactgta
     2761 gttaattaac tgtctgttat aactcgaaaa gcaacccttt tttcacctga gtatgtctac
     2821 gaacgtcaaa tatagttgtt tactgtgcgc tgttgtccat gtgatattgt gtaccagatg
     2881 tgtgccaaac tattatttct gagcgtacca aatattattt atggctataa gcgacacaaa
     2941 actgcgtact atttatggta aaccatattc gggcccacaa gaagtggctg atgc
//
LOCUS       DAZPFP010000281         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPFP010000281 DAZPFP010000000
VERSION     DAZPFP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.286"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHUR4_003947"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHUR4_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1457300.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUR4_003948"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUR4_003948"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1457301.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHUR4_003949"
     CDS             2603..>2951
                     /locus_tag="ACHUR4_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1457302.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZPFP010000282         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPFP010000282 DAZPFP010000000
VERSION     DAZPFP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.287"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHUR4_003950"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHUR4_003950"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1457303.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUR4_003951"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUR4_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1457304.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUR4_003952"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUR4_003952"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR1457305.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHUR4_003953"
     CDS             2756..>2932
                     /locus_tag="ACHUR4_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457306.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZPFP010000283         2906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPFP010000283 DAZPFP010000000
VERSION     DAZPFP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.288"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            139..771
                     /gene="comR"
                     /locus_tag="ACHUR4_003954"
     CDS             139..771
                     /gene="comR"
                     /locus_tag="ACHUR4_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1457307.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(833..1372)
                     /locus_tag="ACHUR4_003955"
     CDS             complement(833..1372)
                     /locus_tag="ACHUR4_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1457308.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1599..2903)
                     /gene="ndh"
                     /locus_tag="ACHUR4_003956"
     CDS             complement(1599..2903)
                     /gene="ndh"
                     /locus_tag="ACHUR4_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1457309.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          714 a          780 c          702 g          710 t
ORIGIN      
        1 acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc aattattttt
       61 atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc
      121 accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc
      181 cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa
      241 cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc caaagcgccc
      301 acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat
      361 atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag
      421 tctgcgctgg ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg
      481 gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg agatatagcc
      541 aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa
      601 cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt
      661 aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg
      721 atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa
      781 aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt
      841 tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt
      901 ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt
      961 tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt
     1021 cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag
     1081 tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca ccagcaacag
     1141 cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg
     1201 tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa
     1261 caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc
     1321 ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac
     1381 ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga
     1441 agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc tgaaggaatg
     1501 cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg
     1561 gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga
     1621 cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc
     1681 agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca
     1741 atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga gaagttcgac
     1801 agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt accgttcatc
     1861 tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg agcacgcggc
     1921 ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct
     1981 ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg gttagtttca
     2041 agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca taccatcaga
     2101 tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc actggtgacc
     2161 atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag
     2221 atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg
     2281 gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc gttgtgcaat
     2341 tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttgcca
     2401 tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg gtggaagcga
     2461 cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg cgtattgaaa
     2521 tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt acgttccgga
     2581 accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt cgcttcacga
     2641 tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc atgggccaga
     2701 tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagtggt
     2761 ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttacgtccc
     2821 agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca
     2881 atctttttca atggcgtagt caacgt
//
LOCUS       DAZPFP010000284         2906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPFP010000284 DAZPFP010000000
VERSION     DAZPFP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.289"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHUR4_003957"
     CDS             <1..1541
                     /locus_tag="ACHUR4_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1457310.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHUR4_003958"
     CDS             1593..2750
                     /locus_tag="ACHUR4_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1457311.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHUR4_003959"
     CDS             complement(2716..2838)
                     /locus_tag="ACHUR4_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457312.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          883 a          632 c          586 g          805 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgatt
//
LOCUS       DAZPFP010000285         2898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPFP010000285 DAZPFP010000000
VERSION     DAZPFP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.290"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUR4_003960"
     CDS             complement(<1..312)
                     /locus_tag="ACHUR4_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1457313.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUR4_003961"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUR4_003961"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1457314.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUR4_003962"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUR4_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1457315.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZPFP010000286         2889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPFP010000286 DAZPFP010000000
VERSION     DAZPFP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.291"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUR4_003963"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUR4_003963"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR1457316.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUR4_003964"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUR4_003964"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR1457317.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHUR4_003965"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHUR4_003965"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR1457318.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHUR4_003966"
     CDS             complement(1895..2809)
                     /locus_tag="ACHUR4_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR1457319.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAZPFP010000287         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPFP010000287 DAZPFP010000000
VERSION     DAZPFP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.292"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUR4_003967"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUR4_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1457320.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUR4_003968"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUR4_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1457321.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUR4_003969"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUR4_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR1457322.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZPFP010000288         2861 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPFP010000288 DAZPFP010000000
VERSION     DAZPFP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.293"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUR4_003970"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUR4_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1457323.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHUR4_003971"
     tRNA            complement(621..695)
                     /locus_tag="ACHUR4_003971"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHUR4_003972"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHUR4_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUR4_003973"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUR4_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1457324.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          691 a          697 c          689 g          784 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa a
//
LOCUS       DAZPFP010000289         2837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPFP010000289 DAZPFP010000000
VERSION     DAZPFP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.294"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHUR4_003974"
     CDS             complement(<1..593)
                     /locus_tag="ACHUR4_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1457325.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUR4_003975"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUR4_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1457326.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHUR4_003976"
     CDS             complement(1422..1961)
                     /locus_tag="ACHUR4_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457327.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUR4_003977"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUR4_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1457328.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          735 a          706 c          623 g          773 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgc
//
LOCUS       DAZPFP010000290         2819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPFP010000290 DAZPFP010000000
VERSION     DAZPFP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.295"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            19..573
                     /locus_tag="ACHUR4_003978"
     CDS             19..573
                     /locus_tag="ACHUR4_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1457329.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="ACHUR4_003979"
     CDS             complement(583..2010)
                     /locus_tag="ACHUR4_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1457330.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /locus_tag="ACHUR4_003980"
     CDS             complement(2007..2714)
                     /locus_tag="ACHUR4_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1457331.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          814 a          671 c          618 g          716 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgatgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      361 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      601 tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      661 acagcccagc ccgccatcat caacgggcat tggctgcggt tgcggctccg gctcgacggt
      721 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
      841 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccacta atgggtaata atagacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag
     1201 caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag
     1321 gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaactggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg
     2041 catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2281 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2341 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca
     2461 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttct cgatcattcg gtgaattatg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc
     2761 gaggctcagt tatttatact tttctgacgc aacataatac gcataaaata aggcttacg
//
LOCUS       DAZPFP010000291         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPFP010000291 DAZPFP010000000
VERSION     DAZPFP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.296"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUR4_003981"
     tRNA            6..81
                     /locus_tag="ACHUR4_003981"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUR4_003982"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUR4_003982"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUR4_003983"
     CDS             complement(299..1057)
                     /locus_tag="ACHUR4_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1457332.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUR4_003984"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUR4_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1457333.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHUR4_003985"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHUR4_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR1457334.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZPFP010000292         2752 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPFP010000292 DAZPFP010000000
VERSION     DAZPFP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.297"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(178..1602)
                     /gene="patD"
                     /locus_tag="ACHUR4_003986"
     CDS             complement(178..1602)
                     /gene="patD"
                     /locus_tag="ACHUR4_003986"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1457335.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1624..2418)
                     /locus_tag="ACHUR4_003987"
     CDS             complement(1624..2418)
                     /locus_tag="ACHUR4_003987"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1457336.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2408..>2752)
                     /locus_tag="ACHUR4_003988"
     CDS             complement(2408..>2752)
                     /locus_tag="ACHUR4_003988"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1457337.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          708 a          811 c          642 g          591 t
ORIGIN      
        1 aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca
       61 aacatttagc ctaattttta cgccagttat taatgtaatc attctaatac ttaaccgcca
      121 ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta
      181 atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc
      241 cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg
      301 ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac
      361 atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac
      421 ctgttcttcg ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcac
      481 gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc
      541 cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac
      601 tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc
      661 gtcatccggc gcaccagatt ttaacgttgc caccgcagca cccagttttt ccaccagcgt
      721 atcgtaaatg cctttttgcg cgtagatccg acaagccgca gtacaatcct gtccagcatt
      781 gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat catcaaaaac
      841 aatcactggc gctttgccac caagttccat atgagtacgc ttaatggacg acgcggtatg
      901 gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc gcactttggg
      961 atgaccggtc agcggatcac ccaccgtttt gcctctgcca aacagtatgt taatcacgcc
     1021 tgccgggaag atatctttcg ccagctctgc caacttcaac gcggtcagcg gggtaatttc
     1081 tgatggttta agcactacgc agttccctgc cgccagcgcc ggagcaagtt tccacgcggc
     1141 catcatcagc ggataattcc acggtgcgat agaagccacg acccccaacg gatcgcgacg
     1201 gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca gacagcgcgc
     1261 cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg atttcatcat tgaacgcact
     1321 atgcagcggt ttgccacaat tacgggattc cagttcggca aaaacctgac cattttcttc
     1381 gataacatcc gccagtttca gcaggcattc cgcacgcgct ttcggtgtgg tttgccccca
     1441 ttcagcaaat gctgcatctg ccgcacgcac agcagcatcg acctgctctg cggatacctc
     1501 ggcaatctcc agtaaaacgt cccccgttgc cggattatag acaggctgtt tttccccttc
     1561 gccgctaacc cgttctccgt taatcagtaa cttatgttgc atagcttatt cctgtatcag
     1621 tggttattga ccattgtcgc cttcgcgggt tagccaccag gcccccagga tcggcaaggt
     1681 tgttaccaac ataaccagca gtgccaccac gttggttacc ggtacatcac gcggtcgccc
     1741 aagctgattg agcaaccaca acggtaacgt tcgttcatga cctgccgtaa aggtcgtaac
     1801 gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta acgccgaact
     1861 gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa gatccattga
     1921 cgcctcaacc agactccacg aggtgcggcg aaaacgggcg atgacattgt taaacaccac
     1981 cactacgcaa aaagtcgcat gaccgaccac gatggtgaaa aatcccggct ccagattgat
     2041 ggttttaaag gcggttaata gcgccagacc agtgacaatg cccggcagcg caatgggcag
     2101 cagcaataac agcgaaatgg cgtttttgcc gaaaaagtct cgtctccaca gcgcggcagc
     2161 tgccagcgtc cctaacacca gcgcaattaa tgtcgccagc gccgccactt taagtgacag
     2221 cgtcacggca tcaagaatat cactacgctg tgctgccacg ctaaaccagc gcagcgtcag
     2281 gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcatagg cggcgattat
     2341 caggatggga aaatgtagga aaacaacgcc gccccaggcc gccagtttga ggaaaaacgg
     2401 tgcgcgttca gagtgcatcg aacgctccca gacgtttcac gaacgccagg tacagtgcga
     2461 tgagaataat cggcaccagg gtgaatgccg ccgccatcgg catattgcca atcgccccct
     2521 gctgggaata aaccatattg ccgataaaat atcctggagg accaaccagc tgcgggacga
     2581 taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc agcggcaata cccgggattg
     2641 ccagcggcag caccacatag cgaaaggttt gtcgtggacg tgcgccgaga tcagccgacg
     2701 cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg aacgggcagg at
//
LOCUS       DAZPFP010000293         2749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPFP010000293 DAZPFP010000000
VERSION     DAZPFP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.298"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHUR4_003989"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHUR4_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHUR4_003990"
     CDS             381..629
                     /locus_tag="ACHUR4_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1457338.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACHUR4_003991"
     CDS             complement(745..1014)
                     /locus_tag="ACHUR4_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1457339.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACHUR4_003992"
     CDS             1071..1739
                     /locus_tag="ACHUR4_003992"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1457340.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2749
                     /locus_tag="ACHUR4_003993"
     CDS             2055..>2749
                     /locus_tag="ACHUR4_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457341.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          842 a          596 c          539 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgttacaaca aaccgccca
//
LOCUS       DAZPFP010000294         2746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPFP010000294 DAZPFP010000000
VERSION     DAZPFP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.299"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            110..1084
                     /gene="cysB"
                     /locus_tag="ACHUR4_003994"
     CDS             110..1084
                     /gene="cysB"
                     /locus_tag="ACHUR4_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1457342.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1403..1543
                     /locus_tag="ACHUR4_003995"
     CDS             1403..1543
                     /locus_tag="ACHUR4_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1457343.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1546..1713
                     /gene="yciX"
                     /locus_tag="ACHUR4_003996"
     CDS             1546..1713
                     /gene="yciX"
                     /locus_tag="ACHUR4_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1457344.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1827..1922
                     /gene="ymiC"
                     /locus_tag="ACHUR4_003997"
     CDS             1827..1922
                     /gene="ymiC"
                     /locus_tag="ACHUR4_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1457345.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2086..>2746
                     /locus_tag="ACHUR4_003998"
     CDS             2086..>2746
                     /locus_tag="ACHUR4_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR1457346.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT          712 a          636 c          645 g          753 t
ORIGIN      
        1 tgatatagtg gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag
       61 gtgtgacgaa aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca
      121 acaacttcgc tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga
      181 aggactttac acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct
      241 aggcattcaa attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcaggaca
      301 agaaataatt cgtatcgctc gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc
      361 cggtgagcac acctggccgg ataaaggctc gctgtatatc gccaccacgc atacccaggc
      421 acgctacgcg ttaccaaacg ttatcaaagg ctttattgag cgttatcccc gcgtttcttt
      481 gcatatgcac cagggcccgc cgacacaaat tgctgatgcc gtctctaaag gcaatgccga
      541 tttcgctatc gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta
      601 ccactggaat cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat
      661 taccattgaa gaactggcgc aatatccgtt ggtgacatat accttcggct ttaccggacg
      721 ttcagaactg gatactgcct ttaaccgcgc agggttaacg ccgcgtatcg ttttcacggc
      781 aacggatgct gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc
      841 cagcatggcg gtggatccgg tcgccgatcc cgaccttgtg cgcgttgatg ctcacgatat
      901 cttcagccac agtacaacca aaattggttt tcgccgtagt acttttttgc gcagttatat
      961 gtatgatttc attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt
     1021 cgcattgcgc tccaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa
     1081 ataatttctt gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc
     1141 ttttgttact tatttctata ccgtgcacaa tatttttatc cctcatattt atagggtaaa
     1201 ttacctgtaa agtagtgtct aattacccgc ctcgcaaatt ccagttttca aatatctatt
     1261 tcaggtcaaa agattgaatt attcactgga gacgattcgt aaattcgctg gatttttgac
     1321 taaagtttct ttaggatttg tctcatcgta tgattaatta caccaatcat tcaacgtctt
     1381 gctgataagt gatggcgatt atatgaggtt agcaatgcct tctggaaatc aggaaccccg
     1441 ccgtgatcct gaattaaaac gtaaagcctg gctggcggtt tttcttggtt ctgcactttt
     1501 ctgggtggtt gtcgcactgc tgatttggaa agtgtgggga taactatggt cggtcaggag
     1561 caactggagt cgtcaccatt atgccagcat agtgacaatg aaccagaaac gaaacgggaa
     1621 tgttccgtcg ttattccaga cgactggcaa ctaacatcgc agcagcaagc ctttatagaa
     1681 ctgtttgctg aagatgatca gccgaaacaa taattatcat cattcttatt acccattttt
     1741 aatgaattaa agggctttta atacaccgca gcaataacag cttgagttat ctcaacacaa
     1801 aataataacc gttaagggtg tagcctatga tcaacacaaa tatgaaatat tggtcctgga
     1861 tgggcgcgtt ttctctgtcg atgctcttct gggccgaact cctctggatc attactcact
     1921 gatccttgac cccgctgcgg cggggttgtc atttgctttg ccacaaggtt tctcctcttt
     1981 tatcaatttg ggttgttatc aaatcgttac gcgatgtttg tgttatcttt aatattcacc
     2041 ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc gtcaacccta
     2101 cgagaagcca gtaaggacac gttgcaggcc aaagataaaa cttaccacta ctacagcctg
     2161 ccgcttgctg ctaaatcact gggcgatatc acccgtctac ccaagtcact caaagttttg
     2221 ctcgaaaacc tgctgcgctg gcaggatggt aactcagtta ccgaagagga tatccacgca
     2281 ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg
     2341 gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc aatgcgcgaa
     2401 gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc ggtcgacctg
     2461 gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac
     2521 gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg gggaaagcaa
     2581 gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca tttgccatca ggttaacctc
     2641 gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat tgcttatccg
     2701 gatacactcg ttggtactga ctcgcacacc actatgatca acggcc
//
LOCUS       DAZPFP010000295         2716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPFP010000295 DAZPFP010000000
VERSION     DAZPFP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.300"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            54..2576
                     /gene="lpfC-O113"
                     /locus_tag="ACHUR4_003999"
     CDS             54..2576
                     /gene="lpfC-O113"
                     /locus_tag="ACHUR4_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1457347.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2587..>2716
                     /locus_tag="ACHUR4_004000"
     CDS             2587..>2716
                     /locus_tag="ACHUR4_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457348.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          679 g          724 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccctcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccag gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg
     2281 cagtggttaa agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa
     2341 atgcaaatgg taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg
     2401 cagattcagg aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag
     2461 gacaggttac gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcat
     2521 tttcgacacc agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg
     2581 atttttatga acaagtatat caaacagtgg tgctttgctg tgtttatgct ctcgttaagt
     2641 agtgtagcac ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt
     2701 acgcttgatt tctcgg
//
LOCUS       DAZPFP010000296         2708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPFP010000296 DAZPFP010000000
VERSION     DAZPFP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.301"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            184..1350
                     /gene="nhaA"
                     /locus_tag="ACHUR4_004001"
     CDS             184..1350
                     /gene="nhaA"
                     /locus_tag="ACHUR4_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1457349.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1416..2315
                     /gene="nhaR"
                     /locus_tag="ACHUR4_004002"
     CDS             1416..2315
                     /gene="nhaR"
                     /locus_tag="ACHUR4_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1457350.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2353..>2708)
                     /locus_tag="ACHUR4_004003"
     CDS             complement(2353..>2708)
                     /locus_tag="ACHUR4_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1457351.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          626 a          599 c          696 g          787 t
ORIGIN      
        1 tctgggctta gggtcactcg tgagcgctta cagccgtcaa aaacgcatct caccgctgat
       61 ggcgcaaatt cttcaatggc tcgtaaaaaa cgaattattc ctacactata atctgatttt
      121 aacgatgatt cgtgcggggt aaaatagtaa aaacgatcta ttcacctgaa agagaaataa
      181 aaagtgaaac atctgcatcg attctttagc agtgatgcct cgggaggcat tattcttatc
      241 attgccgcta tcctggcgat gattatggcc aacagcggcg caaccagtgg atggtatcac
      301 gactttctgg agacgccggt tcagctccgg gttggttcac tcgaaatcaa caaaaacatg
      361 ctgttatgga taaatgatgc gctgatggcg gtatttttcc tgttagtcgg tctggaagtt
      421 aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc aggccgcatt tccagttatc
      481 gccgctattg gtgggatgat tgtgccggca ttactctatc tggcttttaa ctatgccgat
      541 ccgattaccc gcgaagggtg ggcgatcccg gcggctactg acattgcttt tgcacttggt
      601 gtactggcgc tgttgggaag tcgtgttccg ttagtgctga agatcttttt gatggctctg
      661 gctattatcg acgatcttgg ggccatcatt atcatcgcat tgttctacac taatgactta
      721 tcgatggcct cccttggcgt cgcggctgta gcaattgcgg tactcgcggt attgaatctg
      781 tgtggtgtac gccgcacggg cgtctatatt cttgttggcg tggtgttgtg gactgcggtg
      841 ttgaaatcgg gggttcacgc aactctggcg ggggtaattg tcggcttctt tattcctttg
      901 aaagagaagc atgggcgttc tccagcgaag cgactggagc atgtgttgca cccgtgggtg
      961 gcgtatctga ttttgccgct gtttgcattt gctaatgctg gcgtttcact gcaaggcgtc
     1021 acgctggatg gcttgacctc cattctgcca ttggggatca tcgctggctt gctgattggc
     1081 aaaccgctgg ggattagtct gttctgctgg ttggcgctgc gtttgaaact ggcgcatctg
     1141 cctgagggaa cgacttatca gcaaattatg gtggtgggga tcctgtgcgg tatcggtttt
     1201 actatgtcta tctttattgc cagcctggcc tttggtagcg tagatccaga actgattaac
     1261 tgggcgaaac tcggtatcct ggtcggttct atctcttcgg cggtaattgg atacagctgg
     1321 ttacgcgttc gtttgcgtcc atcagtttga caggacggtt taccggggag ccataaacgg
     1381 ctcccttttc attgttatca gggagagaaa tgagcatgtc tcatatcaat tacaaccact
     1441 tgtattactt ctggcatgtc tataaagaag gttccgtggt tggcgcagcg gaggcgcttt
     1501 atttaactcc acaaaccatt accggacaga ttcgagcgct ggaagagcgc ctgcaaggca
     1561 aattatttaa acgcaaggga cgtggtctcg aacccagcga gctgggagaa ctggtctatc
     1621 gctatgccga taaaatgttc accttaagcc aggaaatgct ggatattgtg aactatcgca
     1681 aagaatccaa tttattgttt gacgttggcg tggctgatgc actttccaaa cgcctggtca
     1741 gtagcgtact taacgccgca gtggtagaag gcgagcccat tcatcttcgc tgcttcgaat
     1801 ccacccacga aatgctgctg gagcaattaa gtcagcataa actggatatg atcatttctg
     1861 actgtccgat agactctacg cagcaggaag gcctgttctc cgtgagaatt ggcgaatgtg
     1921 gcgtgagttt ctggtgtaca aatccaccac cagaaaaacc gttcccggct tgtctggaag
     1981 aacggcgact tttgattcct gggcgacgtt caatgttagg gcgcaaattg cttaactggt
     2041 ttaactccca gggattaaac gtagaaatcc tcggcgagtt tgatgatgcc gctttgatga
     2101 aagcttttgg tgcgatgcac aatgcaatct tcgttgcccc aacgctttat gcatatgact
     2161 tttatgccga taaaactgtc gtagaaattg gtcgcgtcga gaatgtgatg gaagagtacc
     2221 atgctatttt tgctgagcgg atgattcagc acccggcggt acagcgaatc tgcaatacgg
     2281 attattctgc gctttttagt ccagcggtgc gttaatcggc agctccccca aagttaaggt
     2341 gggggagata gattagttgt acattaccac gattttgact cggctcatta tttgcccgct
     2401 tgagacattg tttccatatg tacgcgggcg aataaataga ggaatctgat tacttccttc
     2461 atggggatgc tgaaaagagt agtaattgct tttaccatac tggatttcat tcgtaccatc
     2521 attggagagt tgaaagccta tttgacccgc accgtcctca ctgttttcta actcattttt
     2581 taatatgctg gttcctgact cctgtatcgt cactggttca aagtaatatt caacgtcact
     2641 gggttcaaaa actaattcac cttgctcgcc ctggatgcag tcaaatctta tatgatgaaa
     2701 ctgataat
//
LOCUS       DAZPFP010000297         2694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPFP010000297 DAZPFP010000000
VERSION     DAZPFP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.302"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(4..318)
                     /locus_tag="ACHUR4_004004"
     CDS             complement(4..318)
                     /locus_tag="ACHUR4_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457352.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(360..866)
                     /locus_tag="ACHUR4_004005"
     CDS             complement(360..866)
                     /locus_tag="ACHUR4_004005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457353.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(1012..1611)
                     /locus_tag="ACHUR4_004006"
     CDS             complement(1012..1611)
                     /locus_tag="ACHUR4_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457354.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(1782..2693)
                     /locus_tag="ACHUR4_004007"
                     /pseudo
     CDS             complement(1782..2693)
                     /locus_tag="ACHUR4_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          992 a          530 c          383 g          789 t
ORIGIN      
        1 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
       61 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
      121 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
      181 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
      241 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
      301 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
      361 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
      421 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
      481 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
      541 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
      601 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
      661 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
      721 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
      781 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
      841 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
      901 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
      961 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
     1021 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
     1081 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
     1141 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
     1201 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
     1261 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
     1321 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
     1381 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
     1441 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
     1501 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
     1561 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
     1621 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
     1681 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
     1741 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
     1801 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
     1861 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
     1921 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
     1981 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
     2041 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
     2101 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
     2161 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
     2221 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
     2281 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
     2341 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
     2401 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
     2461 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
     2521 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
     2581 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
     2641 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aatt
//
LOCUS       DAZPFP010000298         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPFP010000298 DAZPFP010000000
VERSION     DAZPFP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.303"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHUR4_004008"
     CDS             <1..893
                     /locus_tag="ACHUR4_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1457355.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHUR4_004009"
     CDS             894..2624
                     /locus_tag="ACHUR4_004009"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1457356.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZPFP010000299         2654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPFP010000299 DAZPFP010000000
VERSION     DAZPFP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.304"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACHUR4_004010"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACHUR4_004010"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1457357.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACHUR4_004011"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACHUR4_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1457358.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHUR4_004012"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHUR4_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1457359.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2479..>2654
                     /locus_tag="ACHUR4_004013"
     CDS             2479..>2654
                     /locus_tag="ACHUR4_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1457360.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          734 a          623 c          649 g          648 t
ORIGIN      
        1 attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 ctccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccactgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat
     1561 tattcacggt gatgcctttg ccgaacttaa aaagctacct actgaaagcg tcgatctgct
     1621 ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca ttatgaacag tacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt
     1861 acaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccatac aatcatcaaa aagtaccggg
     2041 taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaga accacccgac
     2101 gcaaaaaccg aaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccatcg ccagcggacg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactccg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa
     2341 cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa
     2401 gtgtaagcct agttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctgacaac agaaacagac
     2581 aaatgccgta atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaat
//
LOCUS       DAZPFP010000300         2607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPFP010000300 DAZPFP010000000
VERSION     DAZPFP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.305"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            60..557
                     /locus_tag="ACHUR4_004014"
                     /pseudo
     CDS             60..557
                     /locus_tag="ACHUR4_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            598..789
                     /locus_tag="ACHUR4_004015"
                     /pseudo
     CDS             598..789
                     /locus_tag="ACHUR4_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(883..1089)
                     /locus_tag="ACHUR4_004016"
                     /pseudo
     CDS             complement(883..1089)
                     /locus_tag="ACHUR4_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1142..1393)
                     /locus_tag="ACHUR4_004017"
     CDS             complement(1142..1393)
                     /locus_tag="ACHUR4_004017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457361.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            1845..2129
                     /locus_tag="ACHUR4_004018"
     CDS             1845..2129
                     /locus_tag="ACHUR4_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR1457362.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            2129..2404
                     /locus_tag="ACHUR4_004019"
     CDS             2129..2404
                     /locus_tag="ACHUR4_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1457363.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          704 a          650 c          661 g          592 t
ORIGIN      
        1 agcgccagcc ccgtcccgcc cgatagagcg cggcatagcg ggccccggat tacttgcgtg
       61 cgacggcaga agaagccctg aagcgaatca gtgaactgta cgccatcgag gatgaaatac
      121 ggggattacc agagtcagag tgtcttgcag tcaggcagca gcgaagcaaa gcgttactga
      181 cgtcgctgca tgaatggatg gtggagaaga atggcacgct gtcgaaaaaa tccagactgg
      241 gcgaagcatt cagctatgta ctgaatcagt gggacgccct ctgttattac agtgatgacg
      301 gtctggcgga ggcggacaat aacacagcgg aaagagcgct tcgtgcagtc tgtctcggaa
      361 agaaaaatta cgtgttcttc ggtagcgatc acggcggcga gcgtggtgca ctgctgtacg
      421 ggctgatcgg cacctgccgt ctgaacggta tcgatccgga agcgtatctg cgccatattc
      481 tgagcgtact gccggaatgg ccttccaacc gagttgacga cctcctgcca tggaaggtgg
      541 tgctaccatc aggataatcg tcaatacggt gttcggtgaa cgtttacttg gagcatcatg
      601 cttcatgaag aaaaactggc acgtcatcaa cgtaaacagg cgatgtatac ccgaatggca
      661 gccttcccgg cggtgaaaat gttcgaagag tatgacttca cgttcgccac cggagcaccg
      721 cagaagcagc tccagtcgtt acgctcactc agcttcatag agcgcaatga aaacatagtc
      781 ttactgggga catctgacat cacaaatccc cgggtgggga tttgtgtata agagacagcc
      841 catggaggaa ggagatatat cgcagctctg acgactcact ccttatttac ccgggaaata
      901 cttctccagc aatgcataca ggatggcagc ggtttcagca tccattatgc cgtcataatt
      961 tttctgccgg aaatgaagct gaaaagcgcg gacaagcgcc ttatatccgt catccgtctc
     1021 cacaccagaa atactgtatc cataggtctt cagccgtgac acgatatcaa ccttatccgg
     1081 aatgccctgc gattcaaaaa ataaatattt agtcagtaat tttatgtttt tcctgaaaaa
     1141 attactcttt ttcgaactcc agtgagcgtt cacgactcat cagtgtgaaa atgatggcaa
     1201 aaacagcaga cagtatcagt acgataaatg tggcccccca tcccagataa tcgacaacaa
     1261 gtcccattcc cagtgtcgca acaattgagc cgaagatata ggaaaatgtg cctgacagcc
     1321 ctaacagaat aagtttacgc gtaaatcgcc ctgaatccag aagagcatta agtatcacat
     1381 aaacaggttc cataccagtc ctgaccgcat cagaacgcca gtaaataaaa tctcgtagtc
     1441 caccggcatc agagacaaga gtaatcgtcg ttaccggtat ttcgactggt ttgtactggt
     1501 gtgagcggac aaaactactg tacgtctctc atacacgctc gtaagcggcc ctcacggccc
     1561 gctaacgcgg agatacgccc cgactgcggg taaacccttg tcgggaccac tccgaccgcg
     1621 cacagaggca ttttcatcgc tgacaggccg catggcctcg cagagccgga gggggactgg
     1681 tgatattcgg acgaccgtac cggcttgcgc cgggcttcgg cggttttaca cctgtgctac
     1741 atgtaaccac cgtatgccgc cttatatgcg ctgactcgca ttatttgcct tgttccgtct
     1801 gaaagtaatc actatgatta aatatgatta acaactaatc gggtatgcaa atgaaaaaca
     1861 ataccgcaca agcaacaaaa gtaattaccg cgcatgtgcc attacctatg gctgataaag
     1921 tcgaccagat ggccgccaga ctggaacgct cccggggctg ggttatcaaa caggcgcttt
     1981 ctgcatggct tgcccaagag gaggagcgta atcgcctgac gctggaagcc ctggacgatg
     2041 tgacatccgg acaggttatc gaccatcagg ctgtacagtc ctgggcggac agcctcagta
     2101 ctgaccatct gttaccggtg ccacgctgat ggaactgaag tggaccagta aggcactttc
     2161 tgatttggcg cggttatatg attttctggt gctggccagt aaacctgctg ccgccagaac
     2221 ggtgcagtcc ctgacacagg cgccggtcat tctgttaact catccacgta tgggagaaca
     2281 gttgtttcag tttgaaccca gggaggtcag acggattttt actggcgagt acgaaatccg
     2341 ttacgaactt actggccaga ctatttatgt attgcgcctg tggcacacac gagagaacag
     2401 gtagattggg tgtggaggca actttaacat agcctgcagg tattaattct ggagcattgt
     2461 tatcaggcca gatattttgc attccattaa ataatcttgt aggagataaa ataaattacg
     2521 atgaaataac gaaaattacc gctcgtgact ggcggcaata tcgcgctccg ggctggcaaa
     2581 taacccatca gaaacgttat tgccaga
//
LOCUS       DAZPFP010000301         2580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPFP010000301 DAZPFP010000000
VERSION     DAZPFP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.306"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            79..513
                     /gene="slyA"
                     /locus_tag="ACHUR4_004020"
     CDS             79..513
                     /gene="slyA"
                     /locus_tag="ACHUR4_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1457364.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(560..1027)
                     /gene="slyB"
                     /locus_tag="ACHUR4_004021"
     CDS             complement(560..1027)
                     /gene="slyB"
                     /locus_tag="ACHUR4_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1457365.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1301..2410
                     /gene="anmK"
                     /locus_tag="ACHUR4_004022"
     CDS             1301..2410
                     /gene="anmK"
                     /locus_tag="ACHUR4_004022"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1457366.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            2508..>2580
                     /locus_tag="ACHUR4_004023"
     CDS             2508..>2580
                     /locus_tag="ACHUR4_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR1457367.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
BASE COUNT          673 a          637 c          636 g          634 t
ORIGIN      
        1 tgttttagca atacaattgc tgcacactat tctaaaagcc gcataatatc ttagcaagct
       61 aattataagg agatgaaatt ggaatcgcca ctaggttctg atctggcacg gttggtgcgc
      121 atatggcgtg ctctgataga ccatcgcctg aaaccgctgg agttaacaca aacccattgg
      181 gttacgttac acaatatcca tcagttacct ccagaccagt cgcaaattca actggcaaaa
      241 gcgattggca tcgagcagcc atcactggtc cgtactctgg accaactgga agaaaaaggg
      301 ttaatttcgc gtcaaacttg tgccagcgat cgtcgggcta aacgtattaa actgacggaa
      361 aaggcagagc cactgatcag cgaaatggaa gctgttatta acaaaacccg cgcggaaata
      421 ttacatggca tctccgcaga ggaactggag caactgatta cgctcatcgc aaaacttgag
      481 cataatatca ttgagttaca ggccaaaggg tgaaatgaag ggggcgtgtg gtcagtaacc
      541 tgaccacacg caaacttatt tagcgcggag aaacggtcac ctgactgcca ttgctggcca
      601 gtacgacacg ttggcccgga gagaaacgag tgttgccttg tttctgtacc accatgatgg
      661 tattaccatc gtctttacga atttccagct cgacaccctg cgttttgttc attgcactct
      721 gtacgccctg accagctacg ccacctgcaa cagcgcctgc tgcagtagcc agagaacgcc
      781 cggttccgcc accaacagta ttccccagga aaccaccaag aacagcaccg ccaattgcac
      841 cgataacgtt ggaatcatca ccgccctgaa tctgtaccgg acgtacgtta acgatggtgc
      901 catagctgac attctgtact tgtttcgctt cagaagcggt ataaacatcc cctgacaggg
      961 tgtcgttatt aacacaaccg acaagagaca gacctaccat tgaaacaacc aatacgcgtt
     1021 taatcattga aaaatctcct gttcaccatg aaacgccacg caagcatccc tcatggtcaa
     1081 agtatatggc atatgtgaat ccatggttac atgttctacc aaatatgagc aaatcataat
     1141 gaaagtagga tcaaccaatt ataaacaaaa cattcatgat gattattgat ttgaagataa
     1201 tgttttacta ccaacggtaa aaatcggtca ttccgcatgg tattcacgaa aagtttatgt
     1261 ttgagtggtg cgcaaacgtc agattgaaaa aggatgaact atgaaatcgg gccgctttat
     1321 tggcgttatg tcaggcacca gccttgatgg tgttgatgtt gtgttggcga caattgatga
     1381 acaccgggtc gcacagctgg caagcttgag ctggccgatc ccggtgtctc tgaaacaggc
     1441 tgtactggat atttgccagg gccagcagct tacactttcg cagtttggac agcttgatac
     1501 tcaactcggg cgactttttg ctgatgcggt caatgccttg cttaaggaac aaaacctgca
     1561 ggcgagagat atagttgcga tcggctgtca cggtcaaacc gtctggcatg aaccgacggg
     1621 cgtggcacca cacactttac agattggcga taacaatcaa attgtggcac gcaccggaat
     1681 tacggttgtc ggtgattttc gccgtcgcga tattgccttg ggaggacaag gcgcaccgct
     1741 ggtacctgcg ttccatcatg ccttgctggc tcacccaacc gagcgacgaa tggtgctcaa
     1801 tattggcggc atcgccaatc tgtcactgct cattcctggg cagctggttg gcggctacga
     1861 taccggtcct ggtaacatgc tgatggatgc ctggatctgg cgtcaggcag gtaaacctta
     1921 cgataaagat gccgagtggg cacgggcggg taaagttatt ctcccactgc tgcaaaatat
     1981 gctcagcgac ccgtatttct cgcaacctgc accgaaaagc accggacgcg aatactttaa
     2041 ctacggctgg ctggagcgcc atttacgcca ttttccgggt gttgatcccc gtgatgttca
     2101 ggccacactg gcagaactca ccgccgtgac catttctgaa caagttttgt tgagcggtgg
     2161 ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat ccgctactca tggcgcgtct
     2221 ggcggtatta ctgctaggca cagaagtcac caccaccgat gccgttggca ttagtggcga
     2281 tgacatggaa gcattggctt tcgcctggct tgcctggcgg acgctggcgg gattaccagg
     2341 aaatctgcct tccgtcactg gcgcaagcca ggagacggta ctgggggcta ttttccccgc
     2401 caactcgtga tatcagaatt aactgaattt tttcttatcg tgtctgccgt tacacttgga
     2461 agattaagga gggtatttgc cctccggacc aggaaagtct tcgggatatg actatgaaaa
     2521 aactgttaat tatcattttg ccggtccttc tctccggttg tagcgccttt aatcaactgg
//
LOCUS       DAZPFP010000302         2567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPFP010000302 DAZPFP010000000
VERSION     DAZPFP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.307"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            113..448
                     /gene="eutS"
                     /locus_tag="ACHUR4_004024"
     CDS             113..448
                     /gene="eutS"
                     /locus_tag="ACHUR4_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1457368.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            461..940
                     /gene="eutP"
                     /locus_tag="ACHUR4_004025"
     CDS             461..940
                     /gene="eutP"
                     /locus_tag="ACHUR4_004025"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1457369.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            915..2288
                     /gene="eutA"
                     /locus_tag="ACHUR4_004026"
     CDS             915..2288
                     /gene="eutA"
                     /locus_tag="ACHUR4_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1457370.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(2359..>2567)
                     /locus_tag="ACHUR4_004027"
     CDS             complement(2359..>2567)
                     /locus_tag="ACHUR4_004027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457371.1"
                     /translation="RTTRRTCEDLSILSFLVFIFHFLFSSLSGSVLVLVFSFLFLFFL
                     QGYAKTPKHFSPQKHPIILPDQSP"
BASE COUNT          612 a          644 c          724 g          587 t
ORIGIN      
        1 ctctccattc ttattgatct acctcacctt ttaaacgcgc ttgccgaatt ttgttattta
       61 ctctgacgaa aaattgtcac gatacacgaa agtttttcac aggcggcgac tcatggataa
      121 agaacgcatc attcaggaat ttgtgcccgg caaacaggtc acactggcgc atctcatcgc
      181 gcatcccggt gaggaactgg cgaagaaaat cggcgttccg gatgcgggcg caattggcat
      241 tatgacgcta actcccggcg aaacggcgat gatcgccggt gatttagcgc tgaaagccgc
      301 tgatgtgcat atcggttttc tcgaccgctt cagcggtgca ttggtgattt atggctcggt
      361 gggcgcagta gaagaagcgt tatcacaaac cgtcagcggt ttaggtcgct tattaaatta
      421 tacgctttgt gaaatgacca aaagttaata cgaggcattc atgaaacgaa ttgcgtttgt
      481 gggtagcgtc ggggcaggaa aaacaacgtt atttaatgcg ttgcagggga attataccct
      541 cgccagaaaa acactggccg tggaatttaa tgataagggc gatattgata cgccgggtga
      601 atattttagc catccccgct ggtatcacgc cttaattacc acgctacagg atgtagacat
      661 gctgatctat gtccatggcg caaatgaccc ggaaagtcgg ttgcctgccg ggttgctgga
      721 tattggcgtc agcaaacggc aaatcgccgt catcagtaaa acggacatgc cagatgctga
      781 cgtcgccgca acgcgaaagt tattgcttga gacgggtttc gaagagccga ttttcgagct
      841 aaacagccac gacccgcaaa gcgtgcagca actggtggat tatctggcat ctctcaccaa
      901 acaggaggaa gcaggtgaaa aaactcatca cagcgaatga tattcgtgaa gcacacgcgc
      961 gcggcgaaca ggcaatgtcc gtggttctgc gcgccagcat tattaccccg gaagcccgtg
     1021 aagtggcgga tctactgggt tttaccatca ccgaatgtga tgaatcgatc ccggtaacgg
     1081 cgtccgtgcc cgccagcgtg ccagccgaca aaaccgaaag ccagcgcatc cgcgaaacca
     1141 tcattgccca gctgccggaa ggccagttta ccgaaagcct ggtcgcacag ctgatggaaa
     1201 aagtgatgaa ggaaaaacag tcgctgggcg attttgtcgt cgccagcgcc gggccgcacc
     1261 tcgaatccgt gatcgccggt cacggagccg gggcgcaaac cctttctgaa caacggctgt
     1321 gtcgggtact gaatatcgat atcggcggtg gcaccgcgaa ctacgccctg ttcgatgccg
     1381 gaaaaatcag cggcactgcc tgtctcaacg tcggtggtcg cctgctggaa accgacagcc
     1441 aggggcgcgt ggtttacgct cataaaccgg ggcagatgat tgtggatgag tgcttcggtg
     1501 caggcactga cgcccgttcg ctgaccggcg cgcagctggt gcaggttacc cggcggatgg
     1561 cggcgctgat tgtcgaagtg attgacggaa cgctttcgcc gctcgcgcag gcattgatgc
     1621 aaaccggttt gctgcccgca ggtgttacgc ccgaaatcat tacgctttct ggaggcgtgg
     1681 gcgaatgtta tcgccaccag cccgccaacc cgttctgttt tgccgatatt ggcccacttc
     1741 tggcaacggc gctgcatgac catccgcgcc tgcgtgagat gaatgtgcag tttccggcgc
     1801 aaaccgtacg ctccacggtg attggcgcgg gtgcacatac cctttcgctc tctggcagca
     1861 caatctggct ggagggcgta caactgccgc tgcgcaattt gccggtggcg atcccgattg
     1921 atgaaacgga tctggtgagt gcctggcaac aggcgctgct tcagctggat cttgatccca
     1981 aaactgacgt gtacgtgctg gcgcttcccg cctcgctgcc tgtgcgttac gccgcggttc
     2041 tgacggtcat caacgcgctg gtcgatttcg tcgcgcgttt tccgaatccg catcccctgc
     2101 tggtggtggc cgggcaggac tttggtaaag ctctgggcat gttgttgcgc ccacagctac
     2161 aacaactccc gttggcagtc attgacgaag tgattgtccg cgcgggggac tatatcgaca
     2221 ttggtacgcc tctttttggc ggatcggttg tgccggtgac ggtgaaatca ctcgcatttc
     2281 cttcctgaat acggtagtta tcaggtatgg tgcttggtgt ctgggtgtta gtgggattat
     2341 atccggcagt gtatccggtt aaggtgattg gtctggcaag atgattggat gcttttgtgg
     2401 tgaaaaatgt tttggtgttt tagcataacc ctgaagaaag aataaaaata aaaaggaaaa
     2461 aaccaaaacc agaacagaac cggaaagaga agaaaagaga aaatgaaaaa taaaaaccaa
     2521 aaaagaaaga atacttaaat cttcgcatgt tcttcgggtg gtacgca
//
LOCUS       DAZPFP010000303         2557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPFP010000303 DAZPFP010000000
VERSION     DAZPFP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.308"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUR4_004028"
     CDS             <1..197
                     /locus_tag="ACHUR4_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1457372.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHUR4_004029"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHUR4_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR1457373.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUR4_004030"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUR4_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR1457374.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..>2557
                     /locus_tag="ACHUR4_004031"
     CDS             1821..>2557
                     /locus_tag="ACHUR4_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR1457375.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNF"
BASE COUNT          610 a          607 c          659 g          681 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttat
//
LOCUS       DAZPFP010000304         2547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPFP010000304 DAZPFP010000000
VERSION     DAZPFP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.309"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..448
                     /locus_tag="ACHUR4_004032"
     CDS             <1..448
                     /locus_tag="ACHUR4_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457376.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            798..1160
                     /locus_tag="ACHUR4_004033"
     CDS             798..1160
                     /locus_tag="ACHUR4_004033"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR1457377.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1157..2074
                     /locus_tag="ACHUR4_004034"
     CDS             1157..2074
                     /locus_tag="ACHUR4_004034"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1457378.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            2087..>2547
                     /locus_tag="ACHUR4_004035"
     CDS             2087..>2547
                     /locus_tag="ACHUR4_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR1457379.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISE"
BASE COUNT          756 a          492 c          549 g          750 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag
      481 tcgaacctac gaccttcgca ttacgaatct gtagcaccaa tcataactat ctgttttagc
      541 aagcattaac cgcattcact aagatatagt tgatggcaca aacagaaagt tgatgaatga
      601 tgttgccata tatatgtcac aaatacggca caacgatctt caaacatata gccacacatt
      661 cacagaagag cacaaagcct tgcaatccag tgcaaagatt tgtgtgccag agtttttctc
      721 atcaactacc gcaagtatcg atcgattgag acttggatga tagacttcat gcctttgatt
      781 attagctgat agaagaaatg ttaaagctat ttgcaaagta cacatctata ggcgtgctga
      841 acacacttat acactgggtg gtttttggtg tatgtatcta tgccgcgcat accaatcagg
      901 ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt tagcttcttt gcgaatgcaa
      961 aattcacatt caaagcatca actacaacga tgcgctacat gttatatgtc gggttcatgg
     1021 gaacactgag tgctactgtt ggatgggttg ctgatagatg ttcacttcct ccaattgtca
     1081 ctcttgttac cttctccgcc atcagcttgg tgtgcggttt cgtctattca aagttcattg
     1141 tctttaggga tgcgaaatga agatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1201 gataccaatt ttttataaaa cggtacgtga attcgaagag ttgaagccat atgaagtaga
     1261 aattgttttc ataaatgacg gaagcaaaga cgctacggag tcaatcatta acgctctggc
     1321 tgtttctgat ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1381 attgtttgca gggttagacc atacaaccgg cgatgccgtg attccaattg atgttgacct
     1441 gcaagacccg attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgacat
     1501 ggtgcttgct aaacgttcag accgctcaac tgatggacgg ctgaaacgta agacagctga
     1561 gtggttctat aaattacaca acaaaataag caccccaaaa atagaggaaa atgtcggaga
     1621 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctattgcctg agcgcaatct
     1681 tttcatgaaa ggcatactga gctgggttgg tggtcagaca gatgtcgttg aatatgtacg
     1741 cacagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1801 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1861 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacacca ttgtctttgg
     1921 taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg gtggagtgca
     1981 actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag aaacaaaatc
     2041 aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga agatattcat
     2101 aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga tttggcttag
     2161 acgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt tttggtataa
     2221 agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg gttattttca
     2281 aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt acgcaccatt
     2341 attatcaaac ttttttggaa taataattag agctataatt atatggttct tatttaccga
     2401 tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt taatttgcat
     2461 gcccgggctt gatgaagtcc aagccaatat aacaaatgct cactggtatt tatcattgta
     2521 tgttgcaatg ataataatat ctgaaga
//
LOCUS       DAZPFP010000305         2524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPFP010000305 DAZPFP010000000
VERSION     DAZPFP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.310"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUR4_004036"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUR4_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUR4_004037"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUR4_004037"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1457380.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUR4_004038"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUR4_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT          719 a          638 c          538 g          629 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaa
//
LOCUS       DAZPFP010000306         2491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPFP010000306 DAZPFP010000000
VERSION     DAZPFP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.312"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>2491
                     /locus_tag="ACHUR4_004039"
     CDS             <1..>2491
                     /locus_tag="ACHUR4_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1457381.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZPFP010000307         2483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPFP010000307 DAZPFP010000000
VERSION     DAZPFP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.313"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..823)
                     /locus_tag="ACHUR4_004040"
     CDS             complement(<1..823)
                     /locus_tag="ACHUR4_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457382.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="ACHUR4_004041"
     CDS             complement(1422..1670)
                     /locus_tag="ACHUR4_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1457383.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1933..2028)
                     /locus_tag="ACHUR4_004042"
                     /pseudo
     CDS             complement(1933..2028)
                     /locus_tag="ACHUR4_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2025..2372)
                     /locus_tag="ACHUR4_004043"
                     /pseudo
     CDS             complement(2025..2372)
                     /locus_tag="ACHUR4_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            complement(2399..>2483)
                     /locus_tag="ACHUR4_004044"
     CDS             complement(2399..>2483)
                     /locus_tag="ACHUR4_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457384.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          638 a          468 c          605 g          772 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg gagtgcccct attaagttgt caagcatgtt
     2401 atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc
     2461 gtcgtctcgc cagggcgata agc
//
LOCUS       DAZPFP010000308         2450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPFP010000308 DAZPFP010000000
VERSION     DAZPFP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.314"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..565
                     /locus_tag="ACHUR4_004045"
     CDS             <1..565
                     /locus_tag="ACHUR4_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457385.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACHUR4_004046"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACHUR4_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1457386.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHUR4_004047"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHUR4_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1457387.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="ACHUR4_004048"
     CDS             2167..2403
                     /locus_tag="ACHUR4_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1457388.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          630 a          535 c          632 g          653 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag
//
LOCUS       DAZPFP010000309         2441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPFP010000309 DAZPFP010000000
VERSION     DAZPFP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.315"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHUR4_004049"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHUR4_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1457389.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUR4_004050"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUR4_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1457390.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUR4_004051"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUR4_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1457391.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2441
                     /locus_tag="ACHUR4_004052"
     CDS             1993..>2441
                     /locus_tag="ACHUR4_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457392.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINA"
BASE COUNT          637 a          564 c          597 g          643 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt g
//
LOCUS       DAZPFP010000310         2436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPFP010000310 DAZPFP010000000
VERSION     DAZPFP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.316"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHUR4_004053"
     CDS             <1..360
                     /locus_tag="ACHUR4_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1457393.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHUR4_004054"
     CDS             361..726
                     /locus_tag="ACHUR4_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1457394.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHUR4_004055"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHUR4_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHUR4_004056"
     tRNA            1605..1680
                     /locus_tag="ACHUR4_004056"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHUR4_004057"
     tRNA            1682..1758
                     /locus_tag="ACHUR4_004057"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHUR4_004058"
     tRNA            1761..1836
                     /locus_tag="ACHUR4_004058"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHUR4_004059"
     tRNA            1840..1914
                     /locus_tag="ACHUR4_004059"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHUR4_004060"
     CDS             2207..2422
                     /locus_tag="ACHUR4_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1457395.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAZPFP010000311         2408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPFP010000311 DAZPFP010000000
VERSION     DAZPFP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.317"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..108
                     /locus_tag="ACHUR4_004061"
                     /pseudo
     CDS             1..108
                     /locus_tag="ACHUR4_004061"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            60..233
                     /locus_tag="ACHUR4_004062"
     CDS             60..233
                     /locus_tag="ACHUR4_004062"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR1457396.1"
                     /translation="MPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALR
                     MLQEYIRIQCLGG"
     gene            complement(322..834)
                     /locus_tag="ACHUR4_004063"
     CDS             complement(322..834)
                     /locus_tag="ACHUR4_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1457397.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1029..1793
                     /locus_tag="ACHUR4_004064"
     CDS             1029..1793
                     /locus_tag="ACHUR4_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457398.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1744..>2408
                     /locus_tag="ACHUR4_004065"
     CDS             1744..>2408
                     /locus_tag="ACHUR4_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1457399.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          677 a          538 c          611 g          582 t
ORIGIN      
        1 atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga aaatgccctg
       61 tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc accgtcgaac
      121 tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc agcgaccaga
      181 cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg tagcggtaat
      241 tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa gctggattcg
      301 caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa aactaccaac
      361 ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt tcactggatg
      421 aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga taaagcgcga
      481 agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt ctattgtatt
      541 ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta taccaagatg
      601 gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag cttcccatgg
      661 tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg ctggtggcaa
      721 tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa gcccccatag
      781 gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca tcaatgtctt
      841 tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta ggcttaaaca
      901 ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc aggagtgagt
      961 gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa atcacaggaa
     1021 gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg gccggagtcc
     1081 ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc aggaagaggg
     1141 ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa
     1201 aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa aaaagtacgc
     1261 aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt accgatcccc
     1321 cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt tatgcccgtc
     1381 gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc gaggacgaat
     1441 tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt
     1501 cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat atcagcgccg
     1561 ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc acgcttgcga
     1621 cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat aaggtgtctc
     1681 tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga gagcgtgacc
     1741 tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg tagtttctgg
     1801 actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa atcatgggat
     1861 gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg ttgtgcccgt
     1921 cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca gatagacagg
     1981 tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg ggttactggc
     2041 tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc actggagggg
     2101 atcgatatgt tgtggttgga agaagcccac gcactgacgg aataccagtg gaaaattctg
     2161 gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc cggacttgtt
     2221 actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct catccgcaaa
     2281 attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat cgacgcggcg
     2341 cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga gtctgatgat
     2401 gatgcggc
//
LOCUS       DAZPFP010000312         2333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPFP010000312 DAZPFP010000000
VERSION     DAZPFP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.318"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            19..528
                     /gene="eco"
                     /locus_tag="ACHUR4_004066"
     CDS             19..528
                     /gene="eco"
                     /locus_tag="ACHUR4_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1457400.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(650..2274)
                     /gene="mqo"
                     /locus_tag="ACHUR4_004067"
                     /pseudo
     CDS             complement(650..2274)
                     /gene="mqo"
                     /locus_tag="ACHUR4_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT          546 a          642 c          583 g          562 t
ORIGIN      
        1 atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat tctacctgaa
       61 gtattgtttg ccgctttcgc taccacttcc gcctgggcgg cagaaagcgt ccagccactg
      121 gaaaaaatcg cgccttatcc acaagctgaa acaggcatga agcgtcaggt gattcagtta
      181 accccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa
      241 gtcgattgca atttgcatcg tctcggcggg aagctggaaa gcaaaacgct ggaaggctgg
      301 ggctatgatt attatgtctt tgataaagtt agctcgccgg tttccacgat gatggcctgc
      361 ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg
      421 cgttacaaca gcaagctgcc gatcgtggtg tatacgccag acaatgtaga tgtgaaatac
      481 cgcgtctgga aggcggaaga gaaaattgac aacgcggaag ttcgctaaac tgttgtgaag
      541 tacggcaccc cgttggttgg ataaggcggt tacgccgcat ccaacatcta acgcccgagc
      601 cggttgcctg atgcgacgct ggcgcgtctt atcagtatac gccacatcat tacaacgcaa
      661 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
      721 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
      781 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
      841 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagt agattcaaca
      901 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
      961 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
     1021 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
     1081 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
     1141 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
     1201 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
     1261 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
     1321 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
     1381 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
     1441 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
     1501 taaagacaaa tttggcgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
     1561 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
     1621 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
     1681 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
     1741 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
     1801 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
     1861 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
     1921 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
     1981 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
     2041 gttgttctag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
     2101 cgaccattca ggctccagct cgcgtcaata ggtccccaac gtggcgctca taatgccgcc
     2161 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
     2221 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
     2281 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acg
//
LOCUS       DAZPFP010000313         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPFP010000313 DAZPFP010000000
VERSION     DAZPFP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.319"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHUR4_004068"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHUR4_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1457401.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHUR4_004069"
     CDS             282..452
                     /locus_tag="ACHUR4_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR1457402.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHUR4_004070"
     CDS             527..802
                     /locus_tag="ACHUR4_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457403.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHUR4_004071"
     CDS             902..>2325
                     /locus_tag="ACHUR4_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1457404.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZPFP010000314         2300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPFP010000314 DAZPFP010000000
VERSION     DAZPFP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.320"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..700)
                     /gene="ipaJ"
                     /locus_tag="ACHUR4_004072"
     CDS             complement(<1..700)
                     /gene="ipaJ"
                     /locus_tag="ACHUR4_004072"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR1457405.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMEL"
     gene            complement(897..1706)
                     /locus_tag="ACHUR4_004073"
                     /pseudo
     CDS             complement(897..1706)
                     /locus_tag="ACHUR4_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2053..>2300
                     /locus_tag="ACHUR4_004074"
     CDS             2053..>2300
                     /locus_tag="ACHUR4_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457406.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          660 a          588 c          397 g          655 t
ORIGIN      
        1 ctaattccat agttgaaaaa caagaataat tttctcctac agctggatcc atatagcttc
       61 catcaggtct acacaacacc cagtggaggc cgacaggaac tccaataaag gatacagcaa
      121 cagcttccaa aactctttgg ttagaagata acacatctcg ttgatgtact atattacatc
      181 ccatgccaat taataagtcc cttgcatcgg gataaataaa actaattact tgcgaaaaaa
      241 tgttactttc ttcaacgaca taagcattca atccaagaag cctcgtagcc atgactatct
      301 tatcaggcat agaataaccg gcatccgcga tattatcttt gtgtattctc ggattataat
      361 taccactagt tattaagtat aaatcccgct cacaacgatt atccaaatca agagaactct
      421 tccttgtcat ttcggacata gaccctttgt attgtggtat tttatcaacc cctaattcct
      481 ttgcagcaca gagtaaactg gcagctccac aagaattgtc atacatctgc ttcctcgcca
      541 aaaaacaacc ttgttcgttt actctattct catttaccct cacatcagtc tgatgagata
      601 tcatatattc tcttggtata gccccctgca ataaaacccc atataacata acacctgtat
      661 gaatacatcc ccgtttgcaa ggcttccgtt gttccgacat cgacgagtaa ttgcagcaca
      721 gtattgcatc cagcaatgcg aactactatg agaattagct gaccgggtcc ttattccccg
      781 aacgaacttt aaatccaaaa cctcatactc ttttccgtcg tcatcacgtc cgtcaccata
      841 acaacctttc agcatccacc cttttattaa gagttaatgt ccatcagcat cagcaattaa
      901 taccgcctga tactgacata tttttgtcgt atttacgtcc ataatcggtt attgtgatac
      961 aggtcacacc agcttatttg ctattgttac aataaccacc ttggttgaga gctgtttttt
     1021 caactttgtt atcaactccc ccaactcctt ggtcagtaag gctgccgacc gagcacagtg
     1081 aatagatagt atccgcaggt accaccatgc ttacttaata ctcagaaggc gaagtgctct
     1141 ccaattaact ctgccgcact atgcttataa gctgcttttc aatgccgtct atcaacatgt
     1201 ccaattcatt caatctgttg tactggtaat ccaacgcagt aatgatatat gcaggtattc
     1261 tttcatccac tccagtaacg tttcatgcaa agcattaatt tacgcgctac ggaacttcac
     1321 cagttgactg agcaaacgat gtgggaccag tactgcctgc agttcttcta ttgttacagc
     1381 gatttcttta ccaaacagct gaaccaccat caagataaag atagcctgaa cattcatcgc
     1441 accattttgt taccaatggc aaaagattta acgaaccgtg cctgcatcag gcggactttt
     1501 tatccctgct ttgttagctc cctaaccttg tacagagagc ctccataagc tcccatatca
     1561 agcagacatg gttcgcagtt actgacatac accagaaaac tctgacagca gactcactta
     1621 tcctccactt cactcatatc cttattggtg agatgaacct gaatgacatg ttttgcgaca
     1681 taaacgccaa ccagtgtata tttcacctgt agagattcta caatctgtag cgttatgcat
     1741 ctaatgttag gctctatgat gtcagaaatc tgcgagactc tacaccttct gcatttacct
     1801 ttatttatca ccatgctcct cagttaacgg agaacgtgtt cattgcattt tgtcacttac
     1861 atttcgcctt tggtatcggg gggatatagg ttccaacgtt ccgcatgaga acctgaaccg
     1921 gcttcacgcc accttgatac cagatgccgc acaggtagtc aacaagtgtc acgaacggtg
     1981 caatagtgat ccacacccaa cgcctgaaat cagatccagg gggtaatctg ctctcctgat
     2041 tcaggagagc ttatggtcac ttttgagaca gttatggaaa ttaaaatcct gcacaagcag
     2101 ggaatgagta gccggacgat tgccagagaa ctggggctct cccgcaatac cgttaaacgt
     2161 tatttgcagg caaaatctga gccgccaaaa tatacgccgc gacctgctgt tgcttcactc
     2221 ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc ttacaaaatc
     2281 ccggcaacgg taatcgctcg
//
LOCUS       DAZPFP010000315         2282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPFP010000315 DAZPFP010000000
VERSION     DAZPFP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.321"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(141..236)
                     /locus_tag="ACHUR4_004075"
                     /pseudo
     CDS             complement(141..236)
                     /locus_tag="ACHUR4_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(272..577)
                     /gene="ccdB"
                     /locus_tag="ACHUR4_004076"
     CDS             complement(272..577)
                     /gene="ccdB"
                     /locus_tag="ACHUR4_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR1457407.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(579..797)
                     /gene="ccdA"
                     /locus_tag="ACHUR4_004077"
     CDS             complement(579..797)
                     /gene="ccdA"
                     /locus_tag="ACHUR4_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR1457408.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1333..2282)
                     /locus_tag="ACHUR4_004078"
                     /pseudo
     CDS             complement(1333..2282)
                     /locus_tag="ACHUR4_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          530 a          621 c          556 g          575 t
ORIGIN      
        1 aaaattggct gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg
       61 tattgttctt gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct
      121 cgtcagaacc gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg
      181 cgtacccggt ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc
      241 aacgccacga tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg
      301 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga
      361 tcggcacact ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca
      421 ccgggtaaag ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca
      481 ccatccgtcg tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac
      541 gactctctct tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg
      601 gcaaaagagc cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca
      661 gcctgccagc gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg
      721 cctgagatat tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca
      781 gtaatacgct gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata
      841 tattaatggt acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt
      901 ttaatacaat tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg
      961 taagtaaatt acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt
     1021 ccggaagtgt aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg
     1081 cccacagaga atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg
     1141 ccgcgaagta ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt
     1201 tcaggctgat tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt
     1261 cctgttgtca ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac
     1321 ggcaggtatc actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg
     1381 ctcctccggc cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag
     1441 accattacgt tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat
     1501 cagtgaaccg gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc
     1561 gcagatattg ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg
     1621 ccggttcaga atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc
     1681 ctgctcttca agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca
     1741 ctgccgtatt ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc
     1801 gatttttctc agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa
     1861 gccgcgccct gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag
     1921 agatttgtac acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca
     1981 ggtctcaggg tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc
     2041 gcttctttca ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg
     2101 ggtatcaagg agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc
     2161 atggcgtagc agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc
     2221 cattgtactg acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag
     2281 gg
//
LOCUS       DAZPFP010000316         2270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPFP010000316 DAZPFP010000000
VERSION     DAZPFP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.322"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHUR4_004079"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHUR4_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1457409.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHUR4_004080"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHUR4_004080"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1457410.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHUR4_004081"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHUR4_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1457411.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          504 a          595 c          538 g          633 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac atcggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc
//
LOCUS       DAZPFP010000317         2267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPFP010000317 DAZPFP010000000
VERSION     DAZPFP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.323"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(550..2154)
                     /locus_tag="ACHUR4_004082"
     CDS             complement(550..2154)
                     /locus_tag="ACHUR4_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457412.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          586 a          552 c          503 g          626 t
ORIGIN      
        1 taagcccctg tagggactag agaccataat taagatgatt gtgtcttatt gctattcact
       61 tatcgactga cccagagcac acaggaagca ttcactttaa gtagaacgct tggtgtctgt
      121 aattggtcat cccgcccctg aaaggatgcg ataagttacg gttagttgtc ctagaagaga
      181 aaactttttg tgtcccttct ccctatagtg agtcgtatta attatccttg acaagtagag
      241 aaaaccatga gtcaaaaaca tgaccacaca ggtctgtagt aagtgtcatc ctagaagaga
      301 aaactttttg tgtcccttct ccctatagtg agtcgtatta attacccctt gacaagcaga
      361 gaaaactatg agtcaaaaac atgaccgcac aggtctgtag taagtgtcat cctagaagag
      421 aaaacttttt gtgtcccttc tccctatagt gagtcgtatt aattatccct tgacaagtag
      481 agaaaactat gagtcaaaaa catgaccaca caggtctgta gtaagtgtca tcctagacta
      541 tggtctcctc taggtctcct tataaacatc agccatcaca ttgaccagcc tatcaacctc
      601 gacctcttta gcataacggt cgtacgtaat agaccctgaa ctatgcccca tgatcgcctg
      661 tgcatacgcc aactcgaccc catgttgttt taactgggcc gcaactgtat gtctaaacga
      721 gtgaaacgct aagccgctac ggtctacgag gactttaggc aacaacgtcc gattaaacca
      781 tttactcacc tgctcaccgt aaccgttctt catgagacgc agaccatcga acaccatagc
      841 gtcatcaccg tcagcgcctc tacgggtctc tacaagcgcc ataaagtcag ccaatataaa
      901 accataggcc ccatcgacca gaggcacaca gcggtcacta tgagcgttct tgatgctctt
      961 accgggtaga ctactatcgt cttcattgat atgaatataa acggttcccg cctcagtggt
     1021 cctgacgtct ttgacctgta gttgggctat ttcattgagt ctcgccccag tgatagcagc
     1081 caaggcggta acataataat gatacggctt accagaggtc ttctgagagt atgccttagc
     1141 tgcgaccagt agttgcccca cctgctccgt agagaaggcg ttacgggccc ctgaggcttt
     1201 acgctgaggg actttaagct ccagaccctc agtcatgttc tttttaatca agtcattgcg
     1261 cacagcccac ctaaagacag cagccatctt aataagatac ttgttgttga tggtaacgac
     1321 atccagacaa tctgtcttat cttcacggct caacaggtca gcaagtggga catccttgaa
     1381 gcgctgctta cggttcctag gtaactgctg gagcacatca cggaccctaa gcatgtcagc
     1441 cctgttagcg tctgcattga gacccagatg gtcgaatatt tcgattaacg cagcgtgtga
     1501 cgccttgttt tcccttaagg ttgcaggctt ccagttctga tagttctccg cttcgtattg
     1561 ttcaaagagg ctcgctatgg taatgctagg cgtaacttct ggttttactt ctggagcatt
     1621 aacagatagg gacacagagt ctgcttggtt atcttgccta tcaatatcat taaaaagatc
     1681 gacgtaatct atcagaggct gactgtctcc cgcttcgata cgcctcatac aggcttttag
     1741 caccttcagg gcctcattga tgtaacgatg ctggtcaatg cttagcggct cactagccag
     1801 agcatcggta aggctctcac caagttctcc gtattggtcc cggtagatat ccctcatgtc
     1861 aggctcaaag agatctgaac ggcccataga cagttcacac tcagcgatgt ccttaaggtg
     1921 ttccctcatt tcctcatagg tagcgttggg tctgtctgcg tgtattgcct tgagtgctgc
     1981 ctttatgtgc cttgagtaag ccatagcggt gcgtctatcc ttggtccgta atgatattga
     2041 ggtcattcta tcattgtttg attgtctgac cctaagacgg aagtagtaga cgccgttacg
     2101 ctggtagaga tagtagactg ctggaagttt gtgactactg gatagctgaa tcatatgtct
     2161 gcatgccctg agagggttac acagtgggtt acagtaagta actctgcaag gaggcttaaa
     2221 gccgtatccc ttaggattca gtaggttgga gtactttaag aaaggtc
//
LOCUS       DAZPFP010000318         2260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPFP010000318 DAZPFP010000000
VERSION     DAZPFP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.324"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUR4_004083"
     CDS             complement(<1..242)
                     /locus_tag="ACHUR4_004083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457413.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUR4_004084"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUR4_004084"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1457414.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          559 c          668 g          459 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc
//
LOCUS       DAZPFP010000319         2227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPFP010000319 DAZPFP010000000
VERSION     DAZPFP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.325"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="ACHUR4_004085"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="ACHUR4_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1457415.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="ACHUR4_004086"
     CDS             580..1029
                     /locus_tag="ACHUR4_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1457416.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHUR4_004087"
     CDS             complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHUR4_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1457417.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHUR4_004088"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHUR4_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1457418.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="ACHUR4_004089"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="ACHUR4_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1457419.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          549 a          502 c          537 g          639 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagaaac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctga
//
LOCUS       DAZPFP010000320         2219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPFP010000320 DAZPFP010000000
VERSION     DAZPFP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.326"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUR4_004090"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUR4_004090"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1457420.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUR4_004091"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUR4_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1457421.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="ACHUR4_004092"
     CDS             complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="ACHUR4_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1457422.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          589 a          611 c          556 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatggg
//
LOCUS       DAZPFP010000321         2210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPFP010000321 DAZPFP010000000
VERSION     DAZPFP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.327"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUR4_004093"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUR4_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1457423.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHUR4_004094"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHUR4_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1457424.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHUR4_004095"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHUR4_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHUR4_004096"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHUR4_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          535 a          589 c          617 g          469 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt
//
LOCUS       DAZPFP010000322         2201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPFP010000322 DAZPFP010000000
VERSION     DAZPFP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.328"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(14..964)
                     /gene="virK"
                     /locus_tag="ACHUR4_004097"
     CDS             complement(14..964)
                     /gene="virK"
                     /locus_tag="ACHUR4_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1457425.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            complement(969..2057)
                     /gene="capU"
                     /locus_tag="ACHUR4_004098"
     CDS             complement(969..2057)
                     /gene="capU"
                     /locus_tag="ACHUR4_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1457426.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            complement(2060..>2201)
                     /locus_tag="ACHUR4_004099"
     CDS             complement(2060..>2201)
                     /locus_tag="ACHUR4_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HFR1457427.1"
                     /translation="GTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPRLP
                     DD"
BASE COUNT          658 a          513 c          419 g          611 t
ORIGIN      
        1 aatacatcaa accttaattt aagtcctgat gttctgagtt tacaggatat gcatccaaag
       61 agcggatagc agtttcatgg atataatcca gcaaagcgta tcgttttcga tactcagagc
      121 gttttttact agctatattg cttagcggct ttcgaaccac ctgtgttggt aatttgtacc
      181 agtcaccaca ggttttccct gctacagact cccagaaatc actgtagaca gcaacaaagg
      241 ttttgcgttt ctgataccag tatctcaatt gccggaaaac atgactgtgc tcactgactg
      301 ccagacactc agagacatta caagctttca tcaacgcaca aaatgcttca aaaataatcc
      361 gttttgggaa aagcccataa caggcccggg ttgcgcaccg aataatatca ggtccggtat
      421 ttttgggggc tccctgtaat ccgccaataa aagcggtgtg tccattttga gtcagaatga
      481 ctgaaaatgt taaccgggca atcactattt tattgaaaaa caatgacaac atcaactctc
      541 cttcccggtc aaaaccactg ggagaacact ggatatcaaa atcctctcca tctttcccga
      601 tgaattggac taatgatacc gatttttgtg gaaacaaaaa ttcagaatat ttttctggca
      661 aattttgcac aaagcgataa tgctcaagaa tatattgccc ccgggaccag gctcgccccc
      721 ctttatgcaa ataaggtcga tgaattcttg ctggcaacgt gggttgtatt gccagaatat
      781 cctcaatgca ctgcaaggaa cgcagttcgt aataatatcg aatactggca accggattta
      841 tagcacatcg caaaatgtac atgaaccgga attttctgtg ttcccacttc cccgggagtg
      901 aatctgagga aaaaacaatc cttttaagga aaccgataaa tgataagtta cttacagaaa
      961 acatattgtt acttgtgctt cgctaatgtg agtattttta acgcagtttt attaatatca
     1021 aaccgctctt ctatatcctg ttttgcctgg cgagccatct gataaaaacg cccagggtca
     1081 ttaaaaaaat cattcagggc gcacatccat gcgtgcttat tacctgctgg taacaatgtc
     1141 ccggtctggt tattctgaat aacatcaggg attccaccaa tctgactggc cagtacaggc
     1201 acagaaaatg ccgatgcttc tgccagtacc ataccaaacg attcgttttc tgaaggcaga
     1261 accaccagac tggcaacccg atatacgggg gcggcaggaa aaacattgtc agcaataaaa
     1321 acatcatcat gcatgcccat actgtcaatc tgatactgta aatgctcccg cagttcaggc
     1381 gaaccagacc ccacaatcaa ccaacagaac tgtcgtccat tcatttttaa atggaacagt
     1441 aaattcaaca taaattcgtg ccctttttcc gggcgcaaca ttcctacctg agcaataacg
     1501 gggcatcccc ttcgggacgc cagccaagaa agaacatttg gaggcaaact gtttcgggaa
     1561 tcaacgtata atttctggaa atcaaagcct ggaggcacaa cagtaacccg ggttcgacat
     1621 ccttcctgct ccagatgtgt cttcatactt gttccgggaa caatcacctc atcgcaaaaa
     1681 tgatttattg aaaaaacttt tgtttttcgt gtcaaatatg tcttttgcct gataattctg
     1741 aatggatgtt tcctagtgaa taaccgtact aaaccaacaa tattgctatc atgcccactg
     1801 tgacaaacga ttgcattggg ctgaaaacca tgaactattc cgagtaatct ccatgcagta
     1861 gggatgtgaa gactgtttcg aaataacgcg aatgtgatat caatccccaa tttactggct
     1921 tcaaaagcaa ttttgctgtt ttccctgcag acaagcagaa ctgaatgccc cattttcttc
     1981 agggccttca tttgagcaac agcctgtaat tcctgaccac ctatattcgg tgatgattcc
     2041 gtaaatagta tattcattat taatcatccg gtaatcttgg ccccttatga aaggccagca
     2101 acgatgaaaa aaacggggtt gtataataaa aaagtcgtct tttcagccat gcgcaacttt
     2161 cccgttcttt ggtgcttatt cttcctatcc gtgtagttcc t
//
LOCUS       DAZPFP010000323         2193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPFP010000323 DAZPFP010000000
VERSION     DAZPFP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.329"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..302)
                     /locus_tag="ACHUR4_004100"
     CDS             complement(<1..302)
                     /locus_tag="ACHUR4_004100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1457428.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGT"
     gene            complement(299..712)
                     /locus_tag="ACHUR4_004101"
     CDS             complement(299..712)
                     /locus_tag="ACHUR4_004101"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR1457429.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(746..2191)
                     /gene="gadC"
                     /locus_tag="ACHUR4_004102"
     CDS             complement(746..2191)
                     /gene="gadC"
                     /locus_tag="ACHUR4_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR1457430.1"
                     /translation="MAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFA
                     AIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLT
                     FGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGII
                     GTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEM
                     NAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDM
                     LFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGM
                     GHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQ
                     PDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLL
                     IPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
BASE COUNT          743 a          433 c          403 g          614 t
ORIGIN      
        1 aaagttccta tatttgagga gtttaataaa tttagctgtc ccaagtgatt tttaatagag
       61 atagcctgat catgtgcagg attaataaat tttatatccg gtgcaactgc ttcaaaaaaa
      121 ttctgaacaa ttggatagtg agtacagccg agaataattt catttactgg atggagggaa
      181 attaaatggt taacatgttt tttgatgtca ttcaagattg caggagcatt aagattacct
      241 tcctcaatta acattgcaag atttttgctt ggttctccaa atacatttat tgttgaattc
      301 aattttttat tagctctttg tgacaccccg ttttaatagt aaactcggta gcgataatac
      361 caacattttt atattgctgg cgaatcacat cttctactgc aggttcaatg atggataaga
      421 taggaaacgg aaagctctta gcatatccgc tgtattcttc ggattccaaa gttgaggata
      481 tagtattaca tgcgacagca actagtttga cattccttga ctgtaaaaaa gtcagcattt
      541 ttttagtcaa actgatgata tcatcggcag aacggttgcc ataaggtgca ttcatattat
      601 ctccacaata gataatatct tcaccaggaa gcaaattaat tatttccttt accgatgtca
      661 atccaccgac acctgagtct attacaccaa ttggagattc agcactaaac atatttaacc
      721 tcatcttttg tagcttatgg aaaatttaat tactagttct tttttcccaa gaaaatggtt
      781 cgaaatcaga gtttggatct ttccagtcag attttcggaa ggcgtaaatt aatattggaa
      841 ttaataaaaa tagggcaact aatactaaca aataaagaac atagctggtt ggactaccag
      901 ttgggatttg ctcgggcgga ataaaactta atacaaaagc cagaaaagac cctaagaatc
      961 caagtgttgc ccagaaaaat aaagcagggg ctcgatatgg gcgttttgta tcaggttgag
     1021 aatgtcgtag atagatagca ctagcgaaca tgagcatata cataactaaa tagagcataa
     1081 ctgttaactg tgataaaatc tgaaaagctg cttgaactga aggcattatc acaaaagtaa
     1141 cagaaagaac tgagacaatt ataccttgta caaacattag atggtgcccc attccatgac
     1201 gattggtttt ttggaaaaac ttaggtaaat aaccagcttt cgcgatcgct aatacacctg
     1261 tattaggccc tgcaatccaa gtaaccaccc cacccagtac gccaattgct agcataaatg
     1321 ccatcacaga gccaagccat ggcactccag cccattcgaa cagcatatcg taggcaacca
     1381 acaaactttg agttagacta attttatctg taggaataat aaaggcgacc cctaaagtac
     1441 tgagcaggaa tattgcaata gtacctatag aagcgatggt aattgcaatt gggtattttt
     1501 tagttggatt atctaaatcg ttaacatgga ccgcattcat ttccatccct gcataagcaa
     1561 gaaatatgct ggcggcaaga acaatattct ggaagttagc tatattaggg aaaagatcac
     1621 cccaacctaa tgggatcacc gctggtttac cgctgaacca cagataggag aaaccaagaa
     1681 taacaaggat aatagctgga ataattgtcc ctataatacc gcaccactta gatacctgcg
     1741 agaaaatacc aacgccacgt aatgcaacac aaatagctcc ccagtaaaca aatagtacga
     1801 aaaataaaat ataccattta ttactggcaa ttgattctgc cacccccata tcaacatcaa
     1861 taaatgcaac cgagactgca ccaaatgtca gtactgttgg aaagtagggg atgacttgta
     1921 gccaggtcat aagcattcct acaaaacccc agcgagcccc aaatgcttct cctatccatc
     1981 gaaatgcccc ccctttttgt ggatatgttg tagctagctc agccgcaaca aaggcaacag
     2041 gtactaaaaa cataattgca gcaaaggtat agtaaaaaat ggcacctaag ccgtattctg
     2101 cttcagaagg tataaatcgt agacttacta cagcagttat attcatgata gcgagtgtcc
     2161 ccactgtcat tttcttactt ttgggtgcga tat
//
LOCUS       DAZPFP010000324         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPFP010000324 DAZPFP010000000
VERSION     DAZPFP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.330"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHUR4_004103"
     CDS             complement(220..384)
                     /locus_tag="ACHUR4_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457431.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHUR4_004104"
     CDS             1286..1570
                     /locus_tag="ACHUR4_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1457432.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHUR4_004105"
     CDS             1558..2043
                     /locus_tag="ACHUR4_004105"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1457433.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZPFP010000325         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPFP010000325 DAZPFP010000000
VERSION     DAZPFP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.331"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUR4_004106"
     CDS             <1..203
                     /locus_tag="ACHUR4_004106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457434.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHUR4_004107"
     CDS             293..694
                     /locus_tag="ACHUR4_004107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457435.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHUR4_004108"
     CDS             696..1301
                     /locus_tag="ACHUR4_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR1457436.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHUR4_004109"
     CDS             1288..>2183
                     /locus_tag="ACHUR4_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1457437.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZPFP010000326         2178 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPFP010000326 DAZPFP010000000
VERSION     DAZPFP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.332"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            125..1207
                     /locus_tag="ACHUR4_004110"
     CDS             125..1207
                     /locus_tag="ACHUR4_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1457438.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1696..2178)
                     /locus_tag="ACHUR4_004111"
                     /pseudo
     CDS             complement(1696..2178)
                     /locus_tag="ACHUR4_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725250.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          640 a          449 c          438 g          651 t
ORIGIN      
        1 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
       61 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
      121 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
      181 gtctgctcag gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa
      241 agttaatgct aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt
      301 tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg
      361 ggaatatgaa ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg
      421 tcttgccttc gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta
      481 cggtgtagca tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac
      541 ctggacccaa acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa
      601 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa
      661 tgaccgcact gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga
      721 gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt
      781 tatctacggt aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg
      841 tctgaaatat gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat
      901 gactgttttt ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca
      961 atatcagttc gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga
     1021 cttgggtgcg tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta
     1081 cttcaacaaa aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga
     1141 tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca
     1201 gttctaatct gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt
     1261 ggcgctctca agccactttc cttagaagca ctatcttgct tctgactata taaattaccc
     1321 tttattttct gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt
     1381 atcaattaga tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca
     1441 tccctttaat tatcccctca acctcttgta aatttcccac tcttgagctc cacatagccc
     1501 aaacgccgga tgcaaaaaaa atcatacgtg catctctgaa attgtgatgg aggaagtgag
     1561 tatcaattat atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag
     1621 gctccaatag gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc
     1681 cttaataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa
     1741 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
     1801 ctcgctgatg ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt
     1861 cgtccaatat actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa
     1921 cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata
     1981 gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat
     2041 taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg
     2101 ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt
     2161 tctgaggtaa gcagaaac
//
LOCUS       DAZPFP010000327         2177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPFP010000327 DAZPFP010000000
VERSION     DAZPFP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.333"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUR4_004112"
     CDS             <1..187
                     /locus_tag="ACHUR4_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1457439.1"
                     /translation="KSRTTEFLFLILFIVLMTPISPQIFVWIIGKIIEPVIELYNDVV
                     WASFNTLHNKINPYKES"
     gene            189..554
                     /locus_tag="ACHUR4_004113"
     CDS             189..554
                     /locus_tag="ACHUR4_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1457440.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            551..1216
                     /locus_tag="ACHUR4_004114"
     CDS             551..1216
                     /locus_tag="ACHUR4_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457441.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1216..1581
                     /locus_tag="ACHUR4_004115"
     CDS             1216..1581
                     /locus_tag="ACHUR4_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1457442.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2036..>2177
                     /gene="hokD"
                     /locus_tag="ACHUR4_004116"
     CDS             2036..>2177
                     /gene="hokD"
                     /locus_tag="ACHUR4_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1457443.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAY"
BASE COUNT          589 a          460 c          586 g          542 t
ORIGIN      
        1 aaagagtaga acaacagaat tcctgtttct cattctgttt atcgtgttga tgacaccgat
       61 atccccgcaa atttttgtct ggataatcgg aaaaataatt gagccagtta ttgaattgta
      121 taacgacgtg gtatgggcgt cattcaacac actgcacaat aaaattaatc cgtataagga
      181 aagctgatat ggcactgacg aaaaaacaac gtgcagaact gcgcatgaag ttcggtggtc
      241 gctgcgctta ttgcggctgc gaacttggcg aaaagtggca tgcagaccat gtaaaaccgg
      301 tcattcgttt tgatggaaat atgcttcacc aggaacgtga cgatatatcc aacatggtac
      361 cagaatgcca cccatgcaat ctgcacaagc attgcagtag cctggaagat tatcgacgaa
      421 ttatcagtga tggtcgtcgt gaattccttg cgtccgggaa aggcaaagcg ctggttcgta
      481 tgggattggt tgaaatgaaa tctgacccgg ttgtgttctg gtttgaaata tatcaagaag
      541 gggctacggc atgactacta ttaccaaaga gcgactgcaa tggctggcta acatttctgg
      601 ccgcgatgat attgacgata tagacggcgg tgaaattcgt gaactggcgc ttatcgctct
      661 ggcatcactg gaagcagagc ctgtagcgga atgcattgtt gaagatgggg gcatgtgtat
      721 tgatgggttc ggtgagtatg tgggtcactc gctgcctgat ggaatgcatg agctttatgc
      781 tgccccgcga atgcgacaac ctgatggatg gaaagccgta gctgtagcgt ggaaggtgac
      841 gtttactcaa gttgaccagg aatctaatac gttcactgct atatattttt acaaagcgga
      901 agttgagcgt tgggtacgac tgcataaagc atgtgatttt cgggcagata taacaccgct
      961 ttacgcagcg ccggcagtgc cggttgcagt aaacgacgac atggcttacg cattccatca
     1021 cgcactgtca gattcatcgc ttggctctga tgaaatcgaa gaaattaaaa ccggtttgcg
     1081 tgctgccttt gccaacgtca ctatccaacc agagccgata gtgccggatg aaatcgggcc
     1141 aaacgatagt aatacgtttg attatgttga tggctggaac gcctgccgcg ctgccatgct
     1201 taagggagat aaataatgat taatcgaacc aaactggagc acattctcga atatgccagg
     1261 cagcagaaat gtattgggca actttgtaaa attccaccag gagatatggt tgaaatcgtg
     1321 gaaatggcca tgcgtaaggc tggcaactct ccggtaactc tggctggctg gataagctgt
     1381 agtgatgcag ttcctgctga atattgcgat gtgattcttc tcgatgatct cgggaatgta
     1441 ttccccggtt cctgggataa ggttttttgc cccactcgtg gcgggaataa gatggctttt
     1501 gtggacaaag acggcgttga agtagagagc tcaactcact ggatgccgct accggaacca
     1561 ccgcaggagg tgaatcaatg acctggcctg aggcattcac cacggtagga attgcgatgg
     1621 cggtggcgct ggtggtgtat tcgatttgcc gttggggtta acaaacaaaa accccggatt
     1681 gatggtccgg ggtttttgaa ggaaacaaaa cagaaacagc aattgccgtt acctgttgtt
     1741 accatggcaa gtaaacgtat ctcaggcgag cgcattgcgc cgttctgacg cagataaatt
     1801 agcctggata gaaggtgctg gcaataaaaa atagcgtttt cttatcggta tcggtaagat
     1861 tgctgcgggt gcttgaggct gtctgcctcg ggcatgccac tgtaaggcag acagagaaaa
     1921 gccccagtta acattacgcg tcctgcaaga cgcttaacat taatctgagg cccaatctat
     1981 gtctcacaaa tgtaggttag cctcttacgt gccgaaaggc aaggagaagc aggctatgaa
     2041 gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc
     2101 actggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc
     2161 tgtcttcaca gcttacg
//
LOCUS       DAZPFP010000328         2176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPFP010000328 DAZPFP010000000
VERSION     DAZPFP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.334"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHUR4_004117"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHUR4_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..>2176
                     /locus_tag="ACHUR4_004118"
     CDS             362..>2176
                     /locus_tag="ACHUR4_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1457444.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIESSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQR"
BASE COUNT          563 a          476 c          604 g          533 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgc
//
LOCUS       DAZPFP010000329         2154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPFP010000329 DAZPFP010000000
VERSION     DAZPFP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.335"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            310..1500
                     /locus_tag="ACHUR4_004119"
     CDS             310..1500
                     /locus_tag="ACHUR4_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR1457445.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(1890..>2154)
                     /locus_tag="ACHUR4_004120"
     CDS             complement(1890..>2154)
                     /locus_tag="ACHUR4_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR1457446.1"
                     /translation="GNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKE
                     QIEKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          602 a          447 c          478 g          627 t
ORIGIN      
        1 cagtaccacc gatagtatcg atatcaaatc agcctgcggc cgcgataact ttcgtaacat
       61 tctgccagcg gctttgttga ataaatcagg ttcaagataa tcgtctctgc atcaggttag
      121 agtttcaggc aatacatcct ctttctggca tacctgccac tctcgcacca tggtcatagc
      181 ctttacgatc tgcatactca ccagaccaga gcccgctccc ttttaggctg aggaatcccc
      241 agaaaagaag acaggcatag agttttgtga tctactgatt gcgctaacaa attcaatgag
      301 atcacctcta tgcctgcccg taaagtatgc cagacttttt tccgtaatgc tttagcttcc
      361 acgcatcagt atcgacaaaa tgccattatt gattctgctg ctgctttagt cggtggcgca
      421 tctctttctc ttaccagcat tggccggcat ttacccggtc ctgctcgcgt gaaagataaa
      481 ataaaacgcg ttgactgtct gttgggtaat aaacgacttc ataatgatat ccctctgata
      541 ttcaaaaata ttacatccat gatgacaaat aaactgtcgt ggtgtgttat cgctgttgac
      601 tggagcggtt acccttttca ggaatatcat gtccttcgtg ccagcctcct ctgcgacggt
      661 cgctctatcc ccctgatgag tcaggtcttc ccgtcaaaaa aaaataacga ggcggtggaa
      721 atcgcttttc tggatgcgct ttccggggcc atttctcccc ggactcgtgt tgttattgtt
      781 actgatgctg gttttcaaag tgcatggttc cgtcatatca aatcacaggg atgggatttt
      841 attggtcgta tcaggggggt agtgaagttc aggctggata gtgacaaaga taagtggctg
      901 gacataaaaa tgtgcagggg gagttcagag gcaaaatacc tggggacggg aaccctggcc
      961 cggaaaaaac gctcgcagtg tgaaggacat ttttatcttt ataaacattc acctaaaggc
     1021 agaaaaagca ggcgtgccag agggaggccg ggcttaccaa caacggaaaa agaacaaaaa
     1081 gccgcaggca gagaaccatg gttaatattc agcaatacag ctgagtttaa tgccaaaaaa
     1141 atcatgaaac tgtacagtcg aagaatgcag atagagcaaa actttcggga tgaaaaaagt
     1201 gaacgctttg gttttggcct tcgggcgagc agaagtcgga ggggagagcg ttttctggta
     1261 ttaagtctac tggttacact ggccagtatc gtactctggc tacttggtta tcattttgaa
     1321 aataaaggat ttcatttaaa gtatcaggct aatagtctca aaaaacgaag agtattgtct
     1381 tttctgacgc tggctgaaaa cgtcctgaga tttaaccctg aacttctacg acgagcacaa
     1441 cctgaaaaga tattgtgccg gttggccagc acttaccgaa gtatggtgct ggcttactga
     1501 tgaaaatttt ctggggatcc ctcagccttt cggggaggaa tgagggctct gatattcttt
     1561 tgccgtagtt catcgtgaaa ccgtcggatg tcatttggcc tgagtgaccc atgcctccca
     1621 aaagcagagt cgctagtgtt atgtaattag gcggttaaac ttcaattctc agtgttaagt
     1681 ttgggtaaac gcttgtaaat ggtattgcta attttttaat tgactaatat tagaaacggt
     1741 tagtgtgatt gtatatagat atttgagtgt aaggtattta aaggggcatt cagcccctcc
     1801 gtctatttgt gattgcatcc attgatgtgt gtattatttt gagcttgaaa aagggaggcg
     1861 ctctttaata ataaataaat ctatcaaagc taaatattaa atggaagcca cccttttttc
     1921 tctgatagtt ttattatcac caaaccattt atttcagcta tcgaatctgg attcggccag
     1981 gaaggtaaac tcttcacaat tggataaatc ttctctattt gttctttatt tgctggtttg
     2041 caattacaga ttccatagta attcataaat gcaaccatcc taaaatagtt gccaccatcc
     2101 caccaaaaaa aagattttcc aaaagtgtct gcgctcatgg agagcatatt tcct
//
LOCUS       DAZPFP010000330         2135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPFP010000330 DAZPFP010000000
VERSION     DAZPFP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.336"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUR4_004121"
     CDS             complement(<1..105)
                     /locus_tag="ACHUR4_004121"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1457447.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGT"
     gene            complement(65..400)
                     /locus_tag="ACHUR4_004122"
                     /pseudo
     CDS             complement(65..400)
                     /locus_tag="ACHUR4_004122"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(397..930)
                     /locus_tag="ACHUR4_004123"
     CDS             complement(397..930)
                     /locus_tag="ACHUR4_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1457448.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(996..1625)
                     /locus_tag="ACHUR4_004124"
     CDS             complement(996..1625)
                     /locus_tag="ACHUR4_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457449.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1629..1844)
                     /locus_tag="ACHUR4_004125"
     CDS             complement(1629..1844)
                     /locus_tag="ACHUR4_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1457450.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          568 c          471 g          550 t
ORIGIN      
        1 cgtcccggat accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat
       61 tgcccatgcc ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga
      121 cgagcacccg accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat
      181 cagctaactc cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca
      241 tgtcagtaat ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt
      301 tgtaggtaat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
      361 agataaccag agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc
      421 taatgcgctt tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg
      481 gcaatcgcgc ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc
      541 accggcattc agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata
      601 ggggcaaaat gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg
      661 ctccacccat gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt
      721 cgacagcttc attcccggta tgacaggctt accatccacc attgtggcac cacgacagat
      781 ggtccatatg ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag
      841 aaactggtca agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc
      901 cgacaggccg tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa
      961 taacgagcca acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc
     1021 aggctctttc ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca
     1081 tccatgtctg caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg
     1141 caacggccaa tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga
     1201 ttaataattt tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg
     1261 acgaactcac cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc
     1321 ggtgcagacg caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg
     1381 acgtactcag gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt
     1441 tcctgcaaaa acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc
     1501 gcataatgct catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt
     1561 ttattaagct cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc
     1621 ttcattattt actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga
     1681 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
     1741 tgtgatggcc tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
     1801 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
     1861 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
     1921 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
     1981 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
     2041 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
     2101 gggatttccc atcatcgcag actgaaagac tctga
//
LOCUS       DAZPFP010000331         2094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPFP010000331 DAZPFP010000000
VERSION     DAZPFP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.337"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(190..409)
                     /gene="ymgF"
                     /locus_tag="ACHUR4_004126"
                     /pseudo
     CDS             complement(190..409)
                     /gene="ymgF"
                     /locus_tag="ACHUR4_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(541..2064)
                     /locus_tag="ACHUR4_004127"
     CDS             complement(541..2064)
                     /locus_tag="ACHUR4_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1457451.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          630 a          462 c          442 g          560 t
ORIGIN      
        1 taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt atttcggtga
       61 tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat aattcctttc
      121 cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg acagtgagaa
      181 actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat cgattaacca
      241 gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc acttctccca
      301 gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata aatgcaatgg
      361 aaaagatgat atagagagtg aaataatcca gattattact attattcatg ctactaatgg
      421 caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa tagtaacaag
      481 aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac tgatcaattt
      541 tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca ggtcgatgta
      601 tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt ggttcagata
      661 gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa gtttacgcgc
      721 cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta cgcggccaac
      781 gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag aaagccctga
      841 gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca gaatgttcaa
      901 ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt ccagaacaag
      961 gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga caaacgtcgg
     1021 cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg gcagtttact
     1081 cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa gcggaacgat
     1141 taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc caccgtgagg
     1201 ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac catttaccac
     1261 aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga tctcttcagg
     1321 cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgctgg cgattaacag
     1381 aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa agagtggcgg
     1441 cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg tatagggata
     1501 atgtccatta agcgtcatca catcagccgt tggtccaata atgtcatccg ccacgcgtaa
     1561 tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt gatcacttat
     1621 cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag agcaggatag gtaatctatt
     1681 aacggtgtcg attgccggag ccaacagtaa attactgtcc gggacaacag gaatacgaga
     1741 cagcaggacc cgattcccag gcagggatgt acaccagact attccctgtt tgagaatgat
     1801 gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt gctcctcgag
     1861 gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc gcctgtgacg cttcttcaag
     1921 tatatgattg atattgttgg cggcatatct tgctcccgcc aggtactctg cttttgcgga
     1981 ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata tcgcaacgag
     2041 gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa taca
//
LOCUS       DAZPFP010000332         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPFP010000332 DAZPFP010000000
VERSION     DAZPFP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.338"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHUR4_004128"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHUR4_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1457452.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUR4_004129"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUR4_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1457453.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZPFP010000333         2090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPFP010000333 DAZPFP010000000
VERSION     DAZPFP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.339"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHUR4_004130"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHUR4_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHUR4_004131"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHUR4_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHUR4_004132"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHUR4_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          505 c          632 g          437 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagcagctt
//
LOCUS       DAZPFP010000334         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPFP010000334 DAZPFP010000000
VERSION     DAZPFP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.340"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUR4_004133"
     CDS             complement(60..1025)
                     /locus_tag="ACHUR4_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1457454.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUR4_004134"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUR4_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457455.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          549 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga acccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPFP010000335         2084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPFP010000335 DAZPFP010000000
VERSION     DAZPFP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.341"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(127..861)
                     /locus_tag="ACHUR4_004135"
     CDS             complement(127..861)
                     /locus_tag="ACHUR4_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1457456.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            complement(861..>2084)
                     /locus_tag="ACHUR4_004136"
     CDS             complement(861..>2084)
                     /locus_tag="ACHUR4_004136"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1457457.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
BASE COUNT          583 a          539 c          448 g          514 t
ORIGIN      
        1 cttatttttt tccaggtttc acagctacaa acccgtggca aaacatagca cccatctatt
       61 actaacctca ccacgcatgc tcagcgattt cgtcatcgaa aaagagagtt atctatgacg
      121 catcaattat actttttgct ttgttcgcat attgttacat ctttcatacc ttgacgcatc
      181 tcttcccaac gcgggtatcg cctgttgtat aagttgagca ttacgtacta accagacgta
      241 ctcactttta ttcctggtgc ctaatcgttc tcgaattgcg gactcatggg cgctaacggt
      301 cttgaggcca ataccacaga tttctgcaat tttacgtgtg ctaatcccct tcgcgcggag
      361 taaaagaacc ctccattgca ctcttcccag gcgttttata aacaaaggac caggcaaaca
      421 atgagcattt aacaccttcc gtaatgaaga taataaaata tccacttttg cactcccatg
      481 agtaagccat actcgggaaa gaggcccgat atcattcgtc attccttcag gaatagcatc
      541 gctaataacg agttgccgca tcagacggta tcgacgacaa accttcacaa tatgtccggc
      601 taattcaggc aaaccatcgt gacgcatttt tacccatatc acgatgctac tgtcagtcag
      661 aacattcata tcagtgggta aggaagtgaa aacctgaagt ttcactcccc caacacgctt
      721 gagcaaactg cacacgccgc aaatggcaaa aagatccagg ccaatcaccg tgacatttgt
      781 ccatgtggta ttttctggcg agagaatatt acagttgatg tttgtgcttt ctaatttctt
      841 atacgtcaga tcgttaacca ttagtttccc ccgcgagtaa aacgttcgtc atttacaaat
      901 acccgagctg cgcgttttct tattttttca gctgtcagat aatcaccgcg cctgtgcgca
      961 atgaatatca ttgcagcgct tacgtctgca tcattgtgga ttgccaacca tttatttccc
     1021 cacacagtga aaagagacat gctttccgta tcgcgaacga accccggttt attcgcaata
     1081 agcagatgct gatcgcgctc aagacgttct gcctgcgtca ggtatttcca gctatgttcc
     1141 ggaaggcgag ggataaatcc ttcgttcata tcagcttcag ctctggtcag ttcccgctgt
     1201 gcatcaaatc ccgctaccag gatgaccagc gcggcggtca taatgagcac aatgacaggc
     1261 agaataccca accggactgt tttaggtata cgtacctgat tcatcacgtt ttccggcatt
     1321 ccgatgcgga aaaggagcag caacagcatc aaatggggaa cagacgtata gagaggatat
     1381 tcggtcatca tatgaatgac taaaggcaac cccattaagg caataaacca cttttcacat
     1441 tgatggtgcg cgttattgtt tgtcattttc aggcggcata tacacggcca cagccaaacg
     1501 cctgatagca gcaaaaaacc aaacaaggcg acgatgccac cttcagccgt tacgaacagc
     1561 agttcgttat ggggatgcgt agccgtatca ctctctatgt ttgtcacgcc cacatcctct
     1621 aaggcttgcg ggaaggtcgc ttcaaagctg cctaaccccc aaccggttaa ggggcgctgt
     1681 tgaataagac ttatggcacc tttcagaata cgtatacgct ggtcattcga tgatgaatgc
     1741 gtaaagtcag ccgttgtctc ccctggcatg gcatagtttg agataccgac acaccagcac
     1801 agcaaggagg cgacgaagat caagcgcaac agaccgctaa ggggacgtcc gcgccagcgg
     1861 ggtctgtccg cgagagcggc cagcacgatg acgcaaaata aaccaagaag gccgacacgg
     1921 ctttgtgtaa ggactaaagc tgtagacaga gggtataacg acaacaaaca tatcaacgtc
     1981 agagaacgag tacgcgccgt taaagccaga tacaacgcta cgccgcaacc tgttgccagc
     2041 cagctcgcaa gaacatttcg ctgctggaaa atgccatacg gaaa
//
LOCUS       DAZPFP010000336         2079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPFP010000336 DAZPFP010000000
VERSION     DAZPFP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.342"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(435..1373)
                     /gene="lrhA"
                     /locus_tag="ACHUR4_004137"
     CDS             complement(435..1373)
                     /gene="lrhA"
                     /locus_tag="ACHUR4_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1457458.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          594 a          482 c          423 g          580 t
ORIGIN      
        1 aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat
       61 aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt
      121 gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg
      181 cttttgttat tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt
      241 agcaggtgat ttttttcagg cgattatttg tgcattcggg acgtgaatct ctggtgattg
      301 aaaaataaac agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag
      361 ccgctggggt ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat
      421 ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga
      481 gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc
      541 tgggccagct cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc
      601 ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc
      661 cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg
      721 acataagcca gtcgccatgg aatatctact ttattaagcg tcgccagcac catatcgcga
      781 aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat
      841 tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa
      901 ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag
      961 gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt
     1021 aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc
     1081 tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg
     1141 gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg
     1201 aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga
     1261 gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat
     1321 gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat
     1381 cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc
     1441 atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata
     1501 tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag
     1561 aatatatctt cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg
     1621 cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt
     1681 catacgcgaa cacgtacaaa cagcaatggt gaagcggcat caagagcagg gatccgtcat
     1741 ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga
     1801 tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc
     1861 tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca
     1921 atagtttcac accttacccc cattgcgaca cctcggaaat catcacgctg ttaaactctc
     1981 cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca
     2041 cacaattatc ttacagataa aaaaaccaga cttgcaatt
//
LOCUS       DAZPFP010000337         2041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPFP010000337 DAZPFP010000000
VERSION     DAZPFP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.343"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            121..1098
                     /gene="intI2"
                     /locus_tag="ACHUR4_004138"
                     /pseudo
     CDS             121..1098
                     /gene="intI2"
                     /locus_tag="ACHUR4_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1286..1525)
                     /locus_tag="ACHUR4_004139"
     CDS             complement(1286..1525)
                     /locus_tag="ACHUR4_004139"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1457459.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          590 a          443 c          433 g          575 t
ORIGIN      
        1 agcattaaat taaaactcta ccggtaaaca agcatctcta ggcgtaaaaa acaggcactt
       61 ttttaaatcc cagctagaat aggctgtata ggcagacagt tgcaagacaa ggagcgtttt
      121 atgtctaaca gtccattttt aaattctata cgcacggata tgcgacaaaa aggttatgcg
      181 ctgaaaactg aaaaaactta cctgcactgg attaagcgtt ttattctgtt tcacaaaaaa
      241 cgtcatcctc agaccatggg cagtgaagag gtcaggctgt ttttatccag cttagcaaac
      301 agcagacatg tagccataaa cacgcagaaa atcgctttaa atgccctagc ttttttgtac
      361 aacaggtttt tacaacagcc gttgggcgat attgattata tccctgcaag caagcctaga
      421 cggctaccct ctgttatctc tgcaaatgaa gtgcaacgca ttttgcaggt tatggatact
      481 cgcaaccaag ttatttttac gctgctgtat ggtgcaggtt tgcgcattaa tgaatgcttg
      541 cgtttgcggg ttaaagattt tgattttgat aatggctgca tcactgtgca tgacggtaag
      601 ggtgggaaaa gcagaaacag cctactgccc acgcgcctaa tcccagcaat aaaataactc
      661 attgagcaag cgcggcttat tcagcaagac gacaacttac aaggcgtagg gccatcgctg
      721 ccttttgctt tagatcacaa atacccttct gcttatcgac aagcggcgtg gatgtttgtc
      781 tttccctcca gcacgctctg caaccacccg tataacggca aattatgccg ccatcatctg
      841 catgactccg ttgcgcgaaa ggcattgaag gcagccgtac aaaaagcagg catcgttagc
      901 aagcgtgtca cttgtcatac atttcgtcac tcgtttgcta cgcatctatt acaagcgggg
      961 cgtgatattc gcactgtgca agaactctta gggcataacg atgttaagac cacgcaaatc
     1021 tatacgcatg tgttgggtca gcattttgcc ggcaccacca gtcctgcgga tggactgatg
     1081 ctacttatca atcagtaaaa gtagataaat atctttatgt agccctgagt agcccctgat
     1141 actttttatg gtgaaaaaga taaacaatca tagaggggat aaatatgagc cagttctacc
     1201 attgttcaga taagtttaag caagatactg tcaatcacag tgaccattca tggtcattac
     1261 acttcctcca gttgccagac aatgcctaac ataaaaacgc aaaagcccaa aagtcagagc
     1321 ttaggtgttc taggcgtttt ctaagcgctg aagaaatacg tcttaccgta gattgcgtgc
     1381 gtttctgaag cacattttcc ttatacatgg ctttatccca ggcagcttca tcaagctctt
     1441 ggagtaacaa gccagtaata atgcgacact ctcgggtttg tagcgagccg ctggtgagat
     1501 cggagttata gcggaaaggg gtcatttgta attatttgtg ggtggaggat agttaactta
     1561 ttattgtagt tggaagaggc tgctttccaa acattagcac ctcgtaatgc ttgttactgt
     1621 atattaattt atgctcttag cgcatgttaa tgggctatta caatgcataa tgtccacatc
     1681 aagataactg tggcttaaca cctgtacaag cgaaaaaaat caatcacctg tccggaaata
     1741 cgggatcact gtaaagtgtt ggtgaggcag tctaggaggg ggatttaatt tgggatactg
     1801 atgaaaggat tatgatcagt tggatatagg atcgtactaa gctgagcggc aggttagcta
     1861 gattagttaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     1921 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     1981 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagctgcc
     2041 g
//
LOCUS       DAZPFP010000338         2040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPFP010000338 DAZPFP010000000
VERSION     DAZPFP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.344"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHUR4_004140"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHUR4_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZPFP010000339         2034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPFP010000339 DAZPFP010000000
VERSION     DAZPFP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.345"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..2034
                     /gene="sat"
                     /locus_tag="ACHUR4_004141"
                     /pseudo
     CDS             1..2034
                     /gene="sat"
                     /locus_tag="ACHUR4_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          746 a          357 c          401 g          530 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga agtgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcag
//
LOCUS       DAZPFP010000340         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPFP010000340 DAZPFP010000000
VERSION     DAZPFP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.346"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHUR4_004142"
     CDS             complement(<1..1824)
                     /locus_tag="ACHUR4_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR1457460.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHUR4_004143"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHUR4_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457461.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZPFP010000341         1994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPFP010000341 DAZPFP010000000
VERSION     DAZPFP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.347"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1068
                     /locus_tag="ACHUR4_004144"
     CDS             <1..1068
                     /locus_tag="ACHUR4_004144"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1457462.1"
                     /translation="FFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKVSS
                     LMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEA
                     SVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPIS
                     DEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKRTVK
                     ESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYIL
                     SIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISY
                     IGIYHKTQLHLADALTDAAIN"
     gene            1289..1630
                     /gene="symE"
                     /locus_tag="ACHUR4_004145"
     CDS             1289..1630
                     /gene="symE"
                     /locus_tag="ACHUR4_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR1457463.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(1677..>1994)
                     /locus_tag="ACHUR4_004146"
     CDS             complement(1677..>1994)
                     /locus_tag="ACHUR4_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR1457464.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          618 a          449 c          397 g          530 t
ORIGIN      
        1 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
       61 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
      121 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
      181 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
      241 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
      301 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
      361 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
      421 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
      481 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
      541 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
      601 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
      661 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
      721 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
      781 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
      841 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc caaaacttaa ttctgacagt
      901 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
      961 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     1021 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     1081 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     1141 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     1201 gatgtatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     1261 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     1321 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     1381 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     1441 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     1501 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     1561 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     1621 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     1681 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     1741 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     1801 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     1861 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     1921 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     1981 agatgatcga ataa
//
LOCUS       DAZPFP010000342         1980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPFP010000342 DAZPFP010000000
VERSION     DAZPFP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.348"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..662)
                     /locus_tag="ACHUR4_004147"
     CDS             complement(<1..662)
                     /locus_tag="ACHUR4_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR1457465.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUR4_004148"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUR4_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1457466.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004149"
     CDS             complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1457467.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          486 a          488 c          452 g          554 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
//
LOCUS       DAZPFP010000343         1975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPFP010000343 DAZPFP010000000
VERSION     DAZPFP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.349"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..206
                     /locus_tag="ACHUR4_004150"
     CDS             <1..206
                     /locus_tag="ACHUR4_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457468.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="ACHUR4_004151"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="ACHUR4_004151"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1457469.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          498 a          484 c          451 g          542 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatat
//
LOCUS       DAZPFP010000344         1971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPFP010000344 DAZPFP010000000
VERSION     DAZPFP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.350"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(766..1755)
                     /locus_tag="ACHUR4_004152"
     CDS             complement(766..1755)
                     /locus_tag="ACHUR4_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457470.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(1839..>1971)
                     /locus_tag="ACHUR4_004153"
     CDS             complement(1839..>1971)
                     /locus_tag="ACHUR4_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457471.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          535 a          460 c          491 g          485 t
ORIGIN      
        1 gattcgcttg tcacttctgc ctctgtcgcc tggcatgtgc cgcacgaaaa tataccagcg
       61 agcagaagag ttaatgcaga tttccctttc ataaaataat ccatcaatcg tttgttaaaa
      121 gcgtagataa ccacttattc aacaacttga aaataaggta ttttacctga gttgttacaa
      181 aaggattgca ttgcgtaaac gctttttatt tacaaaaaaa tggggaagtg ttacagcgat
      241 tgcgaaaagc ctgaagtgat tgaattaaga taatatagaa aatcagatac agcaaaaatg
      301 gcagattgat caggtgaaga taaattggag cgggcgaagg gagcctacga caaatcgaag
      361 acaaccagct aacctattgt ttcttaaaga caggtgaatc cagttggttg tagtgatagt
      421 cacacattta gagatagaag gtcaagactt gaagcacgta atattccctc taaaggaata
      481 gaactcacgg tctgactacc cggccctttt ggtctggctc aggtcaggct atgggggctt
      541 ttggctcgct ctttatatta gatgaggtct cacatttttc agtcaaaact catcgtctaa
      601 tagcccatag ccagaccacc agtcccagct ttagcaggaa gaccacgaga tagactttgt
      661 gtaggtccat ttggccctcc tactttctgt attcacaaac tagattacta gtacaaaata
      721 aaggccagac atccagttcg gaagtctgac caatagtcgt tattgttagt tagcttcttt
      781 agtgaagctc aggcgtttga ctacaggcag gacagcagta gccgagccag tacccatctt
      841 gatggtcgta gcagcttcag ctacaacaaa caccttgccg agaatctcaa ggtcgtgaga
      901 ctgggagtct ccatcacgtg aaaggaccac agtaatgctc tcgccatcgt gacggaagca
      961 accgaagctc agagccgtaa aacgcttagc catcatgaac acgccacggt cttcaccgtt
     1021 gtaataggcg acgtcagtcc ccagcatatc gacgatagcg tcttcaccac cgaagccctg
     1081 cttagcagcc agacgggaga gagccttgag gtgtgactca tgggcgataa tgccgtagtc
     1141 agaaacctca gaaccgaaag aagactccgt gttctcgtac agggcatcca tgatagcgtc
     1201 agccttagcg gtcaggctac cagtcagggc cggaagttct acggtcttca gcagctttga
     1261 ggactccagc acgtcaacca tcttagagtt aatggtacga atcacgttat cgcagatgtt
     1321 cagtgcgtcc tgctggagcg tcccctgagt gaaaccagtg gtcgacttct ggtcccaaag
     1381 ctttgtttgg tgagacacga ggatcgcctg ttcaatcggc tcaacggtga tttcagtgcc
     1441 agacggagcg gtcacaactt caggtcgctt accaccacgg acagggacca gcacagagcg
     1501 gtcagccaga gtgtgagcaa tgattggagt cggtaagctg ttacgggtcg gcaggacttc
     1561 tacagggaac gggatgttat cttgagcgtc tttaagtaca ccgttcagca tagccttaac
     1621 agtcttagct aactggaggg catagacctg agggtcggcg ttagtcggca gaccttcatt
     1681 gacctgaatg ttgtcggtat cggtgatgcg tactggctca atgccaaact ggaaagcgtt
     1741 tttagtcata ttcattataa ttaatctcct tgagtcaaat acttgaagtc cctaacaggc
     1801 agtcagggac cgtgtgttct cagatgaggg acgcttgctt acaagctgtc catccagtta
     1861 ccgcctgact caaggtcagc gacgatagcg gcacgtgagc gaccagcgga atgagtagcg
     1921 ctgcggaggt tttcaccata ggtccggcgt tcagctttga cctgttcgcg g
//
LOCUS       DAZPFP010000345         1935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPFP010000345 DAZPFP010000000
VERSION     DAZPFP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.351"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUR4_004154"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUR4_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1457472.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHUR4_004155"
     CDS             complement(590..1255)
                     /locus_tag="ACHUR4_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1457473.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1780)
                     /locus_tag="ACHUR4_004156"
     CDS             complement(1424..1780)
                     /locus_tag="ACHUR4_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457474.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="ACHUR4_004157"
     CDS             complement(1838..>1935)
                     /locus_tag="ACHUR4_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1457475.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DAZPFP010000346         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPFP010000346 DAZPFP010000000
VERSION     DAZPFP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.352"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHUR4_004158"
     CDS             complement(53..1702)
                     /locus_tag="ACHUR4_004158"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1457476.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZPFP010000347         1875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPFP010000347 DAZPFP010000000
VERSION     DAZPFP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.353"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            54..1469
                     /gene="tnaA"
                     /locus_tag="ACHUR4_004159"
     CDS             54..1469
                     /gene="tnaA"
                     /locus_tag="ACHUR4_004159"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR1457477.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1560..>1875
                     /locus_tag="ACHUR4_004160"
     CDS             1560..>1875
                     /locus_tag="ACHUR4_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1457478.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          507 a          432 c          457 g          479 t
ORIGIN      
        1 agatgtaata ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa
       61 actttaaaca tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta
      121 ctcgcgctta tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata
      181 gcgaagatgt gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta
      241 tgcaggcagc gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt
      301 tagccgagtc agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc
      361 gtggcgcaga gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc
      421 tggatcgcag caaaatggtg gcattctcta actatttctt tgataccacg cagggccata
      481 gccagattaa cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg
      541 tgcgttacga ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag
      601 ttggcccgaa taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc
      661 agccggtttc actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc
      721 cggtggtaat ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag
      781 cagaatacaa agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata
      841 tgctggcgat gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga
      901 aagacgacag cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag
      961 gcttcccgac atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt
     1021 atgacggcat gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg
     1081 atggtctgga agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg
     1141 atgccggtaa actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt
     1201 gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc
     1261 gcgatccgaa aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc
     1321 cgcgcgcaac atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa
     1381 aagagaacgc ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc
     1441 acttcaccgc aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta
     1501 gccactctct taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca
     1561 tgactgatca agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta
     1621 cagtaattgg tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct
     1681 ggggtgcctt tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat
     1741 tagaagcaaa tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa
     1801 taggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca
     1861 cttatgccta tatct
//
LOCUS       DAZPFP010000348         1870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPFP010000348 DAZPFP010000000
VERSION     DAZPFP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.354"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..884
                     /gene="hprS"
                     /locus_tag="ACHUR4_004161"
     CDS             <1..884
                     /gene="hprS"
                     /locus_tag="ACHUR4_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1457479.1"
                     /translation="NSIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSR
                     VPVEVNALPRELKPLGQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVT
                     LSQIRSIAEYQKTIAGNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENL
                     LDYLEYLSDEKEICFKVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLD
                     ANGSLNIDIASPGTKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATY
                     HYLSKHNVFRITLPQRN"
     gene            complement(992..1843)
                     /gene="hchA"
                     /locus_tag="ACHUR4_004162"
     CDS             complement(992..1843)
                     /gene="hchA"
                     /locus_tag="ACHUR4_004162"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1457480.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT          527 a          394 c          429 g          520 t
ORIGIN      
        1 ttaatagcat tataatttgc attgtcgcca ttatactgtg ctcagtatta agtccgttgt
       61 taatcaggac ggggttgcga gagatcaaaa agttgagtgg cgtaacggaa gcgctgaatt
      121 ataacgatag ccgagtgccg gttgaggtta acgcattacc gagagaacta aaacctcttg
      181 ggcaggcgtt gaataaaatg catcatgctt tagtcaaaga ttttgagcgc cttagtcaat
      241 ttgctgacga tctcgctcat gaacttagaa cgccaattaa tgcattactg ggtcagaatc
      301 aggttacgct cagtcaaatc agaagtatcg ctgaatatca aaaaacaatt gccggaaaca
      361 ttgaagagct ggaaaatatt tcgcggttaa cagagaatat actgtttctt gccagggcag
      421 acaaaaacaa tgttttggtg aaactggatt cgctttctct caataaggaa gttgaaaatc
      481 ttttggacta tcttgaatat ctttcagacg agaaagagat ttgctttaag gtcaagtgca
      541 atcaacaaat ctttgcggat aaaattttac tgcaacgaat gttatcgaat cttattgtta
      601 acgccattcg atattcacca gaaaaatcgc gtattcatat aaccagtttt cttgatgcca
      661 acggctctct taatattgat atcgccagtc ctggaacgaa aattaatgag cctgaaaaac
      721 tcttccgtag attttggcgg ggagataatt cgcgtcattc cgtaggtcag ggacttggcc
      781 tttctttagt caaagcgatt gccgaattac atggaggaag tgctacgtat cactatctca
      841 gtaagcataa cgtgttccga attacgttac cgcaaagaaa ttaacatcgg gcttcagtac
      901 gccataagtt aaccgtgaaa aaacgcagcc acgtcgtgag tattaacgtg gctgcgattg
      961 attatgcgct tacatcacaa tgtaacaggg attaactcgc gtaagctgcc agcatttcct
     1021 gcgccgccag tttacccaac gcattcgctg caaaaggact gtcgccggtg agacgtttac
     1081 ggtccttatg tactcgcccg gtgatgtcgt cattaatgat gttcatgccc attttcttca
     1141 actcttcgcc gaagtaccag gtgagatgac ccggcatata gccaatatct ggcgtttgtt
     1201 tgtctgcggc gtctgggaat gcgcaaatag aataaccatt cagtgggtta tcgctgtggc
     1261 gaagtgccag aaaagccgcc ggaccgtggc aaagggagat aacaaaacgg tcatttttga
     1321 ttgcccactg taaagcggca gccacgtcct ggctttctgg taaaccaata agtgcgccat
     1381 gcccaccagg aacaaagatt gctgcatatt cgctatcagc gttgaggctg gcgacaacat
     1441 ccgcgagttt cttcggatta cggaataacg atttatgctg ttcaaagaat ggcattactt
     1501 tttcatcttt gtgcggcata gcccagtatt caaacttggt catcagaccg gaaatggtcg
     1561 ccacttcgaa ttcgaaacct gcagcatgga gatgatacaa cggcagcaac gtttcaatcg
     1621 gatggttacc ggtcgagaac agttttccgt tatcggtcgg caaataacgt tcgtccgcgg
     1681 cgatcaccag aattttatgt ttaccgcgat acggttttgg atagtccacg ccatcaagat
     1741 cagagacggg actcgtatat tggctaagcg aatattctga agggaagaac gcattatctt
     1801 cagcaatatc gacctgcggg tttttacttg tttgaacagt catagtgtat tccttattgt
     1861 tgcttagtta
//
LOCUS       DAZPFP010000349         1856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPFP010000349 DAZPFP010000000
VERSION     DAZPFP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.355"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1223
                     /gene="ipaB"
                     /locus_tag="ACHUR4_004163"
     CDS             <1..1223
                     /gene="ipaB"
                     /locus_tag="ACHUR4_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR1457481.1"
                     /translation="KKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            1243..>1856
                     /gene="ipaC"
                     /locus_tag="ACHUR4_004164"
     CDS             1243..>1856
                     /gene="ipaC"
                     /locus_tag="ACHUR4_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1457482.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSK"
BASE COUNT          637 a          385 c          349 g          485 t
ORIGIN      
        1 aaaagaaaaa attaagccgg gaagaaatac aactcactat caaaaaagac gcagcagtta
       61 aagacaggac attgattgag cagaaaaccc tgtcaattca tagcaaactt acagataaat
      121 caatgcaact cgaaaaagaa atagactctt tttctgcatt ttcaaacaca gcatctgctg
      181 aacagctatc aacccagcag aaatcattaa ccggacttgc cagtgttact caattgatgg
      241 caacctttat tcaactagtt ggaaaaaata atgaagaatc tttaaaaaat gatctggctc
      301 tattccagtc tctccaagaa tcaagaaaaa ctgaaatgga gagaaaatct gatgagtatg
      361 ctgctgaagt acgtaaagca gaagaactca acagagtaat gggttgtgtt gggaaaatac
      421 ttggggcact tttaactatc gttagtgttg ttgcagcagc tttttctgga ggagcctctc
      481 tagcactggc agctgttggt ttagctctta tggttacgga tgctatagta caagcagcga
      541 ccggcaattc cttcatggaa caagccctga atccgatcat gaaagcagtc attgaaccct
      601 taatcaaact cctttcagat gcatttacaa aaatgctcga aggcttgggc gtcgactcga
      661 aaaaagccaa aatgattggc tctattctgg gggcaatcgc aggcgctctt gtcctagttg
      721 cagcagtcgt tctcgtagcc actgttggta aacaggcagc agcaaaactt gcagaaaata
      781 ttggcaaaat aataggtaaa accctcacag accttatacc aaagtttctc aagaattttt
      841 cttctcaact ggacgattta atcactaatg ctgttgccag attaaataaa tttcttggtg
      901 cagcgggtga tgaagtaata tccaaacaaa ttatttccac ccatttaaac caagcagttt
      961 tattaggaga aagtgttaac tctgccacac aagcgggagg aagtgtcgct tctgctgttt
     1021 tccagaacag cgcgtcgaca aatctagcag acctgacatt atcgaaatat caagttgaac
     1081 aactgtcaaa atatatcagt gaagcaatag aaaaattcgg ccaattgcag gaagtaattg
     1141 cagatctatt agcctcaatg tccaactctc aggctaatag aactgatgtt gcaaaagcaa
     1201 ttttgcaaca aactactgct tgatacaaat aaggagaatg ttatggaaat tcaaaacaca
     1261 aaaccaaccc agattttata tacagatata tccacaaaac aaactcaaag ttcttccgaa
     1321 acacaaaaat cacaaaatta tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa
     1381 aatcccgtat taacaaccac attaaatgat gatcaacttt taaagttatc agagcaggtt
     1441 cagcatgatt cagaaatcat tgctcgcctt actgacaaaa agatgaaaga tctttcagag
     1501 atgagtcaca cccttactcc agagaacact ctggatattt ccagtctttc ttctaatgct
     1561 gtttctttaa ttattagtgt agccgttcta ctttctgctc tccgcactgc agaaactaaa
     1621 ttgggctctc aattgtcatt gattgcgttc gatgctacaa aatcagctgc agagaacatt
     1681 gttcggcaag gcctggcagc cctatcatca agcattactg gagcagtcac acaagtaggt
     1741 ataacgggta tcggtgccaa aaaaacgcat tcagggatta gcgaccaaaa aggagcctta
     1801 agaaagaacc ttgccactgc tcaatctctt gaaaaagagc ttgcaggttc taaatt
//
LOCUS       DAZPFP010000350         1847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPFP010000350 DAZPFP010000000
VERSION     DAZPFP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.356"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..355)
                     /locus_tag="ACHUR4_004165"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHUR4_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHUR4_004166"
     CDS             complement(355..1275)
                     /locus_tag="ACHUR4_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1457483.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUR4_004167"
     CDS             1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUR4_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1457484.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          461 a          454 c          426 g          506 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtc
//
LOCUS       DAZPFP010000351         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPFP010000351 DAZPFP010000000
VERSION     DAZPFP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.357"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUR4_004168"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUR4_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR1457485.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACHUR4_004169"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACHUR4_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR1457486.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACHUR4_004170"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACHUR4_004170"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR1457487.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="ACHUR4_004171"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="ACHUR4_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1457488.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /gene="rem"
                     /locus_tag="ACHUR4_004172"
     CDS             1250..1501
                     /gene="rem"
                     /locus_tag="ACHUR4_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR1457489.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="ACHUR4_004173"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="ACHUR4_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          409 c          466 g          465 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAZPFP010000352         1834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPFP010000352 DAZPFP010000000
VERSION     DAZPFP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.358"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUR4_004174"
     CDS             complement(<1..182)
                     /locus_tag="ACHUR4_004174"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457490.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="ACHUR4_004175"
     CDS             complement(323..877)
                     /locus_tag="ACHUR4_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1457491.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="ACHUR4_004176"
     CDS             complement(874..1164)
                     /locus_tag="ACHUR4_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1457492.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="ACHUR4_004177"
     CDS             complement(1164..1763)
                     /locus_tag="ACHUR4_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1457493.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAZPFP010000353         1822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPFP010000353 DAZPFP010000000
VERSION     DAZPFP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.359"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHUR4_004178"
     CDS             complement(<1..362)
                     /locus_tag="ACHUR4_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1457494.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUR4_004179"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUR4_004179"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR1457495.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          544 a          472 c          376 g          430 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cg
//
LOCUS       DAZPFP010000354         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPFP010000354 DAZPFP010000000
VERSION     DAZPFP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.360"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHUR4_004180"
     CDS             173..1198
                     /locus_tag="ACHUR4_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1457496.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHUR4_004181"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHUR4_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZPFP010000355         1784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPFP010000355 DAZPFP010000000
VERSION     DAZPFP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.361"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHUR4_004182"
     CDS             7..657
                     /locus_tag="ACHUR4_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457497.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHUR4_004183"
     CDS             629..1063
                     /locus_tag="ACHUR4_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1457498.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          502 a          353 c          410 g          519 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agca
//
LOCUS       DAZPFP010000356         1780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPFP010000356 DAZPFP010000000
VERSION     DAZPFP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.362"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHUR4_004184"
     CDS             complement(<1..308)
                     /locus_tag="ACHUR4_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457499.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     T"
     gene            726..1724
                     /locus_tag="ACHUR4_004185"
                     /pseudo
     CDS             726..1724
                     /locus_tag="ACHUR4_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          470 a          361 c          356 g          593 t
ORIGIN      
        1 gtagtattat cggcaaaact attatattct tgaatatcta tagatggctc actattgtaa
       61 aaccatattt tctccatctg aagactatgt tcatttaccc tccagtgacc atgttgcagg
      121 aaatagatga tatgcatgcc aaaatcgtta tctatttgcg aatcattatc cagcttatca
      181 atttcaacta tctttgaacg atgtgtttta tcaaccttgc tatggtgttg atgattatta
      241 actttatctg aatgcttatc tttatggttt gaagggatgt acttaaatat attgtcaatt
      301 gcgttcataa ataacccgcg atatttttga cctgttagta aaaaataaat gatacatgtc
      361 tgaaagacat atttacgacg atataaaaca ataatactta tatgttcgct gattttaagc
      421 accgagacta tccggtcttt tactaaataa tggaaaacaa tccatccctg gaagacaatt
      481 gtaaaaaaaa acaacagaat aagaattaat cttaggataa atatttattt atcatggcct
      541 tttgaccgct aaaaaatggc actcggcaca gtaaaccccg ctatagttat gctatgcatt
      601 tgtctttaat gatatctcgt cgttatatta acaggctgaa attatcatta atgcacattt
      661 caatattagc agggatacct gaggagttta aagtgaacat atatatcggg tagcttttca
      721 aattaatccc tttgattata ggcttaattt gcatcgcgtt aggtggcttt gtgctggaaa
      781 gttcagggca acgtcacggg tcatgtgctg atttctctgg cggccatatg cctggcatta
      841 ttcactaccg catttattat catttcgcaa ctcacgcgcg gcgttaatac gttttacaat
      901 acattgttcc ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca
      961 ctgttagcag gtaatgatgt gatggcagac gagtttgttg ccggccatgt tatttttggc
     1021 gttggtatga ttgccgcatg tgtatcgacg gtggcagcgt cgtccggtca ctttctgctc
     1081 attcccaaaa atgcagcggg gagcaagagc gacggaacac cggtacaggc ttattcttca
     1141 ttaatcggta attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg
     1201 tctattacgc tgttacatag tgccgacata actccgcatt atgtcgcctt gggttaaccg
     1261 caatctgtgc ctgtctaatt ggcctagtgg ccacaatcgt ccatcaaaca cgtaatacgt
     1321 tctcatccaa agagcactgg ctgtggtgtt attgggttat ttttctcggc tcagtcacgg
     1381 tgctgcaggg gatatacgtc ttagtcagtt ccgatgcaag cgcccgactg gctcccggca
     1441 ttattctcat ttgcctcgga atgatctgtt acagcatatt ctcaaaagtc tggctactgg
     1501 cactggtctg gagacgtacc tgttcgttag ccaacagaat accgatgata ccggtattta
     1561 cctgtctgtt ttgcttgttt ttgtcatcat tcctcgcgga aatcgcacaa actgagctgg
     1621 actactttat tccatctcgg gttctggttg gattaggcgc agtatgtttt acattgttct
     1681 ctattgtttc aatattagag gccggttctg cgaaaaaaaa ttaaaattta cataatccca
     1741 gcgttattca tatcttaacg ctggtttttt acgaacttag
//
LOCUS       DAZPFP010000357         1776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPFP010000357 DAZPFP010000000
VERSION     DAZPFP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.363"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            171..1322
                     /locus_tag="ACHUR4_004186"
                     /pseudo
     CDS             171..1322
                     /locus_tag="ACHUR4_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1352..1776)
                     /locus_tag="ACHUR4_004187"
                     /pseudo
     CDS             complement(1352..1776)
                     /locus_tag="ACHUR4_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
BASE COUNT          446 a          445 c          469 g          416 t
ORIGIN      
        1 cattccagtt ataatattca ataatcaatg cgcgaggtgc tactaatggt attactgcgc
       61 ttacagatga tatatcagaa agcgcccgtt ttagttttaa tggagggtga tcttacccac
      121 caatagtgga cacaggacta agtgagtaaa ctctcaacca gaggtgactc atgacaaaac
      181 cagtatcaat cagcaagaag ccccgtaaac aacatacgcc tgaatttcgt aacgaagccc
      241 tgaaactcgc tgaacgcatc ggtgtggccg ccgcagcccg tgaactcagc ctgtatgaat
      301 ctcagcttta tgcctggcgc agtaaacagc agcaacaaat gagttcgtca gagcgcgaaa
      361 gcgaactggc cgctgaaaat gtccgcctta aacgacaact ggcggagcag gctgaggaac
      421 tgtccatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga agtatgtctt
      481 tatcgaaaat catcgggcag agttcagcat caaagcgatg tgtcgtgtac ttcgggttgc
      541 ccgcagcggc tggtatgtct ggctcaggcg tcgtcaccag atgagcctgc gccaacagtt
      601 tcggctcacc tgcgatgccg ctgtccataa ggcattcttt gaggcaaagc agcgatacgg
      661 tgctccccgc cttgctgacg aactgccgga gttcaatatt aaaaccattg ccgccagcct
      721 gcgtcgtcag gggctgcggg cgaaagccag ccggaagttc agcccggtca gctaccgtgc
      781 acatggcctg cccgtattgg agaatctgct ggagcaggac ttcagcgcca gtggcccgaa
      841 accagaagtg ggcgggtgac atcacgtact tgcgtaccga tgagggctgg ttgtatctcg
      901 cagtagtcat cgacctgtgg tcacgcgccg tcattggctg gtcgatgtca ccgcgaatga
      961 cagcacaact ggcctgtgat gcactgcaaa tggcgttgtg gcggagaaga cgcccggaaa
     1021 gcgtcattgt tcatacggac cgtggtggtc aatactgttc aggggattat caggcaatgc
     1081 tgaagcgaca caacctgcgt ggcagtatga gtgcgaaagg ctactgttat gacaatgcct
     1141 gtgtggaaag cttctttcat tcgctgaagg tggaatgtat ccacggggaa cgctttagca
     1201 gccgggaaat aatgcgggca acggtgttta attatatcga gtgtgattac aatcgctggc
     1261 gtcgtcacag tgcctgtggc ggtctcagcc cggaacaatt tgaaaaccat aatctcgctt
     1321 agggccgtgt ccacattacg tgggtaggat cagtatgggt cgtcttgtat ttgtcatact
     1381 gcccaatctc accaatcgaa aatgctcctt ttggcaaagg ttcaagcagg tctctgggaa
     1441 tggaggtcgc tggcgtcgga tataaaaaac cctcacggct tagcaaggct ggcgtcacta
     1501 tcggtgtaac aatatcaggc gtgcctgcgg aacggtcctt accatgctgc agtaaccgga
     1561 aataaacttt tgggcgtaag atgacattga cagtttcgac atcgtctttt tctcccttaa
     1621 aaaatttatt gacaatcgct tcgtcaagac gtcccgccgc tatcccatgc cagtgagtga
     1681 aattttgggc gtctttccgt tcaaaacagc cctttcctga ctcagcatct gcaagcgagt
     1741 tgcgccagta tgaggcaaac cttaaagcat tttcat
//
LOCUS       DAZPFP010000358         1751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPFP010000358 DAZPFP010000000
VERSION     DAZPFP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.364"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            456..1304
                     /locus_tag="ACHUR4_004188"
     CDS             456..1304
                     /locus_tag="ACHUR4_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1457500.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1321..1602
                     /locus_tag="ACHUR4_004189"
                     /pseudo
     CDS             1321..1602
                     /locus_tag="ACHUR4_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1638..>1751
                     /locus_tag="ACHUR4_004190"
     CDS             1638..>1751
                     /locus_tag="ACHUR4_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457501.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          500 a          399 c          411 g          441 t
ORIGIN      
        1 tcagcattat atagttctac tctggcgaga tacgttggca tattcatttc cttactggtt
       61 gtgtgagaac ttcagtaaag ataccaccaa agcccggaag tggtgaaata aaaccgggca
      121 caacacgaag gcgcatttcc ggtatccata aagagtcggt cttgtctgtg aaatttaaat
      181 ggtgggagtg cgcttccggt tgtaaataac gacattgctg tgtgaagtac cagttggcgg
      241 catcggttta attgctggct gatgtccgcc ctttttaaag tgaattttgt gatgcggtga
      301 atgcggctat gcgcacgcgg cacagttaaa agtcatgtta gtccttattg gtttgggtgg
      361 gaaagccaac tgtaattgtt aactggttaa cgtcacctgg aggcaccagg caccgcatca
      421 acaaagttca cttcggtgat gaaaggaaag agaaaatgtt gaatgtagct attgaaaacc
      481 agaacgggtg gaattatagt gcacctgcac ctcataaaac gggtgccggg attgctaccc
      541 cgacgatgac tactgcgcat aaccgcgctc aggcggtttt tttatgcgtc aagcacagcc
      601 acattcagat tatggtgggg cgtgcagggc agccgcaagg ctggccggtt tcggtagtca
      661 ccggttgtag caaccctgtg cgtctcacca cccatgagat tgctacctct ggtggtgagt
      721 catttaaact gactatcgag gctgccatca tggctactat ccttaccctt tctcaccctg
      781 acgcaaccat cgaaaatggt cgcgctgtca ctacgtctgt tgcggttgcg gagttcttcc
      841 ggaaaatgca caaaaacgtt attcagaaga tcgaaactct cgaatgctcg ccggagttta
      901 accggcttaa ttttaagccc gtcacctata ccgacgccaa aggcgaaaaa cgcccaatgt
      961 accaaatcac caaaaacggc ttcgttttcc tggtgatggg cttcactggc aaaaaagccg
     1021 ccgcattcaa ggaagcctac attgctgagt tcgaccgcat ggaggccgaa ctgcgccaga
     1081 ataacacccc acctgcagac aagatgattc cgggtgatgg tcgcactctg gttgttcact
     1141 tcgacaaatt cggcaatgtc gaattcaccg aaaccgttcc tgatggcgca ctggtctgta
     1201 ccctggagac tttccggttt tatctggaaa aacagggatg gactcttgtt aaccggggcg
     1261 caattaaaaa tatgactgtg gaacaattac tttctttaaa ataaaaatgg agctacggat
     1321 atgaacccta tgcttgatta ttgttttacc tctgcacctc agcagaccat aacacacacc
     1381 ggaatcaaaa atttgttatg gataatcaaa aaaaacctcc agtagaaagg tgatcccgat
     1441 ttaattccct gtgcttatgg cctgtgtgac agacaagtaa gccctgataa ggcttacact
     1501 gctgaactta tttacatgca cggaaccatg atgaggaaaa agaaacgcaa atattgtagt
     1561 gaaatctgcg ccgaaaaaga ccagatggca cacgaacttt aattaactga ctattcgaaa
     1621 ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga
     1681 acaccaatta tgaagccact gttgcaacta ctgataacat tgttcacgag gtttacctgg
     1741 aaggaaagca t
//
LOCUS       DAZPFP010000359         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPFP010000359 DAZPFP010000000
VERSION     DAZPFP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.365"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHUR4_004191"
     CDS             311..505
                     /locus_tag="ACHUR4_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457502.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHUR4_004192"
     CDS             866..994
                     /locus_tag="ACHUR4_004192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457503.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHUR4_004193"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHUR4_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          520 a          388 c          373 g          463 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttct
//
LOCUS       DAZPFP010000360         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPFP010000360 DAZPFP010000000
VERSION     DAZPFP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.366"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUR4_004194"
     CDS             complement(<1..353)
                     /locus_tag="ACHUR4_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1457504.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHUR4_004195"
     CDS             complement(350..940)
                     /locus_tag="ACHUR4_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1457505.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHUR4_004196"
     CDS             complement(927..1298)
                     /locus_tag="ACHUR4_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1457506.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUR4_004197"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUR4_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1457507.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZPFP010000361         1733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPFP010000361 DAZPFP010000000
VERSION     DAZPFP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.367"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHUR4_004198"
     CDS             <1..143
                     /locus_tag="ACHUR4_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1457508.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHUR4_004199"
     CDS             127..486
                     /locus_tag="ACHUR4_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457509.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHUR4_004200"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHUR4_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR1457510.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHUR4_004201"
     CDS             707..1141
                     /locus_tag="ACHUR4_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457511.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1733
                     /locus_tag="ACHUR4_004202"
     CDS             1063..>1733
                     /locus_tag="ACHUR4_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457512.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          569 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gga
//
LOCUS       DAZPFP010000362         1722 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPFP010000362 DAZPFP010000000
VERSION     DAZPFP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.368"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            214..1257
                     /locus_tag="ACHUR4_004203"
     CDS             214..1257
                     /locus_tag="ACHUR4_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1457513.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          508 a          367 c          361 g          486 t
ORIGIN      
        1 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
       61 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
      121 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
      181 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
      241 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
      301 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
      361 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
      421 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
      481 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
      541 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
      601 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
      661 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
      721 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
      781 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
      841 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
      901 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
      961 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
     1021 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
     1081 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
     1141 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
     1201 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
     1261 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
     1321 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
     1381 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
     1441 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
     1501 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
     1561 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
     1621 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
     1681 catccaactt ttttaatatg gacaatagtt gtccatacgg tg
//
LOCUS       DAZPFP010000363         1717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPFP010000363 DAZPFP010000000
VERSION     DAZPFP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.369"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHUR4_004204"
     CDS             complement(110..577)
                     /locus_tag="ACHUR4_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1457514.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHUR4_004205"
     CDS             complement(643..882)
                     /locus_tag="ACHUR4_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR1457515.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHUR4_004206"
     CDS             complement(1017..1622)
                     /locus_tag="ACHUR4_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1457516.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAZPFP010000364         1712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPFP010000364 DAZPFP010000000
VERSION     DAZPFP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.370"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            93..743
                     /gene="zinT"
                     /locus_tag="ACHUR4_004207"
     CDS             93..743
                     /gene="zinT"
                     /locus_tag="ACHUR4_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1457517.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          547 a          332 c          331 g          502 t
ORIGIN      
        1 catgctctca tttcctaatg agttgttgca ttttttcata tgttacaata taacattaca
       61 cataatgtac attaactctg gaggaaattg ttttggcgat tcgtcttcat aaactggctg
      121 ttgctttagg tgtctttatt gttagcgctc ctgccttttc gcatggtcat cattctcatg
      181 gcaaaccctt aacagaggtc gaacaaaaag ctgctaatgg tgtttttgat gataccaatg
      241 tacaaaaccg aacgctcagt gactgggatg gagtctggca atccgtttac cctttactgc
      301 aaagtggcaa acttgatccc gtctttcaga agaaagcgga tgcagataaa accaaaacat
      361 ttgctgaaat taaagattat tatcgcaaag gttatccgac agatatcgaa atgattggca
      421 ttgaggacgg cattgtcgaa ttccatagaa ataatgaaac aacatcctgt aaatatgatt
      481 acgatggata caaaatactc acctataaat caggcaagaa aggcgttcgt tacttattcg
      541 aatgtaaaga tcctgaaagc aaagccccta aatatataca atttagcgat catataattg
      601 caccacgaaa atcatctcat tttcacattt ttatgggtaa tgattcccag caatctttat
      661 tgaatgaaat ggaaaactgg ccaacgtatt atccctatca gttgagtagc gaagaagtgg
      721 tcgaggagat gatgtctcat tgattttttt gcgctgactt cagttattta acagaacagt
      781 gcctggtagt aacaacaata atttaccaag acaaataagt aagacaaact cagacacgct
      841 catacttgag tcgtgtcttt tttattatgt ctgtagaaaa aaactgagaa aaaggctaac
      901 aataaataca caactcagcc atacagactg gcagcgcaaa tgtttccagg agcaagtcat
      961 ctgtcatggt aaagctaacc agaaatatag gttaattttg gtgtttaaac cttttttact
     1021 tgttagtatg atacaaaggc tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta
     1081 tggactttgt cccgcggctt taatctgacg gccgcgttcc ttttttggct caattattga
     1141 ccccttctct acgcaacttc agttcccgcc agcaactttg cggcagtttc ataggatcaa
     1201 tgtctaaaag aataatggtg accgataaga aaacgactga ataactgtag attttcgctc
     1261 gaaaccttcc tgtcagctcc atagcgaatc aagcgctgaa tgtcacagta tcgaacagaa
     1321 aacagtgacg atctaaccct tcaagaatat tctacgattg ttctgtttag gaaaagcaag
     1381 gcgggaagtc gggagataag tcattgataa aatggcggag agagggggat ttgaaccccc
     1441 ggtagagttg cccctactcc ggttttcgag accgatgata cagagtcgaa aaatcaaaga
     1501 gttaaaaatt tttcttggaa aaaatgtctt aaaatcgtac acgaaaaatc aatgaattac
     1561 ataacattaa agaagaatat tccaagcctt cttgagtcca ccacctgttt tcaggtgtca
     1621 ttcagacgtg atttgcccca gattgggaaa gcacgctttt ttctcagctt attccgtttt
     1681 gcctgtgtgt tgaataattc cactgcagtt at
//
LOCUS       DAZPFP010000365         1702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPFP010000365 DAZPFP010000000
VERSION     DAZPFP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.371"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHUR4_004208"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHUR4_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1457518.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHUR4_004209"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHUR4_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHUR4_004210"
     CDS             1152..1493
                     /locus_tag="ACHUR4_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1457519.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT          410 a          396 c          442 g          454 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tc
//
LOCUS       DAZPFP010000366         1688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPFP010000366 DAZPFP010000000
VERSION     DAZPFP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.372"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            36..437
                     /gene="spa15"
                     /locus_tag="ACHUR4_004211"
                     /gene_synonym="spaK"
     CDS             36..437
                     /gene="spa15"
                     /locus_tag="ACHUR4_004211"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR1457520.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            441..>1688
                     /gene="spaL"
                     /locus_tag="ACHUR4_004212"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             441..>1688
                     /gene="spaL"
                     /locus_tag="ACHUR4_004212"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR1457521.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGF"
BASE COUNT          496 a          257 c          377 g          558 t
ORIGIN      
        1 ttaatgtatt aaagactatt tagtgaggtt taaatatgag taacattaat ttagttcaat
       61 tagttagaga tagtcttttc acgattggtt gtcctccatc aattattaca gatttagaca
      121 gccattcagc gattaccatt tctttggata gtatgcctgc tattaatata gctcttgtca
      181 atgagcaggt tatgttatgg gctaattttg atgcaccaag tgatgtaaaa cttcagtcat
      241 ctgcttataa catattaaat ttaatgttaa tgaactttag ttatagcatt aatgagctgg
      301 tagagcttca tcgttctgat gagtacttac agttaagagt tgttattaaa gatgactacg
      361 ttcacgatgg gattgttttt gcagagattc tgcatgagtt ttatcagagg atggaaatct
      421 taaatggggt cttataatca atgagctata caaaattgct cactcaatta tcttttccta
      481 atagaatctc ggggccaatc ttggaaacaa gtcttagcga tgtttcgatt ggtgagattt
      541 gtaacattca ggctggaatt gaaagtaatg aaattgttgc aagagctcag gttgtaggat
      601 ttcatgatga aaaaacaata ttaagcttga ttggaaattc tcgtggactt tcacggcaaa
      661 cgttgattaa gcccactgcc cagtttcttc atacgcaagt cggccgtgga ttattgggag
      721 cagtagtcaa tcctttaggg gaggttactg ataagtttgc tgttacagat aacagtgaaa
      781 ttctttatcg acctgtagat aatgctcctc cgctatatag tgaaagggct gcaattgaga
      841 agcctttttt aacaggtatt aaggttattg attctttact cacgtgtggt gaaggacagc
      901 gaatggggat ttttgcgtca gctggttgtg gcaaaacttt tctcatgaat atgctcattg
      961 aacatagtgg tgctgatata tatgttattg ggttaattgg tgagcgaggt cgagaggtta
     1021 ctgaaacggt tgattatttg aaaaactctg agaaaaaaag caggtgtgtt ttagtatatg
     1081 caacttcgga ttactcttcg gttgatcgtt gtaatgctgc atatatagcc actgctatag
     1141 ccgaattttt taggactgaa ggacataaag tagcgctttt tattgattca ttaacaaggt
     1201 atgccagagc attacgtgat gtggccttag ccgctggaga atcacctgcc agaagaggct
     1261 atccggtttc ggtttttgat agcttaccca gacttcttga aaggccagga aagttaaagg
     1321 caggtggctc tattactgca ttttacactg ttcttttgga ggatgatgat tttgctgatc
     1381 cattagctga agaggtaaga tccattttag atggacatat atatttgagc agaaatctag
     1441 cccaaaaagg acaatttcct gcaattgatt ccttaaaaag tataagcagg gtatttacac
     1501 aggttgttga tgaaaaacat cgtattatgg ccgctgcatt tcgggagcta ctttctgaaa
     1561 tagaagagct aaggacaatt attgactttg gtgaatacaa accgggggag aatgcctctc
     1621 aggataaaat ctacaacaaa atatctgttg ttgagagttt tctgaagcaa gattatcgac
     1681 tcggtttt
//
LOCUS       DAZPFP010000367         1645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPFP010000367 DAZPFP010000000
VERSION     DAZPFP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.373"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(72..148)
                     /locus_tag="ACHUR4_004213"
     tRNA            complement(72..148)
                     /locus_tag="ACHUR4_004213"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACHUR4_004214"
     tRNA            complement(150..225)
                     /locus_tag="ACHUR4_004214"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACHUR4_004215"
     CDS             complement(402..1091)
                     /locus_tag="ACHUR4_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1457522.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACHUR4_004216"
     CDS             complement(1088..1447)
                     /locus_tag="ACHUR4_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1457523.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..1635)
                     /locus_tag="ACHUR4_004217"
     CDS             complement(1447..1635)
                     /locus_tag="ACHUR4_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457524.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          394 a          462 c          352 g          437 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttcccga agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct
     1621 gaaatagaat ggcatcagaa ggagc
//
LOCUS       DAZPFP010000368         1623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPFP010000368 DAZPFP010000000
VERSION     DAZPFP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.374"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            36..1241
                     /gene="tet(B)"
                     /locus_tag="ACHUR4_004218"
     CDS             36..1241
                     /gene="tet(B)"
                     /locus_tag="ACHUR4_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1457525.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1354..>1623)
                     /locus_tag="ACHUR4_004219"
     CDS             complement(1354..>1623)
                     /locus_tag="ACHUR4_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1457526.1"
                     /translation="PIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDD
                     DVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          401 a          303 c          365 g          554 t
ORIGIN      
        1 tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat
       61 tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt
      121 tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac
      181 tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg
      241 gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg
      301 ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag
      361 ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga
      421 agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg
      481 gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata
      541 ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata
      601 cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa
      661 cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa
      721 cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt
      781 cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag
      841 ccactaaatg gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat
      901 ttgccttttt agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg
      961 ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc
     1021 atcagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg
     1081 gcccattact gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt
     1141 ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa
     1201 cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga
     1261 tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa
     1321 agaagattta gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa
     1381 gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc
     1441 atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat catcatcata
     1501 agctttaagt tggttctctt ggatcaattt gctgacaatg gcgtttacct taccagtaat
     1561 gtattcaagg ctaatttttt caagttcatt ccaaccaatg ataggcatca cttcttggat
     1621 agg
//
LOCUS       DAZPFP010000369         1603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPFP010000369 DAZPFP010000000
VERSION     DAZPFP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.375"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHUR4_004220"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHUR4_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHUR4_004221"
     CDS             535..717
                     /locus_tag="ACHUR4_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1457527.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHUR4_004222"
     CDS             complement(818..1147)
                     /locus_tag="ACHUR4_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1457528.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHUR4_004223"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHUR4_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457529.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZPFP010000370         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPFP010000370 DAZPFP010000000
VERSION     DAZPFP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.376"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUR4_004224"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUR4_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR1457530.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHUR4_004225"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHUR4_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR1457531.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZPFP010000371         1543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPFP010000371 DAZPFP010000000
VERSION     DAZPFP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.377"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(184..303)
                     /locus_tag="ACHUR4_004226"
                     /pseudo
     CDS             complement(184..303)
                     /locus_tag="ACHUR4_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            547..919
                     /locus_tag="ACHUR4_004227"
                     /pseudo
     CDS             547..919
                     /locus_tag="ACHUR4_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1481..>1543)
                     /locus_tag="ACHUR4_004228"
     CDS             complement(1481..>1543)
                     /locus_tag="ACHUR4_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457532.1"
                     /translation="NSASIEYIGFLTSAGIKYIF"
BASE COUNT          453 a          347 c          345 g          398 t
ORIGIN      
        1 tgcaagcttc tttgtatact atgatttaaa aaaacggaac aaaaatgcaa ttatattctg
       61 gcattgttta atgcggtttg tcttttttat tcattatttc aatgaaagaa tttcaactac
      121 aaagttaatt gatcctacct acataatata gacacagtac ctaaagtacc gtggagatca
      181 tccggggaag ccccgggtct gataccagtg caggtagacc gctatcttgt tccggtaatg
      241 ttctgccgtt ctttacggca tcacctctag cagaaacatc gcgtaactgc gccaagtatg
      301 atgaacttct cttaccgctg cgatctggtt caacaatatg cagatggtac acaacctctt
      361 taaccagccc atccggatta ttcagagccg cttccgcaat ccggaataaa agtgctgttt
      421 taccgtgaac cctctgaaaa tcagcgagca gtgttttaac cagccgacgt tcagagcgta
      481 ctgacagacg gtggacaatt tgaataaaca gttcaatcag accgtagatg ctctcccgtt
      541 ggcgacatgc tgggagcggt ggaacaccgc ttcggcaacg agcttccggc gtctccagtg
      601 aagtgactga cggataatgg ttcatgctac cgagctaatg aaacacgcca gttcacccgg
      661 atgttgggac tttgttgaac cgaagaacac ggcggtgcgg agtccggaga gtaacagaat
      721 agcaaagagc ttcgtgaaaa caataaagtg tgactacatc agtatcatgc ccaaacctga
      781 cgggttaacg gcagcaaaga accttgcaga ggcattcgag cattataacg aatggcatcc
      841 gcatagtgcg ctggattatc gctcgccacg ggaatatctg cggcagcggg ccaatgataa
      901 caggtgtctg gaaatatagg ggcaaagcca cccacgaacg atccagcata taatcaagaa
      961 attagatttc atttaacata agatagatta tgtacaccaa gaggcgtttc cttaatttct
     1021 gaatttacag gctcttttgc tctctttgct atgctgtgga tttggaaaga tagagtgctg
     1081 gctgattatt taggtatttt catcagcatg tccctgatag cactgttcca tcagcgcttt
     1141 caggcgataa tagtagacct tgggtagcct ctgcccatgt gtaacttgat accccgagaa
     1201 cagccacgat gatctaagcg gttcttattt ctccggtctc agggtcggta atgccaacag
     1261 taagtccaca gtaatcaacg aagagttttt tgaaccgccc cggaaatcct ggagactaaa
     1321 cgccctgaga aagaggtaaa cagaatgact aaaaatacac gtttttcccc cgaagtccgt
     1381 caacgggcag ttcgtatggt tctggaaagt cagggcgaat atgactcaca atgggcggca
     1441 atttttagcc atgaaaacgt aatcaactcg gtgaaccaat tcaaaaaata tactttatac
     1501 ctgcggaagt gagaaaacca atatattcaa tacttgcact att
//
LOCUS       DAZPFP010000372         1532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPFP010000372 DAZPFP010000000
VERSION     DAZPFP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.378"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..291
                     /locus_tag="ACHUR4_004229"
                     /pseudo
     CDS             1..291
                     /locus_tag="ACHUR4_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="ACHUR4_004230"
                     /pseudo
     CDS             293..659
                     /locus_tag="ACHUR4_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            662..1060
                     /locus_tag="ACHUR4_004231"
                     /pseudo
     CDS             662..1060
                     /locus_tag="ACHUR4_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          379 a          380 c          461 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAZPFP010000373         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPFP010000373 DAZPFP010000000
VERSION     DAZPFP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.379"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            71..352
                     /locus_tag="ACHUR4_004232"
     CDS             71..352
                     /locus_tag="ACHUR4_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1457533.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="ACHUR4_004233"
                     /pseudo
     CDS             318..1395
                     /locus_tag="ACHUR4_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          525 a          299 c          341 g          360 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaat
//
LOCUS       DAZPFP010000374         1500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPFP010000374 DAZPFP010000000
VERSION     DAZPFP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.380"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHUR4_004234"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHUR4_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUR4_004235"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUR4_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR1457534.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHUR4_004236"
     CDS             complement(1164..1349)
                     /locus_tag="ACHUR4_004236"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1457535.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..>1500)
                     /locus_tag="ACHUR4_004237"
     CDS             complement(1374..>1500)
                     /locus_tag="ACHUR4_004237"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR1457536.1"
                     /translation="GVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          420 a          378 c          262 g          440 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
//
LOCUS       DAZPFP010000375         1490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPFP010000375 DAZPFP010000000
VERSION     DAZPFP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.381"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHUR4_004238"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHUR4_004238"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1457537.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          402 a          309 c          405 g          374 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata
//
LOCUS       DAZPFP010000376         1464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPFP010000376 DAZPFP010000000
VERSION     DAZPFP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.382"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            182..310
                     /locus_tag="ACHUR4_004239"
                     /pseudo
     CDS             182..310
                     /locus_tag="ACHUR4_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            500..904
                     /locus_tag="ACHUR4_004240"
     CDS             500..904
                     /locus_tag="ACHUR4_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457538.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            967..1464
                     /locus_tag="ACHUR4_004241"
                     /pseudo
     CDS             967..1464
                     /locus_tag="ACHUR4_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          405 a          358 c          392 g          309 t
ORIGIN      
        1 atgtattgag ctgtcatact gagaaacaaa ccttagggca gcacaattaa agccagaaac
       61 aactatgcaa gaaagttact aaataagtgc tcggaacagt aaaacactca ggggggactc
      121 agtccggatt atgtaatcag ataaattgcc aaacatcaca aatcaccagc cggactgagt
      181 gatgccgagc attgcaccaa taccccgtga tgaacgatgc ctgatgcaga aagctcccta
      241 taaaacaaag aacaaaaact acgaccggcg attcaccgtc atactgatgc tgcatcgtgg
      301 tgacactgtc tgtcactttc atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc
      361 cggcgggatt tgattgtccg gcgtctagct tccacttccc tgatggaact gaaagttgtt
      421 atggccggtc agcttgccct gtccgcagga catgaggtgc tgtgacggac agcaccagta
      481 acggaaagtg acaatgaaaa tgaatactgc gctgatagtt gcactaatgt gtatgtggta
      541 tgcggtaccc gctgctgcaa aagagacttt actggctatg ccacgtaatt caacagaaca
      601 ttgctatgcg gagattaatg tgcacggacc atatggggtg tatttccgag tggtcccgca
      661 tccccccggg ggaaagagct gggtcgaatg taacagtgac tactattact ctgataaacc
      721 accgggagta cagatactcg gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac
      781 cacctcgaca ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc
      841 acgtgggatg ataattcaca ggatacggaa acgtcctgta gtggctgtaa gcgatgagat
      901 gtgatcttac ccagcaatag tagacacgcg gctaagtgag taaactctca gtcagaggtg
      961 actcacatga caaaaacagt atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa
     1021 ttttgcagtg aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc agcccgtgaa
     1081 ctcagcctgt atgaatctca gctctatgcg tggcgcagca aactgcagca acagatgacc
     1141 tcgtctgagc gtgaaagtga actgcccgcc tgaagcggca actggcggag caggcagagg
     1201 aactggctat cctccaaaag gccgcgacat actttgcgca gcggctggta tacgtggtgt
     1261 cagcgtcgga caggaataag ccctcgtcag cagttccgtc agcactgcga cagcgttgtc
     1321 ctcgcggcgg cctttacccg gtcaaaacag cgttacggtg caccacgcct gacggatgaa
     1381 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
     1441 gggctgaggg caaaggcctc ccgg
//
LOCUS       DAZPFP010000377         1448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPFP010000377 DAZPFP010000000
VERSION     DAZPFP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.383"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            40..1029
                     /gene="ldhA"
                     /locus_tag="ACHUR4_004242"
     CDS             40..1029
                     /gene="ldhA"
                     /locus_tag="ACHUR4_004242"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1457539.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1140..>1448
                     /gene="hslJ"
                     /locus_tag="ACHUR4_004243"
     CDS             1140..>1448
                     /gene="hslJ"
                     /locus_tag="ACHUR4_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1457540.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT          391 a          341 c          365 g          351 t
ORIGIN      
        1 gtagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc
       61 acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg
      121 gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg
      181 gtatgtattt tcgtaaacga tgacggcagc cacccggtgc tggaagagct gaaaaagcac
      241 ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgatgcggca
      301 aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa
      361 cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc
      421 cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca
      481 ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt
      541 atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg cgctggaact cggtgtggag
      601 tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg
      661 acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg
      721 atgatcgtca ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg
      781 aaaaatcaga aaattggttc gttgggtatg gacgtctatg agaacgaacg cgatctgttc
      841 tttgaagata aatccaacga cgtgatccag gatgacgtat tccgtcgcct gtctgcctgc
      901 cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt
      961 tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg cccgaacgaa
     1021 ctggtttaat cttgcagctc ccctgcaatc caggggagct gattcagata atccccaatg
     1081 acctttcatt ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa
     1141 tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg
     1201 acaaaattgc tgttacgcca gaacagctac agcatcatcg ctttgtgctg gaaagcgtaa
     1261 acggtaagcc cgtgaccagc gataaaaatc cgccagaaat cagctttggt gaaaaaatga
     1321 tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac
     1381 tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac
     1441 tcgataac
//
LOCUS       DAZPFP010000378         1443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPFP010000378 DAZPFP010000000
VERSION     DAZPFP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.384"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            82..1275
                     /locus_tag="ACHUR4_004244"
     CDS             82..1275
                     /locus_tag="ACHUR4_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR1457541.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="ACHUR4_004245"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="ACHUR4_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAZPFP010000379         1434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPFP010000379 DAZPFP010000000
VERSION     DAZPFP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.385"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            431..600
                     /locus_tag="ACHUR4_004246"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHUR4_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="ACHUR4_004247"
     CDS             746..>1434
                     /locus_tag="ACHUR4_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1457542.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAZPFP010000380         1416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPFP010000380 DAZPFP010000000
VERSION     DAZPFP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.386"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHUR4_004248"
     CDS             <1..294
                     /locus_tag="ACHUR4_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1457543.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHUR4_004249"
     CDS             300..1397
                     /locus_tag="ACHUR4_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1457544.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          439 a          320 c          348 g          309 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttggga
//
LOCUS       DAZPFP010000381         1408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPFP010000381 DAZPFP010000000
VERSION     DAZPFP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.387"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            28..432
                     /gene="stpA"
                     /locus_tag="ACHUR4_004250"
     CDS             28..432
                     /gene="stpA"
                     /locus_tag="ACHUR4_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1457545.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHUR4_004251"
     CDS             complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHUR4_004251"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1457546.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1013..1312)
                     /locus_tag="ACHUR4_004252"
     CDS             complement(1013..1312)
                     /locus_tag="ACHUR4_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1457547.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          381 a          331 c          335 g          361 t
ORIGIN      
        1 ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt
       61 cgcaccctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa
      121 aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg
      181 gctgagcgtc aggaaaaaat tagcacctgg ctggagctga tgaaagctga cggaattaac
      241 ccggaagagt tattgggtaa tagctctgct gctgcaccac gtgctggtaa aaaacgccag
      301 ccgcgtccgg cgaaatataa attcactgat gttaacggtg aaactaaaac ctggaccggt
      361 cagggccgta caccgaagcc aattgctcag gcgctggcag aaggtaaatc tctcgacgat
      421 ttcctgatct aattactgag gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc
      481 aagctcgttg gttccacggc atcttatcta acaaccttgc cattccacaa aaaccgctga
      541 ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa taagaagaaa ccgctattta
      601 cggtataacc cagtacaacg ccgtttaata ttaaaccacc cgcagcgatc tgcacctggc
      661 gcattaacgg caagggttga gatttattta ccgctactgg caatccagct cttttccagc
      721 catcaatccc atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct gctaatttat
      781 cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgcgacgta
      841 atttaggtgg aagacctgac tgttccagca cggataatgg agccagatct gcttcaggaa
      901 tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg ccgcgtgcga
      961 ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac
     1021 aatagacatt tttcagggtg gcgataatgg catttaccgc ctcattttta atggaatata
     1081 gaatgcgttg ggcatcccgt tggctgttga taagcccttc gtcccgcata cgagcgagat
     1141 gctgtgatgt cgcagaggcg ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc
     1201 cgggggaacc gctaagcatg cacagaatca gcaaccgttt agggtggctc attgctttca
     1261 ataaggccgc tgcctgttcg gtactggcct gtaattgcgc gagttcagtc atatttattt
     1321 aagtattttc taaattaagt aaactctaaa ctaaaaatgc aacatatacc agcctcagca
     1381 gcgtaaatga gagtaaaagc gtaagctg
//
LOCUS       DAZPFP010000382         1356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPFP010000382 DAZPFP010000000
VERSION     DAZPFP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.390"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            262..765
                     /locus_tag="ACHUR4_004253"
     CDS             262..765
                     /locus_tag="ACHUR4_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1457548.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(845..1096)
                     /gene="yecJ"
                     /locus_tag="ACHUR4_004254"
     CDS             complement(845..1096)
                     /gene="yecJ"
                     /locus_tag="ACHUR4_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1457549.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1211..1297)
                     /gene="azuC"
                     /locus_tag="ACHUR4_004255"
     CDS             complement(1211..1297)
                     /gene="azuC"
                     /locus_tag="ACHUR4_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1457550.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          379 a          306 c          290 g          381 t
ORIGIN      
        1 atcaacaaga tacaaaaagc actatcatta aaattcattg catttacatt gatttcacca
       61 atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttataac cgttcagacg
      121 ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg gaccggcaga
      181 aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac ctctccactt
      241 ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact caactctcaa
      301 atgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg gtgttctgaa
      361 cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa tgtgacccaa
      421 atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt taaagccatt
      481 gatgttccag gtgaaaaact gaactctctg gaagaactgt tccaaaaaac gatggaagaa
      541 tacgagcaac gttcaagcac tctggcgcag ttagccgatg aagcgaaaga actgaatgat
      601 gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga tggcctgttg
      661 ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag
      721 accgaccaac atgttctgaa tgtcgtgtca caccagctgc attgatcata catcggcgct
      781 aatacattgc gccgatgaag tttttgagaa accgctgcct catctgtttg aagcagcggt
      841 ttttttaatg ggattcaccc tgtggggtaa acttgagttc aatcagcgcg atggcttttt
      901 ggattgcccg catggtgacc gggtctgcgg cagcgggatg atgagtaaag tcgatattct
      961 tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg agaacatcca
     1021 gaatttgttt gataaccaac tggcaagcta ccacatcaga aaccagttcc tgatcggcat
     1081 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     1141 tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc
     1201 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     1261 tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta
     1321 tacaggtgtt attgtctttg cgcttataat atgaca
//
LOCUS       DAZPFP010000383         1334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPFP010000383 DAZPFP010000000
VERSION     DAZPFP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.391"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(3..485)
                     /locus_tag="ACHUR4_004256"
     CDS             complement(3..485)
                     /locus_tag="ACHUR4_004256"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1457551.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHUR4_004257"
     CDS             complement(476..778)
                     /locus_tag="ACHUR4_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1457552.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACHUR4_004258"
     CDS             1050..1322
                     /locus_tag="ACHUR4_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457553.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          334 c          284 g          324 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccg
//
LOCUS       DAZPFP010000384         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPFP010000384 DAZPFP010000000
VERSION     DAZPFP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.392"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHUR4_004259"
     CDS             complement(41..100)
                     /locus_tag="ACHUR4_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1457554.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHUR4_004260"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHUR4_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZPFP010000385         1329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPFP010000385 DAZPFP010000000
VERSION     DAZPFP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.393"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            147..1329
                     /locus_tag="ACHUR4_004261"
                     /pseudo
     CDS             147..1329
                     /locus_tag="ACHUR4_004261"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          373 a          239 c          335 g          382 t
ORIGIN      
        1 gaatattttt atgccatgca tattccatgt tttatcgact gtcattaatt ttgataatgg
       61 ccttacttgc gggtcagtta agtgcccagg agtggtcatt tgattcttca cagttggaag
      121 gaaacgttag tgcggacact gtggcaatgt ttaatcaggg agagcaactc cccggaaatt
      181 atcgtgtgga aatttatctt aacggtgaaa aagttgatgt tggggagttt ccctttcatc
      241 gtccggaatc ccctgaagaa aaagagcttg ttccatgttt aaccgttgat gaccttatcc
      301 actatggcat taaaattgat aaaagcagca gtgatactga caataaaaag aatcagtgtt
      361 ttaaatggaa ctctattgaa ggtttgaaag taaattatga ttttgatagc cagcgcgtac
      421 agataaccgt tccacaactg tatctgcaag ataaaaaaag tagtttagca ccagtgtcgt
      481 tatggaatga gggtgtagct gcattccgca tggtgtatca gacaaatatt gatatttcca
      541 aacaaaatga taatcaatct accacgcgta attctcgtta tggcaggttc actccagggt
      601 ttaatcttgg tgcctggagg tttcgtagtt cagtgacatg gagcaaggaa cttggccagt
      661 cagaaagatg gcaaaggggt tacatgtggt ttgagcgagg cataaatgcg attaaaagcc
      721 gattaacgct tggagaatct tatacgtcgt ctgaggtgtt tgacagcata ccttttcgcg
      781 gcgggatgtt agcgaccgac gatgcgatga ctccgccgga ggacagctac tacacacctg
      841 tggtgcatgg aatagcacaa agtgaggcgc aggttattat taaacaaaat gggcaaatta
      901 tttttacccg ttctgtgccg ccagggcctt ttgcgctgga taatttacca acgctggccg
      961 ttggcgggga gcttgatgtt acagtgagag agagtaatgg cgaggagcag tatttttccg
     1021 tgccatttca aacgccggcc atcgctctgc atgaaggata tttcaaatat agtgtgatgg
     1081 gggggaatat aaagaagaag gtataaaaag ctcccctgtc tccgtgggcg aaatcacggc
     1141 gatgtacggg cttccttatg gcgtcacggt gtatggaggg attcaaaacg ccactcattt
     1201 taatgcaata tctacgggta ttggcatttc tctggggctg ttagggagtt tatcgacaga
     1261 tattacgcgt tcgattgcga atttatatta tgggaataaa tatcgtattc gttatagtaa
     1321 atccattag
//
LOCUS       DAZPFP010000386         1322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPFP010000386 DAZPFP010000000
VERSION     DAZPFP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.394"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUR4_004262"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUR4_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUR4_004263"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUR4_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1457555.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          358 a          307 c          283 g          374 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 at
//
LOCUS       DAZPFP010000387         1301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPFP010000387 DAZPFP010000000
VERSION     DAZPFP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.395"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..479
                     /locus_tag="ACHUR4_004264"
     CDS             <1..479
                     /locus_tag="ACHUR4_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1457556.1"
                     /translation="LRDAHAMEKQAESMLESMASRIDNYPELRARIEQHLSETKNQIV
                     QLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYVFEQFEITCYT
                     SLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGVEAKK"
     gene            complement(539..1177)
                     /gene="ompW"
                     /locus_tag="ACHUR4_004265"
     CDS             complement(539..1177)
                     /gene="ompW"
                     /locus_tag="ACHUR4_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1457557.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          378 a          310 c          274 g          339 t
ORIGIN      
        1 ggttacgtga cgcccacgca atggaaaagc aagccgaatc tatgcttgaa tccatggcca
       61 gccgtataga taattatcct gaactacgcg ctcgtattga acaacatctt agtgaaacca
      121 aaaaccagat tgttcaactg gaaactattc ttgatcgtaa tgacatttca cgttcagtca
      181 ttaaagattc catgagtaaa atggctgcgc ttgggcagtc aatcggtggt atattccctt
      241 ctgatgaaat agttaaaggc tctattggcg gatatgtctt cgagcaattt gaaatcacct
      301 gttacacctc actattagca gcagcaaaaa atgccggtga tacagtctca attccgatca
      361 tcgaagcgat tttaaatgag gaaaagcaaa tggccgactg gctgattcag catattccgc
      421 aaacaactga gaaattttta attcgttctg aaactgatgg cgtagaagcg aagaaataat
      481 accctttata ccatgtcctt attgaccccg tatattacgg ggtcgttttt gtgcggaatt
      541 aaaaacgata tcctgctgag aacataaaca cccacggatc gaggcgtacg ctatcgtgtt
      601 gctgtgcacc gcctagctta taattggcgg tggtatcgat atccatgtac cacactgaca
      661 tgttaaccaa ccagtcacgg ttaatcagat aatcaacccc cacctgcccg gcagctcccc
      721 aggaatcttt cagactgaga tcggaaagcc ccgcctcttt gccatgatcg ttaaatccat
      781 tatcaaagaa ggtggtgtag ttaataccgg ccccaacgta aggacggaat ttgctgctgg
      841 catcaccaaa ataccactgc gccatcagtg ttggcggcag atggtgaacg gttgcaatat
      901 cgccggtcgc ccgggtgccg attttatggc ggaacggcgt cgctgccagt aattccacac
      961 caatgttgtc ggtcgccata taagtaaacg tcaagcccag ttgagtgtta ttggtcacgc
     1021 tgaatccacc cagacttcct aacgtaccac cagcaccttc tgttggacgt acggttgcag
     1081 aacctgcacg cataaaaaat tcgcctgctt catgcgcaaa ggcactgcca gaaagaagag
     1141 ttgttactgc caaagccgcc actgttaact ttttcatatc cgctccgtcg ttatggttat
     1201 aaaaacgtga cgaatatacc tacattggag taacaaatga tcctacatag atcacattac
     1261 atatgataat tcaccattta ttgatctgga ttaatttaga a
//
LOCUS       DAZPFP010000388         1299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPFP010000388 DAZPFP010000000
VERSION     DAZPFP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.396"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUR4_004266"
     CDS             complement(<1..143)
                     /locus_tag="ACHUR4_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR1457558.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..>1299)
                     /gene="ydeO"
                     /locus_tag="ACHUR4_004267"
     CDS             complement(545..>1299)
                     /gene="ydeO"
                     /locus_tag="ACHUR4_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1457559.1"
                     /translation="VCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERH
                     FKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHN
                     FSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYIS
                     ESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRK
                     HFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT          412 a          241 c          246 g          400 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac catcaggatc tcgccgtcgg agaaggcgta atctttatct aaaatgttag
     1261 cgttgaaagc atgatgaata aatataacag aacaaacga
//
LOCUS       DAZPFP010000389         1274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPFP010000389 DAZPFP010000000
VERSION     DAZPFP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.397"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(308..>1274)
                     /locus_tag="ACHUR4_004268"
     CDS             complement(308..>1274)
                     /locus_tag="ACHUR4_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1457560.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          382 a          275 c          274 g          343 t
ORIGIN      
        1 atgttttaga tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt gttgtattag
       61 cgactgaaat ctgcctttta aaaaggagga gggtactgag acaaagagtc attgcttctc
      121 cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac ttgttgaccg
      181 agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg attatcctgt
      241 ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt ttactgctgg
      301 catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata cctaatataa
      361 ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat atcacttccg
      421 tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga cccattcctt
      481 ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac agcggaatac
      541 cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga gcggcaatcc
      601 acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac aaaagatatg
      661 gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg ggattaatac
      721 ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct ttagccgctg
      781 gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt tttgctggag
      841 cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac cccagagaat
      901 ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac aacaaggtga
      961 ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt aatggtgaaa
     1021 caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca
     1081 tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca gccaggagat
     1141 agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc accttttgtc
     1201 ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg acggcgagaa
     1261 acacaaacat ctct
//
LOCUS       DAZPFP010000390         1245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPFP010000390 DAZPFP010000000
VERSION     DAZPFP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.398"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            112..>1245
                     /gene="pdeF"
                     /locus_tag="ACHUR4_004269"
     CDS             112..>1245
                     /gene="pdeF"
                     /locus_tag="ACHUR4_004269"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1457561.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          280 a          279 c          280 g          406 t
ORIGIN      
        1 acacttagaa tcaatgctct ctatagtgat gaataatcat cattcgaagt caggtgggat
       61 gcctgtctga atacacctcc ttcaggatgt gggggattct gctgagcatc tatgaaactg
      121 aatgcaactt atataaaaat acgtgataaa tggtgggggc ttccgctgtt cctgccttct
      181 ttaatcttgc ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaggtt
      241 tttctctttt atctgccttt ggcactgatg atcagcatga tgatgttttt cagctgggcg
      301 gcattgccag ggatcacctt agggattttt gtccgcaaat atgcagagct gggtttttac
      361 gaaacgctct cattaacggc taattttatt atcattatca ttctctgttg gggcggttac
      421 agggtcttta ctccccggcg taacaacgtt tcacatggtg atacccgttt aatttcccag
      481 cgtatattct ggcagatagt gtttcctgca acgctgtttc tgatactttt ccagtttgct
      541 gcatttgtag gattactggc gagcagagaa aatctggtcg gtgtcatgcc ttttaacctc
      601 gggactttaa tcaattatca ggccttactg gtgggtaatc tgatcggtgt cccgctgtgc
      661 tacttcatca ttcgggtagt gcgaaatcca ttttatttac gtagctatta ttcgcaatta
      721 aaacagcagg ttgatgccaa agtcaccaaa aaagagttcg cgctctggct actggcatta
      781 ggtgctttat tgttgctgtt atgcatgccg ttaaatgaaa aaagcacaat ttttagcacc
      841 aattatacct tgtcattatt gctgcccctg atgatgtggg gagcaatgcg ctatggttat
      901 aagctgattt cgctgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat
      961 tacattccca tttatcctgg ctataccacg cagctgacca taacctcctc cagttatctg
     1021 gtattctctt ttattgtcaa ttatatggct gtactggcaa ctcgtcagcg agcggtagtc
     1081 agacgcattc agcggcttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc
     1141 ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt gttatttacg catccctggc
     1201 atggaaatgc tggttaagaa ctatggcatc atgctgcgga ttcaa
//
LOCUS       DAZPFP010000391         1238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPFP010000391 DAZPFP010000000
VERSION     DAZPFP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.399"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..555)
                     /locus_tag="ACHUR4_004270"
     CDS             complement(<1..555)
                     /locus_tag="ACHUR4_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1457562.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNY"
     gene            complement(636..1160)
                     /locus_tag="ACHUR4_004271"
     CDS             complement(636..1160)
                     /locus_tag="ACHUR4_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1457563.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
BASE COUNT          356 a          263 c          221 g          398 t
ORIGIN      
        1 ataattgact ttaacattat tagctttgac atccgaaatc gtcacccaat tagctgagtc
       61 attttttatc accaatccat tgccactgcg atttaccagt aactttttaa aggtcgcttt
      121 atttaccgga gcaatacccg ctggccggta aaacaactta attctgtttt gcatcgcaaa
      181 cttcagtgca ttcttgcctt cttgctctgg actatttggt ggaatataca gaacattcag
      241 ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga tttttacttg
      301 ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag gaacctgaat
      361 tttttctggt ggtaacgacg tatcgccatc atcaatccac gcctgcagca gcgaagaacg
      421 atttccctgg ttcatcaact gcaccataac ttctttattt tctgccgggt aaataatgcg
      481 cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa tgagtaaaga
      541 taataatcct ttcatcttaa tgcctcttaa attaaaaagc cgccatgggg atagcggcca
      601 tgtttattac ctggagaaac ttgcaatttt atttattaat cctgcgccac acgcataacc
      661 agagaagata acacatcacc tgattgcact gagttatctg tcagtttcag gtattttgct
      721 ttaaacggaa tagcatattg accttccgct gttttataac tctcaatgtt aaaaggtgca
      781 gaaacatcta ggacatcgct gttatcacct acggtatcgt tgctactggc tatcgcctta
      841 tcgttaccgg ttattttgtc gtttgtgatc aacgagagtt ttacaccagc aacttctttc
      901 gggcagctaa tttttaacat gctgctggtt acaccgactg catcatcata ggtagaatat
      961 ttagctttac cttcaaagac agtggttggt acagtcggca tattaatact tactgaatca
     1021 ccacccgctt cgaatttaca cggagattct gcaatatttc ctttaatggt taatgttccg
     1081 gtatcaacgt ctgcagcaaa tactccagcg ctcataaaaa aagaagaaaa gacagcggca
     1141 gcaataattg aacgtttcat aatatgttcc ttaataaaat ttatttttgg ttgttaagag
     1201 tctgtatctc ttggaaacaa atttctcaca tcaaaaaa
//
LOCUS       DAZPFP010000392         1237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPFP010000392 DAZPFP010000000
VERSION     DAZPFP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.400"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..365)
                     /locus_tag="ACHUR4_004272"
     CDS             complement(<1..365)
                     /locus_tag="ACHUR4_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1457564.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEE"
     gene            679..>1237
                     /gene="icsB"
                     /locus_tag="ACHUR4_004273"
     CDS             679..>1237
                     /gene="icsB"
                     /locus_tag="ACHUR4_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1457565.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSG"
BASE COUNT          408 a          249 c          187 g          393 t
ORIGIN      
        1 atctcttcac ttattacttt attaagctca ttaatatgtc ttttattaac ttcaccaagt
       61 ttataggtat ctaatcgttc catcatggct ttaatttttg acgtatcacc acctgccaac
      121 tttattgcct cgcaaaaaac ttcagctctg tcacgcgcaa acaaaaggct ttttcctgtt
      181 aagcttgttt ggttctgtaa atataatcga ttaagcgctt cgatcccctt atttctttct
      241 ccatttttaa ctctttgata tgaaatcacg cttacacctt tgtgctttcc ggtttcttct
      301 gcgcctttat aggaatctcc gctttgaaat gaaacctgat gcaatcccaa attagttatg
      361 tgcatttaat tatcctcagt cataaatggg gcattgattg cagttttgta cctcgtttta
      421 caacaaaaaa gatacttatt caacagggtt ctctcttttt ctataacact ccgagcctga
      481 tacatcaggc actttatctt gtgggatttc atgatgaaac gagcactaca tatgctcacg
      541 aggtacatat tatgtacagc aaaagaaaat ttgattatgt taacaggctc aagtttctaa
      601 atttattaat tgaatatatt atataccaaa tacattttac atccccacaa tcaccaagta
      661 atggagagtt aataaagtat gatcctcaaa attagcaatt tcattgacgc aagcaataca
      721 aaagggccta tacgcgttga agatacagag catggaccta tattgatagc acagaaattc
      781 aacctaaagg atctcttttt cagaacatta agcactatta atgcaaagat aaattcacag
      841 atattaaatg aacaactaaa aaattatcga ctagagaatc aaaaatcctt attgcttttc
      901 ctcaatacat tagctagcga aaaatctgca gaaagtgcgt ttgccgccta tgaagcagct
      961 aaaaactcaa ttcaacactc tttcacaggt agagacatca aattaatgtt gaataccgca
     1021 gagcgttttc atggcatcgg tacagccaaa aatcttgaaa gacatttagt ttttcgttgc
     1081 tggggaaata gaggcataac ccatttaggg catactagta tcagtataaa aaacaattta
     1141 ctacaggaac caactcatac ctatctcagc tggtatcccg gcgggaatgt tacaaaagat
     1201 acagaaataa actacctttt tgaaaaacgc tcaggtt
//
LOCUS       DAZPFP010000393         1236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPFP010000393 DAZPFP010000000
VERSION     DAZPFP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.401"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            191..>1236
                     /locus_tag="ACHUR4_004274"
     CDS             191..>1236
                     /locus_tag="ACHUR4_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457566.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          402 a          283 c          195 g          356 t
ORIGIN      
        1 atcctctgca ttgtgaatat gtaaaacatc aaaaaaagat gaaccaaata tacgaaaaaa
       61 gcaaatatac ttaccagggt agaaagcatg atataacact cattccctta tggcaaatat
      121 tctgaaacac cctcctcatt aggagagata cattaaatac aatttattca caatctaatt
      181 ccatatccat atgcgagaaa ttaatatgct cagaaacatt tcatcctgtt tatttccaca
      241 tatcagcaca attacatccc ccaaccatta tttgtccgaa tgggatgatt gggagaaaca
      301 ggggttaccg gaagaacagc gtactgaggc ggtaagaaga cttcgtgcat gtcttacctc
      361 taaggggcat aaactggacc tgcgagcctt ggcgctttcc tcgctacctg tactccctgc
      421 ttgcattaaa aagcttgatg tgagctgtaa taaattaacc atccttactg atttacctga
      481 aaatattaaa gaacttattg caagagataa tttcttaaca catatatctg cattaccaca
      541 ttatctaata actttggatg tgtccgaaaa tcaattagag aatctgccgt tattaccaga
      601 caccatcaaa tcactaagcg cagagtataa taggttatcc acactgcctt cattaccctt
      661 gaatttaaaa aaacttgagg ttaggaacaa cgaactgcaa actcttccat ctctgccttc
      721 taatcttaag atacttaagg ttgcgcacaa ccatcttact gaactgcccc ctttacctag
      781 gagactgcaa cttctttttg catatagcaa tagattaagc aacttaccaa acatccaaga
      841 aaatattatc atgagaagat ttttttattt tgaaaacaac caaataacta caatcccgac
      901 aaatcttttt cgtttagatc ctcatataac tattgagatt gcaaataacc ccttatcaga
      961 tcaaactctg ctattcttaa tacagcaaac ttcggttcca aattttaacg ggcctcagtt
     1021 tcgtatttcc ctgtcagacc aaaacagact gtttttacgc cagatgttgc cgcaaaattt
     1081 acattcgcgc catatcagag tcatcactga aggggggcag aactttcaga tcccccctct
     1141 tcccgaaact gtggcagcct ggtttcctga agcagatcgt cgggaggttt ctacacaatg
     1201 gacttctttt tccaccgagg agaattcccg ggcatt
//
LOCUS       DAZPFP010000394         1236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPFP010000394 DAZPFP010000000
VERSION     DAZPFP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.402"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(419..1042)
                     /locus_tag="ACHUR4_004275"
     CDS             complement(419..1042)
                     /locus_tag="ACHUR4_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1457567.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          332 a          261 c          252 g          391 t
ORIGIN      
        1 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
       61 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
      121 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
      181 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
      241 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
      301 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
      361 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
      421 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
      481 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
      541 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
      601 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
      661 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
      721 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
      781 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
      841 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
      901 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
      961 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
     1021 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
     1081 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
     1141 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
     1201 ctcctgtcgc agagaactat tcttatatac cacaaa
//
LOCUS       DAZPFP010000395         1224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPFP010000395 DAZPFP010000000
VERSION     DAZPFP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.403"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            8..700
                     /gene="fimC"
                     /locus_tag="ACHUR4_004276"
     CDS             8..700
                     /gene="fimC"
                     /locus_tag="ACHUR4_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1457568.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            731..>1224
                     /locus_tag="ACHUR4_004277"
     CDS             731..>1224
                     /locus_tag="ACHUR4_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1457569.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
BASE COUNT          350 a          265 c          258 g          351 t
ORIGIN      
        1 attaactatg atgactaaaa taaagttatt gatgctcatg atattttatt taatcatttc
       61 ggccagcgcc catgctgccg gagggatcgc attaggtgcc acgcgtatta tttatcccgc
      121 tgatgctaaa cagactgcgg tatggattaa aaatagccat accaatgagc tctttctggt
      181 caattcgtgg attgaaaaca gcagcggtgt aaaagaaaag tcattcatca ttacaccgcc
      241 actatttgtt agtgaaccca aaagcgaaaa tactttgcgt attatttaca ccggtccacc
      301 gctggcggca gatcgtgagt ctctgttctg gatgaatgtt aagacgatcc cttcggtaga
      361 taaaaatgca ttgaacggca ggaatgtttt gcaactggcg attttatcgc gcatgaaatt
      421 atttctccgt ccaattcaat tacaagaatt acccgcagaa gcgccggaca cactcaagtt
      481 ttcgcgatcc ggtaactata tcaatgttca taatccatca cctttttatg tcaccctcgt
      541 taacctgcaa gtgggcagcc aaaagttggg gaatgctatg gcggcaccca gagttagttc
      601 acaaattccc ttaccctcag gagtgcaggg aaagctgaaa tttcagaccg ttaatgatta
      661 tggttcagta accccggtca gagaagtgaa cttaaactaa ccgaatcatc tgacaatatc
      721 agagctaatt atgaaaatac ccactactac ggatattccg cagaggtata cctggtgtct
      781 ggccggaatt tgttattcat ctcttgccat tttaccctcc tttttaagct atgcggaaag
      841 ttatttcaac ccggcatttt tattagagaa tggcacatcc gttgctgatt tatcgcgctt
      901 tgagagaggt aatcatcaac ctgcgggcgt gtatcgggtg gatctctggc gtaatgatga
      961 gttcattggt tcacaggata tcgtatttga atcgacaaca gaaaatacag gtgataaatc
     1021 aggtgggtta atgccctgtt ttaaccaggt actccttgaa cgaattggcc ttaatagcag
     1081 tgcatttccc gagttagccc agcagcaaaa caataaatgc atcaatttac tgaaagctgt
     1141 acctgatgcc acaattaact ttgattttgc agcgatgcgc ctgaacatca ctattcctca
     1201 gatagcgttg ttgagtagcg ctca
//
LOCUS       DAZPFP010000396         1223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPFP010000396 DAZPFP010000000
VERSION     DAZPFP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.404"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..570)
                     /locus_tag="ACHUR4_004278"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="ACHUR4_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            578..1004
                     /gene="tnpA"
                     /locus_tag="ACHUR4_004279"
                     /pseudo
     CDS             578..1004
                     /gene="tnpA"
                     /locus_tag="ACHUR4_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          316 a          309 c          277 g          321 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta ata
//
LOCUS       DAZPFP010000397         1217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPFP010000397 DAZPFP010000000
VERSION     DAZPFP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.405"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHUR4_004280"
     CDS             complement(108..479)
                     /locus_tag="ACHUR4_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457570.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..1037)
                     /locus_tag="ACHUR4_004281"
     CDS             complement(540..1037)
                     /locus_tag="ACHUR4_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457571.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1066..1217)
                     /locus_tag="ACHUR4_004282"
                     /pseudo
     CDS             complement(1066..1217)
                     /locus_tag="ACHUR4_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT          286 a          316 c          290 g          325 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     1141 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1201 tgtccaagat aagcctg
//
LOCUS       DAZPFP010000398         1210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPFP010000398 DAZPFP010000000
VERSION     DAZPFP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.406"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(74..>1210)
                     /locus_tag="ACHUR4_004283"
     CDS             complement(74..>1210)
                     /locus_tag="ACHUR4_004283"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR1457572.1"
                     /translation="DKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNP
                     GYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIV
                     VHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANAD
                     IEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAA
                     AHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLAD
                     LVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISS
                     DILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          379 a          240 c          202 g          389 t
ORIGIN      
        1 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
       61 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
      121 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
      181 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
      241 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
      301 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
      361 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg
      421 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
      481 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
      541 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
      601 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt
      661 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
      721 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
      781 ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
      841 taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat
      901 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
      961 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac
     1021 tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg
     1081 gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc
     1141 acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata
     1201 ttattttatc
//
LOCUS       DAZPFP010000399         1206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPFP010000399 DAZPFP010000000
VERSION     DAZPFP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.407"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..332)
                     /gene="mobF"
                     /locus_tag="ACHUR4_004284"
     CDS             complement(<1..332)
                     /gene="mobF"
                     /locus_tag="ACHUR4_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFR1457573.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAV"
     gene            414..641
                     /gene="vapB"
                     /locus_tag="ACHUR4_004285"
     CDS             414..641
                     /gene="vapB"
                     /locus_tag="ACHUR4_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR1457574.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            641..1039
                     /locus_tag="ACHUR4_004286"
     CDS             641..1039
                     /locus_tag="ACHUR4_004286"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR1457575.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          302 a          336 c          297 g          271 t
ORIGIN      
        1 tccacggcct ggttatgtgc ttcaataaga cgtttgtccc ccccgagcat ggccatcatg
       61 gagatacttt tgggagcgga gaaggtcagg tcgtagccgg gacgatgcct gttactgcca
      121 tcctgcatgc ggcttaaatc cgccccgtcc ggcagcctgc cctccagaag gcgggtaaag
      181 acatccttat cgacactgcc ctgcagcccc agttgttcag ccccctggcc ggcccagcgt
      241 tctcccatgc tgcccagcac atagtaatta tccttgtcgg tataataatt accggcactt
      301 ccggccgatc tgacctgcgc gatactcatc ataaacgtat atccctttga catatcccgg
      361 tatcaatccc acaatagata tacacaagac atatccacat aaggaggcaa ataatggaaa
      421 ccaccgtatt tctcagcaac cgcagccagg cggtcagact gccaaaagcg gttgcattgc
      481 cggaaaacgt aaaacgcgtt gaagtgattg ctgtcggacg aaccagaatc attacgccag
      541 ccggagagac atgggacgaa tggttcgacg gacacagcgt cagcaccgat tttatggata
      601 acagggaaca gcccggcatg caagaaaggg agtcattctg atgctgaagt ttatgctcga
      661 taccaacatc tgcattttta cgataaagaa caaacccgcc agcgtcaggg agcgttttaa
      721 cctgaaccag gggaaaatgt gtatcagttc ggtcaccctg atggagctga tatatggtgc
      781 agaaaaaagc cagatgcctg aacgtaatct cgctgtgatc gagggatttg tttcccgcat
      841 tgatgttctg gattacgacg ctgctgcagc cacacacacc ggccagataa gagcagaact
      901 tgcccgtcag ggacgccctg tcgggccatt tgatcaaatg atcgcaggtc atgcccgcag
      961 tcgggggctg attattgtga ctaataacac ccgggaattt gaacgtgtgg gcggcctgag
     1021 aactgaagac tggagctgac ctgttcatca gaaatcatct cccggctcaa catcctcccc
     1081 gcgttcccgg tgcacattga tgttcacctc ttcacgacgc tgcatctgct gccaggtgtc
     1141 cggatgattt tcctgctgcc atgcctcata agcggccata tccaccactt caccggattc
     1201 actgat
//
LOCUS       DAZPFP010000400         1199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPFP010000400 DAZPFP010000000
VERSION     DAZPFP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.408"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(123..>1199)
                     /locus_tag="ACHUR4_004287"
     CDS             complement(123..>1199)
                     /locus_tag="ACHUR4_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1457576.1"
                     /translation="VVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDG
                     DKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGS
                     LDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLN
                     FAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQER
                     AAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNKAQSFEVVA
                     QYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYVDYKINLID
                     ESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          334 a          268 c          263 g          334 t
ORIGIN      
        1 gaataatatt aattgaggtg aatgaccaga aatatcaaga attgctcatt tttacttaag
       61 gagtcataca gcagcactta aatcaaaaac cagcccagag aagggctggt caggagagga
      121 cgttagaact gataggtaat gcccgtagcg acgatgttat cggtgcgaat atctacggca
      181 cgggtaaagt cgctttcatc aatcaggttg attttataat caacataggt cgacatattt
      241 ttgttgaaga aatacgtcgc accaacgtcg atatactcaa tcatgtcctg gtcggcgtag
      301 cggcccagat ctctgccttt cgatttcagg taagcgagtg atgggcgcaa gccgaagtcg
      361 aattgatatt gcgccaccac ttcaaaactt tgtgctttgt tagagatata accatcccca
      421 taggtggtca tgtttaatgt ttcatcgtaa ttagctgcaa agtagatgtt gttggcatca
      481 tactttatac ctgtggccca tagttctgca ttcttaccgg aggcattgag aggattagct
      541 gcggctcttt cctgcgcatt tgtccgatcg gatttggtat ataccgcacc gataccaaag
      601 ccatcataaa cataagttga ggagataccg aaaccgtcac cgttggcacg cgtgtcgtca
      661 gcaccataaa gatgactgtt gtcagtacgt ttatttttgc cttgatattg cgcggcaaag
      721 ttcagccctt caattaagcc aaagaaatcg tagttgcgat aggttgctag accggtggta
      781 cggtatgtca tgaagacatc tttctgactc caggtatcgc caccaaattc tggcaacata
      841 tcggtccatg aaccaatgtc gtagccgaca ccgtagttac gaccatagtc cagagagcca
      901 aattcactaa tttttaaacc agcataggcc agacgggtct tgttgccgcg cgagccgttg
      961 gcttcatcgt tatagccttt aaaatcatat tcccagtgac caaaaccaat catttgatca
     1021 ttgatttgcg tttctccttt aaagccgaga cgggcataag ttttatcacc atcgtcacgc
     1081 ttatcatcag taaaataacg tagagcggta accttgccat aaaggtcgag tttattacca
     1141 tccttgttat agacttcggc agcgtgtgct acgttcgcag ctaacaaacc agttacaac
//
LOCUS       DAZPFP010000401         1191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPFP010000401 DAZPFP010000000
VERSION     DAZPFP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.409"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            15..638
                     /gene="dctP"
                     /locus_tag="ACHUR4_004288"
                     /pseudo
     CDS             15..638
                     /gene="dctP"
                     /locus_tag="ACHUR4_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
     gene            683..1186
                     /locus_tag="ACHUR4_004289"
     CDS             683..1186
                     /locus_tag="ACHUR4_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1457577.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT          347 a          250 c          258 g          336 t
ORIGIN      
        1 gccgttctta ttaagttgtt tttggtgaag ttggtaaaga aattatggac tcaacaaaag
       61 acaaaggctt ttttgcttta tcagcttatg tagcgggtac tcgcagtttc tatgcgaaaa
      121 aaccgattac aaagccagaa gatcttaaag gactgaaaat tcgtgttcag ccaagtccga
      181 ccaccatcaa aatgattgaa ctgatgggtg gttcacccac tccaatttca tttggcgaag
      241 tttatactgc gatgcagcaa ggtgtagtag atggcgcaga aaataacgtg ccttcctgga
      301 tgcagactcg acatattgaa attgccaaag ttttttctga agatgaacac gcctcaattc
      361 ctgacttttt ggttatctcc accaaaacat ggaataaatt aaccccagaa cagcaacaaa
      421 tcctcgaaac agcggcgaag aaatccgaag cttatcaaca aaaattgtgg gaaaaaattg
      481 acgctgacac acgtgctcag gcgaaagcta tggggggtga aattgtaaaa gtggataaag
      541 cgccattccg taaagccgtg caacctctgt ttgacgactt caaaaaagat ccaaaacaag
      601 ctgctttgtt agagaaattc gacaacgctg ctcaataata ttccgggcca gcctctggcc
      661 ctgattttcc aggatgctta caatgatatt taaccgcctg aaacttgcgg tagaccgcgt
      721 cattgccacg ttttctgtcg ccgtaatgct tgctctggtt gtatgcgtag tctggcaagt
      781 atttagccgc tacgtactta accagccaag tacattaacc gatgagctgg cacgcttttt
      841 gatgatctgg gtaggattat taggtgctgc atataccgtc ggcgcgcagc gacatctctc
      901 tattgattta tttgctctcg ccctgaataa gcgcaaacaa ttacttctta gtatagtcat
      961 taacgttctc attttaggtt ttgccggttc ggtcattgtg actggtggcc tgaaattaat
     1021 tgataaaaca ctggcaacat cacaggtatc tgccgctatg caaataccga tgggatacgt
     1081 atatatcatt ctgccattaa gtggagtggt aatgatgttt tatgccttgt gttttattaa
     1141 tcaaagcatt caacaactga aacaacctgc gcaggaggcc agttaatgga t
//
LOCUS       DAZPFP010000402         1190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPFP010000402 DAZPFP010000000
VERSION     DAZPFP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.410"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>1190
                     /locus_tag="ACHUR4_004290"
     CDS             <1..>1190
                     /locus_tag="ACHUR4_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1457578.1"
                     /translation="DREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSIT
                     APDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIP
                     DYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPG
                     EAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQP
                     EDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRA
                     KISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEW
                     KNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIER
                     SITPP"
BASE COUNT          430 a          241 c          221 g          298 t
ORIGIN      
        1 gacagagaaa ttatatcaac ttttcacgca gaaaaagtca ttaatgatct gttaaaaaac
       61 gattataaaa tttccctttc tccagataca acttaccgag agctgaataa agctgcacag
      121 agtagcatta cagcgccaga caggatagga gaagggaaaa catgggttta tcaacgagat
      181 acaatggttg aaagaggtga taacagtggt gttcatcagt atggtccagc tgaacatttc
      241 actcacatta tatctgacaa accgtcccca aaagataagt atgttgcata tgctattaac
      301 attcctgact atgagctagc agccgatgta tataatatta acgtgacgtc accttccgga
      361 cagcaagaaa catttaaaat actaatcaat ccagaacatc tacggcaaac acttgagcgt
      421 aaatctctta ctgctgttca gaaatcacaa tgtgaaatca tcacccccaa aaaacctggc
      481 gaagcgattc ttcatgcttt taatgccacc taccagcaaa tcagggaaaa tatgtctgag
      541 tttgcacgtt cccattatgg gtatatacaa atccctccag tgacaacttt ccgcgccgac
      601 ggaccagaaa ctcccgaaga agaaaaaggt tactggtttc acgcttatca acccgaagat
      661 ctttgtacca tccataatcc aatgggggat ttgcaggatt ttatcgcatt ggttaaagat
      721 gctaaaaaat ttggtatcga tatcattcct gattatacct ttaactttat gggaatcggg
      781 ggaagtggta aaaatgacct ggattatccc tctgctgata tacgagcgaa gatcagtaaa
      841 gatatagaaa gtggtatccc tggctattgg caaggtcagg ttttgattcc attcattaaa
      901 gatccagtaa caaaagaacg taaacaaatc catccagaag atatacatct cactgcaaaa
      961 gacttcgaag caagtaaaga taacatctct aaggatgaat ggaaaaacct ccatgcatta
     1021 aaagaaaaga gtttaaatgg aatgcctaaa acaaccccca aaagtgacca ggttattatg
     1081 ttgcaaaatc aatacgttcg tgaaatgcga aaatatggcg tacgaggttt acgttatgat
     1141 gcagcaaaac actcaaaaca tgaacaaata gaaagatcaa taaccccacc
//
LOCUS       DAZPFP010000403         1167 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPFP010000403 DAZPFP010000000
VERSION     DAZPFP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.411"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(176..298)
                     /locus_tag="ACHUR4_004291"
                     /pseudo
     CDS             complement(176..298)
                     /locus_tag="ACHUR4_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            441..995
                     /locus_tag="ACHUR4_004292"
                     /pseudo
     CDS             441..995
                     /locus_tag="ACHUR4_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT          272 a          282 c          297 g          316 t
ORIGIN      
        1 tcctaatata ttgaattgtt gtatagccag tttttgtatt ttcctagctc atggatgtgg
       61 gatccctctc ccctttctct gctcaaaggt aagcggctcg tcagaatcat attgatattt
      121 actgagaagt cagatcaact ttccaaggca acagatcgcg tacccggttt gccggtcagt
      181 cctggatatg ttcaataacg taacgcagcc atttttctgg ctccacattg ttcagacggc
      241 atgtgccgat cagcgagaac aacaccgctg catgttcgtc accgctgtcg gaacctgcga
      301 gtcgagtcag ccggagagag tttcactctc cggctgctta cggaaccgta cgtgacactc
      361 tcgcgtcata cggctcttga tcactcattc gatgcacgta gtaaatacca gtgagcaaat
      421 tgatcttacc cagcaatacg gtcagttacc gcgcacatgg cctgcctgtg tcagaaaatc
      481 tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga gacatcacgt
      541 acttacgtac agatgaaggc tggctgtatc tggctgtggt cattgacctg tggtcacgtg
      601 ccgttattgg ctggtccatg tcaccacgcc tgacggcgca actggcctgc gatgccctgc
      661 agatggcgct gtggcggcgt aagagacccc ggaacgttat cgttcactcg gaccgaggaa
      721 gtcagtactg ttcagcagat tatcaggcgc tgctgaagtg gcataatctg cgtggcagta
      781 tgagcgcaaa gggctgctgc tacgataatg cctgcgtgga aagcttcttt cattcgctga
      841 aagtggaatg tctccacgga gaacacttta tcagccggga aataatgagg gcaacagtgt
      901 ttaattatat cgaatgtgat tacaatcgct ggcgacggca cagttggtgt ggcggcctca
      961 gtccggaaca atttgaaaac cagaacctcg cttaggcctg tgtccatatt acgtgggtag
     1021 gatcacttac gccctgtggt tgaaacgtga gcatattgtt ttcagatgct gttgtcttgc
     1081 ttcagagtga tctgtcctcc ggatttacgc aaagttatcc acaggcactg tgcatttaaa
     1141 aaacttatct tttcttcttg attttaa
//
LOCUS       DAZPFP010000404         1165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPFP010000404 DAZPFP010000000
VERSION     DAZPFP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.412"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>1165)
                     /locus_tag="ACHUR4_004293"
     CDS             complement(<1..>1165)
                     /locus_tag="ACHUR4_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1457579.1"
                     /translation="PKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVRNTTSVL
                     SGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLVNFLIGY
                     IPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLSHNVGGA
                     LAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGLPPIEEW
                     KGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIVSWAPKF
                     LSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNTLFLALT
                     IILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAGAA"
BASE COUNT          381 a          273 c          237 g          274 t
ORIGIN      
        1 atgcggctcc ggcagcttct ttaggaacaa gctccattga ataaagacca ataaacatca
       61 ccggaccata cactgccagc cccaacagaa taaggattgt atagtttagc cagagatatt
      121 cattgcctga tggcacgtac cagtacgcga ttaataaaat aatggtaagt gccagaaata
      181 aagtatttgt taatggtttt gagtttctga acagtctgac agacaggtat ccggcaagaa
      241 gcataccagc cacaccaccg atttcaaaaa cagaaccccc ccccattgat gccagttttc
      301 ccatatcctt accaccgaca tccagactgt cacttaaaaa cttaggagcc caggaaacaa
      361 taccgtatct cagaatataa acaaacgaac catagataca gcagtaaatg agtttattat
      421 ttcggatgat atgttttctg atgatatcag gaatggagag tctcggacct tgttcacggc
      481 ctttagccgg gtgtgaaacg acgttacctt tccattcttc aatgggagga agccccactg
      541 atacaggtct gtccacctga ataaagtaaa tcagcacact cattgccata cacagtactc
      601 ctgggaagat aaatgcggcc cgggcatgat ccagagcact gtcgccgaaa aaaccaaaac
      661 tgaaaccaat cagaacgggg gccagagctc ccccgacatt gtgcgataaa ttccacagtg
      721 tggcccaggt gatcctttcc cgatgttcat accagaacac cagtgttttg gcaccaggag
      781 gatatcccat accttggatc cagccgttaa gtaaatacat tgttgaaaat aacgtaacgg
      841 atgtgatgat cccgggaata tatcctatca gaaaatttac cagtccggac aggaataaac
      901 caagagacaa aaatactttt gaattacttc tgtcagaaat aagtccggaa acaaactttg
      961 atataccata ggataaaaaa ccggcacagg agataatgcc gatctcggtt gcagacatgt
     1021 gcagcacacc ggataacaca ctggtggtgt ttcggacaaa ataatacccc atatatccga
     1081 ggaaaatgct gataaagacc ctgtatcgca ggacgggata taatcgttca atctcttcag
     1141 agggtaatct ttcaatttcc ttagg
//
LOCUS       DAZPFP010000405         1159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPFP010000405 DAZPFP010000000
VERSION     DAZPFP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.413"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUR4_004294"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUR4_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1457580.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAZPFP010000406         1158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPFP010000406 DAZPFP010000000
VERSION     DAZPFP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.414"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            24..746
                     /gene="phoC"
                     /locus_tag="ACHUR4_004295"
     CDS             24..746
                     /gene="phoC"
                     /locus_tag="ACHUR4_004295"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR1457581.1"
                     /translation="MVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAIDSLALLPPPP
                     QIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFGSPITAKDSP
                     ELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSRNGSYPSGHT
                     SIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIVGSAIVATLH
                     SNPVFQAQLQKAKDEFANNQKK"
     gene            complement(801..905)
                     /locus_tag="ACHUR4_004296"
                     /pseudo
     CDS             complement(801..905)
                     /locus_tag="ACHUR4_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(916..>1158)
                     /locus_tag="ACHUR4_004297"
     CDS             complement(916..>1158)
                     /locus_tag="ACHUR4_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457582.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          306 a          264 c          253 g          335 t
ORIGIN      
        1 aaagacagct ttttactctt agtattgtcg gggtattttc tttaaatacc tttgcatcat
       61 tccctccggg aaatgatgtg acaacaaaac ctgaccttta ctacctgaca aacgataatg
      121 ctattgacag tctggcatta ttaccgcctc caccacaaat cggaagtatt gccttcctga
      181 atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa tactgaaaga gggaagctgg
      241 cagctgagga tgccaatctc agtagtggtg gcgtggcaaa tgtattttct gctgcttttg
      301 gttctccgat aacggctaaa gattcaccag aattgcataa attactgaca aatatgattg
      361 aggatgccgg agacctggca acgcgttcag caaaagaata ttatatgcgt attcgacctt
      421 ttgcttttta tggtgtttct acgtgtaata caaaagaaca ggatacatta tccagaaatg
      481 gctcttatcc atcaggtcat acatcgattg gttgggcaac agcacttgta ttgtccgaaa
      541 taaatccggc acgccaggat accattctga aacggggtta tgaactgggg gacagcaggg
      601 ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc acgtattgtt ggctctgcta
      661 ttgtagcaac actgcactca aatcctgtgt tccaggcaca attacaaaaa gcgaaagatg
      721 aattcgctaa caatcagaaa aaataatgct tttagccagt acgccttata accccgacct
      781 tcagggctaa tgccagtcag ttaagcaact gactggctct ttttcagggc tgtggggtat
      841 ttccagtgcc tctcctttac cactctcgga aaggcccttt cccttcttgt cggtaatttc
      901 actaggctgt gtcccttaat tacgtaagcg ttgataaaag agtcggatgc agcccagttt
      961 cactatcgcc aggtaatttc tcgccgtttt gtcgtaacgc gtggcgatgc ggcgatattc
     1021 tttcagccgc ccaaagcacc gttcaacgac attacgattg cagtacgcat cacgatcaag
     1081 ctgtgcacgt ccatccgatg ccattttctc atttgacttt cgggggatta ctgcctttat
     1141 accgttattt ttcagctc
//
LOCUS       DAZPFP010000407         1156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPFP010000407 DAZPFP010000000
VERSION     DAZPFP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.415"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHUR4_004298"
     CDS             complement(<1..1103)
                     /locus_tag="ACHUR4_004298"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1457583.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          274 a          303 c          302 g          277 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgca
//
LOCUS       DAZPFP010000408         1144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPFP010000408 DAZPFP010000000
VERSION     DAZPFP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.416"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            13..837
                     /gene="gadX"
                     /locus_tag="ACHUR4_004299"
     CDS             13..837
                     /gene="gadX"
                     /locus_tag="ACHUR4_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1457584.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
BASE COUNT          339 a          213 c          244 g          348 t
ORIGIN      
        1 attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc aagacataaa
       61 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
      121 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
      181 gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct tcatgcacat
      241 aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
      301 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
      361 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttttg
      421 aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
      481 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag cctgttaaag
      541 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgcaa
      601 cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc agtgtcctgt
      661 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
      721 acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt
      781 gttgcgcaag ggaattttta cggcactaac cgttctgcgg aaggaataag attatagagt
      841 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
      901 ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta
      961 agttaaatat aacttttaca ggaaataaga tcagccattt ttttataaac ataagctata
     1021 cgctgtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
     1081 ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt
     1141 ctgt
//
LOCUS       DAZPFP010000409         1144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPFP010000409 DAZPFP010000000
VERSION     DAZPFP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.417"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..384)
                     /gene="ospG"
                     /locus_tag="ACHUR4_004300"
     CDS             complement(<1..384)
                     /gene="ospG"
                     /locus_tag="ACHUR4_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR1457585.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICK"
     gene            complement(467..636)
                     /gene="yacB"
                     /locus_tag="ACHUR4_004301"
                     /pseudo
     CDS             complement(467..636)
                     /gene="yacB"
                     /locus_tag="ACHUR4_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(636..905)
                     /gene="yacA"
                     /locus_tag="ACHUR4_004302"
                     /pseudo
     CDS             complement(636..905)
                     /gene="yacA"
                     /locus_tag="ACHUR4_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
BASE COUNT          298 a          290 c          241 g          315 t
ORIGIN      
        1 tttacatatc aactgtagat aaaggctctc tatattatca gggatatcag ctgtatcaat
       61 gtcactaagg ggagtcccag gcacgcgcag cattcggagg tacacatctc cgccatactg
      121 tataacaacg gatgcttcat cgccataaaa agcattaaaa agctcagatt cttgccaagc
      181 catttccaga atctcattgt actggttgcc aataagatca tactttttat acaaagcaga
      241 tgaatcattc acatcttcaa agatttctgc tgtcgacccc tgacctatta acttaccgag
      301 aatgggctcc gttactatgc cagcatgaga attattattc tcaaatggaa aaggtgtttg
      361 aataatggta gatgttattt tcatgtcata cctttgttac gatatttaaa aatcacaaga
      421 gattttcaca caaaataacg ttacacgcta ccttgtgtgg tccagatgtt ccttccacac
      481 gaccgttgcg gcctttatac ggctgccctg ccaggctgct ggctgaataa ccaatccgtt
      541 cgtccggttc aatggcggcc atcaggttct gctccgcaat gtactacgaa tgcgcttcct
      601 gtcctggatg gccagcatgg tccagaacat ctccatcatt tatcagcaat cttcctgctc
      661 atttcatccc gccatgcagc agcagaggat tcgaccatgt catggggaag cacatcaccg
      721 cattcgagtt gctgacggcc tgccgcaacc tagtcacgga accaagaatt gtgctgccgt
      781 tcaacggtct ggcgcataaa atcccggatt aactgagagc cattacggtc catgcttttt
      841 gcagcggcca taaaagcatc cttcagttct gcgtcgattc ttacactcat attaacctgt
      901 gccatttgcc acctcgtcat taccacgtat atacacagta tataactgtt cagatatcgt
      961 caccaggata tgccgcgcca gcggcatgta aggcggcact ccgttgttgc atatggcaga
     1021 ggagtaaaac cgccgaagcc cggcgtaagc cggtacgtat tgacaggttt cacctgtcct
     1081 atcactggcg ctgacagttt tatgcgatgt gattatgaat ggtgcagttg tgagccgttt
     1141 tcag
//
LOCUS       DAZPFP010000410         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPFP010000410 DAZPFP010000000
VERSION     DAZPFP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.418"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHUR4_004303"
     CDS             <1..730
                     /locus_tag="ACHUR4_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR1457586.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHUR4_004304"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHUR4_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZPFP010000411         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPFP010000411 DAZPFP010000000
VERSION     DAZPFP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.419"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            13..>1115
                     /locus_tag="ACHUR4_004305"
     CDS             13..>1115
                     /locus_tag="ACHUR4_004305"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1457587.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          263 a          291 c          302 g          259 t
ORIGIN      
        1 aaggagttta aaatggataa gaagcaagta acggatttaa ggtcggaact actcgattca
       61 cgttttggtg cgaagtctat ttccactatc gcagaatcaa aacgttttcc gctgcacgaa
      121 atgcgcgacg atgtcgcatt tcagattatc aatgatgaat tatatcttga tggcaacgct
      181 cgtcagaacc tggccacttt ctgccagacc tgggacgacg aaaacgtcca caagttgatg
      241 gatttatcca ttaacaaaaa ctggatcgac aaagaagaat atccgcaatc cgcagccatc
      301 gacctgcgtt gcgtaaacat ggttgccgat ctgtggcatg cgcctgcgcc gaaaaatggt
      361 caggccgttg gcaccaacac cattggttct tccgaggcct gtatgctcgg cgggatggcg
      421 atgaaatggc gttggcgcaa gcgtatggaa gctgcaggca aaccaacgga taaaccaaac
      481 ctggtgtgcg gtccggtgca aatctgctgg cataaattcg cccgctactg ggatgtggag
      541 ctgcgtgaga tccctatgcg ccccggtcag ttgtttatgg acccgaaacg catgattgaa
      601 gcctgcgacg aaaataccat cggcgtggtg ccgactttcg gcgtgaccta caccggtaac
      661 tatgagttcc cgcaaccgct gcacgatgca ctggataaat tccaggccga caccggtatc
      721 gacatcgaca tgcacatcga cgccgccagc ggtggctttc tggcaccgtt cgtcgccccg
      781 gatatcgtct gggacttccg cctgccgcgt gtgaaatcga tcagtgcttc aggccataaa
      841 ttcggtctgg ctccgctggg ctgcggctgg gttatctggc gtgacgaaga agcgctgccg
      901 caggaactgg tgttcaacgt tgactacctc ggtggtcaga ttggtacttt tgccatcaac
      961 ttctcccgcc cggcgggtca ggtgattgca cagtactatg aattcctgcg ccttggtcgt
     1021 gaaggctata ccaaagtaca gaatgcttcc taccaggtcg ctgcctatct ggcggatgag
     1081 atcgccaaac tggggccgta tgagttcatc tgtac
//
LOCUS       DAZPFP010000412         1114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPFP010000412 DAZPFP010000000
VERSION     DAZPFP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.420"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..710
                     /locus_tag="ACHUR4_004306"
     CDS             <1..710
                     /locus_tag="ACHUR4_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR1457588.1"
                     /translation="KPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIRQYPA
                     YYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDKFHIS
                     IAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSDQECS
                     QYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGSE
                     RQEQMLREEPFYRLMIE"
BASE COUNT          325 a          211 c          232 g          346 t
ORIGIN      
        1 aaaagccctg tctaaaacta aatttagatt ctttgaatgt tgtgaggtct gaaataccgc
       61 agatgctttc tgcgaatgaa agattgaaaa ataacttcaa tatattgtac aaccaaatac
      121 ggcaataccc agcgtactat ttcaaagtag catcgaacgt gccaacttac tctgatatat
      181 gtcaatcttt ttctgtcatg taccaaggat tccagattgt gaaccatagt ggagatgtgt
      241 ttattcatgc atgtcgtgaa aatcctcaga gtaaggggga ttttgttggg gacaagtttc
      301 atattagtat agctagggag caagttcctc tggcattcca aattttgtct ggtttattat
      361 tttcagagga cagtcctata gataaatgga agataactga tatgaatcga gtttctcagc
      421 aatctcgtgt ggggatagga gctcagttta cgctatatgt aaaatcagat caggaatgct
      481 cgcaatatag tgctttatta cttcataaaa tacgacaatt tataatgtgt ctcgagtcta
      541 atctattaag aagcaaaatc gctcccgggg agtatccggc gtcagatgtt agacctgaag
      601 actggaaata tgtcagctat cgtaatgaac tacgaagcga tcgagatgga agtgaaaggc
      661 aagagcaaat gttacgagag gaaccatttt atcgtttgat gatagagtag agaagatgcc
      721 agctacacca ccttgtagct tgaaaactgg ttagaataac tacgattaga tttggcactg
      781 cgtatgcagc ctgattcctg cccgtgacag agcattcggc tcataaactg ctgagctact
      841 catctgaaat ccgaacagtt gtccaccatt tcagcgtgca tctcctgttt gagtcgtttg
      901 attgcttttc gggatattaa cctgtcggat tttgcattag ttaatgctcc catcaggcct
      961 tcatcttttc cagtgcctat tttcctgttc tccttttgca caatataatc agatcatctg
     1021 tatcgttgac gagctcgcta tcttatagga gcattctgaa taagctacct ccttgtaagg
     1081 aggcttttac tattagtaac tgcaatctaa tagt
//
LOCUS       DAZPFP010000413         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPFP010000413 DAZPFP010000000
VERSION     DAZPFP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.421"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHUR4_004307"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHUR4_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHUR4_004308"
     CDS             992..>1109
                     /locus_tag="ACHUR4_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457589.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZPFP010000414         1107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPFP010000414 DAZPFP010000000
VERSION     DAZPFP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.422"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(99..998)
                     /gene="lysO"
                     /locus_tag="ACHUR4_004309"
     CDS             complement(99..998)
                     /gene="lysO"
                     /locus_tag="ACHUR4_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1457590.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT          369 a          253 c          277 g          208 t
ORIGIN      
        1 agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag attgatttag
       61 cgcaattttg gcggcaggga actaccgcca gagaggtatt acgcagagaa aaaggcgatg
      121 aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg gaccatatcc
      181 agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc accgcataag
      241 cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat agcaatcagt
      301 tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa agattcggtc
      361 aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag cgcggtattg
      421 atggggagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact gacaaccacc
      481 accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt catgccatta
      541 ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc actggcttcg
      601 gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat cactactacg
      661 ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt ttcttgctga
      721 tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat attacacagt
      781 aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt actggcgagg
      841 ttatcgagaa acgccagact gatacccata aaaaagagaa taaggtaaac catccagctt
      901 aatagctgat taatagcttt taacgcagct tgttggcgaa gcggaatgag gtaacccaca
      961 atcaggggaa ccagaatgat taacagccca gaaaacatga aaatccagtc cttgcaaaga
     1021 agaagtcgaa atgcgcgatg acacactact gaaagcggaa gggcgagtaa agttgcaatt
     1081 aaaaggaaat gttatgcatc agagggg
//
LOCUS       DAZPFP010000415         1101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPFP010000415 DAZPFP010000000
VERSION     DAZPFP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.423"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            394..864
                     /gene="argR"
                     /locus_tag="ACHUR4_004310"
     CDS             394..864
                     /gene="argR"
                     /locus_tag="ACHUR4_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1457591.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          325 a          255 c          207 g          314 t
ORIGIN      
        1 ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt
       61 gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg
      121 taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta
      181 ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaaa cagaatttgc
      241 atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata
      301 acattatttc agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa
      361 taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa
      421 ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc
      481 gtcgccgcgt tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg
      541 ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg
      601 ccagctgaac tgggtgtacc aaccacctcc agtccattga agaatctggt gctggatatc
      661 gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct
      721 cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac
      781 accatcttta ccacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta
      841 gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt
      901 tgcttatccc tctcattccc cttctttctc ctgcgataca tttaacgaat agtgcgtttt
      961 accgcgacac atcattcaca aaatgaatac atacggtaaa aaaagcacat tatgcaaaat
     1021 tcattatcta attgaaaata ctataattaa taataaataa ccgtgttttt tatacttttt
     1081 tgttattaaa attcacattt t
//
LOCUS       DAZPFP010000416         1094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPFP010000416 DAZPFP010000000
VERSION     DAZPFP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.424"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(213..335)
                     /locus_tag="ACHUR4_004311"
     CDS             complement(213..335)
                     /locus_tag="ACHUR4_004311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457592.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(408..>1094)
                     /gene="phoN2"
                     /locus_tag="ACHUR4_004312"
     CDS             complement(408..>1094)
                     /gene="phoN2"
                     /locus_tag="ACHUR4_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR1457593.1"
                     /translation="PSANALKAEGFLTQQTSPDSLSILPPPPAENSVVFQADKAHYEF
                     GRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTPILYQLLTQVLQDSHDYAV
                     RNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHASFGWAVALILAEINPQRK
                     AEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLHNTPEFTKSLSEAKKEFEE
                     LNTPTNELTP"
BASE COUNT          283 a          239 c          245 g          327 t
ORIGIN      
        1 attgattttt ttattaaaaa atggagcggt atcaggcaaa cgatttgaga ggtgacctaa
       61 aatatattta ggttgtgccc tgaatctgaa gttgtcattt ctgttatatt gatcgacata
      121 ggggatggtg cagaccgttc ccctgcaaca ccacattgca tgtggtgaac ggaaccgccc
      181 cgagtttcct agagaatgtt ttatctgtga actcaggttg ccagatcatc gtttccgatg
      241 gcagcataat aagctttttc tgcttctgcc ggagggatat gtcccagcct ttccagtaat
      301 cccttaaatt caggggtaaa acgtggtgtg cccatagact cctcctatgc tcatataggg
      361 tagatttgtc tatgggccct ccatagcctg atacaggctg tccagcttta tggggtcagt
      421 tcattggtag gagtatttaa ttcttcaaac tcttttttgg cttcgctaag gcttttggta
      481 aattcaggtg tattatgaag tactgcaaca accgatgctc ccattaaacg cccagcctct
      541 acatcgcttt gccaatgcgc accgcagatg acccgacttt ctccaaactc atatccacgt
      601 cgaagtattt ccgctttacg ttgaggatta atctccgcaa gtatcagtgc tactgcccaa
      661 ccaaaggatg catgaccaga gggataagag ccagtgatag ccattttctc atctttatca
      721 ggtgtacagg ttgcgtcttt ataaataacg aatggacgaa ctcttttata atattctttg
      781 gcgttacgca cggcgtaatc atggctatcc tgtagtactt gtgttaacaa ctgatataag
      841 attggggtat tttcccttga aatatccatg ccataagcat ctgaaaatgc aagaccaaaa
      901 ttctcgtagt atgcatcttc gctagcgaga cgtacacgat tagcatcccg gagcgagcgg
      961 ccgaattcat aatgagcttt gtcagcctga aatactactg aattctctgc cggaggcggc
     1021 ggaagtattg acaaactgtc tggtgaagtt tgttgagtga gaaaaccttc tgccttcaga
     1081 gcatttgctg aggg
//
LOCUS       DAZPFP010000417         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPFP010000417 DAZPFP010000000
VERSION     DAZPFP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.425"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUR4_004313"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUR4_004313"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1457594.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHUR4_004314"
     tRNA            complement(986..1062)
                     /locus_tag="ACHUR4_004314"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZPFP010000418         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPFP010000418 DAZPFP010000000
VERSION     DAZPFP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.426"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHUR4_004315"
     CDS             <1..1038
                     /locus_tag="ACHUR4_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1457595.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
BASE COUNT          297 a          264 c          288 g          236 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgc
//
LOCUS       DAZPFP010000419         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPFP010000419 DAZPFP010000000
VERSION     DAZPFP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.427"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHUR4_004316"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHUR4_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR1457596.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DAZPFP010000420         1069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPFP010000420 DAZPFP010000000
VERSION     DAZPFP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.428"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(227..652)
                     /locus_tag="ACHUR4_004317"
     CDS             complement(227..652)
                     /locus_tag="ACHUR4_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1457597.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHUR4_004318"
     CDS             complement(649..1032)
                     /locus_tag="ACHUR4_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1457598.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZPFP010000421         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPFP010000421 DAZPFP010000000
VERSION     DAZPFP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.429"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUR4_004319"
     CDS             <1..560
                     /locus_tag="ACHUR4_004319"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR1457599.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHUR4_004320"
     CDS             787..>1068
                     /locus_tag="ACHUR4_004320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR1457600.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZPFP010000422         1055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPFP010000422 DAZPFP010000000
VERSION     DAZPFP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.430"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>1055
                     /gene="ospD3"
                     /locus_tag="ACHUR4_004321"
     CDS             <1..>1055
                     /gene="ospD3"
                     /locus_tag="ACHUR4_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1457601.1"
                     /translation="IKETKNGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNF
                     LDEKHQKCYGLISDGMSIFVDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQK
                     VTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDK
                     TVELLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYD
                     EVHRQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLAL
                     NNGQADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKI
                     LHTADLTPHQASKLLAA"
BASE COUNT          356 a          197 c          209 g          293 t
ORIGIN      
        1 gattaaagaa actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac
       61 agcaactcat ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac
      121 tgtagataat tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat
      181 gtctatattt gtggacagac atactccaac aagcatgtcc tccataatca gatggcctga
      241 taatttactt caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat
      301 ccaaaaagta acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact
      361 tttggcagcc aaaaatgacg atggtttgtc aggattgctt ttagctttac aaaatgggca
      421 ttcagataca atcttagcat acggagaact cttggaaact tctggactta accttgataa
      481 aacggtagaa ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc
      541 acttcaaaat gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc
      601 aataaatatc gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt
      661 acacagacag atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg
      721 cgcatacggt gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt
      781 gctggcctca aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca
      841 ggctgatgca atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa
      901 aaaagcagag ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt
      961 gcataatgga tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct
     1021 tactccacat caggcatcaa aattactggc agcag
//
LOCUS       DAZPFP010000423         1034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPFP010000423 DAZPFP010000000
VERSION     DAZPFP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.431"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..882)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUR4_004322"
     CDS             complement(<1..882)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUR4_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR1457602.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GPQIYFSMSDGQQNTLHR"
BASE COUNT          302 a          174 c          241 g          317 t
ORIGIN      
        1 gcgatggagt gtattctgtt gtccgtcact catggagaag taaatctgcg ggccgtggta
       61 gtccggcgaa gaggttaatc tttgcaggga ttgcagaacg tgagtcgaca atggattccc
      121 tgaaatgtta acattcgtac aaatgggcaa tgtgattata ttttcgggta atgaagttag
      181 taggttgttg gaaacatcca gtgccaatag ttcttgcggt aatctggata atctgcgtag
      241 ttgattttca cctaccttca aatattttaa gctctgagga aattctggta gttcctgtag
      301 tctgttgccg gaaacaaaga gttcttgtag tctatgaggc aattcaggca agacagtaag
      361 gcgattacct tcaatacgta atattgttag cgttgatgga agctctggta acgtctccag
      421 actattaaaa cgtgccgaga gtgattgtaa atatggtggt aatgatggca gacatgacaa
      481 atttcgattg aatgaaatat taagcttaat cagttgagat ggaagtgtcg ggagtatatt
      541 caagttgtta ccatttacat gaagttctgt caaaagcgga gaattttcag gtagggagat
      601 taactcattg ttggaaatat taagttcacg tatatgcaaa gggagtggtg gtaaagaacg
      661 tagtttcagt aagcttaaat tgagaacagg ctcccgggta tccagacata ttttcaataa
      721 tcgaaccgcc tggatgcgat tttctgttgt tgttccttcc ctagcccagt catcccagat
      781 gcggtggtaa tctctggtac actgttcttc taccgataaa cgagatatac atgataagcc
      841 acaaagggaa cgaaaaaaag aatgattgtt gatcggtttc atttattaac ccctcactat
      901 tcatatcttg gtctattaat gcaaataaaa cgagaaagtc tttaaaaaaa caatcctaga
      961 gctttatcgg cgttttcatt ccggctggag gaatatgttc ctgtctttcc agtaaccgtc
     1021 gattgttata tcag
//
LOCUS       DAZPFP010000424         1031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPFP010000424 DAZPFP010000000
VERSION     DAZPFP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.432"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            301..411
                     /locus_tag="ACHUR4_004323"
                     /pseudo
     CDS             301..411
                     /locus_tag="ACHUR4_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            489..803
                     /locus_tag="ACHUR4_004324"
     CDS             489..803
                     /locus_tag="ACHUR4_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457603.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
BASE COUNT          310 a          231 c          222 g          268 t
ORIGIN      
        1 ttgcacttag aaggatctct ccccgaattg tgtgatctgc ttaatttcta aaaagaaaca
       61 aacagttaac tagactgagc gatactaccc atagaaccag tttcccatga cgaacgacac
      121 tggacgcaaa aacaatcata aaaaacacaa taaatgtaat gaacacatcc caccctatta
      181 agtaaagtgc actgtgatag aggaagggaa atacaggaag gtgtggagcc ttgcagattt
      241 ctggcatcat agtgcccaat acggaatgca taaagccctc tcagactgga gactccacaa
      301 atgaaatata cagtgtttaa ttatatcaag tgcgattaca atcgctgacg acggcacagt
      361 tggtgtggag gtctcagtcc tgaacaattt gaaaaccaga acctcactta agcctgtgtt
      421 catattacgt gggtaggatc atacttttat agaagctgtc agcggctacg tcccagccaa
      481 atcacttcat gctccagcgc cagagaggca aggttgggtt tgctcagctg ccggttgatt
      541 ttgttgcgat tgaaccggac agcgtccagg gcgtcggcaa aagagcgaat ctcaccaatc
      601 gttgtttcat tatccgcatt aacgatagct tcaagaaacg ccaaggtttt atcgaattta
      661 tcagcaacag tggctccggg cataccgtga ccgtttatac aaaacggcgt tttcagcgtg
      721 gagttttcat gaatagcctc aacacgaatg tcgtgaagcc agtgatgtat cggctacgat
      781 cctcagctga tgccagacca taggactgac actacgaaca gcaattttaa tgacgtaaat
      841 cttcataact acaactaaca gggcatcatt atgaatgatg ccggttattt taccgctgtt
      901 ggtcccttca tttagggact gccccaagtt ttttcatact ctctttctca gagagtttag
      961 tctctgggat tccagacggt ccatcattga gccaagggag taaagaactc aaagcattta
     1021 cagaattgtt t
//
LOCUS       DAZPFP010000425         1007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPFP010000425 DAZPFP010000000
VERSION     DAZPFP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.433"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>1007
                     /gene="ipaA"
                     /locus_tag="ACHUR4_004325"
     CDS             <1..>1007
                     /gene="ipaA"
                     /locus_tag="ACHUR4_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR1457604.1"
                     /translation="VAVFPELLRKVLNDILEDIKDSHPIQDGLPTPPEDMPDGGPTPG
                     ANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQSPTSQTNDLLSRNG
                     NSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDNSDGIGSDLLTTSS
                     QERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKSSASLSHRVASQIN
                     KFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLISLIGTKSGTQEREL
                     QEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAIYEKAKEVSSALSK
                     V"
BASE COUNT          390 a          224 c          157 g          236 t
ORIGIN      
        1 cagtagccgt gtttcctgag ctgttgagaa aagtccttaa tgatatttta gaagatataa
       61 aagattcgca tcctatccaa gatggcctcc ctacacctcc cgaagatatg ccagatggcg
      121 gaccaacccc cggagccaat gagaaaacat cccaacctgt aattcactat catataaata
      181 atgataatag aacttacgat aatagagttt ttgacaacag agtatatgac aatagctatc
      241 acgagaaccc agaaaatgat gcacagtctc ctacttctca gacaaacgat ctattatccc
      301 gtaacggaaa ctcattacta aatccacaaa gagcactagt tcaaaaagta acttccgttc
      361 taccacactc tatatcagat actgtccaga catttgcaaa taattcagct ttagaaaagg
      421 ctttcaacca tactccagat aattcggatg gaataggttc agacctgtta actacgagta
      481 gtcaagaaag atctgcaaat aactctcttt ctcggggaca caggcctctg aacatacaga
      541 actcttcaac caccccccct ctccacccgg aaggagtgac aagcagtaat gataactcat
      601 cagatacaac taaaagtagc gcttctcttt ctcatagagt agcttcgcaa atcaataaat
      661 tcaactcaaa cactgattca aaagtacttc agactgattt tttatcaaga aatggagaca
      721 catatttaac acgggaaacg atatttgaag cttcaaaaaa agtaacaaac tccctaagta
      781 atcttatatc tctcattgga actaaatcag gaacacaaga acgagagtta caggaaaaat
      841 caaaggacat tacaaaatcc acaacagaac atagaataaa caacaaatta aaagttacag
      901 atgcaaatat aagaaactac gtaacagaaa ccaacgcaga tacaattgat aaaaatcatg
      961 cgatctatga aaaggcaaaa gaagtatcta gcgccctcag caaggta
//
LOCUS       DAZPFP010000426          993 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPFP010000426 DAZPFP010000000
VERSION     DAZPFP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.434"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            4..627
                     /gene="tetR(B)"
                     /locus_tag="ACHUR4_004326"
     CDS             4..627
                     /gene="tetR(B)"
                     /locus_tag="ACHUR4_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1457605.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(605..>993)
                     /locus_tag="ACHUR4_004327"
     CDS             complement(605..>993)
                     /locus_tag="ACHUR4_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1457606.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT          320 a          195 c          208 g          270 t
ORIGIN      
        1 atgatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
       61 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      121 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      181 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      241 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      301 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      361 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
      421 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      481 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      541 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      601 caacttaaat gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg atggtaactt
      661 cacggtaacc aagatgtcga gttaaccacc ctttagattc ataaagcgaa aataatgcgg
      721 ctccaacgta cccacctaaa tggaaacggc gttcactcca atctaaacac gcacaacaga
      781 ttttacgtga atgtttggaa ggaacgtcaa ttcccatttc atgaaaatat tgaataccac
      841 ttaatgtgat cattgaacca ttttcagtga tccattgctg ttgacaaagg gaatcataga
      901 tcttaacggc aacttcgcca gctaaatgat catagcaagt acgtgctttt cgtaaatgca
      961 ctggcgtgga aactttggca tgtacgccat ggt
//
LOCUS       DAZPFP010000427          981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPFP010000427 DAZPFP010000000
VERSION     DAZPFP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.435"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..699)
                     /locus_tag="ACHUR4_004328"
     CDS             complement(<1..699)
                     /locus_tag="ACHUR4_004328"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1457607.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIG"
     gene            complement(696..>981)
                     /locus_tag="ACHUR4_004329"
     CDS             complement(696..>981)
                     /locus_tag="ACHUR4_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR1457608.1"
                     /translation="IYAAHTSQALANFTGFVVAVSFSFFANARFTFKALTTAMRYMLY
                     VGFMGILSVIVGWAADKCSLPPIVTLITFSAISLVCGFVYSKFIVFRDAK"
BASE COUNT          285 a          232 c          200 g          264 t
ORIGIN      
        1 gcctatataa gtccatacgc gaagaggaaa ggttgaaaaa cttgtgatac cttccagtgc
       61 cagattccat aatttccagc cattaaattt tgagatgcca gcaacacgct ctgcgcgtac
      121 atattcaacg acatccgtcg gaccacccac ccagctcagt atgcctttca tgaaaagatt
      181 gcgctcaggc aacagtttaa tgttctccac aacctcacga gacatgagtc gaaaatctcc
      241 gacattttcc tcgatctttg gggtgcttat tttgttgtgt aatttataga accactcagc
      301 tgtcttacgt ttcagtcgtc catcagttga gcggtctgaa cgtttagcaa gcaccatgtc
      361 agcacctgcc tgccactttt caataagatg agggataacc tcaattgggt cttgcaggtc
      421 gacatcaata ggaattacag catcgccgct tgcatggtct aaccctgcaa ataaggctgg
      481 ttctttacca aagttgcgtg taaatgacag cggaacaact agcgggtctg aaacagccag
      541 cgcgttaata attgactctg tggcatcttt acttccgtca tttatgaata caatttctac
      601 ttcatatggc ttcaactctt ggaattcacg taccgtttta tagaaaacag gtatcgcttc
      661 ttcttcattg aagacaggaa cgacaagaga tattttcatt tcgcatccct aaagacaatg
      721 aactttgaat agacgaaacc gcacaccaga ctgatggcgg agaaggtgat aagagtgact
      781 attggaggaa gtgagcattt atcagctgcc caaccaacaa tcacactcaa tattcccata
      841 aatcccacgt ataacatgta gcgcatcgct gtagtcaatg ctttgaatgt gaatcttgca
      901 ttcgcgaaga agctaaagct cacagccact acgaaacctg tgaagtttgc cagagcctga
      961 ctggtatgtg cggcatagat a
//
LOCUS       DAZPFP010000428          977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPFP010000428 DAZPFP010000000
VERSION     DAZPFP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.436"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            101..795
                     /locus_tag="ACHUR4_004330"
     CDS             join(101..347,347..795)
                     /locus_tag="ACHUR4_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1457609.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
BASE COUNT          253 a          225 c          236 g          263 t
ORIGIN      
        1 attataattt agttattgtt agtatgtttt ataatgctgg atgggtgatg cctctaatta
       61 gttgaatctg atgtataata cgggcttttg aggttatctc atgaccagcg ttaatattca
      121 ttgtccccgt tgtcagtcag ctcaggttta ccgccatggt cagaacccta aaggccgtga
      181 cagacttcgc tgccgtgact gccaccgtgt gtttcagttc acttatactt atcaagcacg
      241 taagccgggt atgaaagagc tgataactga aatggccttt aatggtgccg gggttcgcga
      301 taccgccagg acactgaaaa ttggtagtaa caccgtcatc cggactttaa aaaactcgcg
      361 ccaaagcgaa taacttcttc gcctgttgcc catgctgatg tggcgtttat ctgcgagctt
      421 gatgagcaat ggagctacgt tggcagtaaa gcccggcaac attggctcgg gtacgcgtac
      481 aacaccaaaa caggcggtgt actggcctac acttttggtc cccgaaccga tcaaacgtgc
      541 cgggagctac tggcactgct tacacccttc aacatcggca tgctgaccag cgatgactgg
      601 ggcagctatg gccgggaggt gccgaagaat aagcatctga ccggcaaaat attcacccaa
      661 cgcattgagc gtaataatct gacgctacgc acccgcatta agcggttggg tcgtaaaaca
      721 atctgcttct cacgctcagt tgagatccac gaaaaagtga tcggagcgtt tatcgaaaaa
      781 cacatgttct actaattgga tgcactaccc agtatgtaac tgggtttgtc cataaaatgc
      841 accttactca gttggtgtcc aacttttggg gtgcagttca gaaggacggg attttacggt
      901 gtaccggata aatttaaaac tgcctgatac tgaatgtatg aggtttcgta atgctcaatt
      961 cccctagtac cgttttg
//
LOCUS       DAZPFP010000429          970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPFP010000429 DAZPFP010000000
VERSION     DAZPFP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.437"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHUR4_004331"
     CDS             <1..167
                     /locus_tag="ACHUR4_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR1457610.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHUR4_004332"
     CDS             152..790
                     /locus_tag="ACHUR4_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR1457611.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
BASE COUNT          260 a          189 c          287 g          234 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca
//
LOCUS       DAZPFP010000430          966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPFP010000430 DAZPFP010000000
VERSION     DAZPFP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.438"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            257..859
                     /gene="fimB"
                     /locus_tag="ACHUR4_004333"
                     /pseudo
     CDS             257..859
                     /gene="fimB"
                     /locus_tag="ACHUR4_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          290 a          165 c          209 g          302 t
ORIGIN      
        1 tgatgagggt tattattaga ttcgtatcag attgataaat atataaaggt acatagcatg
       61 caagagcatg gcgtttgtat ggcaaagtta ttataagtaa ctatcgctac tccatttaag
      121 ttcgctcaga agaactggtc cacttacgta agttattaag caaacgttcg cttttataaa
      181 cataatcagg ataaaaatgt tggattattg ctaacccagc acagctagtg cgcgtctgta
      241 attataaggg aaaacgatga ggaataaggc tgataacaaa aaaaggaact tccttaccca
      301 tagtgaaatc gagtcactcc ttaaagcagc aaataccggg cctcatgcaa cacgtaatta
      361 ttgtctgatt ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag
      421 gatttcggat attgatctta aagcaaagtg tatatatatc catcgattaa aaaaaggctt
      481 ttcaacaacg cacccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat
      541 ccgtacttct tatccgcatg ctgagagcga gtgggtattt ttatcacgta aggggaatcc
      601 gctttctcgg caacagtttt accatattat ctcgacttct ggtggtaatg ccgggttgtc
      661 actggagatt catccgcaca tgttacgcta ttcgtgtggt tttgctttgg cgaatatggg
      721 aatagatacg cgacttatct aggattatct tgggcatcgc aatattcgtc atactgtctg
      781 gtataccgcc agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca
      841 gcgtcatgct gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta
      901 ttcttacgat atatcaaaaa tgattaacca tttattaatt gatgttattg taagtgtgat
      961 tcttaa
//
LOCUS       DAZPFP010000431          940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPFP010000431 DAZPFP010000000
VERSION     DAZPFP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.439"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            172..351
                     /locus_tag="ACHUR4_004334"
     CDS             172..351
                     /locus_tag="ACHUR4_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1457612.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            437..937
                     /locus_tag="ACHUR4_004335"
     CDS             437..937
                     /locus_tag="ACHUR4_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1457613.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
BASE COUNT          330 a          185 c          208 g          217 t
ORIGIN      
        1 acatattcct caggtaattg ttaatatatc cagaatgttc ctcaaaatat atttccctct
       61 atcttctcgt tgcgcttaat ttgactaatt ctcattagtg actaatttta atgagtgtcg
      121 acacacaaca ctcatattaa tgaaacaatg caacgcaacg ggagaaataa catggccgaa
      181 catcgtggta gttcaggaaa tttcgccgaa gaccgtgaga aggcatccga cgcaggccgt
      241 aaaggcggtc agcatagcgg cggtaatttt aaaaatgatc cgcaacgcgc atctgaagcg
      301 ggtaaaaaag gcggtcaaca aagcggtggt aataaatcag gcaaatcctg attcaccgtt
      361 aattattaat atcagctgat aaagataatt gcatctgcgg gccgtatggc tcgcagtgat
      421 ttcactggag aaaaatatga atatgaagac cattgaagat gtatttattc aactgctttc
      481 agatacctac agcgcagaaa aacaattaac ccgggcactg gcaaaactcg caagagcaac
      541 atcaaatgaa aaattaagtc aggcttttca tgcgcacctc gaggaaactc atggacagat
      601 tgaacgaatt gatcaagttg tggaatcaga atcaaatctg aaaattaagc gcatgaaatg
      661 tgtggcaatg gaaggtctta ttgaagaagc taatgaggtc atcgaaagta ccgagaaaaa
      721 cgaagtgcgt gatgccgcac tgattgccgc agcacagaaa gtcgagcatt atgagattgc
      781 cagttacggg acattagcga cgctggctga acaattaggt taccgtaaag cagcgaagct
      841 tctgaaagaa accctggaag aagaaaaggc caccgacatc aaactgactg atctggccct
      901 taataacgta aataagaaag ccgaaaataa agcctgaaat
//
LOCUS       DAZPFP010000432          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPFP010000432 DAZPFP010000000
VERSION     DAZPFP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.440"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..225
                     /gene="mxiH"
                     /locus_tag="ACHUR4_004336"
     CDS             <1..225
                     /gene="mxiH"
                     /locus_tag="ACHUR4_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR1457614.1"
                     /translation="WTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNPQLLAEYQSK
                     LSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            238..531
                     /gene="mxiI"
                     /locus_tag="ACHUR4_004337"
     CDS             238..531
                     /gene="mxiI"
                     /locus_tag="ACHUR4_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR1457615.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            537..>935
                     /gene="mxiJ"
                     /locus_tag="ACHUR4_004338"
     CDS             537..>935
                     /gene="mxiJ"
                     /locus_tag="ACHUR4_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR1457616.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSY"
BASE COUNT          314 a          144 c          191 g          286 t
ORIGIN      
        1 tggacattga gttcattatc tgaaactttt gatgatggaa ctcaaacatt acaaggtgaa
       61 ctaacattgg cactagataa attagctaaa aatccttcga atccacagtt gctggctgaa
      121 taccaaagta aattatctga atatacatta tataggaacg cgcaatccaa tacagtgaaa
      181 gtgattaagg atgttgatgc tgcaattatt caaaacttca gataataggg agcattcatg
      241 aattacattt atccagtcaa tcaggttgat attatcaaag ccagtgattt tcaatctcaa
      301 gagatatcaa gtctggaaga cgtcgtgtcg gctaaatata gtgatattaa gatggataca
      361 gatattcaag tatcacaaat aatggagatg gtaagcaatc cagaatcatt aaacccagaa
      421 tctttggcca agttacagac gacgctctca aattattcaa taggagtatc attagctggc
      481 acgttagcaa gaaaaacagt ttcggctgtt gaaactttat taaagtctta atttatatga
      541 ttaggtataa aggttttatt ttattcttgt tgctgatgtt gattggatgt gagcaacgtg
      601 aagagttaat ttctaattta tctcaaagac aggcaaatga aataatatct gtgctagaac
      661 gccataatat tactgctaga aaagttgatg gaggtaaaca ggggatctcg gtacaagtcg
      721 aaaaggggac atttgcatcg gcagttgatt tgatgcgcat gtacgatttg ccaaatccgg
      781 agagagttga tatctcacaa atgtttccta cagattcatt agtgtcttct ccaagagctg
      841 aaaaggcccg tttatatagt gctattgagc aacggctgga acagtcttta gtttctattg
      901 gtggtgttat ttcggcaaaa atacatgtta gctat
//
LOCUS       DAZPFP010000433          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPFP010000433 DAZPFP010000000
VERSION     DAZPFP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.441"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHUR4_004339"
     CDS             complement(55..243)
                     /locus_tag="ACHUR4_004339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457617.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHUR4_004340"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHUR4_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAZPFP010000434          930 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPFP010000434 DAZPFP010000000
VERSION     DAZPFP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.442"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            217..>930
                     /locus_tag="ACHUR4_004341"
     CDS             217..>930
                     /locus_tag="ACHUR4_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1457618.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVIT"
BASE COUNT          295 a          162 c          209 g          264 t
ORIGIN      
        1 ggtgattttt tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt
       61 gaataaaaga aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat
      121 aatctaaaaa agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac
      181 gcggatttat cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc
      241 aaagtcgtat tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat
      301 gtcaaagtga ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc
      361 tatgaacaag ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg
      421 cttgtgcaga gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact
      481 ccgccactgt tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg
      541 ccagctttac aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa
      601 aagagtgatg tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac
      661 atccaggtta acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc
      721 aagatggtta atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg
      781 gtcaatcagt cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt
      841 gagaaactag attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc
      901 ggccaggata ttaaatggtc tgtgattact
//
LOCUS       DAZPFP010000435          926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPFP010000435 DAZPFP010000000
VERSION     DAZPFP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.443"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..250)
                     /locus_tag="ACHUR4_004342"
                     /pseudo
     CDS             complement(1..250)
                     /locus_tag="ACHUR4_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(298..483)
                     /locus_tag="ACHUR4_004343"
     CDS             complement(298..483)
                     /locus_tag="ACHUR4_004343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457619.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            646..>926
                     /locus_tag="ACHUR4_004344"
     CDS             646..>926
                     /locus_tag="ACHUR4_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1457620.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYESASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT          223 a          279 c          202 g          222 t
ORIGIN      
        1 cactgtctcc gttatggcgt acaccacatc ttccagtaat cgccgcttca ccggactcag
       61 gaccccgtat caccatccgg aaccccttat ccgccacatg ccaggagaac ctttccataa
      121 actcgtcgcc actcatcaca aggttttctt ctcgttttgt ccggtggctg ttgtaacgca
      181 ggccaatttc atcctgaccg gcataatgtt ccaggcggct catctgcacc ggctgcttct
      241 ccagccagtt aagccatcgg tttccctgct gaaaaatatc ggcaaaacgg ggaagcatca
      301 gaaaggcgga ataacgccat ccggccagtg aaccgtaccg cattcagggc aatacatgcc
      361 gtcggcgttg ataccggcag gaattgtcgc aaattcccgc tccatgcagc gggcaatttc
      421 cggataccct tcgtcatcaa cacgtacaaa ccagaagacc agttttttgt ttccggcatc
      481 cacaaagaac ggaatattca ggtctgcgca acactcaacg gcatcgtcaa aattatcaaa
      541 gcgcagaacc tcagcttctt cgtaaccggc tcatttaaac cgtctggtct gtttcctccg
      601 gctccacaaa aataatgtcc atcattttga agtggacact atcgtatgga ataccggacc
      661 tggatcactg aagctttacg ccttcatttc gaagaacatt taccacgggt tgtagccgga
      721 cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt tcgtgcgctt ccgcaacgct
      781 ggcctttcat ggcctctgcc cgcaggtatg tcggagcagg aacttgatgc ccttctttac
      841 gaaagtgcct ccacagtacc tgtcgtactt actgaaagca ccgttatgcc caaactcccg
      901 gtggtaaaaa aacgtccccg gcggcc
//
LOCUS       DAZPFP010000436          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPFP010000436 DAZPFP010000000
VERSION     DAZPFP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.444"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHUR4_004345"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHUR4_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHUR4_004346"
     CDS             300..>923
                     /locus_tag="ACHUR4_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1457621.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZPFP010000437          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPFP010000437 DAZPFP010000000
VERSION     DAZPFP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.445"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            91..>923
                     /gene="ipaD"
                     /locus_tag="ACHUR4_004347"
     CDS             91..>923
                     /gene="ipaD"
                     /locus_tag="ACHUR4_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR1457622.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QA"
BASE COUNT          347 a          181 c          160 g          235 t
ORIGIN      
        1 ttcgacagcc agtcagattg ctggtaacat tcgagcttaa tatatccaag agccataata
       61 atatatggct cttcctgtaa ggaaataacc atgaatataa caactctgac taatagtatt
      121 tccacctcat cattcagtcc aaacaatacc aacggttcat caaccgaaac agttaattct
      181 gatataaaaa caacgaccag ttctcatcct gtaagttccc ttactatgct caacgacacc
      241 cttcataata tcagaacaac aaatcaggca ttaaagaaag agctttcaca aaaaacgttg
      301 actaaaacat cgctagaaga aatagcatta cattcatctc agattagcat ggatgtaaat
      361 aaatccgctc aactattaga tattctttcc aggcacgaat atccaattaa taaagacgca
      421 agagaattat tacattcagc tccgaaagaa gccgagcttg atggagatca aatgatatct
      481 catagagaac tgtgggctaa aattgcaaac tccatcaatg atattaatga acagtatctg
      541 aaagtatatg aacatgccgt tagttcatat actcaaatgt atcaagattt tagcgctgtt
      601 ctttccagtc ttgccggctg gatctctccc ggaggtaacg acggaaactc cgtgaaatta
      661 caagtcaact cgcttaaaaa ggcattggaa gaactcaagg aaaaatataa agataaaccg
      721 ctatatccag caaataatac tgttagtcag gaacaagcaa ataaatggct tacagaatta
      781 ggtggaacaa tcggcaaggt atctcaaaaa aacgggggat atgttgtcag tataaacatg
      841 accccaatag acaatatgtt aaaaagctta gataatctag gtggaaatgg cgaggttgtg
      901 ctagataatg caaaatatca ggc
//
LOCUS       DAZPFP010000438          917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPFP010000438 DAZPFP010000000
VERSION     DAZPFP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.446"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>917)
                     /gene="mxiA"
                     /locus_tag="ACHUR4_004348"
                     /gene_synonym="virH"
     CDS             complement(<1..>917)
                     /gene="mxiA"
                     /locus_tag="ACHUR4_004348"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR1457623.1"
                     /translation="LNIVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTS
                     RLILVDADAGKIITTFGQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFS
                     LDGMPGKQMSIDADLKAGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGII
                     IIFVNLIGGISVGMSQHGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVN
                     GDSDNMGRNIMSQIFGNPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKV
                     VEKEKSLSESDSSGYTGTFDIDNSHDSSL"
BASE COUNT          342 a          204 c          135 g          236 t
ORIGIN      
        1 ccaaagatga gtcatggcta ttatcaatat caaatgtacc agtatagcca ctagaatcag
       61 actcagacag acttttttct ttttctacga cctttttata atagaataaa gccgtcaaag
      121 taactgctat caggaaaaaa acaaaaaacg gaaaacctgg caacatccct atcgccaagg
      181 caagagctga tgtaacaatg agaacaaaag gattcccaaa tatctgggac attatgttac
      241 gtcccatatt atcgctatca ccattaactc ttgtaacgat aaatcctgca cttatagaaa
      301 ttaacagagc aggaatctga gagaccaatc cgtcaccaat agttagtatg gtataagtag
      361 ataatgcacc agatagggac attccatgtt gactcatacc aacagaaata cctcctatta
      421 aattgacaaa aataataatg atgccagcaa ttgcatcacc tttaatgaac ttcattgctc
      481 catcaaatga accatacaat tgactctcac gttccagaat actgcgtctt tctttagctc
      541 ctgcggcatc aataattcca gcctttaaat ccgcatcaat gctcatttgt ttccccggca
      601 taccgtcaag agaaaagcga gcagctacct ccgcaaccct ttcagatcct tttgttataa
      661 caatgaactg cacaacagtt actattgaaa aaataacaaa acctacagcc agactatcac
      721 caatgacaaa ctggccaaat gtagtaataa ttttacccgc atccgcatca actaaaatta
      781 gtcgactagt acttattgat aaagcgagac gaaataatgt cgttatcaac aaaaccgaag
      841 ggaatgtaga aaaactcaat attctttcaa tataaaatga tcccataaaa acgagaatag
      901 caagtacaat attaagc
//
LOCUS       DAZPFP010000439          891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPFP010000439 DAZPFP010000000
VERSION     DAZPFP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.447"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..762
                     /gene="lpxM"
                     /locus_tag="ACHUR4_004349"
                     /gene_synonym="msbB"
     CDS             <1..762
                     /gene="lpxM"
                     /locus_tag="ACHUR4_004349"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1457624.1"
                     /translation="RRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELAIRG
                     PEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQR
                     NPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFF
                     ATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQ
                     MNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          257 a          176 c          213 g          245 t
ORIGIN      
        1 cgcagggcga cgattaattt atcgctttgt ttcccggaaa agagtgatac agaacgggaa
       61 ataattgtcg acaatatgtt tgccacagca ttgcaatcca tagtgatgat ggcagaactg
      121 gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct ggaaaggcct cgaaattctt
      181 gaggaaattc ggcacaataa cagaaatgtg atttttctgg ttccccatgg ctggagcgtg
      241 gatattcctg caatgttgct ggcagctcag ggggaaaaaa tggccgccat gtttcatcag
      301 caacgaaatc cagtgattga ttatgtctgg aattctgtac ggcgtaaatt cggggggcgc
      361 ttacattccc gggaggatgg gataaaacca tttattcagt cagtacgcca gggatactgg
      421 gggtattacc ttccagatca ggatcatggt cctgaataca gtgaatttgc tgattttttt
      481 gcgacctata aagcgacatt accaattatt ggacgtctga tgaacatcag tcaggctatg
      541 attataccgc ttttcccggt ttatgatgaa aaaaaacatt tcctgactat tgaagttcgg
      601 ccaccaatgg atgcatgcat tgccagcgcg gacaataaaa tgattgcccg acaaatgaac
      661 aaaacagtgg aaattttggt ggggtcacat ccggaacagt atatctgggt tttaaaattg
      721 ttaaaaacgc gcaaatcaaa cgaagcggac ccgtaccctt gaaattataa agtacaggta
      781 tttccactag ttgtttctta caggttacca atcgaaacac atcccccttc cgtgtgttgc
      841 ccgtttggca cggaggactg tgtagatgca gttcgttaaa tttaatttat g
//
LOCUS       DAZPFP010000440          886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPFP010000440 DAZPFP010000000
VERSION     DAZPFP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.448"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>886)
                     /locus_tag="ACHUR4_004350"
     CDS             complement(<1..>886)
                     /locus_tag="ACHUR4_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1457625.1"
                     /translation="FVRTLSACTLNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKR
                     TVFTCDSHFELSQLSAKDFFDDFYWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELI
                     NSRVIYHAITKNLTEVLFILMEKYKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYS
                     DIIQVYGKILNMCNLSQETILTLLAAVGANNVSGLCMSFMNGHVDTIKAYGEIVFKTP
                     LTSDKRLYLLAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKH
                     GLFMALQNGHTKAIMAYG"
BASE COUNT          268 a          177 c          141 g          300 t
ORIGIN      
        1 tctccatacg ccataatggc ctttgtgtgc ccattttgaa gtgccataaa caacccatgc
       61 tttactttca ataattcttt aatttgttca ctactaagca ttttcttatt aagcagactt
      121 ccgaacatac gaatactatc tgcatgtcca ttctgcaatg caaaaaataa cccaggtaaa
      181 tcatggctgt ctttcgcagc taatagatat aatctcttat cggaagttaa cggggtttta
      241 aagactattt ccccataggc ttttattgtg tctacatgtc cgttcataaa agacatgcat
      301 aaacccgaca cattgtttgc ccctaccgca gcaagcaatg taagtattgt ttcttgacta
      361 agattgcaca tatttaatat cttaccatat acttgaataa tgtctgaata tccgttttga
      421 agtgctatat aaaatgcagg agtaccatca gagcttctcg tggccagaag gttcaccagt
      481 ttagattgag aaatttcacc atttttgtat ttttccatca gtataaataa tacttctgtc
      541 agattctttg ttatcgcatg atatataact ctggaattga tcaattcatc tggtaaaagt
      601 ttcactgtgt ttgttagagg ggaattatgc ctatcttcaa atattacctg cccgggttgc
      661 tccagaccat aaattttcca ataaaaatca tcaaaaaaat ccttagcgga taattgactc
      721 agttcgaaat ggctatcaca agtaaataca gtacgtttat gagtaacagt tcgattagga
      781 tcgaaaaact gaacaaccca tttttcactt tcttgtacgc gctttatttt caaccctaac
      841 gccatttggt ggtttaatgt gcatgctgat aatgttctga caaagt
//
LOCUS       DAZPFP010000441          874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPFP010000441 DAZPFP010000000
VERSION     DAZPFP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.449"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..132
                     /gene="yjbL"
                     /locus_tag="ACHUR4_004351"
     CDS             <1..132
                     /gene="yjbL"
                     /locus_tag="ACHUR4_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1457626.1"
                     /translation="NNIYGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            156..863
                     /locus_tag="ACHUR4_004352"
     CDS             156..863
                     /locus_tag="ACHUR4_004352"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1457627.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
BASE COUNT          290 a          132 c          177 g          275 t
ORIGIN      
        1 aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga tataattatt
       61 gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc acgtaaaaaa
      121 atgtgttttt aattgagcac ataaatgatc tcaaaatgtg ggtcaataag tatactgacg
      181 attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt gaccgggcta
      241 tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat gcaatgccca
      301 ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg gttcagctat
      361 tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa gcaataggtt
      421 ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc ttaaaattat
      481 ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta tcagaaacag
      541 tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa ttatatcaac
      601 gtgatatgaa gaagtacctg ggagagatga agcgtctggc agatggggga gaaatgaatt
      661 ttgatggcta tctggataag atgtcacatc tggtgaatga aggaacgctc gatcctgata
      721 ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag tcgtttgacc
      781 caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt tatgacttgt
      841 tcggggttaa atcggagaaa taatatgtga agtt
//
LOCUS       DAZPFP010000442          871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPFP010000442 DAZPFP010000000
VERSION     DAZPFP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.450"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..758
                     /gene="lpxM"
                     /locus_tag="ACHUR4_004353"
                     /gene_synonym="msbB"
     CDS             <1..758
                     /gene="lpxM"
                     /locus_tag="ACHUR4_004353"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1457628.1"
                     /translation="ATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELAIRGPE
                     KFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNP
                     VIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFAT
                     YKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMN
                     KTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          270 a          170 c          205 g          226 t
ORIGIN      
        1 gggcgacgat taatttatcg ctttgtttcc cagaaaagag tgatacagaa cgggaaataa
       61 ttgtcgacaa aatgtttgtc acagcattgc aatccatagt gatgatggca gaactggcaa
      121 ttcgtggtcc ggaaaagttc cagaaacgag tgttctggaa aggcctcgaa attcttgagg
      181 aaattcgaca caataataga aatgtgattt ttctggttcc ccatggctgg agcgtggata
      241 ttcctgcaat gttgctggca gctcagggaa aaaaaatggc cgccatgttt catcagcaac
      301 gaaatccagt gattgattat gtctggaatt cagtacggcg taaattcggg gggcgcttac
      361 atgcccggga ggatgggata aaaccattta ttcagtcagt acgccaggga tactggggat
      421 attacctccc agatcaggat catggtcctg aatacagtga atttgctgat ttttttgcga
      481 cctataaagc gacattacca atcattggac gtctgatgaa catcagtcag gctatgatta
      541 taccactttt cccggtttat gatgaaaaaa aacatctact gactattgaa attcggccac
      601 caatggatgc ttgcattgcc agcgcggaca ataaaacgat tgcccgacaa atgaacaaaa
      661 cagtggaaat tttggtgggg ccacatccgg aacagtatgt ctgggtttta aaattgttaa
      721 aaacacgcaa atcaaacgaa gcggacccgt acccttgaaa ttataaagta caagtatttc
      781 cactagttgt ttgtaagcgt acagcctgaa ccgtctggtc agaatctgac gaattagaca
      841 aagtggtgtc caccaaataa gtagtgggaa c
//
LOCUS       DAZPFP010000443          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPFP010000443 DAZPFP010000000
VERSION     DAZPFP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.451"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHUR4_004354"
     CDS             <1..>870
                     /locus_tag="ACHUR4_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1457629.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZPFP010000444          868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPFP010000444 DAZPFP010000000
VERSION     DAZPFP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.452"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(215..697)
                     /gene="sseA"
                     /locus_tag="ACHUR4_004355"
                     /pseudo
     CDS             complement(215..697)
                     /gene="sseA"
                     /locus_tag="ACHUR4_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          204 a          240 c          209 g          215 t
ORIGIN      
        1 gaacacggcg gaaagggccg gtgtcgataa tacgctaaat aaacaataat tactctcttt
       61 tgcttgacaa aaagagagtt actggtgagt attgttttgc tgtgaagcac ctgcgttgct
      121 catgtaacac aagtcaacct gctggaaagg ccgcgaacca gaccagcaat aaaaaaacgc
      181 caaattcggc ggttttttat tgcctgtaat atacttattt cactggctca accggtaaat
      241 ctgcccgcgc gccccattca ctccatgcgc cgtcgtacag tttcacgttt ggcacatcca
      301 gcgtcgcgag tgctaacaaa accacggctg ccgttacacc agagccgcag ctgacgataa
      361 tcggtttgtc atagctgacg ccgcgaccaa aaaatatcgc atccagttca tcggtcgttt
      421 ttagttcgcc ttcgcgcacc agttccgtcc acggaacgtt caatgcaccg ggaatatgtc
      481 cgcgacgtaa acctgggcga ggttcatcaa cttctgcgtt aaaacgtgca gccgggcggg
      541 catcaataat ttgcgccgta ttttcatggc ttgccaataa cacatcggtt actttcacca
      601 ctgcttcagg attaaacgcg gcgttaaact ctccttccgg cagctctact gcaccttctt
      661 ctaatagcag gtcatcgcgc tgccagcctg caagtccccg ttactttatt cgtacccctt
      721 ataatggggt gttagccagc cagacccggc atgattactg cccccagtcg tccatgatcc
      781 ggggggggat gtcaccgggt ctggtggggc gctggtaacc gctaataggg gtcaggtcag
      841 gcacttttgc cgggaccgtc tgtaacgt
//
LOCUS       DAZPFP010000445          868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPFP010000445 DAZPFP010000000
VERSION     DAZPFP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.453"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..503
                     /gene="virA"
                     /locus_tag="ACHUR4_004356"
     CDS             <1..503
                     /gene="virA"
                     /locus_tag="ACHUR4_004356"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1457630.1"
                     /translation="SYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTSAF
                     WQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAISA
                     SGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVSFA
                     IISPDV"
     gene            682..>868
                     /locus_tag="ACHUR4_004357"
     CDS             682..>868
                     /locus_tag="ACHUR4_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457631.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          277 a          197 c          163 g          231 t
ORIGIN      
        1 taagttacaa ccaagtaaat aaaataatca attcaaactt cccccaacaa gacctatgtt
       61 ttcaaacaga aaaacttttg tttacttcac tctttcaaga ccctgcattt atatctgcac
      121 taacatctgc attctggcaa tctcttcaca tcacgtcttc ctctgtggaa cacatatatg
      181 cccaaattat gtccgaaaac atagaaaaca ggcttaattt catgcctgaa caacgagtta
      241 ttaacaattg tggacatatt ataaaaatca acgcagttgt ccctaaaaac gacactgcaa
      301 tctctgcctc tggggggagg gcttacgaag ttagctcatc aatattacca tcgcacataa
      361 catgtaatgg tgttggtatt aataaaatag aaacaagcta tctcgttcat gctggaacac
      421 ttccaagcag tgaagggctt cgcaatgcta tccctccaga aagtcgtcaa gtatcatttg
      481 ccatcatatc tcctgatgtt taagccaata ttatattggc tgccagactg taaaaaaacg
      541 ttcttcagaa cagctctata aactattaaa ccaaagctaa aaggatgggg accaatagaa
      601 tctcagccca tcctttttat atcattatga accgccccgg aaatcctgga gactaaactc
      661 cctgagaaag aggtaaacag gatgactaaa aatacacgtt tttcccctga agtccgtcaa
      721 cgggcagttc gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcagcgatt
      781 tgttccattg ccccaaagat tggctgtata ctggaaactc tgcgtgtctg ggtacgccag
      841 catgagcggg ataccggggg cggtgagg
//
LOCUS       DAZPFP010000446          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPFP010000446 DAZPFP010000000
VERSION     DAZPFP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.454"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>852)
                     /locus_tag="ACHUR4_004358"
     CDS             complement(<1..>852)
                     /locus_tag="ACHUR4_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1457632.1"
                     /translation="ARTEATNDLYFSRVSQWDDWLSNYTTLQYRGQFDVVRPEVRKLV
                     AEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQIESGVGAW
                     RLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSKQMDKSDAKHCTVINALSRNGW
                     KEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEEKEKVFIYRDPLTG
                     EPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTQILKDREKIAGEHIP
                     IVPVYGEW"
BASE COUNT          179 a          237 c          207 g          229 t
ORIGIN      
        1 ccattcgcca tacactggaa cgattggaat atgctctccg gctatcttct cgcggtcttt
       61 cagtatctgc gtacaggtga tgatcgactt atacacacgc cgacgcttca ccttgcgctc
      121 tgctacctta atgaatccac gattagccag gtcgtcgatg acgtctttga tatcctgctg
      181 gtaatagctg accggctcac ctgtcagcgg gtcgcggtag atgaagacct tctccttctc
      241 ctcttctacc tcgtaatact cagcgacgta gacgacatca ttcgacaccc acgggaatag
      301 ccatgtatcg ttaggattct ggaaagatgg cagggtatcc gggtcaatac cgtaatcctc
      361 tgcgaactct ttccagccat tgcgcgacaa ggcgttaatc accgtgcagt gcttagcgtc
      421 gctcttatcc atctgcttgc tgttggcgtc ccatatgacg tgtgagcagg cttcatggat
      481 tggcaggcgt cggattacct gattgttgct tgttggatcg ttgtcttcgt actgcgtgac
      541 cagacgccat gcaccaacgc ctgactctat ctgctcacga acgccaacgt taacggcaat
      601 ctttgccgtg ttatggcgca tatcagtacg atacatcccc atcaatacat cggctgcatc
      661 aggattagcg ccgtctttgg gtcggaagag aacgtcgaca gggttccggc gcatctctgc
      721 gaccagtttc ctgacctccg ggcgaacaac atcgaattgt ccgcgatatt gcagggtagt
      781 gtagtttgat agccagtcat cccattgcga cactcggcta aaatacaggt catttgtcgc
      841 ctcggttctg gc
//
LOCUS       DAZPFP010000447          832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPFP010000447 DAZPFP010000000
VERSION     DAZPFP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.455"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            48..>832
                     /gene="icsP"
                     /locus_tag="ACHUR4_004359"
     CDS             48..>832
                     /gene="icsP"
                     /locus_tag="ACHUR4_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR1457633.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTP"
BASE COUNT          281 a          147 c          179 g          225 t
ORIGIN      
        1 aaagtaagaa gatcatggac atttcaacca aaaaagtaga gttctcgatg aaattaaaat
       61 tctttgtact tgcactttgt gtacctgcga tctttactac acatgctacc actaactatc
      121 cacttttcat accggacaac atcagtacgg atattagtct aggatctctg agtggcaaaa
      181 caaaagaacg cgtttatcat cccaaggaag gggggagaaa aattagtcaa ctggactgga
      241 aatacagtaa tgcgactatt gttagaggtg gcatcgattg gaagctaatt ccaaaagtgt
      301 ctttcggagt ttccggttgg actactttag gtaaccagaa agcaagcatg gttgataaag
      361 actggaacaa ttccaatacc cctcaggtat ggaccgacca gagctggcat cccaatacgc
      421 atctccgtga tgctaacgaa ttcgagctga atcttaaagg ttggttatta aataatttgg
      481 attatcgact tggactaata gcaggttacc aagagagtcg ttacagtttt aatgcaatgg
      541 gagggagtta tatttatagt gaaaacgggg ggagcaggaa taaaaaaggg gcacatccta
      601 gtggtgaaag aacaataggt tacaaacagc tcttcaaaat accttatatt ggattaactg
      661 ctaattaccg ccatgagaat tttgagtttg gagcagaact gaaatatagt ggttgggttc
      721 tttcatctga tacagataaa cactatcaga ctgagacaat ttttaaagat gaaataaaaa
      781 accaaaatta ctgctctgtt gctgcgaata ttggatacta tgtcaccccc ag
//
LOCUS       DAZPFP010000448          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPFP010000448 DAZPFP010000000
VERSION     DAZPFP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.456"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUR4_004360"
     CDS             <1..326
                     /locus_tag="ACHUR4_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1457634.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUR4_004361"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUR4_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1457635.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZPFP010000449          827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPFP010000449 DAZPFP010000000
VERSION     DAZPFP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.457"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(128..529)
                     /locus_tag="ACHUR4_004362"
                     /pseudo
     CDS             complement(128..529)
                     /locus_tag="ACHUR4_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          205 a          199 c          181 g          242 t
ORIGIN      
        1 gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc
       61 cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg
      121 ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca
      181 acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg
      241 ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc atgaaccgtt
      301 ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact
      361 ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat
      421 tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc accgttgctt
      481 tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa tatggaacgt
      541 aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc aatatcaaca
      601 ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt ataggatatt
      661 atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag atattgccta
      721 agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt agcttgttgc
      781 acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaat
//
LOCUS       DAZPFP010000450          826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPFP010000450 DAZPFP010000000
VERSION     DAZPFP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.458"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            75..>826
                     /gene="ipaH3"
                     /locus_tag="ACHUR4_004363"
     CDS             75..>826
                     /gene="ipaH3"
                     /locus_tag="ACHUR4_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1457636.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          247 a          211 c          134 g          234 t
ORIGIN      
        1 ttcatggaat gaacttcata aaaactccta cttatttctt ttaacaaagc catttgtcca
       61 tcggctttaa ctgaatgtcc atcatgttac cgataaataa taacttttca ttgtcccaaa
      121 attcttttta taacactatt tccggtacat atgctgatta cttttcagca tgggataaat
      181 gggaaaaaca agcgctcccc ggtgaaaatc ggaatgaagc ggtctcccta cttaaagaat
      241 gtctcatcaa tcagttcagt gagcttcaac tgaatcgttt aaatctgtcc tcgctacctg
      301 acaacttacc acctcaaatc actgttctgg aaattactca gaatgcccta atatcattac
      361 cagaattgcc agcatcgctg gaataccttg acgcctgtga caatcgcctg tcaacacttc
      421 ctgaattacc cgcatctctg aaacatcttg atgtagataa caaccaacta accatgcttc
      481 ctgaattgcc tgcattgctg gaatatatta atgcagataa caatcagcta accatgcttc
      541 ctgaattacc tacatcgctg gaagtgctct cagtaagaaa taaccagctg acatttctcc
      601 ctgagttacc tgaatcactg gaagcgctcg atgtaagtac taatcttctg gaaagcctac
      661 cagccgtacc tgtaagaaat catcactcag aggaaaccga gatatttttc cggtgccgcg
      721 agaatcgcat cacacacatt ccggaaaata tacttagcct tgatccgacc tgcactatca
      781 tcctcgaaga caatcctctg tcctcacgga tcagggagtc tctgtc
//
LOCUS       DAZPFP010000451          822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPFP010000451 DAZPFP010000000
VERSION     DAZPFP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.459"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUR4_004364"
     CDS             complement(<1..815)
                     /locus_tag="ACHUR4_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457637.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
BASE COUNT          224 a          135 c          235 g          228 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt at
//
LOCUS       DAZPFP010000452          821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPFP010000452 DAZPFP010000000
VERSION     DAZPFP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.460"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..204
                     /locus_tag="ACHUR4_004365"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACHUR4_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(227..>821)
                     /locus_tag="ACHUR4_004366"
     CDS             complement(227..>821)
                     /locus_tag="ACHUR4_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR1457638.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          187 a          203 c          200 g          231 t
ORIGIN      
        1 acagcgcgga caatgaacag taacggtagt catggaaaac ctcaaatacc agtagattac
       61 cagaaacttc tatcgattga agacattacc ttttgccgca ataggtgggg taacattggt
      121 gaaaacgcat tacgccgggt ggcagttgga aaaaaattat cttttttcgg ctctgatcgt
      181 ggtggagaaa atgcggcaat aatctaaata gctgcgctta ataccgctac acttttgcca
      241 gcccatgttt gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca
      301 acagttgcca cattgagcgg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc
      361 atagccgttc aacatgattc acccatggcg agtaaaccgg ctgataaatg cccctgaact
      421 tcgggttctc cttcagccag ctctgtgttt cccggctttt gtggataatg tagttgtcca
      481 cgatcagcgt gatggttttc gcacgacggt atgtcgcttt aagccgcttc agcaggctga
      541 tgaacagcgc cgaacttttg ctgttgccgc ccacatagct gactttacct gtcccgctgt
      601 gcagcgctcc ggccagataa tatttttcat tctgtcccgg cgtgaccacc cgtttttgct
      661 gtccgcgcag ttgccagtcc gcaccgattt tgggatgaag atggatatcc acttcatctt
      721 cataaaagac cggatgctct gcgctgcatt cgtccagtgc tttatggatt gctgccatct
      781 tttcatcttt atgcgggtca cggatacgca gagttggcgc a
//
LOCUS       DAZPFP010000453          817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPFP010000453 DAZPFP010000000
VERSION     DAZPFP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.461"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..308
                     /locus_tag="ACHUR4_004367"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHUR4_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..742
                     /locus_tag="ACHUR4_004368"
                     /pseudo
     CDS             341..742
                     /locus_tag="ACHUR4_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          233 a          183 c          234 g          167 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccc ggtttaatct
      601 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      661 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      721 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatga
//
LOCUS       DAZPFP010000454          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPFP010000454 DAZPFP010000000
VERSION     DAZPFP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.462"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHUR4_004369"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHUR4_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          235 a          168 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcataga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZPFP010000455          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPFP010000455 DAZPFP010000000
VERSION     DAZPFP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.463"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUR4_004370"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUR4_004370"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1457639.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          195 a          207 c          175 g          212 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaa
//
LOCUS       DAZPFP010000456          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPFP010000456 DAZPFP010000000
VERSION     DAZPFP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.464"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHUR4_004371"
     CDS             complement(<1..666)
                     /locus_tag="ACHUR4_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR1457640.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHUR4_004372"
     CDS             complement(656..>782)
                     /locus_tag="ACHUR4_004372"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR1457641.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZPFP010000457          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPFP010000457 DAZPFP010000000
VERSION     DAZPFP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.465"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(570..>782)
                     /locus_tag="ACHUR4_004373"
     CDS             complement(570..>782)
                     /locus_tag="ACHUR4_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1457642.1"
                     /translation="WLSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
BASE COUNT          213 a          131 c          201 g          237 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 ca
//
LOCUS       DAZPFP010000458          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPFP010000458 DAZPFP010000000
VERSION     DAZPFP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.466"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>775
                     /locus_tag="ACHUR4_004374"
     CDS             <1..>775
                     /locus_tag="ACHUR4_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1457643.1"
                     /translation="CASTRESDHIANMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNS
                     SERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSLTGGEESML
                     KDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRN
                     VDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLANQIKEIVTKKLQK
                     NDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQDA"
BASE COUNT          263 a          101 c          199 g          212 t
ORIGIN      
        1 ttgtgcttct acgagagaga gtgatcatat cgcgaatatg tggctttcaa aagtggtgga
       61 tgatgagggg aaggaaattt tttcgggtat cagacatgga gttatatcag cctatggatt
      121 gaaaaaaaac tcatctgaaa gagctgttgc tgctcgtaat aaagctgagg agttagtaag
      181 cgcagcatta tatagcagac cagagttatt atcacaggct ttgtctggta aaacagtaga
      241 tttaaagatt gtttcgactt ctcttctgac gccgaccagt ttaaccgggg gagaggagag
      301 tatgttaaaa gatcaggtga acgcattaaa agggttaaac tctaaaaggg ggaagccaac
      361 gaaattatta attcggaata gtgatggtct tctgaaagaa gtaagcgtta atctaaaagt
      421 ggtgacattt aactttggtg taaatgaatt agcgctaaaa atgggcttag gctggaggaa
      481 tgttgacaag cttaatgatg aatcaatatg ttctttgttg ggggataatt tcctcaaaaa
      541 tggtgtgatt ggaggttggg ctgcagaagc aatagaaaaa aatccaccat gtaagaatga
      601 tgtgatatat ctggctaacc agataaaaga gattgtaacc aaaaagttac aaaaaaatga
      661 taatggagag ccgtataaat tgtcacaaag aatgactcta ttggcttata ctataggtgc
      721 tgtaccttgc tggaattgta agagtgggaa ggacagaaca ggtatgcagg atgct
//
LOCUS       DAZPFP010000459          763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPFP010000459 DAZPFP010000000
VERSION     DAZPFP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.467"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..637
                     /locus_tag="ACHUR4_004375"
     CDS             <1..637
                     /locus_tag="ACHUR4_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1457644.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          249 a          157 c          186 g          171 t
ORIGIN      
        1 cccgttatct aaaacagaat tcaatgcatt aatggaaagt gaaaaaggac agagccaaaa
       61 cttgtggaaa tttgccgttt actccgggct tcgtcacggg gaactggcag ctctggcgtg
      121 ggaggatgtg gatttcgaga agggaattgt gaatgtcaga agaaacctga cgatacttga
      181 tatgttcggt cccccaaaaa caaatgccgg gatccggacg gtagcattac tgcagccggc
      241 tcttgaagca ctgaaggagc aatacaaact gaccgggcat catcgcaaaa gcgaaatcac
      301 tttttatcat cgggagtacg gcagaactga aaaacaaaaa ctgcattttg ttttcatgcc
      361 cagggtgtgt aacgaaaaac agaaacctta ttactcggta agcagtttgg gtacaagatg
      421 gaatgcagca gtaaaacgtg ctggtattcg ccgccgtaat ccgtaccata cgcgacatac
      481 ttttgcctgc tggctgttga cggcaggagc gaacccggca tttatagcca gccagatggg
      541 gcatgaaact gcgcaaatgg tgtatgaaat ttacggtatg tggattgatg acatgaacga
      601 cgaacagata gccatgttga atgcgcggtt atcgtagttg caaagtttgc ccccaatttg
      661 ccccatttag taccagagaa ctgaaataat gcaagaaaat caacaaatta caaagaaaga
      721 acaatacaac ctgaacaagt aagggcaaaa atcacaacta tct
//
LOCUS       DAZPFP010000460          760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPFP010000460 DAZPFP010000000
VERSION     DAZPFP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.468"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            65..426
                     /locus_tag="ACHUR4_004376"
                     /pseudo
     CDS             65..426
                     /locus_tag="ACHUR4_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            513..>760
                     /locus_tag="ACHUR4_004377"
     CDS             513..>760
                     /locus_tag="ACHUR4_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457645.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          198 a          199 c          180 g          183 t
ORIGIN      
        1 taaaaatgac aatgcctcca attagttgaa ttaggggtta taatacccgc ttttaggttc
       61 tttcatggtc acagttaact ttcattgccc ccgttgccag tccgttcagg tttaccgtca
      121 cgggcagaac ccaaaaggac atgatcgatt ccgttgccgt gactgtcacc gggtgtttca
      181 gctcacttac tgttacgagg cccgtaaacc gggagttaaa aaccaaatca ctgaaatggc
      241 tttcaacggt gccggagttc gcgatactgg ccgtacgctg aaaatcggca tcaataccgt
      301 catacggaca ttaaaaagct cccgccccgg cggataacat ctgcgccagt cgctcacgct
      361 gatgttgccc ttatctgtga acttgatggg cagtggagct tcgttggcag caaggcgcaa
      421 caacactgtc acgaacggtg caatagtgat ccacacccaa cgcctgaaat cagatccagg
      481 gggtaatctg ctctcctgat tcaggagagc ttatggtcac ttttgagaca gttatggaaa
      541 ttaaaatcct gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggctct
      601 cccgcaatac cgttaaacgt tatttgcagg caaaatctga gccgccaaaa tatacgccgc
      661 gacctgctgt tgcttcactc ctggatgaat accgggatta tattcgtcaa cgcatcgccg
      721 atgctcatcc ttacaaaatc ccggcaacgg taatcgctcg
//
LOCUS       DAZPFP010000461          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPFP010000461 DAZPFP010000000
VERSION     DAZPFP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.469"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHUR4_004378"
     CDS             <1..>759
                     /locus_tag="ACHUR4_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1457646.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZPFP010000462          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPFP010000462 DAZPFP010000000
VERSION     DAZPFP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.470"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            4..287
                     /locus_tag="ACHUR4_004379"
                     /pseudo
     CDS             4..287
                     /locus_tag="ACHUR4_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            369..506
                     /locus_tag="ACHUR4_004380"
                     /pseudo
     CDS             369..506
                     /locus_tag="ACHUR4_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          220 a          152 c          177 g          196 t
ORIGIN      
        1 cagttgacat ctaataagtt gcgggcttta gctaatgaac tagccaaaag tctcaaaaat
       61 cctgaagatc tcagtcagtt tgattggatg ctgaaaatga aaccgtactc aatgctaata
      121 tgactcatca tcttgggtgc gagaaaaatc agcttagatc tggcagcaat tcccgcaatg
      181 gttgcttaac gaagattatc actactggag atgaacctct ggaaatccgt actctgcgtg
      241 atcgtaatgg cactttcgaa ccgcaacagc tgaagaaaaa ccagccctag attactaaat
      301 ttgtcgggcg atggattaac tggttcaggc tgtctggtgc tgaaggcctg aagtcactgc
      361 tgtcagtgat gtggccggct cttcatgaga caataacccg taatcatcag tgccgttcaa
      421 tatggccact gttgaaaaaa gttcgtcact ttatggagac ggttagccca cttcccggag
      481 aaaaacatag tctggacaaa gtgtagcggt attaggctca catatttaga tgtagataag
      541 ccggagctgg cagttaaatc taggcagcat cacagaaatg gacaacgccc cttaaggact
      601 ggcgtatggc aatgagctgc tttattatca agtttggtga tcgccttgac ggtcacttct
      661 gagtgaaggc gcttgcacag aatggtaaac ggactcgatt attgaatctt agggataaga
      721 tatatctacg atatgtcaat atacg
//
LOCUS       DAZPFP010000463          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPFP010000463 DAZPFP010000000
VERSION     DAZPFP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.471"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHUR4_004381"
     CDS             complement(<1..158)
                     /locus_tag="ACHUR4_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1457647.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHUR4_004382"
     CDS             complement(308..469)
                     /locus_tag="ACHUR4_004382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457648.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHUR4_004383"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHUR4_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          196 a          216 c          144 g          187 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cct
//
LOCUS       DAZPFP010000464          742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPFP010000464 DAZPFP010000000
VERSION     DAZPFP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.472"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..65
                     /locus_tag="ACHUR4_004384"
                     /pseudo
     CDS             1..65
                     /locus_tag="ACHUR4_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(86..307)
                     /locus_tag="ACHUR4_004385"
                     /pseudo
     CDS             complement(86..307)
                     /locus_tag="ACHUR4_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(450..643)
                     /locus_tag="ACHUR4_004386"
                     /pseudo
     CDS             complement(450..643)
                     /locus_tag="ACHUR4_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
BASE COUNT          160 a          185 c          188 g          209 t
ORIGIN      
        1 acttccctta tgagttcaaa atcgccttaa tggagcagtc actgcaaccc agagcctgtg
       61 tggcgtagat cgcccgtgaa aatggaatct gccgttcggt ttttgcgcga agtttttctg
      121 agcttttacc gaactgcata cgctgcagtt tcgcaaccag cgccttcagt cggttgattt
      181 cggaagcata agccgccatc cgctgtgaga gcaggcggtt gtattcagcc atctggtgga
      241 tagtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc gttctcatga gcaagtgagg
      301 tgtccatttc ctcactttac aacgggttat atgcggattc cagcacgttc cgttcgtttt
      361 ggatgtttgt cgggtaagga cagttcattg ctggactgcc ctcccctaag aacggtgcat
      421 accggtttcc cggtacaccg ctcaagcctt tataagccct ttttgagaga gccggcttca
      481 caactgtgta actgtcgatg gcaatttgga tgtaacaaga caaggttaga taattcttca
      541 ccgcccccat aactttcttc accttatggt gtacgttcca gccagtttca tgggtaatgc
      601 gctgccggca tatcggacac aaagagcctt gtgactcgag taggcagcct gacggctgcg
      661 gtttgtcatg gtctgcaatt accgttataa aaacaggcaa tgtcattgtc tttcaggtgg
      721 ttatatggcc cgttcagcta aa
//
LOCUS       DAZPFP010000465          739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPFP010000465 DAZPFP010000000
VERSION     DAZPFP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.473"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>739
                     /locus_tag="ACHUR4_004387"
     CDS             <1..>739
                     /locus_tag="ACHUR4_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457649.1"
                     /translation="SPKGVIQFQYRYRWHGKPNRLGLGRYPSLSLKDARQITADLRKL
                     YFSGTDPRTYFEEKVENSMTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFP
                     NRPASSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPR
                     DFGSRAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTE
                     FDLLDNVWTVPKEHSKMGNVIRRPIFEQ"
BASE COUNT          196 a          182 c          190 g          171 t
ORIGIN      
        1 tcaccgaagg gggtcatcca gttccagtac cgctatcgct ggcatggcaa gcctaatcga
       61 cttgggcttg gtcgataccc atccctgtct ttgaaggatg ccagacagat cactgctgac
      121 ttgcgaaagc tctatttctc aggaacggat ccacgcacct attttgaaga gaaggtggag
      181 aactccatga cggtcgccca gtgtctcgac tactggttcg acaactacgt ctctacaact
      241 ctcagagaaa agacccaggc actttatcga tcaacggtta tgaagcgcat gcatgacgcc
      301 ttccctaatc gtccggcatc ttctatcacg gttaagcaat gggttgacct gcttaccgaa
      361 gaagaaaaag ataatccacg ccgagcaagg caggtgctaa gtcaactaag atcagcaatt
      421 agttggtgca tgcggcgtca gttgatagat agttgcgcaa ttatgagcat ccagccaagg
      481 gacttcggct cccgcgctga ggtaggggat cgggtactgt cgtatcacga actggctaag
      541 atttggcttg ctattgaaag aagccgtgcg tctacgtcaa ataagctcct tcatcagatg
      601 cttatgctgt ggggggcgag gctctcagag cttaggctgg caacaaagac agaatttgac
      661 ctgctggaca acgtatggac cgtaccgaaa gagcatagca agatgggtaa tgttatccgc
      721 cgtccaatct tcgaacaaa
//
LOCUS       DAZPFP010000466          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPFP010000466 DAZPFP010000000
VERSION     DAZPFP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.474"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            102..698
                     /gene="fimE"
                     /locus_tag="ACHUR4_004388"
     CDS             102..698
                     /gene="fimE"
                     /locus_tag="ACHUR4_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR1457650.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          197 a          142 c          180 g          217 t
ORIGIN      
        1 ttcgtgtgtg gttatctttt tatctattgg gctaattttg accgattgag gtttcctata
       61 ggtattcatt caaatatatc tcagttagga gtactactat tgtgagtaaa cgtcgttatc
      121 ttaccggtaa agaagttcag gccatgatgc aggcggtttg ttacggggca acgggagcca
      181 gagattattg tcttattctg ttggcatatc ggcatgggat gcgtattagt gaactgcttg
      241 atctgcatta tcaggacctt gaccttaatg aaggtcgaat aaatattcgc cgactgaaga
      301 acggattttc taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg gaacgctgga
      361 cccaggaacg tgctaactgg aaaggcgctg accggaccga cgctatattt atttctcgcc
      421 gcgggagtcg gctttctcgc cagcaggcct atcgcattat tcgcgatgcc ggtattgaag
      481 ctggaaccgt aacgcagact catcctcata tgttaaggca tgcttgcggt tatgaactgg
      541 cggagcgtgg tgcagatacc cgtttaattc aggattatct cgggcatcga aatattcgcc
      601 atactgtgcg ttataccgcc agtaatgctg ctcgttttgc cggattatgg gaaagaaata
      661 atctcataaa cgaaaaatta aaaagagaag aggtttgatt taacttattg ataataaagt
      721 taaaaaacaa ataaac
//
LOCUS       DAZPFP010000467          734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPFP010000467 DAZPFP010000000
VERSION     DAZPFP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.475"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(170..712)
                     /gene="fimA"
                     /locus_tag="ACHUR4_004389"
     CDS             complement(170..712)
                     /gene="fimA"
                     /locus_tag="ACHUR4_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1457651.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          216 a          156 c          151 g          211 t
ORIGIN      
        1 aatgattgct ttggcttctt acaacacatc attccctttt catttaataa agcggcatga
       61 tacaaagtga acgtggtttc gatttacatt gccaccatac cgcttcagac aaccggtaca
      121 ttactttgtt ataagacgtg atatataatt caaaacaacg tggttttgat tattcgtatc
      181 tcataataaa agtcgcgtca gcatttgctt gccctgctga tgcacttgta ccggtgcctt
      241 tataacgtgc agaaaaatga agaacgttgg tcccggggat caagttctgg ttggttgaga
      301 agctattccc atcaggtttg agaattgcgg atgtattatc aagtatttca ataccgacac
      361 ccgttgctgt tgttgtattt gtactggata caatggccaa taaattatca ttgattgcat
      421 cagcctgacc agaaaatgca acggcagcat tagcggcaac aacaggatcg cagtcgttca
      481 actgaatggt gaatggaatt aatgcagagg tattaccaac agcattgaaa atatcggtac
      541 gatattgacc gagtgtgaca acctggtctg ccgagtcagt attcactgaa caggcagcat
      601 tcaccagttc gccttcaaag tgaattgtac cgccatctac aacggcagca taactacccg
      661 tagcggcgaa tagtgcggca gccagcgcag acgaaataaa tcttaatttc atatatattc
      721 cttcaatctc attt
//
LOCUS       DAZPFP010000468          725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPFP010000468 DAZPFP010000000
VERSION     DAZPFP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.476"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            60..>725
                     /locus_tag="ACHUR4_004390"
     CDS             60..>725
                     /locus_tag="ACHUR4_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457652.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQD"
BASE COUNT          280 a          142 c          117 g          186 t
ORIGIN      
        1 tgttctaatt atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta
       61 tgtcaattga ctttacccca ggtataataa atacatatca cggcgatatt tataactgca
      121 caacaaatac cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg
      181 aagaacaaca acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg
      241 atttagcgca acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa
      301 atgcagacta ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga
      361 taagttcgac accacttacg caacagacac aatcaatact taatcagatt cataacatta
      421 gatacgaaaa ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca
      481 acaatccaat cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc
      541 aaaccgtttc ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata
      601 taagagacat tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa
      661 atgaaaatag gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc
      721 aagat
//
LOCUS       DAZPFP010000469          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPFP010000469 DAZPFP010000000
VERSION     DAZPFP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.477"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            44..>714
                     /locus_tag="ACHUR4_004391"
     CDS             44..>714
                     /locus_tag="ACHUR4_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1457653.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          235 a          121 c          160 g          198 t
ORIGIN      
        1 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
       61 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      121 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      181 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      241 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      301 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      361 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      421 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      481 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
      541 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
      601 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
      661 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aaga
//
LOCUS       DAZPFP010000470          707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPFP010000470 DAZPFP010000000
VERSION     DAZPFP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.478"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHUR4_004392"
     CDS             <1..243
                     /locus_tag="ACHUR4_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457654.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>707
                     /locus_tag="ACHUR4_004393"
     CDS             358..>707
                     /locus_tag="ACHUR4_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457655.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          189 a          181 c          184 g          153 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaat
//
LOCUS       DAZPFP010000471          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPFP010000471 DAZPFP010000000
VERSION     DAZPFP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.479"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHUR4_004394"
     CDS             complement(<1..443)
                     /locus_tag="ACHUR4_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1457656.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZPFP010000472          700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPFP010000472 DAZPFP010000000
VERSION     DAZPFP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.480"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..617)
                     /locus_tag="ACHUR4_004395"
     CDS             complement(<1..617)
                     /locus_tag="ACHUR4_004395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1457657.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGY"
BASE COUNT          212 a          158 c          126 g          204 t
ORIGIN      
        1 taataacctt ccggccaaca caaatgttta ctgcattttc ctgtcatttc tttcaaatat
       61 tccctcccgc tcaacaagtc ctgccgaaga tgtttacact gttcttctcg tgaactgaac
      121 tttttatccc agcgtgtatg agtatgcgta tgaacatgaa attcgaccag accagattgc
      181 aacatttcgt ttacttcaga ccagcgaagc ataacattat cagcattccc tgtcgctatt
      241 tgatgttcgc aatcgcgatg agaatattcc tttccaggag agtgacgaac cggaccattc
      301 ccaataaaac cggtaattaa aaaaatatgt gctttgagat taaactcttt taataaagga
      361 tatacctgaa accagttgtc aaggtatcca tcatcaaatg tcaacataac gcttttccgg
      421 gggagttttc cgccccgata aaaaaactcc agttcatctg atgacagtgt tttccagttg
      481 ttttcagcca accattttat ttgcttccgg aatgtcacgg gagaaagtgt aaccagtccg
      541 gggcaatgac tgacatgatg atacattaat accggaacat gattggcttt gaacaaaatg
      601 cccccttcgt ttaacattta aagtttctgg cacctagcaa gcagagacaa aaggaaaacc
      661 caataaagct ggtgcatttt atttccagat actggatgaa
//
LOCUS       DAZPFP010000473          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPFP010000473 DAZPFP010000000
VERSION     DAZPFP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.481"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHUR4_004396"
     rRNA            <1..673
                     /locus_tag="ACHUR4_004396"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZPFP010000474          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPFP010000474 DAZPFP010000000
VERSION     DAZPFP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.482"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHUR4_004397"
     CDS             32..493
                     /locus_tag="ACHUR4_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1457658.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>695)
                     /locus_tag="ACHUR4_004398"
     CDS             complement(534..>695)
                     /locus_tag="ACHUR4_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1457659.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          159 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaat
//
LOCUS       DAZPFP010000475          694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPFP010000475 DAZPFP010000000
VERSION     DAZPFP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.483"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            95..550
                     /gene="csgB"
                     /locus_tag="ACHUR4_004399"
     CDS             95..550
                     /gene="csgB"
                     /locus_tag="ACHUR4_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1457660.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            591..>694
                     /locus_tag="ACHUR4_004400"
     CDS             591..>694
                     /locus_tag="ACHUR4_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR1457661.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT          211 a          126 c          173 g          184 t
ORIGIN      
        1 tcgtaacgca gcgttaacaa aatacaggtt gcgttaacaa ccaagttgaa atgatttaat
       61 ttcttaaatg tacgaccagg tccagggtga caacatgaaa aacaaattgt tatttatgat
      121 gttaacaata ctgggtgcgc ctgggattgc agccgcagca ggttatgatt tagctaattc
      181 agaatataac ttcgcggtaa atgaattgag taagtcttca tttaatcagg cagccataat
      241 tggtcaggct gggactaata atagtgctca gttacggcag ggaggctcaa aacttttggc
      301 ggttgttgcg caagaaggta gtagcaatcg ggcaaagatt gaccagacag gagattataa
      361 ccttgcatat attgatcagg cgggcagtgc caacgatgcc agtatttcgc aaggtgctta
      421 tggtaatact gcgatgatta tccagaaagg ttctggtaat aaagcaaata ttacacagta
      481 tggtactcaa aaaacggcag ttgtagtgca gagacagtcg caaatggcaa ttcgcgtgac
      541 acaacgttaa ttaccattcg acttttaaat caatccgatg ggggttttac atgaaacttt
      601 taaaagtagc agcaattgca gcaatcgtat tctccggtag cgctctggca ggtgttgttc
      661 ctcagtacgg cggcggcggt aaccacggtg gtgg
//
LOCUS       DAZPFP010000476          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPFP010000476 DAZPFP010000000
VERSION     DAZPFP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.484"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHUR4_004401"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHUR4_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZPFP010000477          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPFP010000477 DAZPFP010000000
VERSION     DAZPFP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.485"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUR4_004402"
     CDS             complement(<1..251)
                     /locus_tag="ACHUR4_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR1457662.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHUR4_004403"
     CDS             complement(272..>679)
                     /locus_tag="ACHUR4_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457663.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZPFP010000478          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPFP010000478 DAZPFP010000000
VERSION     DAZPFP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.486"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            205..>677
                     /locus_tag="ACHUR4_004404"
     CDS             205..>677
                     /locus_tag="ACHUR4_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR1457664.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT          187 a          138 c          161 g          191 t
ORIGIN      
        1 gccaacaatc ttgcaaaaaa aatgcgattg ctaaaacaaa agtgcaattt gcggcaatat
       61 agttattcca tgaccgtaca atcttgcatt cagtgtaaat aatcttgttt agctctcgct
      121 aaacgcggtt gtataaccgc aatacacgcc gtattttatc cggcatatat tgtttttgct
      181 tgctcatgga atagggaggt tgtcatgtat caacgctgtt ttgataatgc cagtgaaacg
      241 ctgtttgtcg ccggtaaaac gccacggctt tcacgttttg catttagcga tgatccaaaa
      301 tgggagtctg gacatcacgt tcatgacaat gaaaccgagc tgatttacgt caagaaaggg
      361 gttgcaaggt ttaccatcga ttcttcgtta tatgtcgcac atgcggatga cattgtggtg
      421 atagaacgcg gcaggctgca tgcggtggcc tctgacgtta acgatccggc aacgacgtgt
      481 acctgtgcgc tgtacggctt tcagtttcag ggggctgagg aaaatcagtt actgcaaccg
      541 cattcttgtc cggtaattgc cgcggggcag ggaaaagaag tcataaaaac cttatttaat
      601 gagctaagtg tgattttgcc gcaaagtaaa aatagccaaa catcttcgtt atgggatgca
      661 ttagcctata cattagc
//
LOCUS       DAZPFP010000479          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPFP010000479 DAZPFP010000000
VERSION     DAZPFP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.487"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHUR4_004405"
     CDS             complement(<1..417)
                     /locus_tag="ACHUR4_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1457665.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQ"
     gene            complement(427..>669)
                     /locus_tag="ACHUR4_004406"
     CDS             complement(427..>669)
                     /locus_tag="ACHUR4_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1457666.1"
                     /translation="GALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISG
                     GTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          161 a          194 c          157 g          157 t
ORIGIN      
        1 ttgcattgct tctggagacg atgacgcaag gcgtgcgcca gccgctaagg tgccgataga
       61 attacgctgc tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
      121 atattgagta gccaactgac gcaaagcacc gcgatcacca gacgcataag cattagcgta
      181 cgcctgctga aattctttct gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat
      241 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcgcctgaac gctcaatatc
      301 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
      361 gttgacgccg ccgataccag ccaacaatcc gccgtttgtt ccttgccaag tagccatgat
      421 tcccccttaa aacaacgagc caagcaatcc gataccagca ccaataccag cgccccaagg
      481 cgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgtgccacc
      541 gctaattgca gtctgaagac ttgatggttt attggcatta gcagcggcaa gagctgcgct
      601 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
      661 cagcgcacc
//
LOCUS       DAZPFP010000480          666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPFP010000480 DAZPFP010000000
VERSION     DAZPFP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.488"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>666
                     /locus_tag="ACHUR4_004407"
     CDS             <1..>666
                     /locus_tag="ACHUR4_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1457667.1"
                     /translation="GGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDISNV
                     WARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIGGSY
                     SVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAETNPT
                     TYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASLFEVT
                     QWD"
BASE COUNT          210 a          133 c          139 g          184 t
ORIGIN      
        1 ctggcggact cattgctgtt tctgaattag cgaaaagagt ttctggtaaa acaaaccgaa
       61 aacttgtagc aacaatgttg tctctggctg ttgcaggtac agtaaatgca gcaaatattg
      121 atatatcaaa tgtatgggcg agagactatc ttgatcttgc acaaaataaa ggtattttcc
      181 agcccggagc aacagacgta acaatcactt taaaaaacgg agataaattc tctttccata
      241 atctctcaat tccggatttt tctggtgcag cagcgagtgg cgcagctacc gcaataggag
      301 gttcttatag tgttactgtt gcacataaca aaaagaaccc tcaggccgca gaaactcagg
      361 tttacgctca gtcttcttac aaggttgttg acagaagaaa ttccaatgat tttgagattc
      421 agaggttaaa taaatttgtt gtggaaacag taggtgccac cccggcagag accaacccta
      481 caacatattc tgatgcatta gaacgctacg gtatagtcac ttctgacggt tcaaaaaaaa
      541 tcataggttt tcgtgctggc tctggaggaa catcatttat taatggtgaa tccaaaatct
      601 caacaaattc agcatatagc catgacctgt taagtgctag tctatttgag gtcacccaat
      661 gggact
//
LOCUS       DAZPFP010000481          663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPFP010000481 DAZPFP010000000
VERSION     DAZPFP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.489"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            307..382
                     /locus_tag="ACHUR4_004408"
     tRNA            307..382
                     /locus_tag="ACHUR4_004408"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Ile,seq:cat)
BASE COUNT          196 a          116 c          156 g          195 t
ORIGIN      
        1 atggagtgaa aagtaacaga tatcataatg atgcgaaaac tcttcagcta atgtaataaa
       61 gttatattat tagatacaaa atcattacaa agtatcattg aatcgatgat aactatagat
      121 agtgctaatt attaacattt gcttgttgac tgcgagtatc gactttgcga atggaggcgg
      181 tgtcgggttt tatgtcgcgt gtaatcacga cccaaggtga atgggaacgc ataaaaatct
      241 gttatttaca tcataagatc atcatgttta ttgcgtggtt tataagtatt gttattgcat
      301 acttaaggcc ctttagctca gtggttagag caggcgactc ataatcgctt ggtcgctggt
      361 tcaagtccag caagggccac caaatcatca ttcatgtatc aggaagagtt aagggtacaa
      421 ggggtactcg atggcggtct atatttcatg actaacgcca tttcaaggaa gaagtgcggt
      481 gggtggtttc gaatgagggc tggtgttttg gtacgtggca cgccactgca atgtatttat
      541 tcatcagtac tcattcaaaa gtacctggaa aaaatgcccg ctatgccaag cgggcaaatt
      601 gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc aaattgttcc
      661 gga
//
LOCUS       DAZPFP010000482          658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPFP010000482 DAZPFP010000000
VERSION     DAZPFP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.490"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /gene="virK"
                     /locus_tag="ACHUR4_004409"
     CDS             complement(<1..262)
                     /gene="virK"
                     /locus_tag="ACHUR4_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1457668.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWT"
     gene            complement(267..>658)
                     /gene="capU"
                     /locus_tag="ACHUR4_004410"
     CDS             complement(267..>658)
                     /gene="capU"
                     /locus_tag="ACHUR4_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1457669.1"
                     /translation="QYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMV
                     LAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMA
                     RLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          202 a          150 c          133 g          173 t
ORIGIN      
        1 gggtccaggc gcgcccccct ttatgcaaat aagggcgatg aattcttgct ggcaacgtgg
       61 ggtgtattgc cagaatatcc tcaatgcacg gcaaggaacg cagttcataa taatatcgaa
      121 tactgacaac cggatttata gaacatcgca aaatgtacat gaaccgaaat tttctgtgtt
      181 cccacttccc cggtagtgaa tctgaggaaa aaataatcct tttaaggaaa ccgataaatg
      241 ataagttact tatagaaaac atattgttac ttttgcttcg ctaatgtgag tatttttaac
      301 gcagttttat taatatcaaa ccgctcttct atatcctgtt ttgccaggcg agccatctga
      361 taaaaacgcc cagggtcatt aaaaaaatca ttcagggcgc acatccatgc gtgcttatta
      421 cttgctggta acaatgtccc ggtctggttg ttctgaataa cctcagggat tccaccaatc
      481 tgagtggcca ctacaggcac agaaaatgcc gatgcttctg ccagcaccat accaaaagat
      541 tcgttttctg aaggcagaac caccagactg gcaacccgat atacgggggc ggcaggaaaa
      601 acattgtcag caataaaaac atcatcatgc atgcccatac tgtcaatctg atactgta
//
LOCUS       DAZPFP010000483          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPFP010000483 DAZPFP010000000
VERSION     DAZPFP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.491"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHUR4_004411"
     CDS             <1..>656
                     /locus_tag="ACHUR4_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457670.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZPFP010000484          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPFP010000484 DAZPFP010000000
VERSION     DAZPFP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.492"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHUR4_004412"
     CDS             complement(151..>656)
                     /locus_tag="ACHUR4_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1457671.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZPFP010000485          654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPFP010000485 DAZPFP010000000
VERSION     DAZPFP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.493"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..134
                     /locus_tag="ACHUR4_004413"
     CDS             <1..134
                     /locus_tag="ACHUR4_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1457672.1"
                     /translation="MNTGVPGNKVMKKLPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            144..506
                     /gene="ipgE"
                     /locus_tag="ACHUR4_004414"
     CDS             144..506
                     /gene="ipgE"
                     /locus_tag="ACHUR4_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1457673.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            506..>654
                     /gene="ipgF"
                     /locus_tag="ACHUR4_004415"
     CDS             506..>654
                     /gene="ipgF"
                     /locus_tag="ACHUR4_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1457674.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAV"
BASE COUNT          210 a           83 c          151 g          210 t
ORIGIN      
        1 agatgaatac tggtgtgccc ggaaataaag tcatgaaaaa attgccgcta tcttcgttag
       61 agctatctta ttctgaaaga ataggggacc caaaaatatg gaatatggtg aaagggtatt
      121 cgtcatttgt ataagaggaa tatatggaag atttagcaga tgttatttgc cgagccttgg
      181 gtatcccttt aattgatatc gatgatcaag caatcatgct tgatgatgat gtgctaatat
      241 atattgaaaa agaaggagat tcaataaatt tattatgccc tttttgtgca ttgcctgaaa
      301 atataaatga tcttatatat gcgttgagcc taaattactc agaaaagata tgcttagcta
      361 ctgatgatga aggcggaaat ctaattgcgc gattagatct taccgggata aatgaattcg
      421 aggatgtata tgttaataca gaatattata tttcgcgtgt gcgttggctg aaggatgaat
      481 ttgcgcgaag aatgaagggg tattaatgtc ccgtttcgta tttattttgc tgtgttttat
      541 cccatattta gggagagctg attgttggga taaggctggt gaaaggtaca atattccatc
      601 aagtttgtta aaagcgattg cggaaaaaga atccggattt aataaatctg cagt
//
LOCUS       DAZPFP010000486          653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPFP010000486 DAZPFP010000000
VERSION     DAZPFP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.494"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHUR4_004416"
     CDS             <1..357
                     /locus_tag="ACHUR4_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1457675.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT          201 a          146 c          150 g          156 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat agg
//
LOCUS       DAZPFP010000487          652 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPFP010000487 DAZPFP010000000
VERSION     DAZPFP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.495"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            316..>652
                     /gene="virA"
                     /locus_tag="ACHUR4_004417"
     CDS             316..>652
                     /gene="virA"
                     /locus_tag="ACHUR4_004417"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1457676.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQL"
BASE COUNT          229 a          128 c           92 g          203 t
ORIGIN      
        1 tactatgata gtctgcttca gaccgcaact tttcagttct ttctgtacgc ttgcccaaag
       61 tttaaccact ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc
      121 caaagccaat aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa
      181 taactgcata attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc
      241 cattttatta tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac
      301 atcaggagaa atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca
      361 tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac
      421 attttactga aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa
      481 catagcgaaa aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac
      541 ggagagtttg cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc
      601 atatgttcgg ttttttatct tgatggagat aaaatacaat ccactcaact tt
//
LOCUS       DAZPFP010000488          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPFP010000488 DAZPFP010000000
VERSION     DAZPFP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.496"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUR4_004418"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUR4_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR1457677.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUR4_004419"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUR4_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1457678.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZPFP010000489          638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPFP010000489 DAZPFP010000000
VERSION     DAZPFP010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.497"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..470
                     /locus_tag="ACHUR4_004420"
     CDS             <1..470
                     /locus_tag="ACHUR4_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR1457679.1"
                     /translation="VTEGQTPFKSSVTISSGTMVSKEVFEIVGMMDEELFIDYVDTEW
                     CLRCLNYGILVHIIPDIEMVHAIGDKSVKICGINIPIHSPVRRYYRVRNAFLLLRKNH
                     VPLLLSIREVVFSLIHTTLIIATQKNKIEYMKKHILATLDGIRGITGGGRYNA"
     gene            463..>638
                     /locus_tag="ACHUR4_004421"
     CDS             463..>638
                     /locus_tag="ACHUR4_004421"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1457680.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQ"
BASE COUNT          219 a           85 c          121 g          213 t
ORIGIN      
        1 atgtcactga aggacagaca ccgtttaaaa gttcagtaac aatctcatcc ggaaccatgg
       61 tttcaaaaga agtttttgag attgttggaa tgatggatga ggaacttttt attgattatg
      121 tcgatacaga atggtgtctt agatgcttaa actatggcat attagttcat atcattcctg
      181 atatagaaat ggttcatgct attggggata agtcagtaaa aatctgtggg attaacatac
      241 caattcactc gccagtacgt cgttattatc gagtaagaaa tgcatttctt ttgcttagaa
      301 aaaatcatgt gcctctttta ctctctatta gggaagttgt tttttcttta attcatacga
      361 ctttaattat cgcaactcaa aaaaataaaa ttgaatatat gaaaaaacat attttggcta
      421 cgctggatgg aataagagga attactgggg gcggtcgtta taatgcatag ttctgatcaa
      481 aaaagagtag ctgtacttat ggctacgtat aatggcgaat gctggattga ggaacaatta
      541 aaaagtatta ttgaacaaaa agatgtagac atctcaattt ttatatcaga tgatttatca
      601 actgataata cattaaatat ttgtgaagaa tttcaatt
//
LOCUS       DAZPFP010000490          631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPFP010000490 DAZPFP010000000
VERSION     DAZPFP010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.498"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>631
                     /locus_tag="ACHUR4_004422"
     CDS             <1..>631
                     /locus_tag="ACHUR4_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457681.1"
                     /translation="YLVTCQLNEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLL
                     TLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFS
                     RPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWS
                     AFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQP"
BASE COUNT          199 a          125 c          128 g          179 t
ORIGIN      
        1 atatctggta acttgtcagt tgaatgaaga acaaatgtca tcgctgcttt atgaaagaga
       61 tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagcct tttcattcgg
      121 gggagatctc ctaacccttg catcaaaacc gggttccaca atctcaagca tcccatcaga
      181 aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc ttttcaatga
      241 ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg ccgctctccc
      301 gttcagcagg cctttagttt actgggatgg ccaggtatta aaatcaacga ctgaaattaa
      361 aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg cttaataaag gttgctctac
      421 ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag gaatatggag
      481 cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg gggattctgt
      541 gaacattaat cactctcctc atgatggact agttataata aataaaggaa acgaagaagt
      601 tgaaggtacc tggcctaaca aattgcaacc t
//
LOCUS       DAZPFP010000491          629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPFP010000491 DAZPFP010000000
VERSION     DAZPFP010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.499"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..165
                     /locus_tag="ACHUR4_004423"
                     /pseudo
     CDS             1..165
                     /locus_tag="ACHUR4_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(182..629)
                     /locus_tag="ACHUR4_004424"
                     /pseudo
     CDS             complement(182..629)
                     /locus_tag="ACHUR4_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          152 a          149 c          172 g          156 t
ORIGIN      
        1 gcagaaaaac ggcttgcggt caggcaggag aaatgtgtgc cgcgaatgaa gtccttgtat
       61 gactggataa agctccagag gaaaactttg tcaaaccatg ctgaaatggt aaaggcgttc
      121 aactatatcc tgaatcactg gaatgcactg aatgagtttt gccgggtagt acttccaatc
      181 attagaatat gtgcttttca atgaaggaac cgatgacttt ttcgtggatt tccacggaac
      241 gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggggcggagg gtcaggttgt
      301 tacgttcata cgttgcgtga agattttgcc ggtcaggtgt ttctcctccg gcacttctct
      361 ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag tcaacagggc
      421 taacagttcc cgacaggtct catcattccg tggcccgcaa gtgtaagcca acacaccgcc
      481 agttttagtg ttgtacgcat accagggcca gtgttgttgc gccttgctgc caacgaagcg
      541 ccactgctca tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt
      601 tatccgccgg ggcgagagct ttttaatgt
//
LOCUS       DAZPFP010000492          628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPFP010000492 DAZPFP010000000
VERSION     DAZPFP010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.500"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(104..628)
                     /locus_tag="ACHUR4_004425"
                     /pseudo
     CDS             complement(104..628)
                     /locus_tag="ACHUR4_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          172 a          151 c          126 g          179 t
ORIGIN      
        1 cttcagtttt aaactcttcg ggataacgct taccgctcat gggcacctct ctttaagcca
       61 tcttaaatga ctctgaggtg tctgttaaac ccgtggcgat tcaggtctgc atagcattat
      121 caacattcgg atgtgcaact acagctaatg caacatcatc ccagcttgca atagtgctat
      181 cagcaacacc atctccgtca gtatctacgg tgacaaagtt agatttacca ctataatcgc
      241 tggcattacc tgtattaccg aaccaaccag tagtaccttc atcagtccat gaaccagagg
      301 ttacggtaga atctttaaca gtgatggtgt tattaaacac ttcgctgctg tttacgccag
      361 tgctgacagt gttgtcgtca atatcttccc actcataacc ctgagtcaac gtaatacccg
      421 caacgtgaga gttattagtg atggacagat caacttcctg atccagagta atagccgtac
      481 caatgttgta aacgtctaca acatgagtat cagcggcatt attgtaagtg ccgttatatg
      541 tgtagtgttc gtagttgtcg tcgatcgttg aattatcaac agtcagcgcc agacggtcat
      601 ggtagtaatc atccgcacga tcagcaca
//
LOCUS       DAZPFP010000493          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPFP010000493 DAZPFP010000000
VERSION     DAZPFP010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.501"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..534
                     /locus_tag="ACHUR4_004426"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACHUR4_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          150 a          169 c          153 g          147 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcat
//
LOCUS       DAZPFP010000494          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPFP010000494 DAZPFP010000000
VERSION     DAZPFP010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.502"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            20..619
                     /locus_tag="ACHUR4_004427"
                     /pseudo
     CDS             20..619
                     /locus_tag="ACHUR4_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459355.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          179 a          108 c          136 g          196 t
ORIGIN      
        1 ctcacttgag ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc
       61 tcgaaatgta ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa
      121 cttagctact ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc
      181 tattgataaa aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg
      241 ctgtgtgcgt atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg
      301 ctcaaccatt ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg
      361 gtgtaaaaaa cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa
      421 tatgctggca taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg
      481 atcatcacct gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg
      541 gtgtcgttac gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta
      601 agataagtgt taaagttaa
//
LOCUS       DAZPFP010000495          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPFP010000495 DAZPFP010000000
VERSION     DAZPFP010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.503"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..237
                     /gene="ipgC"
                     /locus_tag="ACHUR4_004428"
     CDS             <1..237
                     /gene="ipgC"
                     /locus_tag="ACHUR4_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR1457682.1"
                     /translation="AIYQIKEQFQQAADLYAVAFALGKNDYTPVFHTGQCQLRLKAPL
                     KAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            243..>619
                     /locus_tag="ACHUR4_004429"
     CDS             243..>619
                     /locus_tag="ACHUR4_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HFR1457683.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTL"
BASE COUNT          234 a          134 c           86 g          165 t
ORIGIN      
        1 gctatttatc agataaaaga acagttccaa caagcagcag acctttatgc tgtcgctttt
       61 gcattaggaa aaaatgacta tacaccagta ttccatactg gacaatgcca gcttcggttg
      121 aaagccccct taaaagctaa agagtgcttc gaactcgtaa ttcaacacag caatgatgaa
      181 aaattaaaaa taaaagcaca atcatacttg gacgcaattc aggatatcaa ggagtaatta
      241 ttatgcataa tgtaagcacc acaaccactg gttttcctct tgccaaaata ttggcttcca
      301 ctgagcttgg agacaatact atccaagctg caaatgatgc agctaacaaa ttattttctc
      361 ttacaattgc tgatcttact gctaaccaaa atattaatac aactaatgca cactcaactt
      421 caaatatatt aatccctgaa cttaaagcac caaagtcatt aaatgcaagt tcccaactaa
      481 cgcttttaat tggaaacctt attcaaatac tcggtgaaaa atctttaact gcattaacaa
      541 ataaaattac tgcttggaag tcccagcaac aggcaagaca gcaaaaaaac ctagaattct
      601 ccgataaaat taacactct
//
LOCUS       DAZPFP010000496          616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPFP010000496 DAZPFP010000000
VERSION     DAZPFP010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.504"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            171..>616
                     /locus_tag="ACHUR4_004430"
     CDS             171..>616
                     /locus_tag="ACHUR4_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457684.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQG"
BASE COUNT          233 a          128 c           83 g          172 t
ORIGIN      
        1 tttaaaaaat cagcattcat tggttaatct ggaagcgcaa aataatagcc taaaaaaatg
       61 atatttctga tagttataaa ctgaaatttc ttaatcttga ttataacaag ctagcatcgc
      121 tggatgtctc ccggcaagaa tctctgattg agttaagtgc ccatcacaat atgatcactg
      181 accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac aaccatattg
      241 cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc aataacaatt
      301 tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg catgtattag
      361 ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca gtaagaaata
      421 taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta accactcaac
      481 caccagggct gcaaaacttc acctctatca atcttgataa taaccaactc acacattttg
      541 atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa
      601 taaattttcc tcaagg
//
LOCUS       DAZPFP010000497          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPFP010000497 DAZPFP010000000
VERSION     DAZPFP010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.505"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            343..534
                     /locus_tag="ACHUR4_004431"
     CDS             343..534
                     /locus_tag="ACHUR4_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457685.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          177 a          172 c          145 g          120 t
ORIGIN      
        1 aaatatgcac ctcccaccgc aagcggcggg cccccaaccg aagccggttt agttacaaca
       61 tgcaaacata aacccctaaa aaacccaagt ccaacgcaaa actgaaacca caacgccccc
      121 ctcataactg aaaagcgccg ccgcccccgc ccaaagggcg ggaacagagt cgcttttaat
      181 tatgaatgtt gtaactacat catcatcgct gtcagtcttc tggctggacg tatcgaatac
      241 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac ttcgggtaaa
      301 accctcgtcg ggaccactcc gaccgcgcac agaagctcta tcatggctga aaacgggtat
      361 ggcttggcag ggctggggat gggtaaggtg aaatctgtca atcagtaccg gctgacgccg
      421 ggcttcggcg gttttactcc ggtatcacat gtaaccacag cgtgccgcct tccatgccgc
      481 tggcgcggca tcagaataat acaagcagca tttaatgcat ttgcaaaagt gtgagtaata
      541 ttaagtggtc gtttcaggga tatcgttaag acttgagcga taggaattaa aaccccaaaa
      601 agattaaaaa aaca
//
LOCUS       DAZPFP010000498          607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPFP010000498 DAZPFP010000000
VERSION     DAZPFP010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.506"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..234
                     /locus_tag="ACHUR4_004432"
                     /pseudo
     CDS             1..234
                     /locus_tag="ACHUR4_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            348..606
                     /locus_tag="ACHUR4_004433"
                     /pseudo
     CDS             348..606
                     /locus_tag="ACHUR4_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          206 a          120 c          153 g          128 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggaaggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atataga
//
LOCUS       DAZPFP010000499          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPFP010000499 DAZPFP010000000
VERSION     DAZPFP010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.507"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>585)
                     /locus_tag="ACHUR4_004434"
     CDS             complement(<1..>585)
                     /locus_tag="ACHUR4_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1457686.1"
                     /translation="LIISLLSFMLSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRC
                     MFLELTRDRFFGKSKIGFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQA
                     INTIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFS
                     VTIPNPICAIIYTCICVYVSDKIGHWLSRTVGGPF"
BASE COUNT          206 a           97 c          102 g          180 t
ORIGIN      
        1 aaatggccca ccgactgtcc tcgatagcca atggccaatt ttatctgata catatacaca
       61 aatacaggta tatatgattg cgcagatagg atttggaata gtcacgctga agcctataat
      121 tgctaaagcc atagggataa ataatttggt gatatactcc attccacata tgttaagagt
      181 atttcttcca gctcttacaa taaagtcatt tccacaaatt atttttgcta tgaaaatatt
      241 aaagattatc aagcctaccg tggtgataat agtattaata gcttgaatat aactacttga
      301 tattatgtct gcattgaata ttgagataat cttaaaaagt aaaggtttct ggttaagtag
      361 ttgatacgca ctcattatag cggtaattga gaagacaatg aaaccaattt tagattttcc
      421 aaaaaacctg tcacgtgtta attctaaaaa catacatcta ccgagaggca ataaccacca
      481 gtatgccata gcggagtcta tgttccaaaa ccattgagga tctagtaatg ggttatgatt
      541 gagcactgtt tgtgacaaca tgaacgataa cagtgatatg attaa
//
LOCUS       DAZPFP010000500          582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPFP010000500 DAZPFP010000000
VERSION     DAZPFP010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.508"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..83)
                     /locus_tag="ACHUR4_004435"
     CDS             complement(<1..83)
                     /locus_tag="ACHUR4_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1457687.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQW"
     gene            complement(241..>582)
                     /locus_tag="ACHUR4_004436"
     CDS             complement(241..>582)
                     /locus_tag="ACHUR4_004436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457688.1"
                     /translation="MHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQCSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPSGEEDRRKKDPPAKTLGMNSFRY
                     SDCWRVKFLMN"
BASE COUNT          170 a          144 c          116 g          152 t
ORIGIN      
        1 aaccactgca aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt
       61 gatatttttt ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga
      121 aaaaaggcca tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa
      181 aacacgcacg actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc
      241 ttaattcatg aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa
      301 tgtttttgct ggcgggtcct tttttctacg gtcctcctcc ccggaggggc atttcattat
      361 ttttctaatc gttactggtg agaaggcgga agaaaaagcc agttcttcgc ccctaaataa
      421 cagcatgatt ccagtccatg caaaatctga tttccagctc acacataccg agcactgtac
      481 aaaaaaccgc cagccaaggc agttaagagt gtgttcccgg ggtttgctta gaatattttg
      541 taagttgtcc gaagagatat ttacaacacc agaatgatgc at
//
LOCUS       DAZPFP010000501          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPFP010000501 DAZPFP010000000
VERSION     DAZPFP010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.509"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(141..>581)
                     /locus_tag="ACHUR4_004437"
     CDS             complement(141..>581)
                     /locus_tag="ACHUR4_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1457689.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAZPFP010000502          580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPFP010000502 DAZPFP010000000
VERSION     DAZPFP010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.510"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUR4_004438"
     CDS             <1..318
                     /locus_tag="ACHUR4_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457690.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..>580
                     /locus_tag="ACHUR4_004439"
     CDS             321..>580
                     /locus_tag="ACHUR4_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1457691.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR"
BASE COUNT          178 a          122 c          149 g          131 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg
//
LOCUS       DAZPFP010000503          578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPFP010000503 DAZPFP010000000
VERSION     DAZPFP010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.511"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUR4_004440"
     CDS             complement(<1..188)
                     /locus_tag="ACHUR4_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457692.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKC"
     gene            complement(210..578)
                     /locus_tag="ACHUR4_004441"
                     /pseudo
     CDS             complement(210..578)
                     /locus_tag="ACHUR4_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           93 c          111 g          196 t
ORIGIN      
        1 atgcattttc cacttggttg taacgcgtta agtaaagtga ccatttgaat agaggtctgt
       61 tttagttttt ctatacctgg tgctagtgct tcgaggttat ttcctatggg gctttcagga
      121 aggtcattca tttttcgacg gactaaatca ctactaatat cattaccaat tgatgaaaca
      181 gggaacatta ttttatcctt tataaataat caataaaaat cattattaaa atgatcgggt
      241 gaaaaaaaga gaatttgcca acaatctgaa gtaatgcagg ttttattaaa gtgcctaggg
      301 ggtgttaaga tggcttcttt ttgggtgaac tttgcgcaat acttagaaat cgtctcctta
      361 atacattcca gctctttttt attgacggct gagcaagtac attgttcacc aaaaggtgca
      421 aaagtgccta catctaatgt ttgtatgata taatcatcat gttctcctgt agaaatatct
      481 atgatgaatg gactcatatt ttccggttgg attcggaatc atgacagaag gaacatcgcc
      541 ttgatagaaa ttcaatgcag taattgcaga taatactc
//
LOCUS       DAZPFP010000504          573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPFP010000504 DAZPFP010000000
VERSION     DAZPFP010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.512"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            177..545
                     /locus_tag="ACHUR4_004442"
                     /pseudo
     CDS             177..545
                     /locus_tag="ACHUR4_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          173 a          131 c          106 g          163 t
ORIGIN      
        1 aatttgatgc gtgtttttct ccataaatta tatatgtcca cattcggact taggggaaag
       61 aataattgaa ccattcgtct gtaacgcagc ataatcgtta gcgcgaaaca taatatgttt
      121 tctatgcatt gataattgat ggatcaactt attacgtccc tgaggaggga tgacaaatgc
      181 actcctggaa aaagaaactt gtagtatcac aattagcatt ggcttgcact ctggctatca
      241 cctctcaggc taatgcagcg accaacgata tttctggtca aacttacaat actttccatc
      301 actacaacga cgccacctat gctgatgacg tttactatga tggttatgta ggctggaaca
      361 actatgccgc tgatagctat tacaacggcg atatctaccc ggtcattaat aacgctaccg
      421 ttaacggcgt gatttctacc tactatctgg acgacggtat ttctaccaat accaacgcca
      481 atagtctgac aatcaaaaac agcactattc acggtatgat ttattccgag tgcatgacaa
      541 ctgattagac tggccccctg aatctccaga caa
//
LOCUS       DAZPFP010000505          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPFP010000505 DAZPFP010000000
VERSION     DAZPFP010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.513"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHUR4_004443"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHUR4_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1457693.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>568)
                     /locus_tag="ACHUR4_004444"
     CDS             complement(405..>568)
                     /locus_tag="ACHUR4_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1457694.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          105 g          145 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaatg
//
LOCUS       DAZPFP010000506          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPFP010000506 DAZPFP010000000
VERSION     DAZPFP010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.514"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..568)
                     /locus_tag="ACHUR4_004445"
                     /pseudo
     CDS             complement(1..568)
                     /locus_tag="ACHUR4_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          125 a          157 c          143 g          143 t
ORIGIN      
        1 tcccagtcgt gagcctctgt atgactgccc cctgacaagt ctgatgactt tatcaccgtc
       61 agcttctccc tggtacgcag caataatcag cccgccaatg tcagggtctc cccatctgtt
      121 gcggacagag ctcgccacga ttctgtaaat aatatcttct gtggttatat ttattttcac
      181 ccagtcagtc tggatatggg ccagtagcgg tagcgggtgt gataaatggg gccgttaatg
      241 ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg gcgtgcatca
      301 gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat gccgtagggg
      361 ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa atcactctta
      421 gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa tgcgccgggt
      481 atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat cgtggccgca
      541 cttttcagcc ccagattatc ccgggact
//
LOCUS       DAZPFP010000507          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPFP010000507 DAZPFP010000000
VERSION     DAZPFP010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.515"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>566
                     /gene="virK"
                     /locus_tag="ACHUR4_004446"
     CDS             <1..>566
                     /gene="virK"
                     /locus_tag="ACHUR4_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1457695.1"
                     /translation="FIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTFSVIL
                     TQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECLA
                     VSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASK
                     KRSGYRKRYALLDYIHETTIRSLDAYPV"
BASE COUNT          153 a          110 c          135 g          168 t
ORIGIN      
        1 attcatcggg aaagatggag agaattttga tatccagtgt tctcccagtg gttttgaccg
       61 ggaaggagag ttgatgttgt cattgtttta caataaaaca gttattgccc ggttaacatt
      121 ttcagtcatt ctgactcaaa atggacacat tgcttttatt ggcggattac agggagcccc
      181 taaaaatacc ggacctgatg ttattcggtg cgcaacccgg gcctgttatg ggattttccc
      241 aaaacggatt atttttgaag cattttgtgc gttgatgaaa gcttgcaata tctctgagtg
      301 tctggcagtc agtgagcaca gtcatgtttt ccggcaattg agatactggt atcagaaacg
      361 caaaaccttt gttgcggtct atagtgattt ctgggagtct gtagcaggaa aaacctgtgg
      421 tgactggtac agattaccaa cccaggtgat tcgaaagccg ctaagcgata tagccagtaa
      481 aaaacgctct gggtaccgaa aacgatacgc tttgctggat tatatccatg aaactactat
      541 ccgctctttg gatgcatatc ctgtac
//
LOCUS       DAZPFP010000508          562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPFP010000508 DAZPFP010000000
VERSION     DAZPFP010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.516"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..227)
                     /locus_tag="ACHUR4_004447"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="ACHUR4_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="ACHUR4_004448"
                     /pseudo
     CDS             211..469
                     /locus_tag="ACHUR4_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          138 a          154 c          124 g          146 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
      541 gactcttgtt ggattttacc aa
//
LOCUS       DAZPFP010000509          558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPFP010000509 DAZPFP010000000
VERSION     DAZPFP010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.517"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..276)
                     /locus_tag="ACHUR4_004449"
     CDS             complement(<1..276)
                     /locus_tag="ACHUR4_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1457696.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDE"
     gene            352..501
                     /locus_tag="ACHUR4_004450"
                     /pseudo
     CDS             352..501
                     /locus_tag="ACHUR4_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          160 a          108 c          117 g          173 t
ORIGIN      
        1 ttcatccgga aaagcaaagt gatgataatc cacgtgacca ctacttttgt taaactgtgc
       61 tatccagtag gaagctaaat gtcgacagac aataagtctt ccagagtgat cggtaacttg
      121 gcaatttaga tttaataaaa aactcctctt gtcagaaaaa tatggcaatt gttttgagtg
      181 caatagagac tggattgtct caacagagac gttgtcttta tttatcatat tttgcaaaca
      241 tattttcctt gatgggatta aatttactga tggcatatgt atatattcct ttcattgatg
      301 ggttgtgacg cagacttagt gttgttgtac tctttgccct ttggatgatt gatggcaaag
      361 attgatgtgg tttgtcaccg ttactctgaa actcaggggg gtatccggaa cggccattcc
      421 agttcagggg ggctgctcta tcgctgtaag cagtgcctga agaccttcca gttcgactac
      481 cgctacaacg gggcgaaacc catcaaactt tccaactatt atcacattga gccatactga
      541 caataaacaa tatatcga
//
LOCUS       DAZPFP010000510          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPFP010000510 DAZPFP010000000
VERSION     DAZPFP010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.518"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            136..459
                     /locus_tag="ACHUR4_004451"
     CDS             136..459
                     /locus_tag="ACHUR4_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1457697.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          126 a          136 c          124 g          171 t
ORIGIN      
        1 taatcaaaaa acagcccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc
       61 aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac
      121 tcttctggct ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg
      181 tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg
      241 ttggtttatg accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg
      301 atcgccgatc gcgcatgcca gcaggaaggc tatacccacg cgatcccctt tggtcagcca
      361 gtaggcaact gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca
      421 tatcagtgcc atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga
      481 tcttcgctga ttaaagaaat agcggtaatg cgttttattc ccattcgtat ttttaataat
      541 tgaagtttat attttac
//
LOCUS       DAZPFP010000511          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPFP010000511 DAZPFP010000000
VERSION     DAZPFP010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.519"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>551)
                     /locus_tag="ACHUR4_004452"
     CDS             complement(<1..>551)
                     /locus_tag="ACHUR4_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR1457698.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAZPFP010000512          543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPFP010000512 DAZPFP010000000
VERSION     DAZPFP010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.520"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(12..543)
                     /gene="ospZ"
                     /locus_tag="ACHUR4_004453"
                     /pseudo
     CDS             complement(12..543)
                     /gene="ospZ"
                     /locus_tag="ACHUR4_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT          166 a          111 c           80 g          186 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgc
//
LOCUS       DAZPFP010000513          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPFP010000513 DAZPFP010000000
VERSION     DAZPFP010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.521"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHUR4_004454"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHUR4_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZPFP010000514          535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPFP010000514 DAZPFP010000000
VERSION     DAZPFP010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.522"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="ACHUR4_004455"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="ACHUR4_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR1457699.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          145 a          125 c          159 g          106 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc cggaa
//
LOCUS       DAZPFP010000515          533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPFP010000515 DAZPFP010000000
VERSION     DAZPFP010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.523"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>533)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUR4_004456"
     CDS             complement(<1..>533)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUR4_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1457700.1"
                     /translation="RDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSY
                     NQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNI
                     SYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILN
                     LRNECSIHISDNPLSSH"
BASE COUNT          152 a           87 c          130 g          164 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gct
//
LOCUS       DAZPFP010000516          533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPFP010000516 DAZPFP010000000
VERSION     DAZPFP010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.524"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            382..>533
                     /locus_tag="ACHUR4_004457"
     CDS             382..>533
                     /locus_tag="ACHUR4_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1457701.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          131 a          141 c          115 g          146 t
ORIGIN      
        1 ctagggtctc tctataggtc ttctctatag gtcttctcta tggtctctct ataggtctct
       61 ctagggtctc ctctaggcat tacccccaga agctaactct atggccctct atggtcctct
      121 atggccctgt acgtcattct ttaggatgcc ctgtagtttc gtatagcctg atgcctagag
      181 agaccctaga gagaccctag agtggctcct agagagaccc tcccagtagc tccgcacagc
      241 tacatcgtca tcatcatagt attcaatctc aaagtcattc tgtttgcatc ctgttgtcct
      301 gctggtctcc cttcgcttgg ggactttgag taggtcagtg ggggtgcgac ttcagggtgc
      361 aaacaaaaag gaaatcaagc gatgtctcaa caaccacaac aaccacaaca agaaatcagc
      421 atcattaacc tcgaccagct tgtgtccatg acctcagttg aaattgctga gctgacaggc
      481 aaggagcacc gaaatgtcct tcgtgatatt cgtaagatgc tcgaagaact taa
//
LOCUS       DAZPFP010000517          524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPFP010000517 DAZPFP010000000
VERSION     DAZPFP010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.525"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            107..493
                     /locus_tag="ACHUR4_004458"
                     /pseudo
     CDS             107..493
                     /locus_tag="ACHUR4_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          169 a           77 c          104 g          174 t
ORIGIN      
        1 gatgattttt attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat
       61 catgatgatg tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca
      121 gcattctcgc tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt
      181 tcatacaacg gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt
      241 actgcttgat gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt
      301 caaaaaagta acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc
      361 ggctgttgag ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt
      421 tgattcccaa caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta
      481 ttatcggcgt aataaattat gggaattgga ggcaatgcac tgag
//
LOCUS       DAZPFP010000518          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPFP010000518 DAZPFP010000000
VERSION     DAZPFP010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.526"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..433)
                     /locus_tag="ACHUR4_004459"
     CDS             complement(<1..433)
                     /locus_tag="ACHUR4_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457702.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYI"
     gene            complement(426..>520)
                     /locus_tag="ACHUR4_004460"
     CDS             complement(426..>520)
                     /locus_tag="ACHUR4_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1457703.1"
                     /translation="LGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          169 a          105 c           82 g          164 t
ORIGIN      
        1 aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat
       61 cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc gaaactttgg
      121 ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa aaatacgaat
      181 gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac
      241 catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga tctgttttag
      301 catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca cgagccttcc
      361 agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata aaatacattt
      421 tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc attagaggat
      481 tttatacatc ttgataaata ccgctgccct tccccaagca
//
LOCUS       DAZPFP010000519          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPFP010000519 DAZPFP010000000
VERSION     DAZPFP010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.527"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>520)
                     /locus_tag="ACHUR4_004461"
     CDS             complement(<1..>520)
                     /locus_tag="ACHUR4_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1457704.1"
                     /translation="IKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGYFN
                     AGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNT
                     RYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDL
                     LKPVNSNQYRYCA"
BASE COUNT          162 a          103 c           82 g          173 t
ORIGIN      
        1 ttgcacaata ccgatactga ttgctattta caggtttaag taaatcttct ttactccacg
       61 gagaagctgc tttggcgatc aaaaaactac gtgaaaccgg ataatcagcc cactcatgcc
      121 agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact ggattatcaa
      181 ctttgtcttt taattcatag ttaatactat atcgggtatt atatttctcc gaaataaact
      241 tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg attttactta
      301 cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt agattccact
      361 catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaac tgtggtgtag
      421 ttaataccga ggctcgattt tgccaccatt caatatctct ttcagctaca actgcggcaa
      481 tttcattagt agaaaattga taatctaaga gttctttaat
//
LOCUS       DAZPFP010000520          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPFP010000520 DAZPFP010000000
VERSION     DAZPFP010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.528"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHUR4_004462"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHUR4_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZPFP010000521          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPFP010000521 DAZPFP010000000
VERSION     DAZPFP010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.529"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(47..442)
                     /locus_tag="ACHUR4_004463"
     CDS             complement(47..442)
                     /locus_tag="ACHUR4_004463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457705.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          148 a          122 c          108 g          131 t
ORIGIN      
        1 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcat
       61 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
      121 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      181 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      241 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      301 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      361 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      421 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      481 ctatccgtca tgcctgagat tgcgtcggt
//
LOCUS       DAZPFP010000522          506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPFP010000522 DAZPFP010000000
VERSION     DAZPFP010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.530"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT          165 a           77 c          101 g          163 t
ORIGIN      
        1 cgaatgttga cacctgagta tttcctgcta aaaatagccc tgctttacgg atggttgtat
       61 tgcaataaca cgaaagtagt aagagattaa aggtatctgt cagcgataac tctgcaagtg
      121 atgacatttt gtaaaccaaa taatgtaaat cagcaatgta tcttttcact taattataac
      181 aaatgtggta ttcactaaga atctgatacg agcttacatc acgactattt tagtctcatc
      241 gcgggataca gggatgcacc gatggatctc agcacaccgt aatgcaatac ctgaacaaat
      301 agaacagtaa agtggttcag tattaatgaa agctcttatt ttccgttggg aaaacttaaa
      361 atagatgaga aggctttttg atttcttatg tgatctcatt tcgagtattg agaaatttgt
      421 gatttttaaa tttttcaata agccctgtaa gggattgggg ttttatgaga aataaagaga
      481 cgtattgtct ttgtttaaac ggtacg
//
LOCUS       DAZPFP010000523          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPFP010000523 DAZPFP010000000
VERSION     DAZPFP010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.531"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>503
                     /locus_tag="ACHUR4_004464"
     CDS             <1..>503
                     /locus_tag="ACHUR4_004464"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR1457706.1"
                     /translation="KITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFC
                     SIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGND
                     VWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELL
                     RIKWWNY"
BASE COUNT          159 a           74 c          106 g          164 t
ORIGIN      
        1 caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa
       61 tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat
      121 aggtcgattt tgtagtattg catcaaatgt aaaaataatg ggtggaaatc atcctttgaa
      181 tcgttttact actcacatga tgacttataa cggtgaattt gataagtttg ctatgagtga
      241 atttgaacgt agctggacat tgaaaccatt cattaccaaa cctgaaaatc cgattatagg
      301 aaatgatgtc tggataggaa atgatgttgt attgaaaggt ggcatcgcaa taggtgatgg
      361 tgctgttatt gctgccaatt ccgttgttac caaagatgtg cccccatatg ccatagttgc
      421 aggagttcct gcaaaaataa tcagatttag gtttgattca aatgttattg atgaactgtt
      481 gagaataaaa tggtggaatt ata
//
LOCUS       DAZPFP010000524          501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPFP010000524 DAZPFP010000000
VERSION     DAZPFP010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.532"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUR4_004465"
     CDS             <1..228
                     /locus_tag="ACHUR4_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR1457707.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          167 a          104 c           81 g          149 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt t
//
LOCUS       DAZPFP010000525          498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPFP010000525 DAZPFP010000000
VERSION     DAZPFP010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.533"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..454
                     /gene="ipgB2"
                     /locus_tag="ACHUR4_004466"
     CDS             <1..454
                     /gene="ipgB2"
                     /locus_tag="ACHUR4_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR1457708.1"
                     /translation="SSTKINTSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKF
                     CAEHSRKIGDNLRKQIFKQVEKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGM
                     NRSVKNDTTSNVANLISDQFFEKNVQYIDLKKLRGNMSDYITNLESPF"
BASE COUNT          198 a           75 c           90 g          135 t
ORIGIN      
        1 atcatcaacc aaaataaata ctagtatttt gagctctgtt tcagaacaaa tcggtgaaaa
       61 tattacagat tggaaaaatg atgaaaaaaa agtctacgta tcccgggtgg taaaccaatg
      121 tattgataag ttttgtgcag aacacagccg aaaaatcggc gataatctcc gtaaacagat
      181 atttaaacag gttgagaaag attatcgcat ttctctagac atcaatgctg cacaaagttc
      241 cataaatcat cttgtatcag gaagcagtta tttcaaaaag aaaatggacg aactttgtga
      301 agggatgaat agaagcgtta aaaatgatac aacaagcaat gttgcaaatc ttatttccga
      361 tcagtttttt gagaaaaatg tgcaatatat tgatctaaag aaattacgtg ggaatatgag
      421 tgactatatt acaaatttag aatcgccttt ctgatgacag aacatcccga gtaaagaaac
      481 taagaaaagt aaataccc
//
LOCUS       DAZPFP010000526          497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPFP010000526 DAZPFP010000000
VERSION     DAZPFP010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.534"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHUR4_004467"
     CDS             complement(<1..224)
                     /locus_tag="ACHUR4_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR1457709.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQL"
BASE COUNT          128 a          126 c          114 g          129 t
ORIGIN      
        1 tttagttgtt tacgcgtaaa tcgcccggaa tccagaggat cattaagtat cacataaaca
       61 ggttccatac cagccctgtc cgcatcagga cgccagtaaa taaaatcttg tagtccaccg
      121 gcatcagaga caggagtaat cgtcgttacc ggcatttcga ctggttttat ttccgtacct
      181 gtatgtactg gtgtgagcgg acaaaaccac tgtacgtctc tcatacacgc tcgtaagcgg
      241 ccctcacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaaccc tcgtcgggac
      301 cactccgacc gcgcacaaaa gcattttcat cgccgatagc gggtatggct tggcagggcc
      361 gggatgggta taggtaattt ggctatcgta aaaaaggtgg ttaaaaatta caacctgcaa
      421 tgtaaattgg ctagttaatt tttggctatt taaattgact tcttaacggc gtttactgtt
      481 ccattgctcc ccaaccc
//
LOCUS       DAZPFP010000527          495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPFP010000527 DAZPFP010000000
VERSION     DAZPFP010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.535"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>495
                     /gene="mxiD"
                     /locus_tag="ACHUR4_004468"
     CDS             <1..>495
                     /gene="mxiD"
                     /locus_tag="ACHUR4_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1457710.1"
                     /translation="IDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFI
                     ASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLIN
                     VIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSL
                     LIGG"
BASE COUNT          161 a           71 c          109 g          154 t
ORIGIN      
        1 tattgataaa tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg
       61 tgattcgttt ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg
      121 caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc
      181 aaggccggtg attttgacac aagagaatat tccggctata tttgataata acaggacttt
      241 ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt
      301 gataaatgtt atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga
      361 ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct
      421 tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt
      481 gttgataggt ggtta
//
LOCUS       DAZPFP010000528          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPFP010000528 DAZPFP010000000
VERSION     DAZPFP010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.536"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..119)
                     /locus_tag="ACHUR4_004469"
                     /pseudo
     CDS             complement(1..119)
                     /locus_tag="ACHUR4_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          142 a           98 c          127 g          127 t
ORIGIN      
        1 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt caggttgtta
       61 cgttcaagat atagcatgtg ctggatgaag atcccttttc tggtcacctg tttatctttc
      121 gtggccgacg gggtgacact gttaaaatgt aatgaacgca tcccacacaa ttaaacacgg
      181 agtagtatga tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag ctttccggca
      241 tcatactgcc caatacggga tgcataaagc gtcctcagac tggagactcc acaaatgaaa
      301 tatacaccgg ttggcgttga tatggcaaaa catgtcattc agattcactt catcaatgag
      361 cacatgtcag cgccagtgat ataagacggt aatggggatg cggacgattt cctggactct
      421 cagaagatga aacatgttta ctgaagaaca aaaaattcgc gctattgagc tttatttcaa
      481 atatggtaag aagc
//
LOCUS       DAZPFP010000529          493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPFP010000529 DAZPFP010000000
VERSION     DAZPFP010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.537"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT          151 a           84 c          107 g          151 t
ORIGIN      
        1 ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc gactttaaaa
       61 ggatgggaat tacctgtact gatagtgaga aagctttcca ttgaaataag tcaaataaaa
      121 gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg aatcggaatt
      181 caatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat ctgaaaaact
      241 atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca gcacacgtta
      301 cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat tgcagccgtg
      361 atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg atttcatatt
      421 ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat cagcctcagg
      481 tatgaacacc aaa
//
LOCUS       DAZPFP010000530          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPFP010000530 DAZPFP010000000
VERSION     DAZPFP010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.538"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..240)
                     /gene="spaQ"
                     /locus_tag="ACHUR4_004470"
     CDS             complement(<1..240)
                     /gene="spaQ"
                     /locus_tag="ACHUR4_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR1457711.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMF"
     gene            complement(255..>489)
                     /gene="spa24"
                     /locus_tag="ACHUR4_004471"
                     /gene_synonym="spaP"
     CDS             complement(255..>489)
                     /gene="spa24"
                     /locus_tag="ACHUR4_004471"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR1457712.1"
                     /translation="PAYALSEIKDAFKIGFYLYLPFVVVDLVISSILLALGMMMMSPI
                     TISVPIKLVLFVALDGWGILSKALIEQYINIPA"
BASE COUNT          195 a           99 c           71 g          124 t
ORIGIN      
        1 aaacattatt tcatgacaaa aagacaataa aacctcacca taccatccag aaagaagtag
       61 caaagatatt gagacaccta taagctttat accaaaagga agtgtctgct cttggagttg
      121 agtcactgtc tgtaacaaac caatagataa tccaataacc gtagctatac ctactggcca
      181 taaggaaaag ataaggatta aataaagagc cttattaccc atataaacta tatcagacac
      241 attacctcca tcatctaagc aggaatattg atatattgtt caatcaaggc cttagacagg
      301 ataccccacc catcaagcgc aacaaataat acaagtttta ttggtactga tattgtgatt
      361 ggactcatca tcatcatacc aagtgcaagt aaaatgcttg aaataacaag atcaacaaca
      421 acaaatggca aatataaata aaaacctatt ttgaacgcat ctttgatttc acttagcgca
      481 tatgcaggc
//
LOCUS       DAZPFP010000531          487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPFP010000531 DAZPFP010000000
VERSION     DAZPFP010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.540"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>487
                     /locus_tag="ACHUR4_004472"
     CDS             <1..>487
                     /locus_tag="ACHUR4_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1457713.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAZPFP010000532          485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPFP010000532 DAZPFP010000000
VERSION     DAZPFP010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.541"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..485
                     /locus_tag="ACHUR4_004473"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACHUR4_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaaa
//
LOCUS       DAZPFP010000533          477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPFP010000533 DAZPFP010000000
VERSION     DAZPFP010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.542"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..129)
                     /locus_tag="ACHUR4_004474"
     CDS             complement(<1..129)
                     /locus_tag="ACHUR4_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457714.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVST"
     gene            complement(385..>477)
                     /locus_tag="ACHUR4_004475"
     CDS             complement(385..>477)
                     /locus_tag="ACHUR4_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR1457715.1"
                     /translation="NTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          141 a          100 c           95 g          141 t
ORIGIN      
        1 ggtggagaca atttctgcaa actttctttt taccgtccaa taatcaatat ttctattgtg
       61 attgttaacc actgcgtgca ttggcgggat tgaagggtga aaaatatgtg caattgggaa
      121 gcctttcatt gatgcctgcc ttgtcgtcat gacaaacaat aatagatgct tattttcctc
      181 gcacgacact tacgcactat caaaaaacgc tcatttaccc ggcgttacag taaacgaagc
      241 aacattctgt gtcctacgct gatgtaaagc acagtgaaaa tcccccttgt tgacttcgtc
      301 tgaccttatc gcggcgacca gcaattattc gttttgtgat cctcaacaca aaatgctatt
      361 cgtcgcctat gaggggagaa gaatttatgg aaaacgattg cattcacaaa atgacgtcct
      421 gtcagaagag aaaaagttga cgttaatcaa ttgttcgaga tgatcaatac ctgtatt
//
LOCUS       DAZPFP010000534          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPFP010000534 DAZPFP010000000
VERSION     DAZPFP010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.543"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>475)
                     /locus_tag="ACHUR4_004476"
     CDS             complement(<1..>475)
                     /locus_tag="ACHUR4_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1457716.1"
                     /translation="VTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVL
                     GFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMI
                     HLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNI"
BASE COUNT          155 a           95 c           72 g          153 t
ORIGIN      
        1 cttatgtttt gctttattaa tgccacgtca ttaaaatgac tatatttaat gaaacaacca
       61 tcattgagta gtgttttaag attgactaaa ttacttgcat cttcacgtac catcgtggca
      121 actggaacag gcagagacat gaaaacttct tcattcacca tcaagtgaat catgcattgc
      181 gttccctttg tcatatccac acttacgcta caaatatcgt ggtaatgcga agagtgcatt
      241 tcaggaaaca gattaaacac ggtatcttga aaggcactaa atcgtgttaa taaatcctga
      301 taatgaacag gttttgtttc aaaattatca tcagcaaatc caagcactat tgcatgctct
      361 acatctaacg cagtttcaaa tgactcttct tctgtaaaat cattatttaa aacctcacct
      421 gacgtattat cactgtcata aaaagtgctc atcaatgcga tattgtttgt tacag
//
LOCUS       DAZPFP010000535          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPFP010000535 DAZPFP010000000
VERSION     DAZPFP010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.544"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHUR4_004477"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHUR4_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZPFP010000536          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPFP010000536 DAZPFP010000000
VERSION     DAZPFP010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.545"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHUR4_004478"
     CDS             3..>472
                     /locus_tag="ACHUR4_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1457717.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZPFP010000537          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPFP010000537 DAZPFP010000000
VERSION     DAZPFP010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.546"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUR4_004479"
     CDS             <1..139
                     /locus_tag="ACHUR4_004479"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457718.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..471
                     /locus_tag="ACHUR4_004480"
                     /pseudo
     CDS             172..471
                     /locus_tag="ACHUR4_004480"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          104 c          115 g          113 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt g
//
LOCUS       DAZPFP010000538          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPFP010000538 DAZPFP010000000
VERSION     DAZPFP010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.547"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUR4_004481"
     CDS             <1..>465
                     /locus_tag="ACHUR4_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1457719.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZPFP010000539          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPFP010000539 DAZPFP010000000
VERSION     DAZPFP010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.548"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..465
                     /locus_tag="ACHUR4_004482"
                     /pseudo
     CDS             1..465
                     /locus_tag="ACHUR4_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          111 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaa
//
LOCUS       DAZPFP010000540          462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPFP010000540 DAZPFP010000000
VERSION     DAZPFP010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.549"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>462)
                     /gene="rfbG"
                     /locus_tag="ACHUR4_004483"
     CDS             complement(<1..>462)
                     /gene="rfbG"
                     /locus_tag="ACHUR4_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR1457720.1"
                     /translation="VIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWK"
BASE COUNT          140 a           96 c           60 g          166 t
ORIGIN      
        1 acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat
       61 tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt cgataccact
      121 tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt
      181 ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat
      241 ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat
      301 ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg
      361 tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat
      421 caactaaatt ttttcgccct gatggataat atgcaattac at
//
LOCUS       DAZPFP010000541          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPFP010000541 DAZPFP010000000
VERSION     DAZPFP010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.550"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUR4_004484"
     CDS             <1..>461
                     /locus_tag="ACHUR4_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457721.1"
                     /translation="LSPSCNEYYLKVWSEWEKNGTPGEQRNIAFNRLKICLQNQGAEL
                     NLSELDLKTLPDLPPQITTLEIRKNLLTYLPDFPPMLKVIHAQFNQLESLPALPETLE
                     ELNVGDNKIKELPYLPETLTHLRIHNNRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          165 a           92 c           79 g          125 t
ORIGIN      
        1 ttgagtccat cttgtaatga atattattta aaagtctgga gtgaatggga aaagaacggt
       61 accccaggcg agcaacgcaa tatcgccttc aataggctaa aaatatgttt gcaaaatcaa
      121 ggggcggaat taaatctatc tgagttagat ttaaaaacat tacctgattt accgcctcag
      181 ataacaacac tggaaataag aaaaaaccta ttaacatatc tccctgattt cccaccaatg
      241 cttaaggtaa tacatgctca atttaatcaa ctggaaagct tacctgcctt acccgagacg
      301 ttagaagagc ttaatgtggg tgataacaag ataaaagaat taccatatct tcctgaaact
      361 ctaactcatt tacggatcca taataaccga ttgcatattc tgccactatt gccaccggag
      421 ctaaaattac tgatagtttc tggaaacaga ttagacagca t
//
LOCUS       DAZPFP010000542          460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPFP010000542 DAZPFP010000000
VERSION     DAZPFP010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.551"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>460)
                     /locus_tag="ACHUR4_004485"
     CDS             complement(<1..>460)
                     /locus_tag="ACHUR4_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR1457722.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          147 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaat
//
LOCUS       DAZPFP010000543          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPFP010000543 DAZPFP010000000
VERSION     DAZPFP010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.552"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHUR4_004486"
     CDS             <1..132
                     /locus_tag="ACHUR4_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1457723.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEQLARIRWGG"
     gene            307..459
                     /locus_tag="ACHUR4_004487"
                     /pseudo
     CDS             307..459
                     /locus_tag="ACHUR4_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065945178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           90 c          138 g          109 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agcagctggc tcgaatacgg
      121 tggggcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc gcgcatccac
      361 ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa acaaccgggt
      421 gaggtggtgg ataaaacccg ccagaatgaa ccgccccgg
//
LOCUS       DAZPFP010000544          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPFP010000544 DAZPFP010000000
VERSION     DAZPFP010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.553"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            236..>459
                     /gene="ydeI"
                     /locus_tag="ACHUR4_004488"
     CDS             236..>459
                     /gene="ydeI"
                     /locus_tag="ACHUR4_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1457724.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT          146 a           87 c           85 g          141 t
ORIGIN      
        1 ttttctgtat gctcgtccaa tataataatt ttcaaacata taaatccgat tattctgaaa
       61 acatgtttaa gactcttcaa tcttcttaac tttagtgagt caaattctct gaatatttga
      121 cagctagtgg gctgatttta aatgccttat aacaattggc agtattctta tttggcaatt
      181 ataaaaatag caaccagact taataagtca aaccaaacgc aattaaggag ttagtatgaa
      241 atttcagtcc attgtattag caagttttct tgtcatgcct tatgcgttgg cagatgacca
      301 gggcggttta aaacaggatg cagcgccacc gccgcctcat gcaatagaag atggctatcg
      361 cggaaccgat gatgcaaaaa aaatgaccgt tgatttcgca aaaaacatgc acgatggcgc
      421 ttcggtttca ctacgcggta atttgatttc tcacaaagg
//
LOCUS       DAZPFP010000545          452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPFP010000545 DAZPFP010000000
VERSION     DAZPFP010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.554"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>452
                     /gene="gtrII"
                     /locus_tag="ACHUR4_004489"
     CDS             <1..>452
                     /gene="gtrII"
                     /locus_tag="ACHUR4_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR1457725.1"
                     /translation="LKLESYELLIGMLVFITFPQISYQLEFLNQADTVGIAFLLAAIS
                     AIIFHSQKNRIVIFSGIVLSILSMAIYQTFVTYIIAFVIGLQINSIIRNEKNIRESFY
                     SSCLSLSLIALSTLIYLLLTKAIKHYFSLESNEYISNYIQNASDIKWLV"
BASE COUNT          141 a           80 c           65 g          166 t
ORIGIN      
        1 ctaaagctag aatcttatga attattgata ggtatgttag tatttattac cttccctcag
       61 atctcctatc aattagagtt tcttaaccaa gctgatactg tgggaattgc ttttctactg
      121 gcagcgatat cagcaattat ttttcactcg caaaaaaata ggattgtgat attttctggt
      181 atagtactgt ccattctttc aatggcaatc taccaaacat tcgtaacata tattattgca
      241 ttcgtcattg ggttgcagat aaattcgata atacgaaatg agaaaaatat tcgtgaatct
      301 ttttatagtt catgtttatc tctatccctc atagctttat ctaccttaat ttacctgcta
      361 ttaaccaaag ctatcaagca ttatttttcg cttgaatcga acgagtacat ctcaaattat
      421 atacaaaatg caagcgatat taaatggctt gt
//
LOCUS       DAZPFP010000546          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPFP010000546 DAZPFP010000000
VERSION     DAZPFP010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.555"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHUR4_004490"
     CDS             213..>443
                     /locus_tag="ACHUR4_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1457726.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZPFP010000547          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPFP010000547 DAZPFP010000000
VERSION     DAZPFP010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.556"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>443
                     /gene="ospB"
                     /locus_tag="ACHUR4_004491"
     CDS             <1..>443
                     /gene="ospB"
                     /locus_tag="ACHUR4_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1457727.1"
                     /translation="FSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELIDV
                     QTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKESL
                     LEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSI"
BASE COUNT          143 a           78 c           78 g          144 t
ORIGIN      
        1 tttttctgta aaaaaagcga aaataaaaac agtaaacgat actgatttta aagagtataa
       61 taaggtttat attcttgggc acggtagtcc tggttctcat caattaggcc ttggttcgga
      121 acttattgat gtacaaacaa tcatttcaag aatgaaagac tgtggtattc taaatgtgaa
      181 agatatccgt tttacttcat gcggctccgc tgataaagtg gctcctaaaa attttaacaa
      241 tgcccctgct gaaagtcttt cttgtatcct taactctctg ccttttttta aggaaaaaga
      301 atctttgcta gagcagataa aaaaacacct tgaaaacgat gagtcattga gtgatggtct
      361 aaaaatatcc ggctatcatg gatatggagt tcactatggt caagagcttt ttccctactc
      421 acattatcgt tcaacttcaa ttc
//
LOCUS       DAZPFP010000548          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZPFP010000548 DAZPFP010000000
VERSION     DAZPFP010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.557"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>441)
                     /locus_tag="ACHUR4_004492"
     CDS             complement(<1..>441)
                     /locus_tag="ACHUR4_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR1457728.1"
                     /translation="NAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSG
                     NRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAF
                     VNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT          126 a           77 c          100 g          138 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattt gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat t
//
LOCUS       DAZPFP010000549          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPFP010000549 DAZPFP010000000
VERSION     DAZPFP010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.558"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..381)
                     /gene="mxiC"
                     /locus_tag="ACHUR4_004493"
     CDS             complement(<1..381)
                     /gene="mxiC"
                     /locus_tag="ACHUR4_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1457729.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRE"
BASE COUNT          130 a           93 c           56 g          157 t
ORIGIN      
        1 ttcccttaat gctaagactt gatcacttgg atctttaaaa tatctcttta gtctgtctat
       61 aaaatcccga tctagaggaa ggttatcttt taacgttctc tttaaatcaa aaatcttgtg
      121 attaatttca tcttcttccc catctaaaat acgttcctga ctatcactat ttgttccttt
      181 cagtttctca aggtctcttc tgtttataaa tgacgaaaga gcggaagcca tctcatcaga
      241 tgagtcaata tacttgctat tagccaagcc agaatcaagc tctgcatcag cagtctcatc
      301 tccatcatct gaatttgtca ttgcattcaa cttatcaatg aatgcagagc taaaaactcc
      361 tgtattttta acatcaagca tacatcacct actttatagt tagtaattta agtatgaaac
      421 caatgatttt tcgtct
//
LOCUS       DAZPFP010000550          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPFP010000550 DAZPFP010000000
VERSION     DAZPFP010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.559"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHUR4_004494"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHUR4_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZPFP010000551          430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPFP010000551 DAZPFP010000000
VERSION     DAZPFP010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.560"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT          130 a           88 c           81 g          131 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat
       61 ttcgtcagca tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt
      121 tcagccgcaa gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag
      181 taaacatatc aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac
      241 ggtcaatata gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt
      301 gctattctcc ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct
      361 ccgttactat ctgaaatgca atgagataat aaacgattac tctcggttag tatctcatga
      421 tcatttacag
//
LOCUS       DAZPFP010000552          417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPFP010000552 DAZPFP010000000
VERSION     DAZPFP010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.561"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHUR4_004495"
     CDS             <1..269
                     /locus_tag="ACHUR4_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1457730.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>417
                     /gene="tnpB"
                     /locus_tag="ACHUR4_004496"
     CDS             266..>417
                     /gene="tnpB"
                     /locus_tag="ACHUR4_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1457731.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSG"
BASE COUNT          100 a          112 c          119 g           86 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtgg
//
LOCUS       DAZPFP010000553          417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZPFP010000553 DAZPFP010000000
VERSION     DAZPFP010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.562"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>417)
                     /locus_tag="ACHUR4_004497"
     CDS             complement(<1..>417)
                     /locus_tag="ACHUR4_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1457732.1"
                     /translation="ENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYK
                     QCGVINPQNANTAYFGDTDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSA
                     YIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITP"
BASE COUNT          137 a           84 c           62 g          134 t
ORIGIN      
        1 caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg
       61 tctctcgtat ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa
      121 gccatgtctt cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct
      181 atatgccctg aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca
      241 aaataagccg tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt
      301 ttagaaaaca cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag
      361 acatcccaac gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttca
//
LOCUS       DAZPFP010000554          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZPFP010000554 DAZPFP010000000
VERSION     DAZPFP010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.563"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHUR4_004498"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHUR4_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1457733.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZPFP010000555          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZPFP010000555 DAZPFP010000000
VERSION     DAZPFP010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.564"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHUR4_004499"
     CDS             complement(<1..80)
                     /locus_tag="ACHUR4_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457734.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKAS"
     gene            98..289
                     /locus_tag="ACHUR4_004500"
     CDS             98..289
                     /locus_tag="ACHUR4_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1457735.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          101 a          106 c           81 g          128 t
ORIGIN      
        1 gacgcttttt tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta
       61 ccgtccacag cgtgggacat acttcaagga accttttgtg agtcaggcaa ctagtatgcg
      121 aaaacgacac cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt
      181 cttctttggc cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa
      241 agaagctcag caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc
      301 tccactttca cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat
      361 caaagcggca gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtt
//
LOCUS       DAZPFP010000556          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZPFP010000556 DAZPFP010000000
VERSION     DAZPFP010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.565"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            155..340
                     /locus_tag="ACHUR4_004501"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHUR4_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZPFP010000557          400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZPFP010000557 DAZPFP010000000
VERSION     DAZPFP010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.566"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>400)
                     /gene="spa29"
                     /locus_tag="ACHUR4_004502"
                     /gene_synonym="spaR"
     CDS             complement(<1..>400)
                     /gene="spa29"
                     /locus_tag="ACHUR4_004502"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR1457736.1"
                     /translation="SGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIF
                     HAVGSIIDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLS
                     YNICPLFSQCSFRVSNILTFLTLLASQAVIL"
BASE COUNT          171 a           72 c           69 g           88 t
ORIGIN      
        1 taaaataaca gcctgacttg ccaataaagt cagaaatgtt aagatatttg agacacggaa
       61 agaacattga gaaaataacg ggcatatatt ataagacaat tgtatggatt ctaaaataaa
      121 gaccatacca ccactgtata gaaatacaac tgcagaaaaa agattgaaaa attttgccaa
      181 ctcagacgta tcaacaccat tggcaggatc aattgaacta ctaagcgttg ccccacgctg
      241 gttgtcaata atgctaccaa cagcatgaaa tatccaaaag ggaagagaca agcaaaaaga
      301 gagaaggagg ccaacaatta tttccttgaa cataaggaaa taaacatgtt caaaaacaga
      361 agaacctatg tctaccttac cagaagtaat taatcctgat
//
LOCUS       DAZPFP010000558          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZPFP010000558 DAZPFP010000000
VERSION     DAZPFP010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.567"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT          100 a           86 c           79 g          130 t
ORIGIN      
        1 aacagatttt aatatgttgt atggttttca ctctgaaatc ccctgaattt aatcacgttt
       61 cctttactga aggcaatttt aaccgtagta tcaattatta acccttgggc ttgatatccc
      121 tctcctcttc tgggcacggg ggatgtcaga ttacttatta cagaaaccaa aagaattgta
      181 agcgtcatct cagtgtcgtc tatttcaatg tctgatgatt ggcgatgata gagtcctctt
      241 ataacgttaa tggactctat caatgtcaga ttctcttcgc ccccgcagga ttcgtgcctc
      301 ttactctatg actctatgga gtttaagctt gatcttattg aaaaatctta ccagctaggg
      361 gcctgcgtgg cccaactggc cagagagtac gggat
//
LOCUS       DAZPFP010000559          392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZPFP010000559 DAZPFP010000000
VERSION     DAZPFP010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.568"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..286)
                     /locus_tag="ACHUR4_004503"
     CDS             complement(<1..286)
                     /locus_tag="ACHUR4_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1457737.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVALKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSAS"
BASE COUNT           98 a           91 c           78 g          125 t
ORIGIN      
        1 cactggcgga acctttggtc atatccagtg cgccattttg caacaattcc atagtttctc
       61 gcgcattacc catctggctg ctgggataaa tacggatcac catattacct tttgacagct
      121 gttttacctc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
      181 tgtgtgctaa ctttaacgcc actttttctg cggcgtttac gccagtgcta accagagcca
      241 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
      301 ttgaaggatg gaaattggtt gatatctaat tgtgcttatc agtgatttgt gcgacaaatt
      361 ggttaacctg tctgacaact tgtctatgtt ta
//
LOCUS       DAZPFP010000560          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZPFP010000560 DAZPFP010000000
VERSION     DAZPFP010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.569"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHUR4_004504"
     CDS             complement(42..>390)
                     /locus_tag="ACHUR4_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1457738.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZPFP010000561          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZPFP010000561 DAZPFP010000000
VERSION     DAZPFP010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.570"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..206
                     /gene="ipgF"
                     /locus_tag="ACHUR4_004505"
     CDS             <1..206
                     /gene="ipgF"
                     /locus_tag="ACHUR4_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1457739.1"
                     /translation="HPCLSVHYAAKLLNEFMMMYGRGWEAVGAYNAGTSPKKKKERLK
                     YAEDIYRRYLRIAAESKQNNRRI"
     gene            215..>390
                     /locus_tag="ACHUR4_004506"
     CDS             215..>390
                     /locus_tag="ACHUR4_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR1457740.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVT"
BASE COUNT          134 a           58 c           94 g          104 t
ORIGIN      
        1 gtcatccatg tctttctgta cattatgcag caaagttatt aaatgagttt atgatgatgt
       61 atggaagagg gtgggaggct gttggagcct ataatgcagg aacgtctccg aaaaagaaaa
      121 aagaaagatt aaagtacgca gaggatatct atagacgcta tttgcggata gcagcagaga
      181 gcaagcagaa taatcgaagg atataagagg atttatgtct gaggcaaaga actcaaatct
      241 tgcaccattc aggttattag tgaaactcac aaacggtgtc ggggatgagt ttccgttata
      301 ttatgggaat aacttaattg ttctcggacg gacgatagaa acacttgagt ttggaaacga
      361 caatttccca gaaaatatta ttcctgtaac
//
LOCUS       DAZPFP010000562          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZPFP010000562 DAZPFP010000000
VERSION     DAZPFP010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.571"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUR4_004507"
     CDS             complement(<1..209)
                     /locus_tag="ACHUR4_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457741.1"
                     /translation="MIHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSW
                     KSDFAWTGIMLLFRGEELAFSSAFSP"
BASE COUNT          129 a           93 c           79 g           83 t
ORIGIN      
        1 ggtgagaagg cggaagaaaa agccagttct tcgcccctaa ataacagcat gattccagtc
       61 catgcaaaat ctgatttcca gctcacacat accgagcgct gtacaaaaaa ccgccagcca
      121 aggcagttaa gagtgtgttc ccggggtttg cttagaatat tttgtaagtt gtccgaagag
      181 atatttacaa caccagaatg atgaatcatc agtcccctgc cagaggttca ctacgtaacc
      241 tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac tggaactgca acaaaacaac
      301 atcacagaac aggagatggt aaggagtcag acattcccac acaatattgt gtcagtgcaa
      361 ataacaacct tcgtctcaaa tctc
//
LOCUS       DAZPFP010000563          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZPFP010000563 DAZPFP010000000
VERSION     DAZPFP010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.572"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>380)
                     /locus_tag="ACHUR4_004508"
     CDS             complement(<1..>380)
                     /locus_tag="ACHUR4_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1457742.1"
                     /translation="CNFLMDKDAQGYIDLSDFDLTNCHFKGDVISKVSFLSSNLQHVT
                     FECKEIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFSQNILDTVDFSQSQLGH
                     SNFRECQIRNSNFDNCYLYASHFT"
BASE COUNT          138 a           68 c           58 g          116 t
ORIGIN      
        1 ctggtgaagt gcgaagcgta aagataacaa ttatcgaagt ttgaatttct aatctgacat
       61 tcgcgaaaat tactatgacc aagttgactc tgcgagaagt caactgtatc aagaatattt
      121 tggctaaaat caacacattc gccactcgtt ttgagaaaaa tcacattgtg taaacgtcga
      181 catttaaata tgacattatc aactgttgcc ttagtaaaat tgcaattctc aatttcttta
      241 cattcgaaag ttacatgttg tagatttgat gataaaaaag acaccttaga aataacgtca
      301 cctttaaaat gacaatttgt taaatcaaaa tcagacaggt cgatataccc ctgcgcatct
      361 ttatccatca gaaaattaca
//
LOCUS       DAZPFP010000564          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZPFP010000564 DAZPFP010000000
VERSION     DAZPFP010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.573"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUR4_004509"
     CDS             <1..>375
                     /locus_tag="ACHUR4_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1457743.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZPFP010000565          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZPFP010000565 DAZPFP010000000
VERSION     DAZPFP010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.574"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUR4_004510"
     CDS             <1..>375
                     /locus_tag="ACHUR4_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1457744.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          114 a           72 c          116 g           73 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      361 aaaagagcca gtcag
//
LOCUS       DAZPFP010000566          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZPFP010000566 DAZPFP010000000
VERSION     DAZPFP010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.575"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUR4_004511"
     CDS             <1..>374
                     /locus_tag="ACHUR4_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR1457745.1"
                     /translation="RHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLV"
BASE COUNT          117 a           48 c           87 g          122 t
ORIGIN      
        1 cgacatgtga ctatacagag aatttctgag gtattacaaa gattgcttgg agaaaatatt
       61 tctgttcgca atttaaaact tattatggag tctttggcgc tttgggctcc cagagaaaaa
      121 gatgtcataa cattagttga acatgtccgt gcatcacttt ctaggtatat ttgtagtaaa
      181 atagctgttt ctggtgagat taaagttgtg atgctttccg gatatattga ggatgcaata
      241 agaaaaggga taaggcaaac ctctggtggc tctttcttga atatggatat agaggtttcg
      301 gatgaggtaa tggaaacttt agcacatgct ttgagagaat tgagaaatgc aaaaaaaaat
      361 ttcgttcttt tggt
//
LOCUS       DAZPFP010000567          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZPFP010000567 DAZPFP010000000
VERSION     DAZPFP010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.576"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUR4_004512"
     CDS             <1..>374
                     /locus_tag="ACHUR4_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1457746.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZPFP010000568          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZPFP010000568 DAZPFP010000000
VERSION     DAZPFP010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.577"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUR4_004513"
     CDS             complement(<1..311)
                     /locus_tag="ACHUR4_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1457747.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZPFP010000569          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZPFP010000569 DAZPFP010000000
VERSION     DAZPFP010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.578"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUR4_004514"
     CDS             <1..>365
                     /locus_tag="ACHUR4_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR1457748.1"
                     /translation="KQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAAL
                     DEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRF
                     QPQEHVYWLHKIGNLTLIS"
BASE COUNT          132 a           58 c           68 g          107 t
ORIGIN      
        1 aaaacaaatt aaaagccaaa gagaaatggt ttgttattct gctctggtag caataaataa
       61 tgatatgcct tttgattctg tgataaatca gataaaccaa catgcagata attcaggttt
      121 tatagctgct ctggatgaag atttatatga gcctcgtccg aatcaagtga acttaataaa
      181 agcaattttg cttcgcttag atatggagca acaagatgag agcgtgatta aaacctatac
      241 ggggagaatc acaattgagc atattcttcc tcaagcatta gttaatgaat actggattaa
      301 tagattccaa cctcaagagc atgtctactg gctgcataaa ataggtaatt taacactcat
      361 aagtg
//
LOCUS       DAZPFP010000570          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZPFP010000570 DAZPFP010000000
VERSION     DAZPFP010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.579"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUR4_004515"
     CDS             <1..>365
                     /locus_tag="ACHUR4_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1457749.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZPFP010000571          355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZPFP010000571 DAZPFP010000000
VERSION     DAZPFP010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.580"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>355
                     /locus_tag="ACHUR4_004516"
     CDS             <1..>355
                     /locus_tag="ACHUR4_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457750.1"
                     /translation="DTKALQKNISCVTNSSSSGISEKHLDHCADTVKSFLRKSIAAQS
                     YSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERH
                     LLSIIINSDFNFRHQ"
BASE COUNT          120 a           73 c           54 g          108 t
ORIGIN      
        1 aagatacaaa agcattacaa aaaaacatat catgcgtaac aaactcatcc tcttctggca
       61 taagtgaaaa acatttagat cactgtgctg atactgtgaa aagcttttta aggaagtcta
      121 tagcagcgca aagttatagt aaaatgttct ctcaagggac cagtttcaaa tcattaaatc
      181 tttctattga agcgccatct ggagcacggt catcatttcg gtcacttgag cacctcgata
      241 aggtgtccag acactatctt tctgaaataa tacaaaaaac tcatcctctt tcttcggatg
      301 aacgtcactt gctctctatt attattaatt ctgattttaa cttcagacat caaag
//
LOCUS       DAZPFP010000572          355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZPFP010000572 DAZPFP010000000
VERSION     DAZPFP010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.581"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            43..>355
                     /gene="virB"
                     /locus_tag="ACHUR4_004517"
     CDS             43..>355
                     /gene="virB"
                     /locus_tag="ACHUR4_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1457751.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     AD"
BASE COUNT          135 a           60 c           68 g           92 t
ORIGIN      
        1 acttttgtca atataaatca atatgaataa acagggtgtg atatggtgga tttgtgcaac
       61 gacttgttaa gtataaagga aggccaaaag aaagagttta cactccattc tggtaataaa
      121 gtttccttta tcaaagccaa gattcctcat aaaaggatcc aagatttaac cttcgtcaac
      181 caaaaaacga atgtacgcga tcaagaatcc ctaacagaag aatcattagc cgatatcata
      241 aaaactataa agctacaaca attcttccct gtaataggaa gggagattga tggtagaatt
      301 gaaattctgg atggcactcg tagaagagca tctgcaatat atgcaggagc agatc
//
LOCUS       DAZPFP010000573          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZPFP010000573 DAZPFP010000000
VERSION     DAZPFP010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.582"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHUR4_004518"
     CDS             <1..264
                     /locus_tag="ACHUR4_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1457752.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="ACHUR4_004519"
     CDS             288..>353
                     /locus_tag="ACHUR4_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1457753.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAZPFP010000574          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZPFP010000574 DAZPFP010000000
VERSION     DAZPFP010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.583"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           96 a           63 c           77 g          112 t
ORIGIN      
        1 cataggatgt agatggagca gtccgagatt ctactaatat caattattaa gcatctgttt
       61 tttagttggt gtaatataat ttttattaag gtaagcggaa aaacaaccaa aaaaaacgca
      121 agatgttatt tttttatcat tttgagccag tttggctggg gggatactct tctcttaagc
      181 ttcttgctca actttaaaaa tagttatgcc agttcggtat cgataagggg cagtttggat
      241 atcgagaacc acggcagcct gacggctgca aattgaaaac tgcccctgtt cgtcctggtg
      301 ttgccgcacg ctccgcagaa tatgtatgct ctgaggacct gttttatt
//
LOCUS       DAZPFP010000575          347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZPFP010000575 DAZPFP010000000
VERSION     DAZPFP010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.584"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>347
                     /gene="ygeQ"
                     /locus_tag="ACHUR4_004520"
     CDS             <1..>347
                     /gene="ygeQ"
                     /locus_tag="ACHUR4_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR1457754.1"
                     /translation="HSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEKPWGTFILA
                     STFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYMSTPDQIKR
                     MQDIISNPKRLDG"
BASE COUNT          122 a           71 c           78 g           76 t
ORIGIN      
        1 cactcagcca aattaaaaaa tgaaataaag agtcactata ccaaagacga aactcaagga
       61 ttgctaacat taacagaaaa cacacgaaag ttaacgttga cggagaaacc ctggggaacg
      121 tttattcttg cttctacttt cgaagatgac aaaacagcgg cagaaaccca ctatgatgct
      181 gtctggctga gggatagttt atggggctat atggcattag tatcagacca aggaaatagt
      241 gtagccgcaa aaaaggttct gctcaccctg tggggttata tgtctacgcc cgaccagatt
      301 aagcggatgc aggacatcat tagcaaccca aaacgactgg atggaat
//
LOCUS       DAZPFP010000576          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZPFP010000576 DAZPFP010000000
VERSION     DAZPFP010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.585"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            1..345
                     /locus_tag="ACHUR4_004521"
                     /pseudo
     CDS             1..345
                     /locus_tag="ACHUR4_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           74 a           91 c          105 g           75 t
ORIGIN      
        1 tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc
       61 aaccgcccgc attatgggcg ttggcctcaa cacgattttc cgccatttaa aaaactcagg
      121 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
      181 gacgaacagt ggggatacgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
      241 gacaggctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
      301 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatgg
//
LOCUS       DAZPFP010000577          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZPFP010000577 DAZPFP010000000
VERSION     DAZPFP010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.586"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHUR4_004522"
     CDS             <1..>344
                     /locus_tag="ACHUR4_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1457755.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZPFP010000578          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZPFP010000578 DAZPFP010000000
VERSION     DAZPFP010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.587"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>342
                     /locus_tag="ACHUR4_004523"
     CDS             <1..>342
                     /locus_tag="ACHUR4_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457756.1"
                     /translation="NINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKSVNNG
                     EERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADNAS
                     LVNINLSVNKI"
BASE COUNT          121 a           64 c           57 g          100 t
ORIGIN      
        1 ccaatattaa tacagcttgt gtaaaaaata atgccagtta tcaacttaat aacgcattac
       61 ctaacaaaga aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataaat
      121 cggttaataa tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt
      181 cgaacatggc atctcttgat ttgtcatatc ttgatttgag tgaattaccg cctatcccct
      241 cgacagttaa tacgttaaat ctggaaaaca attgtctaac ttgtcttgac ttagctgaca
      301 atgccagtct cgttaatatc aacctcagcg ttaataaaat ta
//
LOCUS       DAZPFP010000579          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZPFP010000579 DAZPFP010000000
VERSION     DAZPFP010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.588"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            2..172
                     /gene="xisR"
                     /locus_tag="ACHUR4_004524"
     CDS             2..172
                     /gene="xisR"
                     /locus_tag="ACHUR4_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1457757.1"
                     /translation="MMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            174..>342
                     /locus_tag="ACHUR4_004525"
     CDS             174..>342
                     /locus_tag="ACHUR4_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1457758.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           65 c           83 g           86 t
ORIGIN      
        1 cctgatgctt cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg
       61 ggtggaaggc ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg
      121 gattatctgg tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta
      181 aattaccaac aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag
      241 gaaaacgatg tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg
      301 ccgggaattt aagagcgttg atagttcatc aaatcagttc cg
//
LOCUS       DAZPFP010000580          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZPFP010000580 DAZPFP010000000
VERSION     DAZPFP010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.589"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>340)
                     /gene="spa33"
                     /locus_tag="ACHUR4_004526"
                     /gene_synonym="spaO"
     CDS             complement(<1..>340)
                     /gene="spa33"
                     /locus_tag="ACHUR4_004526"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR1457759.1"
                     /translation="ELSDKITFRITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQ
                     VSLDSLHHLLEFCLGSSNVRLATLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNN
                     EAKINLSESNG"
BASE COUNT          129 a           62 c           46 g          103 t
ORIGIN      
        1 accattactt tctgacagat taatttttgc ctcattatta tcattcacaa tataatcccc
       61 aataataact tgattacaca ataataaatt ataaagtttc tgaactatga ttatatcacc
      121 agtgcgaatt ctttttaaag tagccagcct tacattagat gaacctaagc aaaactctaa
      181 aagatgatgc aatgagtcaa gagaaacttg attacgtaat accggcatct tgtcaaaata
      241 aataatacct aaagaagagg aaaaacagca taaatgactc ccagtagttg catactggat
      301 ttcattcgtg atccggaaag taatcttatc agacagttct
//
LOCUS       DAZPFP010000581          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZPFP010000581 DAZPFP010000000
VERSION     DAZPFP010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.590"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUR4_004527"
     CDS             <1..>338
                     /locus_tag="ACHUR4_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1457760.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAZPFP010000582          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZPFP010000582 DAZPFP010000000
VERSION     DAZPFP010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.591"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHUR4_004528"
     CDS             complement(43..330)
                     /locus_tag="ACHUR4_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457761.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAZPFP010000583          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZPFP010000583 DAZPFP010000000
VERSION     DAZPFP010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.592"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHUR4_004529"
     CDS             51..>332
                     /locus_tag="ACHUR4_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1457762.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZPFP010000584          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZPFP010000584 DAZPFP010000000
VERSION     DAZPFP010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.593"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>332
                     /locus_tag="ACHUR4_004530"
     CDS             <1..>332
                     /locus_tag="ACHUR4_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457763.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITR"
BASE COUNT           71 a           88 c           95 g           78 t
ORIGIN      
        1 tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc
       61 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      121 ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca
      181 cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa
      241 ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg
      301 tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAZPFP010000585          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZPFP010000585 DAZPFP010000000
VERSION     DAZPFP010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.594"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            9..>327
                     /locus_tag="ACHUR4_004531"
     CDS             9..>327
                     /locus_tag="ACHUR4_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1457764.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          103 a           78 c           84 g           62 t
ORIGIN      
        1 acaagggaat gaacaatgaa ctccccgatg atattgagct gcttaaagcc atgttgcgta
       61 agcaacagag tcggcttcga cagtatgcct gtcaggtcgc gggctatgag caggaaattg
      121 aacggctgaa agcgcaactc gacaggttgc gtcgtatgtt gttcggccag agttcagaga
      181 aaaagcgtca taagcttgaa aatcagatcc gacaggcaga aaaacgactg tcggaactgg
      241 aaaaccggct gaacacagcc agaaatcttc tggaagatgc atcgtcagtc acagattcac
      301 ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZPFP010000586          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZPFP010000586 DAZPFP010000000
VERSION     DAZPFP010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.595"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>327
                     /locus_tag="ACHUR4_004532"
     CDS             <1..>327
                     /locus_tag="ACHUR4_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFR1457765.1"
                     /translation="PYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDETNMSKFIC
                     KKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATPNDVYAYAN
                     KVRQRI"
BASE COUNT          117 a           55 c           61 g           94 t
ORIGIN      
        1 cccctatggt tattgggggg tcagtgctga taaagtatac attccattat caggtgataa
       61 taaaacaaag gatggtaaaa tcagccataa cctttttggc cttgatgaaa caaacatgtc
      121 aaaatttata tgcaaaaaga aagcagatgc tttcagacaa ttggcaaatt ataaattaat
      181 cagtaaatct gaaaactgtg ctggtatggc acttaatgtt cttaaagcag gaaattctga
      241 aatatacttt ccactccctg acgttaaatt ggttgctaca cctaacgatg tatacgcgta
      301 tgcaaataaa gttaggcaac gcattga
//
LOCUS       DAZPFP010000587          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZPFP010000587 DAZPFP010000000
VERSION     DAZPFP010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.596"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>327
                     /locus_tag="ACHUR4_004533"
     CDS             <1..>327
                     /locus_tag="ACHUR4_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457766.1"
                     /translation="AKIFKYIEIPLPFFYFIYTSGEPFHISVQNTVIYVSKYNGIFIN
                     KLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLFDHDIERLKKISKAQLISPDY
                     VLIDFSS"
BASE COUNT          104 a           51 c           45 g          127 t
ORIGIN      
        1 gcgaaaatat ttaaatacat tgaaatccct ctgccgtttt tctactttat ctatacatca
       61 ggcgaaccat tccacatatc tgtccaaaat actgttattt atgtatcaaa atacaatggt
      121 atatttatta ataaattagt tcctttttct cttttatttg acagggatat atctgttctt
      181 caaagaagag atatttgtgt tgtgcgattt acgtcagagg aaataagtga acataacgtt
      241 ttatttgatc atgacattga acgattaaaa aaaatttcca aagctcagct tatatctccc
      301 gattatgttt tgattgattt ttctagt
//
LOCUS       DAZPFP010000588          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZPFP010000588 DAZPFP010000000
VERSION     DAZPFP010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.597"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHUR4_004534"
     CDS             103..>325
                     /locus_tag="ACHUR4_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1457767.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZPFP010000589          324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZPFP010000589 DAZPFP010000000
VERSION     DAZPFP010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.598"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>324
                     /gene="ipaC"
                     /locus_tag="ACHUR4_004535"
     CDS             <1..>324
                     /gene="ipaC"
                     /locus_tag="ACHUR4_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1457768.1"
                     /translation="NSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRT
                     YELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEAS
                     QVSKEA"
BASE COUNT          119 a           73 c           59 g           73 t
ORIGIN      
        1 aactctagca caaaattttt aggtaaaaat aaactggcgc cagataatat atccctgtca
       61 actgaacata aaacttctct tagttctccc gatatttctt tgcaggataa aattgacacc
      121 cagagaagaa cttacgagct caataccctt tctgcgcagc aaaaacaaaa cattggccgt
      181 gcaacaatgg aaacatcagc cgttgctggt aatatatcca catcaggagg gcgttatgca
      241 tctgctcttg aagaagaaga acaactaatc agtcaggcca gcagtaaaca agcagaggaa
      301 gcatcccaag tatctaaaga agca
//
LOCUS       DAZPFP010000590          321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZPFP010000590 DAZPFP010000000
VERSION     DAZPFP010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.599"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            120..321
                     /locus_tag="ACHUR4_004536"
                     /pseudo
     CDS             120..321
                     /locus_tag="ACHUR4_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT           86 a           78 c           57 g          100 t
ORIGIN      
        1 tttcatctat ttattcacag cgcgaatgaa aaataaaaca ctctgcttta cagtgtttac
       61 tttataatta acagtcttat ctcactgttc accctgtaac gttcagagag cgtcttcaga
      121 tgtctattta taaaattcct cttccgctca atattctgga agctgcacga gagcgtatta
      181 cctagacact caacacccta ccccgcgttt gtgtctcatt ttctggcggc aaagactctg
      241 gtttaatgct gcatctgaca gccgaacttg cccgacaaat gggcaaaaaa atctgcgttt
      301 tgtttatcaa ctgggaggcg c
//
LOCUS       DAZPFP010000591          314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZPFP010000591 DAZPFP010000000
VERSION     DAZPFP010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.601"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           83 a           70 c           74 g           87 t
ORIGIN      
        1 ttaaaaaatg gagcaatatt aggcaaacga tttgagatat gacctaaaag ctcggccaaa
       61 taataatgtc gtcgattatc gaagtcatca tgagtgattc tgtccccaca gtgcagcatc
      121 agcatgacag tgaattgtct ggcgtggtcc atttaatgag atggagatgg tccctctttc
      181 ctaaatagct gtgcctaata ccgctacact tttgccagcc catgtttgcc tccggggaat
      241 gggctgacgg ttccttcaaa atccattgca cgcagttatg tcggagcggg aacttgactc
      301 ctgtctttac ggga
//
LOCUS       DAZPFP010000592          306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZPFP010000592 DAZPFP010000000
VERSION     DAZPFP010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.602"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            229..>306
                     /locus_tag="ACHUR4_004537"
     CDS             229..>306
                     /locus_tag="ACHUR4_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1457769.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT           97 a           54 c           67 g           88 t
ORIGIN      
        1 tattatttgt ttttggatat atatcttgcg ccttgttatg tagttaatgc tcagaatatg
       61 atagataaaa tagagacgca aaacagggca tgctcataac atgatgataa cataaaaaat
      121 tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa agagggtaat gctgccaact
      181 tactgattta gtgtatgatg gtgattttaa ggtgcttgcg tagcttccat ttccatcaga
      241 tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg gcaaaagcac tgccggacat
      301 cagcgc
//
LOCUS       DAZPFP010000593          300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZPFP010000593 DAZPFP010000000
VERSION     DAZPFP010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.603"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>300
                     /locus_tag="ACHUR4_004538"
     CDS             <1..>300
                     /locus_tag="ACHUR4_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1457770.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRN"
BASE COUNT           73 a           75 c           86 g           66 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggatga caaaggacat tgtcctgaac gcactgctga tggctgtatg gcggcgtaat
//
LOCUS       DAZPFP010000594          299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZPFP010000594 DAZPFP010000000
VERSION     DAZPFP010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.604"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHUR4_004539"
     CDS             complement(<1..279)
                     /locus_tag="ACHUR4_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457771.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLF"
BASE COUNT          122 a           67 c           37 g           73 t
ORIGIN      
        1 aaatagtcta aaaaaactca agtcttgtat tttacttctt ttctttataa gaagaaaaaa
       61 taaaatacca atcccccaac cggtaaaaag tatacccatt actatgatag catagagtaa
      121 acttctggca acatcatcca aacctgcacc aacaacccac catggaaatc catagtaaaa
      181 agaggttccc cagccataag aataagcact gacccaccct acacatccca tataggcgat
      241 gaataatgaa gaatttctaa gcaatgaatt gtcattcata agaaactaac tgataatgt
//
LOCUS       DAZPFP010000595          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZPFP010000595 DAZPFP010000000
VERSION     DAZPFP010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.605"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(1..106)
                     /locus_tag="ACHUR4_004540"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="ACHUR4_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            101..292
                     /locus_tag="ACHUR4_004541"
                     /pseudo
     CDS             101..292
                     /locus_tag="ACHUR4_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           73 a           83 c           86 g           50 t
ORIGIN      
        1 cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc catgaaagcc
       61 ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc agcggcggac
      121 agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt acccggtcaa
      181 aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta
      241 acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gc
//
LOCUS       DAZPFP010000596          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZPFP010000596 DAZPFP010000000
VERSION     DAZPFP010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.606"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHUR4_004542"
     CDS             <1..>289
                     /locus_tag="ACHUR4_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1457772.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZPFP010000597          287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZPFP010000597 DAZPFP010000000
VERSION     DAZPFP010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.607"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>287)
                     /locus_tag="ACHUR4_004543"
     CDS             complement(<1..>287)
                     /locus_tag="ACHUR4_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFR1457773.1"
                     /translation="LLYSGLVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAI
                     QALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLL"
BASE COUNT          115 a           56 c           41 g           75 t
ORIGIN      
        1 taataacaga gcatctaaaa ttacacgtat gctccaggat attgcggcac ccagtaaccc
       61 atatttttcg accataaaat acaaaagggc taaataagga accaattcag cacaatgaat
      121 caaagcagta attttagatt ttcctaatgc ctggattgca gagaatggta tcattgccag
      181 cgcattgaag aaaaaaccga tcaataacac atttaatata ttaaaggaat taatttgata
      241 attctcacca aaccatattt tcaatacaag ccctgaatat aacaagc
//
LOCUS       DAZPFP010000598          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZPFP010000598 DAZPFP010000000
VERSION     DAZPFP010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.608"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            18..>286
                     /locus_tag="ACHUR4_004544"
     CDS             18..>286
                     /locus_tag="ACHUR4_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1457774.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKR"
BASE COUNT           88 a           49 c           59 g           90 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaag
//
LOCUS       DAZPFP010000599          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZPFP010000599 DAZPFP010000000
VERSION     DAZPFP010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.609"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            24..100
                     /locus_tag="ACHUR4_004545"
     tRNA            24..100
                     /locus_tag="ACHUR4_004545"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHUR4_004546"
     tRNA            143..218
                     /locus_tag="ACHUR4_004546"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZPFP010000600          274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZPFP010000600 DAZPFP010000000
VERSION     DAZPFP010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.610"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            113..>274
                     /locus_tag="ACHUR4_004547"
     CDS             113..>274
                     /locus_tag="ACHUR4_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457775.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGIT"
BASE COUNT           82 a           54 c           55 g           83 t
ORIGIN      
        1 taataacaaa atagccattg catcgggttg gacaatttac aaaagaaaga taacccatat
       61 tgtataatgc gtttaaatat gccgtcttat ataaaatgat ggtcagatta atatgcggat
      121 tatctttcta cgcaaggagt atttatctct actcccgtca atgattgcat ctcttttctc
      181 tgctaacggt gtcgcggcgg ccattgattt atgccaggga tatgatatca aagcgagttg
      241 tcacgccagc aggcaaagcc tttcaggcat tacg
//
LOCUS       DAZPFP010000601          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZPFP010000601 DAZPFP010000000
VERSION     DAZPFP010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.611"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHUR4_004548"
     CDS             complement(<1..212)
                     /locus_tag="ACHUR4_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457776.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT           82 a           59 c           42 g           89 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tc
//
LOCUS       DAZPFP010000602          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZPFP010000602 DAZPFP010000000
VERSION     DAZPFP010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.612"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..139)
                     /locus_tag="ACHUR4_004549"
     CDS             complement(<1..139)
                     /locus_tag="ACHUR4_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1457777.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT           81 a           45 c           47 g           97 t
ORIGIN      
        1 acgagtttgc gcagtactgc aaacgcggtg actcttctgg cattatttgg caatgcattg
       61 agcaactatt gcatgtaaga cttttataac gactacaaag ccccatgtta agaacctccc
      121 tatccgtata taattgcata atttcattgg tgattgatga tgtcaataca ccttgatttt
      181 gtaattttgc tgggaaaaat tgcgattaat ctgtatttgc tataattctg attaagatat
      241 ttaaaaatgc atttctcgtt attaaatatg
//
LOCUS       DAZPFP010000603          265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZPFP010000603 DAZPFP010000000
VERSION     DAZPFP010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.613"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..62
                     /locus_tag="ACHUR4_004550"
     CDS             <1..62
                     /locus_tag="ACHUR4_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457778.1"
                     /translation="VSKAGIISRRRLLLFQFVG"
     gene            complement(39..191)
                     /gene="hokA"
                     /locus_tag="ACHUR4_004551"
     CDS             complement(39..191)
                     /gene="hokA"
                     /locus_tag="ACHUR4_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1457779.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
BASE COUNT           81 a           61 c           49 g           74 t
ORIGIN      
        1 ccgtaagtaa ggcgggaata atttcccgtc gaagactctt actctttcaa tttgtaggct
       61 aaaaacgccg ccagctcata actctcctgt ttaatatgca attcacacag tgaatctctt
      121 atcatccagg tgaaaaataa aagcgtgaaa caaatcacta ttaaagaaag taatctatat
      181 ttctgcggca ttcccagccc cctgtgttga tttccaacga gtaagaggct aaccttatgg
      241 tgttcaggca tagggtagcc tcact
//
LOCUS       DAZPFP010000604          264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZPFP010000604 DAZPFP010000000
VERSION     DAZPFP010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.614"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           79 a           54 c           68 g           63 t
ORIGIN      
        1 tctaataaaa aaacatagct gcagagcaag caattcggtt gttggcaagt caagggcaaa
       61 gcattcgcgt gagaatgggc gtatgctttg tgccaaaagc aaacgttgct aattctgcac
      121 tgttcagcga tggcgcagta gagacaggca ctgccactgt ccagtcggca aaagcgaaca
      181 ctttgccact gcggggataa actgattgaa gctctcgcgc aaaatcactt attcttttgc
      241 gttaataaaa tgtaggagat gggt
//
LOCUS       DAZPFP010000605          263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZPFP010000605 DAZPFP010000000
VERSION     DAZPFP010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.615"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           72 a           52 c           47 g           92 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gat
//
LOCUS       DAZPFP010000606          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZPFP010000606 DAZPFP010000000
VERSION     DAZPFP010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.616"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHUR4_004552"
     CDS             complement(<1..224)
                     /locus_tag="ACHUR4_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457780.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT           65 a           73 c           60 g           64 t
ORIGIN      
        1 gcagcaagtt cagaggcagg aacgacctgt tctcctgcgg ccacagcagt aagacttccc
       61 tcctggtatt gcttgcgcca gagaaataac tggctggctg ccacaccgtg ttgccgggca
      121 acaagggaga ccgtcattcc cggttcaaaa ctctgctgaa caatagcgat cttttcctgt
      181 gtagtacgcc gtctgcgttt ctccggtcct aagacatcaa tcatctgctc tccaatgact
      241 agtctaaaaa ctagtattaa ga
//
LOCUS       DAZPFP010000607          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZPFP010000607 DAZPFP010000000
VERSION     DAZPFP010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.618"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>258
                     /locus_tag="ACHUR4_004553"
     CDS             <1..>258
                     /locus_tag="ACHUR4_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1457781.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           67 a           71 c           71 g           49 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaat
//
LOCUS       DAZPFP010000608          255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZPFP010000608 DAZPFP010000000
VERSION     DAZPFP010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.619"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           77 a           54 c           52 g           72 t
ORIGIN      
        1 aaaaagtggc cgggtttttt gatttctgtg tttcgtgcgc ttaatcgagt ctcacgcttc
       61 agtgagcaaa ttgcccctcc ccaacagata tcacgattca caaggtcaaa aattctgttc
      121 catgaagtac cgtacaaaga taaagagaaa aatgccttat gcgggacgta gctgtcgcgt
      181 ctttatcggg aaactatacg taattgccac tttgtcttcg gtaatcagaa aaagaagacc
      241 tgccatttta ataaa
//
LOCUS       DAZPFP010000609          252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZPFP010000609 DAZPFP010000000
VERSION     DAZPFP010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.620"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>252
                     /gene="virB"
                     /locus_tag="ACHUR4_004554"
     CDS             <1..>252
                     /gene="virB"
                     /locus_tag="ACHUR4_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1457782.1"
                     /translation="IRELGIGLNFLKVSGMSYKDIAKKENLSRAKVTRAFQAASVPQE
                     IISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPI"
BASE COUNT           85 a           50 c           43 g           74 t
ORIGIN      
        1 gcatccgaga acttggtatt ggtcttaatt ttctgaaagt atcagggatg tcctataaag
       61 acatagccaa aaaagagaat ctgtctcgcg cgaaagtcac tcgtgccttt caggcagcaa
      121 gcgttccaca ggaaataata tctctatttc caatcgcgtc agaacttaac tttaatgact
      181 acaagatatt attcaattat tataaaggac ttgaaaaggc taatgaatct cttagttcta
      241 cactaccaat at
//
LOCUS       DAZPFP010000610          250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZPFP010000610 DAZPFP010000000
VERSION     DAZPFP010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.621"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>250
                     /locus_tag="ACHUR4_004555"
     CDS             <1..>250
                     /locus_tag="ACHUR4_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457783.1"
                     /translation="DTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSAD
                     RKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT           59 a           62 c           83 g           46 t
ORIGIN      
        1 gacactccgg tgaaagtact ggccccgggg aacggaaaga cgaaaacggg tcgtctgtgg
       61 gtatacgtca gggatgatcg taatgcgggt tcatccctgc cggcagccgt ctggttcgcg
      121 tattcggcag atcgcaaagg agaacatccg cagctccacc tggcaaagta tcagggcgta
      181 ctgcaggctg atgcctatgc aggttataac gtactgtacg aaacgggccg ggtgaaggaa
      241 gccgggtgcc
//
LOCUS       DAZPFP010000611          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZPFP010000611 DAZPFP010000000
VERSION     DAZPFP010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.622"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           64 a           61 c           52 g           72 t
ORIGIN      
        1 cataatacgc tttcccgcta cagagtcaag catttatttt tgctttctct gccggaattc
       61 tcaggagaac cccgctgacc cggcggcgtg tttgccgttg ttccgtgtca gtggtggcgc
      121 attataggga gttattccgg cctgacaagc gaaaaatata aaaactttat cgttcgctca
      181 cttttcaggc aaaacatctt aaatatagtc ttttccgtct aacttataga caaaaacgag
      241 ccccgaagg
//
LOCUS       DAZPFP010000612          246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZPFP010000612 DAZPFP010000000
VERSION     DAZPFP010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.623"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(125..199)
                     /gene="tnaC"
                     /locus_tag="ACHUR4_004556"
     CDS             complement(125..199)
                     /gene="tnaC"
                     /locus_tag="ACHUR4_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR1457784.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
BASE COUNT           88 a           43 c           47 g           68 t
ORIGIN      
        1 tttactaaaa taagtaaata aacgagagat ggcctttgca aaaggcaaaa ttaagatata
       61 gcaaactaat cgatcacatt gaatctaatc ggtttggctc tggtgatggc tacagaaggg
      121 taaattaagg gcggtgatcg acaatcttat tgtcgatatt gaaccatttt gaggtcacac
      181 atacatgtaa gatattcata atgcacttat cctcgaaaga cacgccgcta cattattaca
      241 ctcaga
//
LOCUS       DAZPFP010000613          245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZPFP010000613 DAZPFP010000000
VERSION     DAZPFP010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.624"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>245)
                     /gene="mxiM"
                     /locus_tag="ACHUR4_004557"
     CDS             complement(<1..>245)
                     /gene="mxiM"
                     /locus_tag="ACHUR4_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR1457785.1"
                     /translation="TTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQK
                     IAIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKL"
BASE COUNT           76 a           53 c           23 g           93 t
ORIGIN      
        1 attaatttta atttaacttc cccatccttc tctataagga tggtatcatt tatcttatta
       61 ccttttaaaa caaccccgtc tttcagcata gaaaatagtt tatctatagc tatcttttgt
      121 gcatccatga agcacccatc tacttcctta acattaaagt tgcccatgaa tttacctgca
      181 ttaaaactat aaaccgcctt accactaata catttagagt aaacatttat acttttattg
      241 gttgt
//
LOCUS       DAZPFP010000614          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZPFP010000614 DAZPFP010000000
VERSION     DAZPFP010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.625"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUR4_004558"
     CDS             <1..216
                     /locus_tag="ACHUR4_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457786.1"
                     /translation="NFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVGP
                     GTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT           77 a           49 c           44 g           73 t
ORIGIN      
        1 aattttatcg cattacaaat aaatggttca aaaacaggat ttgccgcatc tattacaggt
       61 tttactcaaa caataagcgc tggtacaatc agtttcctgt cagtaaccgt aagcgacaac
      121 ggtgttggtc ccggaactac tttaggtctt ggttcactaa taacttcatt actgtcaata
      181 gccgtaatcg ccattgcagg aaataataaa aagtaagtgt actctgactg cgtatgtatc
      241 tac
//
LOCUS       DAZPFP010000615          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZPFP010000615 DAZPFP010000000
VERSION     DAZPFP010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.626"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>243
                     /locus_tag="ACHUR4_004559"
     CDS             <1..>243
                     /locus_tag="ACHUR4_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1457787.1"
                     /translation="RGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNG
                     RSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT           50 a           65 c           66 g           62 t
ORIGIN      
        1 cgtggcggaa tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag
       61 gagcctgccg ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact
      121 atgcgtaatg gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg
      181 ttgtacatcg aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat
      241 gcg
//
LOCUS       DAZPFP010000616          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZPFP010000616 DAZPFP010000000
VERSION     DAZPFP010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.627"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..138)
                     /locus_tag="ACHUR4_004560"
     CDS             complement(<1..138)
                     /locus_tag="ACHUR4_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFR1457788.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QV"
BASE COUNT           97 a           56 c           33 g           55 t
ORIGIN      
        1 aacctgctgc aaaccaagtg catttcgtac cattacaaaa acaatagaaa actttatata
       61 acaggtacca gcagcaacta aaaatggtaa tagcgtaaaa aaagaaagtg tagcgatgag
      121 ggacatgtca ctcagcatga gattactcct ttaccatcca agaactaatc tcgataccat
      181 aaccatcctc aatagaaaca agttccccat gcccaaccaa agaaccattt actttaatat
      241 t
//
LOCUS       DAZPFP010000617          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZPFP010000617 DAZPFP010000000
VERSION     DAZPFP010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.628"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>241)
                     /locus_tag="ACHUR4_004561"
     CDS             complement(<1..>241)
                     /locus_tag="ACHUR4_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1457789.1"
                     /translation="ALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNE
                     LIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGS"
BASE COUNT           88 a           56 c           32 g           65 t
ORIGIN      
        1 gctaccgtta aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac
       61 atcaattacc actgccccat attcatcttt aaactctaat gctattaact cgttcaatga
      121 acccttacag acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata
      181 atcaaatgaa agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc
      241 c
//
LOCUS       DAZPFP010000618          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZPFP010000618 DAZPFP010000000
VERSION     DAZPFP010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.629"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..230
                     /locus_tag="ACHUR4_004562"
     CDS             <1..230
                     /locus_tag="ACHUR4_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1457790.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT           66 a           53 c           69 g           53 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 a
//
LOCUS       DAZPFP010000619          238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZPFP010000619 DAZPFP010000000
VERSION     DAZPFP010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.630"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           79 a           44 c           55 g           60 t
ORIGIN      
        1 tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt agcacgtcgt atgcaagaac
       61 gtgccacggc tggctgatgg atgttcgata gcgcgagttt gaatgaaaat cagccggaga
      121 tgattttaca taattgctac ggaattattc aatacaggaa ttgcttgcgt ctgcatggat
      181 tgacctgaaa tattcccgaa aatttctcta aaaaactcga aaaaaatggt aactggtt
//
LOCUS       DAZPFP010000620          237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZPFP010000620 DAZPFP010000000
VERSION     DAZPFP010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.631"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>237)
                     /locus_tag="ACHUR4_004563"
     CDS             complement(<1..>237)
                     /locus_tag="ACHUR4_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457791.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT           44 a           80 c           50 g           63 t
ORIGIN      
        1 tcacgactca ggaactgttt caccatttgt gcatagcaca ctttcgccac tggttccggt
       61 ttatccatcc ccagagcacg gtacacctgc ggcagcttct ctccacacac ccggttggca
      121 aggaacccga agtacctcac catcttaaaa aacttctccg ggatgtgctg tttcagcctc
      181 gcgaccagct cacgctgtgt cagcgtttcc gtcgccgttt ctcccgtttt gtggtcc
//
LOCUS       DAZPFP010000621          235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZPFP010000621 DAZPFP010000000
VERSION     DAZPFP010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.632"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>235)
                     /locus_tag="ACHUR4_004564"
     CDS             complement(<1..>235)
                     /locus_tag="ACHUR4_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457792.1"
                     /translation="LIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQCDCDEI
                     AESNFNRMIAYMSNINYDASKLGAPALSFDTYL"
BASE COUNT           73 a           46 c           45 g           71 t
ORIGIN      
        1 tgcagatacg tatcaaaact aagtgctggc gctcctagct ttgaggcatc atagttaatg
       61 ttagacatat aggcaatcat acgattgaag ttcgactctg ctatttcatc gcagtcacat
      121 tgacccgttg atataaagaa agtgagtgcc gccatggtag actcaagcct ttctttaaaa
      181 gagtcgaaag ccaggctttt aatatttaaa tcaccataat tactctgtat taagt
//
LOCUS       DAZPFP010000622          234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZPFP010000622 DAZPFP010000000
VERSION     DAZPFP010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.633"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(22..>234)
                     /locus_tag="ACHUR4_004565"
     CDS             complement(22..>234)
                     /locus_tag="ACHUR4_004565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457793.1"
                     /translation="PDGLNEPVTFQVSLAGNPCVIRLVAQVCMWLKFIIRDRGGFSGG
                     LLLFLPVCCRDRTERILAVHTIKILR"
BASE COUNT           75 a           64 c           49 g           46 t
ORIGIN      
        1 ggggaccatt ccctcgcgat attaacgtaa tatcttaatt gtatgcaccg ccagaatgcg
       61 ttcagtgcga tcacggcagc agacaggtaa aaatagcaac aaaccacccg aaaaaccgcc
      121 gcgatcgcgt atgataaatt ttaaccacat gcatacctgt gcaacaaggc gaatcacgca
      181 cggatttcca gccagagaca cctgaaacgt aaccggctca tttaaaccgt ctgg
//
LOCUS       DAZPFP010000623          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZPFP010000623 DAZPFP010000000
VERSION     DAZPFP010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.634"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>231
                     /gene="virF"
                     /locus_tag="ACHUR4_004566"
     CDS             <1..>231
                     /gene="virF"
                     /locus_tag="ACHUR4_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR1457794.1"
                     /translation="EPIYSFQHSYSEEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGK
                     RKIYSLACLLSAVSDEEALYTSISIASSLSFSD"
BASE COUNT           68 a           40 c           41 g           82 t
ORIGIN      
        1 gaaccaattt attcatttca acactcctat tctgaggaga aaagggggtt aaacaaaaaa
       61 atattcctcc tctctgagga ggaggtttct atcgatttgt tcaaatctat aaaagagatg
      121 cctttcggca aaagaaagat ctatagttta gcttgccttt tatcagctgt ttctgatgag
      181 gaagctttat atacttcgat atcgatagct tcttctctta gtttttctga t
//
LOCUS       DAZPFP010000624          230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZPFP010000624 DAZPFP010000000
VERSION     DAZPFP010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.635"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>230)
                     /locus_tag="ACHUR4_004567"
     CDS             complement(<1..>230)
                     /locus_tag="ACHUR4_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1457795.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT           56 a           59 c           42 g           73 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc
//
LOCUS       DAZPFP010000625          229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZPFP010000625 DAZPFP010000000
VERSION     DAZPFP010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.636"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            20..>229
                     /locus_tag="ACHUR4_004568"
     CDS             20..>229
                     /locus_tag="ACHUR4_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1457796.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKS"
BASE COUNT           54 a           60 c           63 g           52 t
ORIGIN      
        1 gagtcgcccc gcccttctga tgctcccccg ttttgccgat atttttcagc agggaaaccg
       61 ctggcttaac tggctggaga aacaaccgga aggggcagtc cgtccggtgg taattgaatc
      121 agtgacaaaa atcatggcct gcgggaccac actgatgggc tacacgcagt ggtgttgttc
      181 atcaccggac tgttgccata caaaaaaggt ctgcttccgg tgtaaaagc
//
LOCUS       DAZPFP010000626          229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZPFP010000626 DAZPFP010000000
VERSION     DAZPFP010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.637"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>229)
                     /locus_tag="ACHUR4_004569"
     CDS             complement(<1..>229)
                     /locus_tag="ACHUR4_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1457797.1"
                     /translation="DALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           43 a           68 c           55 g           63 t
ORIGIN      
        1 gcacggtttt tccagctctt acggtgtatt acctccgctt tgtaaagacc attgatgctc
       61 tccgccatcg cgttgtcata cgagtcgcct gtacttcctg ttgatgccag taatccggct
      121 tccttaagcc gctgtgtgta ggccagcgat acatactgag aacctttatc actgtgatgg
      181 accgtgccgg acggtcgacg ggcccataac gcctgctcca gtgcatcca
//
LOCUS       DAZPFP010000627          226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZPFP010000627 DAZPFP010000000
VERSION     DAZPFP010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.638"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            15..>226
                     /locus_tag="ACHUR4_004570"
     CDS             15..>226
                     /locus_tag="ACHUR4_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1457798.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECAD"
BASE COUNT           58 a           40 c           64 g           64 t
ORIGIN      
        1 atttagagga atctatgcga gggaaaaaac gcattgggtt attgtttttg ctgatagcgg
       61 ttgtggttgg tggcggcggg ttattgctgg cgcaaaaagc cttacataaa acgtcggata
      121 cagcattttg cctttcctgc cactcgatga gtaaaccttt tgaggaatat cagggaactg
      181 tccacttttc gaaccagaaa gggatacgtg cggaatgtgc cgattg
//
LOCUS       DAZPFP010000628          224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZPFP010000628 DAZPFP010000000
VERSION     DAZPFP010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.640"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            26..181
                     /locus_tag="ACHUR4_004571"
                     /pseudo
     CDS             26..181
                     /locus_tag="ACHUR4_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           74 a           50 c           52 g           48 t
ORIGIN      
        1 ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc ttgcggctga
       61 actggctaaa ggcctcaaaa ccgaggccga cctcaatcag ttttcccgta tgcagacgaa
      121 attaactgtc gaaacggtgc ttaatgcgga actaactgac catctcgggc atgaaaaaag
      181 ctacatccgg tagtaaacat tatcagtggg ttatgcgata tgat
//
LOCUS       DAZPFP010000629          221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZPFP010000629 DAZPFP010000000
VERSION     DAZPFP010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.641"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           66 a           57 c           43 g           55 t
ORIGIN      
        1 gaaagtcttc atcaacaaca agtaaaaaca acgtccctct catcgaaccg gacttgcaga
       61 tttcccgcat ccggctctct tgcaacacat cagaccttca cgttcgggcg gttatgggga
      121 aggtccagca agctgtatga gccctaactg tttgaatacc agtagattac cagaaacttc
      181 tatcgattga agacattacc aactagcgtt actaaatgtt g
//
LOCUS       DAZPFP010000630          219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZPFP010000630 DAZPFP010000000
VERSION     DAZPFP010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.642"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>219
                     /locus_tag="ACHUR4_004572"
     CDS             <1..>219
                     /locus_tag="ACHUR4_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFR1457799.1"
                     /translation="TTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALEN
                     EMIIFTSPDLKPRIEAIRNGKPTTVIVI"
BASE COUNT           63 a           42 c           47 g           67 t
ORIGIN      
        1 ccaccacaat tatcactgca tattttgaca ttggtagagg ggattggact gctaataaag
       61 ggttccgtga aaaactagct cgttcagttg atgtttattt tagttacttt gagcgtctgg
      121 ccgcacttga aaatgagatg attattttca cttctcccga tttgaaacca aggattgaag
      181 cgattcgcaa cggaaaacca acaacggtta tcgttatcg
//
LOCUS       DAZPFP010000631          218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZPFP010000631 DAZPFP010000000
VERSION     DAZPFP010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.643"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>218
                     /locus_tag="ACHUR4_004573"
     CDS             <1..>218
                     /locus_tag="ACHUR4_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457800.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPG"
BASE COUNT           57 a           60 c           49 g           52 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggac
//
LOCUS       DAZPFP010000632          216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZPFP010000632 DAZPFP010000000
VERSION     DAZPFP010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.644"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           54 a           44 c           54 g           64 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtac
//
LOCUS       DAZPFP010000633          214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZPFP010000633 DAZPFP010000000
VERSION     DAZPFP010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.645"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>214
                     /locus_tag="ACHUR4_004574"
     CDS             <1..>214
                     /locus_tag="ACHUR4_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR1457801.1"
                     /translation="DEVKEIAEIIDDKRYGIVNTGQCNYILAETQNDAVWASVALNKT
                     GFTKCRYILVSNKEINRIQQYINQRFP"
BASE COUNT           89 a           32 c           39 g           54 t
ORIGIN      
        1 aagatgaagt aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca
       61 ctggacagtg caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag
      121 ccttaaataa aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa
      181 atagaattca acaatatatc aatcaacgat tccc
//
LOCUS       DAZPFP010000634          214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZPFP010000634 DAZPFP010000000
VERSION     DAZPFP010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.646"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>214
                     /locus_tag="ACHUR4_004575"
     CDS             <1..>214
                     /locus_tag="ACHUR4_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1457802.1"
                     /translation="MRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYM
                     SKKTAGGRNTARYLGRYLKKPPIAASR"
BASE COUNT           54 a           51 c           74 g           35 t
ORIGIN      
        1 atgcggcaac ggcttctgaa agcgtggtca gaggggctgg cgatgccgga gtcgttgtca
       61 catatcacga cggaatcaca gaggagaagc ctggtgctga aagccggagg aaaatactgg
      121 catgtgtaca tgtcgaaaaa aacggccggc gggaggaata cggcgcgcta cctgggtcgt
      181 tatctgaaga agccgccgat agcggcctcc cggc
//
LOCUS       DAZPFP010000635          211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZPFP010000635 DAZPFP010000000
VERSION     DAZPFP010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.647"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>211
                     /locus_tag="ACHUR4_004576"
     CDS             <1..>211
                     /locus_tag="ACHUR4_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1457803.1"
                     /translation="LFNLFSPALKLNTGLAILSPGAFEVHSDGIDADNELFHYTIKKA
                     YTPYNIHTYKTEEVVNQRNIKVKNMT"
BASE COUNT           74 a           40 c           34 g           63 t
ORIGIN      
        1 ttatttaatt tgttttcccc agcacttaag cttaatactg gcctggcaat tctttcgcct
       61 ggtgcatttg aagttcactc tgacggaata gatgcggata acgaattgtt tcactatact
      121 attaaaaaag catatacccc atataatata cacacttaca agacagagga agttgtaaac
      181 cagaggaata taaaagttaa aaacatgacc t
//
LOCUS       DAZPFP010000636          210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZPFP010000636 DAZPFP010000000
VERSION     DAZPFP010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.648"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..>210)
                     /locus_tag="ACHUR4_004577"
     CDS             complement(<1..>210)
                     /locus_tag="ACHUR4_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR1457804.1"
                     /translation="NTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANA
                     YIIDHDSPYGYMTLTDHFDNAIPPVF"
BASE COUNT           71 a           31 c           42 g           66 t
ORIGIN      
        1 aaaaacaggt ggaatagcat tatcaaagtg gtcggttaat gtcatatatc catatggaga
       61 gtcatgatca atgatatacg cattagcacc aaaatccaca gtatgatgtg ttttatttag
      121 tgggagtgtt tcttgatgat tggaaatttc aaccccaaaa aaaacaaagt cgtggttagc
      181 tatttcttca atatcctcag agaatgtgtt
//
LOCUS       DAZPFP010000637          210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZPFP010000637 DAZPFP010000000
VERSION     DAZPFP010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.650"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>210
                     /locus_tag="ACHUR4_004578"
     CDS             <1..>210
                     /locus_tag="ACHUR4_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1457805.1"
                     /translation="AMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNE
                     NIVLLGPSGVGKTHLAIAMGYEAVR"
BASE COUNT           58 a           53 c           56 g           43 t
ORIGIN      
        1 aggcgatgta tacccgaatg gcagccttcc cggcggtgaa aacgttcgaa gagtatgact
       61 tcacattcgc caccggagca ccgcagaagc aactccagtc gttacgctca ctcagcttca
      121 tagaacgtaa tgaaaatatc gtattactgg ggccatcagg tgtggggaaa acccatctgg
      181 caatagcgat gggctatgaa gcagtccgtg
//
LOCUS       DAZPFP010000638          209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZPFP010000638 DAZPFP010000000
VERSION     DAZPFP010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.651"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(<1..193)
                     /locus_tag="ACHUR4_004579"
     CDS             complement(<1..193)
                     /locus_tag="ACHUR4_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1457806.1"
                     /translation="MIFNMINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKG
                     ELSTWDTIYLHLILAINADS"
BASE COUNT           71 a           38 c           36 g           64 t
ORIGIN      
        1 cactgtcagc atttattgct aatatcagat gcaagtagat ggtatcccat gtagacaact
       61 cacccttacg actgggattt aatatcgcga aaaattccct cagtgaatcc aggaaattat
      121 cactttgatt tagttgtgtg cctggaaatg atgatgcttg atcagatata ttgtttatca
      181 tattaaaaat cacccaagaa aaacgataa
//
LOCUS       DAZPFP010000639          209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZPFP010000639 DAZPFP010000000
VERSION     DAZPFP010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.652"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           71 a           57 c           21 g           60 t
ORIGIN      
        1 ctctgcaaaa tatgtcagcc ttctcaaact taccagcact taaatattta agcacacaat
       61 cattccttga gggagttcac ttatttcaag agaaacattt cgtcttccca cacaactaat
      121 ccgcccaata tagctaaatt tgtactcagt aatatcattt gttatgcccc cccaccccaa
      181 aatattcaag ctccttcaca gataaaaaa
//
LOCUS       DAZPFP010000640          209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZPFP010000640 DAZPFP010000000
VERSION     DAZPFP010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.653"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
BASE COUNT           38 a           67 c           53 g           51 t
ORIGIN      
        1 gccccttcct cgggaggggc tttcccgttt cagccccttg ctgactcccc gattgttgat
       61 gagcgccgga ccccgcgcaa cgctttccct gaagcagttt tgccttcccc gagcggtcac
      121 gcaaaacgct gcaacgtcct gagcatttgc accttttagc ggaacgcaga tataaaaaat
      181 ccccggcttt ttgggccagg gatatgtgt
//
LOCUS       DAZPFP010000641          209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZPFP010000641 DAZPFP010000000
VERSION     DAZPFP010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.denovo.654"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..>209
                     /locus_tag="ACHUR4_004580"
     CDS             <1..>209
                     /locus_tag="ACHUR4_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1457807.1"
                     /translation="ANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSF
                     RSLEHLDKVSRHYISEIIQKVHPLS"
BASE COUNT           63 a           44 c           38 g           64 t
ORIGIN      
        1 gtgctaatac tgtgaaaaac tttttaagga agtctatagc agcgcaaagt tatagcaaaa
       61 tgttttctca agggaccagt ttcaaatcac taaatctttc tcttgaagcg ccatctggag
      121 cacggtcatc atttcggtca cttgagcatc tcgataaggt gtccaggcac tatatctctg
      181 agataataca aaaagttcat cctctttct
//
LOCUS       DAZPFP010000642         9712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.141, whole genome shotgun sequence.
ACCESSION   DAZPFP010000642 DAZPFP010000000
VERSION     DAZPFP010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.141"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            complement(35..205)
                     /locus_tag="ACHUR4_004581"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHUR4_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHUR4_004582"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHUR4_004582"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1457808.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHUR4_004583"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHUR4_004583"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1457809.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHUR4_004584"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHUR4_004584"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1457810.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHUR4_004585"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHUR4_004585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1457811.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHUR4_004586"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHUR4_004586"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1457812.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6232
                     /locus_tag="ACHUR4_004587"
                     /pseudo
     CDS             5100..6232
                     /locus_tag="ACHUR4_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUR4_004588"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUR4_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1457813.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUR4_004589"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUR4_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1457814.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHUR4_004590"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHUR4_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1457815.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHUR4_004591"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHUR4_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1457816.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHUR4_004592"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHUR4_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1457817.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="ACHUR4_004593"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="ACHUR4_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1457818.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2484 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct ttgcacgttg
     9601 cataaccttt aattattcat atgcattgct gttgttactt tttatatagc taacaccaat
     9661 aattaacctt tcttttcggt aatgactcca acttactgat agtgttttat gt
//
LOCUS       DAZPFP010000643         7414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.178, whole genome shotgun sequence.
ACCESSION   DAZPFP010000643 DAZPFP010000000
VERSION     DAZPFP010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.178"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHUR4_004594"
     CDS             263..922
                     /locus_tag="ACHUR4_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1457819.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHUR4_004595"
     CDS             complement(1123..1500)
                     /locus_tag="ACHUR4_004595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1457820.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHUR4_004596"
     CDS             complement(1567..4533)
                     /locus_tag="ACHUR4_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1457821.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHUR4_004597"
     CDS             complement(4536..5096)
                     /locus_tag="ACHUR4_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1457822.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHUR4_004598"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHUR4_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUR4_004599"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUR4_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1457823.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..>7414
                     /locus_tag="ACHUR4_004600"
     CDS             6998..>7414
                     /locus_tag="ACHUR4_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1457824.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYL"
BASE COUNT         1589 a         2040 c         2150 g         1635 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctc
//
LOCUS       DAZPFP010000644         7053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.189, whole genome shotgun sequence.
ACCESSION   DAZPFP010000644 DAZPFP010000000
VERSION     DAZPFP010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.189"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHUR4_004601"
     CDS             59..1102
                     /locus_tag="ACHUR4_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1457825.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUR4_004602"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUR4_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUR4_004603"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUR4_004603"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1457826.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUR4_004604"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUR4_004604"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1457827.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUR4_004605"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUR4_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1457828.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHUR4_004606"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHUR4_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1457829.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUR4_004607"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUR4_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1457830.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAZPFP010000645         4295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.236, whole genome shotgun sequence.
ACCESSION   DAZPFP010000645 DAZPFP010000000
VERSION     DAZPFP010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.236"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUR4_004608"
     CDS             558..797
                     /locus_tag="ACHUR4_004608"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1457831.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUR4_004609"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUR4_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004610"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1457832.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUR4_004611"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUR4_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1457833.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004612"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAZPFP010000646         4292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.237, whole genome shotgun sequence.
ACCESSION   DAZPFP010000646 DAZPFP010000000
VERSION     DAZPFP010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.237"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUR4_004613"
     CDS             558..797
                     /locus_tag="ACHUR4_004613"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1457834.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUR4_004614"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUR4_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004615"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUR4_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1457835.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUR4_004616"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUR4_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1457836.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004617"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZPFP010000647         2501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.311, whole genome shotgun sequence.
ACCESSION   DAZPFP010000647 DAZPFP010000000
VERSION     DAZPFP010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.311"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUR4_004618"
     CDS             <1..190
                     /locus_tag="ACHUR4_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1457837.1"
                     /translation="HSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYR
                     EVTITEKGYAAFKTHFHI"
     gene            complement(168..791)
                     /gene="tetR(B)"
                     /locus_tag="ACHUR4_004619"
     CDS             complement(168..791)
                     /gene="tetR(B)"
                     /locus_tag="ACHUR4_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1457838.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            873..2078
                     /gene="tet(B)"
                     /locus_tag="ACHUR4_004620"
     CDS             873..2078
                     /gene="tet(B)"
                     /locus_tag="ACHUR4_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1457839.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2191..>2501)
                     /locus_tag="ACHUR4_004621"
     CDS             complement(2191..>2501)
                     /locus_tag="ACHUR4_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1457840.1"
                     /translation="YLDYIIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVS
                     KLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK
                     "
BASE COUNT          642 a          478 c          530 g          851 t
ORIGIN      
        1 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
       61 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      121 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      181 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      241 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      301 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      361 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      421 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      481 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      541 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      601 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      661 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      721 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
      781 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
      841 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
      901 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
      961 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1021 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1081 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1141 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1201 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1261 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1321 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1381 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1441 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1501 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1561 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1621 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1681 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1741 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     1801 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     1861 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     1921 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     1981 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2041 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2101 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2161 agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca ataaaaagcc
     2221 gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt ttttcgcatg
     2281 tattgcgata tgcataaacc agccattgag taagttttta agcacatcat catcataagc
     2341 tttaagttgg ttctcttgga tcaatttgct gacaatggcg tttaccttac cagtaatgta
     2401 ttcaaggcta attttttcaa gttcattcca accaatgata ggcatcactt cttggatagg
     2461 gataaggttt ttattattat caataatata atcaagataa t
//
LOCUS       DAZPFP010000648         1360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.388, whole genome shotgun sequence.
ACCESSION   DAZPFP010000648 DAZPFP010000000
VERSION     DAZPFP010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.388"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHUR4_004622"
     CDS             <1..396
                     /locus_tag="ACHUR4_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1457841.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            509..1347
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004623"
                     /pseudo
     CDS             509..1347
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          404 a          317 c          328 g          311 t
ORIGIN      
        1 aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc atggctcgaa
       61 agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat tattaatcac
      121 aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta tctacaagat
      181 ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc aaatagaacc
      241 ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa atatgttttt
      301 gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa agccaagaaa
      361 aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca
      421 acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg
      481 gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac
      541 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      601 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      661 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      721 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      781 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      841 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      901 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      961 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1021 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1081 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1141 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     1201 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca
     1261 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag
     1321 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAZPFP010000649         1357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1805151
            SAMN43277237-rid23488073.guided.389, whole genome shotgun sequence.
ACCESSION   DAZPFP010000649 DAZPFP010000000
VERSION     DAZPFP010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277237
            Sequence Read Archive: SRR30421696
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396356.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,625
            CDSs (total)                      :: 4,545
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 222 of 474
            Pseudo Genes (incomplete)         :: 152 of 474
            Pseudo Genes (internal stop)      :: 164 of 474
            Pseudo Genes (multiple problems)  :: 60 of 474
            Pseudo Genes (short protein)      :: 1 of 474
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277237-rid23488073.guided.389"
                     /strain="M1805151"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-27"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHUR4_004624"
     CDS             <1..396
                     /locus_tag="ACHUR4_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1457842.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            509..1344
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004625"
                     /pseudo
     CDS             509..1344
                     /gene="aadA1"
                     /locus_tag="ACHUR4_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          401 a          317 c          328 g          311 t
ORIGIN      
        1 aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc atggctcgaa
       61 agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat tattaatcac
      121 aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta tctacaagat
      181 ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc aaatagaacc
      241 ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa atatgttttt
      301 gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa agccaagaaa
      361 aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca
      421 acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg
      481 gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac
      541 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      601 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      661 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      721 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      781 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      841 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      901 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      961 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1021 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1081 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1141 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta
     1201 tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta
     1261 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     1321 acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
